Rw2G028880

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001297
Physical Location & Seq
Reverse (-)
46374396 .. 46377170
2775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G028880.1

Sequence Viewer

Length: 1071 bp
ATGGCATCATCAAAACCAGGAAGCAGCTTTGGCCCCTCTCATCATGACTCAGGAGTCAGTAGTATTGAGAAACTCATCACCGATGATTCAGTGATCGAAGTCCCCCAAAACTATGTTATCTCAGATCAGGAGTCTCCGGTCTCTGATGGGACCACAATCCCCACCATTGACATGAGACAGTTGGTCCTAATCAATCAGTCTGCAAACAAAGATGAACTTGAAAAGTTGCACTCAACTTGCAAAGAATGGGGAATTTTTCAGCTCTGCACTACTGGACAAAGTGAAGCAAGATTTAGAGGCATCAAGCTTCCCTTGGAAGAGAAAATGAGATACAAAGTAAGGCCAGGTGATGTTGAAGGGTATGGAACTGTTGTCAAATCCCAAGATCAAAAACTCGACTGGGGTAACAGGTTGTACATGTTTACCAACCCTAGAAGGAAACCCCATCTTTTCCCAGAGCTCCCTACATCCCTGAGGAATACCTTGGAGTGGTACATGTTGGAATTACAAAGGATTGCCCGGACCCTTTTAGGGTTCATGGGAAAACTTCTAAAGATGAAGATGGAGGAGATGGAAGAGTTGTTTCAAGATGGGCTGCAGTCACTGAGGATGACTCACTATCCTCCATGCCCACAACCAGGGCTAGTTGGTGGCCTCAGGCCTCATTCTGATGGTAGTGGCATCACTATTCTCCACCAGGTTAATGGAGTTCAAGGCCTTCAAATTAAAAAAGATGGGGCTTGGTTCCCTGTGATCTTTGACCATGGTGCCTTTGTTGTCAATGTTGGAGACATTCTAGAGATTCTGAGTGATGGGATTTATAAAAGTATTGAGCACAGAGTAATTGTGAACCCAGAGAAAGAAAGGATTTCAATTGCTATGTTCTTCATGCCCAAACTTGAGTCAGAAATTGGACCTGTGACCGCCCTGAGAGATCCTCAGAAGCCACCATCTCCTGGGAAAATAGGAATGGCAAAATTTGTCGAGAATTACTTCGCTCATAATCTTAATGGAAAAACACCCTTGGAGCGAATCAGAACAAAAGATGGTGAAGAGAATACTACTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

356

Amino Acids

40.04

Weight (kDa)

6.39

Isoelectric Point (pI)

