Rw2G028910

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001297
Physical Location & Seq
Reverse (-)
46410430 .. 46414233
3804 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G028910.1

Sequence Viewer

Length: 1074 bp
ATGGTATCATCAAAACCAGCCTGCTTCGGCCCCTCTCATCATCACTCAGGAGTCAGTTGTATTGAGGAATTCGTCACCGATGATTCAGTGATCGAAGTCCCGCAAAATTATGTTATCTCAGATCAGGAGTCTCCAATCTCTGATGGGACTATCCTTGCCACAATCCCCACCATTGACATGAGACAGTTGGTCCTAATCAATCAGTCTGCAAACAACGATGAACTTGAGAAGTTGCACTCAACATGCAAAGAATGGGGAATTTTTCAGTTGGTTAACCATGGAGTTAGCTCTGCACTACTGGACAAAGTGAAGCAAGAATTAGAGGATTTTTTCAAGCTTCCCTTGGAAGAGAAAATGAGATACAATGTAAGGCCAGGTGATGTTGAAGGCTATGGAACTGTGGTCAAATCCCAAGATCAAAAACTCGACTGGGGTAACAGGTTCTTCATGTTTACCAACCCGAGAAGGAAACCCCATCTTTTCCCGGAGCTCCCTACATCCCTGAGGAATACCTTAGAGACATACATGTTGGAATTACAAAGGATTGCCCGGACCCTTTTAGGGTTGATGGGAAAACTTCTAAAGATGAAGATGGAGGAGATGGAAAAGTTGTTTGAAGATGGTCTGCAGTCACTGAGGATGACTCACTATCCTCCATGCCCAAAACCAGAACTAGTTATTGGCCTCAGGCCTCATTCTGATGGTAGTGGCATCACTATTCTCCACCAGGTTGGTGGAGTTCAAGGCCTTCAAATTAAAAAAGATGGGGCTTGGTTCCCGGTGATGTTTGACCATGGTGCCTTTGTTGTCAATGTTGGAGACATTCTAGAGATTCTGAGTGATGGGCTTTATAAAAGTATCGAGCACAAAGTGACTGTGAACCCAGAGAATGAAAGGATTTCAATTGCTATGTTCTTCATGCCCAAACTTGAGGCAGAAATTGGACCTGTGACCGCCATGAGAGATCCTCAGAAGCCACCATCTTATGGGAAAATAGGAATGGAAAAATTTGTGGAGAAGTACTTTGCTCATAACCTTAATGGAAAAACACCCTTGGAGCGAATGAAAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

357

Amino Acids

40.43

Weight (kDa)

