RLG00000031934

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001297
Physical Location & Seq
Forward (+)
9635867 .. 9652064
16198 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031934

Sequence Viewer

Length: 1308 bp
ATGTTTCGCGACAGTGAACAGACTCAACCTTCGCGAACGGCAGACTTAGTTATTCCACGAACTAAGTTTGTGAACCAAATCTTTGATCTCTCAAAGCGTCCAAAAAGCGAGGCGAGAGCTACGATTATGTCCATGTGCAAGACTATGGGAGCTGCGGTGGTAGTTTTCTGCCACGTTTGCCTAGTGAAGAAAGAGCTCTCAATGGTTGCGGCATTGGCTTTTCTGTTCAACCAAGGATTATTGAAATCAGGTATCATGACGGGCCCCGCAAAAGACGAGAAATTGGCCAAAATTGTTGAATCCCTAGTCAGGCATGATAGAATTAATGCTTTCCTGTTCCATCCGCGAAAAGTTCATGAAGATTCTCAAAAAAAGCTAGGCGAAAGCTACAATTATGTCCACGTGCAAGACTATGGGAGCTGCGGTGGTAGTTTTCGGCCTCGTTTGCCTAGTGAAGAAAGAGCTCTCAGTGGTTGCGGCATTGGCTTTTCTGTTCAACTAAGGATTATTGAAATCAGGTATTATGACGGGCCCCGCAAAAGACGAGTTGGGAACCATGGAGTTAGCCCTATGCTTTTGGAGAAGCTGAAGTACGAGGTTGAAGAATTTTTTAAGCTTCCCTTGGAAGAGAAAATGAAGTATCAGAGAAGGCCTGGTGACGTTGAAGAGCAAAAACGTGACTGGGGTAACAGATTCTACATGACCATGAACCCTTCATACTACTTGGAACTGAAGCAACTAGCCATGGAACTTCTTGGGTTTCTGGGGAAGGCTGTGAAGATAGACATAAAAGAGATTGAGGAGATGTTTGAAGATGGGATGCAGTCAGTGAGGATGAATTACTATCCTGCATGCCCACAACCAGAGCTGGTCATCGGCCTCACACCTCACTCGGACGCTTCCGGGATCACCATCCTCTACCAGCTTAACGGTGTGGGTGGTCTGCAGATTAAGAAAGATGGGGTTTGGGTTCCTGTCAACTTCCGTGAAGATGCCTTTGTGGTTAACGCAGGGGACATCTTTGAGTATGAATTAGTATATCCACGTGTACATCTAATTATAGATCCTGAGCAATGGAGCGTACAAGAGCATGGAGCACAGAGCTATGATGAACCCTCGATCTCGATCGCTATGTTCTTCAACCCCAAATTCGAGGCAGAAATTGGACCATTGACCAGCTTGATAACCCCAGAAAATCCACCACTGTTCAAAAGGATTGGGATGGAACAGTACGTCCAAGATTTCTACACCCGTAAGGTCGACGGGAAATCATATTTGGAGCAAATGGGGCTCCAGAGTGGGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

436

Amino Acids

49.74

Weight (kDa)

