MD05G1292600.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001297
Physical Location & Seq
Forward (+)
42403159 .. 42406601
3443 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1292600.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGGCATCACTATCGCAGCTCCCAGCAGGCTCTGAAAGCTCTAATTCGCTATTCAGCGTCATAGAGACCGCCAAAGAGCCCTTAACTTCAGTCCCACAATGTTACGTTCGTTTGGAACAAGAACCAACTCCATCTGAACTCTCTGATGATAATAAGGGCACCTTCCGTTCTATCCCTACCATCGACATGACGAAATTAGTTAAGGGAGAAACCTCAGACTTTGCAAATCAACTGGAGAAACTGCACTCAGCTTGCAAAGACTGGGGCATTTTTCAGTTGGTGAACCATGGAGTTAACCCTATGCTGTTAGAGAATCTGGAACATGAGATTGAAGTATTCTTTAAGCTTCCCTTGGAAGAGAAAATGAAATATGCAATAAGGTCAGGTGATGTTGAAGGCTATGGAAACATAGCCAAGACCAAAGACCAAAAGCTTGACTGGGGTGATAGGTTCTATATAATAACCAACCCTATTCATCGAAGAAAGCCGTACCTCTTCGCAGAGCTCCCTTCATCCATCAGGAATACCTTGGAGTCATACATTATGGAATTGGACCAACTTGCCATGAGACTTCTTGGGTTTATGGTGAAAGCTCTGAAGACAGATATGAGAGAGATAGAGGAGTTGTTCGATACAGACGGGATGCAATCAGTGAGGATGACATACTATCCTCCATGTCCACAACCAGAGCTGGTTGTAGGGATCTCACCTCACTCAGATGCATCTGGGATCACCATCCTCAACCAGCTTAATGGAGTTGACGGTCTCCAAATTAAAAAAGACGGAGCTTGGATGCCCGTCAACTTCCAAAAAGATGCCTTTGTGGTGAATGTCGGGGACATCTTTGAGATGCTGAGCAACGGAGTTTACAAAAGCATTGAACACAGGGCAATGGTGAGTTCAGAGAAAGAAAGGGTATCGGTTGGGATGTTCTTCAACCCTAAATTTGAGGCAGAGATTGGGCCACTCAGAAGCTTGATAACCCCAGAAAACCCACCACTGTTCCAAAGGGTTGGGATGGAAAAGTACGTCAACGATTTCTTCTCCCGCCGTAGGCTTGACGGGAAATCGTACTTGGAGCAAATGAAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

365

Amino Acids

41.4

Weight (kDa)

5.29

Isoelectric Point (pI)