5.97

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 55 - 158 5.1e-28 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 210 - 308 8.3e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 852
AccB1I GGYRCC 1 cut(s) 797
AccB7I CCANNNNNTGG 1 cut(s) 986
AciI CCGC 2 cut(s) 101, 954
AclWI GGATC 1 cut(s) 959
AcsI RAATTY 3 cut(s) 68, 258, 1007
AdeI CACNNNGTG 1 cut(s) 871
AfaI GTAC 1 cut(s) 1022
AfiI CCNNNNNNNGG 2 cut(s) 561, 986
AflIII ACRYGT 1 cut(s) 525
AgsI TTSAA 6 cut(s) 334, 386, 617, 743, 752, 903
AhdI GACNNNNNGTC 1 cut(s) 188
AhlI ACTAGT 1 cut(s) 673
AjnI CCWGG 2 cut(s) 373, 726
AjuI GAANNNNNNNTTGG 2 cut(s) 663, 695
AluBI AGCT 3 cut(s) 288, 337, 490
AluI AGCT 3 cut(s) 288, 337, 490
Alw21I GWGCWC 2 cut(s) 492, 867
Alw26I GTCTC 4 cut(s) 135, 175, 512, 813
AlwI GGATC 1 cut(s) 959
AlwNI CAGNNNCTG 1 cut(s) 634
Ama87I CYCGRG 1 cut(s) 460
AoxI GGCC 5 cut(s) 28, 371, 682, 689, 745
ApoI RAATTY 3 cut(s) 68, 258, 1007
AspS9I GGNCC 4 cut(s) 29, 190, 552, 944
AsuC2I CCSGG 3 cut(s) 485, 550, 779
AsuHPI GGTGA 3 cut(s) 67, 389, 793
AvaI CYCGRG 1 cut(s) 460
AvaII GGWCC 3 cut(s) 190, 552, 944
AxyI CCTNAGG 2 cut(s) 503, 686
BanI GGYRCC 1 cut(s) 797
BanII GRGCYC 1 cut(s) 492
Bbv12I GWGCWC 2 cut(s) 492, 867
BciT130I CCWGG 2 cut(s) 375, 728
BcnI CCSGG 3 cut(s) 485, 550, 779
BcoDI GTCTC 4 cut(s) 135, 175, 512, 813
BcuI ACTAGT 1 cut(s) 673
BfaI CTAG 2 cut(s) 674, 827
BfmI CTRYAG 1 cut(s) 626
BmcAI AGTACT 1 cut(s) 1022
Bme1390I CCNGG 5 cut(s) 375, 485, 550, 728, 779
Bme18I GGWCC 3 cut(s) 190, 552, 944
BmeRI GACNNNNNGTC 1 cut(s) 188
BmeT110I CYCGRG 1 cut(s) 460
BmgT120I GGNCC 4 cut(s) 29, 190, 552, 944
BmiI GGNNCC 4 cut(s) 31, 554, 776, 799
BmrFI CCNGG 5 cut(s) 375, 485, 550, 728, 779
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 1 cut(s) 720
BmuI ACTGGG 1 cut(s) 439
BplI GAGNNNNNCTC 4 cut(s) 628, 660, 952, 984
BpuEI CTTGAG 2 cut(s) 245, 950
BpuMI CCSGG 3 cut(s) 485, 550, 779
BsaJI CCNNGG 4 cut(s) 277, 342, 793, 1053
Bsc4I CCNNNNNNNGG 2 cut(s) 561, 986
Bse1I ACTGG 2 cut(s) 303, 434
Bse21I CCTNAGG 2 cut(s) 503, 686
BseBI CCWGG 2 cut(s) 375, 728
BseDI CCNNGG 4 cut(s) 277, 342, 793, 1053
BseGI GGATG 2 cut(s) 497, 645
BseLI CCNNNNNNNGG 2 cut(s) 561, 986
BseMII CTCAG 7 cut(s) 60, 132, 494, 626, 700, 827, 983
BseNI ACTGG 2 cut(s) 303, 434
BseRI GAGGAG 1 cut(s) 611
