FvH4_6g32471

Poly(A) polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000312
Physical Location & Seq
Reverse (-)
25522168 .. 25527826
5659 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32471.t27

Sequence Viewer

Length: 1239 bp
ATGGTGTGCTCGGAGAGATTGAGTGCCACGCTGCCGCCGCCGCAGGCTGTGGTTGCCAAGCAATTCGGGGTCACGAAGCCGATTTCGATGGCCGGGCCCACAGAGGCCGATACTCAGAGGACTCTTGAGTTGGAGAAGATGTTGGTTGAAGCTGGGCTGTATGAAAGTAAAGAGGAAGCTGCCAAGAGAGAGGAGGTTCTTCGACGAATCGGGCAGATTGTTAATGACTGGGTGAAGCACCTTACTCAGTTGAGAGGATACACGGATCAAATGGTTGAGGATGCTAATGCTCTTATTTTTACATTTGGTTCCTATAGGCTTGGGGTACACGGTCCTGGGGCCGACATAGACACATTGTGTGTTGGGCCATGTTATGTTAACCGTGAGGAAGACTTCTTCTTTGTATTGCACAACATCCTGGCTGAAATGGAAGAAGTGACTGAACTGCAACCAGTTCCAGATGCTCATGTTCCTGTAATGAAGTTTAAGTTCGATGGAATATCAATCGACCTCCTTTATGCAAGTGTTTCTATGTTAGTTGTACCAGAAGACCTGGACATTTCTGATGTATCTCTGTTGTGCAACATCGATGAACCTACTGTTCGAAGTCTTAATGGCTGCAGGGTTGCTGATCAAATACTTAAGCTTGTTCCAAATGTTGAGCATTTCTGTACAACACTTCGTTGCTTGAAGTTTTGGGCGAAAAGGCGTGGTGTCTATTCCAATGTTACTGGATTTCTTGGTGGTGTGAACTGGGCTCTTCTCGTTGCCCGGGTGTGTCAACTTTATCCTAATGCAGTTCCCAGCATGCTGGTTTCTCGATTTTTTAGAGTCTACACACAGTGGCGATGGCCAAATCCTGTCATGTTATGTGCCATTGAAGAGGATGAACTTGGTTTAATTGTTTGGGACCCACGCAAGAATCCTCGTGACCGGACTCATCATATGCCAATTATAACTCCTGCATACCCTTGTATGAACTCTAGTTACAATGTTTCTAAAAGTACCCTCCGTGTCATGATGGAGCAGTTTCAGCATGGGAACAGGAAATGTGAGGAAATAGAACTGAATAAAGCTAGGTGGTCTGCTCTATTCAAACCGTATTTGTTCTTTGAAAGCTACAAGAACTATCTCCAGGTCGACATTGTTGCAGTTGATGTTGATGATCTGCGTGCCTGGAAAGGCTGGGTGGAATCTCGGTTGAGGCAATTGACTCTAATGGTAATAAGAACATATTAG

Protein Analysis

413

Amino Acids

47.19

Weight (kDa)

5.85

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_NTPase PF20750 23 - 217 2.8e-77 Poly(A) polymerase nucleotidyltransferase domain
NTP_transf_2 PF01909 83 - 172 2.9e-11 Nucleotidyltransferase domain
PAP_central PF04928 222 - 366 1.8e-68 Poly(A) polymerase central domain
PAP_RNA-bind PF04926 370 - 405 1.7e-07 Poly(A) polymerase predicted RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000312)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850
fragaria_vesca FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471
malus_domestica MD08G1110400.v1.1 MD09G1202000.v1.1 MD09G1202100.v1.1 MD17G1182800.v1.1
