Rorug05G0003800

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001297
Physical Location & Seq
Reverse (-)
255266 .. 256827
1562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0003800.1

Sequence Viewer

Length: 738 bp
ATGAGTTTGAGTTTGAGGAAGTTGGTGACGAAAGCAAACCCTAATTGTAATGGAAGTGAGGATGAGGATGGTAAGGCGAAGAGAGAGACGAGGGTGGCTCTGGGCAACGGAAGCGAGGTGCTGTTGATTCCAAGGTTCATAAGCTTCGATCGGGCTTGGAATTGGTTCGATTACCTCAACACACATGTTCCCTGGACCAGACCCACTATTCGTGTCTTCGGCCGATCCTGCCTCCAGCCTAGAGATACATGTTATGTTGCAAGTCCGGGACTACCACAATTGGTTTACAGTGGATACCAACCTCATGCTTATTCATGGGATGATCTCCCTCCTCTTAAGCAACTCTTGGAAGCGGTCCATAAAGCTCTTCCTGGCAGCAGTTTCAATAGCTTGCTCTTAAATAGGTATAAAGGTGGTAATGACTATGTTGCTTGGCATTCTGATGATGAAAAGGTTTATGGTTCAACTCCTGAAATTGCGTCCCTATCCTTTGGATGCGAAAGGGAATTCTACTTGAAGAAGAAACCCTGTAAAACATCTCAAGAAAGAAAAAGTGATCAAGAAACTACAAGTAAGCGATTAAAGAGGAATCATGATGATCAGCACTCTTTCATGCTTAAGCATGGATCGCTCTTGGTGATGAGAGGTTATACTCAAAGGGATTGGATGCACTCTGTGCCTAAGCGGACAAAATTAGAGGCCACTCGTGTCAACCTCACCTTCAGGCATGTGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

245

Amino Acids

28.25

Weight (kDa)

