Rh2AG356000

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001297
Physical Location & Seq
Reverse (-)
52469385 .. 52473194
3810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG356000.1

Sequence Viewer

Length: 1077 bp
ATGGTATCATCAAAACCAGCCTGCTTCGGCCCCTCTCATCATCACTCAGGAGTCAGTTGTATTGAGGAATTCGTCACCGATGATTCAGTGATCGAAGTCCCGCAAAATTATGTTATCTCAGATCAGGAGTCTCCAATCTCTGATGGGACTATCCTTGCCACAATCCCCACCATTGACATGAGACAGTTGGTCCTAATCAATCAGTCTGCAAACAACGATGAACTTGAGAAGTTGCACTCAACATGCAAAGAATGGGGAATTTTTCAGTTGGTTAACCATGGAGTTAGCTCTGCACTACTGGACAAAGTGAAGCAAGAATTAGAGGATTTTTTCAAGCTTCCCTTGGAAGAGAAAATGAGATACAAAGTAAGGCCAGGTGATGTTGAAGGCTATGGAACTGTGGTCAAATCCCAAGATCAAAAACTCGACTGGGGTAACAGGTTCTTCATGTTTACCAACCCGAGAAGGAAACCCCATCTTTTCCCGGAGCTCCCTACATCCCTGAGGTCGAATACCTTGGAGACATACATGTTGGAATTACAAAGGATTGCCCGGACCCTTTTAGGGTTGATGGGAAAACTTCTAAAGATGAAGATGGAGGATATGGAAAAGTTGTTTGAAGATGGTCTGCAGTCACTGAGGATGACTCACTATCCTCCATGCCCAAAACCAGAACTAGTTATTGGCCTCAGGCCTCATTCTGATGGTAGTGGCATCACTATTCTCCACCAGGTTGGTGGAGTTCAAGGCCTTCAAATTAAAAAAGATGGGGCTTGGTTTCCGGTGATGTTTGACCATGGTGCCTTTGTTGTCAATGTTGGAGACATTCTAGAGATTCTGAGTGATGGGCTTTATAAAAGTATCGAGCACAAAGTGACTGTGAACCCAGAGAATGAAAGGATTTCAATTGCTATGTTCTTCATGCCCAAACTTGAGGCAGAAATTGGACCTGTGACCGCCATGAGAGATCCTCAGAAGCCACCATCTTATGGGAAAATAAGAATGGAAAAATTTGTGGAGAAGTACTTTGCTCATAACCTTAATGGAAAAACACCCTTGGAGCGAATGAAAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

358

Amino Acids

40.61

Weight (kDa)

