Rw2G028870

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001297
Physical Location & Seq
Reverse (-)
46343522 .. 46347328
3807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G028870.1

Sequence Viewer

Length: 1104 bp
ATGGTATCATCAAAACCAGCCAGCTGCTTTGGTCCCTCTCATCATCACTCAGGAGTCAGTATTATTGAGGAACTCATCACTGATGATTCAGTGATCGAAGTCCCGCAAAAGTATGTTATCCCAGACCAGGAGTCTCCAATCTCTCATGGGACTATCCTTGCCACAATTCCCACCATTGACATGAGACAGTTGGTCCTAATCAATCAGTTTGCAAACAACGATGAACTGGAGAAGTTGCACTCAACATGCAAAGAATGGGGAATTTTTCAGTTGGTTAACCATGGAGTTAGCTCTGCACAACTGGACAAAGTGAAGCAAGAATTAAAGGATTTTTTCAAGCTTCCCTTGGAAGAGAAAATGAGATACAAAGTAAGGCCAGGTGATGTTGAAGGCTATGGAACTGTTGTCAAATCGCAAGATCAAAAACTCGACTGGGGTAACAGGTTCTTCATGTTTACCAACCCGAGAAGGAAACCCCATCTTTTCCCGGAACTGCCTACATCCCTGAGGAATACCTTGGAGACATACATGTTGGAATTACAAAGGATTGCCCGAACCCTTTTAGGGTTCATGGGAAAACTTCTAAAGATGAAGATGGAGGAGATGGAAGAGTTGTTTGAAGATGGTCTGCAGTCACTGAGGATGACTCACTATCCTCAATGCCCAAAACCAGAGCTAGTTGTTGGCCTCAGGCCTCATTCTGATGGTAGTGGCATCACTATTCTCCACCAGGTTGATGGAGTTCAAGGCCTTCAAATTAAAAAAGATGGGGCTTGGTCCCCTGTGATCTTTGACCATGGTGCCTTTGTTGTCAATGTTGGAGACATTCTAGAGATTCTAAGTGATGGGCTTTATAAAAGTATCGAGCACAGAGTGACTGTGAACTCAGAGAAAGAAAGGATTTCAATTGCTATGTTCTTCATGCCCAAACTTGAGGCAGAAATTGGACCTGTGACCACCCTGAGAGATCCTCAGAAGCCACCATCTTCTGGGAAAATAGGAATGGAAAAATTTGTTGAGAAGTACTTTGCTCATAACCTAAATGGAAAAACACCCTTGGAGCAAATGAAAACAGAAGACAGTGAAGAGAAAACTACTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

367

Amino Acids

41.6

Weight (kDa)

