Poly(A) polymerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 92 |
| Acc36I | ACCTGC | 1 cut(s) | 763 |
| AccBSI | CCGCTC | 1 cut(s) | 364 |
| AccI | GTMKAC | 1 cut(s) | 276 |
| AccII | CGCG | 1 cut(s) | 66 |
| AciI | CCGC | 1 cut(s) | 364 |
| AclI | AACGTT | 2 cut(s) | 848, 903 |
| AcoI | YGGCCR | 1 cut(s) | 657 |
| AcsI | RAATTY | 3 cut(s) | 570, 883, 932 |
| AcuI | CTGAAG | 1 cut(s) | 254 |
| AfaI | GTAC | 2 cut(s) | 142, 545 |
| AflIII | ACRYGT | 2 cut(s) | 405, 612 |
| AgsI | TTSAA | 2 cut(s) | 17, 833 |
| AhlI | ACTAGT | 1 cut(s) | 917 |
| AjnI | CCWGG | 2 cut(s) | 559, 868 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 18, 50 |
| AluBI | AGCT | 4 cut(s) | 123, 212, 255, 529 |
| AluI | AGCT | 4 cut(s) | 123, 212, 255, 529 |
| Alw21I | GWGCWC | 2 cut(s) | 226, 436 |
| Alw26I | GTCTC | 1 cut(s) | 697 |
| Alw44I | GTGCAC | 1 cut(s) | 432 |
| AoxI | GGCC | 1 cut(s) | 657 |
| ApaLI | GTGCAC | 1 cut(s) | 432 |
| ApeKI | GCWGC | 1 cut(s) | 664 |
| ApoI | RAATTY | 3 cut(s) | 570, 883, 932 |
| AseI | ATTAAT | 1 cut(s) | 537 |
| AspS9I | GGNCC | 1 cut(s) | 46 |
| AsuHPI | GGTGA | 1 cut(s) | 710 |
| AsuII | TTCGAA | 2 cut(s) | 231, 927 |
| AvaII | GGWCC | 1 cut(s) | 46 |
| BaeGI | GKGCMC | 1 cut(s) | 436 |
| BalI | TGGCCA | 1 cut(s) | 659 |
| Bbv12I | GWGCWC | 2 cut(s) | 226, 436 |
| BbvI | GCAGC | 1 cut(s) | 676 |
| BccI | CCATC | 4 cut(s) | 151, 559, 617, 770 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 266, 300 |
| BciT130I | CCWGG | 2 cut(s) | 561, 870 |
| BciVI | GTATCC | 1 cut(s) | 361 |
| BclI | TGATCA | 1 cut(s) | 670 |
| BcoDI | GTCTC | 1 cut(s) | 697 |
| BcuI | ACTAGT | 1 cut(s) | 917 |
| BfaI | CTAG | 3 cut(s) | 120, 909, 918 |
| BfuAI | ACCTGC | 1 cut(s) | 763 |
| BfuI | GTATCC | 1 cut(s) | 361 |
| BisI | GCNGC | 1 cut(s) | 665 |
| BlsI | GCNGC | 1 cut(s) | 666 |
| Bme1390I | CCNGG | 2 cut(s) | 561, 870 |
| Bme18I | GGWCC | 1 cut(s) | 46 |
| BmgT120I | GGNCC | 1 cut(s) | 46 |
| BmiI | GGNNCC | 3 cut(s) | 47, 48, 867 |
| BmrFI | CCNGG | 2 cut(s) | 561, 870 |
| BmsI | GCATC | 3 cut(s) | 767, 797, 865 |
| BpmI | CTGGAG | 4 cut(s) | 777, 876, 884, 891 |
| Bpu14I | TTCGAA | 2 cut(s) | 231, 927 |
| BsaAI | YACGTR | 2 cut(s) | 408, 613 |
