Rh2CG339200

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001297
Physical Location & Seq
Reverse (-)
45134512 .. 45268398
133887 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG339200.1

Sequence Viewer

Length: 903 bp
ATGGTATCATCAAAACCAGCCTGCTTCGGCCCCTCTCGTCATCACTCAGGAGTCAGTTGTATTGAGGAATTCGTCACCGATGATTCAGTGATCGAAGTCCCGCAAAATTATGTTATCTCAGATCAGGAGTCTCCAGTCTCTGATGGGACTATCCTTGCCACAATCCCCACCATTGACATGAGACAGTTGGTCCTAATCAATCAGTCTGCAAACAACGATGAACTTGAGAAGTTGCACTCAACATGCAAAGAATGGGGAATTTTTCAGTTGGTTAACCATGGAGTTAGCTCTGCACTACTGGACAAAGTGAAGCAAGAATTAGAGGATTTTTTCAAGCTTCCCTTGGAAGAGAAAATGAGATACAAAGTAAGGCCAGGTGATGTTGAAGGCTATGGAACTGTGGTCAAATCCCAAGATCAAAAACTCGACTGGGGTAACAGGTTCTTCATGTTTACCAACCCGAGAAGGAAACCCCATCTTTTCCCGGAGCTCCCTACATCCCTGAGGAATACCTTGGAGACATACATGTTGGAATTACAAAGGATTGCCCGAACCCTTTTAGGGTTCATGGAAAAACTTCTAAAGATGAAGATGGAGGAGATGGAAGAGTTGTTTGAAGATGGTCTGCAGTCACTGAGGATGACTCACTATCCTCCATGCCCAAAACCAGAGCTAGTTGTTGGCCTCAGGCCTCATTCTGATGGTAGTGGCATCACTATTCTCCACCAGGTTGATGGAGTTCAAGGCCTTCAAATTAAAAAAGATGGGGCTTGGTTCCCTGTGATCTTTGACCATGGTGCCTTTGTTGTCAATGTTGGAGACATTCTAGAGGTTTGTGATGCCGTTCTCTTCACCCTCCATTTTCCTCTCCTCAAAAAGACACGTTGTCTATTAGTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

300

Amino Acids

34.07

Weight (kDa)

5.6

Isoelectric Point (pI)

