RLG00000019896

Poly(A) polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000312
Physical Location & Seq
Reverse (-)
58240953 .. 58246116
5164 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019896

Sequence Viewer

Length: 2100 bp
ATGAGCTCAGAGGGGTTGAGTGCTTCGCCACCGCCTACGCAGGCCGTGGTCGGTAAGCAATACGGAGTGACAAAGCCACTTTTGACAGCGGGGCCAACTGAATCCGATATTCAGAGAACTTTAGAGTTAAAGAAGATTGTTAAGGATTGGGTGAAGCAACTTACTCGGTTGAGAGGATACACAGATCAAATGGTTGAGGATGCTAATCCTCTTATTTTTACATTTGGTTTGTATAGGCTTGGGGTACATGGTCCTGGGGATGGGATAGACACATTGTGTGTTGGGCCATCAGAAGACTTCTTTATTGTATTGCACGACACTCTGGCAGAAATGGAAGAAGTGACTGAACTGCAACCAGTTCCAGATGCTTATGTTCCCATAATGAAGTTTAAGTTCGATGGAATATCAATCGACCTCCTTTATGCAAGTGTTTCTCTGTTGGACCTGGCCATTTCTGATGTATCTCTGTTGTACAACATCGATGAGCCTACTGTTCAAAGTCTTAATGGCTGCAGGGATGCTGATCAAATTCTTAAGCTTGTTCCAAATGTTGAGGATTTCTGTACAACACTCCGATGCTTGAGGTTTTGGGCGAAAAGGCGTGGTGTTTATTCCAATGTTGCTGGATTTCTTGGTGGTGTGAACTGGGCTCTCCTCGTTGCCCGAGTGTGTCAACTTTATCCTAATGCAGTTCCTAGCGTGCTGGTTTCTCGATTTTTTAGAGTCTACACACAGTGGCGATGGCCAAATCCTGTCATGTTATGTGCTATTGAAGAGGATGAACTTGGTTTTGGTGTTTGGGACCCCCGCAAAAATCCCCGCGATCAGACTCATCTTATGCCAATTATAACTCCTGCATACCCTTGTATGAACTCTAGCTACAATGTTTCTAAAAGTACCCTTCGTGTCATGATGGAGCAATTTCAGCATGGAAACAAGATTTGTGAGGAAATAGAACTGGATGAAGCTAGGTGGTGTGCTCTCTTCGAACCATGTTTGTTCTTTGAAAGCTACAAGAACTATCTCCAGGTCGACATCGTTGCAGTTGATGCTGATGATCTGCGTGCATGGAAAGGATGTGTGGAATCTCGGTTGAGGCAACTGACTCTAATGATTGAGCGGGATAATTTGGAGATGTTACAATGGCACCCATATCCTCATGAATATGTTGATACGACCAAACAGTGTGCACATAGTGCTTTTTTTATGGGTTTTCAGAGGAAAAAGGGTGAAAAGCTTAATGGTCATCAATTCAATATACAAGGGACTGTTGATGAGTTCAAGCAGTCCATTGTTGATATGTACATTTTTTGGAAACCTGGGATGGAATTCTATGTTTCTCACGTTCGTAGAAGGCACCTTCCTTCTTACGTGTTTCCTGATGGTTATAAACGACCTAGACGCTCTAGAGTTTTGGCCCAGCAACATGATCATGAAGTTGGTGCAACGAGTTCCGGTGAGAGACACCTGAAGAGGAAGAATGATCTTGGTGGGGGTTCGCCGGAGAAACGACTATCCATTATCCCTCACAAAGCAGGAAGTGCATCTCCAAAAACTTTGGATAAATGTGTTAGTATGGAGGTAGTTGAATCAAACTCTGGAGAACTGGAGCCTGGAGATGCCTCCAATTCCAGTGTTATTACAAATGTTACTAGCATCACTGGAGAGCACCGGAACCCTAGTAGGCTCGTCAGAGCCGTTGCTATCCAGACACAACCGGCCTTTGCCATTCCAGCGCTTCCGGCGGTCAGCCTCACCCTTCAGCCATTGGAAGAATACGACGGAGCCTGGTGTGGGTGGTCACCAAACTCAAGTCCGCCTCCAAGCCTCGACCCGACTTCACTCCCAAATCCTCTGCACCAATCCGATCACCACCGCCGGACCCACGTTTGCTGCGATTGCACGATCACCTATCCAGAACCCGACGTCGACACCAAACAGACCACTGCCCGTCAAAGCTCTGCCACTGATCCGCGACCGTGGCTTCTCCACCACAATTCGACTGGAGAAGTCCTCCCCGCCTACTTTACAAACCGAGATAGCGACGCCGGAGAGTCAAAGGCAAACGGAGGAAGCCGCCGCTCTGCTTCACGAAACTAG

