Rh2BG361700

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001297
Physical Location & Seq
Reverse (-)
50067012 .. 50129464
62453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG361700.1

Sequence Viewer

Length: 1104 bp
ATGGTATCATCAAAACCAGGAAGCAGCTTTGGCCCCTCTCATCATGACTCAGGAGTCAGTAGTATTGAGAAACTCATCACCGATGATTCAGTGATCGAAGTCCCCCAAAACTATGTTATCTCAGATCAGGAGTCTCCGGTCTCTGATGGGACTATCCTTGCCACAATCCCCACCATTGACATGAGACAGTTGGTCCTAATCAATCAGTCTGCAAACAAAGATGAACTTGAAAAGTTGCACTCAACTTGCAAAGAATGGGGAATTTTTCAGTTGGTTAACCATGGAGTTAGCTCTGCACTACTGGACAAAGTGAAGCAAGATTTAGAGGCATTTTTCAACCTTCCCTTGGAAGAGAAAATGAGATACAAAGTAAGGCCAGGTGATGTTGAAGGGTATGGAACTGTTGTCAAATCCCAAGATCAAAAACTCGACTGGGGTAACAGGTTGTACATGTTTACCAACCCTAGAAGGAAACCCCATCTTTTCCCAGAGCTCCCTACATCCCTGAGGAATACCTTGGAGACATACATGTTGGAATTACAAAAGATTGCCCGAACCCTTTTAGGGTTCATGGGAAAACTTCTAAAGATGAAGATGGAGGAGATGGAAGAGTTGTTTGAAGATGGTCTGCAGTCACTGAGGATGACTCACTATCCTCCATGCCCAAAACCAGAGCTAGTTGTTGGCCTCAGGCCACATTCTGATGGTAGTGGCATCACTATTCTCCACCAGGTTGATGGAGTTCAAGGCCTTCAAATTAAAAAAGATGGGGCTTGGTCCCCTGTGATATTTGACCATGGTGCCTTTGTTGTCAATGTTGGAGACATTCTAGAGATTCTAAGTGATGGGCTTTATAAAAGTATCGAGCACAGAGTGACTGTGAACTCAGAGAAAGAAAGGATTTCAATTGCAATGTTCTTCATGCCCAAACTTGAGGCAGAAATTGGACCTGTGACCACCCTGAGAGATCCTCAGAAGCCACCATCTTCTGGGAAAATAGGAATGGAAAAATATGTTGAGAAGTACTTTGCTCATAACCTAAATGGAAAAACACCCTTGGAGCGAATGAAAACAGAAGACAGTGAAGAGAAAACTACTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

367

Amino Acids

41.3

Weight (kDa)