| BsaJI | CCNNGG | 1 cut(s) | 560 |
| BsaWI | WCCGGW | 1 cut(s) | 69 |
| Bse1I | ACTGG | 2 cut(s) | 207, 867 |
| BseBI | CCWGG | 2 cut(s) | 561, 870 |
| BseDI | CCNNGG | 1 cut(s) | 560 |
| BseGI | GGATG | 3 cut(s) | 28, 570, 758 |
| BseMII | CTCAG | 1 cut(s) | 180 |
| BseNI | ACTGG | 2 cut(s) | 207, 867 |
| BseSI | GKGCMC | 1 cut(s) | 436 |
| BseXI | GCAGC | 1 cut(s) | 676 |
| BseYI | CCCAGC | 1 cut(s) | 321 |
| Bsh1236I | CGCG | 1 cut(s) | 66 |
| Bsh1285I | CGRYCG | 1 cut(s) | 70 |
| BshFI | GGCC | 1 cut(s) | 659 |
| BsiEI | CGRYCG | 1 cut(s) | 70 |
| BsiHKAI | GWGCWC | 2 cut(s) | 226, 436 |
| BsiSI | CCGG | 2 cut(s) | 70, 743 |
| BslFI | GGGAC | 2 cut(s) | 59, 700 |
| BsmAI | GTCTC | 1 cut(s) | 697 |
| BsmFI | GGGAC | 2 cut(s) | 59, 700 |
| BsnI | GGCC | 1 cut(s) | 659 |
| Bsp119I | TTCGAA | 2 cut(s) | 231, 927 |
| Bsp1286I | GDGCHC | 2 cut(s) | 226, 436 |
| Bsp1407I | TGTACA | 1 cut(s) | 543 |
| Bsp143I | GATC | 3 cut(s) | 301, 670, 724 |
| Bsp68I | TCGCGA | 1 cut(s) | 66 |
| BspACI | CCGC | 1 cut(s) | 364 |
| BspANI | GGCC | 1 cut(s) | 659 |
| BspCNI | CTCAG | 1 cut(s) | 181 |
| BspFNI | CGCG | 1 cut(s) | 66 |
| BspHI | TCATGA | 2 cut(s) | 153, 673 |
| BspLI | GGNNCC | 3 cut(s) | 47, 48, 867 |
| BspMI | ACCTGC | 1 cut(s) | 763 |
| BspT104I | TTCGAA | 2 cut(s) | 231, 927 |
| BsrBI | CCGCTC | 1 cut(s) | 364 |
| BsrGI | TGTACA | 1 cut(s) | 543 |
| BsrI | ACTGG | 2 cut(s) | 207, 867 |
| BssECI | CCNNGG | 1 cut(s) | 560 |
| BssMI | GATC | 3 cut(s) | 301, 670, 724 |
| Bst2UI | CCWGG | 2 cut(s) | 561, 870 |
| Bst4CI | ACNGT | 3 cut(s) | 429, 514, 645 |
| Bst6I | CTCTTC | 2 cut(s) | 12, 713 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 440, 785 |
| BstAUI | TGTACA | 1 cut(s) | 543 |
| BstBAI | YACGTR | 2 cut(s) | 408, 613 |
| BstBI | TTCGAA | 2 cut(s) | 231, 927 |
| BstC8I | GCNNGC | 1 cut(s) | 309 |
| BstDEI | CTNAG | 2 cut(s) | 189, 213 |
| BstF5I | GGATG | 3 cut(s) | 28, 570, 758 |
| BstFNI | CGCG | 1 cut(s) | 66 |
| BstKTI | GATC | 3 cut(s) | 304, 673, 727 |
| BstMAI | GTCTC | 1 cut(s) | 697 |
| BstMBI | GATC | 3 cut(s) | 301, 670, 724 |
| BstMCI | CGRYCG | 1 cut(s) | 70 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 169, 440, 466, 785 |
