Rorug02G0302600

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001297
Physical Location & Seq
Forward (+)
33778264 .. 33781655
3392 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0302600.1

Sequence Viewer

Length: 2661 bp
ATGATGGCTTTCCTGAGGGTTGCTGATGCATATGAAGAGAGTGATGCGGAGCTCAAAGTATGGGTTAAACAACTAAGAGACATTGCTCACGAAAGTGAAGATGTTCTTGATGAATTTACCCTTCTGCAAGCACATGATCATGGGGAGGGATTATATGGTTCAATCCATAAGCTTTCTTGCTGCATCAAGAATACAAAAGCTCGATATCGAATTGCTTCGGAGTTACAAGCCATAAACTCCAGGATCAAAAAAATCTCTGAGGTTCATAAGCGACTCCGCCACAAATTCAATATGGCTGAACAAGGCTCAAGCTCCAAAAGTGCAGGTTACCTGTTGGAGGACCATCGAGGTGATGCTTTACTTCTAGAGAAAATTGATATAGTGGGCATAGACGAGCCTATTAAGCAGATGGTTGGGAGGCTGGTCAATGGTGGTTGTGGACGTGAGGTAGTTTCTGTGGCAGGAATGGGAGGAATGGGGAAAACTACCCTGGCGAAGCAAGTCTATGATGCAGCAGAAGTGAAGAAACATTTCAAATTACGTGCTTGGATCACTTTTACTCAGTCTTTCAGGCTAGCGGAACTTCTCAAAGACATCGTTCAACAGCTCCACCAAGCAATCCGAAGTCCAGTTCCACAAGAAATCAATAGCATGAATACCAACCAGCTGAAAACAATAATTAAAGACTTCCTACAGAAGAGGAGGTATCTCATTGTCTTGGATGATGTGTGGCACTTATATGGATGGGATGCCTTGAAGTATGCACTGCCTAATTATATTTACGGCAGCAGAGTGATACTAACTACTCGAAACGCAGATATAGCCTCCACAACAAGCACGGAATCTGGAGGTGAAGTCTATACTTTGAAGCCCTTGCCCCTAGTGGAGTCATGGGAGCTCCTGTGCAAGAAGACATTCCAAGGGAATTCCTGCCCTCCTCATTTAGAGAAAATCTGTAACTATGTTCTGAGAAAGTGTGAGGGGCTACCCCTTGCTATTGTGGCAATTAGTGGAGTTCTGGCTGCTAAAGATAAGCGCAGAATTGATGAGTGGGAAATGGTTGGCCACAGTCTCGGGGCTGAAATTGAGGAGGGCTTCATTGAAGAAAAACAAGGAAAGACATTGGAAGAGGTTGCAGAGGATTACCTCAATGAACTATTGAATAGGAGCATGATTCAAGCAGCAGAGACAACAACTGATGGAAGGGTCACAAAATTTCGTATCCATGATCTCTTCCGCGAAATTATCACCTCCAGGACAAGAAGTCAGAACTTTGCCACAAAAGCCAAAGAGCAGAATGTAACATTGCCAGAAAAAATCCGTCGCCTGTCAATACATAACACACTGCAATATGTTCAGACAAACAGGTGTGCTTCTCAACTACGTTCTTTTTTTATGTTCACAGTTGCTGAGAAGCCATCACTACAAAGACTATTCCCAGCCGGCTTTGTGCTGTTTAATGTGTTAGATTTGCAAAGTACACCTTTAAATGTTTTCCCAGTTGAAGTTGTGAATCTTTACTTTTTAAAGTATCTGAGCTTGAGAGATACCAGGGTAAAAACCATTCCAAGATTTATAGGGAAGCTTCAGAACCTGGAAACTTTGGATCTTAAGCATTCTCAAGTTACAGTTGAAATTCTGAAACTCCAACATCTGCGCTATCTCTTGGTATATCATTATGAGTTTGTTCCTTACGAAGATTTTCACTCAACATATGGTTTTAAGGTCCTTGCAAAAATAGGAGCTTTGAAATCCCTTCAAAAGCTATGTTTCATTGAGGTGAACCAAGGTGGCAGCGCATTATTGAGAGAGCTGGGGAAACTAGTTCAGTTGAGAAGGTTAGGCATTGTTAAGATGAGGAAAAAAGATGGCAAGGCTCTTTCTTCATCCATTGAAAAGTTGTCCAAACTTTATGCATTGTCCATAACTTCAGTGGAAGAAGACGAGATCATTGATTTGTTAAGGGATGATCCACTTGCATTCCTTCAGTATTTGCCCAATCTAGTACATCTTGAGTTGTGTCAGGTTTTTGAAGGAGACACACTATGTTTTGGAGCCGGCGGATTTAAGAAGTTCAAGCATTTATGCCTCGATAAATCTGATGAACTTCGATGCATAGAGATGGAGGTGGGAACAATGCTTTGTATTGAAAATCTAATCATCCGGCGCTGTAAATCACTGGAGAAAGTCCCATCAGGCATTGAACATCTCATCAAACTAAAGTTGCTTAAGTTTTTTTATATGCCAGAAAAACTAATCAAGACTCTACTTCCACATGAGCATGGCAATGATTATTGGAAAGTTGCGCATGTCCCAGAAGTTTATGTCACCTACTTGAGAGAGTTTGGGTGGGAAGTATTCTCATTAGAGGGCTTGAGTGAGACACAGATCTCTTCTCAACCCAGTTCTGTCATGAAGAGCATTGGAACCGGTTGGAACCCTAGCAGCCAGAGAGGGATCTACGCATCGATCGAGTTGTCGTCCGACGGCGGACAACGGAAGCACGGGCTCTACCTCGTTGTTGTTGTGTTGTTGTCATACGGGATCGATTGGCTTAGAGGAACAGCGGTGGTCGACATCAATATCTGGTTCAAAGCTCCTAGGAGGCAGGGGCGGTCATGGGTATCAATGCAAGATACGGTGACTGGCGGCGTGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

886

Amino Acids

100.95

Weight (kDa)

8.72

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 1 - 47 1.7e-10 Rx N-terminal domain
NB-ARC PF00931 132 - 310 1.2e-40 NB-ARC domain
AAA_16 PF13191 137 - 243 1.7e-06 AAA ATPase domain
WHD_DRP PF23559 363 - 416 1e-14 Disease resistance protein Winged helix domain
LRR_14 PF23598 460 - 645 3.4e-23 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2307
Acc36I ACCTGC 1 cut(s) 314
AccI GTMKAC 1 cut(s) 2574
AccII CGCG 1 cut(s) 1239
AciI CCGC 9 cut(s) 47, 277, 578, 1237, 2061, 2490, 2567, 2614, 2649
AclWI GGATC 6 cut(s) 251, 557, 1614, 1964, 2465, 2552
AcoI YGGCCR 1 cut(s) 1063
AcsI RAATTY 5 cut(s) 113, 284, 925, 1214, 1635
AcuI CTGAAG 3 cut(s) 1571, 1914, 1970
AfaI GTAC 2 cut(s) 1480, 2007
AfiI CCNNNNNNNGG 1 cut(s) 337
AflII CTTAAG 2 cut(s) 1610, 2228
AgeI ACCGGT 1 cut(s) 2429
AhdI GACNNNNNGTC 1 cut(s) 1263
AhlI ACTAGT 1 cut(s) 1822
AjiI CACGTC 1 cut(s) 443
AjnI CCWGG 5 cut(s) 239, 489, 1253, 1550, 1593
AleI CACNNNNGTG 1 cut(s) 93
Alw21I GWGCWC 2 cut(s) 54, 900
Alw26I GTCTC 5 cut(s) 72, 1076, 1181, 2031, 2375
AlwI GGATC 6 cut(s) 251, 557, 1614, 1964, 2465, 2552
AlwNI CAGNNNCTG 2 cut(s) 1409, 1594
Ama87I CYCGRG 1 cut(s) 1073
AoxI GGCC 1 cut(s) 1063
ApeKI GCWGC 7 cut(s) 180, 512, 786, 1022, 1181, 1794, 2445
ApoI RAATTY 5 cut(s) 113, 284, 925, 1214, 1635