49.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 53 - 148 1.3e-13 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 200 - 298 1.8e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 822
AasI GACNNNNNNGTC 1 cut(s) 53
AccB1I GGYRCC 1 cut(s) 767
AccB7I CCANNNNNTGG 1 cut(s) 956
AciI CCGC 1 cut(s) 924
AclWI GGATC 1 cut(s) 929
AcsI RAATTY 2 cut(s) 252, 977
AfaI GTAC 2 cut(s) 416, 494
AfiI CCNNNNNNNGG 4 cut(s) 489, 531, 638, 956
AflIII ACRYGT 2 cut(s) 417, 495
AgsI TTSAA 6 cut(s) 221, 356, 587, 713, 722, 873
AhdI GACNNNNNGTC 1 cut(s) 182
AjnI CCWGG 5 cut(s) 16, 343, 637, 696, 955
AluBI AGCT 4 cut(s) 27, 262, 307, 460
AluI AGCT 4 cut(s) 27, 262, 307, 460
Alw21I GWGCWC 2 cut(s) 462, 837
Alw26I GTCTC 4 cut(s) 138, 145, 169, 783
AlwI GGATC 1 cut(s) 929
AlwNI CAGNNNCTG 1 cut(s) 604
AoxI GGCC 5 cut(s) 31, 341, 652, 659, 715
ApeKI GCWGC 2 cut(s) 24, 595
ApoI RAATTY 2 cut(s) 252, 977
Asp700I GAANNNNTTC 1 cut(s) 992
AspS9I GGNCC 5 cut(s) 32, 150, 184, 522, 914
AsuC2I CCSGG 1 cut(s) 520
AsuHPI GGTGA 3 cut(s) 70, 359, 1061
AvaII GGWCC 4 cut(s) 150, 184, 522, 914
AxyI CCTNAGG 2 cut(s) 473, 656
BanI GGYRCC 1 cut(s) 767
BanII GRGCYC 1 cut(s) 462
Bbv12I GWGCWC 2 cut(s) 462, 837
BbvI GCAGC 2 cut(s) 36, 582
BciT130I CCWGG 5 cut(s) 18, 345, 639, 698, 957
BcnI CCSGG 1 cut(s) 520
BcoDI GTCTC 4 cut(s) 138, 145, 169, 783
BfaI CTAG 3 cut(s) 432, 644, 797
BfmI CTRYAG 1 cut(s) 596
BisI GCNGC 2 cut(s) 25, 596
BlsI GCNGC 2 cut(s) 26, 597
Bme1390I CCNGG 6 cut(s) 18, 345, 520, 639, 698, 957
Bme18I GGWCC 4 cut(s) 150, 184, 522, 914
BmeRI GACNNNNNGTC 1 cut(s) 182
BmgT120I GGNCC 5 cut(s) 32, 150, 184, 522, 914
BmiI GGNNCC 5 cut(s) 34, 151, 524, 746, 769
BmrFI CCNGG 6 cut(s) 18, 345, 520, 639, 698, 957
BmrI ACTGGG 1 cut(s) 409
BmsI GCATC 3 cut(s) 14, 309, 690
BmuI ACTGGG 1 cut(s) 409
BplI GAGNNNNNCTC 4 cut(s) 598, 630, 922, 954
BpuEI CTTGAG 1 cut(s) 920
BpuMI CCSGG 1 cut(s) 520
BsaI GGTCTC 1 cut(s) 145
BsaJI CCNNGG 6 cut(s) 312, 483, 638, 763, 956, 1023
BsaWI WCCGGW 1 cut(s) 136
Bsc4I CCNNNNNNNGG 4 cut(s) 489, 531, 638, 956
Bse1I ACTGG 2 cut(s) 277, 404
Bse21I CCTNAGG 2 cut(s) 473, 656
BseBI CCWGG 5 cut(s) 18, 345, 639, 698, 957
BseDI CCNNGG 6 cut(s) 312, 483, 638, 763, 956, 1023
BseGI GGATG 2 cut(s) 467, 615
BseLI CCNNNNNNNGG 4 cut(s) 489, 531, 638, 956
BseMII CTCAG 8 cut(s) 63, 135, 464, 596, 670, 797, 920, 953
BseNI ACTGG 2 cut(s) 277, 404
BseRI GAGGAG 1 cut(s) 581
BseXI GCAGC 2 cut(s) 36, 582
BsgI GTGCAG 1 cut(s) 250
BshFI GGCC 5 cut(s) 33, 343, 654, 661, 717
