Rh2AG416100

Poly(A) polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000312
Physical Location & Seq
Reverse (-)
61738719 .. 61743190
4472 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG416100.1

Sequence Viewer

Length: 2097 bp
ATGGTGAGCTCAGACGGATTGAGTGCTTCGCCGCCGCCGCAGGCGGTGTCGGCAAAGCAATTCGGGGTCACAAAGCCGATTTCCACGGCCGGGCCTACTGAAGCCGATACTCAGAGAACTTTAGAGTTGGAGAAGCTTTTGGTTGAAGCCGGGCTTTACGAAAGCAAAGGGGAAGCTGCCAAGAGAGAGGAGGTTCTTCACCGAATTGGGCAGATTGTTAAGGATTGGGTAAAGCAACTTACTCAGTTGAGAGGATACACAGATCAAATGGTTGAGGATGCTAATGCTCTTATTTTTACATTTGGTTCCTATAGGCTTGGGGTACATGGTCCTGGGGCTGACATAGACACATTGTGTGTTGGGCCATCTTATGTCAACCGTGAGGAAGACTTCTTCTTTGTATTGCACAACATTCTGGCTGAAATGGAAGAAGTGACTGAACTGCAACCAGTTCCAGATGCCCATGTTCCCGTAATGAGGTTTAAGTTTGATGGAGTATCAATTGACCTCCTTTATGCAAGTGTTTCTCTGTTGGTTGTACCAGAAGACCTGGACATTTCTGATGTATCTCTGTTGTACAACATTGATGAGCCTACTGTTCGAAGTCTTAATGGCTGCAGGGTTGCTGATCAAATTCTTAAGCTTGTTCCAAATGTTGAGCATTTCTGTACAACACTCCGATGCTTGAAGTTTTGGGCGAAAAGGCGTGGTGTTTATTCCAATGTTACTGGATTTCTTGGTGGTGTGAACTGGGCTCTCCTCGTTGCCCGAGTGTGTCAACTTTATCCTAATGCAGTTCCTAGCATGCTGGTTTCTCGATTTTTTAGAGTCTACACACAGTGGCGATGGCCAAATCCTGTCATGTTATGTGCTATAGAAGAGGATGAACTTGGTTTTAATGTTTGGGACCCACGCAAAAATCCTCGCGACCGTACTCATCATATGCCAATTATAACTCCTGCATACCCTTGTATGAACTCTAGCTACAATGTTTCTAAAAGTACCCTCCGTGTCATGATGGAGCAGTTTCAGCAGGGAAACAAGATTTCTGAGGAAATAGAGCTGAATAAAGCTAGGTGGTGTGCTCTGTTTGAACCATATTTGTTCTTTGATAGCTACAAGAACTATCTCCAGGTCGACATCGTTGCAGTTGATGCTGATGATCTGCGTGCTTGGAAAGGCTGGGTGGAATCTCGGTTGAGGCAACTGACTCTAATGATTGAGCGGGATACTTTGGAGATGTTACAATGTCACCCATATCCTCATGAGTATGTTGATATGACCAAACAGTGTGCACATAGTGCTTTCTTTATGGGTTTGCAGAGGAAACAAGGTGAAAAGGTTCAGGGTCAGCAGTTCGATATACGAGGGACTGTTGATGAGTTCAAGCAGTCCATTGAGATGTACAATTTTTGGAAACCTGGGATGGAAATTTATGTTTCTCACGTTCGAAGAAGGCAACTTCCTTCTTATGTGTTTCCTGATGGTTGTAAACGACCGAGACCATCTAGAGCTGTGGCCCTGCAGCATAATAATGAAGTTAGTCCGACGGGTTCTGGTGAGAGACACCTGAAGAGGAAGAATGATCTTGGTGGGGGTTCGCCACCGAAACGACTATCTATTAGCCCGCAGCCCCAGGATTCAATATCTCCTGAAATTATGACTCACAGAGCAGGAAGTGCATCTCCAGAGACTTTTGATAAATGCTTTAGTTTGGAGGTAGTTGATTCAAATAAAAAAATGTTGTCCGGTGAACTGGAGCCTGGAGATGCGACCAATTCCAGTGTTGTTACAAGTGTTACAAGCATGAGCAGTAGTGAAGATGCTGGATTTGGATCAATGACTGGTAGCTGTGAGGGGAATACTAAAAGTGTTGAAGGAAGCAGTATTGGGAGTAACACTGCTTCTTTATGTGAGGCAGACTCTGAATCTCTTTCAGTTAACGGAGGCGAAAATGGCAACCTTGTGCAAAGTGGATCACAAGAAGGATTAGAGTTGAATGCTGCATTTGGGATGATACTTAATCCTAGTGAGGGAGTAAGATCGGAAGGTGTGCAGAAAGCAGTGATGAGGTTGGGTTTAACCTCAATGGCCTGA

