pycom10g22500

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001297
Physical Location & Seq
Forward (+)
24865910 .. 24867456
1547 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g22500.3

Sequence Viewer

Length: 1011 bp
ATGGCACTATCGCAGCTCCCAGCAGGCTCCCAAAGCTCTAATTCGCTATTCAGCGTACTAGAGATCGCCAACGAGCCCTTAATCTCAGTCCCACAATGTTACGTGCGTTTGGAACAAGAGCCAACTCCATCTGAACTCTCTGATAGTCATAAGGGCACCTTCCCTTCTATCCCTACTATCGATATGACGAAATTTGTTAACGGAGAAACCTCGGACTTTGCAAGTCAACTAGAAAAACTGCACTCAACTTGCAAAGATTGGGGCATTTTTCAGTTGGTGAACCATGGAGTTAGCCCTATGCTGTTAGAGAAGCTGAAACATGAGGTTCAAGTGTTCTTTAAGCTTCCCTTGGAAGAGAAAATGAAATATACAATAAGATCAGGTGATTTTGAAGGGTATGGAAACATAGTCAAGACCAAAGACCAAAAGCTTGACTGGGGTGATAGGTTCTATATGATAACCAACCCTATTCATCGAAGAAATCCGTACCTCTTCGCGGAGCTCCCTTCTTCCCTCAGGAATACCTTGGAGTCATACTTTATGGAATTGGATCAACTTGCCATGAGACTTCTTGGGTTTATGGCGAAGGCTCTGAAGATAGAGATGAGTGAGATAGAGGAGTTGTTTGATAAAGATGGGATGCAATCAGTGAGGATGACATACTATCCTCCATGTCCACAACCAGAGCTGGTTGCAGGGCTCACACCTCACTCAGATGCAACTGGGATCACCATCCTCAACCAGCTTAATGGAGACGGTCTCCAAATTAAAAAAGACGGGGTTTGGATGCCTGTCAACTTCCATAAAGATGCTTTTGTGGTGAATGTAGGGGACATCTTTGAGGTTCGTCTTATATATTCGATTAATCCTATACTCATCACACTTTTCACATTACATTTGTACCACATCTCTAATAGAGGATATGTTAAAAGTAGCATTTTTGTATATATTCTTCTTTCCTCACCCTTCATTTCTGCCCTATATATTCAACCACAAAGTAGTAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

337

Amino Acids

38.21

Weight (kDa)

5.87

Isoelectric Point (pI)