6.78

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 183 - 230 2.9e-08 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 275 - 343 2.3e-20 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1260
AccII CGCG 3 cut(s) 9, 34, 346
AciI CCGC 7 cut(s) 155, 209, 267, 344, 423, 477, 535
AclWI GGATC 2 cut(s) 914, 1058
AcoI YGGCCR 1 cut(s) 285
AcsI RAATTY 2 cut(s) 605, 1148
AcuI CTGAAG 2 cut(s) 608, 752
AcvI CACGTG 2 cut(s) 403, 1046
AfaI GTAC 4 cut(s) 593, 1050, 1083, 1232
AfiI CCNNNNNNNGG 1 cut(s) 309
AflIII ACRYGT 1 cut(s) 1045
AjnI CCWGG 1 cut(s) 652
AjuI GAANNNNNNNTTGG 2 cut(s) 1259, 1291
AloI GAACNNNNNNTCC 2 cut(s) 1218, 1250
Alw21I GWGCWC 3 cut(s) 198, 466, 1099
AlwI GGATC 2 cut(s) 914, 1058
AoxI GGCC 6 cut(s) 262, 285, 437, 530, 650, 877
ApaI GGGCCC 2 cut(s) 266, 534
ApeKI GCWGC 2 cut(s) 152, 420
ApoI RAATTY 2 cut(s) 605, 1148
ArsI GACNNNNNNTTYG 2 cut(s) 13, 45
AseI ATTAAT 1 cut(s) 324
AspS9I GGNCC 5 cut(s) 262, 263, 530, 531, 1166
AsuC2I CCSGG 1 cut(s) 904
AsuHPI GGTGA 2 cut(s) 668, 901
AvaII GGWCC 1 cut(s) 1166
BaeGI GKGCMC 2 cut(s) 266, 534
BalI TGGCCA 1 cut(s) 287
BanII GRGCYC 5 cut(s) 198, 266, 466, 534, 1293
BarI GAAGNNNNNNTAC 2 cut(s) 575, 607
BbrPI CACGTG 2 cut(s) 403, 1046
Bbv12I GWGCWC 3 cut(s) 198, 466, 1099
BbvI GCAGC 2 cut(s) 139, 407
BccI CCATC 5 cut(s) 348, 809, 920, 953, 1216
BceAI ACGGC 1 cut(s) 54
BciT130I CCWGG 1 cut(s) 654
BcnI CCSGG 1 cut(s) 904
BfaI CTAG 5 cut(s) 182, 305, 377, 450, 740
BfmI CTRYAG 1 cut(s) 944
BisI GCNGC 4 cut(s) 153, 210, 421, 478
BlsI GCNGC 4 cut(s) 154, 211, 422, 479
Bme1390I CCNGG 2 cut(s) 654, 904
Bme18I GGWCC 1 cut(s) 1166
BmgT120I GGNCC 5 cut(s) 262, 263, 530, 531, 1166
BmiI GGNNCC 7 cut(s) 264, 265, 532, 533, 554, 972, 1292
BmrFI CCNGG 2 cut(s) 654, 904
BmrI ACTGGG 1 cut(s) 691
BmsI GCATC 2 cut(s) 810, 982
BmuI ACTGGG 1 cut(s) 691
BpmI CTGGAG 1 cut(s) 1277
Bpu10I CCTNAGC 1 cut(s) 1068
BpuMI CCSGG 1 cut(s) 904
BsaAI YACGTR 2 cut(s) 403, 1046
BsaBI GATNNNNATC 2 cut(s) 911, 1124
BsaJI CCNNGG 4 cut(s) 232, 556, 621, 744
BsaXI ACNNNNNCTCC 4 cut(s) 141, 171, 409, 439
Bsc4I CCNNNNNNNGG 1 cut(s) 309
Bse1I ACTGG 1 cut(s) 686
Bse3DI GCAATG 1 cut(s) 1079
Bse8I GATNNNNATC 2 cut(s) 911, 1124
BseBI CCWGG 1 cut(s) 654
BseDI CCNNGG 4 cut(s) 232, 556, 621, 744
BseGI GGATG 5 cut(s) 340, 825, 840, 912, 1227
BseJI GATNNNNATC 2 cut(s) 911, 1124
BseLI CCNNNNNNNGG 1 cut(s) 309
BseMI GCAATG 1 cut(s) 1079
BseMII CTCAG 2 cut(s) 481, 1059
BseNI ACTGG 1 cut(s) 686
BseRI GAGGAG 1 cut(s) 815
BseSI GKGCMC 2 cut(s) 266, 534
BseXI GCAGC 2 cut(s) 139, 407
Bsh1236I CGCG 3 cut(s) 9, 34, 346
Bsh1285I CGRYCG 1 cut(s) 1128