6.2

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 55 - 158 4.9e-28 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 211 - 309 8.4e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 855
AccB1I GGYRCC 1 cut(s) 800
AccB7I CCANNNNNTGG 1 cut(s) 989
AciI CCGC 2 cut(s) 101, 957
AclWI GGATC 1 cut(s) 962
AcsI RAATTY 3 cut(s) 68, 258, 1010
AdeI CACNNNGTG 1 cut(s) 874
AfaI GTAC 1 cut(s) 1025
AfiI CCNNNNNNNGG 2 cut(s) 564, 989
AflIII ACRYGT 1 cut(s) 528
AgsI TTSAA 6 cut(s) 334, 386, 620, 746, 755, 906
AhdI GACNNNNNGTC 1 cut(s) 188
AhlI ACTAGT 1 cut(s) 676
AjnI CCWGG 2 cut(s) 373, 729
AjuI GAANNNNNNNTTGG 2 cut(s) 666, 698
AluBI AGCT 3 cut(s) 288, 337, 490
AluI AGCT 3 cut(s) 288, 337, 490
Alw21I GWGCWC 2 cut(s) 492, 870
Alw26I GTCTC 4 cut(s) 135, 175, 515, 816
AlwI GGATC 1 cut(s) 962
AlwNI CAGNNNCTG 1 cut(s) 637
Ama87I CYCGRG 1 cut(s) 460
AoxI GGCC 5 cut(s) 28, 371, 685, 692, 748
ApoI RAATTY 3 cut(s) 68, 258, 1010
AspS9I GGNCC 4 cut(s) 29, 190, 555, 947
AsuC2I CCSGG 2 cut(s) 485, 553
AsuHPI GGTGA 3 cut(s) 67, 389, 796
AvaI CYCGRG 1 cut(s) 460
AvaII GGWCC 3 cut(s) 190, 555, 947
AxyI CCTNAGG 2 cut(s) 503, 689
BanI GGYRCC 1 cut(s) 800
BanII GRGCYC 1 cut(s) 492
Bbv12I GWGCWC 2 cut(s) 492, 870
BccI CCATC 9 cut(s) 137, 483, 565, 589, 617, 698, 761, 839, 991
BciT130I CCWGG 2 cut(s) 375, 731
BcnI CCSGG 2 cut(s) 485, 553
BcoDI GTCTC 4 cut(s) 135, 175, 515, 816
BcuI ACTAGT 1 cut(s) 676
BfaI CTAG 2 cut(s) 677, 830
BfmI CTRYAG 1 cut(s) 629
BmcAI AGTACT 1 cut(s) 1025
Bme1390I CCNGG 4 cut(s) 375, 485, 553, 731
Bme18I GGWCC 3 cut(s) 190, 555, 947
BmeRI GACNNNNNGTC 1 cut(s) 188
BmeT110I CYCGRG 1 cut(s) 460
BmgT120I GGNCC 4 cut(s) 29, 190, 555, 947
BmiI GGNNCC 3 cut(s) 31, 557, 802
BmrFI CCNGG 4 cut(s) 375, 485, 553, 731
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 1 cut(s) 723
BmuI ACTGGG 1 cut(s) 439
BplI GAGNNNNNCTC 4 cut(s) 631, 663, 955, 987
BpuEI CTTGAG 2 cut(s) 245, 953
BpuMI CCSGG 2 cut(s) 485, 553
BsaJI CCNNGG 5 cut(s) 277, 342, 516, 796, 1056
BsaWI WCCGGW 1 cut(s) 781
Bsc4I CCNNNNNNNGG 2 cut(s) 564, 989
Bse1I ACTGG 2 cut(s) 303, 434
Bse21I CCTNAGG 2 cut(s) 503, 689
BseBI CCWGG 2 cut(s) 375, 731
BseDI CCNNGG 5 cut(s) 277, 342, 516, 796, 1056
BseGI GGATG 2 cut(s) 497, 648
BseLI CCNNNNNNNGG 2 cut(s) 564, 989
BseMII CTCAG 7 cut(s) 60, 132, 494, 629, 703, 830, 986