48.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 55 - 158 3.3e-28 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 210 - 278 4.9e-18 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 797
AciI CCGC 1 cut(s) 101
AcsI RAATTY 2 cut(s) 68, 258
AfiI CCNNNNNNNGG 1 cut(s) 561
AflIII ACRYGT 2 cut(s) 525, 881
AgsI TTSAA 5 cut(s) 334, 386, 617, 743, 752
AhdI GACNNNNNGTC 2 cut(s) 188, 885
AjnI CCWGG 2 cut(s) 373, 726
AluBI AGCT 4 cut(s) 288, 337, 490, 673
AluI AGCT 4 cut(s) 288, 337, 490, 673
Alw21I GWGCWC 1 cut(s) 492
Alw26I GTCTC 5 cut(s) 135, 142, 175, 512, 813
AlwNI CAGNNNCTG 2 cut(s) 140, 634
Ama87I CYCGRG 1 cut(s) 460
AoxI GGCC 5 cut(s) 28, 371, 682, 689, 745
ApoI RAATTY 2 cut(s) 68, 258
Asp700I GAANNNNTTC 1 cut(s) 576
AspS9I GGNCC 2 cut(s) 29, 190
AsuC2I CCSGG 1 cut(s) 485
AsuHPI GGTGA 3 cut(s) 67, 389, 844
AvaI CYCGRG 1 cut(s) 460
AvaII GGWCC 1 cut(s) 190
AxyI CCTNAGG 2 cut(s) 503, 686
BanI GGYRCC 1 cut(s) 797
BanII GRGCYC 1 cut(s) 492
Bbv12I GWGCWC 1 cut(s) 492
BccI CCATC 8 cut(s) 137, 483, 586, 595, 614, 695, 728, 758
BceAI ACGGC 1 cut(s) 827
BciT130I CCWGG 2 cut(s) 375, 728
BcnI CCSGG 1 cut(s) 485
BcoDI GTCTC 5 cut(s) 135, 142, 175, 512, 813
BfaI CTAG 2 cut(s) 674, 827
BfmI CTRYAG 1 cut(s) 626
Bme1390I CCNGG 3 cut(s) 375, 485, 728
Bme18I GGWCC 1 cut(s) 190
BmeRI GACNNNNNGTC 2 cut(s) 188, 885
BmeT110I CYCGRG 1 cut(s) 460
BmgT120I GGNCC 2 cut(s) 29, 190
BmiI GGNNCC 3 cut(s) 31, 776, 799
BmrFI CCNGG 3 cut(s) 375, 485, 728
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 2 cut(s) 720, 829
BmuI ACTGGG 1 cut(s) 439
BplI GAGNNNNNCTC 2 cut(s) 628, 660
BpmI CTGGAG 1 cut(s) 117
BpuEI CTTGAG 1 cut(s) 245
BpuMI CCSGG 1 cut(s) 485
BsaJI CCNNGG 4 cut(s) 277, 342, 513, 793
Bsc4I CCNNNNNNNGG 1 cut(s) 561
Bse1I ACTGG 3 cut(s) 134, 303, 434
Bse21I CCTNAGG 2 cut(s) 503, 686
BseBI CCWGG 2 cut(s) 375, 728
BseDI CCNNGG 4 cut(s) 277, 342, 513, 793
BseGI GGATG 2 cut(s) 497, 645
BseLI CCNNNNNNNGG 1 cut(s) 561
BseMII CTCAG 5 cut(s) 60, 132, 494, 626, 700
BseNI ACTGG 3 cut(s) 134, 303, 434
BseRI GAGGAG 2 cut(s) 611, 860
BsgI GTGCAG 1 cut(s) 276
BshFI GGCC 5 cut(s) 30, 373, 684, 691, 747
BshNI GGYRCC 1 cut(s) 797
BsiHKAI GWGCWC 1 cut(s) 492
BsiHKCI CYCGRG 1 cut(s) 460
BsiSI CCGG 1 cut(s) 485
BslFI GGGAC 2 cut(s) 83, 160
BslI CCNNNNNNNGG 1 cut(s) 561
BsmAI GTCTC 5 cut(s) 135, 142, 175, 512, 813
BsmFI GGGAC 2 cut(s) 83, 160
BsnI GGCC 5 cut(s) 30, 373, 684, 691, 747
BsoBI CYCGRG 1 cut(s) 460
Bsp1286I GDGCHC 1 cut(s) 492
Bsp143I GATC 4 cut(s) 90, 121, 415, 783
Bsp19I CCATGG 2 cut(s) 277, 793
BspACI CCGC 1 cut(s) 101
BspANI GGCC 5 cut(s) 30, 373, 684, 691, 747
BspCNI CTCAG 5 cut(s) 59, 131, 495, 627, 699
BspLI GGNNCC 3 cut(s) 31, 776, 799
BspMAI CTGCAG 1 cut(s) 630
BspT107I GGYRCC 1 cut(s) 797