Protein Analysis

698

Amino Acids

77.75

Weight (kDa)

5.44

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_NTPase PF20750 22 - 216 2.1e-76 Poly(A) polymerase nucleotidyltransferase domain
NTP_transf_2 PF01909 82 - 171 2e-10 Nucleotidyltransferase domain
PAP_central PF04928 221 - 365 2.5e-69 Poly(A) polymerase central domain
PAP_RNA-bind PF04926 369 - 426 1.6e-10 Poly(A) polymerase predicted RNA binding domain
PAP_RNA-bind PF04926 428 - 501 1e-07 Poly(A) polymerase predicted RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000312)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850
fragaria_vesca FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471
malus_domestica MD08G1110400.v1.1 MD09G1202000.v1.1 MD09G1202100.v1.1 MD17G1182800.v1.1
prunus_persica Prupe.1G444600_v2.0.a1 Prupe.3G057600_v2.0.a1 Prupe.3G057600_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1
pyrus_communis pycom08g09190 pycom09g11890 pycom15g08420 pycom17g19200
rosa_chinensis RchiOBHm_Chr2g0140721 RchiOBHm_Chr2g0140741 RchiOBHm_Chr2g0140761 RchiOBHm_Chr6g0306691
rosa_laevigata RLG00000010773 RLG00000019895 RLG00000019896
rosa_multiflora Rmu_co8193342.1_g000001 Rmu_sc0003342.1_g000015 Rmu_sc0003342.1_g000021 Rmu_sc0003342.1_g000026 Rmu_sc0005201.1_g000002 Rmu_sc0017785.1_g000009
rosa_roxburghii Rroxscaffold_2G00104510 Rroxscaffold_2G00104540 Rroxscaffold_7G00161540
rosa_rugosa Rorug02G0364600 Rorug02G0364700 Rorug02G0364800 Rorug02G0364900 Rorug02G0365000 Rorug06G0351600 Rorug06G0351700
rosa_samantha Rh2AG416100 Rh2AG416200 Rh2AG416300 Rh2BG425700 Rh2BG425800 Rh2BG425900 Rh2CG402300 Rh2CG402400 Rh2CG402500 Rh2DG435600 Rh2DG435700 Rh2DG435800 Rh6AG464000 Rh6BG437000 Rh6CG478100 Rh6DG464900
rosa_wichuraiana Rw2G034050 Rw2G034060 Rw6G040420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 953
AccBSI CCGCTC 1 cut(s) 1225
AccI GTMKAC 2 cut(s) 831, 1137
AccII CGCG 1 cut(s) 927
AciI CCGC 6 cut(s) 32, 35, 38, 44, 1225, 1628
AclWI GGATC 2 cut(s) 1843, 1984
AcoI YGGCCR 2 cut(s) 87, 848
AcsI RAATTY 2 cut(s) 633, 1431
AcuI CTGAAG 2 cut(s) 120, 1592
