Rw2G034050

Poly(A) polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000312
Physical Location & Seq
Reverse (-)
55811926 .. 55817637
5712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G034050.1

Sequence Viewer

Length: 2115 bp
ATGGTGAGCTCAGACGGATTGAGTGCTTCGCCGCCGCCGCAGGCGGTGTCGGCAAAGCAATTCGGGGTCACAAAGCCGATTTCGACGGCCGGGCCTACTGAAGCCGATACTCAGAGAACTTTAGAGTTGGAGAAGCTTTTGGTTGGAGCCGGGCTTTACGAAAGCAAAGGGGAAGCTGCCAAGAGAGAGGAGGTTCTTCGCCGAATTGGGCAGATTGTTAAGGATTGGGTAAAGCAACTTACTCAGTTGAGAGGATACACAGATCAAATGGTTGAGGATGCTAATGCTCTTATTTTTACATTTGGTTCCTATAGGCTTGGGGTACATGGTCCTGGGGCTGACATAGACACATTGTGTGTTGGGCCATCTTATGTCAACCGTGAGGAAGACTTCTTCTTTGTATTGCACAACATTCTGGCTGAAATGGAAGAAGTGACTGAACTGCAACCAGTTCCAGATGCCCATGTTCCCGTAATGAGGTTTAAGTTTGATGGAATATCAATCGACCTCCTTTATGCAAGTGTTTCTCTGTTGGTTGTACCAGAAGACCTGGACATTTCTGATGTATCTCTGTTGTACAACATCGATGAGCCTACTGTTCGAAGTCTTAATGGCTGCAGGGTTGCTGATCAAATTCTTAAGCTTGTTCCAAATGTTGAGCATTTCTGTACAACACTCCGATGCTTGAAGTTTTGGGCGAAAAGGCGTGGTGTTTATTCCAATGTTACTGGATTTCTTGGTGGTGTGAACTGGGCTCTCCTCGTTGCCCGAGTGTGTCAACTTTATCCTAATGCAGTTCCTAGCATGCTGGTTTCTCGATTTTTTAGAGTCTACACACAGTGGCGATGGCCAAATCCTGTCATGTTATGTGCTATTGAAGAGGATGAACTTGGTTTTAATGTTTGGGACCCACGCAAAAATCCTCGTGACCGTACTCATCATATGCCAATTATAACTCCTGCATACCCTTGTATGAACTCTAGCTACAATGTTTCTAAAAGTACCCTCCGTGTCATGATGGAGCAGTTTCAGCAGGGAAACAAGATTTCTGAGGAAATAGAGCTGAATAAAGCTAGGTGGTGTGCTCTGTTTGAACCATATTTGTTCTTTGAAAGCTACAAGAACTATCTCCAGGTCGACATCGTTGCAGTTGATGCTGATGATCTGCGTGCTTGGAAAGGCTGGGTGGAATCTCGGTTGAGGCAACTGACTCTAATGATTGAGCGGGATACTTTGGAGATGTTACAATGTCACCCATATCCTCATGAGTATGTTGATACGACCAAACAGTGTGCACATAGTGCTTTCTTTATGGGTTTGCAGAGGAAACAAGGTGAAAAGGTTCAGGGTCAGCAGTTCGATATACGGGGGACTGTTGATGAGTTCAAGCAGTCCATTGAGATGTACAATTTTTGGAAACCTGGGATGGAAATTTATGTTTCTCACGTTCGAAGAAGGCAACTGCCTTCTTATGTGTTTCCTGATGGTTGTAAACGACCGAGACCATCTAGAGCTGTGGCCCTGGAGCATAATAATGAAGTTAGTCCGACGGGTTCTGGTGAGAGACACCTGAAGAGGAAGAATGATCTTGGTGGGGGTTCGCCACCGAAACGACTATCTAATAGCCCTCAGCCCCAGGATTCAATATCTCCTGAAATTATGACTCACAGAGCAGGAAGTGCATCTCCAGAGACTTTTGATAAATGCTTTAGTTTGGAGGTAGTTGATTCAAATAAAAAAACGTTGTCCGGTGAACTGGAGCCTGGAGATGCGACCAATTCCAGTGTTGTTACAAGTGTTGCAAGCATGAGCAGTAGTGAAGATGCTGGATTTGGATCAATGACTGGTAGCTGTGAGGGGAATACTAAAAGTGTTGAAGGAAGCAGTATTGGGAGTAACACTGCTTCTTTATGTGAGGCAGACTCCGAATCTCTTTCAGTTAACGGAGGCGAAAATGGCAACCTTGTGCAAAGTGGATCACAAGAAGGATTAGAGGTTTTGGATTTAAGTTCCTTTACTTTGACTGCTTTTGATTCTTGTTCATTATTTACTTGCCTTCTATTGCAATATCTTTGCTATTCAGATTTCTTTACTCTTAACTCAGAAATTATATGA