51.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 155
AccII CGCG 1 cut(s) 497
AciI CCGC 1 cut(s) 497
AclWI GGATC 2 cut(s) 558, 734
AcsI RAATTY 1 cut(s) 191
AcuI CTGAAG 1 cut(s) 614
AfaI GTAC 3 cut(s) 57, 488, 902
AfiI CCNNNNNNNGG 1 cut(s) 496
AgsI TTSAA 3 cut(s) 329, 392, 989
AluBI AGCT 8 cut(s) 16, 36, 313, 343, 430, 502, 688, 745
AluI AGCT 8 cut(s) 16, 36, 313, 343, 430, 502, 688, 745
Alw21I GWGCWC 1 cut(s) 504
Alw26I GTCTC 3 cut(s) 559, 747, 764
AlwI GGATC 2 cut(s) 558, 734
ApeKI GCWGC 1 cut(s) 13
ApoI RAATTY 1 cut(s) 191
AseI ATTAAT 1 cut(s) 864
AsuHPI GGTGA 6 cut(s) 289, 395, 452, 721, 832, 954
AxyI CCTNAGG 1 cut(s) 515
BaeGI GKGCMC 1 cut(s) 158
BaeI ACNNNNGTAYC 2 cut(s) 884, 917
BanI GGYRCC 1 cut(s) 155
BanII GRGCYC 3 cut(s) 78, 504, 702
BarI GAAGNNNNNNTAC 2 cut(s) 936, 968
Bbv12I GWGCWC 1 cut(s) 504
BbvI GCAGC 1 cut(s) 25
BccI CCATC 3 cut(s) 136, 629, 740
BcoDI GTCTC 3 cut(s) 559, 747, 764
BfaI CTAG 2 cut(s) 59, 230
BisI GCNGC 1 cut(s) 14
BlsI GCNGC 1 cut(s) 15
BmiI GGNNCC 2 cut(s) 28, 157
BmrI ACTGGG 2 cut(s) 445, 732
BmsI GCATC 4 cut(s) 630, 706, 777, 799
BmuI ACTGGG 2 cut(s) 445, 732
BplI GAGNNNNNCTC 2 cut(s) 744, 776
Bsa29I ATCGAT 1 cut(s) 180
BsaAI YACGTR 1 cut(s) 103
BsaBI GATNNNNATC 1 cut(s) 731
BsaI GGTCTC 1 cut(s) 764
BsaJI CCNNGG 4 cut(s) 210, 283, 348, 525
Bsc4I CCNNNNNNNGG 1 cut(s) 496
Bse1I ACTGG 2 cut(s) 440, 727
Bse21I CCTNAGG 1 cut(s) 515
Bse8I GATNNNNATC 1 cut(s) 731
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 4 cut(s) 210, 283, 348, 525
BseGI GGATG 4 cut(s) 645, 660, 732, 792
BseJI GATNNNNATC 1 cut(s) 731
BseLI CCNNNNNNNGG 1 cut(s) 496
BseMII CTCAG 3 cut(s) 99, 529, 726
BseNI ACTGG 2 cut(s) 440, 727
BseRI GAGGAG 1 cut(s) 632
BseSI GKGCMC 1 cut(s) 158
BseXI GCAGC 1 cut(s) 25
BseYI CCCAGC 1 cut(s) 19
BsgI GTGCAG 1 cut(s) 224
Bsh1236I CGCG 1 cut(s) 497
BshNI GGYRCC 1 cut(s) 155
BshVI ATCGAT 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 504
BslFI GGGAC 2 cut(s) 74, 845
BslI CCNNNNNNNGG 1 cut(s) 496
BsmAI GTCTC 3 cut(s) 559, 747, 764
BsmBI CGTCTC 1 cut(s) 747
BsmFI GGGAC 2 cut(s) 74, 845
Bso31I GGTCTC 1 cut(s) 764
Bsp1286I GDGCHC 4 cut(s) 78, 158, 504, 702
Bsp143I GATC 4 cut(s) 63, 377, 550, 726
Bsp19I CCATGG 1 cut(s) 283
BspACI CCGC 1 cut(s) 497
BspCNI CTCAG 3 cut(s) 98, 528, 725
BspDI ATCGAT 1 cut(s) 180
BspFNI CGCG 1 cut(s) 497
BspLI GGNNCC 2 cut(s) 28, 157
BspPI GGATC 2 cut(s) 558, 734
BspT107I GGYRCC 1 cut(s) 155
BspTNI GGTCTC 1 cut(s) 764
BsrI ACTGG 2 cut(s) 440, 727
BssECI CCNNGG 4 cut(s) 210, 283, 348, 525
BssMI GATC 4 cut(s) 63, 377, 550, 726
BssT1I CCWWGG 3 cut(s) 283, 348, 525
Bst4CI ACNGT 1 cut(s) 758
Bst6I CTCTTC 2 cut(s) 348, 497
BstBAI YACGTR 1 cut(s) 103
BstC8I GCNNGC 1 cut(s) 25
BstDEI CTNAG 3 cut(s) 85, 515, 712
BstDSI CCRYGG 1 cut(s) 283
BstF5I GGATG 4 cut(s) 645, 660, 732, 792
BstFNI CGCG 1 cut(s) 497
BstKTI GATC 4 cut(s) 66, 380, 553, 729
BstMAI GTCTC 3 cut(s) 559, 747, 764
BstMBI GATC 4 cut(s) 63, 377, 550, 726
BstMWI GCNNNNNNNGC 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 158
BstUI CGCG 1 cut(s) 497
BstV1I GCAGC 1 cut(s) 25
BstXI CCANNNNNNTGG 1 cut(s) 749
Bsu15I ATCGAT 1 cut(s) 180
Bsu36I CCTNAGG 1 cut(s) 515
BsuTUI ATCGAT 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 283
BtsCI GGATG 4 cut(s) 645, 660, 732, 792
BtsIMutI CAGTG 1 cut(s) 654
Cac8I GCNNGC 1 cut(s) 25
ClaI ATCGAT 1 cut(s) 180
Csp6I GTAC 3 cut(s) 56, 487, 901
CviAII CATG 4 cut(s) 284, 320, 562, 672
CviQI GTAC 3 cut(s) 56, 487, 901
DdeI CTNAG 3 cut(s) 85, 515, 712
DpnI GATC 4 cut(s) 65, 379, 552, 728