9.46

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 40 - 242 1e-31 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 353, 685
AclWI GGATC 2 cut(s) 219, 634
AcoI YGGCCR 1 cut(s) 220
AcsI RAATTY 1 cut(s) 506
AcuI CTGAAG 1 cut(s) 706
AdeI CACNNNGTG 1 cut(s) 676
AflII CTTAAG 2 cut(s) 335, 617
AflIII ACRYGT 2 cut(s) 184, 248
AgsI TTSAA 3 cut(s) 385, 465, 517
AjnI CCWGG 2 cut(s) 191, 370
AluBI AGCT 3 cut(s) 144, 365, 390
AluI AGCT 3 cut(s) 144, 365, 390
Alw21I GWGCWC 1 cut(s) 735
Alw26I GTCTC 1 cut(s) 80
AlwI GGATC 2 cut(s) 219, 634
AoxI GGCC 2 cut(s) 220, 699
ApeKI GCWGC 1 cut(s) 375
ApoI RAATTY 1 cut(s) 506
ArsI GACNNNNNNTTYG 2 cut(s) 192, 224
Asp700I GAANNNNTTC 1 cut(s) 164
AspS9I GGNCC 2 cut(s) 195, 355
AsuC2I CCSGG 1 cut(s) 267
AsuHPI GGTGA 3 cut(s) 37, 649, 709
AvaII GGWCC 2 cut(s) 195, 355
BauI CACGAG 1 cut(s) 705
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 735
BbvI GCAGC 1 cut(s) 387
BccI CCATC 1 cut(s) 62
BciT130I CCWGG 2 cut(s) 193, 372
BciVI GTATCC 1 cut(s) 287
BclI TGATCA 2 cut(s) 556, 598
BcnI CCSGG 1 cut(s) 267
BcoDI GTCTC 1 cut(s) 80
BfaI CTAG 1 cut(s) 240
BfrI CTTAAG 2 cut(s) 335, 617
BfuI GTATCC 1 cut(s) 287
BisI GCNGC 1 cut(s) 376
BlsI GCNGC 1 cut(s) 377
Bme1390I CCNGG 3 cut(s) 193, 267, 372
Bme18I GGWCC 2 cut(s) 195, 355
BmgT120I GGNCC 2 cut(s) 195, 355
BmrFI CCNGG 3 cut(s) 193, 267, 372
BmsI GCATC 2 cut(s) 485, 657
BpiI GAAGAC 1 cut(s) 208
BplI GAGNNNNNCTC 2 cut(s) 82, 114
BpmI CTGGAG 1 cut(s) 218
Bpu10I CCTNAGC 1 cut(s) 681
BpuEI CTTGAG 1 cut(s) 525
BpuMI CCSGG 1 cut(s) 267
BsaJI CCNNGG 2 cut(s) 131, 191
BseBI CCWGG 2 cut(s) 193, 372
BseDI CCNNGG 2 cut(s) 131, 191
BseGI GGATG 5 cut(s) 67, 73, 325, 500, 672
BseRI GAGGAG 1 cut(s) 321
BseX3I CGGCCG 1 cut(s) 220
BseXI GCAGC 1 cut(s) 387
Bsh1285I CGRYCG 2 cut(s) 151, 223
BshFI GGCC 2 cut(s) 222, 701
BsiEI CGRYCG 2 cut(s) 151, 223
BsiHKAI GWGCWC 1 cut(s) 735
BsiSI CCGG 1 cut(s) 266
BslFI GGGAC 2 cut(s) 282, 466
BsmAI GTCTC 1 cut(s) 80
BsmBI CGTCTC 1 cut(s) 80
BsmFI GGGAC 2 cut(s) 282, 466
BsmI GAATGC 1 cut(s) 436
BsnI GGCC 2 cut(s) 222, 701
Bsp1286I GDGCHC 1 cut(s) 735
Bsp143I GATC 6 cut(s) 148, 224, 322, 556, 598, 626
BspACI CCGC 2 cut(s) 353, 685
BspANI GGCC 2 cut(s) 222, 701
BspHI TCATGA 1 cut(s) 592
BspPI GGATC 2 cut(s) 219, 634
BspQI GCTCTTC 1 cut(s) 372
BspTI CTTAAG 2 cut(s) 335, 617
BssECI CCNNGG 2 cut(s) 131, 191
BssMI GATC 6 cut(s) 148, 224, 322, 556, 598, 626
BssSI CACGAG 1 cut(s) 705
BssT1I CCWWGG 1 cut(s) 131
Bst2BI CACGAG 1 cut(s) 705
Bst2UI CCWGG 2 cut(s) 193, 372
Bst4CI ACNGT 1 cut(s) 290
Bst6I CTCTTC 2 cut(s) 74, 372
BstAFI CTTAAG 2 cut(s) 335, 617
