Rorug05G0003900

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001297
Physical Location & Seq
Forward (+)
256904 .. 264089
7186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0003900.1

Sequence Viewer

Length: 2322 bp
ATGCTTGCTGGAGTGAAGTTCATACCTCGAGACCAGATTGATAAAGATGAGAATTTGAATGCTTCTGCAAAACAGAAACAGAAGAAGAGCTCAGGTAATAAGAAGGGTAAGCGTAGGAGGGAAAGGAGTTCTGGATATAGTAGCTCGGATGGCGAAGAGCTTGAGAGAATAAAGAAGGGATCTAAAAAAAAGTGGTATTCATCGGATGAGTACTCATCATCATCATCATATTCTTCGGGAAATGAAAGTGAGGAAAGTTCTGACCGAGATAGAAAGAAGCGTCTGAATAAGAAAAAGAAGAGATCGAGTAGCAGTAGAAAAGATTATAGTTCAGAAGATCTTTCATCTGCTTCTGATAGGAAAGACAAGCGCAAGAAGAGAAGCCGAAAAGATGGAAAGAAAAAGAGTAGAGATAGAAGTCAGGGTGGCATGGAGGATGATGAATATTCTGACGGTGGTAGAGGAACTAGTACGGTGAAAGATGGGGAAATTGTGAGGAAGAAAATTGGGTTGGAGTGGATGCTTAGACCTGAAGGAAAAGAGAGAACACCTACTATTACTGTCGAGGACCAACCAAAAGAAACTCCAGTTGAAGAGGTCAATCCTAGGGAATTAAATCCATATTTTAAGGATGAGGGAAGTGGTTATCCAGAAGACAATGATGAACCTAAAGCTGGTGGGGACCGACTTTTACCTTCCTCTGTTGTTGGGGATGGAGGTGCAAGTTGGAGACTTAAAGCATTGAAGCGGGCAAAAGAGCAAGCAACTCGAGAAGGACAAGGGCTCCACGAGGTTGTTGAGGAGCGTTGGGGTTCTCTTGGTAAACTAGCGGCCTCTGTGGGATCAAATAAAGCAGCCCCATCTCGTGCTCATCTCCATGCAATACAAAGTAGAAAGAAAGGGGTGAGTGAGGAACAGCAGCCAGGTTCCAACAATGACATAGATAAGAAGGACACTAGCCGGCCCTACCTAAAGGATGTATCTATCCGACATCCCAAAATGAAGGAGCCTAAAGTTCAAGGTTCTTTATCCTGGGGAAAGCAAAAGGGTCAAAAGAGTCGGGATGTCTCTACCAAGGACTCTGCTCTTCTAGCGGATGCAGTGTCTAGCATAAACAGGTTTTCCAATGATGGAAGCTTCCTGAGTGAACTTATTCATAAGCAGACTAATGATTCTAGGAATCCTGTTAACGAAAATGTACTCGACACGAGAAAACCCAGCGAAGCCAGTGCTGCTGCCTCCAAGGGTGAACTGAGTGCGAACCAGTTGGCAGCTAAGGCTTTTCAGCTTCGTATGAAAGGGAAGCATGAAGAAGCTGAAGAACTTCTGCAAGAAGTAGAAAACCTAAAGGCAAATCAGGGTGGTGGAGATAATCCGATGAGACCACAATATGAAAGAGGCACAAACAGATATGCTATGCAGGATATGTCTCTTCGTCAAAAGAAAAAGGATGATGATGGTGACATGCATCTAGCCCAAAAGATAATGCAGAACAAAAAATATAGTATATCCGGTCAGGCAGATGATGAATACGACTTTGATGATGGTCCAATCAGAAAGTCTAAAAAGAAGCAGGGAAGTGATGATCGCAAGGTAACCCAGAAGAATACATTTGCAAATCGTTTCTCCATTCAGCAAGAACGCTGCCTTTTCTGCTTTGAAAATCCAAAGAGACCAGCGCATCTGGTTGTGGCAATTGGAAATTATTCATATTTGATGTTGCCACAGCAGAAGCCTGTTGTGGATGGTCATTGCTACATTTTGCCGATGCAGCATGTACCAGCCACAAGAACTCTTGATGATCATGTGTGGGAAGAAATTCGAAATTTCAAGAAATGCCTTATTATGATGTTTGCGAAGCAAGAGAAGGATGTTGTCTTCCTTGAAACAGTGATGGGGTTAGCTCAACAGCGCCGCCACTGTATAATTGAGTGTATTCCTTTGCCCCAGGATATAGCTAAAGACGCTCCCTTATATTTTAAAAAGGCAATTGATGAAGCTGAAGATGAGTGGAGCCAACACAATGCCAAAAAACTTATTGATACAAGTGTAAAGGGATTGCGAGGTTCAGTTCCAGCTAATTTTCCATATTTCCACGTGGAGTTTGGTTTAAAGAAGGGGTTTGTCCACGTGATTGATGATGAAACACAGTTCAATAGCAGCCTTGGCCTGAATGTTATAAGAGGCATGCTAGAGTTGCCTGCAGAAGACATGCATCGCCGTGGACGATATGAACCAGTGGAAGCGCAAAAGCAAGCCGTTAAGAGGTTTGATCAAGATTGGGCGCCGTTTGACTGGACAAAACAGCTTCATCAATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

