RchiOBHm_Chr5g0012711

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001297
Physical Location & Seq
Forward (+)
8609386 .. 8613061
3676 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29326

Sequence Viewer

Length: 1095 bp
ATGGCATCACCCCAGCTACGGCTACCAGGAGGCTTTGAGAGCTCTAGTTCTCTTATGAGCTTCCTGGAGATCACCAAAGAGTCATTAACTGAAGTCCCACAATGTTACGTCCGTAGTTTCGAAGAACCAGAACTTTCATCTGATCTCTCTGATAAGCCCACCGTAATCCCAACCATCGATATGATAAAACTAGTAACGGGATCAGAAGCTTCAGATTCTGAACTGGAAAATCTGCGCTCAACTTGCAAAGAGTGGGGAATCTTTCAGTTGGTGAACCACGGAGTTAGCCCTATGCTTTTGGAGAAGCTGAAGTACGAGGTTGAAGAATTCTTTAAGCTTCCCTTGGAAGAGAAAATGAAGTATCAGATAAGGCCTGGTGACGTTGAAGGCTATGGATCCATAGCCCAAAATAAAGAGCAAAAACGTGACTGGGGTAATAGATTCTACATGACCATGAACCCTGTTCATAGGAGAAAGCCATACCTCTTCCCAGAGCTCCCTTCATCCCTCAGGAATACCTTCGAGTCATACTACTTGGAACTGAAGCAACTAGCCATGGAACTTATTGGGTTTCTGGGGAAGGCTGTGAAGATAGACATAAAAGAGACTGAGGAGATGTTTGAAGATGGGATGCAGTCAGTGAGGATGAATTACTATCCTTCATGCCCACAACCAGAGCTGGTCATCGGCCTCACACCTCACTCGGACGCTTCCGGGATCACCATCCTCCACCAGCTTAACGGGGTGGGTGGTCTACAGATTAAGAAAGATGGGGTTTGGGTTCCTGTCAACTTCCGTGAAGATGCCTTTGTGGTTAATGCAGGGGACATCTTTGAGATCCTCAGCAATGGAGTGTACAAGAGCATGGAGCACAGAGTTATGGTGAACTCTGAGCAAGAGAGGATCTCAATCGCTATGTTCTTCAACCCCAAATTTGAGGCAGAAATTGGACCAGTGACCAGCTTGATAACCCCAGAAAACCCACCACTGTTCAAAAGGATGGGGATGGAACAGTACGTCCAAGATTTCTACACCCGCCGTAAGCTCGATGGGAAATCATACTTGGACCAGATGAGGCTCCAGAATGAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

364

Amino Acids

41.78

Weight (kDa)

5.08

Isoelectric Point (pI)