Protein Analysis

704

Amino Acids

78.64

Weight (kDa)

5.22

Isoelectric Point (pI)

49.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_NTPase PF20750 22 - 216 2.7e-76 Poly(A) polymerase nucleotidyltransferase domain
NTP_transf_2 PF01909 82 - 171 2e-10 Nucleotidyltransferase domain
PAP_central PF04928 221 - 365 2.6e-69 Poly(A) polymerase central domain
PAP_RNA-bind PF04926 369 - 428 7.7e-11 Poly(A) polymerase predicted RNA binding domain
PAP_RNA-bind PF04926 426 - 501 6.2e-08 Poly(A) polymerase predicted RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000312)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850
fragaria_vesca FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471
malus_domestica MD08G1110400.v1.1 MD09G1202000.v1.1 MD09G1202100.v1.1 MD17G1182800.v1.1
prunus_persica Prupe.1G444600_v2.0.a1 Prupe.3G057600_v2.0.a1 Prupe.3G057600_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1
pyrus_communis pycom08g09190 pycom09g11890 pycom15g08420 pycom17g19200
rosa_chinensis RchiOBHm_Chr2g0140721 RchiOBHm_Chr2g0140741 RchiOBHm_Chr2g0140761 RchiOBHm_Chr6g0306691
rosa_laevigata RLG00000010773 RLG00000019895 RLG00000019896
rosa_multiflora Rmu_co8193342.1_g000001 Rmu_sc0003342.1_g000015 Rmu_sc0003342.1_g000021 Rmu_sc0003342.1_g000026 Rmu_sc0005201.1_g000002 Rmu_sc0017785.1_g000009
rosa_roxburghii Rroxscaffold_2G00104510 Rroxscaffold_2G00104540 Rroxscaffold_7G00161540
rosa_rugosa Rorug02G0364600 Rorug02G0364700 Rorug02G0364800 Rorug02G0364900 Rorug02G0365000 Rorug06G0351600 Rorug06G0351700
rosa_samantha Rh2AG416100 Rh2AG416200 Rh2AG416300 Rh2BG425700 Rh2BG425800 Rh2BG425900 Rh2CG402300 Rh2CG402400 Rh2CG402500 Rh2DG435600 Rh2DG435700 Rh2DG435800 Rh6AG464000 Rh6BG437000 Rh6CG478100 Rh6DG464900
rosa_wichuraiana Rw2G034050 Rw2G034060 Rw6G040420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 953
AccBSI CCGCTC 1 cut(s) 1225
AccI GTMKAC 2 cut(s) 831, 1137
AciI CCGC 5 cut(s) 32, 35, 38, 44, 1225
