RLG00000031950

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001297
Physical Location & Seq
Forward (+)
9765705 .. 9773510
7806 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031950

Sequence Viewer

Length: 1107 bp
ATGGCATCACCCCAGCTTTGGCTACCAGGAGGCTTTGAGAGCTCTAGTTCTCTTATGAGCATCCTGGAGATCACCCAAGAGTCATTAACTGAAGTCCCACAATGTTACGTCTGTAGTTTCGAAGAACCAGAACTTTCATCTGATCTCTCTGATAAGCCCACCGCAATCCCAACCATCGATATGATAAAACTAGTAATGGGATCAGAAGCTTCAGATTCTGAACTGGAAAATCTGCGCTCTACTTGCAAAGAGTGGGGAATCTTTCAGTTGGCGAACCATGGAGTTAGCCCTATGCTTTTGGAGAAGCTGAAGTACGAGGTTGAAGAATTTTTTAAGCGTCCCTTGGAAGAGAAAATGAGCTATGGATCCCTAGCCCAAAATAAAGAGCAAAAACGTGACTGGGGTAATAGATTCTACATGACCATGAACCCTGTTCATAGGAGAAAGCCATACCTCTTCCCACAGCTCCCTTCATCCCTCAGGAGTACCTTCGAGTCATACTACTTGGAACTGAAGCAACTAGCCATGGAACTTCTTGGATTTCTGGGGAAGGCTGTGAAGATAGACATAAAAGAGATTGAGGAGATGTTTGAAGATGGGATGCAGTCAGTGAGGATGAATTACTATCCTGCATGCCCACAACCAGAGGTGGTCATCGGCCTCACACCTCACTCGGACGCTTCCGGGATCACCATCCTCCACCAGCTTAACGGTGTGGGTGGTCTGCAGATTAAGAAAGATGGGGTTTGGGTTCCTGTCAACTTCCGTGAAGATGCCTTTGTGGTTAATGCGGGGGACATCTTTGAGCTTGCCGTCGCGGTACTCGAAGAGGAAGAGCAACAGAGCAGTATACCAAATTATGCACTGGAGCACGATGACCTGGACCATAAGGCTGCCGGACTCCGGGATTTGGTTGCTATGACTGTCAATGATATTGTCACATCTGGAGCCAAGCCATTATTTTTCCTCGATTACTTTGCTACAAGCTACCTTGATGTTGATCTTGCTGAAAAGGAGTTCTGTAGATTCATGAAAGAACATGGATGGTTGGATGGAAAGGTTGACGCTTTGAAGTATCTTGTTTCACACTTCCAACTTTACTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

369

Amino Acids

41.86

Weight (kDa)