5.73

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 56 - 159 5.9e-28 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 211 - 309 1.6e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 855
AasI GACNNNNNNGTC 1 cut(s) 53
AccB1I GGYRCC 1 cut(s) 800
AccB7I CCANNNNNTGG 1 cut(s) 989
AclWI GGATC 1 cut(s) 962
AcsI RAATTY 1 cut(s) 261
AdeI CACNNNGTG 1 cut(s) 874
AfaI GTAC 2 cut(s) 449, 1025
AfiI CCNNNNNNNGG 3 cut(s) 346, 564, 989
AflIII ACRYGT 2 cut(s) 450, 528
AgsI TTSAA 7 cut(s) 230, 337, 389, 620, 746, 755, 906
AhdI GACNNNNNGTC 1 cut(s) 191
AjnI CCWGG 3 cut(s) 16, 376, 729
AluBI AGCT 4 cut(s) 27, 291, 493, 676
AluI AGCT 4 cut(s) 27, 291, 493, 676
Alw21I GWGCWC 2 cut(s) 495, 870
Alw26I GTCTC 5 cut(s) 138, 145, 178, 515, 816
AlwI GGATC 1 cut(s) 962
AlwNI CAGNNNCTG 1 cut(s) 637
AoxI GGCC 5 cut(s) 31, 374, 685, 692, 748
ApeKI GCWGC 1 cut(s) 24
ApoI RAATTY 1 cut(s) 261
AspS9I GGNCC 4 cut(s) 32, 193, 777, 947
AsuHPI GGTGA 2 cut(s) 70, 392
AvaII GGWCC 3 cut(s) 193, 777, 947
AxyI CCTNAGG 2 cut(s) 506, 689
BanI GGYRCC 1 cut(s) 800
BanII GRGCYC 1 cut(s) 495
BbsI GAAGAC 1 cut(s) 1083
Bbv12I GWGCWC 2 cut(s) 495, 870
BbvI GCAGC 1 cut(s) 36
BciT130I CCWGG 3 cut(s) 18, 378, 731
BcoDI GTCTC 5 cut(s) 138, 145, 178, 515, 816
BfaI CTAG 3 cut(s) 465, 677, 830
BfmI CTRYAG 1 cut(s) 629
BisI GCNGC 1 cut(s) 25
BlsI GCNGC 1 cut(s) 26
BmcAI AGTACT 1 cut(s) 1025
Bme1390I CCNGG 3 cut(s) 18, 378, 731
Bme18I GGWCC 3 cut(s) 193, 777, 947
BmeRI GACNNNNNGTC 1 cut(s) 191
BmgT120I GGNCC 4 cut(s) 32, 193, 777, 947
BmiI GGNNCC 3 cut(s) 34, 779, 802
BmrFI CCNGG 3 cut(s) 18, 378, 731
BmrI ACTGGG 1 cut(s) 442
BmsI GCATC 1 cut(s) 723
BmuI ACTGGG 1 cut(s) 442
BpiI GAAGAC 1 cut(s) 1083
BplI GAGNNNNNCTC 4 cut(s) 631, 663, 955, 987
BpuEI CTTGAG 1 cut(s) 953
BsaI GGTCTC 1 cut(s) 145
BsaJI CCNNGG 5 cut(s) 280, 345, 516, 796, 1056
BsaWI WCCGGW 1 cut(s) 136
Bsc4I CCNNNNNNNGG 3 cut(s) 346, 564, 989
Bse1I ACTGG 2 cut(s) 306, 437
Bse21I CCTNAGG 2 cut(s) 506, 689
Bse3DI GCAATG 1 cut(s) 918
BseBI CCWGG 3 cut(s) 18, 378, 731
BseDI CCNNGG 5 cut(s) 280, 345, 516, 796, 1056
BseGI GGATG 2 cut(s) 500, 648
BseLI CCNNNNNNNGG 3 cut(s) 346, 564, 989
BseMI GCAATG 1 cut(s) 918
BseMII CTCAG 8 cut(s) 63, 135, 497, 629, 703, 900, 953, 986
BseNI ACTGG 2 cut(s) 306, 437
BseRI GAGGAG 1 cut(s) 614
BseXI GCAGC 1 cut(s) 36