AclI AACGTT 1 cut(s) 1742
AclWI GGATC 2 cut(s) 1843, 1984
AcoI YGGCCR 2 cut(s) 87, 848
AcsI RAATTY 2 cut(s) 633, 1431
AcuI CTGAAG 2 cut(s) 120, 1592
AdeI CACNNNGTG 3 cut(s) 354, 840, 1301
AfaI GTAC 7 cut(s) 324, 540, 578, 670, 934, 1003, 1406
AfiI CCNNNNNNNGG 1 cut(s) 477
AflII CTTAAG 1 cut(s) 638
AgsI TTSAA 8 cut(s) 688, 878, 1094, 1112, 1387, 1644, 1731, 1877
AjnI CCWGG 7 cut(s) 331, 549, 1131, 1420, 1521, 1635, 1762
Alw21I GWGCWC 3 cut(s) 11, 1087, 1297
Alw26I GTCTC 3 cut(s) 1495, 1558, 1685
Alw44I GTGCAC 1 cut(s) 1293
AlwI GGATC 2 cut(s) 1843, 1984
Ama87I CYCGRG 1 cut(s) 768
AoxI GGCC 5 cut(s) 87, 92, 362, 848, 1518
ApaLI GTGCAC 1 cut(s) 1293
ApeKI GCWGC 2 cut(s) 176, 615
ApoI RAATTY 2 cut(s) 633, 1431
ArsI GACNNNNNNTTYG 2 cut(s) 380, 412
Asp700I GAANNNNTTC 1 cut(s) 1341
AspS9I GGNCC 5 cut(s) 92, 329, 362, 907, 1519
AsuC2I CCSGG 2 cut(s) 91, 151
AsuHPI GGTGA 5 cut(s) 16, 1244, 1346, 1571, 1763
AsuII TTCGAA 2 cut(s) 601, 1450
AvaI CYCGRG 1 cut(s) 768
AvaII GGWCC 2 cut(s) 329, 907
BaeGI GKGCMC 1 cut(s) 1297
BalI TGGCCA 1 cut(s) 850
BanII GRGCYC 2 cut(s) 11, 757
BarI GAAGNNNNNNTAC 2 cut(s) 1888, 1920
BauI CACGAG 1 cut(s) 924
BbsI GAAGAC 2 cut(s) 393, 552
Bbv12I GWGCWC 3 cut(s) 11, 1087, 1297
BbvCI CCTCAGC 1 cut(s) 1629
BbvI GCAGC 2 cut(s) 163, 602
BccI CCATC 7 cut(s) 373, 485, 840, 1012, 1420, 1478, 1513
BceAI ACGGC 1 cut(s) 102
BcgI CGANNNNNNTGC 2 cut(s) 1127, 1161
BciT130I CCWGG 7 cut(s) 333, 551, 1133, 1422, 1523, 1637, 1764
BciVI GTATCC 2 cut(s) 248, 1222
BclI TGATCA 1 cut(s) 628
BcnI CCSGG 2 cut(s) 91, 151
BcoDI GTCTC 3 cut(s) 1495, 1558, 1685
BfaI CTAG 4 cut(s) 801, 981, 1074, 1509
BfmI CTRYAG 2 cut(s) 310, 616
BfrI CTTAAG 1 cut(s) 638
BfuI GTATCC 2 cut(s) 248, 1222
BisI GCNGC 5 cut(s) 32, 35, 38, 177, 616
BlsI GCNGC 5 cut(s) 33, 36, 39, 178, 617
Bme1390I CCNGG 9 cut(s) 91, 151, 333, 551, 1133, 1422, 1523, 1637, 1764
Bme18I GGWCC 2 cut(s) 329, 907
BmeT110I CYCGRG 1 cut(s) 768
BmgT120I GGNCC 5 cut(s) 92, 329, 362, 907, 1519
BmiI GGNNCC 5 cut(s) 148, 307, 908, 909, 1761
BmrFI CCNGG 9 cut(s) 91, 151, 333, 551, 1133, 1422, 1523, 1637, 1764
BmrI ACTGGG 1 cut(s) 760
