FvH4_6g28170

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001297
Physical Location & Seq
Reverse (-)
21697658 .. 21701268
3611 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g28170.t1

Sequence Viewer

Length: 1101 bp
ATGGCTTCATCAAAACCAGCCAACTGCTTTGGCCCCTTTGATCATCACTCATCAGGAGTCAGTATTATTGAGGAACTCATCACCGATGATGATTCGGTGATCCAAGTCCCTCAAAACTATGTTATCCCAGATCAGGAGTCTCCAATCTCTGATGGGACTAACCTTGCCACAATCCCCACCATTGACATGACACAATTGGTCCTACGCAATCAGTCTGCAAACAACCCTGAACTTGAGAAATTGCACTCGGCTTGCAAAGAATGGGGAATTTTTCAGTTGGTTAACCATGGAGTTAGCTCTGCACTGCTGGACAAAGTTAAGCAAGAATTAGAGGATTTTTTCAAGCTTCCCTTGGAAGAGAAAATGAGATACAAAGTAAGGCCAGGTGATGTGCTTGTTGAAGGCTATGGAACCGTTGTCAAATCCGAAGATCAAAAACTCGACTGGGGTAACAGGTTATTCATGTTTACCAACCCTATCAATAGAAGGAAATCCCATCTTTTCCCAGAGCTCCCTATATCCCTCAGGAATGCCTTGGAGAGCTACATGTTGGAATTACAAAGGACTGCCCGGACCCTTTTAGGGTTGATTGGGAAACTTCTAAAGATGAAAATGGAAGAGATGGAAGAGTTATTTGAAGATGGGTTGCAGTCAGTGAGGATGACTCACTATCCCCCATGCCCAAAACCAGAGCTAGTTGTTGGCCTGAAGCCTCACTCTGATGGTAGCGGCATCACTATTCTCCACCAGGTTAATGGAGTTCAAGGCCTTCAAATTAAAAAAGATGGGGTTTGGTTCCCTGTGAGCTTTGATCATGAGGCCTTTGTTGTAAATATTGGAGACATTCTAGAGATTCTGAGTGATGGGGTATATAAAAGCATTGAGCACAGAGTAACAGTGAACTCGGAGAAAGAAAGGATTTCAATTGCTATGTTCTTCTTGCCCAAAGTTGACGCAGAAATTGGACCTGTGACGGCTCTGAGCGATGCTGATCAGAAGCCACCATCTTCTGGGAAAATAGGAATGGAAAAATTTCTCGAGAAGTTCTTCGCTCATAACCTTAATGGAAAAACACCCTTGGAGCGAATGACAACAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

367

Amino Acids

41.1

Weight (kDa)