BshNI GGYRCC 1 cut(s) 767
BsiHKAI GWGCWC 2 cut(s) 462, 837
BsiSI CCGG 2 cut(s) 137, 520
BslFI GGGAC 2 cut(s) 86, 163
BslI CCNNNNNNNGG 4 cut(s) 489, 531, 638, 956
BsmAI GTCTC 4 cut(s) 138, 145, 169, 783
BsmFI GGGAC 2 cut(s) 86, 163
BsnI GGCC 5 cut(s) 33, 343, 654, 661, 717
Bso31I GGTCTC 1 cut(s) 145
Bsp1286I GDGCHC 2 cut(s) 462, 837
Bsp1407I TGTACA 1 cut(s) 414
Bsp143I GATC 5 cut(s) 93, 124, 385, 753, 934
Bsp19I CCATGG 1 cut(s) 763
BspACI CCGC 1 cut(s) 924
BspANI GGCC 5 cut(s) 33, 343, 654, 661, 717
BspCNI CTCAG 8 cut(s) 62, 134, 465, 597, 669, 798, 921, 952
BspHI TCATGA 1 cut(s) 43
BspLI GGNNCC 5 cut(s) 34, 151, 524, 746, 769
BspMAI CTGCAG 1 cut(s) 600
BspPI GGATC 1 cut(s) 929
BspT107I GGYRCC 1 cut(s) 767
BspTNI GGTCTC 1 cut(s) 145
BsrGI TGTACA 1 cut(s) 414
BsrI ACTGG 2 cut(s) 277, 404
BssECI CCNNGG 6 cut(s) 312, 483, 638, 763, 956, 1023
BssMI GATC 5 cut(s) 93, 124, 385, 753, 934
BssT1I CCWWGG 4 cut(s) 312, 483, 763, 1023
Bst2UI CCWGG 5 cut(s) 18, 345, 639, 698, 957
Bst4CI ACNGT 2 cut(s) 180, 370
Bst6I CTCTTC 3 cut(s) 312, 570, 1047
BstAUI TGTACA 1 cut(s) 414
BstDEI CTNAG 8 cut(s) 49, 121, 473, 605, 656, 806, 929, 939
BstDSI CCRYGG 1 cut(s) 763
BstF5I GGATG 2 cut(s) 467, 615
BstKTI GATC 5 cut(s) 96, 127, 388, 756, 937
BstMAI GTCTC 4 cut(s) 138, 145, 169, 783
BstMBI GATC 5 cut(s) 93, 124, 385, 753, 934
BstMWI GCNNNNNNNGC 1 cut(s) 30
BstNI CCWGG 5 cut(s) 18, 345, 639, 698, 957
BstNSI RCATGY 2 cut(s) 421, 499
BstSCI CCNGG 6 cut(s) 16, 343, 518, 637, 696, 955
BstSFI CTRYAG 1 cut(s) 596
BstV1I GCAGC 2 cut(s) 36, 582
BstX2I RGATCY 1 cut(s) 934
BstXI CCANNNNNNTGG 1 cut(s) 704
BstYI RGATCY 1 cut(s) 934
Bsu36I CCTNAGG 2 cut(s) 473, 656
BsuRI GGCC 5 cut(s) 33, 343, 654, 661, 717
BtgI CCRYGG 1 cut(s) 763
BtsCI GGATG 2 cut(s) 467, 615
BtsIMutI CAGTG 2 cut(s) 96, 602
CaiI CAGNNNCTG 1 cut(s) 604
CciI TCATGA 1 cut(s) 43
Cfr13I GGNCC 5 cut(s) 32, 150, 184, 522, 914
CsiI ACCWGGT 1 cut(s) 696
Csp6I GTAC 2 cut(s) 415, 493
CviAII CATG 9 cut(s) 44, 172, 418, 496, 538, 627, 764, 889, 1068
CviQI GTAC 2 cut(s) 415, 493
DdeI CTNAG 8 cut(s) 49, 121, 473, 605, 656, 806, 929, 939
DpnI GATC 5 cut(s) 95, 126, 387, 755, 936
DpnII GATC 5 cut(s) 93, 124, 385, 753, 934
DrdI GACNNNNNNGTC 1 cut(s) 53
DriI GACNNNNNGTC 1 cut(s) 182
DseDI GACNNNNNNGTC 1 cut(s) 53
Eam1104I CTCTTC 3 cut(s) 312, 570, 1047
Eam1105I GACNNNNNGTC 1 cut(s) 182
EarI CTCTTC 3 cut(s) 312, 570, 1047
Ecl136II GAGCTC 1 cut(s) 460
Eco130I CCWWGG 4 cut(s) 312, 483, 763, 1023
Eco147I AGGCCT 2 cut(s) 661, 717