BmsI GCATC 7 cut(s) 268, 448, 671, 1144, 1691, 1759, 1813
BmuI ACTGGG 1 cut(s) 760
BpiI GAAGAC 2 cut(s) 393, 552
BplI GAGNNNNNCTC 2 cut(s) 1907, 1939
BpmI CTGGAG 5 cut(s) 1115, 1544, 1671, 1778, 1785
Bpu10I CCTNAGC 1 cut(s) 1629
Bpu14I TTCGAA 2 cut(s) 601, 1450
BpuMI CCSGG 2 cut(s) 91, 151
Bsa29I ATCGAT 1 cut(s) 585
BsaBI GATNNNNATC 1 cut(s) 1834
BsaI GGTCTC 1 cut(s) 1495
BsaJI CCNNGG 4 cut(s) 332, 1421, 1521, 1635
BsaWI WCCGGW 1 cut(s) 1748
Bsc4I CCNNNNNNNGG 1 cut(s) 477
Bse1I ACTGG 6 cut(s) 449, 733, 755, 1761, 1782, 1849
Bse8I GATNNNNATC 1 cut(s) 1834
BseBI CCWGG 7 cut(s) 333, 551, 1133, 1422, 1523, 1637, 1764
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 4 cut(s) 332, 1421, 1521, 1635
BseGI GGATG 3 cut(s) 283, 889, 1431
BseJI GATNNNNATC 1 cut(s) 1834
BseLI CCNNNNNNNGG 1 cut(s) 477
BseMII CTCAG 6 cut(s) 24, 125, 257, 1041, 1643, 2115
BseNI ACTGG 6 cut(s) 449, 733, 755, 1761, 1782, 1849
BseRI GAGGAG 2 cut(s) 203, 749
BseSI GKGCMC 1 cut(s) 1297
BseX3I CGGCCG 1 cut(s) 87
BseXI GCAGC 2 cut(s) 163, 602
BseYI CCCAGC 1 cut(s) 1182
Bsh1285I CGRYCG 2 cut(s) 90, 1499
BshFI GGCC 5 cut(s) 89, 94, 364, 850, 1520
BshVI ATCGAT 1 cut(s) 585
BsiEI CGRYCG 2 cut(s) 90, 1499
BsiHKAI GWGCWC 3 cut(s) 11, 1087, 1297
BsiHKCI CYCGRG 1 cut(s) 768
BsiSI CCGG 3 cut(s) 90, 150, 1749
BslFI GGGAC 2 cut(s) 920, 1384
BslI CCNNNNNNNGG 1 cut(s) 477
BsmAI GTCTC 3 cut(s) 1495, 1558, 1685
BsmFI GGGAC 2 cut(s) 920, 1384
BsnI GGCC 5 cut(s) 89, 94, 364, 850, 1520
Bso31I GGTCTC 1 cut(s) 1495
BsoBI CYCGRG 1 cut(s) 768
Bsp119I TTCGAA 2 cut(s) 601, 1450
Bsp1286I GDGCHC 4 cut(s) 11, 757, 1087, 1297
Bsp1407I TGTACA 3 cut(s) 576, 668, 1404
Bsp143I GATC 6 cut(s) 262, 628, 1162, 1585, 1835, 1976
BspACI CCGC 5 cut(s) 32, 35, 38, 44, 1225
BspANI GGCC 5 cut(s) 89, 94, 364, 850, 1520
BspCNI CTCAG 6 cut(s) 23, 124, 256, 1042, 1642, 2114
BspDI ATCGAT 1 cut(s) 585
BspHI TCATGA 2 cut(s) 1014, 1264
BspLI GGNNCC 5 cut(s) 148, 307, 908, 909, 1761
BspMAI CTGCAG 1 cut(s) 620
BspPI GGATC 2 cut(s) 1843, 1984
BspT104I TTCGAA 2 cut(s) 601, 1450
BspTI CTTAAG 1 cut(s) 638
BspTNI GGTCTC 1 cut(s) 1495
BsrBI CCGCTC 1 cut(s) 1225
BsrGI TGTACA 3 cut(s) 576, 668, 1404