5.94

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 56 - 159 4.6e-27 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 211 - 309 7.2e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 855
AccB1I GGYRCC 1 cut(s) 800
AccB7I CCANNNNNTGG 1 cut(s) 989
AciI CCGC 1 cut(s) 104
AclWI GGATC 1 cut(s) 962
AcsI RAATTY 2 cut(s) 261, 1010
AdeI CACNNNGTG 1 cut(s) 874
AfaI GTAC 1 cut(s) 1025
AfiI CCNNNNNNNGG 3 cut(s) 127, 564, 989
AflIII ACRYGT 1 cut(s) 528
AgsI TTSAA 6 cut(s) 337, 389, 620, 746, 755, 906
AhdI GACNNNNNGTC 2 cut(s) 130, 191
AjnI CCWGG 3 cut(s) 126, 376, 729
AluBI AGCT 4 cut(s) 24, 291, 340, 676
AluI AGCT 4 cut(s) 24, 291, 340, 676
Alw21I GWGCWC 1 cut(s) 870
Alw26I GTCTC 4 cut(s) 138, 178, 515, 816
AlwI GGATC 1 cut(s) 962
AlwNI CAGNNNCTG 1 cut(s) 637
Ama87I CYCGRG 1 cut(s) 463
AoxI GGCC 4 cut(s) 374, 685, 692, 748
ApeKI GCWGC 1 cut(s) 24
ApoI RAATTY 2 cut(s) 261, 1010
AspS9I GGNCC 4 cut(s) 32, 193, 777, 947
AsuC2I CCSGG 1 cut(s) 488
AsuHPI GGTGA 1 cut(s) 392
AvaI CYCGRG 1 cut(s) 463
AvaII GGWCC 4 cut(s) 32, 193, 777, 947
AxyI CCTNAGG 2 cut(s) 506, 689
BanI GGYRCC 1 cut(s) 800
BbsI GAAGAC 1 cut(s) 1083
Bbv12I GWGCWC 1 cut(s) 870
BbvI GCAGC 1 cut(s) 11
BccI CCATC 9 cut(s) 486, 589, 598, 617, 698, 731, 761, 839, 991
BciT130I CCWGG 3 cut(s) 128, 378, 731
BcnI CCSGG 1 cut(s) 488
BcoDI GTCTC 4 cut(s) 138, 178, 515, 816
BfaI CTAG 2 cut(s) 677, 830
BfmI CTRYAG 1 cut(s) 629
BisI GCNGC 1 cut(s) 25
BlsI GCNGC 1 cut(s) 26
BmcAI AGTACT 1 cut(s) 1025
Bme1390I CCNGG 4 cut(s) 128, 378, 488, 731
Bme18I GGWCC 4 cut(s) 32, 193, 777, 947
BmeRI GACNNNNNGTC 2 cut(s) 130, 191
BmeT110I CYCGRG 1 cut(s) 463
BmgT120I GGNCC 4 cut(s) 32, 193, 777, 947
BmiI GGNNCC 3 cut(s) 34, 779, 802
BmrFI CCNGG 4 cut(s) 128, 378, 488, 731
BmrI ACTGGG 1 cut(s) 442
BmsI GCATC 1 cut(s) 723
BmuI ACTGGG 1 cut(s) 442
BpiI GAAGAC 1 cut(s) 1083
BplI GAGNNNNNCTC 4 cut(s) 631, 663, 955, 987
BpmI CTGGAG 1 cut(s) 248
BpuEI CTTGAG 1 cut(s) 953
BpuMI CCSGG 1 cut(s) 488
BsaJI CCNNGG 5 cut(s) 280, 345, 516, 796, 1056
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 564, 989
Bse1I ACTGG 3 cut(s) 231, 306, 437
Bse21I CCTNAGG 2 cut(s) 506, 689
BseBI CCWGG 3 cut(s) 128, 378, 731
BseDI CCNNGG 5 cut(s) 280, 345, 516, 796, 1056
BseGI GGATG 2 cut(s) 500, 648
BseLI CCNNNNNNNGG 3 cut(s) 127, 564, 989
BseMII CTCAG 7 cut(s) 63, 497, 629, 703, 900, 953, 986
BseNI ACTGG 3 cut(s) 231, 306, 437
BseRI GAGGAG 1 cut(s) 614
BseXI GCAGC 1 cut(s) 11
BsgI GTGCAG 1 cut(s) 279
BshFI GGCC 4 cut(s) 376, 687, 694, 750
BshNI GGYRCC 1 cut(s) 800
BsiHKAI GWGCWC 1 cut(s) 870
BsiHKCI CYCGRG 1 cut(s) 463
BsiSI CCGG 1 cut(s) 488
BslFI GGGAC 4 cut(s) 18, 86, 163, 763
BslI CCNNNNNNNGG 3 cut(s) 127, 564, 989
BsmAI GTCTC 4 cut(s) 138, 178, 515, 816
BsmFI GGGAC 4 cut(s) 18, 86, 163, 763
BsnI GGCC 4 cut(s) 376, 687, 694, 750
BsoBI CYCGRG 1 cut(s) 463
Bsp1286I GDGCHC 1 cut(s) 870
Bsp143I GATC 4 cut(s) 93, 418, 786, 967
Bsp19I CCATGG 2 cut(s) 280, 796
BspACI CCGC 1 cut(s) 104
