Rh2DG378800

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001297
Physical Location & Seq
Reverse (-)
54389975 .. 54393041
3067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG378800.1

Sequence Viewer

Length: 1104 bp
ATGGTATCATCAAAACCAGGAAGCAGCTTTGGCCCCTCTCATCATGACTCAGGAGTCAGTAGTATTGAGAAACTCATCACCGATGATTCAGTGATCGAAGTCCCCCAAAACTATGTTATCTCAGATCAGGAGTCTCCGGTCTCTGATGGGACTATCCTTGCCACAATCCCCACCATTGATATGAGACAGTTGGTCCTAATCAATCAGTCTGCAAACAAAGATGAACTTGAAAAGTTGCACTCAACTTGCAAAGAATGGGGAATTTTTCAGTTGGTTAACCATGGAGTTAGCTCTGCACTACTGGACAAAGTGAAGCAAGATTTAGAGGCATTTTTCAAGCTTCCCTTGGAAGAGAAAATGAGATACAAAGTAAGGCCAGGTGATGTTGAAGGGTATGGAACTGTTGTCAAATCCCAAGATCAAAAACTCGACTGGGGTAACAGGTTGTACATGTTTACCAACCCTAGAAGGAAACCCCATCTTTTCCCAGAGCTCCCTACATCCCTGAGGAATACCTTGGAGTGGTACATGTTGGAATTACAAAGGATTGCCCGGACCCTTTTAGGGTTCATGGGAAAACTTCTAAAGATGAAGATGGAGGAGATGGAAGAGTTGTTTCAAGATGGGCTGCAGTCACTGAGGATGACTCACTATCCTCCATGCCCACAACCAGGGCTAGTTGGTGGCCTCAGGCCTCATTCTGATGGTAGTGGCATCACTATTCTCCACCAGGTTAATGGAGTTCAAGGCCTTCAAATTAAAAAAGATGGGGCTTGGTTCCCTGTGATCTTTGACCATGGTGCCTTTGTTGTCAATGTTGGGGACATTCTAGAGATTCTGAGTGATGGGCTTTATAAAAGTATTGAGCACAGAGTAATTGTGAACCCAGAGAAAGAAAGGATTTCAATTGCTATGTTCTTCATGCCCAAACTTGAGTCAGAAATTGGACCTGTGACCGCCCTGAGAGATCCTCAGAAGCCACCATCTCCTGGGAAAATAGGAATGGCAAAATTTGTCGAGAATTACTTCGCTCATAATCTTAATGGAAAAACACCCTTGGAGCGAATCAGAACAAAAGATGGTGAAGAGAATACTACTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

367

Amino Acids

41.26

Weight (kDa)