BstAPI GCANNNNNTGC 1 cut(s) 676
BstC8I GCNNGC 1 cut(s) 392
BstDEI CTNAG 1 cut(s) 681
BstF5I GGATG 5 cut(s) 67, 73, 325, 500, 672
BstKTI GATC 6 cut(s) 151, 227, 325, 559, 601, 629
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 6 cut(s) 148, 224, 322, 556, 598, 626
BstMCI CGRYCG 2 cut(s) 151, 223
BstMWI GCNNNNNNNGC 4 cut(s) 111, 228, 628, 676
BstNI CCWGG 2 cut(s) 193, 372
BstNSI RCATGY 3 cut(s) 188, 252, 731
BstSCI CCNGG 3 cut(s) 191, 265, 370
BstV1I GCAGC 1 cut(s) 387
BstV2I GAAGAC 1 cut(s) 208
BstZI CGGCCG 1 cut(s) 220
BsuI GTATCC 1 cut(s) 287
BsuRI GGCC 2 cut(s) 222, 701
BtsCI GGATG 5 cut(s) 67, 73, 325, 500, 672
BtsIMutI CAGTG 1 cut(s) 295
Cac8I GCNNGC 1 cut(s) 392
CciI TCATGA 1 cut(s) 592
Cfr13I GGNCC 2 cut(s) 195, 355
CseI GACGC 1 cut(s) 468
CviAII CATG 8 cut(s) 185, 249, 305, 315, 593, 613, 623, 728
CviJI RGCY 8 cut(s) 98, 144, 155, 222, 238, 365, 390, 701
CviKI_1 RGCY 8 cut(s) 98, 144, 155, 222, 238, 365, 390, 701
DdeI CTNAG 1 cut(s) 681
DpnI GATC 6 cut(s) 150, 226, 324, 558, 600, 628
DpnII GATC 6 cut(s) 148, 224, 322, 556, 598, 626
DraIII CACNNNGTG 1 cut(s) 676
EaeI YGGCCR 1 cut(s) 220
EagI CGGCCG 1 cut(s) 220
Eam1104I CTCTTC 2 cut(s) 74, 372
EarI CTCTTC 2 cut(s) 74, 372
EclXI CGGCCG 1 cut(s) 220
Eco130I CCWWGG 1 cut(s) 131
Eco47I GGWCC 2 cut(s) 195, 355
Eco52I CGGCCG 1 cut(s) 220
Eco57I CTGAAG 1 cut(s) 706
EcoRI GAATTC 1 cut(s) 506
EcoRII CCWGG 2 cut(s) 191, 370
EcoT14I CCWWGG 1 cut(s) 131
ErhI CCWWGG 1 cut(s) 131
Esp3I CGTCTC 1 cut(s) 80
FaeI CATG 8 cut(s) 188, 252, 308, 318, 596, 616, 626, 731
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 2 cut(s) 282, 466
FatI CATG 8 cut(s) 184, 248, 304, 314, 592, 612, 622, 727
FbaI TGATCA 2 cut(s) 556, 598
Fnu4HI GCNGC 1 cut(s) 376
FokI GGATG 5 cut(s) 74, 80, 332, 507, 679
Fsp4HI GCNGC 1 cut(s) 376
FspBI CTAG 1 cut(s) 240
GluI GCNGC 1 cut(s) 376
GsuI CTGGAG 1 cut(s) 218
HaeIII GGCC 2 cut(s) 222, 701
HapII CCGG 1 cut(s) 266
HgaI GACGC 1 cut(s) 468
Hin1II CATG 8 cut(s) 188, 252, 308, 318, 596, 616, 626, 731
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HindIII AAGCTT 1 cut(s) 142
HinfI GANTC 2 cut(s) 127, 589
HpaII CCGG 1 cut(s) 266
HphI GGTGA 3 cut(s) 37, 649, 709
Hpy166II GTNNAC 2 cut(s) 286, 712
Hpy188I TCNGA 2 cut(s) 442, 737
Hpy188III TCNNGA 4 cut(s) 470, 542, 560, 593
Hpy8I GTNNAC 2 cut(s) 286, 712
HpyAV CCTTC 1 cut(s) 730
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4V TGCA 2 cut(s) 260, 670
HpyF10VI GCNNNNNNNGC 4 cut(s) 111, 228, 628, 676
HpyF3I CTNAG 1 cut(s) 681
Hsp92II CATG 8 cut(s) 188, 252, 308, 318, 596, 616, 626, 731
Ksp22I TGATCA 2 cut(s) 556, 598
