MD05G1292700.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001297
Physical Location & Seq
Forward (+)
42419984 .. 42422615
2632 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1292700.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGGCATCACTATCGCAGCTCCCAGCAGGCTCTGGAAGCTCTAATTCGCTACTCAGCGTCATAGAGACCGCCAAAGAGCCCTTAACTTCAGTCCCACAATGTTACGTTCGTTTGCAACAAGAACCAACTCCATCTGAACTCTCTGATGATAATAAGGGCACCTTCCGTTCTATCCCTACCATTGACATGACTAAATTAGTTAAGGGAGAAACCTCAGACTTTGCAAATCAACTGGAGAAACTGCACTCAGCTTGCAAAGATTTGGGCATTTTTCAGTTGGTGAACCATGGAGTTAACCCTATGCTGTTAGAGAATCTGGAACATGAGATTGAAGTATTCTTTAAGCTTCCCTTGGAAGAGAAAATGAAATATGCAATAAGGTCAGGTGATGTTGAAGGCTATGGAAACATAGCCAAGACCAAAGACCAAAAGCTTGACTGGGGTGATAGGTTCTATATGATAACCAACCCTATTCATCGAAGAAAGCCGTACCTCTTCGCAGAGCTCCCTTCATCCATCAGGAATACCTTGGAGTCATACTTTATGGAATTGGACCAACTTGCCATGAGACTTCTTGGGTTTATGGTGAAAGCTCTGAAGACAGATATGAGAGAGATAGAGGAGTTGTTTGATACAGACGGGATGCAATCAGTGAGGATGACATACTATCCTCCATGTCCACAACCAGAGCTGGTTGTAGGGCTCTCACCTCACTCAGATGTATGTGGGATCACCATCCTCAACCAGCTTAATGGAGTTGACGGTCTCCAAATTAAAAAAGACGGGGCTTGGATGCCTGTCAACTTCCAAAAAGATGCCTTTGTGGTGAATGTCGGGGACATTTTTGAGATGCTGAGCAACGGAGTTTACAAAAGCATTGAACACAGGGCAATGGTGAATTCAGAGAAAGAAAGGGTGTCGGTTGCGATGTTCTTCAGCCCTAAATTTGAGGCAGAGATTGGGCCACTCAGAAGCTTGATAACCCCAGAAAACCCACCACTGTTCCAAGGGGTTGGGATGGAAAAGTACGTCAACGATTTCTTCTCCCGCCTTAGGCTTGACGGGAAATCGTACTTGGAGCAAATGAAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

365

Amino Acids

41.19

Weight (kDa)

5.27

Isoelectric Point (pI)

