Poly(A) polymerase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| PAP_NTPase | PF20750 | 25 - 219 | 1.1e-72 | Poly(A) polymerase nucleotidyltransferase domain |
| NTP_transf_2 | PF01909 | 85 - 174 | 1.8e-08 | Nucleotidyltransferase domain |
| PAP_central | PF04928 | 224 - 368 | 1.7e-68 | Poly(A) polymerase central domain |
| PAP_RNA-bind | PF04926 | 372 - 430 | 2.5e-10 | Poly(A) polymerase predicted RNA binding domain |
| PAP_RNA-bind | PF04926 | 429 - 503 | 9e-07 | Poly(A) polymerase predicted RNA binding domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 962 |
| Acc36I | ACCTGC | 1 cut(s) | 1633 |
| AccBSI | CCGCTC | 1 cut(s) | 1234 |
| AccI | GTMKAC | 2 cut(s) | 840, 1146 |
| AccII | CGCG | 1 cut(s) | 936 |
| AciI | CCGC | 2 cut(s) | 98, 1234 |
| AclI | AACGTT | 2 cut(s) | 1718, 1773 |
| AcoI | YGGCCR | 3 cut(s) | 561, 857, 1527 |
| AcsI | RAATTY | 4 cut(s) | 642, 1440, 1753, 1802 |
| AdeI | CACNNNGTG | 3 cut(s) | 363, 849, 1310 |
| AfaI | GTAC | 7 cut(s) | 333, 549, 587, 679, 1012, 1415, 1657 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 347, 1745 |
| AflII | CTTAAG | 1 cut(s) | 647 |
| AflIII | ACRYGT | 1 cut(s) | 1275 |
| AgsI | TTSAA | 4 cut(s) | 155, 887, 1120, 1703 |
| AhlI | ACTAGT | 2 cut(s) | 527, 1787 |
| AjnI | CCWGG | 5 cut(s) | 340, 558, 1140, 1429, 1738 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 888, 920 |
| AluBI | AGCT | 8 cut(s) | 15, 158, 185, 652, 993, 1082, 1125, 1399 |
| AluI | AGCT | 8 cut(s) | 15, 158, 185, 652, 993, 1082, 1125, 1399 |
| Alw21I | GWGCWC | 3 cut(s) | 17, 1096, 1306 |
| Alw26I | GTCTC | 2 cut(s) | 253, 1567 |
| Alw44I | GTGCAC | 1 cut(s) | 1302 |
| Ama87I | CYCGRG | 1 cut(s) | 777 |
| AoxI | GGCC | 6 cut(s) | 51, 101, 371, 561, 857, 1527 |
| ApaLI | GTGCAC | 1 cut(s) | 1302 |
| ApeKI | GCWGC | 3 cut(s) | 185, 624, 1534 |
| ApoI | RAATTY | 4 cut(s) | 642, 1440, 1753, 1802 |
| AseI | ATTAAT | 1 cut(s) | 1407 |
| AspS9I | GGNCC | 4 cut(s) | 101, 338, 371, 916 |
| AsuHPI | GGTGA | 3 cut(s) | 200, 250, 1580 |
| AsuII | TTCGAA | 3 cut(s) | 610, 1101, 1797 |
| AvaI | CYCGRG | 1 cut(s) | 777 |
| AvaII | GGWCC | 2 cut(s) | 338, 916 |
| BaeGI | GKGCMC | 1 cut(s) | 1306 |
| BalI | TGGCCA | 3 cut(s) | 563, 859, 1529 |
| BanII | GRGCYC | 2 cut(s) | 17, 766 |
| BbsI | GAAGAC | 2 cut(s) | 402, 561 |
| Bbv12I | GWGCWC | 3 cut(s) | 17, 1096, 1306 |