BsrI ACTGG 3 cut(s) 134, 303, 434
BssECI CCNNGG 4 cut(s) 277, 342, 513, 793
BssMI GATC 4 cut(s) 90, 121, 415, 783
BssT1I CCWWGG 4 cut(s) 277, 342, 513, 793
Bst2UI CCWGG 2 cut(s) 375, 728
Bst4CI ACNGT 2 cut(s) 186, 400
Bst6I CTCTTC 3 cut(s) 342, 600, 854
BstC8I GCNNGC 1 cut(s) 22
BstDEI CTNAG 5 cut(s) 46, 118, 503, 635, 686
BstDSI CCRYGG 2 cut(s) 277, 793
BstF5I GGATG 2 cut(s) 497, 645
BstKTI GATC 4 cut(s) 93, 124, 418, 786
BstMAI GTCTC 5 cut(s) 135, 142, 175, 512, 813
BstMBI GATC 4 cut(s) 90, 121, 415, 783
BstNI CCWGG 2 cut(s) 375, 728
BstNSI RCATGY 2 cut(s) 246, 529
BstSCI CCNGG 3 cut(s) 373, 483, 726
BstSFI CTRYAG 1 cut(s) 626
BstXI CCANNNNNNTGG 1 cut(s) 734
Bsu36I CCTNAGG 2 cut(s) 503, 686
BsuRI GGCC 5 cut(s) 30, 373, 684, 691, 747
BtgI CCRYGG 2 cut(s) 277, 793
BtsCI GGATG 2 cut(s) 497, 645
BtsIMutI CAGTG 2 cut(s) 93, 632
Cac8I GCNNGC 1 cut(s) 22
CaiI CAGNNNCTG 2 cut(s) 140, 634
Cfr13I GGNCC 2 cut(s) 29, 190
CsiI ACCWGGT 1 cut(s) 726
CviAII CATG 8 cut(s) 178, 243, 278, 448, 526, 568, 657, 794
DdeI CTNAG 5 cut(s) 46, 118, 503, 635, 686
DpnI GATC 4 cut(s) 92, 123, 417, 785
DpnII GATC 4 cut(s) 90, 121, 415, 783
DriI GACNNNNNGTC 2 cut(s) 188, 885
Eam1104I CTCTTC 3 cut(s) 342, 600, 854
Eam1105I GACNNNNNGTC 2 cut(s) 188, 885
EarI CTCTTC 3 cut(s) 342, 600, 854
Ecl136II GAGCTC 1 cut(s) 490
Eco130I CCWWGG 4 cut(s) 277, 342, 513, 793
Eco147I AGGCCT 2 cut(s) 691, 747
Eco24I GRGCYC 1 cut(s) 492
Eco47I GGWCC 1 cut(s) 190
Eco53kI GAGCTC 1 cut(s) 490
Eco81I CCTNAGG 2 cut(s) 503, 686
Eco88I CYCGRG 1 cut(s) 460
EcoICRI GAGCTC 1 cut(s) 490
EcoRI GAATTC 1 cut(s) 68
EcoRII CCWGG 2 cut(s) 373, 726
EcoT14I CCWWGG 4 cut(s) 277, 342, 513, 793
EcoT38I GRGCYC 1 cut(s) 492
ErhI CCWWGG 4 cut(s) 277, 342, 513, 793
FaeI CATG 8 cut(s) 181, 246, 281, 451, 529, 571, 660, 797
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FaqI GGGAC 2 cut(s) 83, 160
FatI CATG 8 cut(s) 177, 242, 277, 447, 525, 567, 656, 793
FauI CCCGC 1 cut(s) 108
FokI GGATG 2 cut(s) 484, 652
FriOI GRGCYC 1 cut(s) 492
FspBI CTAG 2 cut(s) 674, 827
GsuI CTGGAG 1 cut(s) 117
HaeIII GGCC 5 cut(s) 30, 373, 684, 691, 747
HapII CCGG 1 cut(s) 485
Hin1II CATG 8 cut(s) 181, 246, 281, 451, 529, 571, 660, 797
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 4 cut(s) 51, 83, 128, 643
HpaI GTTAAC 1 cut(s) 274
HpaII CCGG 1 cut(s) 485
HphI GGTGA 3 cut(s) 67, 389, 844
Hpy166II GTNNAC 2 cut(s) 274, 453
Hpy188I TCNGA 3 cut(s) 121, 142, 700
Hpy188III TCNNGA 3 cut(s) 48, 125, 827
Hpy8I GTNNAC 2 cut(s) 274, 453
HpyAV CCTTC 3 cut(s) 380, 459, 758
HpyCH4III ACNGT 2 cut(s) 186, 400
HpyCH4IV ACGT 1 cut(s) 883
HpyCH4V TGCA 5 cut(s) 209, 235, 246, 293, 628
HpyF3I CTNAG 5 cut(s) 46, 118, 503, 635, 686
HpySE526I ACGT 1 cut(s) 883
Hsp92II CATG 8 cut(s) 181, 246, 281, 451, 529, 571, 660, 797