Protein Analysis

700

Amino Acids

78.62

Weight (kDa)

6.05

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_NTPase PF20750 42 - 181 1.7e-44 Poly(A) polymerase nucleotidyltransferase domain
PAP_central PF04928 187 - 330 5.1e-68 Poly(A) polymerase central domain
PAP_RNA-bind PF04926 334 - 392 2.8e-10 Poly(A) polymerase predicted RNA binding domain
PAP_RNA-bind PF04926 391 - 467 3.6e-06 Poly(A) polymerase predicted RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000312)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850
fragaria_vesca FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471
malus_domestica MD08G1110400.v1.1 MD09G1202000.v1.1 MD09G1202100.v1.1 MD17G1182800.v1.1
prunus_persica Prupe.1G444600_v2.0.a1 Prupe.3G057600_v2.0.a1 Prupe.3G057600_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1
pyrus_communis pycom08g09190 pycom09g11890 pycom15g08420 pycom17g19200
rosa_chinensis RchiOBHm_Chr2g0140721 RchiOBHm_Chr2g0140741 RchiOBHm_Chr2g0140761 RchiOBHm_Chr6g0306691
rosa_laevigata RLG00000010773 RLG00000019895 RLG00000019896
rosa_multiflora Rmu_co8193342.1_g000001 Rmu_sc0003342.1_g000015 Rmu_sc0003342.1_g000021 Rmu_sc0003342.1_g000026 Rmu_sc0005201.1_g000002 Rmu_sc0017785.1_g000009
rosa_roxburghii Rroxscaffold_2G00104510 Rroxscaffold_2G00104540 Rroxscaffold_7G00161540
rosa_rugosa Rorug02G0364600 Rorug02G0364700 Rorug02G0364800 Rorug02G0364900 Rorug02G0365000 Rorug06G0351600 Rorug06G0351700
rosa_samantha Rh2AG416100 Rh2AG416200 Rh2AG416300 Rh2BG425700 Rh2BG425800 Rh2BG425900 Rh2CG402300 Rh2CG402400 Rh2CG402500 Rh2DG435600 Rh2DG435700 Rh2DG435800 Rh6AG464000 Rh6BG437000 Rh6CG478100 Rh6DG464900
rosa_wichuraiana Rw2G034050 Rw2G034060 Rw6G040420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 848, 1389
AatII GACGTC 1 cut(s) 1929
AccB1I GGYRCC 2 cut(s) 1146, 1356
AccBSI CCGCTC 2 cut(s) 1120, 2082
AccI GTMKAC 3 cut(s) 726, 1032, 1929
AccII CGCG 2 cut(s) 822, 1975
AclWI GGATC 1 cut(s) 1964
AcoI YGGCCR 2 cut(s) 447, 743
AcsI RAATTY 2 cut(s) 528, 1328
AcuI CTGAAG 2 cut(s) 1490, 1745
AcyI GRCGYC 2 cut(s) 1926, 2046
AdeI CACNNNGTG 3 cut(s) 276, 735, 1196
AfaI GTAC 5 cut(s) 246, 473, 565, 898, 1304
AfeI AGCGCT 1 cut(s) 1737