BsrI ACTGG 6 cut(s) 449, 733, 755, 1761, 1782, 1849
BssECI CCNNGG 4 cut(s) 332, 1421, 1521, 1635
BssMI GATC 6 cut(s) 262, 628, 1162, 1585, 1835, 1976
BssSI CACGAG 1 cut(s) 924
Bst2BI CACGAG 1 cut(s) 924
Bst2UI CCWGG 7 cut(s) 333, 551, 1133, 1422, 1523, 1637, 1764
Bst4CI ACNGT 6 cut(s) 380, 598, 840, 932, 1290, 1375
Bst6I CTCTTC 2 cut(s) 873, 1568
BstAFI CTTAAG 1 cut(s) 638
BstAPI GCANNNNNTGC 3 cut(s) 1154, 1301, 1679
BstAUI TGTACA 3 cut(s) 576, 668, 1404
BstBI TTCGAA 2 cut(s) 601, 1450
BstC8I GCNNGC 4 cut(s) 42, 806, 1170, 1804
BstDEI CTNAG 6 cut(s) 10, 111, 243, 1050, 1629, 2101
BstF5I GGATG 3 cut(s) 283, 889, 1431
BstKTI GATC 6 cut(s) 265, 631, 1165, 1588, 1838, 1979
BstMAI GTCTC 3 cut(s) 1495, 1558, 1685
BstMBI GATC 6 cut(s) 262, 628, 1162, 1585, 1835, 1976
BstMCI CGRYCG 2 cut(s) 90, 1499
BstMWI GCNNNNNNNGC 7 cut(s) 37, 50, 1030, 1154, 1301, 1679, 1956
BstNI CCWGG 7 cut(s) 333, 551, 1133, 1422, 1523, 1637, 1764
BstNSI RCATGY 1 cut(s) 808
BstSCI CCNGG 9 cut(s) 89, 149, 331, 549, 1131, 1420, 1521, 1635, 1762
BstSFI CTRYAG 2 cut(s) 310, 616
BstSLI GKGCMC 1 cut(s) 1297
BstV1I GCAGC 2 cut(s) 163, 602
BstV2I GAAGAC 2 cut(s) 393, 552
BstZI CGGCCG 1 cut(s) 87
Bsu15I ATCGAT 1 cut(s) 585
BsuI GTATCC 2 cut(s) 248, 1222
BsuRI GGCC 5 cut(s) 89, 94, 364, 850, 1520
BsuTUI ATCGAT 1 cut(s) 585
BtgZI GCGATG 1 cut(s) 859
BtsCI GGATG 3 cut(s) 283, 889, 1431
BtsI GCAGTG 1 cut(s) 1899
BtsIMutI CAGTG 4 cut(s) 845, 1295, 1789, 1899
Cac8I GCNNGC 4 cut(s) 42, 806, 1170, 1804
CciI TCATGA 2 cut(s) 1014, 1264
Cfr13I GGNCC 5 cut(s) 92, 329, 362, 907, 1519
ClaI ATCGAT 1 cut(s) 585
Csp6I GTAC 7 cut(s) 323, 539, 577, 669, 933, 1002, 1405
CviAII CATG 7 cut(s) 326, 464, 805, 862, 1015, 1265, 1807
CviQI GTAC 7 cut(s) 323, 539, 577, 669, 933, 1002, 1405
DdeI CTNAG 6 cut(s) 10, 111, 243, 1050, 1629, 2101
DpnI GATC 6 cut(s) 264, 630, 1164, 1587, 1837, 1978
DpnII GATC 6 cut(s) 262, 628, 1162, 1585, 1835, 1976
DraIII CACNNNGTG 3 cut(s) 354, 840, 1301
EaeI YGGCCR 2 cut(s) 87, 848
EagI CGGCCG 1 cut(s) 87
Eam1104I CTCTTC 2 cut(s) 873, 1568
EarI CTCTTC 2 cut(s) 873, 1568
Ecl136II GAGCTC 1 cut(s) 9
EclXI CGGCCG 1 cut(s) 87
Eco24I GRGCYC 2 cut(s) 11, 757
Eco31I GGTCTC 1 cut(s) 1495