BshFI GGCC 6 cut(s) 264, 287, 439, 532, 652, 879
BsiEI CGRYCG 1 cut(s) 1128
BsiHKAI GWGCWC 3 cut(s) 198, 466, 1099
BsiSI CCGG 1 cut(s) 903
BslFI GGGAC 1 cut(s) 1028
BslI CCNNNNNNNGG 1 cut(s) 309
BsmFI GGGAC 1 cut(s) 1028
BsnI GGCC 6 cut(s) 264, 287, 439, 532, 652, 879
Bsp120I GGGCCC 2 cut(s) 262, 530
Bsp1286I GDGCHC 6 cut(s) 198, 266, 466, 534, 1099, 1293
Bsp1407I TGTACA 1 cut(s) 1048
Bsp143I GATC 5 cut(s) 85, 906, 1063, 1119, 1125
Bsp19I CCATGG 2 cut(s) 556, 744
Bsp68I TCGCGA 2 cut(s) 9, 34
BspACI CCGC 7 cut(s) 155, 209, 267, 344, 423, 477, 535
BspANI GGCC 6 cut(s) 264, 287, 439, 532, 652, 879
BspCNI CTCAG 2 cut(s) 480, 1060
BspFNI CGCG 3 cut(s) 9, 34, 346
BspHI TCATGA 2 cut(s) 255, 355
BspLI GGNNCC 7 cut(s) 264, 265, 532, 533, 554, 972, 1292
BspMAI CTGCAG 1 cut(s) 948
BspPI GGATC 2 cut(s) 914, 1058
BspQI GCTCTTC 1 cut(s) 660
BsrDI GCAATG 1 cut(s) 1079
BsrGI TGTACA 1 cut(s) 1048
BsrI ACTGG 1 cut(s) 686
BssECI CCNNGG 4 cut(s) 232, 556, 621, 744
BssMI GATC 5 cut(s) 85, 906, 1063, 1119, 1125
BssT1I CCWWGG 4 cut(s) 232, 556, 621, 744
Bst2UI CCWGG 1 cut(s) 654
Bst4CI ACNGT 4 cut(s) 14, 932, 1206, 1230
Bst6I CTCTTC 2 cut(s) 621, 660
BstAUI TGTACA 1 cut(s) 1048
BstBAI YACGTR 2 cut(s) 403, 1046
BstC8I GCNNGC 1 cut(s) 853
BstDEI CTNAG 5 cut(s) 46, 63, 467, 500, 1068
BstDSI CCRYGG 2 cut(s) 556, 744
BstF5I GGATG 5 cut(s) 340, 825, 840, 912, 1227
BstFNI CGCG 3 cut(s) 9, 34, 346
BstKTI GATC 5 cut(s) 88, 909, 1066, 1122, 1128
BstMBI GATC 5 cut(s) 85, 906, 1063, 1119, 1125
BstMCI CGRYCG 1 cut(s) 1128
BstMWI GCNNNNNNNGC 5 cut(s) 177, 215, 445, 483, 1288
BstNI CCWGG 1 cut(s) 654
BstNSI RCATGY 1 cut(s) 855
BstSCI CCNGG 2 cut(s) 652, 902
BstSFI CTRYAG 1 cut(s) 944
BstSLI GKGCMC 2 cut(s) 266, 534
BstUI CGCG 3 cut(s) 9, 34, 346
BstV1I GCAGC 2 cut(s) 139, 407
BstX2I RGATCY 1 cut(s) 1063
BstYI RGATCY 1 cut(s) 1063
BsuRI GGCC 6 cut(s) 264, 287, 439, 532, 652, 879
BtgI CCRYGG 2 cut(s) 556, 744
BtsCI GGATG 5 cut(s) 340, 825, 840, 912, 1227
BtsIMutI CAGTG 4 cut(s) 19, 475, 834, 1202
BtuMI TCGCGA 2 cut(s) 9, 34
Cac8I GCNNGC 1 cut(s) 853
CciI TCATGA 2 cut(s) 255, 355
Cfr13I GGNCC 5 cut(s) 262, 263, 530, 531, 1166
CseI GACGC 2 cut(s) 86, 905
Csp6I GTAC 4 cut(s) 592, 1049, 1082, 1231
CviQI GTAC 4 cut(s) 592, 1049, 1082, 1231
DdeI CTNAG 5 cut(s) 46, 63, 467, 500, 1068
DpnI GATC 5 cut(s) 87, 908, 1065, 1121, 1127
DpnII GATC 5 cut(s) 85, 906, 1063, 1119, 1125
EaeI YGGCCR 1 cut(s) 285
Eam1104I CTCTTC 2 cut(s) 621, 660
EarI CTCTTC 2 cut(s) 621, 660
Ecl136II GAGCTC 2 cut(s) 196, 464
Eco130I CCWWGG 4 cut(s) 232, 556, 621, 744
Eco147I AGGCCT 1 cut(s) 652
Eco24I GRGCYC 5 cut(s) 198, 266, 466, 534, 1293
Eco47I GGWCC 1 cut(s) 1166