prunus_persica Prupe.1G444600_v2.0.a1 Prupe.3G057600_v2.0.a1 Prupe.3G057600_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1
pyrus_communis pycom08g09190 pycom09g11890 pycom15g08420 pycom17g19200
rosa_chinensis RchiOBHm_Chr2g0140721 RchiOBHm_Chr2g0140741 RchiOBHm_Chr2g0140761 RchiOBHm_Chr6g0306691
rosa_laevigata RLG00000010773 RLG00000019895 RLG00000019896
rosa_multiflora Rmu_co8193342.1_g000001 Rmu_sc0003342.1_g000015 Rmu_sc0003342.1_g000021 Rmu_sc0003342.1_g000026 Rmu_sc0005201.1_g000002 Rmu_sc0017785.1_g000009
rosa_roxburghii Rroxscaffold_2G00104510 Rroxscaffold_2G00104540 Rroxscaffold_7G00161540
rosa_rugosa Rorug02G0364600 Rorug02G0364700 Rorug02G0364800 Rorug02G0364900 Rorug02G0365000 Rorug06G0351600 Rorug06G0351700
rosa_samantha Rh2AG416100 Rh2AG416200 Rh2AG416300 Rh2BG425700 Rh2BG425800 Rh2BG425900 Rh2CG402300 Rh2CG402400 Rh2CG402500 Rh2DG435600 Rh2DG435700 Rh2DG435800 Rh6AG464000 Rh6BG437000 Rh6CG478100 Rh6DG464900
rosa_wichuraiana Rw2G034050 Rw2G034060 Rw6G040420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 956
AccI GTMKAC 2 cut(s) 834, 1140
AciI CCGC 3 cut(s) 35, 38, 41
AclWI GGATC 1 cut(s) 273
AcoI YGGCCR 2 cut(s) 90, 851
AdeI CACNNNGTG 2 cut(s) 357, 843
AfaI GTAC 4 cut(s) 327, 543, 673, 1006
AflII CTTAAG 1 cut(s) 641
AgsI TTSAA 5 cut(s) 149, 691, 881, 1096, 1115
AjnI CCWGG 5 cut(s) 334, 417, 552, 1134, 1175
AluBI AGCT 5 cut(s) 152, 179, 646, 1076, 1119
AluI AGCT 5 cut(s) 152, 179, 646, 1076, 1119
Alw21I GWGCWC 1 cut(s) 11
AlwI GGATC 1 cut(s) 273
Ama87I CYCGRG 1 cut(s) 771
AoxI GGCC 6 cut(s) 90, 95, 105, 339, 365, 851
ApaI GGGCCC 1 cut(s) 99
ApeKI GCWGC 3 cut(s) 31, 179, 618
ArsI GACNNNNNNTTYG 2 cut(s) 383, 415
AspS9I GGNCC 6 cut(s) 95, 96, 332, 339, 365, 910
AsuC2I CCSGG 3 cut(s) 94, 772, 773
AsuHPI GGTGA 1 cut(s) 244
AsuII TTCGAA 1 cut(s) 604
AvaI CYCGRG 1 cut(s) 771
AvaII GGWCC 2 cut(s) 332, 910
BaeGI GKGCMC 1 cut(s) 99
BalI TGGCCA 1 cut(s) 853
BanII GRGCYC 2 cut(s) 99, 760
BauI CACGAG 1 cut(s) 927
BbsI GAAGAC 2 cut(s) 396, 555
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 3 cut(s) 18, 166, 605
BccI CCATC 4 cut(s) 82, 488, 843, 1015
BcgI CGANNNNNNTGC 2 cut(s) 1130, 1164
BciT130I CCWGG 5 cut(s) 336, 419, 554, 1136, 1177
BciVI GTATCC 1 cut(s) 251
BclI TGATCA 1 cut(s) 631