43.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 58 - 157 3.1e-27 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 216 - 314 1.5e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 158
AciI CCGC 2 cut(s) 69, 1048
AclWI GGATC 2 cut(s) 710, 737
AcsI RAATTY 1 cut(s) 944
AcuI CTGAAG 2 cut(s) 72, 617
AfaI GTAC 3 cut(s) 491, 1028, 1073
AfiI CCNNNNNNNGG 1 cut(s) 1053
AgsI TTSAA 4 cut(s) 332, 395, 881, 937
AjuI GAANNNNNNNTTGG 2 cut(s) 1058, 1090
Alw21I GWGCWC 1 cut(s) 507
Alw26I GTCTC 3 cut(s) 59, 562, 770
AlwI GGATC 2 cut(s) 710, 737
AlwNI CAGNNNCTG 1 cut(s) 32
AoxI GGCC 1 cut(s) 962
ApeKI GCWGC 1 cut(s) 16
ApoI RAATTY 1 cut(s) 944
AspS9I GGNCC 2 cut(s) 553, 962
AsuHPI GGTGA 8 cut(s) 292, 398, 455, 598, 699, 724, 838, 907
AvaII GGWCC 1 cut(s) 553
BaeGI GKGCMC 1 cut(s) 161
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 2 cut(s) 81, 507
BbsI GAAGAC 1 cut(s) 605
Bbv12I GWGCWC 1 cut(s) 507
BbvI GCAGC 1 cut(s) 28
BccI CCATC 5 cut(s) 139, 188, 524, 743, 1012
BceAI ACGGC 2 cut(s) 472, 1035
BcgI CGANNNNNNTGC 1 cut(s) 28
BcoDI GTCTC 3 cut(s) 59, 562, 770
BisI GCNGC 1 cut(s) 17
BlpI GCTNAGC 1 cut(s) 854
BlsI GCNGC 1 cut(s) 18
Bme18I GGWCC 1 cut(s) 553
BmgT120I GGNCC 2 cut(s) 553, 962
BmiI GGNNCC 1 cut(s) 160
BmrI ACTGGG 2 cut(s) 271, 448
BmsI GCATC 7 cut(s) 14, 633, 709, 731, 783, 805, 840
BmuI ACTGGG 2 cut(s) 271, 448
BpiI GAAGAC 1 cut(s) 605
BpmI CTGGAG 1 cut(s) 254
Bpu1102I GCTNAGC 1 cut(s) 854
BsaBI GATNNNNATC 1 cut(s) 734
BsaI GGTCTC 2 cut(s) 59, 770
BsaJI CCNNGG 3 cut(s) 286, 351, 528
Bsc4I CCNNNNNNNGG 1 cut(s) 1053
Bse1I ACTGG 3 cut(s) 237, 266, 443
Bse3DI GCAATG 1 cut(s) 897
Bse8I GATNNNNATC 1 cut(s) 734
BseDI CCNNGG 3 cut(s) 286, 351, 528
BseGI GGATG 7 cut(s) 512, 648, 663, 735, 798, 933, 1023
BseJI GATNNNNATC 1 cut(s) 734
BseLI CCNNNNNNNGG 1 cut(s) 1053
BseMI GCAATG 1 cut(s) 897
BseMII CTCAG 5 cut(s) 228, 261, 729, 845, 982
BseNI ACTGG 3 cut(s) 237, 266, 443
BseRI GAGGAG 1 cut(s) 635
BseSI GKGCMC 1 cut(s) 161
BseXI GCAGC 1 cut(s) 28
BseYI CCCAGC 1 cut(s) 22
BsgI GTGCAG 1 cut(s) 227
BshFI GGCC 1 cut(s) 964
BshNI GGYRCC 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 507
BslFI GGGAC 2 cut(s) 77, 851
BslI CCNNNNNNNGG 1 cut(s) 1053
BsmAI GTCTC 3 cut(s) 59, 562, 770
BsmFI GGGAC 2 cut(s) 77, 851
BsnI GGCC 1 cut(s) 964
Bso31I GGTCTC 2 cut(s) 59, 770
Bsp1286I GDGCHC 3 cut(s) 81, 161, 507
Bsp143I GATC 2 cut(s) 702, 729
Bsp1720I GCTNAGC 1 cut(s) 854
Bsp19I CCATGG 1 cut(s) 286
BspACI CCGC 2 cut(s) 69, 1048
BspANI GGCC 1 cut(s) 964
BspCNI CTCAG 5 cut(s) 227, 260, 728, 846, 981
BspLI GGNNCC 1 cut(s) 160