BsgI GTGCAG 1 cut(s) 279
BshFI GGCC 5 cut(s) 33, 376, 687, 694, 750
BshNI GGYRCC 1 cut(s) 800
BsiHKAI GWGCWC 2 cut(s) 495, 870
BsiSI CCGG 1 cut(s) 137
BslFI GGGAC 3 cut(s) 86, 163, 763
BslI CCNNNNNNNGG 3 cut(s) 346, 564, 989
BsmAI GTCTC 5 cut(s) 138, 145, 178, 515, 816
BsmFI GGGAC 3 cut(s) 86, 163, 763
BsnI GGCC 5 cut(s) 33, 376, 687, 694, 750
Bso31I GGTCTC 1 cut(s) 145
Bsp1286I GDGCHC 2 cut(s) 495, 870
Bsp1407I TGTACA 1 cut(s) 447
Bsp143I GATC 4 cut(s) 93, 124, 418, 967
Bsp19I CCATGG 2 cut(s) 280, 796
BspANI GGCC 5 cut(s) 33, 376, 687, 694, 750
BspCNI CTCAG 8 cut(s) 62, 134, 498, 630, 702, 899, 954, 985
BspHI TCATGA 1 cut(s) 43
BspLI GGNNCC 3 cut(s) 34, 779, 802
BspMAI CTGCAG 1 cut(s) 633
BspPI GGATC 1 cut(s) 962
BspT107I GGYRCC 1 cut(s) 800
BspTNI GGTCTC 1 cut(s) 145
BsrDI GCAATG 1 cut(s) 918
BsrGI TGTACA 1 cut(s) 447
BsrI ACTGG 2 cut(s) 306, 437
BssECI CCNNGG 5 cut(s) 280, 345, 516, 796, 1056
BssMI GATC 4 cut(s) 93, 124, 418, 967
BssT1I CCWWGG 5 cut(s) 280, 345, 516, 796, 1056
Bst2UI CCWGG 3 cut(s) 18, 378, 731
Bst4CI ACNGT 4 cut(s) 189, 403, 880, 1082
Bst6I CTCTTC 3 cut(s) 345, 603, 1080
BstAUI TGTACA 1 cut(s) 447
BstDEI CTNAG 9 cut(s) 49, 121, 506, 638, 689, 839, 886, 962, 972
BstDSI CCRYGG 2 cut(s) 280, 796
BstF5I GGATG 2 cut(s) 500, 648
BstKTI GATC 4 cut(s) 96, 127, 421, 970
BstMAI GTCTC 5 cut(s) 138, 145, 178, 515, 816
BstMBI GATC 4 cut(s) 93, 124, 418, 967
BstMWI GCNNNNNNNGC 1 cut(s) 30
BstNI CCWGG 3 cut(s) 18, 378, 731
BstNSI RCATGY 2 cut(s) 454, 532
BstSCI CCNGG 3 cut(s) 16, 376, 729
BstSFI CTRYAG 1 cut(s) 629
BstV1I GCAGC 1 cut(s) 36
BstV2I GAAGAC 1 cut(s) 1083
BstX2I RGATCY 1 cut(s) 967
BstXI CCANNNNNNTGG 1 cut(s) 737
BstYI RGATCY 1 cut(s) 967
Bsu36I CCTNAGG 2 cut(s) 506, 689
BsuRI GGCC 5 cut(s) 33, 376, 687, 694, 750
BtgI CCRYGG 2 cut(s) 280, 796
BtsCI GGATG 2 cut(s) 500, 648
BtsIMutI CAGTG 3 cut(s) 96, 635, 1087
CaiI CAGNNNCTG 1 cut(s) 637
CciI TCATGA 1 cut(s) 43
Cfr13I GGNCC 4 cut(s) 32, 193, 777, 947
CsiI ACCWGGT 1 cut(s) 729
Csp6I GTAC 2 cut(s) 448, 1024
CviQI GTAC 2 cut(s) 448, 1024
DdeI CTNAG 9 cut(s) 49, 121, 506, 638, 689, 839, 886, 962, 972
DpnI GATC 4 cut(s) 95, 126, 420, 969
DpnII GATC 4 cut(s) 93, 124, 418, 967
DraIII CACNNNGTG 1 cut(s) 874
DrdI GACNNNNNNGTC 1 cut(s) 53
DriI GACNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 53