BseNI ACTGG 2 cut(s) 303, 434
BsgI GTGCAG 1 cut(s) 276
BshFI GGCC 5 cut(s) 30, 373, 687, 694, 750
BshNI GGYRCC 1 cut(s) 800
BsiHKAI GWGCWC 2 cut(s) 492, 870
BsiHKCI CYCGRG 1 cut(s) 460
BsiSI CCGG 3 cut(s) 485, 553, 782
BslFI GGGAC 2 cut(s) 83, 160
BslI CCNNNNNNNGG 2 cut(s) 564, 989
BsmAI GTCTC 4 cut(s) 135, 175, 515, 816
BsmFI GGGAC 2 cut(s) 83, 160
BsnI GGCC 5 cut(s) 30, 373, 687, 694, 750
BsoBI CYCGRG 1 cut(s) 460
Bsp1286I GDGCHC 2 cut(s) 492, 870
Bsp143I GATC 4 cut(s) 90, 121, 415, 967
Bsp19I CCATGG 2 cut(s) 277, 796
BspACI CCGC 2 cut(s) 101, 957
BspANI GGCC 5 cut(s) 30, 373, 687, 694, 750
BspCNI CTCAG 7 cut(s) 59, 131, 495, 630, 702, 831, 985
BspLI GGNNCC 3 cut(s) 31, 557, 802
BspMAI CTGCAG 1 cut(s) 633
BspPI GGATC 1 cut(s) 962
BspT107I GGYRCC 1 cut(s) 800
BsrI ACTGG 2 cut(s) 303, 434
BssECI CCNNGG 5 cut(s) 277, 342, 516, 796, 1056
BssMI GATC 4 cut(s) 90, 121, 415, 967
BssT1I CCWWGG 5 cut(s) 277, 342, 516, 796, 1056
Bst2UI CCWGG 2 cut(s) 375, 731
Bst4CI ACNGT 3 cut(s) 186, 400, 880
Bst6I CTCTTC 1 cut(s) 342
BstC8I GCNNGC 1 cut(s) 22
BstDEI CTNAG 7 cut(s) 46, 118, 503, 638, 689, 839, 972
BstDSI CCRYGG 2 cut(s) 277, 796
BstF5I GGATG 2 cut(s) 497, 648
BstKTI GATC 4 cut(s) 93, 124, 418, 970
BstMAI GTCTC 4 cut(s) 135, 175, 515, 816
BstMBI GATC 4 cut(s) 90, 121, 415, 967
BstNI CCWGG 2 cut(s) 375, 731
BstNSI RCATGY 2 cut(s) 246, 532
BstSCI CCNGG 4 cut(s) 373, 483, 551, 729
BstSFI CTRYAG 1 cut(s) 629
BstX2I RGATCY 1 cut(s) 967
BstXI CCANNNNNNTGG 2 cut(s) 734, 737
BstYI RGATCY 1 cut(s) 967
Bsu36I CCTNAGG 2 cut(s) 503, 689
BsuRI GGCC 5 cut(s) 30, 373, 687, 694, 750
BtgI CCRYGG 2 cut(s) 277, 796
BtsCI GGATG 2 cut(s) 497, 648
BtsIMutI CAGTG 2 cut(s) 93, 635
Cac8I GCNNGC 1 cut(s) 22
CaiI CAGNNNCTG 1 cut(s) 637
Cfr13I GGNCC 4 cut(s) 29, 190, 555, 947
CsiI ACCWGGT 1 cut(s) 729
Csp6I GTAC 1 cut(s) 1024
CviAII CATG 9 cut(s) 178, 243, 278, 448, 529, 660, 797, 922, 961
CviQI GTAC 1 cut(s) 1024
DdeI CTNAG 7 cut(s) 46, 118, 503, 638, 689, 839, 972
DpnI GATC 4 cut(s) 92, 123, 417, 969
DpnII GATC 4 cut(s) 90, 121, 415, 967
DraIII CACNNNGTG 1 cut(s) 874
DriI GACNNNNNGTC 1 cut(s) 188
Eam1104I CTCTTC 1 cut(s) 342
Eam1105I GACNNNNNGTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 342
Ecl136II GAGCTC 1 cut(s) 490
Eco130I CCWWGG 5 cut(s) 277, 342, 516, 796, 1056