BcnI CCSGG 3 cut(s) 94, 772, 773
BfaI CTAG 2 cut(s) 984, 1077
BfmI CTRYAG 2 cut(s) 313, 619
BfrI CTTAAG 1 cut(s) 641
BfuI GTATCC 1 cut(s) 251
BisI GCNGC 6 cut(s) 32, 35, 38, 41, 180, 619
BlsI GCNGC 6 cut(s) 33, 36, 39, 42, 181, 620
Bme1390I CCNGG 8 cut(s) 94, 336, 419, 554, 772, 773, 1136, 1177
Bme18I GGWCC 2 cut(s) 332, 910
BmeT110I CYCGRG 1 cut(s) 771
BmgT120I GGNCC 6 cut(s) 95, 96, 332, 339, 365, 910
BmiI GGNNCC 5 cut(s) 97, 310, 340, 911, 912
BmrFI CCNGG 8 cut(s) 94, 336, 419, 554, 772, 773, 1136, 1177
BmrI ACTGGG 2 cut(s) 238, 763
BmsI GCATC 2 cut(s) 271, 451
BmuI ACTGGG 2 cut(s) 238, 763
BpiI GAAGAC 2 cut(s) 396, 555
BpmI CTGGAG 1 cut(s) 1118
Bpu14I TTCGAA 1 cut(s) 604
BpuEI CTTGAG 1 cut(s) 146
BpuMI CCSGG 3 cut(s) 94, 772, 773
Bsa29I ATCGAT 1 cut(s) 588
BsaJI CCNNGG 2 cut(s) 335, 771
BsaWI WCCGGW 1 cut(s) 933
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bse1I ACTGG 4 cut(s) 233, 452, 736, 758
BseBI CCWGG 5 cut(s) 336, 419, 554, 1136, 1177
BseCI ATCGAT 1 cut(s) 588
BseDI CCNNGG 2 cut(s) 335, 771
BseGI GGATG 3 cut(s) 286, 414, 892
BseMII CTCAG 2 cut(s) 128, 260
BseNI ACTGG 4 cut(s) 233, 452, 736, 758
BseRI GAGGAG 1 cut(s) 206
BseSI GKGCMC 1 cut(s) 99
BseXI GCAGC 3 cut(s) 18, 166, 605
BseYI CCCAGC 3 cut(s) 152, 803, 1185
BshFI GGCC 6 cut(s) 92, 97, 107, 341, 367, 853
BshVI ATCGAT 1 cut(s) 588
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 1 cut(s) 771
BsiSI CCGG 3 cut(s) 93, 772, 934
BslFI GGGAC 1 cut(s) 923
BsmFI GGGAC 1 cut(s) 923
BsnI GGCC 6 cut(s) 92, 97, 107, 341, 367, 853
BsoBI CYCGRG 1 cut(s) 771
Bsp119I TTCGAA 1 cut(s) 604
Bsp120I GGGCCC 1 cut(s) 95
Bsp1286I GDGCHC 3 cut(s) 11, 99, 760
Bsp1407I TGTACA 1 cut(s) 671
Bsp143I GATC 3 cut(s) 265, 631, 1165
BspACI CCGC 3 cut(s) 35, 38, 41
BspANI GGCC 6 cut(s) 92, 97, 107, 341, 367, 853
BspCNI CTCAG 2 cut(s) 127, 259
BspDI ATCGAT 1 cut(s) 588
BspHI TCATGA 1 cut(s) 1017
BspLI GGNNCC 5 cut(s) 97, 310, 340, 911, 912
BspMAI CTGCAG 1 cut(s) 623
BspPI GGATC 1 cut(s) 273
BspQI GCTCTTC 1 cut(s) 765
BspT104I TTCGAA 1 cut(s) 604
BspTI CTTAAG 1 cut(s) 641
BsrGI TGTACA 1 cut(s) 671
BsrI ACTGG 4 cut(s) 233, 452, 736, 758
BssECI CCNNGG 2 cut(s) 335, 771
BssMI GATC 3 cut(s) 265, 631, 1165
BssSI CACGAG 1 cut(s) 927