Eam1104I CTCTTC 3 cut(s) 345, 603, 1080
Eam1105I GACNNNNNGTC 1 cut(s) 191
EarI CTCTTC 3 cut(s) 345, 603, 1080
Ecl136II GAGCTC 1 cut(s) 493
Eco130I CCWWGG 5 cut(s) 280, 345, 516, 796, 1056
Eco147I AGGCCT 1 cut(s) 750
Eco24I GRGCYC 1 cut(s) 495
Eco31I GGTCTC 1 cut(s) 145
Eco47I GGWCC 3 cut(s) 193, 777, 947
Eco53kI GAGCTC 1 cut(s) 493
Eco81I CCTNAGG 2 cut(s) 506, 689
EcoICRI GAGCTC 1 cut(s) 493
EcoRII CCWGG 3 cut(s) 16, 376, 729
EcoT14I CCWWGG 5 cut(s) 280, 345, 516, 796, 1056
EcoT38I GRGCYC 1 cut(s) 495
ErhI CCWWGG 5 cut(s) 280, 345, 516, 796, 1056
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FaqI GGGAC 3 cut(s) 86, 163, 763
Fnu4HI GCNGC 1 cut(s) 25
FokI GGATG 2 cut(s) 487, 655
FriOI GRGCYC 1 cut(s) 495
Fsp4HI GCNGC 1 cut(s) 25
FspBI CTAG 3 cut(s) 465, 677, 830
GluI GCNGC 1 cut(s) 25
HaeIII GGCC 5 cut(s) 33, 376, 687, 694, 750
HapII CCGG 1 cut(s) 137
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 6 cut(s) 47, 54, 86, 131, 646, 835
HpaI GTTAAC 1 cut(s) 277
HpaII CCGG 1 cut(s) 137
HphI GGTGA 2 cut(s) 70, 392
Hpy166II GTNNAC 3 cut(s) 277, 456, 883
Hpy188I TCNGA 5 cut(s) 124, 145, 703, 889, 975
Hpy188III TCNNGA 4 cut(s) 44, 51, 128, 830
Hpy8I GTNNAC 3 cut(s) 277, 456, 883
HpyAV CCTTC 4 cut(s) 350, 383, 462, 761
HpyCH4III ACNGT 4 cut(s) 189, 403, 880, 1082
HpyCH4V TGCA 7 cut(s) 212, 238, 249, 296, 631, 911, 1100
HpyF10VI GCNNNNNNNGC 1 cut(s) 30
HpyF3I CTNAG 9 cut(s) 49, 121, 506, 638, 689, 839, 886, 962, 972
KspAI GTTAAC 1 cut(s) 277
Kzo9I GATC 4 cut(s) 93, 124, 418, 967
LmnI GCTCC 2 cut(s) 498, 1060
Lsp1109I GCAGC 1 cut(s) 36
LweI GCATC 1 cut(s) 723
MabI ACCWGGT 1 cut(s) 729
MaeI CTAG 3 cut(s) 465, 677, 830
MaeIII GTNAC 4 cut(s) 437, 633, 874, 952
MalI GATC 4 cut(s) 95, 126, 420, 969
MboI GATC 4 cut(s) 93, 124, 418, 967
MboII GAAGA 8 cut(s) 362, 604, 620, 632, 910, 978, 1088, 1097
MfeI CAATTG 1 cut(s) 906
MflI RGATCY 1 cut(s) 967
MhlI GDGCHC 2 cut(s) 495, 870
MluCI AATT 5 cut(s) 261, 536, 756, 906, 942
MlyI GAGTC 4 cut(s) 41, 63, 140, 640
MmeI TCCRAC 2 cut(s) 513, 799
MnlI CCTC 9 cut(s) 46, 319, 501, 592, 633, 666, 698, 928, 981
MseI TTAA 2 cut(s) 276, 759
MslI CAYNNNNRTG 2 cut(s) 179, 702
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 3 cut(s) 18, 378, 731
MunI CAATTG 1 cut(s) 906
MvaI CCWGG 3 cut(s) 18, 378, 731