773

Amino Acids

87.61

Weight (kDa)

9.3

Isoelectric Point (pI)

49.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CwfJ_C_1 PF04677 541 - 661 1.3e-42 Protein similar to CwfJ C-terminal
CwfJ_C_2 PF04676 670 - 767 6e-27 Protein similar to CwfJ C-terminus 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2180
AccB1I GGYRCC 1 cut(s) 2284
AciI CCGC 4 cut(s) 748, 830, 1094, 1915
AclWI GGATC 2 cut(s) 187, 850
AcsI RAATTY 3 cut(s) 52, 1818, 1825
AcuI CTGAAG 3 cut(s) 552, 1338, 2022
AcvI CACGTG 2 cut(s) 2098, 2131
AcyI GRCGYC 1 cut(s) 2285
AfaI GTAC 4 cut(s) 212, 472, 1200, 1779
AfiI CCNNNNNNNGG 2 cut(s) 674, 2265
AgsI TTSAA 8 cut(s) 58, 593, 745, 1019, 1661, 1831, 1886, 2155
AhlI ACTAGT 1 cut(s) 467
AjnI CCWGG 3 cut(s) 922, 1031, 1947
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
Alw21I GWGCWC 2 cut(s) 92, 871
Alw26I GTCTC 6 cut(s) 24, 724, 1072, 1375, 1434, 1666
AlwI GGATC 2 cut(s) 187, 850
Ama87I CYCGRG 2 cut(s) 27, 768
AoxI GGCC 3 cut(s) 831, 962, 2167
ApeKI GCWGC 8 cut(s) 854, 919, 1232, 1235, 1271, 1644, 1771, 2160
ApoI RAATTY 3 cut(s) 52, 1818, 1825
Asp700I GAANNNNTTC 4 cut(s) 1152, 1323, 1705, 1818
AspA2I CCTAGG 1 cut(s) 605
AspLEI GCGC 5 cut(s) 372, 1681, 1914, 2248, 2287
AspS9I GGNCC 4 cut(s) 568, 682, 963, 1547
AsuHPI GGTGA 4 cut(s) 487, 916, 1259, 1472
AsuII TTCGAA 1 cut(s) 1822
AvaI CYCGRG 2 cut(s) 27, 768
AvaII GGWCC 3 cut(s) 568, 682, 1547
AvrII CCTAGG 1 cut(s) 605
BaeI ACNNNNGTAYC 2 cut(s) 2034, 2067
BanI GGYRCC 1 cut(s) 2284
BanII GRGCYC 2 cut(s) 92, 786
BauI CACGAG 3 cut(s) 788, 864, 1207
BbrPI CACGTG 2 cut(s) 2098, 2131
BbsI GAAGAC 3 cut(s) 660, 1870, 2214
Bbv12I GWGCWC 2 cut(s) 92, 871
BbvI GCAGC 8 cut(s) 866, 931, 1219, 1222, 1283, 1631, 1783, 2172
BceAI ACGGC 3 cut(s) 2205, 2243, 2272
BcgI CGANNNNNNTGC 4 cut(s) 749, 783, 1211, 1245
BciT130I CCWGG 3 cut(s) 924, 1033, 1949
BclI TGATCA 2 cut(s) 1801, 2272
BcoDI GTCTC 6 cut(s) 24, 724, 1072, 1375, 1434, 1666
BcuI ACTAGT 1 cut(s) 467