| BstNI | CCWGG | 2 cut(s) | 561, 870 |
| BstSCI | CCNGG | 2 cut(s) | 559, 868 |
| BstSLI | GKGCMC | 1 cut(s) | 436 |
| BstUI | CGCG | 1 cut(s) | 66 |
| BstV1I | GCAGC | 1 cut(s) | 676 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 655 |
| BsuI | GTATCC | 1 cut(s) | 361 |
| BsuRI | GGCC | 1 cut(s) | 659 |
| BtsCI | GGATG | 3 cut(s) | 28, 570, 758 |
| BtsIMutI | CAGTG | 1 cut(s) | 434 |
| BtuMI | TCGCGA | 1 cut(s) | 66 |
| BveI | ACCTGC | 1 cut(s) | 763 |
| Cac8I | GCNNGC | 1 cut(s) | 309 |
| CciI | TCATGA | 2 cut(s) | 153, 673 |
| Cfr13I | GGNCC | 1 cut(s) | 46 |
| Csp6I | GTAC | 2 cut(s) | 141, 544 |
| CviAII | CATG | 6 cut(s) | 154, 173, 584, 668, 674, 913 |
| CviJI | RGCY | 8 cut(s) | 123, 212, 255, 321, 529, 597, 659, 868 |
| CviKI_1 | RGCY | 8 cut(s) | 123, 212, 255, 321, 529, 597, 659, 868 |
| CviQI | GTAC | 2 cut(s) | 141, 544 |
| DdeI | CTNAG | 2 cut(s) | 189, 213 |
| DpnI | GATC | 3 cut(s) | 303, 672, 726 |
| DpnII | GATC | 3 cut(s) | 301, 670, 724 |
| DraI | TTTAAA | 1 cut(s) | 250 |
| EaeI | YGGCCR | 1 cut(s) | 657 |
| Eam1104I | CTCTTC | 2 cut(s) | 12, 713 |
| EarI | CTCTTC | 2 cut(s) | 12, 713 |
| Eco47I | GGWCC | 1 cut(s) | 46 |
| Eco57I | CTGAAG | 1 cut(s) | 254 |
| EcoO109I | RGGNCCY | 1 cut(s) | 46 |
| EcoRII | CCWGG | 2 cut(s) | 559, 868 |
| FaeI | CATG | 6 cut(s) | 157, 176, 587, 671, 677, 916 |
| FaqI | GGGAC | 2 cut(s) | 59, 700 |
| FatI | CATG | 6 cut(s) | 153, 172, 583, 667, 673, 912 |
| FauI | CCCGC | 1 cut(s) | 357 |
| FauNDI | CATATG | 1 cut(s) | 81 |
| FbaI | TGATCA | 1 cut(s) | 670 |
| FblI | GTMKAC | 1 cut(s) | 276 |
| Fnu4HI | GCNGC | 1 cut(s) | 665 |
| FokI | GGATG | 3 cut(s) | 35, 577, 745 |
| Fsp4HI | GCNGC | 1 cut(s) | 665 |
| FspBI | CTAG | 3 cut(s) | 120, 909, 918 |
| GluI | GCNGC | 1 cut(s) | 665 |
| GsaI | CCCAGC | 1 cut(s) | 325 |
| GsuI | CTGGAG | 4 cut(s) | 777, 876, 884, 891 |
| HaeIII | GGCC | 1 cut(s) | 659 |
| HapII | CCGG | 2 cut(s) | 70, 743 |
| Hin1II | CATG | 6 cut(s) | 157, 176, 587, 671, 677, 916 |
| HincII | GTYRAC | 1 cut(s) | 277 |
| HindII | GTYRAC | 1 cut(s) | 277 |
| HinfI | GANTC | 4 cut(s) | 73, 150, 329, 833 |
| HpaII | CCGG | 2 cut(s) | 70, 743 |
| HphI | GGTGA | 1 cut(s) | 710 |
| Hpy166II | GTNNAC | 3 cut(s) | 277, 434, 632 |