BspANI GGCC 4 cut(s) 376, 687, 694, 750
BspCNI CTCAG 7 cut(s) 62, 498, 630, 702, 899, 954, 985
BspLI GGNNCC 3 cut(s) 34, 779, 802
BspMAI CTGCAG 1 cut(s) 633
BspPI GGATC 1 cut(s) 962
BspT107I GGYRCC 1 cut(s) 800
BsrI ACTGG 3 cut(s) 231, 306, 437
BssECI CCNNGG 5 cut(s) 280, 345, 516, 796, 1056
BssMI GATC 4 cut(s) 93, 418, 786, 967
BssT1I CCWWGG 5 cut(s) 280, 345, 516, 796, 1056
Bst2UI CCWGG 3 cut(s) 128, 378, 731
Bst4CI ACNGT 4 cut(s) 189, 403, 880, 1082
Bst6I CTCTTC 3 cut(s) 345, 603, 1080
BstC8I GCNNGC 1 cut(s) 22
BstDEI CTNAG 8 cut(s) 49, 506, 638, 689, 839, 886, 962, 972
BstDSI CCRYGG 2 cut(s) 280, 796
BstF5I GGATG 2 cut(s) 500, 648
BstKTI GATC 4 cut(s) 96, 421, 789, 970
BstMAI GTCTC 4 cut(s) 138, 178, 515, 816
BstMBI GATC 4 cut(s) 93, 418, 786, 967
BstNI CCWGG 3 cut(s) 128, 378, 731
BstNSI RCATGY 2 cut(s) 249, 532
BstSCI CCNGG 4 cut(s) 126, 376, 486, 729
BstSFI CTRYAG 1 cut(s) 629
BstV1I GCAGC 1 cut(s) 11
BstV2I GAAGAC 1 cut(s) 1083
BstX2I RGATCY 1 cut(s) 967
BstXI CCANNNNNNTGG 1 cut(s) 737
BstYI RGATCY 1 cut(s) 967
Bsu36I CCTNAGG 2 cut(s) 506, 689
BsuRI GGCC 4 cut(s) 376, 687, 694, 750
BtgI CCRYGG 2 cut(s) 280, 796
BtsCI GGATG 2 cut(s) 500, 648
BtsIMutI CAGTG 4 cut(s) 78, 96, 635, 1087
Cac8I GCNNGC 1 cut(s) 22
CaiI CAGNNNCTG 1 cut(s) 637
Cfr13I GGNCC 4 cut(s) 32, 193, 777, 947
CsiI ACCWGGT 1 cut(s) 729
Csp6I GTAC 1 cut(s) 1024
CviQI GTAC 1 cut(s) 1024
DdeI CTNAG 8 cut(s) 49, 506, 638, 689, 839, 886, 962, 972
DpnI GATC 4 cut(s) 95, 420, 788, 969
DpnII GATC 4 cut(s) 93, 418, 786, 967
DraIII CACNNNGTG 1 cut(s) 874
DriI GACNNNNNGTC 2 cut(s) 130, 191
Eam1104I CTCTTC 3 cut(s) 345, 603, 1080
Eam1105I GACNNNNNGTC 2 cut(s) 130, 191
EarI CTCTTC 3 cut(s) 345, 603, 1080
Eco130I CCWWGG 5 cut(s) 280, 345, 516, 796, 1056
Eco147I AGGCCT 2 cut(s) 694, 750
Eco47I GGWCC 4 cut(s) 32, 193, 777, 947
Eco81I CCTNAGG 2 cut(s) 506, 689
Eco88I CYCGRG 1 cut(s) 463
EcoRII CCWGG 3 cut(s) 126, 376, 729
EcoT14I CCWWGG 5 cut(s) 280, 345, 516, 796, 1056
ErhI CCWWGG 5 cut(s) 280, 345, 516, 796, 1056
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 4 cut(s) 18, 86, 163, 763
FauI CCCGC 1 cut(s) 111
Fnu4HI GCNGC 1 cut(s) 25
FokI GGATG 2 cut(s) 487, 655
Fsp4HI GCNGC 1 cut(s) 25
FspBI CTAG 2 cut(s) 677, 830
GluI GCNGC 1 cut(s) 25
GsuI CTGGAG 1 cut(s) 248
HaeIII GGCC 4 cut(s) 376, 687, 694, 750
HapII CCGG 1 cut(s) 488
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 5 cut(s) 54, 86, 131, 646, 835
HpaI GTTAAC 1 cut(s) 277
HpaII CCGG 1 cut(s) 488
HphI GGTGA 1 cut(s) 392
Hpy166II GTNNAC 3 cut(s) 277, 456, 883
Hpy188I TCNGA 3 cut(s) 703, 889, 975
Hpy188III TCNNGA 2 cut(s) 51, 830
Hpy8I GTNNAC 3 cut(s) 277, 456, 883
HpyAV CCTTC 3 cut(s) 383, 462, 761
HpyCH4III ACNGT 4 cut(s) 189, 403, 880, 1082
HpyCH4V TGCA 6 cut(s) 212, 238, 249, 296, 631, 1100
HpyF3I CTNAG 8 cut(s) 49, 506, 638, 689, 839, 886, 962, 972
KspAI GTTAAC 1 cut(s) 277
Kzo9I GATC 4 cut(s) 93, 418, 786, 967
LmnI GCTCC 1 cut(s) 1060
Lsp1109I GCAGC 1 cut(s) 11
LweI GCATC 1 cut(s) 723