| BbvI | GCAGC | 3 cut(s) | 172, 611, 1546 |
| BccI | CCATC | 8 cut(s) | 382, 494, 849, 1021, 1429, 1487, 1640, 1739 |
| BceAI | ACGGC | 1 cut(s) | 38 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 233, 267, 1136, 1170 |
| BciT130I | CCWGG | 5 cut(s) | 342, 560, 1142, 1431, 1740 |
| BciVI | GTATCC | 2 cut(s) | 1231, 1278 |
| BclI | TGATCA | 2 cut(s) | 637, 1540 |
| BcoDI | GTCTC | 2 cut(s) | 253, 1567 |
| BcuI | ACTAGT | 2 cut(s) | 527, 1787 |
| BfaI | CTAG | 5 cut(s) | 528, 810, 990, 1779, 1788 |
| BfmI | CTRYAG | 1 cut(s) | 625 |
| BfrI | CTTAAG | 1 cut(s) | 647 |
| BfuAI | ACCTGC | 1 cut(s) | 1633 |
| BfuI | GTATCC | 2 cut(s) | 1231, 1278 |
| BisI | GCNGC | 3 cut(s) | 186, 625, 1535 |
| BlsI | GCNGC | 3 cut(s) | 187, 626, 1536 |
| Bme1390I | CCNGG | 5 cut(s) | 342, 560, 1142, 1431, 1740 |
| Bme18I | GGWCC | 2 cut(s) | 338, 916 |
| BmeT110I | CYCGRG | 1 cut(s) | 777 |
| BmgT120I | GGNCC | 4 cut(s) | 101, 338, 371, 916 |
| BmiI | GGNNCC | 4 cut(s) | 102, 917, 918, 1737 |
| BmrFI | CCNGG | 5 cut(s) | 342, 560, 1142, 1431, 1740 |
| BmrI | ACTGGG | 1 cut(s) | 769 |
| BmsI | GCATC | 5 cut(s) | 277, 457, 622, 680, 1637 |
| BmuI | ACTGGG | 1 cut(s) | 769 |
| BpiI | GAAGAC | 2 cut(s) | 402, 561 |
| BpmI | CTGGAG | 5 cut(s) | 1124, 1647, 1746, 1754, 1761 |
| Bpu14I | TTCGAA | 3 cut(s) | 610, 1101, 1797 |
| BpuEI | CTTGAG | 1 cut(s) | 715 |
| Bsa29I | ATCGAT | 1 cut(s) | 594 |
| BsaAI | YACGTR | 1 cut(s) | 1278 |
| BsaBI | GATNNNNATC | 1 cut(s) | 636 |
| BsaJI | CCNNGG | 3 cut(s) | 54, 341, 1430 |
| BsaWI | WCCGGW | 1 cut(s) | 939 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 347, 1745 |
| Bse1I | ACTGG | 4 cut(s) | 458, 764, 1077, 1737 |
| Bse8I | GATNNNNATC | 1 cut(s) | 636 |
| BseBI | CCWGG | 5 cut(s) | 342, 560, 1142, 1431, 1740 |
| BseCI | ATCGAT | 1 cut(s) | 594 |
| BseDI | CCNNGG | 3 cut(s) | 54, 341, 1430 |
| BseGI | GGATG | 5 cut(s) | 292, 637, 898, 1440, 1628 |
| BseJI | GATNNNNATC | 1 cut(s) | 636 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 347, 1745 |
| BseMII | CTCAG | 2 cut(s) | 30, 1050 |
| BseNI | ACTGG | 4 cut(s) | 458, 764, 1077, 1737 |
| BseRI | GAGGAG | 2 cut(s) | 212, 758 |
| BseSI | GKGCMC | 1 cut(s) | 1306 |
| BseXI | GCAGC | 3 cut(s) | 172, 611, 1546 |
| BseYI | CCCAGC | 2 cut(s) | 158, 1191 |
| Bsh1236I | CGCG | 1 cut(s) | 936 |