4.72

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 56 - 144 4.2e-19 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 202 - 270 5.2e-20 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 850
AccII CGCG 1 cut(s) 818
AciI CCGC 3 cut(s) 162, 791, 818
AclWI GGATC 4 cut(s) 208, 360, 373, 695
AcsI RAATTY 1 cut(s) 326
AcuI CTGAAG 4 cut(s) 111, 195, 329, 533
AfaI GTAC 3 cut(s) 314, 487, 822
AfiI CCNNNNNNNGG 3 cut(s) 18, 903, 910
AgsI TTSAA 3 cut(s) 323, 593, 1072
AhlI ACTAGT 1 cut(s) 190
AjnI CCWGG 3 cut(s) 25, 63, 879
AluBI AGCT 9 cut(s) 16, 42, 209, 307, 360, 466, 706, 808, 987
AluI AGCT 9 cut(s) 16, 42, 209, 307, 360, 466, 706, 808, 987
Alw21I GWGCWC 2 cut(s) 44, 873
AlwI GGATC 4 cut(s) 208, 360, 373, 695
AlwNI CAGNNNCTG 1 cut(s) 218
AoxI GGCC 1 cut(s) 658
ApeKI GCWGC 1 cut(s) 893
ApoI RAATTY 1 cut(s) 326
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 1 cut(s) 883
AsuC2I CCSGG 2 cut(s) 685, 905
AsuHPI GGTGA 2 cut(s) 64, 682
AsuII TTCGAA 1 cut(s) 120
AvaII GGWCC 1 cut(s) 883
AxyI CCTNAGG 1 cut(s) 479
BamHI GGATCC 1 cut(s) 365
BanII GRGCYC 1 cut(s) 44
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
Bbv12I GWGCWC 2 cut(s) 44, 873
BbvI GCAGC 1 cut(s) 880
BccI CCATC 6 cut(s) 182, 590, 701, 734, 1038, 1046
BceAI ACGGC 1 cut(s) 797
BcgI CGANNNNNNTGC 2 cut(s) 959, 993
BciT130I CCWGG 3 cut(s) 27, 65, 881
BcnI CCSGG 2 cut(s) 685, 905
BcuI ACTAGT 1 cut(s) 190
BfaI CTAG 4 cut(s) 45, 191, 371, 521
BfmI CTRYAG 3 cut(s) 112, 725, 1021
BisI GCNGC 1 cut(s) 894
BlsI GCNGC 1 cut(s) 895
Bme1390I CCNGG 5 cut(s) 27, 65, 685, 881, 905
Bme18I GGWCC 1 cut(s) 883
BmgT120I GGNCC 1 cut(s) 883
BmiI GGNNCC 3 cut(s) 367, 753, 949
BmrFI CCNGG 5 cut(s) 27, 65, 685, 881, 905
BmrI ACTGGG 1 cut(s) 409
BmsI GCATC 4 cut(s) 14, 69, 591, 763
BmuI ACTGGG 1 cut(s) 409
BpmI CTGGAG 3 cut(s) 86, 887, 966
Bpu14I TTCGAA 1 cut(s) 120
BpuMI CCSGG 2 cut(s) 685, 905
Bsa29I ATCGAT 1 cut(s) 177
BsaBI GATNNNNATC 2 cut(s) 692, 999
BsaJI CCNNGG 3 cut(s) 277, 342, 525
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 903, 910
Bse1I ACTGG 4 cut(s) 228, 404, 870, 1106
Bse21I CCTNAGG 1 cut(s) 479
Bse8I GATNNNNATC 2 cut(s) 692, 999
BseBI CCWGG 3 cut(s) 27, 65, 881
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 3 cut(s) 277, 342, 525
BseGI GGATG 7 cut(s) 60, 473, 606, 621, 693, 1049, 1057
BseJI GATNNNNATC 2 cut(s) 692, 999
BseLI CCNNNNNNNGG 3 cut(s) 18, 903, 910
BseMII CTCAG 1 cut(s) 493
BseNI ACTGG 4 cut(s) 228, 404, 870, 1106
BseRI GAGGAG 1 cut(s) 596
BseXI GCAGC 1 cut(s) 880
BseYI CCCAGC 1 cut(s) 12
