RchiOBHm_Chr2g0132141

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001297
Physical Location & Seq
Reverse (-)
48527564 .. 48529429
1866 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50342

Sequence Viewer

Length: 906 bp
ATGGTATCATCAAAACCAGCCAGCTGCTTTGATCCCTCTCATCATCACTCAGGAGTCAGTATTATTGAGGAACTCATCACTGATGATTCAGTGATCGAAGTCCCGCAAAACTATGTTATCCCAGACCAGGAGTCTTCAATCTCTCATGGGACTATCCTTGCCACAATTCCCACCATTGACATGAGACAGTTGGTCCTAATCAATCAATTTGCAAACAACGATGAACTGGAGAAGTTGCACTCAACATGCAAAGAATGGGGAATTTTTCAGTTGGTTAACCATGGAGTTAGCTCTGCACTACTGGACAAAGTGAAGCAAGAATTAGAGGATTTTTTCAAGCTTCCCTTGGAAGAGAAAATGAGATACAAAGTAAGGCCAGGTGATGTTGAAGGCTATGGAACTGTTGTCAAATCGCAAGATCAAAAACTCGACTGGGGTAACAGGTTCTTCATGTTTACCAACCCGAGAAGGAAACCCCATCTTTTCCCGGAGCTGCCTACATCCCTGAGGAATACCTTGGAGACATACATGTTGGAATTACAAAAGATTGCCCGAACCCTTTTAGGGTTCATGGGAAAACTTCTAAAGATGAAGATGGAGGAGATGGAAGAGTTGTTTGAAGATGGTCTGCAGTCACTGAGGATGACTCACTATCCTCCATGCCCAAAACCAGAGCTAGTTGTTGGCCTCAGGCCACATTCTGATGGTAGTGGCATCACTATTCTCCACCAGGTTGATGGAGTTCAAGGCCTTCAAATTAAAAAAGATGGGGCTTGGTCCCCTGTGATATTTGACCATGGTGCCTTTGTTGTCAATGTTGGAGACATTCTAGAGGTTTTTGATGCTGTTCTCTTCACCCTCCATTTTCCTCTCCTCAAAAAGACATGTTGTCTATTGGTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

301

Amino Acids

34.11

Weight (kDa)