Eco147I AGGCCT 2 cut(s) 694, 750
Eco24I GRGCYC 1 cut(s) 492
Eco47I GGWCC 3 cut(s) 190, 555, 947
Eco53kI GAGCTC 1 cut(s) 490
Eco81I CCTNAGG 2 cut(s) 503, 689
Eco88I CYCGRG 1 cut(s) 460
EcoICRI GAGCTC 1 cut(s) 490
EcoRI GAATTC 1 cut(s) 68
EcoRII CCWGG 2 cut(s) 373, 729
EcoT14I CCWWGG 5 cut(s) 277, 342, 516, 796, 1056
EcoT38I GRGCYC 1 cut(s) 492
ErhI CCWWGG 5 cut(s) 277, 342, 516, 796, 1056
FaeI CATG 9 cut(s) 181, 246, 281, 451, 532, 663, 800, 925, 964
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FaqI GGGAC 2 cut(s) 83, 160
FatI CATG 9 cut(s) 177, 242, 277, 447, 528, 659, 796, 921, 960
FauI CCCGC 1 cut(s) 108
FokI GGATG 2 cut(s) 484, 655
FriOI GRGCYC 1 cut(s) 492
FspBI CTAG 2 cut(s) 677, 830
HaeIII GGCC 5 cut(s) 30, 373, 687, 694, 750
HapII CCGG 3 cut(s) 485, 553, 782
Hin1II CATG 9 cut(s) 181, 246, 281, 451, 532, 663, 800, 925, 964
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 5 cut(s) 51, 83, 128, 646, 835
HpaI GTTAAC 1 cut(s) 274
HpaII CCGG 3 cut(s) 485, 553, 782
HphI GGTGA 3 cut(s) 67, 389, 796
Hpy166II GTNNAC 3 cut(s) 274, 453, 883
Hpy188I TCNGA 5 cut(s) 121, 142, 703, 840, 975
Hpy188III TCNNGA 3 cut(s) 48, 125, 830
Hpy8I GTNNAC 3 cut(s) 274, 453, 883
HpyAV CCTTC 3 cut(s) 380, 459, 761
HpyCH4III ACNGT 3 cut(s) 186, 400, 880
HpyCH4V TGCA 5 cut(s) 209, 235, 246, 293, 631
HpyF3I CTNAG 7 cut(s) 46, 118, 503, 638, 689, 839, 972
Hsp92II CATG 9 cut(s) 181, 246, 281, 451, 532, 663, 800, 925, 964
KspAI GTTAAC 1 cut(s) 274
Kzo9I GATC 4 cut(s) 90, 121, 415, 967
LmnI GCTCC 3 cut(s) 487, 495, 1060
LweI GCATC 1 cut(s) 723
MabI ACCWGGT 1 cut(s) 729
MaeI CTAG 2 cut(s) 677, 830
MaeIII GTNAC 5 cut(s) 73, 434, 633, 874, 952
MalI GATC 4 cut(s) 92, 123, 417, 969
MboI GATC 4 cut(s) 90, 121, 415, 967
MboII GAAGA 5 cut(s) 359, 436, 604, 632, 910
MfeI CAATTG 1 cut(s) 906
MflI RGATCY 1 cut(s) 967
MhlI GDGCHC 2 cut(s) 492, 870
MluCI AATT 9 cut(s) 68, 106, 258, 317, 536, 756, 906, 942, 1010
MlyI GAGTC 3 cut(s) 60, 137, 640
MmeI TCCRAC 2 cut(s) 513, 799
MseI TTAA 3 cut(s) 273, 759, 1041
MslI CAYNNNNRTG 2 cut(s) 176, 702
MspI CCGG 3 cut(s) 485, 553, 782
MspR9I CCNGG 4 cut(s) 375, 485, 553, 731
MunI CAATTG 1 cut(s) 906
MvaI CCWGG 2 cut(s) 375, 731
NciI CCSGG 2 cut(s) 485, 553
NcoI CCATGG 2 cut(s) 277, 796
NdeII GATC 4 cut(s) 90, 121, 415, 967