5.45

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 58 - 160 8.6e-27 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 218 - 315 6e-31 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1010
AciI CCGC 1 cut(s) 729
AclWI GGATC 1 cut(s) 94
AcsI RAATTY 2 cut(s) 267, 1031
AcuI CTGAAG 1 cut(s) 728
AfiI CCNNNNNNNGG 3 cut(s) 133, 582, 1010
AflIII ACRYGT 1 cut(s) 546
AgsI TTSAA 6 cut(s) 343, 401, 638, 764, 773, 924
AjnI CCWGG 2 cut(s) 382, 747
AluBI AGCT 6 cut(s) 297, 346, 511, 543, 694, 807
AluI AGCT 6 cut(s) 297, 346, 511, 543, 694, 807
Alw21I GWGCWC 2 cut(s) 513, 888
Alw26I GTCTC 2 cut(s) 144, 834
AlwI GGATC 1 cut(s) 94
Ama87I CYCGRG 1 cut(s) 1037
AoxI GGCC 5 cut(s) 31, 380, 703, 766, 819
ApoI RAATTY 2 cut(s) 267, 1031
Asp700I GAANNNNTTC 2 cut(s) 1032, 1046
AspS9I GGNCC 4 cut(s) 32, 199, 573, 965
AsuC2I CCSGG 1 cut(s) 571
AsuHPI GGTGA 3 cut(s) 73, 109, 398
AvaI CYCGRG 1 cut(s) 1037
AvaII GGWCC 3 cut(s) 199, 573, 965
AxyI CCTNAGG 1 cut(s) 524
BanII GRGCYC 1 cut(s) 513
Bbv12I GWGCWC 2 cut(s) 513, 888
BccI CCATC 8 cut(s) 146, 504, 616, 635, 716, 779, 857, 1012
BceAI ACGGC 1 cut(s) 990
BciT130I CCWGG 2 cut(s) 384, 749
BclI TGATCA 3 cut(s) 40, 811, 991
BcnI CCSGG 1 cut(s) 571
BcoDI GTCTC 2 cut(s) 144, 834
BfaI CTAG 2 cut(s) 695, 848
BisI GCNGC 1 cut(s) 730
BlsI GCNGC 1 cut(s) 731
Bme1390I CCNGG 3 cut(s) 384, 571, 749
Bme18I GGWCC 3 cut(s) 199, 573, 965
BmeT110I CYCGRG 1 cut(s) 1037
BmgT120I GGNCC 4 cut(s) 32, 199, 573, 965
BmiI GGNNCC 4 cut(s) 34, 412, 575, 797
BmrFI CCNGG 3 cut(s) 384, 571, 749
BmrI ACTGGG 1 cut(s) 454
BmsI GCATC 2 cut(s) 741, 976
BmuI ACTGGG 1 cut(s) 454
BplI GAGNNNNNCTC 2 cut(s) 649, 681
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 571
BsaBI GATNNNNATC 1 cut(s) 990
BsaJI CCNNGG 4 cut(s) 286, 351, 534, 1077
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 582, 1010
Bse1I ACTGG 1 cut(s) 449
Bse21I CCTNAGG 1 cut(s) 524
Bse8I GATNNNNATC 1 cut(s) 990
BseBI CCWGG 2 cut(s) 384, 749
BseDI CCNNGG 4 cut(s) 286, 351, 534, 1077
BseGI GGATG 1 cut(s) 666
BseJI GATNNNNATC 1 cut(s) 990
BseLI CCNNNNNNNGG 3 cut(s) 133, 582, 1010
BseMII CTCAG 3 cut(s) 538, 848, 971
BseNI ACTGG 1 cut(s) 449
BsgI GTGCAG 1 cut(s) 285
BshFI GGCC 5 cut(s) 33, 382, 705, 768, 821
BsiHKAI GWGCWC 2 cut(s) 513, 888
BsiHKCI CYCGRG 1 cut(s) 1037
BsiSI CCGG 1 cut(s) 571
BslFI GGGAC 2 cut(s) 92, 169
BslI CCNNNNNNNGG 3 cut(s) 133, 582, 1010
BsmAI GTCTC 2 cut(s) 144, 834
BsmFI GGGAC 2 cut(s) 92, 169
BsmI GAATGC 1 cut(s) 535
BsnI GGCC 5 cut(s) 33, 382, 705, 768, 821
BsoBI CYCGRG 1 cut(s) 1037
Bsp1286I GDGCHC 2 cut(s) 513, 888
Bsp143I GATC 6 cut(s) 40, 99, 130, 430, 811, 991
Bsp19I CCATGG 1 cut(s) 286
BspACI CCGC 1 cut(s) 729
BspANI GGCC 5 cut(s) 33, 382, 705, 768, 821
BspCNI CTCAG 3 cut(s) 537, 849, 972
BspHI TCATGA 1 cut(s) 814
BspLI GGNNCC 4 cut(s) 34, 412, 575, 797
BspPI GGATC 1 cut(s) 94
BsrI ACTGG 1 cut(s) 449
BssECI CCNNGG 4 cut(s) 286, 351, 534, 1077
BssMI GATC 6 cut(s) 40, 99, 130, 430, 811, 991