AsiGI ACCGGT 1 cut(s) 2429
Asp700I GAANNNNTTC 5 cut(s) 102, 214, 530, 914, 1701
AspA2I CCTAGG 1 cut(s) 2600
AspLEI GCGC 5 cut(s) 1038, 1659, 1799, 2169, 2308
AspS9I GGNCC 2 cut(s) 340, 1726
AsuHPI GGTGA 6 cut(s) 362, 863, 1240, 1792, 2320, 2653
AsuNHI GCTAGC 1 cut(s) 574
AvaI CYCGRG 1 cut(s) 1073
AvaII GGWCC 2 cut(s) 340, 1726
AvrII CCTAGG 1 cut(s) 2600
AxyI CCTNAGG 1 cut(s) 14
BaeI ACNNNNGTAYC 2 cut(s) 1539, 1572
BalI TGGCCA 1 cut(s) 1065
BanII GRGCYC 3 cut(s) 54, 900, 2511
BbsI GAAGAC 2 cut(s) 917, 1947
Bbv12I GWGCWC 2 cut(s) 54, 900
BbvI GCAGC 7 cut(s) 167, 524, 798, 1009, 1193, 1806, 2457
BccI CCATC 8 cut(s) 351, 403, 738, 1193, 1426, 1862, 2116, 2200
BceAI ACGGC 2 cut(s) 799, 2503
BciT130I CCWGG 5 cut(s) 241, 491, 1255, 1552, 1595
BciVI GTATCC 1 cut(s) 1232
BclI TGATCA 1 cut(s) 136
BcoDI GTCTC 5 cut(s) 72, 1076, 1181, 2031, 2375
BcuI ACTAGT 1 cut(s) 1822
BfaI CTAG 7 cut(s) 365, 575, 881, 1823, 2003, 2442, 2601
BfmI CTRYAG 1 cut(s) 692
BfoI RGCGCY 1 cut(s) 2170
BfrI CTTAAG 2 cut(s) 1610, 2228
BfuAI ACCTGC 1 cut(s) 314
BfuI GTATCC 1 cut(s) 1232
BglII AGATCT 1 cut(s) 2388
BisI GCNGC 8 cut(s) 181, 513, 787, 1023, 1182, 1795, 2446, 2650
BlnI CCTAGG 1 cut(s) 2600
BlsI GCNGC 8 cut(s) 182, 514, 788, 1024, 1183, 1796, 2447, 2651
Bme1390I CCNGG 5 cut(s) 241, 491, 1255, 1552, 1595
Bme18I GGWCC 2 cut(s) 340, 1726
BmeRI GACNNNNNGTC 1 cut(s) 1263
BmeT110I CYCGRG 1 cut(s) 1073
BmgBI CACGTC 1 cut(s) 443
BmgT120I GGNCC 2 cut(s) 340, 1726
BmiI GGNNCC 3 cut(s) 2056, 2428, 2438
BmrFI CCNGG 5 cut(s) 241, 491, 1255, 1552, 1595
BmrI ACTGGG 2 cut(s) 1493, 2397
BmsI GCATC 8 cut(s) 16, 34, 192, 343, 499, 739, 2102, 2474
BmtI GCTAGC 1 cut(s) 578
BmuI ACTGGG 2 cut(s) 1493, 2397
BpiI GAAGAC 2 cut(s) 917, 1947
BpmI CTGGAG 4 cut(s) 223, 867, 1237, 2201
BpuEI CTTGAG 6 cut(s) 292, 1561, 1605, 2033, 2356, 2395
Bsa29I ATCGAT 2 cut(s) 2468, 2547
BsaAI YACGTR 1 cut(s) 542
BsaJI CCNNGG 5 cut(s) 489, 919, 1551, 1786, 2600
BsaWI WCCGGW 1 cut(s) 2429
BsaXI ACNNNNNCTCC 4 cut(s) 840, 870, 887, 917
Bsc4I CCNNNNNNNGG 1 cut(s) 337
Bse118I RCCGGY 3 cut(s) 1442, 2057, 2429
Bse1I ACTGG 5 cut(s) 629, 1499, 2184, 2403, 2650
Bse21I CCTNAGG 1 cut(s) 14