AfiI CCNNNNNNNGG 2 cut(s) 260, 1794
AflII CTTAAG 1 cut(s) 533
AflIII ACRYGT 1 cut(s) 1371
AgsI TTSAA 6 cut(s) 497, 773, 1007, 1255, 1282, 1589
AjnI CCWGG 6 cut(s) 253, 444, 1026, 1318, 1612, 1787
AjuI GAANNNNNNNTTGG 2 cut(s) 774, 806
AluBI AGCT 7 cut(s) 6, 538, 879, 968, 1011, 1237, 1959
AluI AGCT 7 cut(s) 6, 538, 879, 968, 1011, 1237, 1959
Alw21I GWGCWC 4 cut(s) 8, 982, 1192, 1671
Alw26I GTCTC 1 cut(s) 1456
Alw44I GTGCAC 1 cut(s) 1188
AlwI GGATC 1 cut(s) 1964
Ama87I CYCGRG 1 cut(s) 663
Aor51HI AGCGCT 1 cut(s) 1737
AoxI GGCC 7 cut(s) 42, 92, 284, 447, 743, 1416, 1719
ApaLI GTGCAC 1 cut(s) 1188
ApeKI GCWGC 2 cut(s) 510, 1893
ApoI RAATTY 2 cut(s) 528, 1328
AspLEI GCGC 1 cut(s) 1738
AspS9I GGNCC 7 cut(s) 92, 251, 284, 442, 802, 1417, 1881
AsuHPI GGTGA 7 cut(s) 163, 1241, 1469, 1747, 1794, 1862, 1900
AsuII TTCGAA 1 cut(s) 987
AvaI CYCGRG 1 cut(s) 663
AvaII GGWCC 4 cut(s) 251, 442, 802, 1881
BaeGI GKGCMC 1 cut(s) 1192
BalI TGGCCA 2 cut(s) 449, 745
BanI GGYRCC 2 cut(s) 1146, 1356
BanII GRGCYC 2 cut(s) 8, 652
BbsI GAAGAC 1 cut(s) 300
Bbv12I GWGCWC 4 cut(s) 8, 982, 1192, 1671
BbvI GCAGC 2 cut(s) 497, 1880
BccI CCATC 7 cut(s) 254, 295, 392, 735, 907, 1318, 1376
BceAI ACGGC 2 cut(s) 29, 1682
BcgI CGANNNNNNTGC 4 cut(s) 146, 180, 1022, 1056
BciT130I CCWGG 6 cut(s) 255, 446, 1028, 1320, 1614, 1789
BciVI GTATCC 1 cut(s) 170
BclI TGATCA 2 cut(s) 523, 1429
BcoDI GTCTC 1 cut(s) 1456
BfaI CTAG 8 cut(s) 696, 876, 969, 1398, 1407, 1653, 1680, 2098
BfmI CTRYAG 1 cut(s) 511
BfoI RGCGCY 1 cut(s) 1739
BfrI CTTAAG 1 cut(s) 533
BfuI GTATCC 1 cut(s) 170
BisI GCNGC 4 cut(s) 511, 1894, 2077, 2080
BlsI GCNGC 4 cut(s) 512, 1895, 2078, 2081
Bme1390I CCNGG 6 cut(s) 255, 446, 1028, 1320, 1614, 1789
Bme18I GGWCC 4 cut(s) 251, 442, 802, 1881
BmeT110I CYCGRG 1 cut(s) 663
BmgT120I GGNCC 7 cut(s) 92, 251, 284, 442, 802, 1417, 1881
BmiI GGNNCC 9 cut(s) 93, 803, 804, 1148, 1358, 1611, 1676, 1786, 1883
BmrFI CCNGG 6 cut(s) 255, 446, 1028, 1320, 1614, 1789
BmrI ACTGGG 1 cut(s) 655
BmsI GCATC 8 cut(s) 190, 355, 508, 566, 1039, 1553, 1609, 1665
BmuI ACTGGG 1 cut(s) 655
BpiI GAAGAC 1 cut(s) 300
BplI GAGNNNNNCTC 2 cut(s) 1998, 2030
BpmI CTGGAG 6 cut(s) 1010, 1620, 1628, 1635, 1683, 2025
Bpu14I TTCGAA 1 cut(s) 987
BpuEI CTTGAG 2 cut(s) 601, 1795
Bsa29I ATCGAT 1 cut(s) 480
BsaAI YACGTR 1 cut(s) 1372