| Bsh1285I | CGRYCG | 1 cut(s) | 940 |
| BshFI | GGCC | 6 cut(s) | 53, 103, 373, 563, 859, 1529 |
| BshVI | ATCGAT | 1 cut(s) | 594 |
| BsiEI | CGRYCG | 1 cut(s) | 940 |
| BsiHKAI | GWGCWC | 3 cut(s) | 17, 1096, 1306 |
| BsiHKCI | CYCGRG | 1 cut(s) | 777 |
| BsiSI | CCGG | 2 cut(s) | 940, 1613 |
| BslFI | GGGAC | 3 cut(s) | 359, 929, 1393 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 347, 1745 |
| BsmAI | GTCTC | 2 cut(s) | 253, 1567 |
| BsmBI | CGTCTC | 1 cut(s) | 253 |
| BsmFI | GGGAC | 3 cut(s) | 359, 929, 1393 |
| BsnI | GGCC | 6 cut(s) | 53, 103, 373, 563, 859, 1529 |
| BsoBI | CYCGRG | 1 cut(s) | 777 |
| Bsp119I | TTCGAA | 3 cut(s) | 610, 1101, 1797 |
| Bsp1286I | GDGCHC | 4 cut(s) | 17, 766, 1096, 1306 |
| Bsp1407I | TGTACA | 3 cut(s) | 585, 677, 1413 |
| Bsp143I | GATC | 5 cut(s) | 271, 637, 1171, 1540, 1594 |
| Bsp68I | TCGCGA | 1 cut(s) | 936 |
| BspACI | CCGC | 2 cut(s) | 98, 1234 |
| BspANI | GGCC | 6 cut(s) | 53, 103, 373, 563, 859, 1529 |
| BspCNI | CTCAG | 2 cut(s) | 29, 1051 |
| BspDI | ATCGAT | 1 cut(s) | 594 |
| BspFNI | CGCG | 1 cut(s) | 936 |
| BspHI | TCATGA | 2 cut(s) | 1023, 1543 |
| BspLI | GGNNCC | 4 cut(s) | 102, 917, 918, 1737 |
| BspMAI | CTGCAG | 1 cut(s) | 629 |
| BspMI | ACCTGC | 1 cut(s) | 1633 |
| BspT104I | TTCGAA | 3 cut(s) | 610, 1101, 1797 |
| BspTI | CTTAAG | 1 cut(s) | 647 |
| BsrBI | CCGCTC | 1 cut(s) | 1234 |
| BsrGI | TGTACA | 3 cut(s) | 585, 677, 1413 |
| BsrI | ACTGG | 4 cut(s) | 458, 764, 1077, 1737 |
| BssECI | CCNNGG | 3 cut(s) | 54, 341, 1430 |
| BssMI | GATC | 5 cut(s) | 271, 637, 1171, 1540, 1594 |
| Bst2UI | CCWGG | 5 cut(s) | 342, 560, 1142, 1431, 1740 |
| Bst4CI | ACNGT | 7 cut(s) | 389, 607, 849, 1299, 1310, 1384, 1515 |
| Bst6I | CTCTTC | 3 cut(s) | 171, 882, 1583 |
| BstAFI | CTTAAG | 1 cut(s) | 647 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1310 |
| BstAUI | TGTACA | 3 cut(s) | 585, 677, 1413 |
| BstBAI | YACGTR | 1 cut(s) | 1278 |
| BstBI | TTCGAA | 3 cut(s) | 610, 1101, 1797 |
| BstC8I | GCNNGC | 4 cut(s) | 6, 51, 815, 1179 |
| BstDEI | CTNAG | 3 cut(s) | 16, 1059, 1083 |
| BstDSI | CCRYGG | 1 cut(s) | 54 |
| BstF5I | GGATG | 5 cut(s) | 292, 637, 898, 1440, 1628 |
| BstFNI | CGCG | 1 cut(s) | 936 |
| BstKTI | GATC | 5 cut(s) | 274, 640, 1174, 1543, 1597 |
| BstMAI | GTCTC | 2 cut(s) | 253, 1567 |