6.15

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 56 - 159 5.9e-28 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 211 - 309 2.9e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 855
AasI GACNNNNNNGTC 1 cut(s) 53
AccB1I GGYRCC 1 cut(s) 800
AccB7I CCANNNNNTGG 1 cut(s) 989
AciI CCGC 1 cut(s) 957
AclWI GGATC 1 cut(s) 962
AcsI RAATTY 2 cut(s) 261, 1010
AfaI GTAC 2 cut(s) 449, 527
AfiI CCNNNNNNNGG 4 cut(s) 522, 564, 671, 989
AflIII ACRYGT 2 cut(s) 450, 528
AgsI TTSAA 7 cut(s) 230, 337, 389, 620, 746, 755, 906
AhdI GACNNNNNGTC 1 cut(s) 191
AjnI CCWGG 5 cut(s) 16, 376, 670, 729, 988
AluBI AGCT 4 cut(s) 27, 291, 340, 493
AluI AGCT 4 cut(s) 27, 291, 340, 493
Alw21I GWGCWC 2 cut(s) 495, 870
Alw26I GTCTC 3 cut(s) 138, 145, 178
AlwI GGATC 1 cut(s) 962
AlwNI CAGNNNCTG 1 cut(s) 637
AoxI GGCC 5 cut(s) 31, 374, 685, 692, 748
ApeKI GCWGC 2 cut(s) 24, 628
ApoI RAATTY 2 cut(s) 261, 1010
Asp700I GAANNNNTTC 1 cut(s) 1025
AspS9I GGNCC 4 cut(s) 32, 193, 555, 947
AsuC2I CCSGG 1 cut(s) 553
AsuHPI GGTGA 3 cut(s) 70, 392, 1094
AvaII GGWCC 3 cut(s) 193, 555, 947
AxyI CCTNAGG 2 cut(s) 506, 689
BanI GGYRCC 1 cut(s) 800
BanII GRGCYC 1 cut(s) 495
Bbv12I GWGCWC 2 cut(s) 495, 870
BbvI GCAGC 2 cut(s) 36, 615
BciT130I CCWGG 5 cut(s) 18, 378, 672, 731, 990
BcnI CCSGG 1 cut(s) 553
BcoDI GTCTC 3 cut(s) 138, 145, 178
BfaI CTAG 3 cut(s) 465, 677, 830
BfmI CTRYAG 1 cut(s) 629
BisI GCNGC 2 cut(s) 25, 629
BlsI GCNGC 2 cut(s) 26, 630
Bme1390I CCNGG 6 cut(s) 18, 378, 553, 672, 731, 990
Bme18I GGWCC 3 cut(s) 193, 555, 947
BmeRI GACNNNNNGTC 1 cut(s) 191
BmgT120I GGNCC 4 cut(s) 32, 193, 555, 947
BmiI GGNNCC 4 cut(s) 34, 557, 779, 802
BmrFI CCNGG 6 cut(s) 18, 378, 553, 672, 731, 990
BmrI ACTGGG 1 cut(s) 442
BmsI GCATC 1 cut(s) 723
BmuI ACTGGG 1 cut(s) 442
BplI GAGNNNNNCTC 4 cut(s) 631, 663, 955, 987
BpuEI CTTGAG 1 cut(s) 953
BpuMI CCSGG 1 cut(s) 553
BsaI GGTCTC 1 cut(s) 145
BsaJI CCNNGG 7 cut(s) 280, 345, 516, 671, 796, 989, 1056
BsaWI WCCGGW 1 cut(s) 136
Bsc4I CCNNNNNNNGG 4 cut(s) 522, 564, 671, 989
Bse1I ACTGG 2 cut(s) 306, 437
Bse21I CCTNAGG 2 cut(s) 506, 689
BseBI CCWGG 5 cut(s) 18, 378, 672, 731, 990
BseDI CCNNGG 7 cut(s) 280, 345, 516, 671, 796, 989, 1056
BseGI GGATG 2 cut(s) 500, 648
BseLI CCNNNNNNNGG 4 cut(s) 522, 564, 671, 989
BseMII CTCAG 8 cut(s) 63, 135, 497, 629, 703, 830, 953, 986
BseNI ACTGG 2 cut(s) 306, 437
BseRI GAGGAG 1 cut(s) 614
BseXI GCAGC 2 cut(s) 36, 615
BsgI GTGCAG 1 cut(s) 279
BshFI GGCC 5 cut(s) 33, 376, 687, 694, 750
BshNI GGYRCC 1 cut(s) 800