BsaBI GATNNNNATC 2 cut(s) 204, 522
BsaHI GRCGYC 2 cut(s) 1926, 2046
BsaJI CCNNGG 4 cut(s) 45, 254, 1319, 1979
BsaWI WCCGGW 2 cut(s) 1454, 1671
Bsc4I CCNNNNNNNGG 2 cut(s) 260, 1794
Bse118I RCCGGY 1 cut(s) 1717
Bse1I ACTGG 7 cut(s) 356, 650, 963, 1611, 1632, 1666, 2008
Bse8I GATNNNNATC 2 cut(s) 204, 522
BseBI CCWGG 6 cut(s) 255, 446, 1028, 1320, 1614, 1789
BseCI ATCGAT 1 cut(s) 480
BseDI CCNNGG 4 cut(s) 45, 254, 1319, 1979
BseGI GGATG 7 cut(s) 205, 265, 523, 784, 967, 1082, 1329
BseJI GATNNNNATC 2 cut(s) 204, 522
BseLI CCNNNNNNNGG 2 cut(s) 260, 1794
BseMII CTCAG 1 cut(s) 21
BseNI ACTGG 7 cut(s) 356, 650, 963, 1611, 1632, 1666, 2008
BseRI GAGGAG 1 cut(s) 644
BseSI GKGCMC 1 cut(s) 1192
BseXI GCAGC 2 cut(s) 497, 1880
BseYI CCCAGC 1 cut(s) 1419
BsgI GTGCAG 1 cut(s) 1841
Bsh1236I CGCG 2 cut(s) 822, 1975
Bsh1285I CGRYCG 1 cut(s) 1979
BshFI GGCC 7 cut(s) 44, 94, 286, 449, 745, 1418, 1721
BshNI GGYRCC 2 cut(s) 1146, 1356
BshVI ATCGAT 1 cut(s) 480
BsiEI CGRYCG 1 cut(s) 1979
BsiHKAI GWGCWC 4 cut(s) 8, 982, 1192, 1671
BsiHKCI CYCGRG 1 cut(s) 663
BsiSI CCGG 7 cut(s) 1455, 1502, 1672, 1718, 1742, 1879, 2049
BslFI GGGAC 2 cut(s) 815, 1279
BslI CCNNNNNNNGG 2 cut(s) 260, 1794
BsmAI GTCTC 1 cut(s) 1456
BsmFI GGGAC 2 cut(s) 815, 1279
BsnI GGCC 7 cut(s) 44, 94, 286, 449, 745, 1418, 1721
BsoBI CYCGRG 1 cut(s) 663
Bsp119I TTCGAA 1 cut(s) 987
Bsp1286I GDGCHC 5 cut(s) 8, 652, 982, 1192, 1671
Bsp1407I TGTACA 3 cut(s) 471, 563, 1302
Bsp143I GATC 9 cut(s) 184, 523, 823, 1057, 1429, 1483, 1867, 1905, 1969
BspANI GGCC 7 cut(s) 44, 94, 286, 449, 745, 1418, 1721
BspCNI CTCAG 1 cut(s) 20
BspDI ATCGAT 1 cut(s) 480
BspFNI CGCG 2 cut(s) 822, 1975
BspHI TCATGA 3 cut(s) 909, 1159, 1432
BspLI GGNNCC 9 cut(s) 93, 803, 804, 1148, 1358, 1611, 1676, 1786, 1883
BspMAI CTGCAG 1 cut(s) 515
BspPI GGATC 1 cut(s) 1964
BspT104I TTCGAA 1 cut(s) 987
BspT107I GGYRCC 2 cut(s) 1146, 1356
BspTI CTTAAG 1 cut(s) 533
BsrBI CCGCTC 2 cut(s) 1120, 2082
BsrFI RCCGGY 1 cut(s) 1717
BsrGI TGTACA 3 cut(s) 471, 563, 1302
BsrI ACTGG 7 cut(s) 356, 650, 963, 1611, 1632, 1666, 2008
BssAI RCCGGY 1 cut(s) 1717
BssECI CCNNGG 4 cut(s) 45, 254, 1319, 1979
BssMI GATC 9 cut(s) 184, 523, 823, 1057, 1429, 1483, 1867, 1905, 1969
BssNI GRCGYC 2 cut(s) 1926, 2046
Bst2UI CCWGG 6 cut(s) 255, 446, 1028, 1320, 1614, 1789