AdeI CACNNNGTG 3 cut(s) 354, 840, 1301
AfaI GTAC 7 cut(s) 324, 540, 578, 670, 934, 1003, 1406
AfiI CCNNNNNNNGG 3 cut(s) 90, 477, 2033
AflII CTTAAG 1 cut(s) 638
AgsI TTSAA 8 cut(s) 146, 688, 1094, 1387, 1644, 1731, 1877, 1999
AjnI CCWGG 6 cut(s) 331, 549, 1131, 1420, 1635, 1762
Alw21I GWGCWC 3 cut(s) 11, 1087, 1297
Alw26I GTCTC 3 cut(s) 1495, 1558, 1685
Alw44I GTGCAC 1 cut(s) 1293
AlwI GGATC 2 cut(s) 1843, 1984
AlwNI CAGNNNCTG 1 cut(s) 1925
Ama87I CYCGRG 1 cut(s) 768
AoxI GGCC 6 cut(s) 87, 92, 362, 848, 1518, 2091
ApaLI GTGCAC 1 cut(s) 1293
ApeKI GCWGC 5 cut(s) 176, 615, 1525, 1630, 2003
ApoI RAATTY 2 cut(s) 633, 1431
ArsI GACNNNNNNTTYG 2 cut(s) 380, 412
Asp700I GAANNNNTTC 1 cut(s) 1341
AspS9I GGNCC 5 cut(s) 92, 329, 362, 907, 1519
AsuC2I CCSGG 2 cut(s) 91, 151
AsuHPI GGTGA 6 cut(s) 16, 191, 1244, 1346, 1571, 1763
AsuII TTCGAA 2 cut(s) 601, 1450
AvaI CYCGRG 1 cut(s) 768
AvaII GGWCC 2 cut(s) 329, 907
BaeGI GKGCMC 1 cut(s) 1297
BalI TGGCCA 1 cut(s) 850
BanII GRGCYC 2 cut(s) 11, 757
BarI GAAGNNNNNNTAC 2 cut(s) 1888, 1920
BbsI GAAGAC 2 cut(s) 393, 552
Bbv12I GWGCWC 3 cut(s) 11, 1087, 1297
BbvI GCAGC 5 cut(s) 163, 602, 1537, 1642, 1990
BccI CCATC 7 cut(s) 373, 485, 840, 1012, 1420, 1478, 1513
BceAI ACGGC 1 cut(s) 102
BcgI CGANNNNNNTGC 2 cut(s) 1127, 1161
BciT130I CCWGG 6 cut(s) 333, 551, 1133, 1422, 1637, 1764
BciVI GTATCC 2 cut(s) 248, 1222
BclI TGATCA 1 cut(s) 628
BcnI CCSGG 2 cut(s) 91, 151
BcoDI GTCTC 3 cut(s) 1495, 1558, 1685
BfaI CTAG 5 cut(s) 801, 981, 1074, 1509, 2028
BfmI CTRYAG 4 cut(s) 310, 616, 873, 1523
BfrI CTTAAG 1 cut(s) 638
BfuI GTATCC 2 cut(s) 248, 1222
BisI GCNGC 8 cut(s) 32, 35, 38, 177, 616, 1526, 1631, 2004
BlsI GCNGC 8 cut(s) 33, 36, 39, 178, 617, 1527, 1632, 2005
Bme1390I CCNGG 8 cut(s) 91, 151, 333, 551, 1133, 1422, 1637, 1764
Bme18I GGWCC 2 cut(s) 329, 907
BmeT110I CYCGRG 1 cut(s) 768
BmgT120I GGNCC 5 cut(s) 92, 329, 362, 907, 1519
BmiI GGNNCC 4 cut(s) 307, 908, 909, 1761
BmrFI CCNGG 8 cut(s) 91, 151, 333, 551, 1133, 1422, 1637, 1764
BmrI ACTGGG 1 cut(s) 760
BmsI GCATC 7 cut(s) 268, 448, 671, 1144, 1691, 1759, 1813
BmuI ACTGGG 1 cut(s) 760
BpiI GAAGAC 2 cut(s) 393, 552
BplI GAGNNNNNCTC 2 cut(s) 1907, 1939