| BstMBI | GATC | 5 cut(s) | 271, 637, 1171, 1540, 1594 |
| BstMCI | CGRYCG | 1 cut(s) | 940 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1039, 1310, 1336 |
| BstNI | CCWGG | 5 cut(s) | 342, 560, 1142, 1431, 1740 |
| BstSCI | CCNGG | 5 cut(s) | 340, 558, 1140, 1429, 1738 |
| BstSFI | CTRYAG | 1 cut(s) | 625 |
| BstSLI | GKGCMC | 1 cut(s) | 1306 |
| BstUI | CGCG | 1 cut(s) | 936 |
| BstV1I | GCAGC | 3 cut(s) | 172, 611, 1546 |
| BstV2I | GAAGAC | 2 cut(s) | 402, 561 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1525 |
| Bsu15I | ATCGAT | 1 cut(s) | 594 |
| BsuI | GTATCC | 2 cut(s) | 1231, 1278 |
| BsuRI | GGCC | 6 cut(s) | 53, 103, 373, 563, 859, 1529 |
| BsuTUI | ATCGAT | 1 cut(s) | 594 |
| BtgI | CCRYGG | 1 cut(s) | 54 |
| BtgZI | GCGATG | 2 cut(s) | 23, 868 |
| BtsCI | GGATG | 5 cut(s) | 292, 637, 898, 1440, 1628 |
| BtsIMutI | CAGTG | 3 cut(s) | 854, 1304, 1315 |
| BtuMI | TCGCGA | 1 cut(s) | 936 |
| BveI | ACCTGC | 1 cut(s) | 1633 |
| Cac8I | GCNNGC | 4 cut(s) | 6, 51, 815, 1179 |
| CciI | TCATGA | 2 cut(s) | 1023, 1543 |
| Cfr13I | GGNCC | 4 cut(s) | 101, 338, 371, 916 |
| ClaI | ATCGAT | 1 cut(s) | 594 |
| Csp6I | GTAC | 7 cut(s) | 332, 548, 586, 678, 1011, 1414, 1656 |
| CviAII | CATG | 8 cut(s) | 335, 871, 1024, 1043, 1454, 1538, 1544, 1783 |
| CviQI | GTAC | 7 cut(s) | 332, 548, 586, 678, 1011, 1414, 1656 |
| DdeI | CTNAG | 3 cut(s) | 16, 1059, 1083 |
| DpnI | GATC | 5 cut(s) | 273, 639, 1173, 1542, 1596 |
| DpnII | GATC | 5 cut(s) | 271, 637, 1171, 1540, 1594 |
| DraIII | CACNNNGTG | 3 cut(s) | 363, 849, 1310 |
| EaeI | YGGCCR | 3 cut(s) | 561, 857, 1527 |
| Eam1104I | CTCTTC | 3 cut(s) | 171, 882, 1583 |
| EarI | CTCTTC | 3 cut(s) | 171, 882, 1583 |
| Ecl136II | GAGCTC | 1 cut(s) | 15 |
| Eco24I | GRGCYC | 2 cut(s) | 17, 766 |
| Eco47I | GGWCC | 2 cut(s) | 338, 916 |
| Eco53kI | GAGCTC | 1 cut(s) | 15 |
| Eco88I | CYCGRG | 1 cut(s) | 777 |
| EcoICRI | GAGCTC | 1 cut(s) | 15 |
| EcoO109I | RGGNCCY | 1 cut(s) | 916 |
| EcoRII | CCWGG | 5 cut(s) | 340, 558, 1140, 1429, 1738 |
| EcoT38I | GRGCYC | 2 cut(s) | 17, 766 |
| Esp3I | CGTCTC | 1 cut(s) | 253 |
| FaeI | CATG | 8 cut(s) | 338, 874, 1027, 1046, 1457, 1541, 1547, 1786 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 131, 163, 147, 179 |
| FaqI | GGGAC | 3 cut(s) | 359, 929, 1393 |