51.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 56 - 156 6.6e-27 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 212 - 310 2.2e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 754
AciI CCGC 1 cut(s) 1036
AclWI GGATC 6 cut(s) 208, 390, 403, 725, 832, 911
AcsI RAATTY 2 cut(s) 326, 932
AcuI CTGAAG 4 cut(s) 111, 195, 329, 563
AfaI GTAC 3 cut(s) 314, 857, 1016
AfiI CCNNNNNNNGG 1 cut(s) 18
AgsI TTSAA 5 cut(s) 323, 386, 623, 925, 994
AhlI ACTAGT 1 cut(s) 190
AjnI CCWGG 3 cut(s) 25, 63, 373
AjuI GAANNNNNNNTTGG 2 cut(s) 1046, 1078
AloI GAACNNNNNNTCC 2 cut(s) 1002, 1034
Alw21I GWGCWC 3 cut(s) 44, 498, 873
Alw26I GTCTC 1 cut(s) 599
AlwI GGATC 6 cut(s) 208, 390, 403, 725, 832, 911
AlwNI CAGNNNCTG 1 cut(s) 218
AoxI GGCC 2 cut(s) 371, 688
ApoI RAATTY 2 cut(s) 326, 932
Asp700I GAANNNNTTC 1 cut(s) 518
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 2 cut(s) 950, 1066
AsuC2I CCSGG 1 cut(s) 715
AsuHPI GGTGA 5 cut(s) 64, 283, 389, 712, 895
AsuII TTCGAA 1 cut(s) 120
AvaII GGWCC 2 cut(s) 950, 1066
AxyI CCTNAGG 1 cut(s) 509
BamHI GGATCC 1 cut(s) 395
BanII GRGCYC 2 cut(s) 44, 498
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
Bbv12I GWGCWC 3 cut(s) 44, 498, 873
BbvCI CCTCAGC 1 cut(s) 842
BccI CCATC 7 cut(s) 182, 620, 731, 764, 994, 1000, 1043
BceAI ACGGC 2 cut(s) 35, 1023
BciT130I CCWGG 3 cut(s) 27, 65, 375
BcnI CCSGG 1 cut(s) 715
BcoDI GTCTC 1 cut(s) 599
BcuI ACTAGT 1 cut(s) 190
BfaI CTAG 3 cut(s) 45, 191, 551
BfmI CTRYAG 1 cut(s) 755
Bme1390I CCNGG 4 cut(s) 27, 65, 375, 715
Bme18I GGWCC 2 cut(s) 950, 1066
BmgT120I GGNCC 2 cut(s) 950, 1066
BmiI GGNNCC 3 cut(s) 397, 783, 1079
BmrFI CCNGG 4 cut(s) 27, 65, 375, 715
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 3 cut(s) 14, 621, 793
BmuI ACTGGG 1 cut(s) 439
BplI GAGNNNNNCTC 2 cut(s) 890, 922
BpmI CTGGAG 2 cut(s) 86, 1064
Bpu10I CCTNAGC 1 cut(s) 842
Bpu14I TTCGAA 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 715
Bsa29I ATCGAT 1 cut(s) 177
BsaBI GATNNNNATC 2 cut(s) 722, 908
BsaJI CCNNGG 3 cut(s) 277, 342, 555
Bsc4I CCNNNNNNNGG 1 cut(s) 18
Bse1I ACTGG 3 cut(s) 228, 434, 953
Bse21I CCTNAGG 1 cut(s) 509
Bse3DI GCAATG 1 cut(s) 853
Bse8I GATNNNNATC 2 cut(s) 722, 908
BseBI CCWGG 3 cut(s) 27, 65, 375
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 3 cut(s) 277, 342, 555
BseGI GGATG 6 cut(s) 503, 636, 651, 723, 1005, 1011
BseJI GATNNNNATC 2 cut(s) 722, 908
BseLI CCNNNNNNNGG 1 cut(s) 18