BfaI CTAG 9 cut(s) 468, 606, 827, 957, 1091, 1107, 1176, 1472, 2192
BfmI CTRYAG 1 cut(s) 2202
BfoI RGCGCY 2 cut(s) 1915, 2288
BglII AGATCT 1 cut(s) 337
BlnI CCTAGG 1 cut(s) 605
BmcAI AGTACT 1 cut(s) 212
Bme1390I CCNGG 3 cut(s) 924, 1033, 1949
Bme18I GGWCC 3 cut(s) 568, 682, 1547
BmeT110I CYCGRG 2 cut(s) 27, 768
BmgT120I GGNCC 4 cut(s) 568, 682, 963, 1547
BmiI GGNNCC 6 cut(s) 683, 785, 928, 1008, 2015, 2286
BmrFI CCNGG 3 cut(s) 924, 1033, 1949
BmsI GCATC 6 cut(s) 510, 1087, 1477, 1690, 1758, 2224
BpiI GAAGAC 3 cut(s) 660, 1870, 2214
BpmI CTGGAG 2 cut(s) 30, 570
Bpu10I CCTNAGC 2 cut(s) 91, 1275
Bpu14I TTCGAA 1 cut(s) 1822
BpuEI CTTGAG 1 cut(s) 182
BsaAI YACGTR 2 cut(s) 2098, 2131
BsaHI GRCGYC 1 cut(s) 2285
BsaI GGTCTC 3 cut(s) 24, 1375, 1666
BsaJI CCNNGG 7 cut(s) 605, 1032, 1074, 1242, 1947, 2164, 2221
BsaWI WCCGGW 1 cut(s) 1511
BsaXI ACNNNNNCTCC 2 cut(s) 768, 798
Bsc4I CCNNNNNNNGG 2 cut(s) 674, 2265
Bse118I RCCGGY 1 cut(s) 960
Bse1I ACTGG 5 cut(s) 587, 1227, 1264, 2237, 2300
Bse3DI GCAATG 1 cut(s) 1750
BseBI CCWGG 3 cut(s) 924, 1033, 1949
BseDI CCNNGG 7 cut(s) 605, 1032, 1074, 1242, 1947, 2164, 2221
BseLI CCNNNNNNNGG 2 cut(s) 674, 2265
BseMI GCAATG 1 cut(s) 1750
BseMII CTCAG 3 cut(s) 105, 1133, 1244
BseNI ACTGG 5 cut(s) 587, 1227, 1264, 2237, 2300
BseRI GAGGAG 1 cut(s) 815
BseXI GCAGC 8 cut(s) 866, 931, 1219, 1222, 1283, 1631, 1783, 2172
BseYI CCCAGC 1 cut(s) 1217
BshFI GGCC 3 cut(s) 833, 964, 2169
BshNI GGYRCC 1 cut(s) 2284
BsiHKAI GWGCWC 2 cut(s) 92, 871
BsiHKCI CYCGRG 2 cut(s) 27, 768
BsiSI CCGG 2 cut(s) 961, 1512
BslFI GGGAC 1 cut(s) 695
BslI CCNNNNNNNGG 2 cut(s) 674, 2265
BsmAI GTCTC 6 cut(s) 24, 724, 1072, 1375, 1434, 1666
BsmFI GGGAC 1 cut(s) 695
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 3 cut(s) 833, 964, 2169
Bso31I GGTCTC 3 cut(s) 24, 1375, 1666
BsoBI CYCGRG 2 cut(s) 27, 768
Bsp119I TTCGAA 1 cut(s) 1822
Bsp1286I GDGCHC 3 cut(s) 92, 786, 871
Bsp143I GATC 7 cut(s) 179, 302, 337, 842, 1585, 1801, 2272
BspACI CCGC 4 cut(s) 748, 830, 1094, 1915
BspANI GGCC 3 cut(s) 833, 964, 2169
BspCNI CTCAG 3 cut(s) 104, 1134, 1245
BspLI GGNNCC 6 cut(s) 683, 785, 928, 1008, 2015, 2286
BspMAI CTGCAG 1 cut(s) 2206
BspPI GGATC 2 cut(s) 187, 850
BspQI GCTCTTC 3 cut(s) 80, 150, 1092
BspT104I TTCGAA 1 cut(s) 1822
BspT107I GGYRCC 1 cut(s) 2284
BspTNI GGTCTC 3 cut(s) 24, 1375, 1666