5.64

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 56 - 159 2.3e-27 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 211 - 280 9e-18 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 800
AciI CCGC 1 cut(s) 104
AclWI GGATC 1 cut(s) 26
AcsI RAATTY 1 cut(s) 261
AfiI CCNNNNNNNGG 2 cut(s) 127, 564
AflIII ACRYGT 2 cut(s) 528, 884
AgsI TTSAA 6 cut(s) 138, 337, 389, 620, 746, 755
AhdI GACNNNNNGTC 3 cut(s) 130, 191, 888
AjnI CCWGG 3 cut(s) 126, 376, 729
AluBI AGCT 5 cut(s) 24, 291, 340, 493, 676
AluI AGCT 5 cut(s) 24, 291, 340, 493, 676
Alw26I GTCTC 3 cut(s) 178, 515, 816
AlwI GGATC 1 cut(s) 26
AlwNI CAGNNNCTG 1 cut(s) 637
Ama87I CYCGRG 1 cut(s) 463
AoxI GGCC 4 cut(s) 374, 685, 692, 748
ApeKI GCWGC 2 cut(s) 24, 493
ApoI RAATTY 1 cut(s) 261
AspS9I GGNCC 2 cut(s) 193, 777
AsuC2I CCSGG 1 cut(s) 488
AsuHPI GGTGA 2 cut(s) 392, 847
AvaI CYCGRG 1 cut(s) 463
AvaII GGWCC 2 cut(s) 193, 777
AxyI CCTNAGG 2 cut(s) 506, 689
BanI GGYRCC 1 cut(s) 800
BbsI GAAGAC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 11, 480
BccI CCATC 7 cut(s) 486, 589, 598, 617, 698, 731, 761
BciT130I CCWGG 3 cut(s) 128, 378, 731
BcnI CCSGG 1 cut(s) 488
BcoDI GTCTC 3 cut(s) 178, 515, 816
BfaI CTAG 2 cut(s) 677, 830
BfmI CTRYAG 1 cut(s) 629
BisI GCNGC 2 cut(s) 25, 494
BlsI GCNGC 2 cut(s) 26, 495
Bme1390I CCNGG 4 cut(s) 128, 378, 488, 731
Bme18I GGWCC 2 cut(s) 193, 777
BmeRI GACNNNNNGTC 3 cut(s) 130, 191, 888
BmeT110I CYCGRG 1 cut(s) 463
BmgT120I GGNCC 2 cut(s) 193, 777
BmiI GGNNCC 2 cut(s) 779, 802
BmrFI CCNGG 4 cut(s) 128, 378, 488, 731
BmrI ACTGGG 1 cut(s) 442
BmsI GCATC 2 cut(s) 723, 832
BmuI ACTGGG 1 cut(s) 442
BpiI GAAGAC 1 cut(s) 126
BplI GAGNNNNNCTC 2 cut(s) 631, 663
BpmI CTGGAG 1 cut(s) 248
BpuMI CCSGG 1 cut(s) 488
BsaJI CCNNGG 4 cut(s) 280, 345, 516, 796
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 564
Bse1I ACTGG 3 cut(s) 231, 306, 437
Bse21I CCTNAGG 2 cut(s) 506, 689
BseBI CCWGG 3 cut(s) 128, 378, 731
BseDI CCNNGG 4 cut(s) 280, 345, 516, 796
BseGI GGATG 2 cut(s) 500, 648
BseLI CCNNNNNNNGG 2 cut(s) 127, 564
BseMII CTCAG 4 cut(s) 63, 497, 629, 703
BseNI ACTGG 3 cut(s) 231, 306, 437
BseRI GAGGAG 2 cut(s) 614, 863
BseXI GCAGC 2 cut(s) 11, 480
BsgI GTGCAG 1 cut(s) 279
BshFI GGCC 4 cut(s) 376, 687, 694, 750
BshNI GGYRCC 1 cut(s) 800
BsiHKCI CYCGRG 1 cut(s) 463
BsiSI CCGG 1 cut(s) 488
BslFI GGGAC 3 cut(s) 86, 163, 763
BslI CCNNNNNNNGG 2 cut(s) 127, 564
BsmAI GTCTC 3 cut(s) 178, 515, 816
BsmFI GGGAC 3 cut(s) 86, 163, 763
BsnI GGCC 4 cut(s) 376, 687, 694, 750
BsoBI CYCGRG 1 cut(s) 463
Bsp143I GATC 3 cut(s) 31, 93, 418