43.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 58 - 157 5.8e-26 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 216 - 314 1.7e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 158
AciI CCGC 2 cut(s) 69, 1048
AclWI GGATC 1 cut(s) 737
AcsI RAATTY 2 cut(s) 898, 944
AcuI CTGAAG 3 cut(s) 72, 617, 919
AfaI GTAC 3 cut(s) 491, 1028, 1073
AfiI CCNNNNNNNGG 1 cut(s) 1053
AgsI TTSAA 3 cut(s) 332, 395, 881
AjuI GAANNNNNNNTTGG 2 cut(s) 1058, 1090
Alw21I GWGCWC 1 cut(s) 507
Alw26I GTCTC 3 cut(s) 59, 562, 770
AlwI GGATC 1 cut(s) 737
AlwNI CAGNNNCTG 1 cut(s) 32
AoxI GGCC 1 cut(s) 962
ApeKI GCWGC 1 cut(s) 16
ApoI RAATTY 2 cut(s) 898, 944
AspS9I GGNCC 2 cut(s) 553, 962
AsuHPI GGTGA 8 cut(s) 292, 398, 455, 598, 699, 724, 838, 907
AvaII GGWCC 1 cut(s) 553
AxyI CCTNAGG 1 cut(s) 1052
BaeGI GKGCMC 1 cut(s) 161
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 3 cut(s) 81, 507, 705
BbsI GAAGAC 1 cut(s) 605
Bbv12I GWGCWC 1 cut(s) 507
BbvI GCAGC 1 cut(s) 28
BccI CCATC 4 cut(s) 139, 524, 743, 1012
BceAI ACGGC 1 cut(s) 472
BcgI CGANNNNNNTGC 1 cut(s) 28
BcoDI GTCTC 3 cut(s) 59, 562, 770
BisI GCNGC 1 cut(s) 17
BlpI GCTNAGC 1 cut(s) 854
BlsI GCNGC 1 cut(s) 18
Bme18I GGWCC 1 cut(s) 553
BmgT120I GGNCC 2 cut(s) 553, 962
BmiI GGNNCC 1 cut(s) 160
BmrI ACTGGG 1 cut(s) 448
BmsI GCATC 5 cut(s) 14, 633, 783, 805, 840
BmuI ACTGGG 1 cut(s) 448
BpiI GAAGAC 1 cut(s) 605
BpmI CTGGAG 1 cut(s) 254
Bpu1102I GCTNAGC 1 cut(s) 854
BsaBI GATNNNNATC 1 cut(s) 734
BsaI GGTCTC 2 cut(s) 59, 770
BsaJI CCNNGG 4 cut(s) 286, 351, 528, 1006
Bsc4I CCNNNNNNNGG 1 cut(s) 1053
Bse1I ACTGG 2 cut(s) 237, 443
Bse21I CCTNAGG 1 cut(s) 1052
Bse3DI GCAATG 1 cut(s) 897
Bse8I GATNNNNATC 1 cut(s) 734
BseDI CCNNGG 4 cut(s) 286, 351, 528, 1006
BseGI GGATG 6 cut(s) 512, 648, 663, 735, 798, 1023
BseJI GATNNNNATC 1 cut(s) 734
BseLI CCNNNNNNNGG 1 cut(s) 1053
BseMI GCAATG 1 cut(s) 897
BseMII CTCAG 6 cut(s) 67, 228, 261, 729, 845, 982
BseNI ACTGG 2 cut(s) 237, 443
BseRI GAGGAG 1 cut(s) 635
BseSI GKGCMC 1 cut(s) 161
BseXI GCAGC 1 cut(s) 28
BseYI CCCAGC 1 cut(s) 22
BsgI GTGCAG 1 cut(s) 227
BshFI GGCC 1 cut(s) 964
BshNI GGYRCC 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 507
BslFI GGGAC 2 cut(s) 77, 851
BslI CCNNNNNNNGG 1 cut(s) 1053
BsmAI GTCTC 3 cut(s) 59, 562, 770
BsmFI GGGAC 2 cut(s) 77, 851
BsnI GGCC 1 cut(s) 964
Bso31I GGTCTC 2 cut(s) 59, 770
Bsp1286I GDGCHC 4 cut(s) 81, 161, 507, 705
Bsp143I GATC 1 cut(s) 729
Bsp1720I GCTNAGC 1 cut(s) 854
Bsp19I CCATGG 1 cut(s) 286
BspACI CCGC 2 cut(s) 69, 1048
BspANI GGCC 1 cut(s) 964
BspCNI CTCAG 6 cut(s) 66, 227, 260, 728, 846, 981
