RchiOBHm_Chr5g0026041

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001297
Physical Location & Seq
Forward (+)
20029896 .. 20032375
2480 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30564

Sequence Viewer

Length: 900 bp
ATGGCATCACCCCAGCTACGGCTACCAGGAGGCTTTGAGAGCTCTAGCTCTCTTATGAGCATCCTGGAGCTCACCAAAGAGTCATTAACTGAAGTCCCACAATGTTACGTCCGTAGTTTCGAAGAACCAGAACTTTCATCTGATCTCTCTGATAAGCCCACCGTAATCCCAACCATCGATATGATAAAACTAGTAACGGGATCAGAAGCTTCGGATTCTGAACTGGAAAATCTGCGCTCAACTTGCACAGAGTGGGGAATCTTTCAGTTGGTGAACCATGGAGTTAGCCCTATGCTTTTGGAGAAGCTGAAGTACGAGGTTGAAGAATTCTTTAAGCTTCCCTTGGAAGAGAAAATGAAGTATCAGATAAGACCTGGTGACGTTGAAGGCTATGGATCCATAGCCTATAAAGAGCAAAAACGTGACTGGGGTAATAGATTCTACATGACCATGAACCCTGTTCATAGGAGAAAGCCATACCTCTTCCCAGAGCTCCCTTCATCCCTCAGGAATACCTTCGAGTCATACTACTTGGAACTGAAGCAACTAGCCATGGAACTTATTGGGTTTCTGGGGAAGGCTATGAAGATAGACATAAAAGAGACTGAGGAGATGTTTGAAGATGGGATGCAGTCAGTGAGGATGAATTACTATCCTGCATGCCCACAACCAGAGCTGGTCATCGGCCTCACACCTCACTCGGACGCTTCCGGGATCACCATCCTCCACCAGCTTAACGGGGTGGGTGGTCTGCAGATTAAGAAAGATGGGGTTTGGGTTCCTGTCAACTTCCGTGAAGATGCCTTTGTGGTTAATGTAGGGGACATCTTTGAGGTTTGTCCTATTTACCCTTCTTTCCACTCAACCAAAATAAAATCACTAAACTATTCTATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

299

Amino Acids

34.06

Weight (kDa)

5.02

Isoelectric Point (pI)