MabI ACCWGGT 1 cut(s) 729
MaeI CTAG 2 cut(s) 677, 830
MaeIII GTNAC 4 cut(s) 437, 633, 874, 952
MalI GATC 4 cut(s) 95, 420, 788, 969
MboI GATC 4 cut(s) 93, 418, 786, 967
MboII GAAGA 9 cut(s) 362, 439, 604, 620, 632, 910, 978, 1088, 1097
MfeI CAATTG 1 cut(s) 906
MflI RGATCY 1 cut(s) 967
MhlI GDGCHC 1 cut(s) 870
MluCI AATT 8 cut(s) 165, 261, 320, 536, 756, 906, 942, 1010
MlyI GAGTC 3 cut(s) 63, 140, 640
MmeI TCCRAC 2 cut(s) 513, 799
MseI TTAA 3 cut(s) 276, 323, 759
MslI CAYNNNNRTG 2 cut(s) 179, 702
MspA1I CMGCKG 1 cut(s) 24
MspI CCGG 1 cut(s) 488
MspR9I CCNGG 4 cut(s) 128, 378, 488, 731
MunI CAATTG 1 cut(s) 906
MvaI CCWGG 3 cut(s) 128, 378, 731
NciI CCSGG 1 cut(s) 488
NcoI CCATGG 2 cut(s) 280, 796
NdeII GATC 4 cut(s) 93, 418, 786, 967
NlaIV GGNNCC 3 cut(s) 34, 779, 802
NmuCI GTSAC 3 cut(s) 633, 874, 952
NspI RCATGY 2 cut(s) 249, 532
PceI AGGCCT 2 cut(s) 694, 750
PciI ACATGT 1 cut(s) 528
PfeI GAWTC 2 cut(s) 86, 835
PflMI CCANNNNNTGG 1 cut(s) 989
PfoI TCCNGGA 1 cut(s) 486
PkrI GCNGC 1 cut(s) 26
PleI GAGTC 3 cut(s) 62, 139, 640
PpsI GAGTC 3 cut(s) 62, 139, 640
PscI ACATGT 1 cut(s) 528
PsiI TTATAA 1 cut(s) 855
Psp6I CCWGG 3 cut(s) 126, 376, 729
PspGI CCWGG 3 cut(s) 126, 376, 729
PspN4I GGNNCC 3 cut(s) 34, 779, 802
PspPI GGNCC 4 cut(s) 32, 193, 777, 947
PstI CTGCAG 1 cut(s) 633
PstNI CAGNNNCTG 1 cut(s) 637
PsuI RGATCY 1 cut(s) 967
PvuII CAGCTG 1 cut(s) 24
RsaI GTAC 1 cut(s) 1025
RsaNI GTAC 1 cut(s) 1024
RseI CAYNNNNRTG 2 cut(s) 179, 702
SaqAI TTAA 3 cut(s) 276, 323, 759
SatI GCNGC 1 cut(s) 25
Sau3AI GATC 4 cut(s) 93, 418, 786, 967
Sau96I GGNCC 4 cut(s) 32, 193, 777, 947
ScaI AGTACT 1 cut(s) 1025
SchI GAGTC 3 cut(s) 63, 140, 640
ScrFI CCNGG 4 cut(s) 128, 378, 488, 731
SduI GDGCHC 1 cut(s) 870
SexAI ACCWGGT 1 cut(s) 729
SfaNI GCATC 1 cut(s) 723
SfcI CTRYAG 1 cut(s) 629
SinI GGWCC 4 cut(s) 32, 193, 777, 947
SmiMI CAYNNNNRTG 2 cut(s) 179, 702
SmlI CTYRAG 1 cut(s) 932
SmoI CTYRAG 1 cut(s) 932
Sse9I AATT 8 cut(s) 165, 261, 320, 536, 756, 906, 942, 1010
SseBI AGGCCT 2 cut(s) 694, 750
SsiI CCGC 1 cut(s) 104
SspMI CTAG 2 cut(s) 677, 830
StuI AGGCCT 2 cut(s) 694, 750
StyD4I CCNGG 4 cut(s) 126, 376, 486, 729
StyI CCWWGG 5 cut(s) 280, 345, 516, 796, 1056
TaaI ACNGT 4 cut(s) 189, 403, 880, 1082
TaqI TCGA 3 cut(s) 96, 429, 864
TasI AATT 8 cut(s) 165, 261, 320, 536, 756, 906, 942, 1010
TatI WGTACW 1 cut(s) 1023
TfiI GAWTC 2 cut(s) 86, 835
Tru1I TTAA 3 cut(s) 276, 323, 759
Tru9I TTAA 3 cut(s) 276, 323, 759
TscAI CASTG 4 cut(s) 85, 96, 642, 1087
TseFI GTSAC 3 cut(s) 633, 874, 952
TseI GCWGC 1 cut(s) 24
Tsp45I GTSAC 3 cut(s) 633, 874, 952
TspDTI ATGAA 6 cut(s) 237, 439, 559, 605, 910, 1082
TspRI CASTG 4 cut(s) 85, 96, 642, 1087
Van91I CCANNNNNTGG 1 cut(s) 989
VpaK11BI GGWCC 4 cut(s) 32, 193, 777, 947
XapI RAATTY 2 cut(s) 261, 1010
XbaI TCTAGA 1 cut(s) 829
XceI RCATGY 2 cut(s) 249, 532
XcmI CCANNNNNNNNNTGG 1 cut(s) 734
XspI CTAG 2 cut(s) 677, 830
ZrmI AGTACT 1 cut(s) 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.