Bsh1236I CGCG 1 cut(s) 818
BshFI GGCC 1 cut(s) 660
BshVI ATCGAT 1 cut(s) 177
BsiHKAI GWGCWC 2 cut(s) 44, 873
BsiSI CCGG 3 cut(s) 684, 897, 904
BslFI GGGAC 3 cut(s) 80, 324, 809
BslI CCNNNNNNNGG 3 cut(s) 18, 903, 910
BsmFI GGGAC 3 cut(s) 80, 324, 809
BsnI GGCC 1 cut(s) 660
Bsp119I TTCGAA 1 cut(s) 120
Bsp1286I GDGCHC 2 cut(s) 44, 873
Bsp143I GATC 6 cut(s) 69, 142, 200, 365, 687, 1000
Bsp19I CCATGG 2 cut(s) 277, 525
BspACI CCGC 3 cut(s) 162, 791, 818
BspANI GGCC 1 cut(s) 660
BspCNI CTCAG 1 cut(s) 492
BspDI ATCGAT 1 cut(s) 177
BspFNI CGCG 1 cut(s) 818
BspHI TCATGA 1 cut(s) 1029
BspLI GGNNCC 3 cut(s) 367, 753, 949
BspMAI CTGCAG 1 cut(s) 729
BspPI GGATC 4 cut(s) 208, 360, 373, 695
BspQI GCTCTTC 1 cut(s) 828
BspT104I TTCGAA 1 cut(s) 120
BsrI ACTGG 4 cut(s) 228, 404, 870, 1106
BssECI CCNNGG 3 cut(s) 277, 342, 525
BssMI GATC 6 cut(s) 69, 142, 200, 365, 687, 1000
BssNAI GTATAC 1 cut(s) 851
BssT1I CCWWGG 3 cut(s) 277, 342, 525
Bst1107I GTATAC 1 cut(s) 851
Bst2UI CCWGG 3 cut(s) 27, 65, 881
Bst4CI ACNGT 2 cut(s) 713, 925
Bst6I CTCTTC 4 cut(s) 342, 461, 822, 828
BstBI TTCGAA 1 cut(s) 120
BstC8I GCNNGC 2 cut(s) 634, 810
BstDEI CTNAG 1 cut(s) 479
BstDSI CCRYGG 2 cut(s) 277, 525
BstF5I GGATG 7 cut(s) 60, 473, 606, 621, 693, 1049, 1057
BstFNI CGCG 1 cut(s) 818
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 6 cut(s) 72, 145, 203, 368, 690, 1003
BstMBI GATC 6 cut(s) 69, 142, 200, 365, 687, 1000
BstMWI GCNNNNNNNGC 2 cut(s) 39, 243
BstNI CCWGG 3 cut(s) 27, 65, 881
BstNSI RCATGY 1 cut(s) 636
BstSCI CCNGG 5 cut(s) 25, 63, 683, 879, 903
BstSFI CTRYAG 3 cut(s) 112, 725, 1021
BstUI CGCG 1 cut(s) 818
BstV1I GCAGC 1 cut(s) 880
BstX2I RGATCY 1 cut(s) 365
BstYI RGATCY 1 cut(s) 365
BstZ17I GTATAC 1 cut(s) 851
Bsu15I ATCGAT 1 cut(s) 177
Bsu36I CCTNAGG 1 cut(s) 479
BsuRI GGCC 1 cut(s) 660
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 2 cut(s) 277, 525
BtsCI GGATG 7 cut(s) 60, 473, 606, 621, 693, 1049, 1057
BtsIMutI CAGTG 2 cut(s) 615, 863
Cac8I GCNNGC 2 cut(s) 634, 810
CaiI CAGNNNCTG 1 cut(s) 218
CciI TCATGA 1 cut(s) 1029
CfoI GCGC 1 cut(s) 237
Cfr13I GGNCC 1 cut(s) 883
ClaI ATCGAT 1 cut(s) 177
CseI GACGC 3 cut(s) 326, 686, 1073
Csp6I GTAC 3 cut(s) 313, 486, 821
CviAII CATG 7 cut(s) 278, 418, 424, 526, 633, 1030, 1040
CviQI GTAC 3 cut(s) 313, 486, 821
DdeI CTNAG 1 cut(s) 479
DpnI GATC 6 cut(s) 71, 144, 202, 367, 689, 1002
DpnII GATC 6 cut(s) 69, 142, 200, 365, 687, 1000
Eam1104I CTCTTC 4 cut(s) 342, 461, 822, 828
EarI CTCTTC 4 cut(s) 342, 461, 822, 828
Ecl136II GAGCTC 1 cut(s) 42