Bst4CI ACNGT 5 cut(s) 493, 735, 1185, 1270, 1980
Bst6I CTCTTC 3 cut(s) 768, 989, 1466
BstACI GRCGYC 2 cut(s) 1926, 2046
BstAFI CTTAAG 1 cut(s) 533
BstAPI GCANNNNNTGC 3 cut(s) 1049, 1196, 1541
BstAUI TGTACA 3 cut(s) 471, 563, 1302
BstBAI YACGTR 1 cut(s) 1372
BstBI TTCGAA 1 cut(s) 987
BstC8I GCNNGC 3 cut(s) 42, 701, 1065
BstDEI CTNAG 1 cut(s) 7
BstDSI CCRYGG 2 cut(s) 45, 1979
BstEII GGTNACC 1 cut(s) 1800
BstF5I GGATG 7 cut(s) 205, 265, 523, 784, 967, 1082, 1329
BstFNI CGCG 2 cut(s) 822, 1975
BstH2I RGCGCY 1 cut(s) 1739
BstHHI GCGC 1 cut(s) 1738
BstKTI GATC 9 cut(s) 187, 526, 826, 1060, 1432, 1486, 1870, 1908, 1972
BstMAI GTCTC 1 cut(s) 1456
BstMBI GATC 9 cut(s) 184, 523, 823, 1057, 1429, 1483, 1867, 1905, 1969
BstMCI CGRYCG 1 cut(s) 1979
BstMWI GCNNNNNNNGC 8 cut(s) 925, 1049, 1196, 1541, 1733, 1742, 1899, 1981
BstNI CCWGG 6 cut(s) 255, 446, 1028, 1320, 1614, 1789
BstPI GGTNACC 1 cut(s) 1800
BstSCI CCNGG 6 cut(s) 253, 444, 1026, 1318, 1612, 1787
BstSFI CTRYAG 1 cut(s) 511
BstSLI GKGCMC 1 cut(s) 1192
BstUI CGCG 2 cut(s) 822, 1975
BstV1I GCAGC 2 cut(s) 497, 1880
BstV2I GAAGAC 1 cut(s) 300
Bsu15I ATCGAT 1 cut(s) 480
BsuI GTATCC 1 cut(s) 170
BsuRI GGCC 7 cut(s) 44, 94, 286, 449, 745, 1418, 1721
BsuTUI ATCGAT 1 cut(s) 480
BtgI CCRYGG 2 cut(s) 45, 1979
BtgZI GCGATG 1 cut(s) 754
BtsCI GGATG 7 cut(s) 205, 265, 523, 784, 967, 1082, 1329
BtsI GCAGTG 1 cut(s) 1944
BtsIMutI CAGTG 6 cut(s) 740, 1190, 1639, 1659, 1944, 1965
Cac8I GCNNGC 3 cut(s) 42, 701, 1065
CciI TCATGA 3 cut(s) 909, 1159, 1432
CfoI GCGC 1 cut(s) 1738
Cfr10I RCCGGY 1 cut(s) 1717
Cfr13I GGNCC 7 cut(s) 92, 251, 284, 442, 802, 1417, 1881
ClaI ATCGAT 1 cut(s) 480
CseI GACGC 2 cut(s) 1410, 2054
Csp6I GTAC 5 cut(s) 245, 472, 564, 897, 1303
CviAII CATG 9 cut(s) 248, 757, 910, 929, 993, 1068, 1160, 1427, 1433
CviQI GTAC 5 cut(s) 245, 472, 564, 897, 1303
DdeI CTNAG 1 cut(s) 7
DpnI GATC 9 cut(s) 186, 525, 825, 1059, 1431, 1485, 1869, 1907, 1971
DpnII GATC 9 cut(s) 184, 523, 823, 1057, 1429, 1483, 1867, 1905, 1969
DraIII CACNNNGTG 3 cut(s) 276, 735, 1196
EaeI YGGCCR 2 cut(s) 447, 743
Eam1104I CTCTTC 3 cut(s) 768, 989, 1466
EarI CTCTTC 3 cut(s) 768, 989, 1466
EciI GGCGGA 1 cut(s) 1806
Ecl136II GAGCTC 1 cut(s) 6
Eco24I GRGCYC 2 cut(s) 8, 652
Eco47I GGWCC 4 cut(s) 251, 442, 802, 1881