Bst2BI CACGAG 1 cut(s) 927
Bst2UI CCWGG 5 cut(s) 336, 419, 554, 1136, 1177
Bst4CI ACNGT 5 cut(s) 332, 383, 601, 843, 1101
Bst6I CTCTTC 2 cut(s) 765, 876
BstAFI CTTAAG 1 cut(s) 641
BstAUI TGTACA 1 cut(s) 671
BstBI TTCGAA 1 cut(s) 604
BstC8I GCNNGC 3 cut(s) 45, 809, 1173
BstDEI CTNAG 2 cut(s) 114, 246
BstF5I GGATG 3 cut(s) 286, 414, 892
BstKTI GATC 3 cut(s) 268, 634, 1168
BstMBI GATC 3 cut(s) 265, 631, 1165
BstMWI GCNNNNNNNGC 4 cut(s) 37, 40, 53, 1033
BstNI CCWGG 5 cut(s) 336, 419, 554, 1136, 1177
BstNSI RCATGY 1 cut(s) 811
BstSCI CCNGG 8 cut(s) 92, 334, 417, 552, 770, 771, 1134, 1175
BstSFI CTRYAG 2 cut(s) 313, 619
BstSLI GKGCMC 1 cut(s) 99
BstV1I GCAGC 3 cut(s) 18, 166, 605
BstV2I GAAGAC 2 cut(s) 396, 555
BstXI CCANNNNNNTGG 1 cut(s) 811
Bsu15I ATCGAT 1 cut(s) 588
BsuI GTATCC 1 cut(s) 251
BsuRI GGCC 6 cut(s) 92, 97, 107, 341, 367, 853
BsuTUI ATCGAT 1 cut(s) 588
BtgZI GCGATG 1 cut(s) 862
BtsCI GGATG 3 cut(s) 286, 414, 892
BtsIMutI CAGTG 1 cut(s) 848
Cac8I GCNNGC 3 cut(s) 45, 809, 1173
CciI TCATGA 1 cut(s) 1017
Cfr13I GGNCC 6 cut(s) 95, 96, 332, 339, 365, 910
Cfr9I CCCGGG 1 cut(s) 771
ClaI ATCGAT 1 cut(s) 588
Csp6I GTAC 4 cut(s) 326, 542, 672, 1005
CviAII CATG 6 cut(s) 369, 467, 808, 865, 1018, 1037
CviQI GTAC 4 cut(s) 326, 542, 672, 1005
DdeI CTNAG 2 cut(s) 114, 246
DpnI GATC 3 cut(s) 267, 633, 1167
DpnII GATC 3 cut(s) 265, 631, 1165
DraIII CACNNNGTG 2 cut(s) 357, 843
EaeI YGGCCR 2 cut(s) 90, 851
Eam1104I CTCTTC 2 cut(s) 765, 876
EarI CTCTTC 2 cut(s) 765, 876
Eco24I GRGCYC 2 cut(s) 99, 760
Eco47I GGWCC 2 cut(s) 332, 910
Eco88I CYCGRG 1 cut(s) 771
EcoO109I RGGNCCY 1 cut(s) 910
EcoRII CCWGG 5 cut(s) 334, 417, 552, 1134, 1175
EcoT38I GRGCYC 2 cut(s) 99, 760
FaeI CATG 6 cut(s) 372, 470, 811, 868, 1021, 1040
FaqI GGGAC 1 cut(s) 923
FatI CATG 6 cut(s) 368, 466, 807, 864, 1017, 1036
FauNDI CATATG 1 cut(s) 945
FbaI TGATCA 1 cut(s) 631
FblI GTMKAC 2 cut(s) 834, 1140
Fnu4HI GCNGC 6 cut(s) 32, 35, 38, 41, 180, 619
FokI GGATG 3 cut(s) 293, 401, 899
FriOI GRGCYC 2 cut(s) 99, 760
Fsp4HI GCNGC 6 cut(s) 32, 35, 38, 41, 180, 619
FspBI CTAG 2 cut(s) 984, 1077
GluI GCNGC 6 cut(s) 32, 35, 38, 41, 180, 619
GsaI CCCAGC 3 cut(s) 156, 807, 1189
GsuI CTGGAG 1 cut(s) 1118
HaeIII GGCC 6 cut(s) 92, 97, 107, 341, 367, 853