BspPI GGATC 2 cut(s) 710, 737
BspT107I GGYRCC 1 cut(s) 158
BspTNI GGTCTC 2 cut(s) 59, 770
BsrDI GCAATG 1 cut(s) 897
BsrI ACTGG 3 cut(s) 237, 266, 443
BssECI CCNNGG 3 cut(s) 286, 351, 528
BssMI GATC 2 cut(s) 702, 729
BssT1I CCWWGG 3 cut(s) 286, 351, 528
Bst4CI ACNGT 2 cut(s) 764, 1002
Bst6I CTCTTC 2 cut(s) 351, 500
BstC8I GCNNGC 2 cut(s) 28, 253
BstDEI CTNAG 5 cut(s) 214, 247, 715, 854, 968
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 7 cut(s) 512, 648, 663, 735, 798, 933, 1023
BstKTI GATC 2 cut(s) 705, 732
BstMAI GTCTC 3 cut(s) 59, 562, 770
BstMBI GATC 2 cut(s) 702, 729
BstMWI GCNNNNNNNGC 1 cut(s) 36
BstSLI GKGCMC 1 cut(s) 161
BstV1I GCAGC 1 cut(s) 28
BstV2I GAAGAC 1 cut(s) 605
BstX2I RGATCY 1 cut(s) 702
BstXI CCANNNNNNTGG 2 cut(s) 752, 1013
BstYI RGATCY 1 cut(s) 702
BsuRI GGCC 1 cut(s) 964
BtgI CCRYGG 1 cut(s) 286
BtsCI GGATG 7 cut(s) 512, 648, 663, 735, 798, 933, 1023
BtsIMutI CAGTG 2 cut(s) 657, 998
Cac8I GCNNGC 2 cut(s) 28, 253
CaiI CAGNNNCTG 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 553, 962
CseI GACGC 1 cut(s) 46
Csp6I GTAC 3 cut(s) 490, 1027, 1072
CviAII CATG 5 cut(s) 187, 287, 323, 565, 675
CviQI GTAC 3 cut(s) 490, 1027, 1072
DdeI CTNAG 5 cut(s) 214, 247, 715, 854, 968
DpnI GATC 2 cut(s) 704, 731
DpnII GATC 2 cut(s) 702, 729
Eam1104I CTCTTC 2 cut(s) 351, 500
EarI CTCTTC 2 cut(s) 351, 500
Ecl136II GAGCTC 1 cut(s) 505
Eco130I CCWWGG 3 cut(s) 286, 351, 528
Eco24I GRGCYC 2 cut(s) 81, 507
Eco31I GGTCTC 2 cut(s) 59, 770
Eco47I GGWCC 1 cut(s) 553
Eco53kI GAGCTC 1 cut(s) 505
Eco57I CTGAAG 2 cut(s) 72, 617
EcoICRI GAGCTC 1 cut(s) 505
EcoT14I CCWWGG 3 cut(s) 286, 351, 528
EcoT22I ATGCAT 1 cut(s) 724
EcoT38I GRGCYC 2 cut(s) 81, 507
ErhI CCWWGG 3 cut(s) 286, 351, 528
FaeI CATG 5 cut(s) 190, 290, 326, 568, 678
FalI AAGNNNNNCTT 4 cut(s) 146, 178, 335, 367
FaqI GGGAC 2 cut(s) 77, 851
FatI CATG 5 cut(s) 186, 286, 322, 564, 674
FauI CCCGC 1 cut(s) 1055
Fnu4HI GCNGC 1 cut(s) 17
FokI GGATG 7 cut(s) 499, 655, 670, 722, 805, 940, 1030
FriOI GRGCYC 2 cut(s) 81, 507
Fsp4HI GCNGC 1 cut(s) 17
GluI GCNGC 1 cut(s) 17
GsaI CCCAGC 1 cut(s) 26
GsuI CTGGAG 1 cut(s) 254
HaeIII GGCC 1 cut(s) 964
HgaI GACGC 1 cut(s) 46
Hin1II CATG 5 cut(s) 190, 290, 326, 568, 678
HincII GTYRAC 4 cut(s) 295, 760, 802, 1033
HindII GTYRAC 4 cut(s) 295, 760, 802, 1033
HindIII AAGCTT 3 cut(s) 344, 431, 973
HinfI GANTC 2 cut(s) 313, 533
HpaI GTTAAC 1 cut(s) 295
HphI GGTGA 8 cut(s) 292, 398, 455, 598, 699, 724, 838, 907
Hpy166II GTNNAC 7 cut(s) 283, 295, 680, 760, 802, 868, 1033
Hpy188I TCNGA 8 cut(s) 34, 136, 145, 217, 597, 718, 904, 971
Hpy188III TCNNGA 2 cut(s) 317, 520