Bsp19I CCATGG 2 cut(s) 280, 796
BspACI CCGC 1 cut(s) 104
BspANI GGCC 4 cut(s) 376, 687, 694, 750
BspCNI CTCAG 4 cut(s) 62, 498, 630, 702
BspLI GGNNCC 2 cut(s) 779, 802
BspMAI CTGCAG 1 cut(s) 633
BspPI GGATC 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 800
BsrI ACTGG 3 cut(s) 231, 306, 437
BssECI CCNNGG 4 cut(s) 280, 345, 516, 796
BssMI GATC 3 cut(s) 31, 93, 418
BssT1I CCWWGG 4 cut(s) 280, 345, 516, 796
Bst2UI CCWGG 3 cut(s) 128, 378, 731
Bst4CI ACNGT 2 cut(s) 189, 403
Bst6I CTCTTC 3 cut(s) 345, 603, 857
BstC8I GCNNGC 1 cut(s) 22
BstDEI CTNAG 4 cut(s) 49, 506, 638, 689
BstDSI CCRYGG 2 cut(s) 280, 796
BstF5I GGATG 2 cut(s) 500, 648
BstKTI GATC 3 cut(s) 34, 96, 421
BstMAI GTCTC 3 cut(s) 178, 515, 816
BstMBI GATC 3 cut(s) 31, 93, 418
BstNI CCWGG 3 cut(s) 128, 378, 731
BstNSI RCATGY 3 cut(s) 249, 532, 888
BstSCI CCNGG 4 cut(s) 126, 376, 486, 729
BstSFI CTRYAG 1 cut(s) 629
BstV1I GCAGC 2 cut(s) 11, 480
BstV2I GAAGAC 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 737
Bsu36I CCTNAGG 2 cut(s) 506, 689
BsuRI GGCC 4 cut(s) 376, 687, 694, 750
BtgI CCRYGG 2 cut(s) 280, 796
BtsCI GGATG 2 cut(s) 500, 648
BtsIMutI CAGTG 3 cut(s) 78, 96, 635
Cac8I GCNNGC 1 cut(s) 22
CaiI CAGNNNCTG 1 cut(s) 637
Cfr13I GGNCC 2 cut(s) 193, 777
CsiI ACCWGGT 1 cut(s) 729
DdeI CTNAG 4 cut(s) 49, 506, 638, 689
DpnI GATC 3 cut(s) 33, 95, 420
DpnII GATC 3 cut(s) 31, 93, 418
DriI GACNNNNNGTC 3 cut(s) 130, 191, 888
Eam1104I CTCTTC 3 cut(s) 345, 603, 857
Eam1105I GACNNNNNGTC 3 cut(s) 130, 191, 888
EarI CTCTTC 3 cut(s) 345, 603, 857
Eco130I CCWWGG 4 cut(s) 280, 345, 516, 796
Eco147I AGGCCT 1 cut(s) 750
Eco47I GGWCC 2 cut(s) 193, 777
Eco81I CCTNAGG 2 cut(s) 506, 689
Eco88I CYCGRG 1 cut(s) 463
EcoRII CCWGG 3 cut(s) 126, 376, 729
EcoT14I CCWWGG 4 cut(s) 280, 345, 516, 796
ErhI CCWWGG 4 cut(s) 280, 345, 516, 796
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 3 cut(s) 86, 163, 763
FauI CCCGC 1 cut(s) 111
Fnu4HI GCNGC 2 cut(s) 25, 494
FokI GGATG 2 cut(s) 487, 655
Fsp4HI GCNGC 2 cut(s) 25, 494
FspBI CTAG 2 cut(s) 677, 830
GluI GCNGC 2 cut(s) 25, 494
GsuI CTGGAG 1 cut(s) 248
HaeIII GGCC 4 cut(s) 376, 687, 694, 750
HapII CCGG 1 cut(s) 488
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 4 cut(s) 54, 86, 131, 646
HpaI GTTAAC 1 cut(s) 277
HpaII CCGG 1 cut(s) 488
HphI GGTGA 2 cut(s) 392, 847
Hpy166II GTNNAC 2 cut(s) 277, 456
Hpy188I TCNGA 1 cut(s) 703
Hpy188III TCNNGA 2 cut(s) 51, 830
Hpy8I GTNNAC 2 cut(s) 277, 456
HpyAV CCTTC 3 cut(s) 383, 462, 761
HpyCH4III ACNGT 2 cut(s) 189, 403