Eco47III AGCGCT 1 cut(s) 1737
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 2 cut(s) 1490, 1745
Eco88I CYCGRG 1 cut(s) 663
Eco91I GGTNACC 1 cut(s) 1800
EcoICRI GAGCTC 1 cut(s) 6
EcoO109I RGGNCCY 1 cut(s) 802
EcoO65I GGTNACC 1 cut(s) 1800
EcoRI GAATTC 1 cut(s) 1328
EcoRII CCWGG 6 cut(s) 253, 444, 1026, 1318, 1612, 1787
EcoT38I GRGCYC 2 cut(s) 8, 652
FaeI CATG 9 cut(s) 251, 760, 913, 932, 996, 1071, 1163, 1430, 1436
FaqI GGGAC 2 cut(s) 815, 1279
FatI CATG 9 cut(s) 247, 756, 909, 928, 992, 1067, 1159, 1426, 1432
FauI CCCGC 5 cut(s) 82, 815, 827, 1113, 2026
FbaI TGATCA 2 cut(s) 523, 1429
FblI GTMKAC 3 cut(s) 726, 1032, 1929
Fnu4HI GCNGC 4 cut(s) 511, 1894, 2077, 2080
FokI GGATG 7 cut(s) 212, 272, 530, 791, 974, 1089, 1336
FriOI GRGCYC 2 cut(s) 8, 652
Fsp4HI GCNGC 4 cut(s) 511, 1894, 2077, 2080
FspBI CTAG 8 cut(s) 696, 876, 969, 1398, 1407, 1653, 1680, 2098
GlaI GCGC 1 cut(s) 1737
GluI GCNGC 4 cut(s) 511, 1894, 2077, 2080
GsaI CCCAGC 1 cut(s) 1423
GsuI CTGGAG 6 cut(s) 1010, 1620, 1628, 1635, 1683, 2025
HaeII RGCGCY 1 cut(s) 1739
HaeIII GGCC 7 cut(s) 44, 94, 286, 449, 745, 1418, 1721
HapII CCGG 7 cut(s) 1455, 1502, 1672, 1718, 1742, 1879, 2049
HgaI GACGC 2 cut(s) 1410, 2054
HhaI GCGC 1 cut(s) 1738
Hin1I GRCGYC 2 cut(s) 1926, 2046
Hin1II CATG 9 cut(s) 251, 760, 913, 932, 996, 1071, 1163, 1430, 1436
Hin6I GCGC 1 cut(s) 1736
HinP1I GCGC 1 cut(s) 1736
HincII GTYRAC 3 cut(s) 674, 1033, 1930
HindII GTYRAC 3 cut(s) 674, 1033, 1930
HindIII AAGCTT 2 cut(s) 536, 1235
HinfI GANTC 7 cut(s) 101, 723, 829, 1085, 1105, 1589, 2054
HpaII CCGG 7 cut(s) 1455, 1502, 1672, 1718, 1742, 1879, 2049
HphI GGTGA 7 cut(s) 163, 1241, 1469, 1747, 1794, 1862, 1900
Hpy166II GTNNAC 6 cut(s) 643, 674, 727, 1033, 1190, 1930
Hpy8I GTNNAC 6 cut(s) 643, 674, 727, 1033, 1190, 1930
Hpy99I CGWCG 4 cut(s) 1784, 1928, 1931, 2048
HpyAV CCTTC 5 cut(s) 911, 1347, 1370, 1374, 1769
HpyCH4III ACNGT 5 cut(s) 493, 735, 1185, 1270, 1980
HpyCH4IV ACGT 4 cut(s) 1344, 1371, 1887, 1926
HpyF10VI GCNNNNNNNGC 8 cut(s) 925, 1049, 1196, 1541, 1733, 1742, 1899, 1981
HpyF3I CTNAG 1 cut(s) 7
HpySE526I ACGT 4 cut(s) 1344, 1371, 1887, 1926
Hsp92I GRCGYC 2 cut(s) 1926, 2046
Hsp92II CATG 9 cut(s) 251, 760, 913, 932, 996, 1071, 1163, 1430, 1436
HspAI GCGC 1 cut(s) 1736
KflI GGGWCCC 1 cut(s) 802