Hpy8I GTNNAC 7 cut(s) 283, 295, 680, 760, 802, 868, 1033
HpyAV CCTTC 3 cut(s) 172, 389, 519
HpyCH4III ACNGT 2 cut(s) 764, 1002
HpyCH4IV ACGT 2 cut(s) 105, 1029
HpyCH4V TGCA 6 cut(s) 224, 244, 255, 374, 646, 722
HpyF10VI GCNNNNNNNGC 1 cut(s) 36
HpyF3I CTNAG 5 cut(s) 214, 247, 715, 854, 968
HpySE526I ACGT 2 cut(s) 105, 1029
Hsp92II CATG 5 cut(s) 190, 290, 326, 568, 678
KspAI GTTAAC 1 cut(s) 295
Kzo9I GATC 2 cut(s) 702, 729
LmnI GCTCC 4 cut(s) 24, 510, 785, 1078
Lsp1109I GCAGC 1 cut(s) 28
LweI GCATC 7 cut(s) 14, 633, 709, 731, 783, 805, 840
MaeII ACGT 2 cut(s) 105, 1029
MaeIII GTNAC 1 cut(s) 101
MalI GATC 2 cut(s) 704, 731
MboI GATC 2 cut(s) 702, 729
MboII GAAGA 6 cut(s) 368, 487, 492, 610, 925, 1033
MflI RGATCY 1 cut(s) 702
MhlI GDGCHC 3 cut(s) 81, 161, 507
MluCI AATT 5 cut(s) 43, 194, 548, 771, 944
MlyI GAGTC 1 cut(s) 542
MnlI CCTC 8 cut(s) 223, 503, 613, 648, 681, 720, 749, 943
Mph1103I ATGCAT 1 cut(s) 724
MseI TTAA 6 cut(s) 83, 201, 294, 342, 750, 774
MslI CAYNNNNRTG 2 cut(s) 185, 717
MwoI GCNNNNNNNGC 1 cut(s) 36
NcoI CCATGG 1 cut(s) 286
NdeII GATC 2 cut(s) 702, 729
NlaIII CATG 5 cut(s) 190, 290, 326, 568, 678
NlaIV GGNNCC 1 cut(s) 160
NsiI ATGCAT 1 cut(s) 724
PfeI GAWTC 1 cut(s) 313
PkrI GCNGC 1 cut(s) 18
PleI GAGTC 1 cut(s) 541
PpsI GAGTC 1 cut(s) 541
Psp124BI GAGCTC 1 cut(s) 507
PspFI CCCAGC 1 cut(s) 22
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 2 cut(s) 553, 962
PstNI CAGNNNCTG 1 cut(s) 32
PsuI RGATCY 1 cut(s) 702
RsaI GTAC 3 cut(s) 491, 1028, 1073
RsaNI GTAC 3 cut(s) 490, 1027, 1072
RseI CAYNNNNRTG 2 cut(s) 185, 717
SacI GAGCTC 1 cut(s) 507
SaqAI TTAA 6 cut(s) 83, 201, 294, 342, 750, 774
SatI GCNGC 1 cut(s) 17
Sau3AI GATC 2 cut(s) 702, 729
Sau96I GGNCC 2 cut(s) 553, 962
SchI GAGTC 1 cut(s) 542
SduI GDGCHC 3 cut(s) 81, 161, 507
SfaNI GCATC 7 cut(s) 14, 633, 709, 731, 783, 805, 840
SinI GGWCC 1 cut(s) 553
SmiMI CAYNNNNRTG 2 cut(s) 185, 717
Sse9I AATT 5 cut(s) 43, 194, 548, 771, 944
SsiI CCGC 2 cut(s) 69, 1048
SstI GAGCTC 1 cut(s) 507
StyI CCWWGG 3 cut(s) 286, 351, 528
TaaI ACNGT 2 cut(s) 764, 1002
TaiI ACGT 2 cut(s) 108, 1032
TaqI TCGA 3 cut(s) 183, 478, 630
TasI AATT 5 cut(s) 43, 194, 548, 771, 944
TfiI GAWTC 1 cut(s) 313
Tru1I TTAA 6 cut(s) 83, 201, 294, 342, 750, 774
Tru9I TTAA 6 cut(s) 83, 201, 294, 342, 750, 774
TscAI CASTG 2 cut(s) 657, 1005
TseI GCWGC 1 cut(s) 16
TspDTI ATGAA 3 cut(s) 380, 464, 501
TspGWI ACGGA 3 cut(s) 155, 798, 876
TspRI CASTG 2 cut(s) 657, 1005
VpaK11BI GGWCC 1 cut(s) 553
XapI RAATTY 1 cut(s) 944
Zsp2I ATGCAT 1 cut(s) 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.