BsrDI GCAATG 1 cut(s) 1750
BsrFI RCCGGY 1 cut(s) 960
BsrI ACTGG 5 cut(s) 587, 1227, 1264, 2237, 2300
BssAI RCCGGY 1 cut(s) 960
BssECI CCNNGG 7 cut(s) 605, 1032, 1074, 1242, 1947, 2164, 2221
BssMI GATC 7 cut(s) 179, 302, 337, 842, 1585, 1801, 2272
BssNI GRCGYC 1 cut(s) 2285
BssSI CACGAG 3 cut(s) 788, 864, 1207
BssT1I CCWWGG 4 cut(s) 605, 1074, 1242, 2164
Bst2BI CACGAG 3 cut(s) 788, 864, 1207
Bst2UI CCWGG 3 cut(s) 924, 1033, 1949
Bst4CI ACNGT 6 cut(s) 455, 475, 562, 1891, 1922, 2151
Bst6I CTCTTC 7 cut(s) 80, 150, 293, 371, 588, 1092, 1437
BstACI GRCGYC 1 cut(s) 2285
BstBAI YACGTR 2 cut(s) 2098, 2131
BstBI TTCGAA 1 cut(s) 1822
BstC8I GCNNGC 7 cut(s) 6, 750, 762, 962, 2189, 2202, 2256
BstDEI CTNAG 5 cut(s) 91, 524, 1142, 1253, 1275
BstDSI CCRYGG 1 cut(s) 2221
BstEII GGTNACC 1 cut(s) 1594
BstH2I RGCGCY 2 cut(s) 1915, 2288
BstHHI GCGC 5 cut(s) 372, 1681, 1914, 2248, 2287
BstKTI GATC 7 cut(s) 182, 305, 340, 845, 1588, 1804, 2275
BstMAI GTCTC 6 cut(s) 24, 724, 1072, 1375, 1434, 1666
BstMBI GATC 7 cut(s) 179, 302, 337, 842, 1585, 1801, 2272
BstMWI GCNNNNNNNGC 9 cut(s) 150, 1091, 1232, 1277, 1653, 1771, 1964, 2166, 2197
BstNI CCWGG 3 cut(s) 924, 1033, 1949
BstNSI RCATGY 4 cut(s) 1468, 1778, 2191, 2215
BstPI GGTNACC 1 cut(s) 1594
BstSCI CCNGG 3 cut(s) 922, 1031, 1947
BstSFI CTRYAG 1 cut(s) 2202
BstV1I GCAGC 8 cut(s) 866, 931, 1219, 1222, 1283, 1631, 1783, 2172
BstV2I GAAGAC 3 cut(s) 660, 1870, 2214
BstX2I RGATCY 2 cut(s) 179, 337
BstYI RGATCY 2 cut(s) 179, 337
BsuRI GGCC 3 cut(s) 833, 964, 2169
BtgI CCRYGG 1 cut(s) 2221
BtgZI GCGATG 1 cut(s) 2201
BtsI GCAGTG 1 cut(s) 1107
BtsIMutI CAGTG 5 cut(s) 1107, 1234, 1896, 1918, 2244
Cac8I GCNNGC 7 cut(s) 6, 750, 762, 962, 2189, 2202, 2256
CfoI GCGC 5 cut(s) 372, 1681, 1914, 2248, 2287
Cfr10I RCCGGY 1 cut(s) 960
Cfr13I GGNCC 4 cut(s) 568, 682, 963, 1547
CseI GACGC 2 cut(s) 269, 1973
Csp6I GTAC 4 cut(s) 211, 471, 1199, 1778
CviAII CATG 8 cut(s) 430, 878, 1307, 1465, 1775, 1805, 2188, 2212
CviQI GTAC 4 cut(s) 211, 471, 1199, 1778
DdeI CTNAG 5 cut(s) 91, 524, 1142, 1253, 1275
DinI GGCGCC 1 cut(s) 2286
DpnI GATC 7 cut(s) 181, 304, 339, 844, 1587, 1803, 2274
DpnII GATC 7 cut(s) 179, 302, 337, 842, 1585, 1801, 2272
DraI TTTAAA 2 cut(s) 1981, 2112
Eam1104I CTCTTC 7 cut(s) 80, 150, 293, 371, 588, 1092, 1437