Ksp22I TGATCA 2 cut(s) 523, 1429
Kzo9I GATC 9 cut(s) 184, 523, 823, 1057, 1429, 1483, 1867, 1905, 1969
LmnI GCTCC 3 cut(s) 916, 1609, 1784
Lsp1109I GCAGC 2 cut(s) 497, 1880
LweI GCATC 8 cut(s) 190, 355, 508, 566, 1039, 1553, 1609, 1665
MaeI CTAG 8 cut(s) 696, 876, 969, 1398, 1407, 1653, 1680, 2098
MaeII ACGT 4 cut(s) 1344, 1371, 1887, 1926
MaeIII GTNAC 5 cut(s) 67, 340, 1137, 1648, 1800
MalI GATC 9 cut(s) 186, 525, 825, 1059, 1431, 1485, 1869, 1907, 1971
MbiI CCGCTC 2 cut(s) 1120, 2082
MboI GATC 9 cut(s) 184, 523, 823, 1057, 1429, 1483, 1867, 1905, 1969
MboII GAAGA 8 cut(s) 145, 305, 347, 785, 976, 1483, 1489, 1784
MhlI GDGCHC 5 cut(s) 8, 652, 982, 1192, 1671
MlsI TGGCCA 2 cut(s) 449, 745
MluCI AATT 8 cut(s) 528, 843, 920, 1126, 1250, 1328, 1627, 1996
MluNI TGGCCA 2 cut(s) 449, 745
MlyI GAGTC 4 cut(s) 732, 823, 1099, 2063
MmeI TCCRAC 1 cut(s) 420
Mox20I TGGCCA 2 cut(s) 449, 745
MscI TGGCCA 2 cut(s) 449, 745
MseI TTAA 6 cut(s) 128, 141, 390, 504, 534, 1239
MslI CAYNNNNRTG 3 cut(s) 574, 1164, 1431
Msp20I TGGCCA 2 cut(s) 449, 745
MspA1I CMGCKG 1 cut(s) 89
MspCI CTTAAG 1 cut(s) 533
MspI CCGG 7 cut(s) 1455, 1502, 1672, 1718, 1742, 1879, 2049
MspR9I CCNGG 6 cut(s) 255, 446, 1028, 1320, 1614, 1789
MvaI CCWGG 6 cut(s) 255, 446, 1028, 1320, 1614, 1789
MvnI CGCG 2 cut(s) 822, 1975
MwoI GCNNNNNNNGC 8 cut(s) 925, 1049, 1196, 1541, 1733, 1742, 1899, 1981
NdeII GATC 9 cut(s) 184, 523, 823, 1057, 1429, 1483, 1867, 1905, 1969
NlaIII CATG 9 cut(s) 251, 760, 913, 932, 996, 1071, 1163, 1430, 1436
NlaIV GGNNCC 9 cut(s) 93, 803, 804, 1148, 1358, 1611, 1676, 1786, 1883
NmuCI GTSAC 3 cut(s) 67, 340, 1800
NspV TTCGAA 1 cut(s) 987
PagI TCATGA 3 cut(s) 909, 1159, 1432
PcsI WCGNNNNNNNCGW 2 cut(s) 1695, 1893
PfeI GAWTC 3 cut(s) 101, 1085, 1589
PkrI GCNGC 4 cut(s) 512, 1895, 2078, 2081
PleI GAGTC 4 cut(s) 731, 823, 1099, 2062
PpsI GAGTC 4 cut(s) 731, 823, 1099, 2062
Ppu21I YACGTR 1 cut(s) 1372
PpuMI RGGWCCY 1 cut(s) 802
PsiI TTATAA 2 cut(s) 848, 1389
Psp124BI GAGCTC 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 802
Psp6I CCWGG 6 cut(s) 253, 444, 1026, 1318, 1612, 1787
PspEI GGTNACC 1 cut(s) 1800
PspFI CCCAGC 1 cut(s) 1419
PspGI CCWGG 6 cut(s) 253, 444, 1026, 1318, 1612, 1787
PspN4I GGNNCC 9 cut(s) 93, 803, 804, 1148, 1358, 1611, 1676, 1786, 1883