Eco53kI GAGCTC 2 cut(s) 196, 464
Eco57I CTGAAG 2 cut(s) 608, 752
Eco72I CACGTG 2 cut(s) 403, 1046
EcoICRI GAGCTC 2 cut(s) 196, 464
EcoO109I RGGNCCY 2 cut(s) 263, 531
EcoRII CCWGG 1 cut(s) 652
EcoT14I CCWWGG 4 cut(s) 232, 556, 621, 744
EcoT38I GRGCYC 5 cut(s) 198, 266, 466, 534, 1293
ErhI CCWWGG 4 cut(s) 232, 556, 621, 744
FalI AAGNNNNNCTT 2 cut(s) 605, 637
FaqI GGGAC 1 cut(s) 1028
FauI CCCGC 2 cut(s) 274, 542
FblI GTMKAC 1 cut(s) 1260
Fnu4HI GCNGC 4 cut(s) 153, 210, 421, 478
FokI GGATG 5 cut(s) 327, 832, 847, 899, 1234
FriOI GRGCYC 5 cut(s) 198, 266, 466, 534, 1293
Fsp4HI GCNGC 4 cut(s) 153, 210, 421, 478
FspBI CTAG 5 cut(s) 182, 305, 377, 450, 740
GluI GCNGC 4 cut(s) 153, 210, 421, 478
GsuI CTGGAG 1 cut(s) 1277
HaeIII GGCC 6 cut(s) 264, 287, 439, 532, 652, 879
HapII CCGG 1 cut(s) 903
HgaI GACGC 2 cut(s) 86, 905
HincII GTYRAC 3 cut(s) 979, 1006, 1261
HindII GTYRAC 3 cut(s) 979, 1006, 1261
HindIII AAGCTT 1 cut(s) 614
HinfI GANTC 4 cut(s) 22, 299, 362, 693
HpaI GTTAAC 1 cut(s) 1006
HpaII CCGG 1 cut(s) 903
HphI GGTGA 2 cut(s) 668, 901
Hpy166II GTNNAC 7 cut(s) 17, 73, 400, 979, 1006, 1049, 1261
Hpy188I TCNGA 2 cut(s) 645, 895
Hpy188III TCNNGA 7 cut(s) 8, 33, 256, 356, 1067, 1123, 1294
Hpy8I GTNNAC 7 cut(s) 17, 73, 400, 979, 1006, 1049, 1261
Hpy99I CGWCG 1 cut(s) 1265
HpyAV CCTTC 4 cut(s) 39, 642, 723, 763
HpyCH4III ACNGT 4 cut(s) 14, 932, 1206, 1230
HpyCH4IV ACGT 6 cut(s) 174, 402, 660, 676, 1045, 1233
HpyCH4V TGCA 5 cut(s) 138, 406, 823, 851, 946
HpyF10VI GCNNNNNNNGC 5 cut(s) 177, 215, 445, 483, 1288
HpyF3I CTNAG 5 cut(s) 46, 63, 467, 500, 1068
HpySE526I ACGT 6 cut(s) 174, 402, 660, 676, 1045, 1233
KspAI GTTAAC 1 cut(s) 1006
Kzo9I GATC 5 cut(s) 85, 906, 1063, 1119, 1125
LguI GCTCTTC 1 cut(s) 660
LmnI GCTCC 6 cut(s) 149, 417, 1077, 1094, 1279, 1296
Lsp1109I GCAGC 2 cut(s) 139, 407
LweI GCATC 2 cut(s) 810, 982
MaeI CTAG 5 cut(s) 182, 305, 377, 450, 740
MaeII ACGT 6 cut(s) 174, 402, 660, 676, 1045, 1233
MaeIII GTNAC 3 cut(s) 656, 677, 686
MalI GATC 5 cut(s) 87, 908, 1065, 1121, 1127
MboI GATC 5 cut(s) 85, 906, 1063, 1119, 1125
MflI RGATCY 1 cut(s) 1063
MhlI GDGCHC 6 cut(s) 198, 266, 466, 534, 1099, 1293
MlsI TGGCCA 1 cut(s) 287
MluNI TGGCCA 1 cut(s) 287
MlyI GAGTC 1 cut(s) 16
Mox20I TGGCCA 1 cut(s) 287
MscI TGGCCA 1 cut(s) 287
MseI TTAA 5 cut(s) 324, 612, 927, 951, 1005
MslI CAYNNNNRTG 1 cut(s) 704
Msp20I TGGCCA 1 cut(s) 287
MspI CCGG 1 cut(s) 903
MspR9I CCNGG 2 cut(s) 654, 904
MvaI CCWGG 1 cut(s) 654
MvnI CGCG 3 cut(s) 9, 34, 346
MwoI GCNNNNNNNGC 5 cut(s) 177, 215, 445, 483, 1288
NciI CCSGG 1 cut(s) 904
NcoI CCATGG 2 cut(s) 556, 744
NdeII GATC 5 cut(s) 85, 906, 1063, 1119, 1125