Eco47I GGWCC 2 cut(s) 329, 907
Eco52I CGGCCG 1 cut(s) 87
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 2 cut(s) 120, 1592
Eco88I CYCGRG 1 cut(s) 768
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 907
EcoRII CCWGG 7 cut(s) 331, 549, 1131, 1420, 1521, 1635, 1762
EcoT38I GRGCYC 2 cut(s) 11, 757
FaeI CATG 7 cut(s) 329, 467, 808, 865, 1018, 1268, 1810
FaqI GGGAC 2 cut(s) 920, 1384
FatI CATG 7 cut(s) 325, 463, 804, 861, 1014, 1264, 1806
FauI CCCGC 1 cut(s) 1218
FauNDI CATATG 1 cut(s) 942
FbaI TGATCA 1 cut(s) 628
FblI GTMKAC 2 cut(s) 831, 1137
Fnu4HI GCNGC 5 cut(s) 32, 35, 38, 177, 616
FokI GGATG 3 cut(s) 290, 896, 1438
FriOI GRGCYC 2 cut(s) 11, 757
Fsp4HI GCNGC 5 cut(s) 32, 35, 38, 177, 616
FspBI CTAG 4 cut(s) 801, 981, 1074, 1509
GluI GCNGC 5 cut(s) 32, 35, 38, 177, 616
GsaI CCCAGC 1 cut(s) 1186
GsuI CTGGAG 5 cut(s) 1115, 1544, 1671, 1778, 1785
HaeIII GGCC 5 cut(s) 89, 94, 364, 850, 1520
HapII CCGG 3 cut(s) 90, 150, 1749
Hin1II CATG 7 cut(s) 329, 467, 808, 865, 1018, 1268, 1810
HincII GTYRAC 4 cut(s) 376, 779, 1138, 1942
HindII GTYRAC 4 cut(s) 376, 779, 1138, 1942
HindIII AAGCTT 2 cut(s) 134, 641
HinfI GANTC 9 cut(s) 828, 1190, 1210, 1640, 1663, 1727, 1922, 1928, 2033
HpaI GTTAAC 1 cut(s) 1942
HpaII CCGG 3 cut(s) 90, 150, 1749
HphI GGTGA 5 cut(s) 16, 1244, 1346, 1571, 1763
Hpy166II GTNNAC 9 cut(s) 376, 748, 779, 832, 1138, 1295, 1493, 1754, 1942
Hpy188I TCNGA 9 cut(s) 13, 114, 562, 680, 1051, 1548, 1927, 2083, 2104
Hpy188III TCNNGA 9 cut(s) 455, 816, 926, 1015, 1265, 1481, 1509, 1652, 1688
Hpy8I GTNNAC 9 cut(s) 376, 748, 779, 832, 1138, 1295, 1493, 1754, 1942
Hpy99I CGWCG 2 cut(s) 88, 1552
HpyAV CCTTC 5 cut(s) 1449, 1476, 1871, 1979, 2066
HpyCH4III ACNGT 6 cut(s) 380, 598, 840, 932, 1290, 1375
HpyCH4IV ACGT 2 cut(s) 1446, 1742
HpyF10VI GCNNNNNNNGC 7 cut(s) 37, 50, 1030, 1154, 1301, 1679, 1956
HpyF3I CTNAG 6 cut(s) 10, 111, 243, 1050, 1629, 2101
HpySE526I ACGT 2 cut(s) 1446, 1742
Hsp92II CATG 7 cut(s) 329, 467, 808, 865, 1018, 1268, 1810
KflI GGGWCCC 1 cut(s) 907
Ksp22I TGATCA 1 cut(s) 628
KspAI GTTAAC 1 cut(s) 1942
Kzo9I GATC 6 cut(s) 262, 628, 1162, 1585, 1835, 1976
LmnI GCTCC 4 cut(s) 146, 1021, 1525, 1759