54.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 56 - 155 1.7e-25 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 211 - 280 6.6e-21 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 208, 390, 403, 722
AcsI RAATTY 1 cut(s) 326
AcuI CTGAAG 3 cut(s) 111, 329, 560
AdeI CACNNNGTG 1 cut(s) 252
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 1 cut(s) 18
AgsI TTSAA 3 cut(s) 323, 386, 620
AhlI ACTAGT 1 cut(s) 190
AjnI CCWGG 3 cut(s) 25, 63, 373
Alw21I GWGCWC 3 cut(s) 44, 72, 495
Alw26I GTCTC 1 cut(s) 596
AlwI GGATC 4 cut(s) 208, 390, 403, 722
AoxI GGCC 1 cut(s) 685
ApoI RAATTY 1 cut(s) 326
AseI ATTAAT 1 cut(s) 894
Asp700I GAANNNNTTC 1 cut(s) 515
AspLEI GCGC 1 cut(s) 237
AsuC2I CCSGG 1 cut(s) 712
AsuHPI GGTGA 4 cut(s) 64, 283, 389, 709
AsuII TTCGAA 1 cut(s) 120
AxyI CCTNAGG 1 cut(s) 506
BamHI GGATCC 1 cut(s) 395
BanII GRGCYC 3 cut(s) 44, 72, 495
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
Bbv12I GWGCWC 3 cut(s) 44, 72, 495
BccI CCATC 4 cut(s) 182, 617, 728, 761
BceAI ACGGC 1 cut(s) 35
BciT130I CCWGG 3 cut(s) 27, 65, 375
BcnI CCSGG 1 cut(s) 712
BcoDI GTCTC 1 cut(s) 596
BcuI ACTAGT 1 cut(s) 190
BfaI CTAG 3 cut(s) 45, 191, 548
BfmI CTRYAG 1 cut(s) 752
Bme1390I CCNGG 4 cut(s) 27, 65, 375, 712
BmiI GGNNCC 2 cut(s) 397, 780
BmrFI CCNGG 4 cut(s) 27, 65, 375, 712
BmrI ACTGGG 1 cut(s) 436
BmsI GCATC 4 cut(s) 14, 69, 618, 790
BmuI ACTGGG 1 cut(s) 436
BpmI CTGGAG 1 cut(s) 86
Bpu14I TTCGAA 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 712
Bsa29I ATCGAT 1 cut(s) 177
BsaBI GATNNNNATC 1 cut(s) 719
BsaJI CCNNGG 3 cut(s) 277, 342, 552
Bsc4I CCNNNNNNNGG 1 cut(s) 18
Bse1I ACTGG 2 cut(s) 228, 431
Bse21I CCTNAGG 1 cut(s) 506
Bse8I GATNNNNATC 1 cut(s) 719
BseBI CCWGG 3 cut(s) 27, 65, 375
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 3 cut(s) 277, 342, 552
BseGI GGATG 5 cut(s) 60, 500, 633, 648, 720
BseJI GATNNNNATC 1 cut(s) 719
BseLI CCNNNNNNNGG 1 cut(s) 18
BseMII CTCAG 2 cut(s) 520, 597
BseNI ACTGG 2 cut(s) 228, 431
BseRI GAGGAG 1 cut(s) 623
BseYI CCCAGC 1 cut(s) 12
BshFI GGCC 1 cut(s) 687
BshVI ATCGAT 1 cut(s) 177
BsiHKAI GWGCWC 3 cut(s) 44, 72, 495
BsiSI CCGG 1 cut(s) 711
BslFI GGGAC 2 cut(s) 80, 836
BslI CCNNNNNNNGG 1 cut(s) 18
BsmAI GTCTC 1 cut(s) 596
BsmFI GGGAC 2 cut(s) 80, 836
BsnI GGCC 1 cut(s) 687
Bsp119I TTCGAA 1 cut(s) 120
Bsp1286I GDGCHC 3 cut(s) 44, 72, 495
Bsp143I GATC 4 cut(s) 142, 200, 395, 714
Bsp19I CCATGG 2 cut(s) 277, 552
BspANI GGCC 1 cut(s) 687
BspCNI CTCAG 2 cut(s) 519, 598
BspDI ATCGAT 1 cut(s) 177
BspLI GGNNCC 2 cut(s) 397, 780
BspMAI CTGCAG 1 cut(s) 756
BspPI GGATC 4 cut(s) 208, 390, 403, 722
BspT104I TTCGAA 1 cut(s) 120
BsrI ACTGG 2 cut(s) 228, 431
BssECI CCNNGG 3 cut(s) 277, 342, 552
BssMI GATC 4 cut(s) 142, 200, 395, 714
BssT1I CCWWGG 3 cut(s) 277, 342, 552
Bst2UI CCWGG 3 cut(s) 27, 65, 375
Bst4CI ACNGT 1 cut(s) 163