Bse3DI GCAATG 3 cut(s) 81, 1304, 2293
BseBI CCWGG 5 cut(s) 241, 491, 1255, 1552, 1595
BseCI ATCGAT 2 cut(s) 2468, 2547
BseDI CCNNGG 5 cut(s) 489, 919, 1551, 1786, 2600
BseGI GGATG 6 cut(s) 727, 749, 754, 1886, 1972, 2160
BseLI CCNNNNNNNGG 1 cut(s) 337
BseMI GCAATG 3 cut(s) 81, 1304, 2293
BseMII CTCAG 6 cut(s) 5, 249, 575, 959, 1401, 1526
BseNI ACTGG 5 cut(s) 629, 1499, 2184, 2403, 2650
BseRI GAGGAG 3 cut(s) 715, 927, 1103
BseXI GCAGC 7 cut(s) 167, 524, 798, 1009, 1193, 1806, 2457
BseYI CCCAGC 2 cut(s) 1438, 1813
BsgI GTGCAG 1 cut(s) 342
Bsh1236I CGCG 1 cut(s) 1239
Bsh1285I CGRYCG 1 cut(s) 2472
BshFI GGCC 1 cut(s) 1065
BshTI ACCGGT 1 cut(s) 2429
BshVI ATCGAT 2 cut(s) 2468, 2547
BsiEI CGRYCG 1 cut(s) 2472
BsiHKAI GWGCWC 2 cut(s) 54, 900
BsiHKCI CYCGRG 1 cut(s) 1073
BsiSI CCGG 4 cut(s) 1443, 2058, 2164, 2430
BslFI GGGAC 2 cut(s) 2174, 2297
BslI CCNNNNNNNGG 1 cut(s) 337
BsmAI GTCTC 5 cut(s) 72, 1076, 1181, 2031, 2375
BsmFI GGGAC 2 cut(s) 2174, 2297
BsmI GAATGC 2 cut(s) 1615, 1979
BsnI GGCC 1 cut(s) 1065
BsoBI CYCGRG 1 cut(s) 1073
Bsp1286I GDGCHC 3 cut(s) 54, 900, 2511
BspACI CCGC 9 cut(s) 47, 277, 578, 1237, 2061, 2490, 2567, 2614, 2649
BspANI GGCC 1 cut(s) 1065
BspCNI CTCAG 6 cut(s) 6, 250, 574, 960, 1402, 1527
BspDI ATCGAT 2 cut(s) 2468, 2547
BspFNI CGCG 1 cut(s) 1239
BspHI TCATGA 1 cut(s) 2412
BspLI GGNNCC 3 cut(s) 2056, 2428, 2438
BspMI ACCTGC 1 cut(s) 314
BspOI GCTAGC 1 cut(s) 578
BspPI GGATC 6 cut(s) 251, 557, 1614, 1964, 2465, 2552
BspQI GCTCTTC 1 cut(s) 2411
BspTI CTTAAG 2 cut(s) 1610, 2228
BsrDI GCAATG 3 cut(s) 81, 1304, 2293
BsrFI RCCGGY 3 cut(s) 1442, 2057, 2429
BsrI ACTGG 5 cut(s) 629, 1499, 2184, 2403, 2650
BssAI RCCGGY 3 cut(s) 1442, 2057, 2429
BssECI CCNNGG 5 cut(s) 489, 919, 1551, 1786, 2600
BssT1I CCWWGG 3 cut(s) 919, 1786, 2600
Bst2UI CCWGG 5 cut(s) 241, 491, 1255, 1552, 1595
Bst4CI ACNGT 4 cut(s) 1070, 1405, 1630, 2641
Bst6I CTCTTC 6 cut(s) 30, 692, 1122, 1238, 2398, 2411
BstAFI CTTAAG 2 cut(s) 1610, 2228
BstBAI YACGTR 1 cut(s) 542
BstC8I GCNNGC 4 cut(s) 129, 576, 1444, 2059
BstDEI CTNAG 8 cut(s) 14, 74, 258, 561, 968, 1410, 1535, 2555
BstEII GGTNACC 1 cut(s) 326