MwoI GCNNNNNNNGC 1 cut(s) 30
NcoI CCATGG 2 cut(s) 280, 796
NdeII GATC 4 cut(s) 93, 124, 418, 967
NlaIV GGNNCC 3 cut(s) 34, 779, 802
NmuCI GTSAC 3 cut(s) 633, 874, 952
NspI RCATGY 2 cut(s) 454, 532
PagI TCATGA 1 cut(s) 43
PceI AGGCCT 1 cut(s) 750
PciI ACATGT 2 cut(s) 450, 528
PfeI GAWTC 2 cut(s) 86, 835
PflMI CCANNNNNTGG 1 cut(s) 989
PkrI GCNGC 1 cut(s) 26
PleI GAGTC 4 cut(s) 41, 62, 139, 640
PpsI GAGTC 4 cut(s) 41, 62, 139, 640
PscI ACATGT 2 cut(s) 450, 528
PsiI TTATAA 1 cut(s) 855
Psp124BI GAGCTC 1 cut(s) 495
Psp6I CCWGG 3 cut(s) 16, 376, 729
PspGI CCWGG 3 cut(s) 16, 376, 729
PspN4I GGNNCC 3 cut(s) 34, 779, 802
PspPI GGNCC 4 cut(s) 32, 193, 777, 947
PstI CTGCAG 1 cut(s) 633
PstNI CAGNNNCTG 1 cut(s) 637
PsuI RGATCY 1 cut(s) 967
RsaI GTAC 2 cut(s) 449, 1025
RsaNI GTAC 2 cut(s) 448, 1024
RseI CAYNNNNRTG 2 cut(s) 179, 702
SacI GAGCTC 1 cut(s) 495
SaqAI TTAA 2 cut(s) 276, 759
SatI GCNGC 1 cut(s) 25
Sau3AI GATC 4 cut(s) 93, 124, 418, 967
Sau96I GGNCC 4 cut(s) 32, 193, 777, 947
ScaI AGTACT 1 cut(s) 1025
SchI GAGTC 4 cut(s) 41, 63, 140, 640
ScrFI CCNGG 3 cut(s) 18, 378, 731
SduI GDGCHC 2 cut(s) 495, 870
SexAI ACCWGGT 1 cut(s) 729
SfaNI GCATC 1 cut(s) 723
SfcI CTRYAG 1 cut(s) 629
SinI GGWCC 3 cut(s) 193, 777, 947
SmiMI CAYNNNNRTG 2 cut(s) 179, 702
SmlI CTYRAG 1 cut(s) 932
SmoI CTYRAG 1 cut(s) 932
Sse9I AATT 5 cut(s) 261, 536, 756, 906, 942
SseBI AGGCCT 1 cut(s) 750
SspMI CTAG 3 cut(s) 465, 677, 830
SstI GAGCTC 1 cut(s) 495
StuI AGGCCT 1 cut(s) 750
StyD4I CCNGG 3 cut(s) 16, 376, 729
StyI CCWWGG 5 cut(s) 280, 345, 516, 796, 1056
TaaI ACNGT 4 cut(s) 189, 403, 880, 1082
TaqI TCGA 3 cut(s) 96, 429, 864
TasI AATT 5 cut(s) 261, 536, 756, 906, 942
TatI WGTACW 2 cut(s) 447, 1023
TfiI GAWTC 2 cut(s) 86, 835
Tru1I TTAA 2 cut(s) 276, 759
Tru9I TTAA 2 cut(s) 276, 759
TscAI CASTG 3 cut(s) 96, 642, 1087
TseFI GTSAC 3 cut(s) 633, 874, 952
TseI GCWGC 1 cut(s) 24
Tsp45I GTSAC 3 cut(s) 633, 874, 952
TspDTI ATGAA 5 cut(s) 237, 559, 605, 910, 1082
TspRI CASTG 3 cut(s) 96, 642, 1087
Van91I CCANNNNNTGG 1 cut(s) 989
VpaK11BI GGWCC 3 cut(s) 193, 777, 947
XapI RAATTY 1 cut(s) 261
XbaI TCTAGA 1 cut(s) 829
XceI RCATGY 2 cut(s) 454, 532
XcmI CCANNNNNNNNNTGG 1 cut(s) 734
XspI CTAG 3 cut(s) 465, 677, 830
ZrmI AGTACT 1 cut(s) 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.