BspLI GGNNCC 1 cut(s) 160
BspPI GGATC 1 cut(s) 737
BspT107I GGYRCC 1 cut(s) 158
BspTNI GGTCTC 2 cut(s) 59, 770
BsrDI GCAATG 1 cut(s) 897
BsrI ACTGG 2 cut(s) 237, 443
BssECI CCNNGG 4 cut(s) 286, 351, 528, 1006
BssMI GATC 1 cut(s) 729
BssT1I CCWWGG 4 cut(s) 286, 351, 528, 1006
Bst4CI ACNGT 2 cut(s) 764, 1002
Bst6I CTCTTC 2 cut(s) 351, 500
BstC8I GCNNGC 2 cut(s) 28, 253
BstDEI CTNAG 7 cut(s) 53, 214, 247, 715, 854, 968, 1052
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 6 cut(s) 512, 648, 663, 735, 798, 1023
BstKTI GATC 1 cut(s) 732
BstMAI GTCTC 3 cut(s) 59, 562, 770
BstMBI GATC 1 cut(s) 729
BstMWI GCNNNNNNNGC 1 cut(s) 36
BstSLI GKGCMC 1 cut(s) 161
BstV1I GCAGC 1 cut(s) 28
BstV2I GAAGAC 1 cut(s) 605
BstXI CCANNNNNNTGG 2 cut(s) 752, 1013
Bsu36I CCTNAGG 1 cut(s) 1052
BsuRI GGCC 1 cut(s) 964
BtgI CCRYGG 1 cut(s) 286
BtgZI GCGATG 1 cut(s) 941
BtsCI GGATG 6 cut(s) 512, 648, 663, 735, 798, 1023
BtsIMutI CAGTG 2 cut(s) 657, 998
Cac8I GCNNGC 2 cut(s) 28, 253
CaiI CAGNNNCTG 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 553, 962
CseI GACGC 1 cut(s) 46
Csp6I GTAC 3 cut(s) 490, 1027, 1072
CviAII CATG 5 cut(s) 187, 287, 323, 565, 675
CviQI GTAC 3 cut(s) 490, 1027, 1072
DdeI CTNAG 7 cut(s) 53, 214, 247, 715, 854, 968, 1052
DpnI GATC 1 cut(s) 731
DpnII GATC 1 cut(s) 729
Eam1104I CTCTTC 2 cut(s) 351, 500
EarI CTCTTC 2 cut(s) 351, 500
Ecl136II GAGCTC 1 cut(s) 505
Eco130I CCWWGG 4 cut(s) 286, 351, 528, 1006
Eco24I GRGCYC 3 cut(s) 81, 507, 705
Eco31I GGTCTC 2 cut(s) 59, 770
Eco47I GGWCC 1 cut(s) 553
Eco53kI GAGCTC 1 cut(s) 505
Eco57I CTGAAG 3 cut(s) 72, 617, 919
Eco81I CCTNAGG 1 cut(s) 1052
EcoICRI GAGCTC 1 cut(s) 505
EcoRI GAATTC 1 cut(s) 898
EcoT14I CCWWGG 4 cut(s) 286, 351, 528, 1006
EcoT38I GRGCYC 3 cut(s) 81, 507, 705
ErhI CCWWGG 4 cut(s) 286, 351, 528, 1006
FaeI CATG 5 cut(s) 190, 290, 326, 568, 678
FalI AAGNNNNNCTT 4 cut(s) 146, 178, 335, 367
FaqI GGGAC 2 cut(s) 77, 851
FatI CATG 5 cut(s) 186, 286, 322, 564, 674
FauI CCCGC 1 cut(s) 1055
Fnu4HI GCNGC 1 cut(s) 17
FokI GGATG 6 cut(s) 499, 655, 670, 722, 805, 1030
FriOI GRGCYC 3 cut(s) 81, 507, 705
Fsp4HI GCNGC 1 cut(s) 17
GluI GCNGC 1 cut(s) 17
GsaI CCCAGC 1 cut(s) 26
GsuI CTGGAG 1 cut(s) 254
HaeIII GGCC 1 cut(s) 964
HgaI GACGC 1 cut(s) 46
Hin1II CATG 5 cut(s) 190, 290, 326, 568, 678
HincII GTYRAC 4 cut(s) 295, 760, 802, 1033
HindII GTYRAC 4 cut(s) 295, 760, 802, 1033
HindIII AAGCTT 3 cut(s) 344, 431, 973
HinfI GANTC 2 cut(s) 313, 533
HpaI GTTAAC 1 cut(s) 295
HphI GGTGA 8 cut(s) 292, 398, 455, 598, 699, 724, 838, 907