BseMI GCAATG 1 cut(s) 853
BseMII CTCAG 4 cut(s) 523, 600, 856, 882
BseNI ACTGG 3 cut(s) 228, 434, 953
BseRI GAGGAG 1 cut(s) 626
BseYI CCCAGC 1 cut(s) 12
BshFI GGCC 2 cut(s) 373, 690
BshVI ATCGAT 1 cut(s) 177
BsiHKAI GWGCWC 3 cut(s) 44, 498, 873
BsiSI CCGG 1 cut(s) 714
BslFI GGGAC 2 cut(s) 80, 839
BslI CCNNNNNNNGG 1 cut(s) 18
BsmAI GTCTC 1 cut(s) 599
BsmFI GGGAC 2 cut(s) 80, 839
BsnI GGCC 2 cut(s) 373, 690
Bsp119I TTCGAA 1 cut(s) 120
Bsp1286I GDGCHC 3 cut(s) 44, 498, 873
Bsp1407I TGTACA 1 cut(s) 855
Bsp143I GATC 7 cut(s) 69, 142, 200, 395, 717, 837, 903
Bsp19I CCATGG 1 cut(s) 555
BspACI CCGC 1 cut(s) 1036
BspANI GGCC 2 cut(s) 373, 690
BspCNI CTCAG 4 cut(s) 522, 601, 855, 883
BspDI ATCGAT 1 cut(s) 177
BspLI GGNNCC 3 cut(s) 397, 783, 1079
BspPI GGATC 6 cut(s) 208, 390, 403, 725, 832, 911
BspT104I TTCGAA 1 cut(s) 120
BsrDI GCAATG 1 cut(s) 853
BsrGI TGTACA 1 cut(s) 855
BsrI ACTGG 3 cut(s) 228, 434, 953
BssECI CCNNGG 3 cut(s) 277, 342, 555
BssMI GATC 7 cut(s) 69, 142, 200, 395, 717, 837, 903
BssT1I CCWWGG 2 cut(s) 342, 555
Bst2UI CCWGG 3 cut(s) 27, 65, 375
Bst4CI ACNGT 3 cut(s) 163, 990, 1014
Bst6I CTCTTC 2 cut(s) 342, 491
BstAUI TGTACA 1 cut(s) 855
BstBI TTCGAA 1 cut(s) 120
BstDEI CTNAG 4 cut(s) 509, 609, 842, 891
BstDSI CCRYGG 2 cut(s) 277, 555
BstF5I GGATG 6 cut(s) 503, 636, 651, 723, 1005, 1011
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 7 cut(s) 72, 145, 203, 398, 720, 840, 906
BstMAI GTCTC 1 cut(s) 599
BstMBI GATC 7 cut(s) 69, 142, 200, 395, 717, 837, 903
BstMWI GCNNNNNNNGC 2 cut(s) 39, 243
BstNI CCWGG 3 cut(s) 27, 65, 375
BstSCI CCNGG 4 cut(s) 25, 63, 373, 713
BstSFI CTRYAG 1 cut(s) 755
BstX2I RGATCY 3 cut(s) 395, 837, 903
BstYI RGATCY 3 cut(s) 395, 837, 903
Bsu15I ATCGAT 1 cut(s) 177
Bsu36I CCTNAGG 1 cut(s) 509
BsuRI GGCC 2 cut(s) 373, 690
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 2 cut(s) 277, 555
BtsCI GGATG 6 cut(s) 503, 636, 651, 723, 1005, 1011
BtsIMutI CAGTG 3 cut(s) 645, 960, 986
CaiI CAGNNNCTG 1 cut(s) 218
CfoI GCGC 1 cut(s) 237
Cfr13I GGNCC 2 cut(s) 950, 1066
ClaI ATCGAT 1 cut(s) 177
CseI GACGC 1 cut(s) 716
Csp6I GTAC 3 cut(s) 313, 856, 1015
CviAII CATG 5 cut(s) 448, 454, 556, 663, 865
CviQI GTAC 3 cut(s) 313, 856, 1015
DdeI CTNAG 4 cut(s) 509, 609, 842, 891
DpnI GATC 7 cut(s) 71, 144, 202, 397, 719, 839, 905
DpnII GATC 7 cut(s) 69, 142, 200, 395, 717, 837, 903
Eam1104I CTCTTC 2 cut(s) 342, 491