HapII CCGG 3 cut(s) 93, 772, 934
Hin1II CATG 6 cut(s) 372, 470, 811, 868, 1021, 1040
HincII GTYRAC 3 cut(s) 379, 782, 1141
HindII GTYRAC 3 cut(s) 379, 782, 1141
HindIII AAGCTT 1 cut(s) 644
HinfI GANTC 7 cut(s) 121, 207, 831, 922, 937, 1193, 1213
HpaI GTTAAC 1 cut(s) 379
HpaII CCGG 3 cut(s) 93, 772, 934
HphI GGTGA 1 cut(s) 244
Hpy166II GTNNAC 6 cut(s) 328, 379, 751, 782, 835, 1141
Hpy188I TCNGA 3 cut(s) 13, 117, 565
Hpy188III TCNNGA 6 cut(s) 73, 125, 458, 819, 929, 1018
Hpy8I GTNNAC 6 cut(s) 328, 379, 751, 782, 835, 1141
Hpy99I CGWCG 1 cut(s) 207
HpyCH4III ACNGT 5 cut(s) 332, 383, 601, 843, 1101
HpyCH4V TGCA 8 cut(s) 409, 448, 521, 582, 621, 797, 965, 1151
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 40, 53, 1033
HpyF3I CTNAG 2 cut(s) 114, 246
Hsp92II CATG 6 cut(s) 372, 470, 811, 868, 1021, 1040
KflI GGGWCCC 1 cut(s) 910
Ksp22I TGATCA 1 cut(s) 631
KspAI GTTAAC 1 cut(s) 379
Kzo9I GATC 3 cut(s) 265, 631, 1165
LguI GCTCTTC 1 cut(s) 765
LmnI GCTCC 1 cut(s) 1024
Lsp1109I GCAGC 3 cut(s) 18, 166, 605
LweI GCATC 2 cut(s) 271, 451
MaeI CTAG 2 cut(s) 984, 1077
MaeIII GTNAC 5 cut(s) 70, 436, 727, 929, 986
MalI GATC 3 cut(s) 267, 633, 1167
MboI GATC 3 cut(s) 265, 631, 1165
MboII GAAGA 8 cut(s) 148, 191, 388, 401, 443, 560, 752, 893
MfeI CAATTG 1 cut(s) 1208
MhlI GDGCHC 3 cut(s) 11, 99, 760
MlsI TGGCCA 1 cut(s) 853
MluCI AATT 4 cut(s) 62, 900, 951, 1208
MluNI TGGCCA 1 cut(s) 853
MlyI GAGTC 4 cut(s) 115, 840, 931, 1207
MmeI TCCRAC 1 cut(s) 111
Mox20I TGGCCA 1 cut(s) 853
MscI TGGCCA 1 cut(s) 853
MseI TTAA 6 cut(s) 222, 378, 486, 612, 642, 899
Msp20I TGGCCA 1 cut(s) 853
MspCI CTTAAG 1 cut(s) 641
MspI CCGG 3 cut(s) 93, 772, 934
MspR9I CCNGG 8 cut(s) 94, 336, 419, 554, 772, 773, 1136, 1177
MunI CAATTG 1 cut(s) 1208
MvaI CCWGG 5 cut(s) 336, 419, 554, 1136, 1177
MwoI GCNNNNNNNGC 4 cut(s) 37, 40, 53, 1033
NciI CCSGG 3 cut(s) 94, 772, 773
NdeI CATATG 1 cut(s) 945
NdeII GATC 3 cut(s) 265, 631, 1165
NlaIII CATG 6 cut(s) 372, 470, 811, 868, 1021, 1040
NlaIV GGNNCC 5 cut(s) 97, 310, 340, 911, 912
NmuCI GTSAC 3 cut(s) 70, 436, 929
NspI RCATGY 1 cut(s) 811
NspV TTCGAA 1 cut(s) 604
PaeI GCATGC 1 cut(s) 811
PagI TCATGA 1 cut(s) 1017
PciSI GCTCTTC 1 cut(s) 765