Kzo9I GATC 6 cut(s) 148, 224, 322, 556, 598, 626
LguI GCTCTTC 1 cut(s) 372
Lsp1109I GCAGC 1 cut(s) 387
LweI GCATC 2 cut(s) 485, 657
MaeI CTAG 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 25
MalI GATC 6 cut(s) 150, 226, 324, 558, 600, 628
MboI GATC 6 cut(s) 148, 224, 322, 556, 598, 626
MboII GAAGA 5 cut(s) 91, 208, 359, 529, 532
MfeI CAATTG 1 cut(s) 278
MhlI GDGCHC 1 cut(s) 735
MluCI AATT 6 cut(s) 43, 160, 278, 474, 506, 692
MroXI GAANNNNTTC 1 cut(s) 164
MseI TTAA 4 cut(s) 336, 398, 581, 618
MslI CAYNNNNRTG 1 cut(s) 441
MspCI CTTAAG 2 cut(s) 335, 617
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 3 cut(s) 193, 267, 372
MunI CAATTG 1 cut(s) 278
Mva1269I GAATGC 1 cut(s) 436
MvaI CCWGG 2 cut(s) 193, 372
MwoI GCNNNNNNNGC 4 cut(s) 111, 228, 628, 676
NciI CCSGG 1 cut(s) 267
NdeII GATC 6 cut(s) 148, 224, 322, 556, 598, 626
NlaIII CATG 8 cut(s) 188, 252, 308, 318, 596, 616, 626, 731
NmuCI GTSAC 1 cut(s) 25
NspI RCATGY 3 cut(s) 188, 252, 731
PagI TCATGA 1 cut(s) 592
PciI ACATGT 2 cut(s) 184, 248
PciSI GCTCTTC 1 cut(s) 372
PctI GAATGC 1 cut(s) 436
PdmI GAANNNNTTC 1 cut(s) 164
PfeI GAWTC 2 cut(s) 127, 589
PfoI TCCNGGA 1 cut(s) 265
PkrI GCNGC 1 cut(s) 377
Ple19I CGATCG 1 cut(s) 151
PscI ACATGT 2 cut(s) 184, 248
Psp6I CCWGG 2 cut(s) 191, 370
PspGI CCWGG 2 cut(s) 191, 370
PspPI GGNCC 2 cut(s) 195, 355
PvuI CGATCG 1 cut(s) 151
RseI CAYNNNNRTG 1 cut(s) 441
SapI GCTCTTC 1 cut(s) 372
SaqAI TTAA 4 cut(s) 336, 398, 581, 618
SatI GCNGC 1 cut(s) 376
Sau3AI GATC 6 cut(s) 148, 224, 322, 556, 598, 626
Sau96I GGNCC 2 cut(s) 195, 355
ScrFI CCNGG 3 cut(s) 193, 267, 372
SduI GDGCHC 1 cut(s) 735
SfaNI GCATC 2 cut(s) 485, 657
SinI GGWCC 2 cut(s) 195, 355
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 3 cut(s) 335, 540, 617
SmoI CTYRAG 3 cut(s) 335, 540, 617
Sse9I AATT 6 cut(s) 43, 160, 278, 474, 506, 692
SsiI CCGC 2 cut(s) 353, 685
SspMI CTAG 1 cut(s) 240
StyD4I CCNGG 3 cut(s) 191, 265, 370
StyI CCWWGG 1 cut(s) 131
TaaI ACNGT 1 cut(s) 290
TaqI TCGA 2 cut(s) 147, 168
TasI AATT 6 cut(s) 43, 160, 278, 474, 506, 692
TfiI GAWTC 2 cut(s) 127, 589
Tru1I TTAA 4 cut(s) 336, 398, 581, 618
Tru9I TTAA 4 cut(s) 336, 398, 581, 618
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 1 cut(s) 375
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 4 cut(s) 127, 303, 462, 601
TspGWI ACGGA 1 cut(s) 123
TspRI CASTG 1 cut(s) 295
Vha464I CTTAAG 2 cut(s) 335, 617
VpaK11BI GGWCC 2 cut(s) 195, 355
XapI RAATTY 1 cut(s) 506
XceI RCATGY 3 cut(s) 188, 252, 731
XmnI GAANNNNTTC 1 cut(s) 164
XspI CTAG 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.