Bst6I CTCTTC 2 cut(s) 342, 488
BstBI TTCGAA 1 cut(s) 120
BstC8I GCNNGC 1 cut(s) 661
BstDEI CTNAG 2 cut(s) 506, 606
BstDSI CCRYGG 2 cut(s) 277, 552
BstF5I GGATG 5 cut(s) 60, 500, 633, 648, 720
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 4 cut(s) 145, 203, 398, 717
BstMAI GTCTC 1 cut(s) 596
BstMBI GATC 4 cut(s) 142, 200, 395, 714
BstMWI GCNNNNNNNGC 2 cut(s) 39, 243
BstNI CCWGG 3 cut(s) 27, 65, 375
BstNSI RCATGY 1 cut(s) 663
BstSCI CCNGG 4 cut(s) 25, 63, 373, 710
BstSFI CTRYAG 1 cut(s) 752
BstX2I RGATCY 1 cut(s) 395
BstYI RGATCY 1 cut(s) 395
Bsu15I ATCGAT 1 cut(s) 177
Bsu36I CCTNAGG 1 cut(s) 506
BsuRI GGCC 1 cut(s) 687
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 2 cut(s) 277, 552
BtsCI GGATG 5 cut(s) 60, 500, 633, 648, 720
BtsIMutI CAGTG 1 cut(s) 642
Cac8I GCNNGC 1 cut(s) 661
CfoI GCGC 1 cut(s) 237
ClaI ATCGAT 1 cut(s) 177
CseI GACGC 1 cut(s) 713
CsiI ACCWGGT 1 cut(s) 373
Csp6I GTAC 1 cut(s) 313
CviAII CATG 5 cut(s) 278, 445, 451, 553, 660
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 2 cut(s) 506, 606
DpnI GATC 4 cut(s) 144, 202, 397, 716
DpnII GATC 4 cut(s) 142, 200, 395, 714
DraIII CACNNNGTG 1 cut(s) 252
Eam1104I CTCTTC 2 cut(s) 342, 488
EarI CTCTTC 2 cut(s) 342, 488
Ecl136II GAGCTC 3 cut(s) 42, 70, 493
Eco130I CCWWGG 3 cut(s) 277, 342, 552
Eco24I GRGCYC 3 cut(s) 44, 72, 495
Eco53kI GAGCTC 3 cut(s) 42, 70, 493
Eco57I CTGAAG 3 cut(s) 111, 329, 560
Eco81I CCTNAGG 1 cut(s) 506
EcoICRI GAGCTC 3 cut(s) 42, 70, 493
EcoRI GAATTC 1 cut(s) 326
EcoRII CCWGG 3 cut(s) 25, 63, 373
EcoT14I CCWWGG 3 cut(s) 277, 342, 552
EcoT38I GRGCYC 3 cut(s) 44, 72, 495
ErhI CCWWGG 3 cut(s) 277, 342, 552
FaeI CATG 5 cut(s) 281, 448, 454, 556, 663
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FaqI GGGAC 2 cut(s) 80, 836
FatI CATG 5 cut(s) 277, 444, 450, 552, 659
FokI GGATG 5 cut(s) 47, 487, 640, 655, 707
FriOI GRGCYC 3 cut(s) 44, 72, 495
FspBI CTAG 3 cut(s) 45, 191, 548
GlaI GCGC 1 cut(s) 236
GsaI CCCAGC 1 cut(s) 16
GsuI CTGGAG 1 cut(s) 86
HaeIII GGCC 1 cut(s) 687
HapII CCGG 1 cut(s) 711
HgaI GACGC 1 cut(s) 713
HhaI GCGC 1 cut(s) 237
Hin1II CATG 5 cut(s) 281, 448, 454, 556, 663
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HincII GTYRAC 1 cut(s) 787
HindII GTYRAC 1 cut(s) 787
HindIII AAGCTT 2 cut(s) 207, 335
HinfI GANTC 5 cut(s) 80, 215, 258, 438, 521
HpaII CCGG 1 cut(s) 711
HphI GGTGA 4 cut(s) 64, 283, 389, 709
Hpy166II GTNNAC 2 cut(s) 274, 787
Hpy188I TCNGA 7 cut(s) 142, 151, 205, 214, 220, 366, 703
Hpy188III TCNNGA 1 cut(s) 508
Hpy8I GTNNAC 2 cut(s) 274, 787
HpyAV CCTTC 5 cut(s) 380, 507, 526, 571, 861
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4IV ACGT 3 cut(s) 108, 381, 421
HpyCH4V TGCA 4 cut(s) 246, 631, 659, 754
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 243
HpyF3I CTNAG 2 cut(s) 506, 606
HpySE526I ACGT 3 cut(s) 108, 381, 421