PspPI GGNCC 7 cut(s) 92, 251, 284, 442, 802, 1417, 1881
PspPPI RGGWCCY 1 cut(s) 802
PsrI GAACNNNNNNTAC 2 cut(s) 863, 895
PstI CTGCAG 1 cut(s) 515
RsaI GTAC 5 cut(s) 246, 473, 565, 898, 1304
RsaNI GTAC 5 cut(s) 245, 472, 564, 897, 1303
RseI CAYNNNNRTG 3 cut(s) 574, 1164, 1431
SacI GAGCTC 1 cut(s) 8
SalI GTCGAC 2 cut(s) 1031, 1928
SaqAI TTAA 6 cut(s) 128, 141, 390, 504, 534, 1239
SatI GCNGC 4 cut(s) 511, 1894, 2077, 2080
Sau3AI GATC 9 cut(s) 184, 523, 823, 1057, 1429, 1483, 1867, 1905, 1969
Sau96I GGNCC 7 cut(s) 92, 251, 284, 442, 802, 1417, 1881
SchI GAGTC 4 cut(s) 732, 823, 1099, 2063
ScrFI CCNGG 6 cut(s) 255, 446, 1028, 1320, 1614, 1789
SduI GDGCHC 5 cut(s) 8, 652, 982, 1192, 1671
SfaNI GCATC 8 cut(s) 190, 355, 508, 566, 1039, 1553, 1609, 1665
SfcI CTRYAG 1 cut(s) 511
SfuI TTCGAA 1 cut(s) 987
SinI GGWCC 4 cut(s) 251, 442, 802, 1881
SmiMI CAYNNNNRTG 3 cut(s) 574, 1164, 1431
SmlI CTYRAG 3 cut(s) 533, 580, 1810
SmoI CTYRAG 3 cut(s) 533, 580, 1810
Sse9I AATT 8 cut(s) 528, 843, 920, 1126, 1250, 1328, 1627, 1996
SspMI CTAG 8 cut(s) 696, 876, 969, 1398, 1407, 1653, 1680, 2098
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 6 cut(s) 253, 444, 1026, 1318, 1612, 1787
TaaI ACNGT 5 cut(s) 493, 735, 1185, 1270, 1980
TaiI ACGT 4 cut(s) 1347, 1374, 1890, 1929
TaqI TCGA 9 cut(s) 396, 411, 480, 712, 987, 1032, 1830, 1929, 2000
TasI AATT 8 cut(s) 528, 843, 920, 1126, 1250, 1328, 1627, 1996
TatI WGTACW 3 cut(s) 471, 563, 1302
TauI GCSGC 2 cut(s) 2079, 2082
TfiI GAWTC 3 cut(s) 101, 1085, 1589
Tru1I TTAA 6 cut(s) 128, 141, 390, 504, 534, 1239
Tru9I TTAA 6 cut(s) 128, 141, 390, 504, 534, 1239
TscAI CASTG 6 cut(s) 740, 1190, 1639, 1666, 1951, 1972
TseFI GTSAC 3 cut(s) 67, 340, 1800
TseI GCWGC 2 cut(s) 510, 1893
Tsp45I GTSAC 3 cut(s) 67, 340, 1800
TspDTI ATGAA 6 cut(s) 398, 795, 884, 978, 1176, 1449
TspGWI ACGGA 3 cut(s) 78, 1797, 2082
TspRI CASTG 6 cut(s) 740, 1190, 1639, 1666, 1951, 1972
Vha464I CTTAAG 1 cut(s) 533
VneI GTGCAC 1 cut(s) 1188
VpaK11BI GGWCC 4 cut(s) 251, 442, 802, 1881
XapI RAATTY 2 cut(s) 528, 1328
XbaI TCTAGA 1 cut(s) 1406
XcmI CCANNNNNNNNNTGG 1 cut(s) 2000
XmiI GTMKAC 3 cut(s) 726, 1032, 1929
XspI CTAG 8 cut(s) 696, 876, 969, 1398, 1407, 1653, 1680, 2098
ZraI GACGTC 1 cut(s) 1927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.