| FatI | CATG | 8 cut(s) | 334, 870, 1023, 1042, 1453, 1537, 1543, 1782 |
| FauI | CCCGC | 2 cut(s) | 91, 1227 |
| FauNDI | CATATG | 1 cut(s) | 951 |
| FbaI | TGATCA | 2 cut(s) | 637, 1540 |
| FblI | GTMKAC | 2 cut(s) | 840, 1146 |
| Fnu4HI | GCNGC | 3 cut(s) | 186, 625, 1535 |
| FokI | GGATG | 5 cut(s) | 299, 644, 905, 1447, 1615 |
| FriOI | GRGCYC | 2 cut(s) | 17, 766 |
| Fsp4HI | GCNGC | 3 cut(s) | 186, 625, 1535 |
| FspBI | CTAG | 5 cut(s) | 528, 810, 990, 1779, 1788 |
| GluI | GCNGC | 3 cut(s) | 186, 625, 1535 |
| GsaI | CCCAGC | 2 cut(s) | 162, 1195 |
| GsuI | CTGGAG | 5 cut(s) | 1124, 1647, 1746, 1754, 1761 |
| HaeIII | GGCC | 6 cut(s) | 53, 103, 373, 563, 859, 1529 |
| HapII | CCGG | 2 cut(s) | 940, 1613 |
| Hin1II | CATG | 8 cut(s) | 338, 874, 1027, 1046, 1457, 1541, 1547, 1786 |
| HincII | GTYRAC | 4 cut(s) | 385, 788, 1147, 1162 |
| HindII | GTYRAC | 4 cut(s) | 385, 788, 1147, 1162 |
| HindIII | AAGCTT | 1 cut(s) | 650 |
| HinfI | GANTC | 6 cut(s) | 110, 837, 943, 1020, 1199, 1703 |
| HpaII | CCGG | 2 cut(s) | 940, 1613 |
| HphI | GGTGA | 3 cut(s) | 200, 250, 1580 |
| Hpy166II | GTNNAC | 8 cut(s) | 385, 757, 788, 841, 1147, 1162, 1304, 1502 |
| Hpy188I | TCNGA | 7 cut(s) | 19, 115, 123, 571, 689, 1019, 1060 |
| Hpy188III | TCNNGA | 9 cut(s) | 464, 825, 935, 1024, 1490, 1518, 1544, 1664, 1725 |
| Hpy8I | GTNNAC | 8 cut(s) | 385, 757, 788, 841, 1147, 1162, 1304, 1502 |
| HpyCH4III | ACNGT | 7 cut(s) | 389, 607, 849, 1299, 1310, 1384, 1515 |
| HpyCH4IV | ACGT | 4 cut(s) | 1164, 1277, 1718, 1773 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1039, 1310, 1336 |
| HpyF3I | CTNAG | 3 cut(s) | 16, 1059, 1083 |
| HpySE526I | ACGT | 4 cut(s) | 1164, 1277, 1718, 1773 |
| Hsp92II | CATG | 8 cut(s) | 338, 874, 1027, 1046, 1457, 1541, 1547, 1786 |
| KflI | GGGWCCC | 1 cut(s) | 916 |
| Ksp22I | TGATCA | 2 cut(s) | 637, 1540 |
| Kzo9I | GATC | 5 cut(s) | 271, 637, 1171, 1540, 1594 |
| LmnI | GCTCC | 2 cut(s) | 1030, 1735 |
| Lsp1109I | GCAGC | 3 cut(s) | 172, 611, 1546 |
| LweI | GCATC | 5 cut(s) | 277, 457, 622, 680, 1637 |
| MaeI | CTAG | 5 cut(s) | 528, 810, 990, 1779, 1788 |
| MaeII | ACGT | 4 cut(s) | 1164, 1277, 1718, 1773 |
| MaeIII | GTNAC | 5 cut(s) | 76, 442, 532, 1251, 1774 |
| MalI | GATC | 5 cut(s) | 273, 639, 1173, 1542, 1596 |
| MbiI | CCGCTC | 1 cut(s) | 1234 |
| MboI | GATC | 5 cut(s) | 271, 637, 1171, 1540, 1594 |