Eco130I CCWWGG 3 cut(s) 277, 342, 525
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 1 cut(s) 883
Eco53kI GAGCTC 1 cut(s) 42
Eco57I CTGAAG 4 cut(s) 111, 195, 329, 533
Eco81I CCTNAGG 1 cut(s) 479
EcoICRI GAGCTC 1 cut(s) 42
EcoRII CCWGG 3 cut(s) 25, 63, 879
EcoT14I CCWWGG 3 cut(s) 277, 342, 525
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 3 cut(s) 277, 342, 525
FaeI CATG 7 cut(s) 281, 421, 427, 529, 636, 1033, 1043
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FaqI GGGAC 3 cut(s) 80, 324, 809
FatI CATG 7 cut(s) 277, 417, 423, 525, 632, 1029, 1039
FauI CCCGC 1 cut(s) 784
FblI GTMKAC 1 cut(s) 850
Fnu4HI GCNGC 1 cut(s) 894
FokI GGATG 7 cut(s) 47, 460, 613, 628, 680, 1056, 1064
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 1 cut(s) 894
FspBI CTAG 4 cut(s) 45, 191, 371, 521
GlaI GCGC 1 cut(s) 236
GluI GCNGC 1 cut(s) 894
GsaI CCCAGC 1 cut(s) 16
GsuI CTGGAG 3 cut(s) 86, 887, 966
HaeIII GGCC 1 cut(s) 660
HapII CCGG 3 cut(s) 684, 897, 904
HgaI GACGC 3 cut(s) 326, 686, 1073
HhaI GCGC 1 cut(s) 237
Hin1II CATG 7 cut(s) 281, 421, 427, 529, 636, 1033, 1043
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HincII GTYRAC 2 cut(s) 760, 1063
HindII GTYRAC 2 cut(s) 760, 1063
HindIII AAGCTT 1 cut(s) 207
HinfI GANTC 7 cut(s) 80, 215, 258, 411, 494, 900, 1026
HpaII CCGG 3 cut(s) 684, 897, 904
HphI GGTGA 2 cut(s) 64, 682
Hpy166II GTNNAC 3 cut(s) 760, 851, 1063
Hpy188I TCNGA 6 cut(s) 142, 151, 205, 214, 220, 676
Hpy188III TCNNGA 3 cut(s) 481, 945, 1030
Hpy8I GTNNAC 3 cut(s) 760, 851, 1063
Hpy99I CGWCG 1 cut(s) 818
HpyAV CCTTC 3 cut(s) 480, 499, 544
HpyCH4III ACNGT 2 cut(s) 713, 925
HpyCH4IV ACGT 2 cut(s) 108, 394
HpyCH4V TGCA 5 cut(s) 246, 604, 632, 727, 863
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 243
HpyF3I CTNAG 1 cut(s) 479
HpySE526I ACGT 2 cut(s) 108, 394
Hsp92II CATG 7 cut(s) 281, 421, 427, 529, 636, 1033, 1043
HspAI GCGC 1 cut(s) 235
Kzo9I GATC 6 cut(s) 69, 142, 200, 365, 687, 1000
LguI GCTCTTC 1 cut(s) 828
LmnI GCTCC 3 cut(s) 471, 868, 947
Lsp1109I GCAGC 1 cut(s) 880
LweI GCATC 4 cut(s) 14, 69, 591, 763
MaeI CTAG 4 cut(s) 45, 191, 371, 521
MaeII ACGT 2 cut(s) 108, 394
MaeIII GTNAC 3 cut(s) 104, 395, 937
MalI GATC 6 cut(s) 71, 144, 202, 367, 689, 1002
MboI GATC 6 cut(s) 69, 142, 200, 365, 687, 1000
MboII GAAGA 9 cut(s) 134, 335, 359, 448, 571, 605, 782, 839, 845
MflI RGATCY 1 cut(s) 365
MhlI GDGCHC 2 cut(s) 44, 873
MluCI AATT 3 cut(s) 326, 619, 856
MlyI GAGTC 3 cut(s) 89, 503, 894
MmeI TCCRAC 1 cut(s) 1029
MseI TTAA 5 cut(s) 86, 333, 708, 732, 786
