Poly(A) polymerase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| PAP_NTPase | PF20750 | 25 - 219 | 5.9e-73 | Poly(A) polymerase nucleotidyltransferase domain |
| NTP_transf_2 | PF01909 | 92 - 174 | 3e-09 | Nucleotidyltransferase domain |
| PAP_central | PF04928 | 224 - 368 | 7.8e-69 | Poly(A) polymerase central domain |
| PAP_RNA-bind | PF04926 | 372 - 430 | 2.4e-13 | Poly(A) polymerase predicted RNA binding domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1132 |
| Acc16I | TGCGCA | 1 cut(s) | 1305 |
| Acc36I | ACCTGC | 2 cut(s) | 1132, 2244 |
| Acc65I | GGTACC | 2 cut(s) | 1799, 1807 |
| AccB1I | GGYRCC | 2 cut(s) | 1799, 1807 |
| AccI | GTMKAC | 2 cut(s) | 1280, 1753 |
| AccII | CGCG | 1 cut(s) | 717 |
| AciI | CCGC | 5 cut(s) | 5, 59, 717, 1694, 1837 |
| AclWI | GGATC | 5 cut(s) | 322, 564, 911, 924, 945 |
| AcoI | YGGCCR | 1 cut(s) | 857 |
| AcsI | RAATTY | 3 cut(s) | 488, 642, 1883 |
| AcuI | CTGAAG | 5 cut(s) | 1094, 1238, 1711, 1890, 2157 |
| AdeI | CACNNNGTG | 2 cut(s) | 849, 2246 |
| AfaI | GTAC | 6 cut(s) | 842, 870, 1012, 1801, 1809, 1929 |
| AfeI | AGCGCT | 2 cut(s) | 1093, 2275 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1663, 1763, 2166 |
| AflII | CTTAAG | 1 cut(s) | 647 |
| AflIII | ACRYGT | 2 cut(s) | 1018, 1443 |
| AgsI | TTSAA | 8 cut(s) | 455, 493, 697, 882, 1103, 1225, 1826, 1979 |
| AjnI | CCWGG | 1 cut(s) | 340 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 189, 221 |
| AleI | CACNNNNGTG | 1 cut(s) | 688 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1702, 1734 |
| Alw21I | GWGCWC | 3 cut(s) | 672, 2209, 2242 |
| Alw26I | GTCTC | 6 cut(s) | 41, 1006, 1230, 1515, 2027, 2214 |
| Alw44I | GTGCAC | 1 cut(s) | 2238 |
| AlwI | GGATC | 5 cut(s) | 322, 564, 911, 924, 945 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2259 |
| Aor51HI | AGCGCT | 2 cut(s) | 1093, 2275 |
| AoxI | GGCC | 6 cut(s) | 6, 101, 338, 857, 1568, 1622 |
| ApaLI | GTGCAC | 1 cut(s) | 2238 |
| ApeKI | GCWGC | 4 cut(s) | 185, 210, 1157, 1471 |
| ApoI | RAATTY | 3 cut(s) | 488, 642, 1883 |
| AseI | ATTAAT | 1 cut(s) | 1599 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 201 |
| Asp718I | GGTACC | 2 cut(s) | 1799, 1807 |
| AspA2I | CCTAGG | 1 cut(s) | 1433 |
| AspLEI | GCGC | 4 cut(s) | 717, 1094, 1306, 2276 |
| AspS9I | GGNCC | 6 cut(s) | 101, 331, 338, 371, 1568, 2256 |
| AsuC2I | CCSGG | 1 cut(s) | 100 |
| AsuHPI | GGTGA | 2 cut(s) | 250, 1210 |
| AsuII | TTCGAA | 1 cut(s) | 610 |
| AvaII | GGWCC | 3 cut(s) | 331, 371, 2256 |
| AvrII | CCTAGG | 1 cut(s) | 1433 |
| AxyI | CCTNAGG | 1 cut(s) | 933 |
| BaeGI | GKGCMC | 1 cut(s) | 2242 |
| BaeI | ACNNNNGTAYC | 6 cut(s) | 1791, 1791, 1824, 1824, 1919, 1952 |
| BalI | TGGCCA | 1 cut(s) | 859 |
| BamHI | GGATCC | 1 cut(s) | 916 |
| BanI | GGYRCC | 2 cut(s) | 1799, 1807 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 435, 467 |