BsiHKAI GWGCWC 2 cut(s) 495, 870
BsiSI CCGG 2 cut(s) 137, 553
BslFI GGGAC 3 cut(s) 86, 163, 836
BslI CCNNNNNNNGG 4 cut(s) 522, 564, 671, 989
BsmAI GTCTC 3 cut(s) 138, 145, 178
BsmFI GGGAC 3 cut(s) 86, 163, 836
BsnI GGCC 5 cut(s) 33, 376, 687, 694, 750
Bso31I GGTCTC 1 cut(s) 145
Bsp1286I GDGCHC 2 cut(s) 495, 870
Bsp1407I TGTACA 1 cut(s) 447
Bsp143I GATC 5 cut(s) 93, 124, 418, 786, 967
Bsp19I CCATGG 2 cut(s) 280, 796
BspACI CCGC 1 cut(s) 957
BspANI GGCC 5 cut(s) 33, 376, 687, 694, 750
BspCNI CTCAG 8 cut(s) 62, 134, 498, 630, 702, 831, 954, 985
BspHI TCATGA 1 cut(s) 43
BspLI GGNNCC 4 cut(s) 34, 557, 779, 802
BspMAI CTGCAG 1 cut(s) 633
BspPI GGATC 1 cut(s) 962
BspT107I GGYRCC 1 cut(s) 800
BspTNI GGTCTC 1 cut(s) 145
BsrGI TGTACA 1 cut(s) 447
BsrI ACTGG 2 cut(s) 306, 437
BssECI CCNNGG 7 cut(s) 280, 345, 516, 671, 796, 989, 1056
BssMI GATC 5 cut(s) 93, 124, 418, 786, 967
BssT1I CCWWGG 5 cut(s) 280, 345, 516, 796, 1056
Bst2UI CCWGG 5 cut(s) 18, 378, 672, 731, 990
Bst4CI ACNGT 2 cut(s) 189, 403
Bst6I CTCTTC 3 cut(s) 345, 603, 1080
BstAUI TGTACA 1 cut(s) 447
BstDEI CTNAG 8 cut(s) 49, 121, 506, 638, 689, 839, 962, 972
BstDSI CCRYGG 2 cut(s) 280, 796
BstF5I GGATG 2 cut(s) 500, 648
BstKTI GATC 5 cut(s) 96, 127, 421, 789, 970
BstMAI GTCTC 3 cut(s) 138, 145, 178
BstMBI GATC 5 cut(s) 93, 124, 418, 786, 967
BstMWI GCNNNNNNNGC 1 cut(s) 30
BstNI CCWGG 5 cut(s) 18, 378, 672, 731, 990
BstNSI RCATGY 2 cut(s) 454, 532
BstSCI CCNGG 6 cut(s) 16, 376, 551, 670, 729, 988
BstSFI CTRYAG 1 cut(s) 629
BstV1I GCAGC 2 cut(s) 36, 615
BstX2I RGATCY 1 cut(s) 967
BstXI CCANNNNNNTGG 1 cut(s) 737
BstYI RGATCY 1 cut(s) 967
Bsu36I CCTNAGG 2 cut(s) 506, 689
BsuRI GGCC 5 cut(s) 33, 376, 687, 694, 750
BtgI CCRYGG 2 cut(s) 280, 796
BtsCI GGATG 2 cut(s) 500, 648
BtsIMutI CAGTG 2 cut(s) 96, 635
CaiI CAGNNNCTG 1 cut(s) 637
CciI TCATGA 1 cut(s) 43
Cfr13I GGNCC 4 cut(s) 32, 193, 555, 947
CsiI ACCWGGT 1 cut(s) 729
Csp6I GTAC 2 cut(s) 448, 526
CviAII CATG 9 cut(s) 44, 281, 451, 529, 571, 660, 797, 922, 1101
CviQI GTAC 2 cut(s) 448, 526
DdeI CTNAG 8 cut(s) 49, 121, 506, 638, 689, 839, 962, 972
DpnI GATC 5 cut(s) 95, 126, 420, 788, 969
DpnII GATC 5 cut(s) 93, 124, 418, 786, 967
DrdI GACNNNNNNGTC 1 cut(s) 53
DriI GACNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 53
Eam1104I CTCTTC 3 cut(s) 345, 603, 1080
Eam1105I GACNNNNNGTC 1 cut(s) 191
EarI CTCTTC 3 cut(s) 345, 603, 1080
Ecl136II GAGCTC 1 cut(s) 493
Eco130I CCWWGG 5 cut(s) 280, 345, 516, 796, 1056
Eco147I AGGCCT 2 cut(s) 694, 750
Eco24I GRGCYC 1 cut(s) 495