NlaIII CATG 9 cut(s) 181, 246, 281, 451, 532, 663, 800, 925, 964
NlaIV GGNNCC 3 cut(s) 31, 557, 802
NmuCI GTSAC 4 cut(s) 73, 633, 874, 952
NspI RCATGY 2 cut(s) 246, 532
PceI AGGCCT 2 cut(s) 694, 750
PciI ACATGT 1 cut(s) 528
PfeI GAWTC 2 cut(s) 83, 835
PflMI CCANNNNNTGG 1 cut(s) 989
PfoI TCCNGGA 1 cut(s) 483
PleI GAGTC 3 cut(s) 59, 136, 640
PpsI GAGTC 3 cut(s) 59, 136, 640
PscI ACATGT 1 cut(s) 528
PsiI TTATAA 1 cut(s) 855
Psp124BI GAGCTC 1 cut(s) 492
Psp6I CCWGG 2 cut(s) 373, 729
PspGI CCWGG 2 cut(s) 373, 729
PspN4I GGNNCC 3 cut(s) 31, 557, 802
PspPI GGNCC 4 cut(s) 29, 190, 555, 947
PstI CTGCAG 1 cut(s) 633
PstNI CAGNNNCTG 1 cut(s) 637
PsuI RGATCY 1 cut(s) 967
RsaI GTAC 1 cut(s) 1025
RsaNI GTAC 1 cut(s) 1024
RseI CAYNNNNRTG 2 cut(s) 176, 702
SacI GAGCTC 1 cut(s) 492
SaqAI TTAA 3 cut(s) 273, 759, 1041
Sau3AI GATC 4 cut(s) 90, 121, 415, 967
Sau96I GGNCC 4 cut(s) 29, 190, 555, 947
ScaI AGTACT 1 cut(s) 1025
SchI GAGTC 3 cut(s) 60, 137, 640
ScrFI CCNGG 4 cut(s) 375, 485, 553, 731
SduI GDGCHC 2 cut(s) 492, 870
SexAI ACCWGGT 1 cut(s) 729
SfaNI GCATC 1 cut(s) 723
SfcI CTRYAG 1 cut(s) 629
SinI GGWCC 3 cut(s) 190, 555, 947
SmiMI CAYNNNNRTG 2 cut(s) 176, 702
SmlI CTYRAG 2 cut(s) 224, 932
SmoI CTYRAG 2 cut(s) 224, 932
SpeI ACTAGT 1 cut(s) 676
Sse9I AATT 9 cut(s) 68, 106, 258, 317, 536, 756, 906, 942, 1010
SseBI AGGCCT 2 cut(s) 694, 750
SsiI CCGC 2 cut(s) 101, 957
SspMI CTAG 2 cut(s) 677, 830
SstI GAGCTC 1 cut(s) 492
StuI AGGCCT 2 cut(s) 694, 750
StyD4I CCNGG 4 cut(s) 373, 483, 551, 729
StyI CCWWGG 5 cut(s) 277, 342, 516, 796, 1056
TaaI ACNGT 3 cut(s) 186, 400, 880
TaqI TCGA 4 cut(s) 93, 426, 509, 864
TasI AATT 9 cut(s) 68, 106, 258, 317, 536, 756, 906, 942, 1010
TatI WGTACW 1 cut(s) 1023
TfiI GAWTC 2 cut(s) 83, 835
Tru1I TTAA 3 cut(s) 273, 759, 1041
Tru9I TTAA 3 cut(s) 273, 759, 1041
TscAI CASTG 2 cut(s) 93, 642
TseFI GTSAC 4 cut(s) 73, 633, 874, 952
Tsp45I GTSAC 4 cut(s) 73, 633, 874, 952
TspDTI ATGAA 5 cut(s) 234, 436, 605, 909, 910
TspRI CASTG 2 cut(s) 93, 642
Van91I CCANNNNNTGG 1 cut(s) 989
VpaK11BI GGWCC 3 cut(s) 190, 555, 947
XapI RAATTY 3 cut(s) 68, 258, 1010
XbaI TCTAGA 1 cut(s) 829
XceI RCATGY 2 cut(s) 246, 532
XcmI CCANNNNNNNNNTGG 1 cut(s) 734
XspI CTAG 2 cut(s) 677, 830
ZrmI AGTACT 1 cut(s) 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.