MslI CAYNNNNRTG 2 cut(s) 179, 422
MspI CCGG 3 cut(s) 684, 897, 904
MspR9I CCNGG 5 cut(s) 27, 65, 685, 881, 905
MvaI CCWGG 3 cut(s) 27, 65, 881
MvnI CGCG 1 cut(s) 818
MwoI GCNNNNNNNGC 2 cut(s) 39, 243
NciI CCSGG 2 cut(s) 685, 905
NcoI CCATGG 2 cut(s) 277, 525
NdeII GATC 6 cut(s) 69, 142, 200, 365, 687, 1000
NlaIII CATG 7 cut(s) 281, 421, 427, 529, 636, 1033, 1043
NlaIV GGNNCC 3 cut(s) 367, 753, 949
NmuCI GTSAC 2 cut(s) 395, 937
NspI RCATGY 1 cut(s) 636
NspV TTCGAA 1 cut(s) 120
PaeI GCATGC 1 cut(s) 636
PagI TCATGA 1 cut(s) 1029
PciSI GCTCTTC 1 cut(s) 828
PcsI WCGNNNNNNNCGW 1 cut(s) 822
PfeI GAWTC 4 cut(s) 215, 258, 411, 1026
PfoI TCCNGGA 3 cut(s) 63, 683, 903
PkrI GCNGC 1 cut(s) 895
PleI GAGTC 3 cut(s) 88, 502, 894
PpsI GAGTC 3 cut(s) 88, 502, 894
Psp124BI GAGCTC 1 cut(s) 44
Psp6I CCWGG 3 cut(s) 25, 63, 879
PspFI CCCAGC 1 cut(s) 12
PspGI CCWGG 3 cut(s) 25, 63, 879
PspN4I GGNNCC 3 cut(s) 367, 753, 949
PspPI GGNCC 1 cut(s) 883
PstI CTGCAG 1 cut(s) 729
PstNI CAGNNNCTG 1 cut(s) 218
PsuI RGATCY 1 cut(s) 365
RsaI GTAC 3 cut(s) 314, 487, 822
RsaNI GTAC 3 cut(s) 313, 486, 821
RseI CAYNNNNRTG 2 cut(s) 179, 422
SacI GAGCTC 1 cut(s) 44
SapI GCTCTTC 1 cut(s) 828
SaqAI TTAA 5 cut(s) 86, 333, 708, 732, 786
SatI GCNGC 1 cut(s) 894
Sau3AI GATC 6 cut(s) 69, 142, 200, 365, 687, 1000
Sau96I GGNCC 1 cut(s) 883
SchI GAGTC 3 cut(s) 89, 503, 894
ScrFI CCNGG 5 cut(s) 27, 65, 685, 881, 905
SduI GDGCHC 2 cut(s) 44, 873
SfaNI GCATC 4 cut(s) 14, 69, 591, 763
SfcI CTRYAG 3 cut(s) 112, 725, 1021
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 1 cut(s) 883
SmiMI CAYNNNNRTG 2 cut(s) 179, 422
SpeI ACTAGT 1 cut(s) 190
SphI GCATGC 1 cut(s) 636
Sse9I AATT 3 cut(s) 326, 619, 856
SsiI CCGC 3 cut(s) 162, 791, 818
SspMI CTAG 4 cut(s) 45, 191, 371, 521
SstI GAGCTC 1 cut(s) 44
StyD4I CCNGG 5 cut(s) 25, 63, 683, 879, 903
StyI CCWWGG 3 cut(s) 277, 342, 525
TaaI ACNGT 2 cut(s) 713, 925
TaiI ACGT 2 cut(s) 111, 397
TaqI TCGA 5 cut(s) 120, 177, 492, 825, 969
TasI AATT 3 cut(s) 326, 619, 856
TfiI GAWTC 4 cut(s) 215, 258, 411, 1026
Tru1I TTAA 5 cut(s) 86, 333, 708, 732, 786
Tru9I TTAA 5 cut(s) 86, 333, 708, 732, 786
TscAI CASTG 2 cut(s) 615, 870
TseFI GTSAC 2 cut(s) 395, 937
TseI GCWGC 1 cut(s) 893
Tsp45I GTSAC 2 cut(s) 395, 937
TspDTI ATGAA 7 cut(s) 126, 425, 440, 462, 632, 1018, 1046
TspGWI ACGGA 1 cut(s) 755
TspRI CASTG 2 cut(s) 615, 870
VpaK11BI GGWCC 1 cut(s) 883
XapI RAATTY 1 cut(s) 326
XceI RCATGY 1 cut(s) 636
XmiI GTMKAC 1 cut(s) 850
XspI CTAG 4 cut(s) 45, 191, 371, 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.