Rroxscaffold_2G00112310

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001297
Physical Location & Seq
Forward (+)
37350173 .. 37352133
1961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00112310.1

Sequence Viewer

Length: 600 bp
ATGAGATACAAAGTAAGGCCAGGTGATGTTGAAGGCTATGGAACTGTGGTCAAATCCCAAGATCAAAAACTCGACTGGGGTAACAGGAATACCTTAGAGACATACATGTTGGAATTACAAAGGATTGCCCGGACCCTTTTAGGGTTGATGGGAAAACTTCTAAAGATGAAGATGGAGGAGATGGAAAAGTTGTTTGAAGATGGTCTGCAGTCACTGAGGATGACTCACTATCCTCCATGCCCAAAACCAGAACTAGTTATTGGCCTCAGGCCTCATTCTGATGGTAGTGGCATCACTATTCTCCACCAGGTTGGTGGAGTTCAAGGCCTTCAAATTAAAAAAGATGGGGCTTGGTTCCCTGTGATCTTTGACCATGGTGCCTTTGTTGTCAATGTTGGAGACATTCTAGAGATTCTGAGTGATGGGCTTTATAAAAGTATCGAGCATAGAGTGAATGTGAACCCAGAGAAAGAAAGGATTTCAATTGCTATGTTCTTCAAGCCCAAAGTTGAGGCAAAAATTGGACCTATGACCGCCCTGAGAGACCCTCCATTTTCCTCTCCTCAAAAAGACATGTTGTCTATTAGTTGTGTAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

199

Amino Acids

22.42

Weight (kDa)