Hpy166II GTNNAC 7 cut(s) 283, 295, 680, 760, 802, 868, 1033
Hpy188I TCNGA 7 cut(s) 136, 145, 217, 597, 718, 904, 971
Hpy188III TCNNGA 3 cut(s) 33, 317, 520
Hpy8I GTNNAC 7 cut(s) 283, 295, 680, 760, 802, 868, 1033
HpyAV CCTTC 3 cut(s) 172, 389, 519
HpyCH4III ACNGT 2 cut(s) 764, 1002
HpyCH4IV ACGT 2 cut(s) 105, 1029
HpyCH4V TGCA 6 cut(s) 115, 224, 244, 255, 374, 646
HpyF10VI GCNNNNNNNGC 1 cut(s) 36
HpyF3I CTNAG 7 cut(s) 53, 214, 247, 715, 854, 968, 1052
HpySE526I ACGT 2 cut(s) 105, 1029
Hsp92II CATG 5 cut(s) 190, 290, 326, 568, 678
KspAI GTTAAC 1 cut(s) 295
Kzo9I GATC 1 cut(s) 729
LmnI GCTCC 3 cut(s) 24, 510, 1078
Lsp1109I GCAGC 1 cut(s) 28
LweI GCATC 5 cut(s) 14, 633, 783, 805, 840
MaeII ACGT 2 cut(s) 105, 1029
MaeIII GTNAC 1 cut(s) 101
MalI GATC 1 cut(s) 731
MboI GATC 1 cut(s) 729
MboII GAAGA 6 cut(s) 368, 487, 492, 610, 925, 1033
MhlI GDGCHC 4 cut(s) 81, 161, 507, 705
MluCI AATT 6 cut(s) 43, 194, 548, 771, 898, 944
MlyI GAGTC 1 cut(s) 542
MnlI CCTC 8 cut(s) 223, 503, 613, 648, 681, 720, 749, 943
MseI TTAA 6 cut(s) 83, 201, 294, 342, 750, 774
MslI CAYNNNNRTG 2 cut(s) 185, 717
MwoI GCNNNNNNNGC 1 cut(s) 36
NcoI CCATGG 1 cut(s) 286
NdeII GATC 1 cut(s) 729
NlaIII CATG 5 cut(s) 190, 290, 326, 568, 678
NlaIV GGNNCC 1 cut(s) 160
PfeI GAWTC 1 cut(s) 313
PkrI GCNGC 1 cut(s) 18
PleI GAGTC 1 cut(s) 541
PpsI GAGTC 1 cut(s) 541
Psp124BI GAGCTC 1 cut(s) 507
PspFI CCCAGC 1 cut(s) 22
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 2 cut(s) 553, 962
PstNI CAGNNNCTG 1 cut(s) 32
RsaI GTAC 3 cut(s) 491, 1028, 1073
RsaNI GTAC 3 cut(s) 490, 1027, 1072
RseI CAYNNNNRTG 2 cut(s) 185, 717
SacI GAGCTC 1 cut(s) 507
SaqAI TTAA 6 cut(s) 83, 201, 294, 342, 750, 774
SatI GCNGC 1 cut(s) 17
Sau3AI GATC 1 cut(s) 729
Sau96I GGNCC 2 cut(s) 553, 962
SchI GAGTC 1 cut(s) 542
SduI GDGCHC 4 cut(s) 81, 161, 507, 705
SfaNI GCATC 5 cut(s) 14, 633, 783, 805, 840
SinI GGWCC 1 cut(s) 553
SmiMI CAYNNNNRTG 2 cut(s) 185, 717
Sse9I AATT 6 cut(s) 43, 194, 548, 771, 898, 944
SsiI CCGC 2 cut(s) 69, 1048
SstI GAGCTC 1 cut(s) 507
StyI CCWWGG 4 cut(s) 286, 351, 528, 1006
TaaI ACNGT 2 cut(s) 764, 1002
TaiI ACGT 2 cut(s) 108, 1032
TaqI TCGA 1 cut(s) 478
TasI AATT 6 cut(s) 43, 194, 548, 771, 898, 944
TfiI GAWTC 1 cut(s) 313
Tru1I TTAA 6 cut(s) 83, 201, 294, 342, 750, 774
Tru9I TTAA 6 cut(s) 83, 201, 294, 342, 750, 774
TscAI CASTG 2 cut(s) 657, 1005
TseI GCWGC 1 cut(s) 16
TspDTI ATGAA 3 cut(s) 380, 464, 501
TspGWI ACGGA 2 cut(s) 155, 876
TspRI CASTG 2 cut(s) 657, 1005
VpaK11BI GGWCC 1 cut(s) 553
XapI RAATTY 2 cut(s) 898, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.