BstENI CCTNNNNNAGG 1 cut(s) 335
BstF5I GGATG 6 cut(s) 727, 749, 754, 1886, 1972, 2160
BstFNI CGCG 1 cut(s) 1239
BstH2I RGCGCY 1 cut(s) 2170
BstHHI GCGC 5 cut(s) 1038, 1659, 1799, 2169, 2308
BstMAI GTCTC 5 cut(s) 72, 1076, 1181, 2031, 2375
BstMCI CGRYCG 1 cut(s) 2472
BstMWI GCNNNNNNNGC 4 cut(s) 403, 821, 1001, 1283
BstNI CCWGG 5 cut(s) 241, 491, 1255, 1552, 1595
BstNSI RCATGY 1 cut(s) 2312
BstPI GGTNACC 1 cut(s) 326
BstSCI CCNGG 5 cut(s) 239, 489, 1253, 1550, 1593
BstSFI CTRYAG 1 cut(s) 692
BstUI CGCG 1 cut(s) 1239
BstV1I GCAGC 7 cut(s) 167, 524, 798, 1009, 1193, 1806, 2457
BstV2I GAAGAC 2 cut(s) 917, 1947
BstX2I RGATCY 3 cut(s) 1606, 2388, 2457
BstYI RGATCY 3 cut(s) 1606, 2388, 2457
Bsu15I ATCGAT 2 cut(s) 2468, 2547
Bsu36I CCTNAGG 1 cut(s) 14
BsuI GTATCC 1 cut(s) 1232
BsuRI GGCC 1 cut(s) 1065
BsuTUI ATCGAT 2 cut(s) 2468, 2547
BtrI CACGTC 1 cut(s) 443
BtsCI GGATG 6 cut(s) 727, 749, 754, 1886, 1972, 2160
BtsI GCAGTG 2 cut(s) 764, 1343
BtsIMutI CAGTG 4 cut(s) 764, 1343, 1938, 2177
BveI ACCTGC 1 cut(s) 314
Cac8I GCNNGC 4 cut(s) 129, 576, 1444, 2059
CaiI CAGNNNCTG 2 cut(s) 1409, 1594
CciI TCATGA 1 cut(s) 2412
CfoI GCGC 5 cut(s) 1038, 1659, 1799, 2169, 2308
Cfr10I RCCGGY 3 cut(s) 1442, 2057, 2429
Cfr13I GGNCC 2 cut(s) 340, 1726
ClaI ATCGAT 2 cut(s) 2468, 2547
Csp6I GTAC 2 cut(s) 1479, 2006
CspAI ACCGGT 1 cut(s) 2429
CviQI GTAC 2 cut(s) 1479, 2006
DdeI CTNAG 8 cut(s) 14, 74, 258, 561, 968, 1410, 1535, 2555
DraI TTTAAA 2 cut(s) 1488, 1527
DriI GACNNNNNGTC 1 cut(s) 1263
EaeI YGGCCR 1 cut(s) 1063
Eam1104I CTCTTC 6 cut(s) 30, 692, 1122, 1238, 2398, 2411
Eam1105I GACNNNNNGTC 1 cut(s) 1263
EarI CTCTTC 6 cut(s) 30, 692, 1122, 1238, 2398, 2411
EciI GGCGGA 3 cut(s) 266, 2076, 2505
Ecl136II GAGCTC 2 cut(s) 52, 898
Eco130I CCWWGG 3 cut(s) 919, 1786, 2600
Eco24I GRGCYC 3 cut(s) 54, 900, 2511
Eco32I GATATC 1 cut(s) 206
Eco47I GGWCC 2 cut(s) 340, 1726
Eco53kI GAGCTC 2 cut(s) 52, 898
Eco57I CTGAAG 3 cut(s) 1571, 1914, 1970
Eco81I CCTNAGG 1 cut(s) 14
Eco88I CYCGRG 1 cut(s) 1073
Eco91I GGTNACC 1 cut(s) 326
EcoICRI GAGCTC 2 cut(s) 52, 898
EcoNI CCTNNNNNAGG 1 cut(s) 335
EcoO109I RGGNCCY 1 cut(s) 1726
EcoO65I GGTNACC 1 cut(s) 326