8.47

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 70 - 168 5.9e-31 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 432
AccB1I GGYRCC 1 cut(s) 377
AciI CCGC 1 cut(s) 534
AfiI CCNNNNNNNGG 1 cut(s) 141
AflIII ACRYGT 2 cut(s) 105, 573
AgsI TTSAA 6 cut(s) 32, 197, 323, 332, 483, 499
AhdI GACNNNNNGTC 1 cut(s) 577
AhlI ACTAGT 1 cut(s) 253
AjnI CCWGG 2 cut(s) 19, 306
AjuI GAANNNNNNNTTGG 2 cut(s) 243, 275
Alw26I GTCTC 3 cut(s) 92, 393, 537
AlwNI CAGNNNCTG 1 cut(s) 214
AoxI GGCC 4 cut(s) 17, 262, 269, 325
AspS9I GGNCC 2 cut(s) 132, 524
AsuC2I CCSGG 1 cut(s) 130
AsuHPI GGTGA 1 cut(s) 35
AvaII GGWCC 2 cut(s) 132, 524
AxyI CCTNAGG 1 cut(s) 266
BanI GGYRCC 1 cut(s) 377
BccI CCATC 7 cut(s) 142, 166, 175, 194, 275, 338, 416
BciT130I CCWGG 2 cut(s) 21, 308
BcnI CCSGG 1 cut(s) 130
BcoDI GTCTC 3 cut(s) 92, 393, 537
BcuI ACTAGT 1 cut(s) 253
BfaI CTAG 2 cut(s) 254, 407
BfmI CTRYAG 1 cut(s) 206
Bme1390I CCNGG 3 cut(s) 21, 130, 308
Bme18I GGWCC 2 cut(s) 132, 524
BmeRI GACNNNNNGTC 1 cut(s) 577
BmgT120I GGNCC 2 cut(s) 132, 524
BmiI GGNNCC 3 cut(s) 134, 356, 379
BmrFI CCNGG 3 cut(s) 21, 130, 308
BmrI ACTGGG 1 cut(s) 85
BmsI GCATC 1 cut(s) 300
BmuI ACTGGG 1 cut(s) 85
BplI GAGNNNNNCTC 4 cut(s) 208, 240, 532, 564
BpuMI CCSGG 1 cut(s) 130
BsaI GGTCTC 1 cut(s) 537
BsaJI CCNNGG 1 cut(s) 373
Bsc4I CCNNNNNNNGG 1 cut(s) 141
Bse1I ACTGG 1 cut(s) 80
Bse21I CCTNAGG 1 cut(s) 266
BseBI CCWGG 2 cut(s) 21, 308
BseDI CCNNGG 1 cut(s) 373
BseGI GGATG 1 cut(s) 225
BseLI CCNNNNNNNGG 1 cut(s) 141
BseMII CTCAG 4 cut(s) 206, 280, 407, 530
BseNI ACTGG 1 cut(s) 80
BseRI GAGGAG 2 cut(s) 191, 552
BshFI GGCC 4 cut(s) 19, 264, 271, 327
BshNI GGYRCC 1 cut(s) 377
BsiSI CCGG 1 cut(s) 130
BslI CCNNNNNNNGG 1 cut(s) 141
BsmAI GTCTC 3 cut(s) 92, 393, 537
BsnI GGCC 4 cut(s) 19, 264, 271, 327
Bso31I GGTCTC 1 cut(s) 537
Bsp143I GATC 2 cut(s) 61, 363
Bsp19I CCATGG 1 cut(s) 373
BspACI CCGC 1 cut(s) 534
BspANI GGCC 4 cut(s) 19, 264, 271, 327
BspCNI CTCAG 4 cut(s) 207, 279, 408, 531
BspLI GGNNCC 3 cut(s) 134, 356, 379
BspMAI CTGCAG 1 cut(s) 210
BspT107I GGYRCC 1 cut(s) 377
BspTNI GGTCTC 1 cut(s) 537
BsrI ACTGG 1 cut(s) 80
BssECI CCNNGG 1 cut(s) 373
BssMI GATC 2 cut(s) 61, 363
BssT1I CCWWGG 1 cut(s) 373
Bst2UI CCWGG 2 cut(s) 21, 308
Bst4CI ACNGT 1 cut(s) 46
BstDEI CTNAG 5 cut(s) 94, 215, 266, 416, 539
BstDSI CCRYGG 1 cut(s) 373
BstF5I GGATG 1 cut(s) 225
BstKTI GATC 2 cut(s) 64, 366
BstMAI GTCTC 3 cut(s) 92, 393, 537
BstMBI GATC 2 cut(s) 61, 363
BstNI CCWGG 2 cut(s) 21, 308
BstNSI RCATGY 2 cut(s) 109, 577
BstSCI CCNGG 3 cut(s) 19, 128, 306
BstSFI CTRYAG 1 cut(s) 206
BstXI CCANNNNNNTGG 2 cut(s) 311, 314
Bsu36I CCTNAGG 1 cut(s) 266
BsuRI GGCC 4 cut(s) 19, 264, 271, 327
BtgI CCRYGG 1 cut(s) 373
BtsCI GGATG 1 cut(s) 225
BtsIMutI CAGTG 1 cut(s) 212
CaiI CAGNNNCTG 1 cut(s) 214
Cfr13I GGNCC 2 cut(s) 132, 524
CsiI ACCWGGT 1 cut(s) 306
CviAII CATG 4 cut(s) 106, 237, 374, 574
CviJI RGCY 8 cut(s) 19, 36, 264, 271, 327, 350, 427, 502
CviKI_1 RGCY 8 cut(s) 19, 36, 264, 271, 327, 350, 427, 502
DdeI CTNAG 5 cut(s) 94, 215, 266, 416, 539
DpnI GATC 2 cut(s) 63, 365
DpnII GATC 2 cut(s) 61, 363
DriI GACNNNNNGTC 1 cut(s) 577