BpmI CTGGAG 4 cut(s) 1115, 1671, 1778, 1785
Bpu14I TTCGAA 2 cut(s) 601, 1450
BpuMI CCSGG 2 cut(s) 91, 151
BsaBI GATNNNNATC 1 cut(s) 1834
BsaI GGTCTC 1 cut(s) 1495
BsaJI CCNNGG 4 cut(s) 84, 332, 1421, 1635
BsaWI WCCGGW 1 cut(s) 1748
Bsc4I CCNNNNNNNGG 3 cut(s) 90, 477, 2033
Bse1I ACTGG 6 cut(s) 449, 733, 755, 1761, 1782, 1849
Bse8I GATNNNNATC 1 cut(s) 1834
BseBI CCWGG 6 cut(s) 333, 551, 1133, 1422, 1637, 1764
BseDI CCNNGG 4 cut(s) 84, 332, 1421, 1635
BseGI GGATG 4 cut(s) 283, 889, 1431, 2019
BseJI GATNNNNATC 1 cut(s) 1834
BseLI CCNNNNNNNGG 3 cut(s) 90, 477, 2033
BseMII CTCAG 4 cut(s) 24, 125, 257, 1041
BseNI ACTGG 6 cut(s) 449, 733, 755, 1761, 1782, 1849
BseRI GAGGAG 2 cut(s) 203, 749
BseSI GKGCMC 1 cut(s) 1297
BseX3I CGGCCG 1 cut(s) 87
BseXI GCAGC 5 cut(s) 163, 602, 1537, 1642, 1990
BseYI CCCAGC 1 cut(s) 1182
BsgI GTGCAG 1 cut(s) 2075
Bsh1236I CGCG 1 cut(s) 927
Bsh1285I CGRYCG 3 cut(s) 90, 931, 1499
BshFI GGCC 6 cut(s) 89, 94, 364, 850, 1520, 2093
BsiEI CGRYCG 3 cut(s) 90, 931, 1499
BsiHKAI GWGCWC 3 cut(s) 11, 1087, 1297
BsiHKCI CYCGRG 1 cut(s) 768
BsiSI CCGG 3 cut(s) 90, 150, 1749
BslFI GGGAC 2 cut(s) 920, 1384
BslI CCNNNNNNNGG 3 cut(s) 90, 477, 2033
BsmAI GTCTC 3 cut(s) 1495, 1558, 1685
BsmFI GGGAC 2 cut(s) 920, 1384
BsmI GAATGC 1 cut(s) 2005
BsnI GGCC 6 cut(s) 89, 94, 364, 850, 1520, 2093
Bso31I GGTCTC 1 cut(s) 1495
BsoBI CYCGRG 1 cut(s) 768
Bsp119I TTCGAA 2 cut(s) 601, 1450
Bsp1286I GDGCHC 4 cut(s) 11, 757, 1087, 1297
Bsp1407I TGTACA 3 cut(s) 576, 668, 1404
Bsp143I GATC 7 cut(s) 262, 628, 1162, 1585, 1835, 1976, 2042
Bsp68I TCGCGA 1 cut(s) 927
BspACI CCGC 6 cut(s) 32, 35, 38, 44, 1225, 1628
BspANI GGCC 6 cut(s) 89, 94, 364, 850, 1520, 2093
BspCNI CTCAG 4 cut(s) 23, 124, 256, 1042
BspFNI CGCG 1 cut(s) 927
BspHI TCATGA 2 cut(s) 1014, 1264
BspLI GGNNCC 4 cut(s) 307, 908, 909, 1761
BspMAI CTGCAG 2 cut(s) 620, 1527
BspPI GGATC 2 cut(s) 1843, 1984
BspT104I TTCGAA 2 cut(s) 601, 1450
BspTI CTTAAG 1 cut(s) 638
BspTNI GGTCTC 1 cut(s) 1495
BsrBI CCGCTC 1 cut(s) 1225
BsrGI TGTACA 3 cut(s) 576, 668, 1404
BsrI ACTGG 6 cut(s) 449, 733, 755, 1761, 1782, 1849
BssECI CCNNGG 4 cut(s) 84, 332, 1421, 1635
BssMI GATC 7 cut(s) 262, 628, 1162, 1585, 1835, 1976, 2042
Bst2UI CCWGG 6 cut(s) 333, 551, 1133, 1422, 1637, 1764