Lsp1109I GCAGC 2 cut(s) 163, 602
LweI GCATC 7 cut(s) 268, 448, 671, 1144, 1691, 1759, 1813
MaeI CTAG 4 cut(s) 801, 981, 1074, 1509
MaeII ACGT 2 cut(s) 1446, 1742
MaeIII GTNAC 8 cut(s) 67, 433, 724, 926, 1242, 1250, 1789, 1895
MalI GATC 6 cut(s) 264, 630, 1164, 1587, 1837, 1978
MbiI CCGCTC 1 cut(s) 1225
MboI GATC 6 cut(s) 262, 628, 1162, 1585, 1835, 1976
MhlI GDGCHC 4 cut(s) 11, 757, 1087, 1297
MlsI TGGCCA 1 cut(s) 850
MluCI AATT 9 cut(s) 59, 204, 633, 948, 1408, 1431, 1656, 1777, 2106
MluNI TGGCCA 1 cut(s) 850
MlyI GAGTC 4 cut(s) 837, 1204, 1657, 1916
MmeI TCCRAC 3 cut(s) 108, 124, 1571
Mox20I TGGCCA 1 cut(s) 850
MroXI GAANNNNTTC 1 cut(s) 1341
MscI TGGCCA 1 cut(s) 850
MseI TTAA 8 cut(s) 219, 483, 609, 639, 897, 1941, 2006, 2097
MslI CAYNNNNRTG 4 cut(s) 679, 1269, 1400, 1533
Msp20I TGGCCA 1 cut(s) 850
MspCI CTTAAG 1 cut(s) 638
MspI CCGG 3 cut(s) 90, 150, 1749
MspR9I CCNGG 9 cut(s) 91, 151, 333, 551, 1133, 1422, 1523, 1637, 1764
MvaI CCWGG 7 cut(s) 333, 551, 1133, 1422, 1523, 1637, 1764
MwoI GCNNNNNNNGC 7 cut(s) 37, 50, 1030, 1154, 1301, 1679, 1956
NciI CCSGG 2 cut(s) 91, 151
NdeI CATATG 1 cut(s) 942
NdeII GATC 6 cut(s) 262, 628, 1162, 1585, 1835, 1976
NlaIII CATG 7 cut(s) 329, 467, 808, 865, 1018, 1268, 1810
NlaIV GGNNCC 5 cut(s) 148, 307, 908, 909, 1761
NmuCI GTSAC 4 cut(s) 67, 433, 926, 1250
NspI RCATGY 1 cut(s) 808
NspV TTCGAA 2 cut(s) 601, 1450
PaeI GCATGC 1 cut(s) 808
PagI TCATGA 2 cut(s) 1014, 1264
PdmI GAANNNNTTC 1 cut(s) 1341
PfeI GAWTC 5 cut(s) 1190, 1640, 1727, 1928, 2033
PkrI GCNGC 5 cut(s) 33, 36, 39, 178, 617
PleI GAGTC 4 cut(s) 836, 1204, 1657, 1916
PpsI GAGTC 4 cut(s) 836, 1204, 1657, 1916
PpuMI RGGWCCY 1 cut(s) 907
PsiI TTATAA 1 cut(s) 953
Psp124BI GAGCTC 1 cut(s) 11
Psp1406I AACGTT 1 cut(s) 1742
Psp5II RGGWCCY 1 cut(s) 907
Psp6I CCWGG 7 cut(s) 331, 549, 1131, 1420, 1521, 1635, 1762
PspFI CCCAGC 1 cut(s) 1182
PspGI CCWGG 7 cut(s) 331, 549, 1131, 1420, 1521, 1635, 1762
PspN4I GGNNCC 5 cut(s) 148, 307, 908, 909, 1761
PspPI GGNCC 5 cut(s) 92, 329, 362, 907, 1519
PspPPI RGGWCCY 1 cut(s) 907
PsrI GAACNNNNNNTAC 2 cut(s) 968, 1000
PstI CTGCAG 1 cut(s) 620