PfeI GAWTC 3 cut(s) 207, 922, 1193
PkrI GCNGC 6 cut(s) 33, 36, 39, 42, 181, 620
PleI GAGTC 4 cut(s) 115, 839, 931, 1207
PpsI GAGTC 4 cut(s) 115, 839, 931, 1207
PpuMI RGGWCCY 1 cut(s) 910
PsiI TTATAA 1 cut(s) 956
Psp5II RGGWCCY 1 cut(s) 910
Psp6I CCWGG 5 cut(s) 334, 417, 552, 1134, 1175
PspFI CCCAGC 3 cut(s) 152, 803, 1185
PspGI CCWGG 5 cut(s) 334, 417, 552, 1134, 1175
PspN4I GGNNCC 5 cut(s) 97, 310, 340, 911, 912
PspOMI GGGCCC 1 cut(s) 95
PspPI GGNCC 6 cut(s) 95, 96, 332, 339, 365, 910
PspPPI RGGWCCY 1 cut(s) 910
PsrI GAACNNNNNNTAC 2 cut(s) 971, 1003
PstI CTGCAG 1 cut(s) 623
RsaI GTAC 4 cut(s) 327, 543, 673, 1006
RsaNI GTAC 4 cut(s) 326, 542, 672, 1005
SalI GTCGAC 1 cut(s) 1139
SapI GCTCTTC 1 cut(s) 765
SaqAI TTAA 6 cut(s) 222, 378, 486, 612, 642, 899
SatI GCNGC 6 cut(s) 32, 35, 38, 41, 180, 619
Sau3AI GATC 3 cut(s) 265, 631, 1165
Sau96I GGNCC 6 cut(s) 95, 96, 332, 339, 365, 910
SchI GAGTC 4 cut(s) 115, 840, 931, 1207
ScrFI CCNGG 8 cut(s) 94, 336, 419, 554, 772, 773, 1136, 1177
SduI GDGCHC 3 cut(s) 11, 99, 760
SfaNI GCATC 2 cut(s) 271, 451
SfcI CTRYAG 2 cut(s) 313, 619
SfuI TTCGAA 1 cut(s) 604
SinI GGWCC 2 cut(s) 332, 910
SmaI CCCGGG 1 cut(s) 773
SmlI CTYRAG 2 cut(s) 125, 641
SmoI CTYRAG 2 cut(s) 125, 641
SphI GCATGC 1 cut(s) 811
Sse9I AATT 4 cut(s) 62, 900, 951, 1208
SsiI CCGC 3 cut(s) 35, 38, 41
SspMI CTAG 2 cut(s) 984, 1077
StyD4I CCNGG 8 cut(s) 92, 334, 417, 552, 770, 771, 1134, 1175
TaaI ACNGT 5 cut(s) 332, 383, 601, 843, 1101
TaqI TCGA 8 cut(s) 86, 202, 492, 507, 588, 604, 820, 1140
TasI AATT 4 cut(s) 62, 900, 951, 1208
TatI WGTACW 1 cut(s) 671
TauI GCSGC 3 cut(s) 37, 40, 43
TfiI GAWTC 3 cut(s) 207, 922, 1193
Tru1I TTAA 6 cut(s) 222, 378, 486, 612, 642, 899
Tru9I TTAA 6 cut(s) 222, 378, 486, 612, 642, 899
TscAI CASTG 1 cut(s) 848
TseFI GTSAC 3 cut(s) 70, 436, 929
TseI GCWGC 3 cut(s) 31, 179, 618
Tsp45I GTSAC 3 cut(s) 70, 436, 929
TspDTI ATGAA 5 cut(s) 177, 494, 606, 903, 992
TspGWI ACGGA 2 cut(s) 278, 1001
TspMI CCCGGG 1 cut(s) 771
TspRI CASTG 1 cut(s) 848
Vha464I CTTAAG 1 cut(s) 641
VpaK11BI GGWCC 2 cut(s) 332, 910
XceI RCATGY 1 cut(s) 811
XmaI CCCGGG 1 cut(s) 771
XmiI GTMKAC 2 cut(s) 834, 1140
XspI CTAG 2 cut(s) 984, 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.