Eco31I GGTCTC 1 cut(s) 145
Eco47I GGWCC 3 cut(s) 193, 555, 947
Eco53kI GAGCTC 1 cut(s) 493
Eco81I CCTNAGG 2 cut(s) 506, 689
EcoICRI GAGCTC 1 cut(s) 493
EcoRII CCWGG 5 cut(s) 16, 376, 670, 729, 988
EcoT14I CCWWGG 5 cut(s) 280, 345, 516, 796, 1056
EcoT38I GRGCYC 1 cut(s) 495
ErhI CCWWGG 5 cut(s) 280, 345, 516, 796, 1056
FaeI CATG 9 cut(s) 47, 284, 454, 532, 574, 663, 800, 925, 1104
FalI AAGNNNNNCTT 4 cut(s) 210, 242, 329, 361
FaqI GGGAC 3 cut(s) 86, 163, 836
FatI CATG 9 cut(s) 43, 280, 450, 528, 570, 659, 796, 921, 1100
Fnu4HI GCNGC 2 cut(s) 25, 629
FokI GGATG 2 cut(s) 487, 655
FriOI GRGCYC 1 cut(s) 495
Fsp4HI GCNGC 2 cut(s) 25, 629
FspBI CTAG 3 cut(s) 465, 677, 830
GluI GCNGC 2 cut(s) 25, 629
HaeIII GGCC 5 cut(s) 33, 376, 687, 694, 750
HapII CCGG 2 cut(s) 137, 553
Hin1II CATG 9 cut(s) 47, 284, 454, 532, 574, 663, 800, 925, 1104
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 8 cut(s) 47, 54, 86, 131, 646, 835, 935, 1065
HpaI GTTAAC 1 cut(s) 277
HpaII CCGG 2 cut(s) 137, 553
HphI GGTGA 3 cut(s) 70, 392, 1094
Hpy166II GTNNAC 3 cut(s) 277, 456, 883
Hpy188I TCNGA 7 cut(s) 124, 145, 703, 840, 940, 975, 1070
Hpy188III TCNNGA 6 cut(s) 44, 51, 128, 620, 830, 1018
Hpy8I GTNNAC 3 cut(s) 277, 456, 883
HpyAV CCTTC 3 cut(s) 383, 462, 761
HpyCH4III ACNGT 2 cut(s) 189, 403
HpyCH4V TGCA 6 cut(s) 212, 238, 249, 296, 631, 1100
HpyF10VI GCNNNNNNNGC 1 cut(s) 30
HpyF3I CTNAG 8 cut(s) 49, 121, 506, 638, 689, 839, 962, 972
Hsp92II CATG 9 cut(s) 47, 284, 454, 532, 574, 663, 800, 925, 1104
KspAI GTTAAC 1 cut(s) 277
Kzo9I GATC 5 cut(s) 93, 124, 418, 786, 967
LmnI GCTCC 2 cut(s) 498, 1060
Lsp1109I GCAGC 2 cut(s) 36, 615
LweI GCATC 1 cut(s) 723
MabI ACCWGGT 1 cut(s) 729
MaeI CTAG 3 cut(s) 465, 677, 830
MaeIII GTNAC 3 cut(s) 437, 633, 952
MalI GATC 5 cut(s) 95, 126, 420, 788, 969
MboI GATC 5 cut(s) 93, 124, 418, 786, 967
MboII GAAGA 5 cut(s) 362, 604, 620, 910, 1097
MfeI CAATTG 1 cut(s) 906
MflI RGATCY 1 cut(s) 967
MhlI GDGCHC 2 cut(s) 495, 870
MluCI AATT 8 cut(s) 261, 536, 756, 876, 906, 942, 1010, 1021
MlyI GAGTC 5 cut(s) 41, 63, 140, 640, 944
MmeI TCCRAC 1 cut(s) 513
MnlI CCTC 9 cut(s) 46, 319, 501, 592, 633, 666, 698, 705, 981
MroXI GAANNNNTTC 1 cut(s) 1025
MseI TTAA 4 cut(s) 276, 735, 759, 1041
MslI CAYNNNNRTG 2 cut(s) 179, 702
MspI CCGG 2 cut(s) 137, 553
MspR9I CCNGG 6 cut(s) 18, 378, 553, 672, 731, 990
MunI CAATTG 1 cut(s) 906
MvaI CCWGG 5 cut(s) 18, 378, 672, 731, 990
MwoI GCNNNNNNNGC 1 cut(s) 30
NciI CCSGG 1 cut(s) 553
NcoI CCATGG 2 cut(s) 280, 796
NdeII GATC 5 cut(s) 93, 124, 418, 786, 967