Bst4CI ACNGT 6 cut(s) 380, 598, 840, 932, 1290, 1375
Bst6I CTCTTC 2 cut(s) 873, 1568
BstAFI CTTAAG 1 cut(s) 638
BstAPI GCANNNNNTGC 3 cut(s) 1154, 1301, 1679
BstAUI TGTACA 3 cut(s) 576, 668, 1404
BstBI TTCGAA 2 cut(s) 601, 1450
BstC8I GCNNGC 4 cut(s) 42, 806, 1170, 1628
BstDEI CTNAG 4 cut(s) 10, 111, 243, 1050
BstDSI CCRYGG 1 cut(s) 84
BstF5I GGATG 4 cut(s) 283, 889, 1431, 2019
BstFNI CGCG 1 cut(s) 927
BstKTI GATC 7 cut(s) 265, 631, 1165, 1588, 1838, 1979, 2045
BstMAI GTCTC 3 cut(s) 1495, 1558, 1685
BstMBI GATC 7 cut(s) 262, 628, 1162, 1585, 1835, 1976, 2042
BstMCI CGRYCG 3 cut(s) 90, 931, 1499
BstMWI GCNNNNNNNGC 7 cut(s) 37, 50, 1030, 1154, 1301, 1679, 1956
BstNI CCWGG 6 cut(s) 333, 551, 1133, 1422, 1637, 1764
BstNSI RCATGY 1 cut(s) 808
BstSCI CCNGG 8 cut(s) 89, 149, 331, 549, 1131, 1420, 1635, 1762
BstSFI CTRYAG 4 cut(s) 310, 616, 873, 1523
BstSLI GKGCMC 1 cut(s) 1297
BstUI CGCG 1 cut(s) 927
BstV1I GCAGC 5 cut(s) 163, 602, 1537, 1642, 1990
BstV2I GAAGAC 2 cut(s) 393, 552
BstZI CGGCCG 1 cut(s) 87
BsuI GTATCC 2 cut(s) 248, 1222
BsuRI GGCC 6 cut(s) 89, 94, 364, 850, 1520, 2093
BtgI CCRYGG 1 cut(s) 84
BtgZI GCGATG 1 cut(s) 859
BtsCI GGATG 4 cut(s) 283, 889, 1431, 2019
BtsI GCAGTG 2 cut(s) 1899, 2070
BtsIMutI CAGTG 5 cut(s) 845, 1295, 1789, 1899, 2070
BtuMI TCGCGA 1 cut(s) 927
Cac8I GCNNGC 4 cut(s) 42, 806, 1170, 1628
CaiI CAGNNNCTG 1 cut(s) 1925
CciI TCATGA 2 cut(s) 1014, 1264
Cfr13I GGNCC 5 cut(s) 92, 329, 362, 907, 1519
Csp6I GTAC 7 cut(s) 323, 539, 577, 669, 933, 1002, 1405
CviAII CATG 7 cut(s) 326, 464, 805, 862, 1015, 1265, 1807
CviQI GTAC 7 cut(s) 323, 539, 577, 669, 933, 1002, 1405
DdeI CTNAG 4 cut(s) 10, 111, 243, 1050
DpnI GATC 7 cut(s) 264, 630, 1164, 1587, 1837, 1978, 2044
DpnII GATC 7 cut(s) 262, 628, 1162, 1585, 1835, 1976, 2042
DraIII CACNNNGTG 3 cut(s) 354, 840, 1301
EaeI YGGCCR 2 cut(s) 87, 848
EagI CGGCCG 1 cut(s) 87
Eam1104I CTCTTC 2 cut(s) 873, 1568
EarI CTCTTC 2 cut(s) 873, 1568
Ecl136II GAGCTC 1 cut(s) 9
EclXI CGGCCG 1 cut(s) 87
Eco24I GRGCYC 2 cut(s) 11, 757
Eco31I GGTCTC 1 cut(s) 1495
Eco47I GGWCC 2 cut(s) 329, 907
Eco52I CGGCCG 1 cut(s) 87
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 2 cut(s) 120, 1592