BsgI GTGCAG 1 cut(s) 276
BshFI GGCC 5 cut(s) 30, 373, 684, 691, 747
BshNI GGYRCC 1 cut(s) 797
BsiHKAI GWGCWC 2 cut(s) 492, 867
BsiHKCI CYCGRG 1 cut(s) 460
BsiSI CCGG 3 cut(s) 485, 550, 779
BslFI GGGAC 2 cut(s) 83, 160
BslI CCNNNNNNNGG 2 cut(s) 561, 986
BsmAI GTCTC 4 cut(s) 135, 175, 512, 813
BsmFI GGGAC 2 cut(s) 83, 160
BsnI GGCC 5 cut(s) 30, 373, 684, 691, 747
BsoBI CYCGRG 1 cut(s) 460
Bsp1286I GDGCHC 2 cut(s) 492, 867
Bsp143I GATC 4 cut(s) 90, 121, 415, 964
Bsp19I CCATGG 2 cut(s) 277, 793
BspACI CCGC 2 cut(s) 101, 954
BspANI GGCC 5 cut(s) 30, 373, 684, 691, 747
BspCNI CTCAG 7 cut(s) 59, 131, 495, 627, 699, 828, 982
BspLI GGNNCC 4 cut(s) 31, 554, 776, 799
BspMAI CTGCAG 1 cut(s) 630
BspPI GGATC 1 cut(s) 959
BspT107I GGYRCC 1 cut(s) 797
BsrI ACTGG 2 cut(s) 303, 434
BssECI CCNNGG 4 cut(s) 277, 342, 793, 1053
BssMI GATC 4 cut(s) 90, 121, 415, 964
BssT1I CCWWGG 4 cut(s) 277, 342, 793, 1053
Bst2UI CCWGG 2 cut(s) 375, 728
Bst4CI ACNGT 3 cut(s) 186, 400, 877
Bst6I CTCTTC 1 cut(s) 342
BstC8I GCNNGC 1 cut(s) 22
BstDEI CTNAG 8 cut(s) 46, 118, 503, 514, 635, 686, 836, 969
BstDSI CCRYGG 2 cut(s) 277, 793
BstF5I GGATG 2 cut(s) 497, 645
BstKTI GATC 4 cut(s) 93, 124, 418, 967
BstMAI GTCTC 4 cut(s) 135, 175, 512, 813
BstMBI GATC 4 cut(s) 90, 121, 415, 964
BstNI CCWGG 2 cut(s) 375, 728
BstNSI RCATGY 2 cut(s) 246, 529
BstSCI CCNGG 5 cut(s) 373, 483, 548, 726, 777
BstSFI CTRYAG 1 cut(s) 626
BstX2I RGATCY 1 cut(s) 964
BstXI CCANNNNNNTGG 2 cut(s) 731, 734
BstYI RGATCY 1 cut(s) 964
Bsu36I CCTNAGG 2 cut(s) 503, 686
BsuRI GGCC 5 cut(s) 30, 373, 684, 691, 747
BtgI CCRYGG 2 cut(s) 277, 793
BtsCI GGATG 2 cut(s) 497, 645
BtsIMutI CAGTG 2 cut(s) 93, 632
Cac8I GCNNGC 1 cut(s) 22
CaiI CAGNNNCTG 1 cut(s) 634
Cfr13I GGNCC 4 cut(s) 29, 190, 552, 944
CsiI ACCWGGT 1 cut(s) 726
Csp6I GTAC 1 cut(s) 1021
CviAII CATG 9 cut(s) 178, 243, 278, 448, 526, 657, 794, 919, 958
CviQI GTAC 1 cut(s) 1021
DdeI CTNAG 8 cut(s) 46, 118, 503, 514, 635, 686, 836, 969
DpnI GATC 4 cut(s) 92, 123, 417, 966
DpnII GATC 4 cut(s) 90, 121, 415, 964
DraIII CACNNNGTG 1 cut(s) 871
DriI GACNNNNNGTC 1 cut(s) 188
Eam1104I CTCTTC 1 cut(s) 342
Eam1105I GACNNNNNGTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 342
Ecl136II GAGCTC 1 cut(s) 490
Eco130I CCWWGG 4 cut(s) 277, 342, 793, 1053
Eco147I AGGCCT 2 cut(s) 691, 747
Eco24I GRGCYC 1 cut(s) 492