NlaIV GGNNCC 7 cut(s) 264, 265, 532, 533, 554, 972, 1292
NmuCI GTSAC 2 cut(s) 656, 677
NruI TCGCGA 2 cut(s) 9, 34
NspI RCATGY 1 cut(s) 855
PaeI GCATGC 1 cut(s) 855
PagI TCATGA 2 cut(s) 255, 355
PceI AGGCCT 1 cut(s) 652
PciSI GCTCTTC 1 cut(s) 660
PfeI GAWTC 3 cut(s) 299, 362, 693
PfoI TCCNGGA 1 cut(s) 902
PkrI GCNGC 4 cut(s) 154, 211, 422, 479
Ple19I CGATCG 1 cut(s) 1128
PleI GAGTC 1 cut(s) 16
PmaCI CACGTG 2 cut(s) 403, 1046
PmlI CACGTG 2 cut(s) 403, 1046
PpsI GAGTC 1 cut(s) 16
Ppu21I YACGTR 2 cut(s) 403, 1046
PshBI ATTAAT 1 cut(s) 324
Psp124BI GAGCTC 2 cut(s) 198, 466
Psp6I CCWGG 1 cut(s) 652
PspCI CACGTG 2 cut(s) 403, 1046
PspGI CCWGG 1 cut(s) 652
PspN4I GGNNCC 7 cut(s) 264, 265, 532, 533, 554, 972, 1292
PspOMI GGGCCC 2 cut(s) 262, 530
PspPI GGNCC 5 cut(s) 262, 263, 530, 531, 1166
PsrI GAACNNNNNNTAC 2 cut(s) 701, 733
PstI CTGCAG 1 cut(s) 948
PsuI RGATCY 1 cut(s) 1063
PvuI CGATCG 1 cut(s) 1128
RruI TCGCGA 2 cut(s) 9, 34
RsaI GTAC 4 cut(s) 593, 1050, 1083, 1232
RsaNI GTAC 4 cut(s) 592, 1049, 1082, 1231
RseI CAYNNNNRTG 1 cut(s) 704
SacI GAGCTC 2 cut(s) 198, 466
SalI GTCGAC 1 cut(s) 1259
SapI GCTCTTC 1 cut(s) 660
SaqAI TTAA 5 cut(s) 324, 612, 927, 951, 1005
SatI GCNGC 4 cut(s) 153, 210, 421, 478
Sau3AI GATC 5 cut(s) 85, 906, 1063, 1119, 1125
Sau96I GGNCC 5 cut(s) 262, 263, 530, 531, 1166
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 654, 904
SduI GDGCHC 6 cut(s) 198, 266, 466, 534, 1099, 1293
SfaNI GCATC 2 cut(s) 810, 982
SfcI CTRYAG 1 cut(s) 944
SinI GGWCC 1 cut(s) 1166
SmiMI CAYNNNNRTG 1 cut(s) 704
SphI GCATGC 1 cut(s) 855
SseBI AGGCCT 1 cut(s) 652
SsiI CCGC 7 cut(s) 155, 209, 267, 344, 423, 477, 535
SspMI CTAG 5 cut(s) 182, 305, 377, 450, 740
SstI GAGCTC 2 cut(s) 198, 466
StuI AGGCCT 1 cut(s) 652
StyD4I CCNGG 2 cut(s) 652, 902
StyI CCWWGG 4 cut(s) 232, 556, 621, 744
TaaI ACNGT 4 cut(s) 14, 932, 1206, 1230
TaiI ACGT 6 cut(s) 177, 405, 663, 679, 1048, 1236
TaqI TCGA 4 cut(s) 1118, 1124, 1152, 1260
TatI WGTACW 1 cut(s) 1048
TauI GCSGC 2 cut(s) 212, 480
TfiI GAWTC 3 cut(s) 299, 362, 693
Tru1I TTAA 5 cut(s) 324, 612, 927, 951, 1005
Tru9I TTAA 5 cut(s) 324, 612, 927, 951, 1005
TscAI CASTG 4 cut(s) 19, 475, 834, 1209
TseFI GTSAC 2 cut(s) 656, 677
TseI GCWGC 2 cut(s) 152, 420
Tsp45I GTSAC 2 cut(s) 656, 677
TspDTI ATGAA 8 cut(s) 344, 372, 650, 705, 722, 851, 1044, 1125
TspGWI ACGGA 1 cut(s) 974
TspRI CASTG 4 cut(s) 19, 475, 834, 1209
VpaK11BI GGWCC 1 cut(s) 1166
VspI ATTAAT 1 cut(s) 324
XapI RAATTY 2 cut(s) 605, 1148
XceI RCATGY 1 cut(s) 855
XmiI GTMKAC 1 cut(s) 1260
XspI CTAG 5 cut(s) 182, 305, 377, 450, 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.