Eco88I CYCGRG 1 cut(s) 768
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 907
EcoRII CCWGG 6 cut(s) 331, 549, 1131, 1420, 1635, 1762
EcoT38I GRGCYC 2 cut(s) 11, 757
FaeI CATG 7 cut(s) 329, 467, 808, 865, 1018, 1268, 1810
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FaqI GGGAC 2 cut(s) 920, 1384
FatI CATG 7 cut(s) 325, 463, 804, 861, 1014, 1264, 1806
FauI CCCGC 2 cut(s) 1218, 1635
FauNDI CATATG 1 cut(s) 942
FbaI TGATCA 1 cut(s) 628
FblI GTMKAC 2 cut(s) 831, 1137
Fnu4HI GCNGC 8 cut(s) 32, 35, 38, 177, 616, 1526, 1631, 2004
FokI GGATG 4 cut(s) 290, 896, 1438, 2026
FriOI GRGCYC 2 cut(s) 11, 757
Fsp4HI GCNGC 8 cut(s) 32, 35, 38, 177, 616, 1526, 1631, 2004
FspBI CTAG 5 cut(s) 801, 981, 1074, 1509, 2028
GluI GCNGC 8 cut(s) 32, 35, 38, 177, 616, 1526, 1631, 2004
GsaI CCCAGC 1 cut(s) 1186
GsuI CTGGAG 4 cut(s) 1115, 1671, 1778, 1785
HaeIII GGCC 6 cut(s) 89, 94, 364, 850, 1520, 2093
HapII CCGG 3 cut(s) 90, 150, 1749
Hin1II CATG 7 cut(s) 329, 467, 808, 865, 1018, 1268, 1810
HincII GTYRAC 4 cut(s) 376, 779, 1138, 1942
HindII GTYRAC 4 cut(s) 376, 779, 1138, 1942
HindIII AAGCTT 2 cut(s) 134, 641
HinfI GANTC 8 cut(s) 828, 1190, 1210, 1640, 1663, 1727, 1922, 1928
HpaI GTTAAC 1 cut(s) 1942
HpaII CCGG 3 cut(s) 90, 150, 1749
HphI GGTGA 6 cut(s) 16, 191, 1244, 1346, 1571, 1763
Hpy166II GTNNAC 9 cut(s) 376, 748, 779, 832, 1138, 1295, 1493, 1754, 1942
Hpy188I TCNGA 8 cut(s) 13, 114, 562, 680, 1051, 1548, 1927, 2047
Hpy188III TCNNGA 9 cut(s) 455, 816, 926, 1015, 1265, 1481, 1509, 1652, 1688
Hpy8I GTNNAC 9 cut(s) 376, 748, 779, 832, 1138, 1295, 1493, 1754, 1942
Hpy99I CGWCG 1 cut(s) 1552
HpyAV CCTTC 5 cut(s) 1449, 1476, 1871, 1979, 2042
HpyCH4III ACNGT 6 cut(s) 380, 598, 840, 932, 1290, 1375
HpyCH4IV ACGT 1 cut(s) 1446
HpyF10VI GCNNNNNNNGC 7 cut(s) 37, 50, 1030, 1154, 1301, 1679, 1956
HpyF3I CTNAG 4 cut(s) 10, 111, 243, 1050
HpySE526I ACGT 1 cut(s) 1446
Hsp92II CATG 7 cut(s) 329, 467, 808, 865, 1018, 1268, 1810
KflI GGGWCCC 1 cut(s) 907
Ksp22I TGATCA 1 cut(s) 628
KspAI GTTAAC 1 cut(s) 1942
Kzo9I GATC 7 cut(s) 262, 628, 1162, 1585, 1835, 1976, 2042
LmnI GCTCC 2 cut(s) 1021, 1759
Lsp1109I GCAGC 5 cut(s) 163, 602, 1537, 1642, 1990
LweI GCATC 7 cut(s) 268, 448, 671, 1144, 1691, 1759, 1813