Eco47I GGWCC 3 cut(s) 190, 552, 944
Eco53kI GAGCTC 1 cut(s) 490
Eco81I CCTNAGG 2 cut(s) 503, 686
Eco88I CYCGRG 1 cut(s) 460
EcoICRI GAGCTC 1 cut(s) 490
EcoRI GAATTC 1 cut(s) 68
EcoRII CCWGG 2 cut(s) 373, 726
EcoT14I CCWWGG 4 cut(s) 277, 342, 793, 1053
EcoT38I GRGCYC 1 cut(s) 492
ErhI CCWWGG 4 cut(s) 277, 342, 793, 1053
FaeI CATG 9 cut(s) 181, 246, 281, 451, 529, 660, 797, 922, 961
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FaqI GGGAC 2 cut(s) 83, 160
FatI CATG 9 cut(s) 177, 242, 277, 447, 525, 656, 793, 918, 957
FauI CCCGC 1 cut(s) 108
FokI GGATG 2 cut(s) 484, 652
FriOI GRGCYC 1 cut(s) 492
FspBI CTAG 2 cut(s) 674, 827
HaeIII GGCC 5 cut(s) 30, 373, 684, 691, 747
HapII CCGG 3 cut(s) 485, 550, 779
Hin1II CATG 9 cut(s) 181, 246, 281, 451, 529, 660, 797, 922, 961
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 5 cut(s) 51, 83, 128, 643, 832
HpaI GTTAAC 1 cut(s) 274
HpaII CCGG 3 cut(s) 485, 550, 779
HphI GGTGA 3 cut(s) 67, 389, 793
Hpy166II GTNNAC 3 cut(s) 274, 453, 880
Hpy188I TCNGA 5 cut(s) 121, 142, 700, 837, 972
Hpy188III TCNNGA 3 cut(s) 48, 125, 827
Hpy8I GTNNAC 3 cut(s) 274, 453, 880
HpyAV CCTTC 3 cut(s) 380, 459, 758
HpyCH4III ACNGT 3 cut(s) 186, 400, 877
HpyCH4V TGCA 5 cut(s) 209, 235, 246, 293, 628
HpyF3I CTNAG 8 cut(s) 46, 118, 503, 514, 635, 686, 836, 969
Hsp92II CATG 9 cut(s) 181, 246, 281, 451, 529, 660, 797, 922, 961
KspAI GTTAAC 1 cut(s) 274
Kzo9I GATC 4 cut(s) 90, 121, 415, 964
LmnI GCTCC 3 cut(s) 487, 495, 1057
LweI GCATC 1 cut(s) 720
MabI ACCWGGT 1 cut(s) 726
MaeI CTAG 2 cut(s) 674, 827
MaeIII GTNAC 5 cut(s) 73, 434, 630, 871, 949
MalI GATC 4 cut(s) 92, 123, 417, 966
MboI GATC 4 cut(s) 90, 121, 415, 964
MboII GAAGA 5 cut(s) 359, 436, 601, 629, 907
MfeI CAATTG 1 cut(s) 903
MflI RGATCY 1 cut(s) 964
MhlI GDGCHC 2 cut(s) 492, 867
MluCI AATT 9 cut(s) 68, 106, 258, 317, 533, 753, 903, 939, 1007
MlyI GAGTC 3 cut(s) 60, 137, 637
MmeI TCCRAC 2 cut(s) 510, 796
MseI TTAA 3 cut(s) 273, 756, 1038
MslI CAYNNNNRTG 2 cut(s) 176, 699
MspI CCGG 3 cut(s) 485, 550, 779
MspR9I CCNGG 5 cut(s) 375, 485, 550, 728, 779
MunI CAATTG 1 cut(s) 903
MvaI CCWGG 2 cut(s) 375, 728
NciI CCSGG 3 cut(s) 485, 550, 779
NcoI CCATGG 2 cut(s) 277, 793
NdeII GATC 4 cut(s) 90, 121, 415, 964
NlaIII CATG 9 cut(s) 181, 246, 281, 451, 529, 660, 797, 922, 961