RsaI GTAC 7 cut(s) 324, 540, 578, 670, 934, 1003, 1406
RsaNI GTAC 7 cut(s) 323, 539, 577, 669, 933, 1002, 1405
RseI CAYNNNNRTG 4 cut(s) 679, 1269, 1400, 1533
SacI GAGCTC 1 cut(s) 11
SalI GTCGAC 1 cut(s) 1136
SaqAI TTAA 8 cut(s) 219, 483, 609, 639, 897, 1941, 2006, 2097
SatI GCNGC 5 cut(s) 32, 35, 38, 177, 616
Sau3AI GATC 6 cut(s) 262, 628, 1162, 1585, 1835, 1976
Sau96I GGNCC 5 cut(s) 92, 329, 362, 907, 1519
SchI GAGTC 4 cut(s) 837, 1204, 1657, 1916
ScrFI CCNGG 9 cut(s) 91, 151, 333, 551, 1133, 1422, 1523, 1637, 1764
SduI GDGCHC 4 cut(s) 11, 757, 1087, 1297
SfaNI GCATC 7 cut(s) 268, 448, 671, 1144, 1691, 1759, 1813
SfcI CTRYAG 2 cut(s) 310, 616
SfuI TTCGAA 2 cut(s) 601, 1450
SinI GGWCC 2 cut(s) 329, 907
SmiMI CAYNNNNRTG 4 cut(s) 679, 1269, 1400, 1533
SmlI CTYRAG 1 cut(s) 638
SmoI CTYRAG 1 cut(s) 638
SphI GCATGC 1 cut(s) 808
Sse9I AATT 9 cut(s) 59, 204, 633, 948, 1408, 1431, 1656, 1777, 2106
SsiI CCGC 5 cut(s) 32, 35, 38, 44, 1225
SspMI CTAG 4 cut(s) 801, 981, 1074, 1509
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 9 cut(s) 89, 149, 331, 549, 1131, 1420, 1521, 1635, 1762
TaaI ACNGT 6 cut(s) 380, 598, 840, 932, 1290, 1375
TaiI ACGT 2 cut(s) 1449, 1745
TaqI TCGA 8 cut(s) 83, 504, 585, 601, 817, 1137, 1359, 1450
TaqII GACCGA 1 cut(s) 1513
TasI AATT 9 cut(s) 59, 204, 633, 948, 1408, 1431, 1656, 1777, 2106
TatI WGTACW 3 cut(s) 576, 668, 1404
TauI GCSGC 3 cut(s) 34, 37, 40
TfiI GAWTC 5 cut(s) 1190, 1640, 1727, 1928, 2033
Tru1I TTAA 8 cut(s) 219, 483, 609, 639, 897, 1941, 2006, 2097
Tru9I TTAA 8 cut(s) 219, 483, 609, 639, 897, 1941, 2006, 2097
TscAI CASTG 4 cut(s) 845, 1295, 1789, 1906
TseFI GTSAC 4 cut(s) 67, 433, 926, 1250
TseI GCWGC 2 cut(s) 176, 615
Tsp45I GTSAC 4 cut(s) 67, 433, 926, 1250
TspDTI ATGAA 4 cut(s) 900, 989, 1551, 2031
TspGWI ACGGA 3 cut(s) 30, 998, 1959
TspRI CASTG 4 cut(s) 845, 1295, 1789, 1906
Vha464I CTTAAG 1 cut(s) 638
VneI GTGCAC 1 cut(s) 1293
VpaK11BI GGWCC 2 cut(s) 329, 907
XapI RAATTY 2 cut(s) 633, 1431
XbaI TCTAGA 1 cut(s) 1508
XceI RCATGY 1 cut(s) 808
XmiI GTMKAC 2 cut(s) 831, 1137
XmnI GAANNNNTTC 1 cut(s) 1341
XspI CTAG 4 cut(s) 801, 981, 1074, 1509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.