EcoRI GAATTC 1 cut(s) 925
EcoRII CCWGG 5 cut(s) 239, 489, 1253, 1550, 1593
EcoRV GATATC 1 cut(s) 206
EcoT14I CCWWGG 3 cut(s) 919, 1786, 2600
EcoT22I ATGCAT 3 cut(s) 31, 1918, 2117
EcoT38I GRGCYC 3 cut(s) 54, 900, 2511
ErhI CCWWGG 3 cut(s) 919, 1786, 2600
FalI AAGNNNNNCTT 4 cut(s) 90, 122, 1468, 1500
FaqI GGGAC 2 cut(s) 2174, 2297
FauNDI CATATG 2 cut(s) 31, 1714
FbaI TGATCA 1 cut(s) 136
FblI GTMKAC 1 cut(s) 2574
Fnu4HI GCNGC 8 cut(s) 181, 513, 787, 1023, 1182, 1795, 2446, 2650
FokI GGATG 6 cut(s) 734, 756, 761, 1873, 1979, 2147
FriOI GRGCYC 3 cut(s) 54, 900, 2511
Fsp4HI GCNGC 8 cut(s) 181, 513, 787, 1023, 1182, 1795, 2446, 2650
FspBI CTAG 7 cut(s) 365, 575, 881, 1823, 2003, 2442, 2601
FspI TGCGCA 1 cut(s) 2307
GlaI GCGC 5 cut(s) 1037, 1658, 1798, 2168, 2307
GluI GCNGC 8 cut(s) 181, 513, 787, 1023, 1182, 1795, 2446, 2650
GsaI CCCAGC 2 cut(s) 1442, 1817
GsuI CTGGAG 4 cut(s) 223, 867, 1237, 2201
HaeII RGCGCY 1 cut(s) 2170
HaeIII GGCC 1 cut(s) 1065
HapII CCGG 4 cut(s) 1443, 2058, 2164, 2430
HhaI GCGC 5 cut(s) 1038, 1659, 1799, 2169, 2308
Hin6I GCGC 5 cut(s) 1036, 1657, 1797, 2167, 2306
HinP1I GCGC 5 cut(s) 1036, 1657, 1797, 2167, 2306
HincII GTYRAC 1 cut(s) 2575
HindII GTYRAC 1 cut(s) 2575
HindIII AAGCTT 2 cut(s) 170, 1583
HinfI GANTC 6 cut(s) 273, 842, 887, 1174, 1513, 2263
HpaII CCGG 4 cut(s) 1443, 2058, 2164, 2430
HphI GGTGA 6 cut(s) 362, 863, 1240, 1792, 2320, 2653
Hpy166II GTNNAC 6 cut(s) 440, 1401, 1481, 1783, 2575, 2656
Hpy188III TCNNGA 9 cut(s) 13, 89, 107, 187, 365, 846, 2012, 2260, 2413
Hpy8I GTNNAC 6 cut(s) 440, 1401, 1481, 1783, 2575, 2656
Hpy99I CGWCG 2 cut(s) 1326, 2489
HpyAV CCTTC 6 cut(s) 131, 1197, 1766, 1830, 1994, 2027
HpyCH4III ACNGT 4 cut(s) 1070, 1405, 1630, 2641
HpyCH4IV ACGT 3 cut(s) 442, 541, 1384
HpyF10VI GCNNNNNNNGC 4 cut(s) 403, 821, 1001, 1283
HpyF3I CTNAG 8 cut(s) 14, 74, 258, 561, 968, 1410, 1535, 2555
HpySE526I ACGT 3 cut(s) 442, 541, 1384
HspAI GCGC 5 cut(s) 1036, 1657, 1797, 2167, 2306
KroI GCCGGC 2 cut(s) 1442, 2057
KroNI GCCGGC 2 cut(s) 1444, 2059
Ksp22I TGATCA 1 cut(s) 136
LguI GCTCTTC 1 cut(s) 2411
LmnI GCTCC 9 cut(s) 49, 317, 612, 895, 903, 1167, 1742, 2054, 2602