Eco24I GRGCYC 1 cut(s) 462
Eco31I GGTCTC 1 cut(s) 145
Eco47I GGWCC 4 cut(s) 150, 184, 522, 914
Eco53kI GAGCTC 1 cut(s) 460
Eco81I CCTNAGG 2 cut(s) 473, 656
EcoICRI GAGCTC 1 cut(s) 460
EcoRII CCWGG 5 cut(s) 16, 343, 637, 696, 955
EcoT14I CCWWGG 4 cut(s) 312, 483, 763, 1023
EcoT38I GRGCYC 1 cut(s) 462
ErhI CCWWGG 4 cut(s) 312, 483, 763, 1023
FaeI CATG 9 cut(s) 47, 175, 421, 499, 541, 630, 767, 892, 1071
FalI AAGNNNNNCTT 4 cut(s) 201, 233, 296, 328
FaqI GGGAC 2 cut(s) 86, 163
FatI CATG 9 cut(s) 43, 171, 417, 495, 537, 626, 763, 888, 1067
Fnu4HI GCNGC 2 cut(s) 25, 596
FokI GGATG 2 cut(s) 454, 622
FriOI GRGCYC 1 cut(s) 462
Fsp4HI GCNGC 2 cut(s) 25, 596
FspBI CTAG 3 cut(s) 432, 644, 797
GluI GCNGC 2 cut(s) 25, 596
HaeIII GGCC 5 cut(s) 33, 343, 654, 661, 717
HapII CCGG 2 cut(s) 137, 520
Hin1II CATG 9 cut(s) 47, 175, 421, 499, 541, 630, 767, 892, 1071
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 8 cut(s) 47, 54, 86, 131, 613, 802, 902, 1032
HpaII CCGG 2 cut(s) 137, 520
HphI GGTGA 3 cut(s) 70, 359, 1061
Hpy166II GTNNAC 2 cut(s) 423, 850
Hpy188I TCNGA 7 cut(s) 124, 145, 670, 807, 907, 942, 1037
Hpy188III TCNNGA 6 cut(s) 44, 51, 128, 587, 797, 985
Hpy8I GTNNAC 2 cut(s) 423, 850
HpyAV CCTTC 3 cut(s) 350, 429, 728
HpyCH4III ACNGT 2 cut(s) 180, 370
HpyCH4V TGCA 6 cut(s) 203, 229, 240, 267, 598, 1067
HpyF10VI GCNNNNNNNGC 1 cut(s) 30
HpyF3I CTNAG 8 cut(s) 49, 121, 473, 605, 656, 806, 929, 939
Hsp92II CATG 9 cut(s) 47, 175, 421, 499, 541, 630, 767, 892, 1071
Kzo9I GATC 5 cut(s) 93, 124, 385, 753, 934
LmnI GCTCC 2 cut(s) 465, 1027
Lsp1109I GCAGC 2 cut(s) 36, 582
LweI GCATC 3 cut(s) 14, 309, 690
MabI ACCWGGT 1 cut(s) 696
MaeI CTAG 3 cut(s) 432, 644, 797
MaeIII GTNAC 3 cut(s) 404, 600, 919
MalI GATC 5 cut(s) 95, 126, 387, 755, 936
MboI GATC 5 cut(s) 93, 124, 385, 753, 934
MboII GAAGA 5 cut(s) 329, 571, 587, 877, 1064
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 1 cut(s) 934
MhlI GDGCHC 2 cut(s) 462, 837
MluCI AATT 8 cut(s) 252, 503, 723, 843, 873, 909, 977, 988
MlyI GAGTC 5 cut(s) 41, 63, 140, 607, 911
MmeI TCCRAC 2 cut(s) 480, 766
MnlI CCTC 9 cut(s) 46, 290, 468, 559, 600, 633, 665, 672, 948
MroXI GAANNNNTTC 1 cut(s) 992
MseI TTAA 3 cut(s) 702, 726, 1008
MslI CAYNNNNRTG 2 cut(s) 170, 669
MspI CCGG 2 cut(s) 137, 520
MspR9I CCNGG 6 cut(s) 18, 345, 520, 639, 698, 957
MunI CAATTG 1 cut(s) 873
MvaI CCWGG 5 cut(s) 18, 345, 639, 698, 957
MwoI GCNNNNNNNGC 1 cut(s) 30
NciI CCSGG 1 cut(s) 520
NcoI CCATGG 1 cut(s) 763
NdeII GATC 5 cut(s) 93, 124, 385, 753, 934