NlaIV GGNNCC 4 cut(s) 31, 554, 776, 799
NmuCI GTSAC 4 cut(s) 73, 630, 871, 949
NspI RCATGY 2 cut(s) 246, 529
PceI AGGCCT 2 cut(s) 691, 747
PciI ACATGT 1 cut(s) 525
PfeI GAWTC 2 cut(s) 83, 832
PflMI CCANNNNNTGG 1 cut(s) 986
PfoI TCCNGGA 1 cut(s) 483
PleI GAGTC 3 cut(s) 59, 136, 637
PpsI GAGTC 3 cut(s) 59, 136, 637
PscI ACATGT 1 cut(s) 525
PsiI TTATAA 1 cut(s) 852
Psp124BI GAGCTC 1 cut(s) 492
Psp6I CCWGG 2 cut(s) 373, 726
PspGI CCWGG 2 cut(s) 373, 726
PspN4I GGNNCC 4 cut(s) 31, 554, 776, 799
PspPI GGNCC 4 cut(s) 29, 190, 552, 944
PstI CTGCAG 1 cut(s) 630
PstNI CAGNNNCTG 1 cut(s) 634
PsuI RGATCY 1 cut(s) 964
RsaI GTAC 1 cut(s) 1022
RsaNI GTAC 1 cut(s) 1021
RseI CAYNNNNRTG 2 cut(s) 176, 699
SacI GAGCTC 1 cut(s) 492
SaqAI TTAA 3 cut(s) 273, 756, 1038
Sau3AI GATC 4 cut(s) 90, 121, 415, 964
Sau96I GGNCC 4 cut(s) 29, 190, 552, 944
ScaI AGTACT 1 cut(s) 1022
SchI GAGTC 3 cut(s) 60, 137, 637
ScrFI CCNGG 5 cut(s) 375, 485, 550, 728, 779
SduI GDGCHC 2 cut(s) 492, 867
SetI ASST 9 cut(s) 290, 339, 379, 443, 492, 515, 732, 949, 1038
SexAI ACCWGGT 1 cut(s) 726
SfaNI GCATC 1 cut(s) 720
SfcI CTRYAG 1 cut(s) 626
SinI GGWCC 3 cut(s) 190, 552, 944
SmiMI CAYNNNNRTG 2 cut(s) 176, 699
SmlI CTYRAG 2 cut(s) 224, 929
SmoI CTYRAG 2 cut(s) 224, 929
SpeI ACTAGT 1 cut(s) 673
Sse9I AATT 9 cut(s) 68, 106, 258, 317, 533, 753, 903, 939, 1007
SseBI AGGCCT 2 cut(s) 691, 747
SsiI CCGC 2 cut(s) 101, 954
SspMI CTAG 2 cut(s) 674, 827
SstI GAGCTC 1 cut(s) 492
StuI AGGCCT 2 cut(s) 691, 747
StyD4I CCNGG 5 cut(s) 373, 483, 548, 726, 777
StyI CCWWGG 4 cut(s) 277, 342, 793, 1053
TaaI ACNGT 3 cut(s) 186, 400, 877
TaqI TCGA 3 cut(s) 93, 426, 861
TasI AATT 9 cut(s) 68, 106, 258, 317, 533, 753, 903, 939, 1007
TatI WGTACW 1 cut(s) 1020
TfiI GAWTC 2 cut(s) 83, 832
Tru1I TTAA 3 cut(s) 273, 756, 1038
Tru9I TTAA 3 cut(s) 273, 756, 1038
TscAI CASTG 2 cut(s) 93, 639
TseFI GTSAC 4 cut(s) 73, 630, 871, 949
Tsp45I GTSAC 4 cut(s) 73, 630, 871, 949
TspDTI ATGAA 5 cut(s) 234, 436, 602, 906, 907
TspRI CASTG 2 cut(s) 93, 639
Van91I CCANNNNNTGG 1 cut(s) 986
VpaK11BI GGWCC 3 cut(s) 190, 552, 944
XapI RAATTY 3 cut(s) 68, 258, 1007
XbaI TCTAGA 1 cut(s) 826
XceI RCATGY 2 cut(s) 246, 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 731
XspI CTAG 2 cut(s) 674, 827
ZrmI AGTACT 1 cut(s) 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.