EarI CTCTTC 7 cut(s) 80, 150, 293, 371, 588, 1092, 1437
Ecl136II GAGCTC 1 cut(s) 90
Eco130I CCWWGG 4 cut(s) 605, 1074, 1242, 2164
Eco24I GRGCYC 2 cut(s) 92, 786
Eco31I GGTCTC 3 cut(s) 24, 1375, 1666
Eco47I GGWCC 3 cut(s) 568, 682, 1547
Eco53kI GAGCTC 1 cut(s) 90
Eco57I CTGAAG 3 cut(s) 552, 1338, 2022
Eco72I CACGTG 2 cut(s) 2098, 2131
Eco88I CYCGRG 2 cut(s) 27, 768
Eco91I GGTNACC 1 cut(s) 1594
EcoICRI GAGCTC 1 cut(s) 90
EcoO65I GGTNACC 1 cut(s) 1594
EcoRII CCWGG 3 cut(s) 922, 1031, 1947
EcoT14I CCWWGG 4 cut(s) 605, 1074, 1242, 2164
EcoT22I ATGCAT 2 cut(s) 1470, 2217
EcoT38I GRGCYC 2 cut(s) 92, 786
EgeI GGCGCC 1 cut(s) 2286
EheI GGCGCC 1 cut(s) 2286
ErhI CCWWGG 4 cut(s) 605, 1074, 1242, 2164
FaeI CATG 8 cut(s) 433, 881, 1310, 1468, 1778, 1808, 2191, 2215
FaqI GGGAC 1 cut(s) 695
FatI CATG 8 cut(s) 429, 877, 1306, 1464, 1774, 1804, 2187, 2211
FauI CCCGC 1 cut(s) 741
FbaI TGATCA 2 cut(s) 1801, 2272
FriOI GRGCYC 2 cut(s) 92, 786
FspBI CTAG 9 cut(s) 468, 606, 827, 957, 1091, 1107, 1176, 1472, 2192
GlaI GCGC 5 cut(s) 371, 1680, 1913, 2247, 2286
GsaI CCCAGC 1 cut(s) 1221
GsuI CTGGAG 2 cut(s) 30, 570
HaeII RGCGCY 2 cut(s) 1915, 2288
HaeIII GGCC 3 cut(s) 833, 964, 2169
HapII CCGG 2 cut(s) 961, 1512
HgaI GACGC 2 cut(s) 269, 1973
HhaI GCGC 5 cut(s) 372, 1681, 1914, 2248, 2287
Hin1I GRCGYC 1 cut(s) 2285
Hin1II CATG 8 cut(s) 433, 881, 1310, 1468, 1778, 1808, 2191, 2215
Hin6I GCGC 5 cut(s) 370, 1679, 1912, 2246, 2285
HinP1I GCGC 5 cut(s) 370, 1679, 1912, 2246, 2285
HincII GTYRAC 1 cut(s) 1189
HindII GTYRAC 1 cut(s) 1189
HindIII AAGCTT 1 cut(s) 1135
HinfI GANTC 4 cut(s) 1057, 1079, 1172, 1180
HpaI GTTAAC 1 cut(s) 1189
HpaII CCGG 2 cut(s) 961, 1512
HphI GGTGA 4 cut(s) 487, 916, 1259, 1472
Hpy166II GTNNAC 6 cut(s) 824, 1148, 1189, 1250, 2128, 2225
Hpy8I GTNNAC 6 cut(s) 824, 1148, 1189, 1250, 2128, 2225
HpyAV CCTTC 9 cut(s) 97, 169, 527, 705, 767, 943, 997, 1861, 2110
HpyCH4III ACNGT 6 cut(s) 455, 475, 562, 1891, 1922, 2151
HpyCH4IV ACGT 2 cut(s) 2097, 2130
HpyF10VI GCNNNNNNNGC 9 cut(s) 150, 1091, 1232, 1277, 1653, 1771, 1964, 2166, 2197
HpyF3I CTNAG 5 cut(s) 91, 524, 1142, 1253, 1275
HpySE526I ACGT 2 cut(s) 2097, 2130
Hsp92I GRCGYC 1 cut(s) 2285
Hsp92II CATG 8 cut(s) 433, 881, 1310, 1468, 1778, 1808, 2191, 2215
HspAI GCGC 5 cut(s) 370, 1679, 1912, 2246, 2285
KasI GGCGCC 1 cut(s) 2284