DpnII GATC 4 cut(s) 63, 377, 550, 726
Eam1104I CTCTTC 2 cut(s) 348, 497
EarI CTCTTC 2 cut(s) 348, 497
Ecl136II GAGCTC 1 cut(s) 502
Eco130I CCWWGG 3 cut(s) 283, 348, 525
Eco24I GRGCYC 3 cut(s) 78, 504, 702
Eco31I GGTCTC 1 cut(s) 764
Eco53kI GAGCTC 1 cut(s) 502
Eco57I CTGAAG 1 cut(s) 614
Eco81I CCTNAGG 1 cut(s) 515
EcoICRI GAGCTC 1 cut(s) 502
EcoT14I CCWWGG 3 cut(s) 283, 348, 525
EcoT38I GRGCYC 3 cut(s) 78, 504, 702
ErhI CCWWGG 3 cut(s) 283, 348, 525
Esp3I CGTCTC 1 cut(s) 747
FaeI CATG 4 cut(s) 287, 323, 565, 675
FalI AAGNNNNNCTT 4 cut(s) 143, 175, 332, 364
FaqI GGGAC 2 cut(s) 74, 845
FatI CATG 4 cut(s) 283, 319, 561, 671
Fnu4HI GCNGC 1 cut(s) 14
FokI GGATG 4 cut(s) 652, 667, 719, 799
FriOI GRGCYC 3 cut(s) 78, 504, 702
Fsp4HI GCNGC 1 cut(s) 14
FspBI CTAG 2 cut(s) 59, 230
GluI GCNGC 1 cut(s) 14
GsaI CCCAGC 1 cut(s) 23
Hin1II CATG 4 cut(s) 287, 323, 565, 675
HincII GTYRAC 3 cut(s) 199, 227, 796
HindII GTYRAC 3 cut(s) 199, 227, 796
HindIII AAGCTT 2 cut(s) 341, 428
HinfI GANTC 1 cut(s) 530
HpaI GTTAAC 1 cut(s) 199
HphI GGTGA 6 cut(s) 289, 395, 452, 721, 832, 954
Hpy166II GTNNAC 5 cut(s) 199, 227, 280, 677, 796
Hpy188I TCNGA 5 cut(s) 133, 142, 214, 594, 715
Hpy188III TCNNGA 2 cut(s) 412, 517
Hpy8I GTNNAC 5 cut(s) 199, 227, 280, 677, 796
HpyAV CCTTC 6 cut(s) 169, 174, 386, 516, 580, 976
HpyCH4III ACNGT 1 cut(s) 758
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 6 cut(s) 221, 241, 252, 643, 695, 719
HpyF10VI GCNNNNNNNGC 1 cut(s) 33
HpyF3I CTNAG 3 cut(s) 85, 515, 712
HpySE526I ACGT 1 cut(s) 102
Hsp92II CATG 4 cut(s) 287, 323, 565, 675
KspAI GTTAAC 1 cut(s) 199
Kzo9I GATC 4 cut(s) 63, 377, 550, 726
LmnI GCTCC 4 cut(s) 21, 32, 499, 507
Lsp1109I GCAGC 1 cut(s) 25
LweI GCATC 4 cut(s) 630, 706, 777, 799
MaeI CTAG 2 cut(s) 59, 230
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 1 cut(s) 98
MalI GATC 4 cut(s) 65, 379, 552, 728
MboI GATC 4 cut(s) 63, 377, 550, 726
MboII GAAGA 6 cut(s) 365, 484, 489, 501, 607, 944
MhlI GDGCHC 4 cut(s) 78, 158, 504, 702
MluCI AATT 4 cut(s) 40, 191, 545, 765
MlyI GAGTC 1 cut(s) 539
MseI TTAA 7 cut(s) 80, 198, 339, 747, 768, 864, 927
MslI CAYNNNNRTG 2 cut(s) 714, 807
MvnI CGCG 1 cut(s) 497
MwoI GCNNNNNNNGC 1 cut(s) 33
NcoI CCATGG 1 cut(s) 283
NdeII GATC 4 cut(s) 63, 377, 550, 726
NlaIII CATG 4 cut(s) 287, 323, 565, 675
NlaIV GGNNCC 2 cut(s) 28, 157
PkrI GCNGC 1 cut(s) 15
PleI GAGTC 1 cut(s) 538
PpsI GAGTC 1 cut(s) 538
Ppu21I YACGTR 1 cut(s) 103
PshBI ATTAAT 1 cut(s) 864
Psp124BI GAGCTC 1 cut(s) 504
PspFI CCCAGC 1 cut(s) 19
PspN4I GGNNCC 2 cut(s) 28, 157
RsaI GTAC 3 cut(s) 57, 488, 902
RsaNI GTAC 3 cut(s) 56, 487, 901
RseI CAYNNNNRTG 2 cut(s) 714, 807
SacI GAGCTC 1 cut(s) 504
SaqAI TTAA 7 cut(s) 80, 198, 339, 747, 768, 864, 927
SatI GCNGC 1 cut(s) 14
Sau3AI GATC 4 cut(s) 63, 377, 550, 726
SchI GAGTC 1 cut(s) 539
SduI GDGCHC 4 cut(s) 78, 158, 504, 702
SfaNI GCATC 4 cut(s) 630, 706, 777, 799
SmiMI CAYNNNNRTG 2 cut(s) 714, 807
Sse9I AATT 4 cut(s) 40, 191, 545, 765
SsiI CCGC 1 cut(s) 497
SspMI CTAG 2 cut(s) 59, 230
SstI GAGCTC 1 cut(s) 504
StyI CCWWGG 3 cut(s) 283, 348, 525
TaaI ACNGT 1 cut(s) 758
TaiI ACGT 1 cut(s) 105
TaqI TCGA 3 cut(s) 180, 475, 860
TasI AATT 4 cut(s) 40, 191, 545, 765
Tru1I TTAA 7 cut(s) 80, 198, 339, 747, 768, 864, 927
Tru9I TTAA 7 cut(s) 80, 198, 339, 747, 768, 864, 927
TscAI CASTG 1 cut(s) 654
TseI GCWGC 1 cut(s) 13
TspDTI ATGAA 3 cut(s) 377, 461, 958
TspGWI ACGGA 2 cut(s) 216, 474
TspRI CASTG 1 cut(s) 654
VspI ATTAAT 1 cut(s) 864
XapI RAATTY 1 cut(s) 191
XspI CTAG 2 cut(s) 59, 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.