MaeI CTAG 5 cut(s) 801, 981, 1074, 1509, 2028
MaeII ACGT 1 cut(s) 1446
MaeIII GTNAC 8 cut(s) 67, 433, 724, 1242, 1250, 1789, 1798, 1895
MalI GATC 7 cut(s) 264, 630, 1164, 1587, 1837, 1978, 2044
MbiI CCGCTC 1 cut(s) 1225
MboI GATC 7 cut(s) 262, 628, 1162, 1585, 1835, 1976, 2042
MfeI CAATTG 1 cut(s) 501
MhlI GDGCHC 4 cut(s) 11, 757, 1087, 1297
MlsI TGGCCA 1 cut(s) 850
MluCI AATT 9 cut(s) 59, 204, 501, 633, 948, 1408, 1431, 1656, 1777
MluNI TGGCCA 1 cut(s) 850
MlyI GAGTC 4 cut(s) 837, 1204, 1657, 1916
MmeI TCCRAC 2 cut(s) 108, 1571
Mox20I TGGCCA 1 cut(s) 850
MroXI GAANNNNTTC 1 cut(s) 1341
MscI TGGCCA 1 cut(s) 850
MseI TTAA 8 cut(s) 219, 483, 609, 639, 897, 1941, 2022, 2081
MslI CAYNNNNRTG 4 cut(s) 679, 1269, 1400, 1533
Msp20I TGGCCA 1 cut(s) 850
MspCI CTTAAG 1 cut(s) 638
MspI CCGG 3 cut(s) 90, 150, 1749
MspR9I CCNGG 8 cut(s) 91, 151, 333, 551, 1133, 1422, 1637, 1764
MunI CAATTG 1 cut(s) 501
Mva1269I GAATGC 1 cut(s) 2005
MvaI CCWGG 6 cut(s) 333, 551, 1133, 1422, 1637, 1764
MvnI CGCG 1 cut(s) 927
MwoI GCNNNNNNNGC 7 cut(s) 37, 50, 1030, 1154, 1301, 1679, 1956
NciI CCSGG 2 cut(s) 91, 151
NdeI CATATG 1 cut(s) 942
NdeII GATC 7 cut(s) 262, 628, 1162, 1585, 1835, 1976, 2042
NlaIII CATG 7 cut(s) 329, 467, 808, 865, 1018, 1268, 1810
NlaIV GGNNCC 4 cut(s) 307, 908, 909, 1761
NmuCI GTSAC 3 cut(s) 67, 433, 1250
NruI TCGCGA 1 cut(s) 927
NspI RCATGY 1 cut(s) 808
NspV TTCGAA 2 cut(s) 601, 1450
PaeI GCATGC 1 cut(s) 808
PagI TCATGA 2 cut(s) 1014, 1264
PctI GAATGC 1 cut(s) 2005
PdmI GAANNNNTTC 1 cut(s) 1341
PfeI GAWTC 4 cut(s) 1190, 1640, 1727, 1928
PkrI GCNGC 8 cut(s) 33, 36, 39, 178, 617, 1527, 1632, 2005
PleI GAGTC 4 cut(s) 836, 1204, 1657, 1916
PpsI GAGTC 4 cut(s) 836, 1204, 1657, 1916
PpuMI RGGWCCY 1 cut(s) 907
PsiI TTATAA 1 cut(s) 953
Psp124BI GAGCTC 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 907
Psp6I CCWGG 6 cut(s) 331, 549, 1131, 1420, 1635, 1762
PspFI CCCAGC 1 cut(s) 1182
PspGI CCWGG 6 cut(s) 331, 549, 1131, 1420, 1635, 1762
PspN4I GGNNCC 4 cut(s) 307, 908, 909, 1761
PspPI GGNCC 5 cut(s) 92, 329, 362, 907, 1519
PspPPI RGGWCCY 1 cut(s) 907
PsrI GAACNNNNNNTAC 2 cut(s) 968, 1000
PstI CTGCAG 2 cut(s) 620, 1527
PstNI CAGNNNCTG 1 cut(s) 1925
RruI TCGCGA 1 cut(s) 927