KroI GCCGGC 1 cut(s) 960
KroNI GCCGGC 1 cut(s) 962
Ksp22I TGATCA 2 cut(s) 1801, 2272
KspAI GTTAAC 1 cut(s) 1189
Kzo9I GATC 7 cut(s) 179, 302, 337, 842, 1585, 1801, 2272
LguI GCTCTTC 3 cut(s) 80, 150, 1092
LmnI GCTCC 5 cut(s) 789, 802, 1006, 1972, 2013
Lsp1109I GCAGC 8 cut(s) 866, 931, 1219, 1222, 1283, 1631, 1783, 2172
LweI GCATC 6 cut(s) 510, 1087, 1477, 1690, 1758, 2224
MaeI CTAG 9 cut(s) 468, 606, 827, 957, 1091, 1107, 1176, 1472, 2192
MaeII ACGT 2 cut(s) 2097, 2130
MaeIII GTNAC 2 cut(s) 1460, 1594
MalI GATC 7 cut(s) 181, 304, 339, 844, 1587, 1803, 2274
MboI GATC 7 cut(s) 179, 302, 337, 842, 1585, 1801, 2272
MfeI CAATTG 2 cut(s) 1695, 1989
MflI RGATCY 2 cut(s) 179, 337
MhlI GDGCHC 3 cut(s) 92, 786, 871
Mly113I GGCGCC 1 cut(s) 2285
MlyI GAGTC 2 cut(s) 1066, 1073
MmeI TCCRAC 4 cut(s) 492, 707, 954, 1012
Mph1103I ATGCAT 2 cut(s) 1470, 2217
MroNI GCCGGC 1 cut(s) 960
MroXI GAANNNNTTC 4 cut(s) 1152, 1323, 1705, 1818
MseI TTAA 7 cut(s) 614, 627, 735, 1188, 1980, 2111, 2262
MslI CAYNNNNRTG 2 cut(s) 876, 2220
MspI CCGG 2 cut(s) 961, 1512
MspR9I CCNGG 3 cut(s) 924, 1033, 1949
MunI CAATTG 2 cut(s) 1695, 1989
Mva1269I GAATGC 1 cut(s) 64
MvaI CCWGG 3 cut(s) 924, 1033, 1949
MwoI GCNNNNNNNGC 9 cut(s) 150, 1091, 1232, 1277, 1653, 1771, 1964, 2166, 2197
NaeI GCCGGC 1 cut(s) 962
NarI GGCGCC 1 cut(s) 2285
NdeII GATC 7 cut(s) 179, 302, 337, 842, 1585, 1801, 2272
NgoMIV GCCGGC 1 cut(s) 960
NlaIII CATG 8 cut(s) 433, 881, 1310, 1468, 1778, 1808, 2191, 2215
NlaIV GGNNCC 6 cut(s) 683, 785, 928, 1008, 2015, 2286
NmuCI GTSAC 1 cut(s) 1460
NsiI ATGCAT 2 cut(s) 1470, 2217
NspI RCATGY 4 cut(s) 1468, 1778, 2191, 2215
NspV TTCGAA 1 cut(s) 1822
PaeI GCATGC 1 cut(s) 2191
PaeR7I CTCGAG 2 cut(s) 27, 768
PciSI GCTCTTC 3 cut(s) 80, 150, 1092
PcsI WCGNNNNNNNCGW 1 cut(s) 2224
PctI GAATGC 1 cut(s) 64
PdiI GCCGGC 1 cut(s) 962
PdmI GAANNNNTTC 4 cut(s) 1152, 1323, 1705, 1818
PfeI GAWTC 2 cut(s) 1172, 1180
PleI GAGTC 2 cut(s) 1065, 1073
PluTI GGCGCC 1 cut(s) 2288
PmaCI CACGTG 2 cut(s) 2098, 2131
PmlI CACGTG 2 cut(s) 2098, 2131
PpsI GAGTC 2 cut(s) 1065, 1073
Ppu21I YACGTR 2 cut(s) 2098, 2131
PsiI TTATAA 1 cut(s) 2180
Psp124BI GAGCTC 1 cut(s) 92
Psp6I CCWGG 3 cut(s) 922, 1031, 1947
PspCI CACGTG 2 cut(s) 2098, 2131
PspEI GGTNACC 1 cut(s) 1594
PspFI CCCAGC 1 cut(s) 1217
PspGI CCWGG 3 cut(s) 922, 1031, 1947