NlaIII CATG 9 cut(s) 47, 175, 421, 499, 541, 630, 767, 892, 1071
NlaIV GGNNCC 5 cut(s) 34, 151, 524, 746, 769
NmuCI GTSAC 2 cut(s) 600, 919
NspI RCATGY 2 cut(s) 421, 499
PagI TCATGA 1 cut(s) 43
PceI AGGCCT 2 cut(s) 661, 717
PciI ACATGT 2 cut(s) 417, 495
PdmI GAANNNNTTC 1 cut(s) 992
PfeI GAWTC 3 cut(s) 86, 802, 1032
PflMI CCANNNNNTGG 1 cut(s) 956
PkrI GCNGC 2 cut(s) 26, 597
PleI GAGTC 5 cut(s) 41, 62, 139, 607, 910
PpsI GAGTC 5 cut(s) 41, 62, 139, 607, 910
PscI ACATGT 2 cut(s) 417, 495
PsiI TTATAA 1 cut(s) 822
Psp124BI GAGCTC 1 cut(s) 462
Psp6I CCWGG 5 cut(s) 16, 343, 637, 696, 955
PspGI CCWGG 5 cut(s) 16, 343, 637, 696, 955
PspN4I GGNNCC 5 cut(s) 34, 151, 524, 746, 769
PspPI GGNCC 5 cut(s) 32, 150, 184, 522, 914
PstI CTGCAG 1 cut(s) 600
PstNI CAGNNNCTG 1 cut(s) 604
PsuI RGATCY 1 cut(s) 934
RsaI GTAC 2 cut(s) 416, 494
RsaNI GTAC 2 cut(s) 415, 493
RseI CAYNNNNRTG 2 cut(s) 170, 669
SacI GAGCTC 1 cut(s) 462
SaqAI TTAA 3 cut(s) 702, 726, 1008
SatI GCNGC 2 cut(s) 25, 596
Sau3AI GATC 5 cut(s) 93, 124, 385, 753, 934
Sau96I GGNCC 5 cut(s) 32, 150, 184, 522, 914
SchI GAGTC 5 cut(s) 41, 63, 140, 607, 911
ScrFI CCNGG 6 cut(s) 18, 345, 520, 639, 698, 957
SduI GDGCHC 2 cut(s) 462, 837
SetI ASST 9 cut(s) 29, 264, 309, 349, 413, 462, 485, 702, 919
SexAI ACCWGGT 1 cut(s) 696
SfaNI GCATC 3 cut(s) 14, 309, 690
SfcI CTRYAG 1 cut(s) 596
SinI GGWCC 4 cut(s) 150, 184, 522, 914
SmiMI CAYNNNNRTG 2 cut(s) 170, 669
SmlI CTYRAG 1 cut(s) 899
SmoI CTYRAG 1 cut(s) 899
Sse9I AATT 8 cut(s) 252, 503, 723, 843, 873, 909, 977, 988
SseBI AGGCCT 2 cut(s) 661, 717
SsiI CCGC 1 cut(s) 924
SspMI CTAG 3 cut(s) 432, 644, 797
SstI GAGCTC 1 cut(s) 462
StuI AGGCCT 2 cut(s) 661, 717
StyD4I CCNGG 6 cut(s) 16, 343, 518, 637, 696, 955
StyI CCWWGG 4 cut(s) 312, 483, 763, 1023
TaaI ACNGT 2 cut(s) 180, 370
TaqI TCGA 3 cut(s) 96, 396, 984
TasI AATT 8 cut(s) 252, 503, 723, 843, 873, 909, 977, 988
TatI WGTACW 1 cut(s) 414
TfiI GAWTC 3 cut(s) 86, 802, 1032
Tru1I TTAA 3 cut(s) 702, 726, 1008
Tru9I TTAA 3 cut(s) 702, 726, 1008
TscAI CASTG 2 cut(s) 96, 609
TseFI GTSAC 2 cut(s) 600, 919
TseI GCWGC 2 cut(s) 24, 595
Tsp45I GTSAC 2 cut(s) 600, 919
TspDTI ATGAA 4 cut(s) 228, 526, 572, 877
TspRI CASTG 2 cut(s) 96, 609
Van91I CCANNNNNTGG 1 cut(s) 956
VpaK11BI GGWCC 4 cut(s) 150, 184, 522, 914
XapI RAATTY 2 cut(s) 252, 977
XbaI TCTAGA 1 cut(s) 796
XceI RCATGY 2 cut(s) 421, 499
XcmI CCANNNNNNNNNTGG 1 cut(s) 701
XmnI GAANNNNTTC 1 cut(s) 992
XspI CTAG 3 cut(s) 432, 644, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.