Hsp92II CATG 5 cut(s) 281, 448, 454, 556, 663
HspAI GCGC 1 cut(s) 235
Kzo9I GATC 4 cut(s) 142, 200, 395, 714
LmnI GCTCC 2 cut(s) 67, 498
LweI GCATC 4 cut(s) 14, 69, 618, 790
MabI ACCWGGT 1 cut(s) 373
MaeI CTAG 3 cut(s) 45, 191, 548
MaeII ACGT 3 cut(s) 108, 381, 421
MaeIII GTNAC 4 cut(s) 104, 193, 377, 422
MalI GATC 4 cut(s) 144, 202, 397, 716
MboI GATC 4 cut(s) 142, 200, 395, 714
MboII GAAGA 7 cut(s) 134, 335, 359, 475, 598, 632, 809
MflI RGATCY 1 cut(s) 395
MhlI GDGCHC 3 cut(s) 44, 72, 495
MluCI AATT 3 cut(s) 326, 646, 895
MlyI GAGTC 2 cut(s) 89, 530
MroXI GAANNNNTTC 1 cut(s) 515
MseI TTAA 7 cut(s) 86, 333, 735, 759, 813, 894, 898
MslI CAYNNNNRTG 2 cut(s) 179, 449
MspI CCGG 1 cut(s) 711
MspR9I CCNGG 4 cut(s) 27, 65, 375, 712
MvaI CCWGG 3 cut(s) 27, 65, 375
MwoI GCNNNNNNNGC 2 cut(s) 39, 243
NciI CCSGG 1 cut(s) 712
NcoI CCATGG 2 cut(s) 277, 552
NdeII GATC 4 cut(s) 142, 200, 395, 714
NlaIII CATG 5 cut(s) 281, 448, 454, 556, 663
NlaIV GGNNCC 2 cut(s) 397, 780
NmuCI GTSAC 2 cut(s) 377, 422
NspI RCATGY 1 cut(s) 663
NspV TTCGAA 1 cut(s) 120
PacI TTAATTAA 1 cut(s) 898
PaeI GCATGC 1 cut(s) 663
PdmI GAANNNNTTC 1 cut(s) 515
PfeI GAWTC 3 cut(s) 215, 258, 438
PfoI TCCNGGA 2 cut(s) 63, 710
PleI GAGTC 2 cut(s) 88, 529
PpsI GAGTC 2 cut(s) 88, 529
PshBI ATTAAT 1 cut(s) 894
Psp124BI GAGCTC 3 cut(s) 44, 72, 495
Psp6I CCWGG 3 cut(s) 25, 63, 373
PspFI CCCAGC 1 cut(s) 12
PspGI CCWGG 3 cut(s) 25, 63, 373
PspN4I GGNNCC 2 cut(s) 397, 780
PstI CTGCAG 1 cut(s) 756
PsuI RGATCY 1 cut(s) 395
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
RseI CAYNNNNRTG 2 cut(s) 179, 449
SacI GAGCTC 3 cut(s) 44, 72, 495
SaqAI TTAA 7 cut(s) 86, 333, 735, 759, 813, 894, 898
Sau3AI GATC 4 cut(s) 142, 200, 395, 714
SchI GAGTC 2 cut(s) 89, 530
ScrFI CCNGG 4 cut(s) 27, 65, 375, 712
SduI GDGCHC 3 cut(s) 44, 72, 495
SexAI ACCWGGT 1 cut(s) 373
SfaNI GCATC 4 cut(s) 14, 69, 618, 790
SfcI CTRYAG 1 cut(s) 752
SfuI TTCGAA 1 cut(s) 120
SmiMI CAYNNNNRTG 2 cut(s) 179, 449
SpeI ACTAGT 1 cut(s) 190
SphI GCATGC 1 cut(s) 663
Sse9I AATT 3 cut(s) 326, 646, 895
SspMI CTAG 3 cut(s) 45, 191, 548
SstI GAGCTC 3 cut(s) 44, 72, 495
StyD4I CCNGG 4 cut(s) 25, 63, 373, 710
StyI CCWWGG 3 cut(s) 277, 342, 552
TaaI ACNGT 1 cut(s) 163
TaiI ACGT 3 cut(s) 111, 384, 424
TaqI TCGA 3 cut(s) 120, 177, 519
TasI AATT 3 cut(s) 326, 646, 895
TfiI GAWTC 3 cut(s) 215, 258, 438
Tru1I TTAA 7 cut(s) 86, 333, 735, 759, 813, 894, 898
Tru9I TTAA 7 cut(s) 86, 333, 735, 759, 813, 894, 898
TscAI CASTG 1 cut(s) 642
TseFI GTSAC 2 cut(s) 377, 422
Tsp45I GTSAC 2 cut(s) 377, 422
TspDTI ATGAA 7 cut(s) 126, 371, 452, 467, 489, 599, 659
TspGWI ACGGA 2 cut(s) 101, 782
TspRI CASTG 1 cut(s) 642
VspI ATTAAT 1 cut(s) 894
XapI RAATTY 1 cut(s) 326
XceI RCATGY 1 cut(s) 663
XmnI GAANNNNTTC 1 cut(s) 515
XspI CTAG 3 cut(s) 45, 191, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.