| BbsI | GAAGAC | 2 cut(s) | 27, 1480 |
| Bbv12I | GWGCWC | 3 cut(s) | 672, 2209, 2242 |
| BbvI | GCAGC | 4 cut(s) | 172, 197, 1169, 1483 |
| BccI | CCATC | 6 cut(s) | 271, 382, 1269, 1432, 1490, 1853 |
| BceAI | ACGGC | 2 cut(s) | 88, 88 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 98, 132, 590, 624 |
| BciT130I | CCWGG | 1 cut(s) | 342 |
| BciVI | GTATCC | 2 cut(s) | 1278, 1687 |
| BcnI | CCSGG | 1 cut(s) | 100 |
| BcoDI | GTCTC | 6 cut(s) | 41, 1006, 1230, 1515, 2027, 2214 |
| BfaI | CTAG | 6 cut(s) | 810, 990, 1434, 1634, 1722, 2328 |
| BfmI | CTRYAG | 2 cut(s) | 1139, 1890 |
| BfoI | RGCGCY | 2 cut(s) | 1095, 2277 |
| BfrI | CTTAAG | 1 cut(s) | 647 |
| BfuAI | ACCTGC | 2 cut(s) | 1132, 2244 |
| BfuI | GTATCC | 2 cut(s) | 1278, 1687 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 261 |
| BisI | GCNGC | 6 cut(s) | 6, 59, 186, 211, 1158, 1472 |
| BlnI | CCTAGG | 1 cut(s) | 1433 |
| BlsI | GCNGC | 6 cut(s) | 7, 60, 187, 212, 1159, 1473 |
| BmcAI | AGTACT | 1 cut(s) | 1012 |
| Bme1390I | CCNGG | 2 cut(s) | 100, 342 |
| Bme18I | GGWCC | 3 cut(s) | 331, 371, 2256 |
| BmgT120I | GGNCC | 6 cut(s) | 101, 331, 338, 371, 1568, 2256 |
| BmiI | GGNNCC | 5 cut(s) | 332, 918, 1801, 1809, 2171 |
| BmrFI | CCNGG | 2 cut(s) | 100, 342 |
| BmrI | ACTGGG | 2 cut(s) | 244, 473 |
| BmsI | GCATC | 5 cut(s) | 84, 277, 457, 1153, 1298 |
| BmuI | ACTGGG | 2 cut(s) | 244, 473 |
| BpiI | GAAGAC | 2 cut(s) | 27, 1480 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2024, 2056 |
| BpmI | CTGGAG | 2 cut(s) | 363, 1782 |
| Bpu14I | TTCGAA | 1 cut(s) | 610 |
| BpuEI | CTTGAG | 1 cut(s) | 122 |
| BpuMI | CCSGG | 1 cut(s) | 100 |
| BsaAI | YACGTR | 2 cut(s) | 1019, 1379 |
| BsaI | GGTCTC | 1 cut(s) | 1515 |
| BsaJI | CCNNGG | 5 cut(s) | 334, 1042, 1433, 1803, 1959 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2211, 2241 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1663, 1763, 2166 |
| Bse1I | ACTGG | 7 cut(s) | 239, 479, 742, 1084, 1765, 2050, 2239 |
| Bse21I | CCTNAGG | 1 cut(s) | 933 |
| Bse3DI | GCAATG | 1 cut(s) | 595 |
| BseBI | CCWGG | 1 cut(s) | 342 |
| BseDI | CCNNGG | 5 cut(s) | 334, 1042, 1433, 1803, 1959 |
| BseGI | GGATG | 7 cut(s) | 292, 420, 1261, 1289, 1443, 1930, 2191 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1663, 1763, 2166 |
| BseMI | GCAATG | 1 cut(s) | 595 |
| BseMII | CTCAG | 6 cut(s) | 36, 582, 700, 860, 947, 1017 |
| BseNI | ACTGG | 7 cut(s) | 239, 479, 742, 1084, 1765, 2050, 2239 |
| BseRI | GAGGAG | 2 cut(s) | 407, 1076 |
| BseSI | GKGCMC | 1 cut(s) | 2242 |
| BseXI | GCAGC | 4 cut(s) | 172, 197, 1169, 1483 |
| BsgI | GTGCAG | 1 cut(s) | 2245 |
| Bsh1236I | CGCG | 1 cut(s) | 717 |
| BshFI | GGCC | 6 cut(s) | 8, 103, 340, 859, 1570, 1624 |
| BshNI | GGYRCC | 2 cut(s) | 1799, 1807 |
| BsiHKAI | GWGCWC | 3 cut(s) | 672, 2209, 2242 |
| BsiSI | CCGG | 2 cut(s) | 99, 1465 |