Eam1105I GACNNNNNGTC 1 cut(s) 577
Eco130I CCWWGG 1 cut(s) 373
Eco147I AGGCCT 2 cut(s) 271, 327
Eco31I GGTCTC 1 cut(s) 537
Eco47I GGWCC 2 cut(s) 132, 524
Eco81I CCTNAGG 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 19, 306
EcoT14I CCWWGG 1 cut(s) 373
ErhI CCWWGG 1 cut(s) 373
FaeI CATG 4 cut(s) 109, 240, 377, 577
FatI CATG 4 cut(s) 105, 236, 373, 573
FokI GGATG 1 cut(s) 232
FspBI CTAG 2 cut(s) 254, 407
HaeIII GGCC 4 cut(s) 19, 264, 271, 327
HapII CCGG 1 cut(s) 130
Hin1II CATG 4 cut(s) 109, 240, 377, 577
HinfI GANTC 2 cut(s) 223, 412
HpaII CCGG 1 cut(s) 130
HphI GGTGA 1 cut(s) 35
Hpy166II GTNNAC 1 cut(s) 460
Hpy188I TCNGA 2 cut(s) 280, 417
Hpy188III TCNNGA 1 cut(s) 407
Hpy8I GTNNAC 1 cut(s) 460
HpyAV CCTTC 2 cut(s) 26, 338
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4V TGCA 1 cut(s) 208
HpyF3I CTNAG 5 cut(s) 94, 215, 266, 416, 539
Hsp92II CATG 4 cut(s) 109, 240, 377, 577
Kzo9I GATC 2 cut(s) 61, 363
LweI GCATC 1 cut(s) 300
MabI ACCWGGT 1 cut(s) 306
MaeI CTAG 2 cut(s) 254, 407
MaeIII GTNAC 2 cut(s) 80, 210
MalI GATC 2 cut(s) 63, 365
MboI GATC 2 cut(s) 61, 363
MboII GAAGA 3 cut(s) 181, 209, 487
MfeI CAATTG 1 cut(s) 483
MluCI AATT 4 cut(s) 113, 333, 483, 519
MlyI GAGTC 1 cut(s) 217
MmeI TCCRAC 2 cut(s) 90, 376
MnlI CCTC 9 cut(s) 169, 210, 243, 275, 282, 505, 558, 568, 573
MseI TTAA 1 cut(s) 336
MslI CAYNNNNRTG 1 cut(s) 279
MspI CCGG 1 cut(s) 130
MspR9I CCNGG 3 cut(s) 21, 130, 308
MunI CAATTG 1 cut(s) 483
MvaI CCWGG 2 cut(s) 21, 308
NciI CCSGG 1 cut(s) 130
NcoI CCATGG 1 cut(s) 373
NdeII GATC 2 cut(s) 61, 363
NlaIII CATG 4 cut(s) 109, 240, 377, 577
NlaIV GGNNCC 3 cut(s) 134, 356, 379
NmuCI GTSAC 1 cut(s) 210
NspI RCATGY 2 cut(s) 109, 577
PceI AGGCCT 2 cut(s) 271, 327
PciI ACATGT 2 cut(s) 105, 573
PfeI GAWTC 1 cut(s) 412
PleI GAGTC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 217
PscI ACATGT 2 cut(s) 105, 573
PsiI TTATAA 1 cut(s) 432
Psp6I CCWGG 2 cut(s) 19, 306
PspGI CCWGG 2 cut(s) 19, 306
PspN4I GGNNCC 3 cut(s) 134, 356, 379
PspPI GGNCC 2 cut(s) 132, 524
PstI CTGCAG 1 cut(s) 210
PstNI CAGNNNCTG 1 cut(s) 214
RseI CAYNNNNRTG 1 cut(s) 279
SaqAI TTAA 1 cut(s) 336
Sau3AI GATC 2 cut(s) 61, 363
Sau96I GGNCC 2 cut(s) 132, 524
SchI GAGTC 1 cut(s) 217
ScrFI CCNGG 3 cut(s) 21, 130, 308
SetI ASST 4 cut(s) 25, 95, 312, 529
SexAI ACCWGGT 1 cut(s) 306
SfaNI GCATC 1 cut(s) 300
SfcI CTRYAG 1 cut(s) 206
SinI GGWCC 2 cut(s) 132, 524
SmiMI CAYNNNNRTG 1 cut(s) 279
SpeI ACTAGT 1 cut(s) 253
Sse9I AATT 4 cut(s) 113, 333, 483, 519
SseBI AGGCCT 2 cut(s) 271, 327
SsiI CCGC 1 cut(s) 534
SspMI CTAG 2 cut(s) 254, 407
StuI AGGCCT 2 cut(s) 271, 327
StyD4I CCNGG 3 cut(s) 19, 128, 306
StyI CCWWGG 1 cut(s) 373
TaaI ACNGT 1 cut(s) 46
TaqI TCGA 2 cut(s) 72, 441
TasI AATT 4 cut(s) 113, 333, 483, 519
TfiI GAWTC 1 cut(s) 412
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 1 cut(s) 219
TseFI GTSAC 1 cut(s) 210
Tsp45I GTSAC 1 cut(s) 210
TspDTI ATGAA 1 cut(s) 182
TspRI CASTG 1 cut(s) 219
VpaK11BI GGWCC 2 cut(s) 132, 524
XbaI TCTAGA 1 cut(s) 406
XceI RCATGY 2 cut(s) 109, 577
XcmI CCANNNNNNNNNTGG 1 cut(s) 311
XspI CTAG 2 cut(s) 254, 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.