HpyCH4V TGCA 5 cut(s) 212, 238, 249, 296, 631
HpyF3I CTNAG 4 cut(s) 49, 506, 638, 689
KspAI GTTAAC 1 cut(s) 277
Kzo9I GATC 3 cut(s) 31, 93, 418
LmnI GCTCC 1 cut(s) 490
Lsp1109I GCAGC 2 cut(s) 11, 480
LweI GCATC 2 cut(s) 723, 832
MabI ACCWGGT 1 cut(s) 729
MaeI CTAG 2 cut(s) 677, 830
MaeIII GTNAC 2 cut(s) 437, 633
MalI GATC 3 cut(s) 33, 95, 420
MboI GATC 3 cut(s) 31, 93, 418
MboII GAAGA 7 cut(s) 126, 362, 439, 604, 620, 632, 844
MluCI AATT 6 cut(s) 165, 206, 261, 320, 536, 756
MlyI GAGTC 3 cut(s) 63, 140, 640
MmeI TCCRAC 2 cut(s) 513, 799
MseI TTAA 2 cut(s) 276, 759
MslI CAYNNNNRTG 2 cut(s) 179, 702
MspA1I CMGCKG 1 cut(s) 24
MspI CCGG 1 cut(s) 488
MspR9I CCNGG 4 cut(s) 128, 378, 488, 731
MvaI CCWGG 3 cut(s) 128, 378, 731
NciI CCSGG 1 cut(s) 488
NcoI CCATGG 2 cut(s) 280, 796
NdeII GATC 3 cut(s) 31, 93, 418
NlaIV GGNNCC 2 cut(s) 779, 802
NmuCI GTSAC 1 cut(s) 633
NspI RCATGY 3 cut(s) 249, 532, 888
PceI AGGCCT 1 cut(s) 750
PciI ACATGT 2 cut(s) 528, 884
PfeI GAWTC 1 cut(s) 86
PfoI TCCNGGA 1 cut(s) 486
PkrI GCNGC 2 cut(s) 26, 495
PleI GAGTC 3 cut(s) 62, 139, 640
PpsI GAGTC 3 cut(s) 62, 139, 640
PscI ACATGT 2 cut(s) 528, 884
Psp6I CCWGG 3 cut(s) 126, 376, 729
PspGI CCWGG 3 cut(s) 126, 376, 729
PspN4I GGNNCC 2 cut(s) 779, 802
PspPI GGNCC 2 cut(s) 193, 777
PstI CTGCAG 1 cut(s) 633
PstNI CAGNNNCTG 1 cut(s) 637
PvuII CAGCTG 1 cut(s) 24
RseI CAYNNNNRTG 2 cut(s) 179, 702
SaqAI TTAA 2 cut(s) 276, 759
SatI GCNGC 2 cut(s) 25, 494
Sau3AI GATC 3 cut(s) 31, 93, 418
Sau96I GGNCC 2 cut(s) 193, 777
SchI GAGTC 3 cut(s) 63, 140, 640
ScrFI CCNGG 4 cut(s) 128, 378, 488, 731
SexAI ACCWGGT 1 cut(s) 729
SfaNI GCATC 2 cut(s) 723, 832
SfcI CTRYAG 1 cut(s) 629
SinI GGWCC 2 cut(s) 193, 777
SmiMI CAYNNNNRTG 2 cut(s) 179, 702
Sse9I AATT 6 cut(s) 165, 206, 261, 320, 536, 756
SseBI AGGCCT 1 cut(s) 750
SsiI CCGC 1 cut(s) 104
SspMI CTAG 2 cut(s) 677, 830
StuI AGGCCT 1 cut(s) 750
StyD4I CCNGG 4 cut(s) 126, 376, 486, 729
StyI CCWWGG 4 cut(s) 280, 345, 516, 796
TaaI ACNGT 2 cut(s) 189, 403
TaqI TCGA 2 cut(s) 96, 429
TasI AATT 6 cut(s) 165, 206, 261, 320, 536, 756
TfiI GAWTC 1 cut(s) 86
Tru1I TTAA 2 cut(s) 276, 759
Tru9I TTAA 2 cut(s) 276, 759
TscAI CASTG 3 cut(s) 85, 96, 642
TseFI GTSAC 1 cut(s) 633
TseI GCWGC 2 cut(s) 24, 493
Tsp45I GTSAC 1 cut(s) 633
TspDTI ATGAA 4 cut(s) 237, 439, 559, 605
TspRI CASTG 3 cut(s) 85, 96, 642
VpaK11BI GGWCC 2 cut(s) 193, 777
XapI RAATTY 1 cut(s) 261
XbaI TCTAGA 1 cut(s) 829
XceI RCATGY 3 cut(s) 249, 532, 888
XcmI CCANNNNNNNNNTGG 1 cut(s) 734
XspI CTAG 2 cut(s) 677, 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.