PspN4I GGNNCC 6 cut(s) 683, 785, 928, 1008, 2015, 2286
PspPI GGNCC 4 cut(s) 568, 682, 963, 1547
PstI CTGCAG 1 cut(s) 2206
PsuI RGATCY 2 cut(s) 179, 337
RsaI GTAC 4 cut(s) 212, 472, 1200, 1779
RsaNI GTAC 4 cut(s) 211, 471, 1199, 1778
RseI CAYNNNNRTG 2 cut(s) 876, 2220
SacI GAGCTC 1 cut(s) 92
SapI GCTCTTC 3 cut(s) 80, 150, 1092
SaqAI TTAA 7 cut(s) 614, 627, 735, 1188, 1980, 2111, 2262
Sau3AI GATC 7 cut(s) 179, 302, 337, 842, 1585, 1801, 2272
Sau96I GGNCC 4 cut(s) 568, 682, 963, 1547
ScaI AGTACT 1 cut(s) 212
SchI GAGTC 2 cut(s) 1066, 1073
ScrFI CCNGG 3 cut(s) 924, 1033, 1949
SduI GDGCHC 3 cut(s) 92, 786, 871
SfaNI GCATC 6 cut(s) 510, 1087, 1477, 1690, 1758, 2224
SfcI CTRYAG 1 cut(s) 2202
SfoI GGCGCC 1 cut(s) 2286
Sfr274I CTCGAG 2 cut(s) 27, 768
SfuI TTCGAA 1 cut(s) 1822
SinI GGWCC 3 cut(s) 568, 682, 1547
SlaI CTCGAG 2 cut(s) 27, 768
SmiMI CAYNNNNRTG 2 cut(s) 876, 2220
SmlI CTYRAG 3 cut(s) 27, 161, 768
SmoI CTYRAG 3 cut(s) 27, 161, 768
SpeI ACTAGT 1 cut(s) 467
SphI GCATGC 1 cut(s) 2191
SsiI CCGC 4 cut(s) 748, 830, 1094, 1915
SspDI GGCGCC 1 cut(s) 2284
SspI AATATT 1 cut(s) 446
SspMI CTAG 9 cut(s) 468, 606, 827, 957, 1091, 1107, 1176, 1472, 2192
SstI GAGCTC 1 cut(s) 92
StyD4I CCNGG 3 cut(s) 922, 1031, 1947
StyI CCWWGG 4 cut(s) 605, 1074, 1242, 2164
TaaI ACNGT 6 cut(s) 455, 475, 562, 1891, 1922, 2151
TaiI ACGT 2 cut(s) 2100, 2133
TaqI TCGA 6 cut(s) 28, 305, 564, 769, 1203, 1822
TaqII GACCGA 2 cut(s) 279, 699
TatI WGTACW 2 cut(s) 210, 1198
TauI GCSGC 2 cut(s) 833, 1917
TfiI GAWTC 2 cut(s) 1172, 1180
Tru1I TTAA 7 cut(s) 614, 627, 735, 1188, 1980, 2111, 2262
Tru9I TTAA 7 cut(s) 614, 627, 735, 1188, 1980, 2111, 2262
TscAI CASTG 5 cut(s) 1107, 1234, 1896, 1925, 2244
TseFI GTSAC 1 cut(s) 1460
TseI GCWGC 8 cut(s) 854, 919, 1232, 1235, 1271, 1644, 1771, 2160
Tsp45I GTSAC 1 cut(s) 1460
TspRI CASTG 5 cut(s) 1107, 1234, 1896, 1925, 2244
VpaK11BI GGWCC 3 cut(s) 568, 682, 1547
XapI RAATTY 3 cut(s) 52, 1818, 1825
XceI RCATGY 4 cut(s) 1468, 1778, 2191, 2215
XcmI CCANNNNNNNNNTGG 1 cut(s) 2102
XhoI CTCGAG 2 cut(s) 27, 768
XmaJI CCTAGG 1 cut(s) 605
XmnI GAANNNNTTC 4 cut(s) 1152, 1323, 1705, 1818
XspI CTAG 9 cut(s) 468, 606, 827, 957, 1091, 1107, 1176, 1472, 2192
ZrmI AGTACT 1 cut(s) 212
Zsp2I ATGCAT 2 cut(s) 1470, 2217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.