Lsp1109I GCAGC 7 cut(s) 167, 524, 798, 1009, 1193, 1806, 2457
LweI GCATC 8 cut(s) 16, 34, 192, 343, 499, 739, 2102, 2474
MaeI CTAG 7 cut(s) 365, 575, 881, 1823, 2003, 2442, 2601
MaeII ACGT 3 cut(s) 442, 541, 1384
MaeIII GTNAC 8 cut(s) 222, 326, 956, 1207, 1300, 1624, 2326, 2641
MflI RGATCY 3 cut(s) 1606, 2388, 2457
MhlI GDGCHC 3 cut(s) 54, 900, 2511
MlsI TGGCCA 1 cut(s) 1065
MluNI TGGCCA 1 cut(s) 1065
MlyI GAGTC 3 cut(s) 267, 896, 2257
MmeI TCCRAC 4 cut(s) 315, 1672, 2414, 2508
Mox20I TGGCCA 1 cut(s) 1065
Mph1103I ATGCAT 3 cut(s) 31, 1918, 2117
MroNI GCCGGC 2 cut(s) 1442, 2057
MroXI GAANNNNTTC 5 cut(s) 102, 214, 530, 914, 1701
MscI TGGCCA 1 cut(s) 1065
MslI CAYNNNNRTG 8 cut(s) 93, 138, 348, 738, 1778, 2120, 2280, 2286
Msp20I TGGCCA 1 cut(s) 1065
MspA1I CMGCKG 2 cut(s) 667, 2567
MspCI CTTAAG 2 cut(s) 1610, 2228
MspI CCGG 4 cut(s) 1443, 2058, 2164, 2430
MspR9I CCNGG 5 cut(s) 241, 491, 1255, 1552, 1595
Mva1269I GAATGC 2 cut(s) 1615, 1979
MvaI CCWGG 5 cut(s) 241, 491, 1255, 1552, 1595
MvnI CGCG 1 cut(s) 1239
MwoI GCNNNNNNNGC 4 cut(s) 403, 821, 1001, 1283
NaeI GCCGGC 2 cut(s) 1444, 2059
NdeI CATATG 2 cut(s) 31, 1714
NgoMIV GCCGGC 2 cut(s) 1442, 2057
NheI GCTAGC 1 cut(s) 574
NlaIV GGNNCC 3 cut(s) 2056, 2428, 2438
NmuCI GTSAC 3 cut(s) 1207, 2326, 2641
NsbI TGCGCA 1 cut(s) 2307
NsiI ATGCAT 3 cut(s) 31, 1918, 2117
NspI RCATGY 1 cut(s) 2312
OliI CACNNNNGTG 1 cut(s) 93
PagI TCATGA 1 cut(s) 2412
PciSI GCTCTTC 1 cut(s) 2411
PcsI WCGNNNNNNNCGW 1 cut(s) 2469
PctI GAATGC 2 cut(s) 1615, 1979
PdiI GCCGGC 2 cut(s) 1444, 2059
PdmI GAANNNNTTC 5 cut(s) 102, 214, 530, 914, 1701
PfeI GAWTC 3 cut(s) 842, 1174, 1513
PfoI TCCNGGA 2 cut(s) 239, 1253
PinAI ACCGGT 1 cut(s) 2429
PkrI GCNGC 8 cut(s) 182, 514, 788, 1024, 1183, 1796, 2447, 2651
Ple19I CGATCG 1 cut(s) 2472
PleI GAGTC 3 cut(s) 267, 895, 2257
PpsI GAGTC 3 cut(s) 267, 895, 2257
Ppu21I YACGTR 1 cut(s) 542
PpuMI RGGWCCY 1 cut(s) 1726
Psp124BI GAGCTC 2 cut(s) 54, 900
Psp5II RGGWCCY 1 cut(s) 1726
Psp6I CCWGG 5 cut(s) 239, 489, 1253, 1550, 1593
PspEI GGTNACC 1 cut(s) 326
PspFI CCCAGC 2 cut(s) 1438, 1813
PspGI CCWGG 5 cut(s) 239, 489, 1253, 1550, 1593