| MboII | GAAGA | 8 cut(s) | 188, 197, 407, 449, 566, 899, 1108, 1600 |
| MhlI | GDGCHC | 4 cut(s) | 17, 766, 1096, 1306 |
| MlsI | TGGCCA | 3 cut(s) | 563, 859, 1529 |
| MluCI | AATT | 8 cut(s) | 213, 642, 957, 1417, 1440, 1582, 1753, 1802 |
| MluNI | TGGCCA | 3 cut(s) | 563, 859, 1529 |
| MlyI | GAGTC | 3 cut(s) | 846, 937, 1029 |
| Mox20I | TGGCCA | 3 cut(s) | 563, 859, 1529 |
| MscI | TGGCCA | 3 cut(s) | 563, 859, 1529 |
| MseI | TTAA | 7 cut(s) | 137, 228, 492, 618, 648, 1395, 1407 |
| MslI | CAYNNNNRTG | 4 cut(s) | 688, 1278, 1409, 1542 |
| Msp20I | TGGCCA | 3 cut(s) | 563, 859, 1529 |
| MspA1I | CMGCKG | 1 cut(s) | 98 |
| MspCI | CTTAAG | 1 cut(s) | 647 |
| MspI | CCGG | 2 cut(s) | 940, 1613 |
| MspR9I | CCNGG | 5 cut(s) | 342, 560, 1142, 1431, 1740 |
| MvaI | CCWGG | 5 cut(s) | 342, 560, 1142, 1431, 1740 |
| MvnI | CGCG | 1 cut(s) | 936 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1039, 1310, 1336 |
| NdeI | CATATG | 1 cut(s) | 951 |
| NdeII | GATC | 5 cut(s) | 271, 637, 1171, 1540, 1594 |
| NlaIII | CATG | 8 cut(s) | 338, 874, 1027, 1046, 1457, 1541, 1547, 1786 |
| NlaIV | GGNNCC | 4 cut(s) | 102, 917, 918, 1737 |
| NmuCI | GTSAC | 2 cut(s) | 76, 442 |
| NruI | TCGCGA | 1 cut(s) | 936 |
| NspV | TTCGAA | 3 cut(s) | 610, 1101, 1797 |
| PagI | TCATGA | 2 cut(s) | 1023, 1543 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 259 |
| PfeI | GAWTC | 3 cut(s) | 110, 1199, 1703 |
| PkrI | GCNGC | 3 cut(s) | 187, 626, 1536 |
| PleI | GAGTC | 3 cut(s) | 845, 937, 1028 |
| PpsI | GAGTC | 3 cut(s) | 845, 937, 1028 |
| Ppu21I | YACGTR | 1 cut(s) | 1278 |
| PpuMI | RGGWCCY | 1 cut(s) | 916 |
| PshBI | ATTAAT | 1 cut(s) | 1407 |
| PsiI | TTATAA | 1 cut(s) | 962 |
| Psp124BI | GAGCTC | 1 cut(s) | 17 |
| Psp1406I | AACGTT | 2 cut(s) | 1718, 1773 |
| Psp5II | RGGWCCY | 1 cut(s) | 916 |
| Psp6I | CCWGG | 5 cut(s) | 340, 558, 1140, 1429, 1738 |
| PspFI | CCCAGC | 2 cut(s) | 158, 1191 |
| PspGI | CCWGG | 5 cut(s) | 340, 558, 1140, 1429, 1738 |
| PspN4I | GGNNCC | 4 cut(s) | 102, 917, 918, 1737 |
| PspPI | GGNCC | 4 cut(s) | 101, 338, 371, 916 |
| PspPPI | RGGWCCY | 1 cut(s) | 916 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 977, 1009 |
| PstI | CTGCAG | 1 cut(s) | 629 |
| RruI | TCGCGA | 1 cut(s) | 936 |
| RsaI | GTAC | 7 cut(s) | 333, 549, 587, 679, 1012, 1415, 1657 |