EarI CTCTTC 2 cut(s) 342, 491
Ecl136II GAGCTC 2 cut(s) 42, 496
Eco130I CCWWGG 2 cut(s) 342, 555
Eco147I AGGCCT 1 cut(s) 373
Eco24I GRGCYC 2 cut(s) 44, 498
Eco47I GGWCC 2 cut(s) 950, 1066
Eco53kI GAGCTC 2 cut(s) 42, 496
Eco57I CTGAAG 4 cut(s) 111, 195, 329, 563
Eco81I CCTNAGG 1 cut(s) 509
EcoICRI GAGCTC 2 cut(s) 42, 496
EcoRI GAATTC 1 cut(s) 326
EcoRII CCWGG 3 cut(s) 25, 63, 373
EcoT14I CCWWGG 2 cut(s) 342, 555
EcoT38I GRGCYC 2 cut(s) 44, 498
ErhI CCWWGG 2 cut(s) 342, 555
FaeI CATG 5 cut(s) 451, 457, 559, 666, 868
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FaqI GGGAC 2 cut(s) 80, 839
FatI CATG 5 cut(s) 447, 453, 555, 662, 864
FauI CCCGC 1 cut(s) 1043
FblI GTMKAC 1 cut(s) 754
FokI GGATG 6 cut(s) 490, 643, 658, 710, 1012, 1018
FriOI GRGCYC 2 cut(s) 44, 498
FspBI CTAG 3 cut(s) 45, 191, 551
GlaI GCGC 1 cut(s) 236
GsaI CCCAGC 1 cut(s) 16
GsuI CTGGAG 2 cut(s) 86, 1064
HaeIII GGCC 2 cut(s) 373, 690
HapII CCGG 1 cut(s) 714
HgaI GACGC 1 cut(s) 716
HhaI GCGC 1 cut(s) 237
Hin1II CATG 5 cut(s) 451, 457, 559, 666, 868
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HindIII AAGCTT 2 cut(s) 207, 335
HinfI GANTC 5 cut(s) 80, 215, 258, 441, 524
HpaII CCGG 1 cut(s) 714
HphI GGTGA 5 cut(s) 64, 283, 389, 712, 895
Hpy166II GTNNAC 5 cut(s) 274, 755, 790, 856, 886
Hpy188I TCNGA 8 cut(s) 142, 151, 205, 214, 220, 366, 706, 892
Hpy188III TCNNGA 2 cut(s) 511, 1081
Hpy8I GTNNAC 5 cut(s) 274, 755, 790, 856, 886
HpyAV CCTTC 5 cut(s) 380, 510, 529, 574, 669
HpyCH4III ACNGT 3 cut(s) 163, 990, 1014
HpyCH4IV ACGT 4 cut(s) 108, 381, 424, 1017
HpyCH4V TGCA 3 cut(s) 246, 634, 821
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 243
HpyF3I CTNAG 4 cut(s) 509, 609, 842, 891
HpySE526I ACGT 4 cut(s) 108, 381, 424, 1017
Hsp92II CATG 5 cut(s) 451, 457, 559, 666, 868
HspAI GCGC 1 cut(s) 235
Kzo9I GATC 7 cut(s) 69, 142, 200, 395, 717, 837, 903
LmnI GCTCC 3 cut(s) 501, 868, 1083
LweI GCATC 3 cut(s) 14, 621, 793
MaeI CTAG 3 cut(s) 45, 191, 551
MaeII ACGT 4 cut(s) 108, 381, 424, 1017
MaeIII GTNAC 5 cut(s) 104, 193, 377, 425, 955
MalI GATC 7 cut(s) 71, 144, 202, 397, 719, 839, 905
MboI GATC 7 cut(s) 69, 142, 200, 395, 717, 837, 903
MboII GAAGA 8 cut(s) 134, 335, 359, 478, 601, 635, 812, 913
MflI RGATCY 3 cut(s) 395, 837, 903
MhlI GDGCHC 3 cut(s) 44, 498, 873
MluCI AATT 5 cut(s) 326, 649, 932, 945, 1090
MlyI GAGTC 2 cut(s) 89, 533
MroXI GAANNNNTTC 1 cut(s) 518
MseI TTAA 5 cut(s) 86, 333, 738, 762, 816