PspN4I GGNNCC 3 cut(s) 2056, 2428, 2438
PspPI GGNCC 2 cut(s) 340, 1726
PspPPI RGGWCCY 1 cut(s) 1726
PstNI CAGNNNCTG 2 cut(s) 1409, 1594
PsuI RGATCY 3 cut(s) 1606, 2388, 2457
PvuI CGATCG 1 cut(s) 2472
PvuII CAGCTG 1 cut(s) 667
RsaI GTAC 2 cut(s) 1480, 2007
RsaNI GTAC 2 cut(s) 1479, 2006
RseI CAYNNNNRTG 8 cut(s) 93, 138, 348, 738, 1778, 2120, 2280, 2286
SacI GAGCTC 2 cut(s) 54, 900
SalI GTCGAC 1 cut(s) 2573
SapI GCTCTTC 1 cut(s) 2411
SatI GCNGC 8 cut(s) 181, 513, 787, 1023, 1182, 1795, 2446, 2650
Sau96I GGNCC 2 cut(s) 340, 1726
SchI GAGTC 3 cut(s) 267, 896, 2257
ScrFI CCNGG 5 cut(s) 241, 491, 1255, 1552, 1595
SduI GDGCHC 3 cut(s) 54, 900, 2511
SfaNI GCATC 8 cut(s) 16, 34, 192, 343, 499, 739, 2102, 2474
SfcI CTRYAG 1 cut(s) 692
SinI GGWCC 2 cut(s) 340, 1726
SmiMI CAYNNNNRTG 8 cut(s) 93, 138, 348, 738, 1778, 2120, 2280, 2286
SmlI CTYRAG 8 cut(s) 307, 1540, 1610, 1620, 2012, 2228, 2335, 2374
SmoI CTYRAG 8 cut(s) 307, 1540, 1610, 1620, 2012, 2228, 2335, 2374
SpeI ACTAGT 1 cut(s) 1822
SsiI CCGC 9 cut(s) 47, 277, 578, 1237, 2061, 2490, 2567, 2614, 2649
SspMI CTAG 7 cut(s) 365, 575, 881, 1823, 2003, 2442, 2601
SstI GAGCTC 2 cut(s) 54, 900
StyD4I CCNGG 5 cut(s) 239, 489, 1253, 1550, 1593
StyI CCWWGG 3 cut(s) 919, 1786, 2600
TaaI ACNGT 4 cut(s) 1070, 1405, 1630, 2641
TaiI ACGT 3 cut(s) 445, 544, 1387
TatI WGTACW 2 cut(s) 1478, 2005
TauI GCSGC 1 cut(s) 2652
TfiI GAWTC 3 cut(s) 842, 1174, 1513
TscAI CASTG 4 cut(s) 771, 1350, 1938, 2184
TseFI GTSAC 3 cut(s) 1207, 2326, 2641
TseI GCWGC 7 cut(s) 180, 512, 786, 1022, 1181, 1794, 2445
Tsp45I GTSAC 3 cut(s) 1207, 2326, 2641
TspGWI ACGGA 3 cut(s) 854, 1310, 2512
TspRI CASTG 4 cut(s) 771, 1350, 1938, 2184
Vha464I CTTAAG 2 cut(s) 1610, 2228
VpaK11BI GGWCC 2 cut(s) 340, 1726
XagI CCTNNNNNAGG 1 cut(s) 335
XapI RAATTY 5 cut(s) 113, 284, 925, 1214, 1635
XbaI TCTAGA 1 cut(s) 364
XceI RCATGY 1 cut(s) 2312
XcmI CCANNNNNNNNNTGG 1 cut(s) 1930
XmaJI CCTAGG 1 cut(s) 2600
XmiI GTMKAC 1 cut(s) 2574
XmnI GAANNNNTTC 5 cut(s) 102, 214, 530, 914, 1701
XspI CTAG 7 cut(s) 365, 575, 881, 1823, 2003, 2442, 2601
Zsp2I ATGCAT 3 cut(s) 31, 1918, 2117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.