| RsaNI | GTAC | 7 cut(s) | 332, 548, 586, 678, 1011, 1414, 1656 |
| RseI | CAYNNNNRTG | 4 cut(s) | 688, 1278, 1409, 1542 |
| SacI | GAGCTC | 1 cut(s) | 17 |
| SalI | GTCGAC | 1 cut(s) | 1145 |
| SaqAI | TTAA | 7 cut(s) | 137, 228, 492, 618, 648, 1395, 1407 |
| SatI | GCNGC | 3 cut(s) | 186, 625, 1535 |
| Sau3AI | GATC | 5 cut(s) | 271, 637, 1171, 1540, 1594 |
| Sau96I | GGNCC | 4 cut(s) | 101, 338, 371, 916 |
| SchI | GAGTC | 3 cut(s) | 846, 937, 1029 |
| ScrFI | CCNGG | 5 cut(s) | 342, 560, 1142, 1431, 1740 |
| SduI | GDGCHC | 4 cut(s) | 17, 766, 1096, 1306 |
| SfaNI | GCATC | 5 cut(s) | 277, 457, 622, 680, 1637 |
| SfcI | CTRYAG | 1 cut(s) | 625 |
| SfuI | TTCGAA | 3 cut(s) | 610, 1101, 1797 |
| SinI | GGWCC | 2 cut(s) | 338, 916 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 688, 1278, 1409, 1542 |
| SmlI | CTYRAG | 2 cut(s) | 647, 694 |
| SmoI | CTYRAG | 2 cut(s) | 647, 694 |
| SpeI | ACTAGT | 2 cut(s) | 527, 1787 |
| Sse9I | AATT | 8 cut(s) | 213, 642, 957, 1417, 1440, 1582, 1753, 1802 |
| SsiI | CCGC | 2 cut(s) | 98, 1234 |
| SspMI | CTAG | 5 cut(s) | 528, 810, 990, 1779, 1788 |
| SstI | GAGCTC | 1 cut(s) | 17 |
| StyD4I | CCNGG | 5 cut(s) | 340, 558, 1140, 1429, 1738 |
| TaaI | ACNGT | 7 cut(s) | 389, 607, 849, 1299, 1310, 1384, 1515 |
| TaiI | ACGT | 4 cut(s) | 1167, 1280, 1721, 1776 |
| TaqI | TCGA | 8 cut(s) | 498, 513, 594, 610, 826, 1101, 1146, 1797 |
| TasI | AATT | 8 cut(s) | 213, 642, 957, 1417, 1440, 1582, 1753, 1802 |
| TatI | WGTACW | 4 cut(s) | 585, 677, 1413, 1655 |
| TfiI | GAWTC | 3 cut(s) | 110, 1199, 1703 |
| Tru1I | TTAA | 7 cut(s) | 137, 228, 492, 618, 648, 1395, 1407 |
| Tru9I | TTAA | 7 cut(s) | 137, 228, 492, 618, 648, 1395, 1407 |
| TscAI | CASTG | 3 cut(s) | 854, 1304, 1315 |
| TseFI | GTSAC | 2 cut(s) | 76, 442 |
| TseI | GCWGC | 3 cut(s) | 185, 624, 1534 |
| Tsp45I | GTSAC | 2 cut(s) | 76, 442 |
| TspDTI | ATGAA | 5 cut(s) | 183, 500, 909, 998, 1560 |
| TspGWI | ACGGA | 1 cut(s) | 87 |
| TspRI | CASTG | 3 cut(s) | 854, 1304, 1315 |
| Vha464I | CTTAAG | 1 cut(s) | 647 |
| VneI | GTGCAC | 1 cut(s) | 1302 |
| VpaK11BI | GGWCC | 2 cut(s) | 338, 916 |
| VspI | ATTAAT | 1 cut(s) | 1407 |
| XapI | RAATTY | 4 cut(s) | 642, 1440, 1753, 1802 |
| XmiI | GTMKAC | 2 cut(s) | 840, 1146 |
| XspI | CTAG | 5 cut(s) | 528, 810, 990, 1779, 1788 |