BssT1I CCWWGG 4 cut(s) 286, 351, 534, 1077
Bst2UI CCWGG 2 cut(s) 384, 749
Bst4CI ACNGT 2 cut(s) 415, 898
Bst6I CTCTTC 3 cut(s) 351, 612, 621
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 3 cut(s) 524, 857, 980
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 1 cut(s) 666
BstKTI GATC 6 cut(s) 43, 102, 133, 433, 814, 994
BstMAI GTCTC 2 cut(s) 144, 834
BstMBI GATC 6 cut(s) 40, 99, 130, 430, 811, 991
BstNI CCWGG 2 cut(s) 384, 749
BstNSI RCATGY 1 cut(s) 550
BstSCI CCNGG 3 cut(s) 382, 569, 747
BstXI CCANNNNNNTGG 1 cut(s) 755
Bsu36I CCTNAGG 1 cut(s) 524
BsuRI GGCC 5 cut(s) 33, 382, 705, 768, 821
BtgI CCRYGG 1 cut(s) 286
BtgZI GCGATG 1 cut(s) 999
BtsCI GGATG 1 cut(s) 666
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 3 cut(s) 302, 660, 903
Cac8I GCNNGC 1 cut(s) 253
CciI TCATGA 1 cut(s) 814
Cfr13I GGNCC 4 cut(s) 32, 199, 573, 965
CseI GACGC 1 cut(s) 962
CsiI ACCWGGT 1 cut(s) 747
CviAII CATG 6 cut(s) 187, 287, 463, 547, 678, 815
DdeI CTNAG 3 cut(s) 524, 857, 980
DpnI GATC 6 cut(s) 42, 101, 132, 432, 813, 993
DpnII GATC 6 cut(s) 40, 99, 130, 430, 811, 991
Eam1104I CTCTTC 3 cut(s) 351, 612, 621
EarI CTCTTC 3 cut(s) 351, 612, 621
Ecl136II GAGCTC 1 cut(s) 511
Eco130I CCWWGG 4 cut(s) 286, 351, 534, 1077
Eco147I AGGCCT 2 cut(s) 768, 821
Eco24I GRGCYC 1 cut(s) 513
Eco47I GGWCC 3 cut(s) 199, 573, 965
Eco53kI GAGCTC 1 cut(s) 511
Eco57I CTGAAG 1 cut(s) 728
Eco81I CCTNAGG 1 cut(s) 524
Eco88I CYCGRG 1 cut(s) 1037
EcoICRI GAGCTC 1 cut(s) 511
EcoRII CCWGG 2 cut(s) 382, 747
EcoT14I CCWWGG 4 cut(s) 286, 351, 534, 1077
EcoT38I GRGCYC 1 cut(s) 513
ErhI CCWWGG 4 cut(s) 286, 351, 534, 1077
FaeI CATG 6 cut(s) 190, 290, 466, 550, 681, 818
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FaqI GGGAC 2 cut(s) 92, 169
FatI CATG 6 cut(s) 186, 286, 462, 546, 677, 814
FbaI TGATCA 3 cut(s) 40, 811, 991
Fnu4HI GCNGC 1 cut(s) 730
FokI GGATG 1 cut(s) 673
FriOI GRGCYC 1 cut(s) 513
Fsp4HI GCNGC 1 cut(s) 730
FspBI CTAG 2 cut(s) 695, 848
GluI GCNGC 1 cut(s) 730
HaeIII GGCC 5 cut(s) 33, 382, 705, 768, 821
HapII CCGG 1 cut(s) 571
HgaI GACGC 1 cut(s) 962
Hin1II CATG 6 cut(s) 190, 290, 466, 550, 681, 818
HincII GTYRAC 2 cut(s) 283, 952
HindII GTYRAC 2 cut(s) 283, 952
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 5 cut(s) 57, 92, 137, 664, 853
HpaI GTTAAC 1 cut(s) 283
HpaII CCGG 1 cut(s) 571
HphI GGTGA 3 cut(s) 73, 109, 398
Hpy166II GTNNAC 4 cut(s) 283, 468, 901, 952
Hpy188I TCNGA 7 cut(s) 151, 427, 721, 858, 907, 981, 996
Hpy188III TCNNGA 7 cut(s) 54, 134, 526, 815, 848, 1037, 1039
Hpy8I GTNNAC 4 cut(s) 283, 468, 901, 952
HpyAV CCTTC 3 cut(s) 395, 480, 779
HpyCH4III ACNGT 2 cut(s) 415, 898
HpyCH4V TGCA 5 cut(s) 218, 244, 255, 302, 649
HpyF3I CTNAG 3 cut(s) 524, 857, 980
Hsp92II CATG 6 cut(s) 190, 290, 466, 550, 681, 818
Ksp22I TGATCA 3 cut(s) 40, 811, 991
KspAI GTTAAC 1 cut(s) 283
Kzo9I GATC 6 cut(s) 40, 99, 130, 430, 811, 991
LmnI GCTCC 2 cut(s) 516, 1081
LweI GCATC 2 cut(s) 741, 976
MabI ACCWGGT 1 cut(s) 747