KspAI GTTAAC 1 cut(s) 274
Kzo9I GATC 4 cut(s) 90, 121, 415, 783
LmnI GCTCC 2 cut(s) 487, 495
LweI GCATC 2 cut(s) 720, 829
MabI ACCWGGT 1 cut(s) 726
MaeI CTAG 2 cut(s) 674, 827
MaeII ACGT 1 cut(s) 883
MaeIII GTNAC 3 cut(s) 73, 434, 630
MalI GATC 4 cut(s) 92, 123, 417, 785
MboI GATC 4 cut(s) 90, 121, 415, 783
MboII GAAGA 6 cut(s) 359, 436, 601, 617, 629, 841
MhlI GDGCHC 1 cut(s) 492
MluCI AATT 6 cut(s) 68, 106, 258, 317, 533, 753
MlyI GAGTC 3 cut(s) 60, 137, 637
MmeI TCCRAC 2 cut(s) 510, 796
MroXI GAANNNNTTC 1 cut(s) 576
MseI TTAA 2 cut(s) 273, 756
MslI CAYNNNNRTG 2 cut(s) 176, 699
MspI CCGG 1 cut(s) 485
MspR9I CCNGG 3 cut(s) 375, 485, 728
MvaI CCWGG 2 cut(s) 375, 728
NciI CCSGG 1 cut(s) 485
NcoI CCATGG 2 cut(s) 277, 793
NdeII GATC 4 cut(s) 90, 121, 415, 783
NlaIII CATG 8 cut(s) 181, 246, 281, 451, 529, 571, 660, 797
NlaIV GGNNCC 3 cut(s) 31, 776, 799
NmuCI GTSAC 2 cut(s) 73, 630
NspI RCATGY 2 cut(s) 246, 529
PceI AGGCCT 2 cut(s) 691, 747
PciI ACATGT 1 cut(s) 525
PdmI GAANNNNTTC 1 cut(s) 576
PfeI GAWTC 1 cut(s) 83
PfoI TCCNGGA 1 cut(s) 483
PleI GAGTC 3 cut(s) 59, 136, 637
PpsI GAGTC 3 cut(s) 59, 136, 637
PscI ACATGT 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 492
Psp6I CCWGG 2 cut(s) 373, 726
PspGI CCWGG 2 cut(s) 373, 726
PspN4I GGNNCC 3 cut(s) 31, 776, 799
PspPI GGNCC 2 cut(s) 29, 190
PstI CTGCAG 1 cut(s) 630
PstNI CAGNNNCTG 2 cut(s) 140, 634
RseI CAYNNNNRTG 2 cut(s) 176, 699
SacI GAGCTC 1 cut(s) 492
SaqAI TTAA 2 cut(s) 273, 756
Sau3AI GATC 4 cut(s) 90, 121, 415, 783
Sau96I GGNCC 2 cut(s) 29, 190
SchI GAGTC 3 cut(s) 60, 137, 637
ScrFI CCNGG 3 cut(s) 375, 485, 728
SduI GDGCHC 1 cut(s) 492
SexAI ACCWGGT 1 cut(s) 726
SfaNI GCATC 2 cut(s) 720, 829
SfcI CTRYAG 1 cut(s) 626
SinI GGWCC 1 cut(s) 190
SmiMI CAYNNNNRTG 2 cut(s) 176, 699
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 6 cut(s) 68, 106, 258, 317, 533, 753
SseBI AGGCCT 2 cut(s) 691, 747
SsiI CCGC 1 cut(s) 101
SspMI CTAG 2 cut(s) 674, 827
SstI GAGCTC 1 cut(s) 492
StuI AGGCCT 2 cut(s) 691, 747
StyD4I CCNGG 3 cut(s) 373, 483, 726
StyI CCWWGG 4 cut(s) 277, 342, 513, 793
TaaI ACNGT 2 cut(s) 186, 400
TaiI ACGT 1 cut(s) 886
TaqI TCGA 2 cut(s) 93, 426
TasI AATT 6 cut(s) 68, 106, 258, 317, 533, 753
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 2 cut(s) 273, 756
Tru9I TTAA 2 cut(s) 273, 756
TscAI CASTG 2 cut(s) 93, 639
TseFI GTSAC 2 cut(s) 73, 630
Tsp45I GTSAC 2 cut(s) 73, 630
TspDTI ATGAA 4 cut(s) 234, 436, 556, 602
TspRI CASTG 2 cut(s) 93, 639
VpaK11BI GGWCC 1 cut(s) 190
XapI RAATTY 2 cut(s) 68, 258
XbaI TCTAGA 1 cut(s) 826
XceI RCATGY 2 cut(s) 246, 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 731
XmnI GAANNNNTTC 1 cut(s) 576
XspI CTAG 2 cut(s) 674, 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.