MslI CAYNNNNRTG 2 cut(s) 179, 452
MspI CCGG 1 cut(s) 714
MspR9I CCNGG 4 cut(s) 27, 65, 375, 715
MvaI CCWGG 3 cut(s) 27, 65, 375
MwoI GCNNNNNNNGC 2 cut(s) 39, 243
NciI CCSGG 1 cut(s) 715
NcoI CCATGG 1 cut(s) 555
NdeII GATC 7 cut(s) 69, 142, 200, 395, 717, 837, 903
NlaIII CATG 5 cut(s) 451, 457, 559, 666, 868
NlaIV GGNNCC 3 cut(s) 397, 783, 1079
NmuCI GTSAC 3 cut(s) 377, 425, 955
NspV TTCGAA 1 cut(s) 120
PceI AGGCCT 1 cut(s) 373
PdmI GAANNNNTTC 1 cut(s) 518
PfeI GAWTC 3 cut(s) 215, 258, 441
PfoI TCCNGGA 2 cut(s) 63, 713
PleI GAGTC 2 cut(s) 88, 532
PpsI GAGTC 2 cut(s) 88, 532
Psp124BI GAGCTC 2 cut(s) 44, 498
Psp6I CCWGG 3 cut(s) 25, 63, 373
PspFI CCCAGC 1 cut(s) 12
PspGI CCWGG 3 cut(s) 25, 63, 373
PspN4I GGNNCC 3 cut(s) 397, 783, 1079
PspPI GGNCC 2 cut(s) 950, 1066
PstNI CAGNNNCTG 1 cut(s) 218
PsuI RGATCY 3 cut(s) 395, 837, 903
RsaI GTAC 3 cut(s) 314, 857, 1016
RsaNI GTAC 3 cut(s) 313, 856, 1015
RseI CAYNNNNRTG 2 cut(s) 179, 452
SacI GAGCTC 2 cut(s) 44, 498
SaqAI TTAA 5 cut(s) 86, 333, 738, 762, 816
Sau3AI GATC 7 cut(s) 69, 142, 200, 395, 717, 837, 903
Sau96I GGNCC 2 cut(s) 950, 1066
SchI GAGTC 2 cut(s) 89, 533
ScrFI CCNGG 4 cut(s) 27, 65, 375, 715
SduI GDGCHC 3 cut(s) 44, 498, 873
SfaNI GCATC 3 cut(s) 14, 621, 793
SfcI CTRYAG 1 cut(s) 755
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 2 cut(s) 950, 1066
SmiMI CAYNNNNRTG 2 cut(s) 179, 452
SpeI ACTAGT 1 cut(s) 190
Sse9I AATT 5 cut(s) 326, 649, 932, 945, 1090
SseBI AGGCCT 1 cut(s) 373
SsiI CCGC 1 cut(s) 1036
SspMI CTAG 3 cut(s) 45, 191, 551
SstI GAGCTC 2 cut(s) 44, 498
StuI AGGCCT 1 cut(s) 373
StyD4I CCNGG 4 cut(s) 25, 63, 373, 713
StyI CCWWGG 2 cut(s) 342, 555
TaaI ACNGT 3 cut(s) 163, 990, 1014
TaiI ACGT 4 cut(s) 111, 384, 427, 1020
TaqI TCGA 4 cut(s) 120, 177, 522, 1047
TasI AATT 5 cut(s) 326, 649, 932, 945, 1090
TatI WGTACW 1 cut(s) 855
TfiI GAWTC 3 cut(s) 215, 258, 441
Tru1I TTAA 5 cut(s) 86, 333, 738, 762, 816
Tru9I TTAA 5 cut(s) 86, 333, 738, 762, 816
TscAI CASTG 3 cut(s) 645, 960, 993
TseFI GTSAC 3 cut(s) 377, 425, 955
Tsp45I GTSAC 3 cut(s) 377, 425, 955
TspDTI ATGAA 7 cut(s) 126, 371, 455, 470, 492, 651, 662
TspGWI ACGGA 3 cut(s) 101, 294, 785
TspRI CASTG 3 cut(s) 645, 960, 993
VpaK11BI GGWCC 2 cut(s) 950, 1066
XapI RAATTY 2 cut(s) 326, 932
XmiI GTMKAC 1 cut(s) 754
XmnI GAANNNNTTC 1 cut(s) 518
XspI CTAG 3 cut(s) 45, 191, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.