RsaI GTAC 7 cut(s) 324, 540, 578, 670, 934, 1003, 1406
RsaNI GTAC 7 cut(s) 323, 539, 577, 669, 933, 1002, 1405
RseI CAYNNNNRTG 4 cut(s) 679, 1269, 1400, 1533
SacI GAGCTC 1 cut(s) 11
SalI GTCGAC 1 cut(s) 1136
SaqAI TTAA 8 cut(s) 219, 483, 609, 639, 897, 1941, 2022, 2081
SatI GCNGC 8 cut(s) 32, 35, 38, 177, 616, 1526, 1631, 2004
Sau3AI GATC 7 cut(s) 262, 628, 1162, 1585, 1835, 1976, 2042
Sau96I GGNCC 5 cut(s) 92, 329, 362, 907, 1519
SchI GAGTC 4 cut(s) 837, 1204, 1657, 1916
ScrFI CCNGG 8 cut(s) 91, 151, 333, 551, 1133, 1422, 1637, 1764
SduI GDGCHC 4 cut(s) 11, 757, 1087, 1297
SfaNI GCATC 7 cut(s) 268, 448, 671, 1144, 1691, 1759, 1813
SfcI CTRYAG 4 cut(s) 310, 616, 873, 1523
SfuI TTCGAA 2 cut(s) 601, 1450
SinI GGWCC 2 cut(s) 329, 907
SmiMI CAYNNNNRTG 4 cut(s) 679, 1269, 1400, 1533
SmlI CTYRAG 1 cut(s) 638
SmoI CTYRAG 1 cut(s) 638
SphI GCATGC 1 cut(s) 808
Sse9I AATT 9 cut(s) 59, 204, 501, 633, 948, 1408, 1431, 1656, 1777
SsiI CCGC 6 cut(s) 32, 35, 38, 44, 1225, 1628
SspMI CTAG 5 cut(s) 801, 981, 1074, 1509, 2028
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 8 cut(s) 89, 149, 331, 549, 1131, 1420, 1635, 1762
TaaI ACNGT 6 cut(s) 380, 598, 840, 932, 1290, 1375
TaiI ACGT 1 cut(s) 1449
TaqI TCGA 5 cut(s) 601, 817, 1137, 1359, 1450
TaqII GACCGA 1 cut(s) 1513
TasI AATT 9 cut(s) 59, 204, 501, 633, 948, 1408, 1431, 1656, 1777
TatI WGTACW 3 cut(s) 576, 668, 1404
TauI GCSGC 3 cut(s) 34, 37, 40
TfiI GAWTC 4 cut(s) 1190, 1640, 1727, 1928
Tru1I TTAA 8 cut(s) 219, 483, 609, 639, 897, 1941, 2022, 2081
Tru9I TTAA 8 cut(s) 219, 483, 609, 639, 897, 1941, 2022, 2081
TscAI CASTG 5 cut(s) 845, 1295, 1789, 1906, 2070
TseFI GTSAC 3 cut(s) 67, 433, 1250
TseI GCWGC 5 cut(s) 176, 615, 1525, 1630, 2003
Tsp45I GTSAC 3 cut(s) 67, 433, 1250
TspDTI ATGAA 3 cut(s) 900, 989, 1551
TspGWI ACGGA 3 cut(s) 30, 998, 1959
TspRI CASTG 5 cut(s) 845, 1295, 1789, 1906, 2070
Vha464I CTTAAG 1 cut(s) 638
VneI GTGCAC 1 cut(s) 1293
VpaK11BI GGWCC 2 cut(s) 329, 907
XapI RAATTY 2 cut(s) 633, 1431
XbaI TCTAGA 1 cut(s) 1508
XceI RCATGY 1 cut(s) 808
XmiI GTMKAC 2 cut(s) 831, 1137
XmnI GAANNNNTTC 1 cut(s) 1341
XspI CTAG 5 cut(s) 801, 981, 1074, 1509, 2028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.