| Hpy188I | TCNGA | 2 cut(s) | 149, 190 |
| Hpy188III | TCNNGA | 7 cut(s) | 65, 154, 620, 648, 674, 794, 855 |
| Hpy8I | GTNNAC | 3 cut(s) | 277, 434, 632 |
| HpyAV | CCTTC | 1 cut(s) | 501 |
| HpyCH4III | ACNGT | 3 cut(s) | 429, 514, 645 |
| HpyCH4IV | ACGT | 4 cut(s) | 407, 612, 848, 903 |
| HpyCH4V | TGCA | 6 cut(s) | 101, 287, 434, 460, 758, 788 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 169, 440, 466, 785 |
| HpyF3I | CTNAG | 2 cut(s) | 189, 213 |
| HpySE526I | ACGT | 4 cut(s) | 407, 612, 848, 903 |
| Hsp92II | CATG | 6 cut(s) | 157, 176, 587, 671, 677, 916 |
| KflI | GGGWCCC | 1 cut(s) | 46 |
| Ksp22I | TGATCA | 1 cut(s) | 670 |
| Kzo9I | GATC | 3 cut(s) | 301, 670, 724 |
| LmnI | GCTCC | 2 cut(s) | 160, 865 |
| Lsp1109I | GCAGC | 1 cut(s) | 676 |
| LweI | GCATC | 3 cut(s) | 767, 797, 865 |
| MaeI | CTAG | 3 cut(s) | 120, 909, 918 |
| MaeII | ACGT | 4 cut(s) | 407, 612, 848, 903 |
| MaeIII | GTNAC | 2 cut(s) | 381, 904 |
| MalI | GATC | 3 cut(s) | 303, 672, 726 |
| MbiI | CCGCTC | 1 cut(s) | 364 |
| MboI | GATC | 3 cut(s) | 301, 670, 724 |
| MboII | GAAGA | 3 cut(s) | 29, 260, 730 |
| MhlI | GDGCHC | 2 cut(s) | 226, 436 |
| MlsI | TGGCCA | 1 cut(s) | 659 |
| MluCI | AATT | 6 cut(s) | 87, 547, 570, 712, 883, 932 |
| MluNI | TGGCCA | 1 cut(s) | 659 |
| MlyI | GAGTC | 2 cut(s) | 67, 159 |
| MnlI | CCTC | 9 cut(s) | 13, 72, 155, 184, 333, 411, 456, 817, 889 |
| Mox20I | TGGCCA | 1 cut(s) | 659 |
| MscI | TGGCCA | 1 cut(s) | 659 |
| MseI | TTAA | 3 cut(s) | 249, 525, 537 |
| MslI | CAYNNNNRTG | 3 cut(s) | 408, 539, 672 |
| Msp20I | TGGCCA | 1 cut(s) | 659 |
| MspI | CCGG | 2 cut(s) | 70, 743 |
| MspR9I | CCNGG | 2 cut(s) | 561, 870 |
| MvaI | CCWGG | 2 cut(s) | 561, 870 |
| MvnI | CGCG | 1 cut(s) | 66 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 169, 440, 466, 785 |
| NdeI | CATATG | 1 cut(s) | 81 |
| NdeII | GATC | 3 cut(s) | 301, 670, 724 |
| NlaIII | CATG | 6 cut(s) | 157, 176, 587, 671, 677, 916 |
| NlaIV | GGNNCC | 3 cut(s) | 47, 48, 867 |
| NruI | TCGCGA | 1 cut(s) | 66 |
| NspV | TTCGAA | 2 cut(s) | 231, 927 |
| PagI | TCATGA | 2 cut(s) | 153, 673 |
| PfeI | GAWTC | 2 cut(s) | 329, 833 |
| PkrI | GCNGC | 1 cut(s) | 666 |