MaeI CTAG 2 cut(s) 695, 848
MaeIII GTNAC 3 cut(s) 449, 892, 970
MalI GATC 6 cut(s) 42, 101, 132, 432, 813, 993
MboI GATC 6 cut(s) 40, 99, 130, 430, 811, 991
MboII GAAGA 8 cut(s) 368, 440, 629, 638, 650, 928, 999, 1039
MfeI CAATTG 2 cut(s) 194, 924
MhlI GDGCHC 2 cut(s) 513, 888
MlyI GAGTC 3 cut(s) 66, 146, 658
MmeI TCCRAC 1 cut(s) 531
MnlI CCTC 7 cut(s) 64, 120, 325, 533, 651, 723, 811
MroXI GAANNNNTTC 2 cut(s) 1032, 1046
MseI TTAA 6 cut(s) 282, 318, 753, 777, 1062, 1099
MslI CAYNNNNRTG 2 cut(s) 185, 720
MspI CCGG 1 cut(s) 571
MspR9I CCNGG 3 cut(s) 384, 571, 749
MunI CAATTG 2 cut(s) 194, 924
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 2 cut(s) 384, 749
NciI CCSGG 1 cut(s) 571
NcoI CCATGG 1 cut(s) 286
NdeII GATC 6 cut(s) 40, 99, 130, 430, 811, 991
NlaIII CATG 6 cut(s) 190, 290, 466, 550, 681, 818
NlaIV GGNNCC 4 cut(s) 34, 412, 575, 797
NmeAIII GCCGAG 1 cut(s) 227
NmuCI GTSAC 1 cut(s) 970
NspI RCATGY 1 cut(s) 550
PaeR7I CTCGAG 1 cut(s) 1037
PagI TCATGA 1 cut(s) 814
PceI AGGCCT 2 cut(s) 768, 821
PciI ACATGT 1 cut(s) 546
PctI GAATGC 1 cut(s) 535
PdmI GAANNNNTTC 2 cut(s) 1032, 1046
PfeI GAWTC 2 cut(s) 92, 853
PflMI CCANNNNNTGG 1 cut(s) 1010
PkrI GCNGC 1 cut(s) 731
PleI GAGTC 3 cut(s) 65, 145, 658
PpsI GAGTC 3 cut(s) 65, 145, 658
PscI ACATGT 1 cut(s) 546
Psp124BI GAGCTC 1 cut(s) 513
Psp6I CCWGG 2 cut(s) 382, 747
PspGI CCWGG 2 cut(s) 382, 747
PspN4I GGNNCC 4 cut(s) 34, 412, 575, 797
PspPI GGNCC 4 cut(s) 32, 199, 573, 965
RseI CAYNNNNRTG 2 cut(s) 185, 720
SacI GAGCTC 1 cut(s) 513
SaqAI TTAA 6 cut(s) 282, 318, 753, 777, 1062, 1099
SatI GCNGC 1 cut(s) 730
Sau3AI GATC 6 cut(s) 40, 99, 130, 430, 811, 991
Sau96I GGNCC 4 cut(s) 32, 199, 573, 965
SchI GAGTC 3 cut(s) 66, 146, 658
ScrFI CCNGG 3 cut(s) 384, 571, 749
SduI GDGCHC 2 cut(s) 513, 888
SexAI ACCWGGT 1 cut(s) 747
SfaNI GCATC 2 cut(s) 741, 976
Sfr274I CTCGAG 1 cut(s) 1037
SinI GGWCC 3 cut(s) 199, 573, 965
SlaI CTCGAG 1 cut(s) 1037
SmiMI CAYNNNNRTG 2 cut(s) 185, 720
SmlI CTYRAG 2 cut(s) 233, 1037
SmoI CTYRAG 2 cut(s) 233, 1037
SseBI AGGCCT 2 cut(s) 768, 821
SsiI CCGC 1 cut(s) 729
SspI AATATT 1 cut(s) 835
SspMI CTAG 2 cut(s) 695, 848
SstI GAGCTC 1 cut(s) 513
StuI AGGCCT 2 cut(s) 768, 821
StyD4I CCNGG 3 cut(s) 382, 569, 747
StyI CCWWGG 4 cut(s) 286, 351, 534, 1077
TaaI ACNGT 2 cut(s) 415, 898
TaqI TCGA 2 cut(s) 441, 1038
TauI GCSGC 1 cut(s) 732
TfiI GAWTC 2 cut(s) 92, 853
Tru1I TTAA 6 cut(s) 282, 318, 753, 777, 1062, 1099
Tru9I TTAA 6 cut(s) 282, 318, 753, 777, 1062, 1099
TscAI CASTG 3 cut(s) 309, 660, 903
TseFI GTSAC 1 cut(s) 970
Tsp45I GTSAC 1 cut(s) 970
TspDTI ATGAA 2 cut(s) 451, 623
TspRI CASTG 3 cut(s) 309, 660, 903
Van91I CCANNNNNTGG 1 cut(s) 1010
VpaK11BI GGWCC 3 cut(s) 199, 573, 965
XapI RAATTY 2 cut(s) 267, 1031
XbaI TCTAGA 1 cut(s) 847
XceI RCATGY 1 cut(s) 550
XcmI CCANNNNNNNNNTGG 1 cut(s) 752
XhoI CTCGAG 1 cut(s) 1037
XmnI GAANNNNTTC 2 cut(s) 1032, 1046
XspI CTAG 2 cut(s) 695, 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.