NlaIII CATG 9 cut(s) 47, 284, 454, 532, 574, 663, 800, 925, 1104
NlaIV GGNNCC 4 cut(s) 34, 557, 779, 802
NmuCI GTSAC 2 cut(s) 633, 952
NspI RCATGY 2 cut(s) 454, 532
PagI TCATGA 1 cut(s) 43
PceI AGGCCT 2 cut(s) 694, 750
PciI ACATGT 2 cut(s) 450, 528
PdmI GAANNNNTTC 1 cut(s) 1025
PfeI GAWTC 3 cut(s) 86, 835, 1065
PflMI CCANNNNNTGG 1 cut(s) 989
PkrI GCNGC 2 cut(s) 26, 630
PleI GAGTC 5 cut(s) 41, 62, 139, 640, 943
PpsI GAGTC 5 cut(s) 41, 62, 139, 640, 943
PscI ACATGT 2 cut(s) 450, 528
PsiI TTATAA 1 cut(s) 855
Psp124BI GAGCTC 1 cut(s) 495
Psp6I CCWGG 5 cut(s) 16, 376, 670, 729, 988
PspGI CCWGG 5 cut(s) 16, 376, 670, 729, 988
PspN4I GGNNCC 4 cut(s) 34, 557, 779, 802
PspPI GGNCC 4 cut(s) 32, 193, 555, 947
PstI CTGCAG 1 cut(s) 633
PstNI CAGNNNCTG 1 cut(s) 637
PsuI RGATCY 1 cut(s) 967
RsaI GTAC 2 cut(s) 449, 527
RsaNI GTAC 2 cut(s) 448, 526
RseI CAYNNNNRTG 2 cut(s) 179, 702
SacI GAGCTC 1 cut(s) 495
SaqAI TTAA 4 cut(s) 276, 735, 759, 1041
SatI GCNGC 2 cut(s) 25, 629
Sau3AI GATC 5 cut(s) 93, 124, 418, 786, 967
Sau96I GGNCC 4 cut(s) 32, 193, 555, 947
SchI GAGTC 5 cut(s) 41, 63, 140, 640, 944
ScrFI CCNGG 6 cut(s) 18, 378, 553, 672, 731, 990
SduI GDGCHC 2 cut(s) 495, 870
SetI ASST 9 cut(s) 29, 293, 342, 382, 446, 495, 518, 735, 952
SexAI ACCWGGT 1 cut(s) 729
SfaNI GCATC 1 cut(s) 723
SfcI CTRYAG 1 cut(s) 629
SinI GGWCC 3 cut(s) 193, 555, 947
SmiMI CAYNNNNRTG 2 cut(s) 179, 702
SmlI CTYRAG 1 cut(s) 932
SmoI CTYRAG 1 cut(s) 932
Sse9I AATT 8 cut(s) 261, 536, 756, 876, 906, 942, 1010, 1021
SseBI AGGCCT 2 cut(s) 694, 750
SsiI CCGC 1 cut(s) 957
SspMI CTAG 3 cut(s) 465, 677, 830
SstI GAGCTC 1 cut(s) 495
StuI AGGCCT 2 cut(s) 694, 750
StyD4I CCNGG 6 cut(s) 16, 376, 551, 670, 729, 988
StyI CCWWGG 5 cut(s) 280, 345, 516, 796, 1056
TaaI ACNGT 2 cut(s) 189, 403
TaqI TCGA 3 cut(s) 96, 429, 1017
TasI AATT 8 cut(s) 261, 536, 756, 876, 906, 942, 1010, 1021
TatI WGTACW 1 cut(s) 447
TfiI GAWTC 3 cut(s) 86, 835, 1065
Tru1I TTAA 4 cut(s) 276, 735, 759, 1041
Tru9I TTAA 4 cut(s) 276, 735, 759, 1041
TscAI CASTG 2 cut(s) 96, 642
TseFI GTSAC 2 cut(s) 633, 952
TseI GCWGC 2 cut(s) 24, 628
Tsp45I GTSAC 2 cut(s) 633, 952
TspDTI ATGAA 4 cut(s) 237, 559, 605, 910
TspRI CASTG 2 cut(s) 96, 642
Van91I CCANNNNNTGG 1 cut(s) 989
VpaK11BI GGWCC 3 cut(s) 193, 555, 947
XapI RAATTY 2 cut(s) 261, 1010
XbaI TCTAGA 1 cut(s) 829
XceI RCATGY 2 cut(s) 454, 532
XcmI CCANNNNNNNNNTGG 1 cut(s) 734
XmnI GAANNNNTTC 1 cut(s) 1025
XspI CTAG 3 cut(s) 465, 677, 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.