| PleI | GAGTC | 2 cut(s) | 67, 158 |
| PpsI | GAGTC | 2 cut(s) | 67, 158 |
| Ppu21I | YACGTR | 2 cut(s) | 408, 613 |
| PpuMI | RGGWCCY | 1 cut(s) | 46 |
| PshBI | ATTAAT | 1 cut(s) | 537 |
| PsiI | TTATAA | 1 cut(s) | 92 |
| Psp1406I | AACGTT | 2 cut(s) | 848, 903 |
| Psp5II | RGGWCCY | 1 cut(s) | 46 |
| Psp6I | CCWGG | 2 cut(s) | 559, 868 |
| PspFI | CCCAGC | 1 cut(s) | 321 |
| PspGI | CCWGG | 2 cut(s) | 559, 868 |
| PspN4I | GGNNCC | 3 cut(s) | 47, 48, 867 |
| PspPI | GGNCC | 1 cut(s) | 46 |
| PspPPI | RGGWCCY | 1 cut(s) | 46 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 107, 139 |
| RruI | TCGCGA | 1 cut(s) | 66 |
| RsaI | GTAC | 2 cut(s) | 142, 545 |
| RsaNI | GTAC | 2 cut(s) | 141, 544 |
| RseI | CAYNNNNRTG | 3 cut(s) | 408, 539, 672 |
| SalI | GTCGAC | 1 cut(s) | 275 |
| SaqAI | TTAA | 3 cut(s) | 249, 525, 537 |
| SatI | GCNGC | 1 cut(s) | 665 |
| Sau3AI | GATC | 3 cut(s) | 301, 670, 724 |
| Sau96I | GGNCC | 1 cut(s) | 46 |
| SchI | GAGTC | 2 cut(s) | 67, 159 |
| ScrFI | CCNGG | 2 cut(s) | 561, 870 |
| SduI | GDGCHC | 2 cut(s) | 226, 436 |
| SfaNI | GCATC | 3 cut(s) | 767, 797, 865 |
| SfuI | TTCGAA | 2 cut(s) | 231, 927 |
| SinI | GGWCC | 1 cut(s) | 46 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 408, 539, 672 |
| SpeI | ACTAGT | 1 cut(s) | 917 |
| Sse9I | AATT | 6 cut(s) | 87, 547, 570, 712, 883, 932 |
| SsiI | CCGC | 1 cut(s) | 364 |
| SspMI | CTAG | 3 cut(s) | 120, 909, 918 |
| StyD4I | CCNGG | 2 cut(s) | 559, 868 |
| TaaI | ACNGT | 3 cut(s) | 429, 514, 645 |
| TaiI | ACGT | 4 cut(s) | 410, 615, 851, 906 |
| TaqI | TCGA | 3 cut(s) | 231, 276, 927 |
| TasI | AATT | 6 cut(s) | 87, 547, 570, 712, 883, 932 |
| TatI | WGTACW | 1 cut(s) | 543 |
| TfiI | GAWTC | 2 cut(s) | 329, 833 |
| Tru1I | TTAA | 3 cut(s) | 249, 525, 537 |
| Tru9I | TTAA | 3 cut(s) | 249, 525, 537 |
| TscAI | CASTG | 1 cut(s) | 434 |
| TseI | GCWGC | 1 cut(s) | 664 |
| TspDTI | ATGAA | 3 cut(s) | 39, 128, 690 |
| TspRI | CASTG | 1 cut(s) | 434 |
| VneI | GTGCAC | 1 cut(s) | 432 |
| VpaK11BI | GGWCC | 1 cut(s) | 46 |
| VspI | ATTAAT | 1 cut(s) | 537 |
| XapI | RAATTY | 3 cut(s) | 570, 883, 932 |
| XmiI | GTMKAC | 1 cut(s) | 276 |
| XspI | CTAG | 3 cut(s) | 120, 909, 918 |