| BslFI | GGGAC | 3 cut(s) | 461, 533, 2003 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1663, 1763, 2166 |
| BsmAI | GTCTC | 6 cut(s) | 41, 1006, 1230, 1515, 2027, 2214 |
| BsmFI | GGGAC | 3 cut(s) | 461, 533, 2003 |
| BsmI | GAATGC | 2 cut(s) | 1254, 2137 |
| BsnI | GGCC | 6 cut(s) | 8, 103, 340, 859, 1570, 1624 |
| Bso31I | GGTCTC | 1 cut(s) | 1515 |
| Bsp119I | TTCGAA | 1 cut(s) | 610 |
| Bsp1286I | GDGCHC | 3 cut(s) | 672, 2209, 2242 |
| Bsp1407I | TGTACA | 1 cut(s) | 840 |
| Bsp143I | GATC | 8 cut(s) | 115, 271, 314, 514, 556, 637, 916, 937 |
| Bsp19I | CCATGG | 3 cut(s) | 334, 1042, 1803 |
| BspACI | CCGC | 5 cut(s) | 5, 59, 717, 1694, 1837 |
| BspANI | GGCC | 6 cut(s) | 8, 103, 340, 859, 1570, 1624 |
| BspCNI | CTCAG | 7 cut(s) | 4, 37, 581, 699, 859, 946, 1016 |
| BspFNI | CGCG | 1 cut(s) | 717 |
| BspHI | TCATGA | 1 cut(s) | 483 |
| BspLI | GGNNCC | 5 cut(s) | 332, 918, 1801, 1809, 2171 |
| BspMAI | CTGCAG | 1 cut(s) | 1143 |
| BspMI | ACCTGC | 2 cut(s) | 1132, 2244 |
| BspPI | GGATC | 5 cut(s) | 322, 564, 911, 924, 945 |
| BspT104I | TTCGAA | 1 cut(s) | 610 |
| BspT107I | GGYRCC | 2 cut(s) | 1799, 1807 |
| BspTI | CTTAAG | 1 cut(s) | 647 |
| BspTNI | GGTCTC | 1 cut(s) | 1515 |
| BsrDI | GCAATG | 1 cut(s) | 595 |
| BsrGI | TGTACA | 1 cut(s) | 840 |
| BsrI | ACTGG | 7 cut(s) | 239, 479, 742, 1084, 1765, 2050, 2239 |
| BssECI | CCNNGG | 5 cut(s) | 334, 1042, 1433, 1803, 1959 |
| BssMI | GATC | 8 cut(s) | 115, 271, 314, 514, 556, 637, 916, 937 |
| BssNAI | GTATAC | 1 cut(s) | 1281 |
| BssT1I | CCWWGG | 5 cut(s) | 334, 1042, 1433, 1803, 1959 |
| Bst1107I | GTATAC | 1 cut(s) | 1281 |
| Bst2UI | CCWGG | 1 cut(s) | 342 |
| Bst4CI | ACNGT | 5 cut(s) | 607, 1387, 1509, 1894, 2293 |
| Bst6I | CTCTTC | 4 cut(s) | 192, 432, 882, 1905 |
| BstAFI | CTTAAG | 1 cut(s) | 647 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1163, 2259 |
| BstAUI | TGTACA | 1 cut(s) | 840 |
| BstBAI | YACGTR | 2 cut(s) | 1019, 1379 |
| BstBI | TTCGAA | 1 cut(s) | 610 |
| BstC8I | GCNNGC | 2 cut(s) | 529, 1535 |
| BstDEI | CTNAG | 8 cut(s) | 12, 45, 568, 686, 846, 933, 1003, 1208 |
| BstDSI | CCRYGG | 3 cut(s) | 334, 1042, 1803 |
| BstEII | GGTNACC | 1 cut(s) | 903 |
| BstF5I | GGATG | 7 cut(s) | 292, 420, 1261, 1289, 1443, 1930, 2191 |
| BstFNI | CGCG | 1 cut(s) | 717 |
| BstH2I | RGCGCY | 2 cut(s) | 1095, 2277 |
| BstHHI | GCGC | 4 cut(s) | 717, 1094, 1306, 2276 |
| BstKTI | GATC | 8 cut(s) | 118, 274, 317, 517, 559, 640, 919, 940 |
| BstMAI | GTCTC | 6 cut(s) | 41, 1006, 1230, 1515, 2027, 2214 |
| BstMBI | GATC | 8 cut(s) | 115, 271, 314, 514, 556, 637, 916, 937 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 261, 714, 1089, 1163, 1213, 1310, 1874, 2259 |
| BstNI | CCWGG | 1 cut(s) | 342 |
| BstNSI | RCATGY | 2 cut(s) | 1447, 1971 |
| BstPI | GGTNACC | 1 cut(s) | 903 |
| BstSCI | CCNGG | 2 cut(s) | 98, 340 |
| BstSFI | CTRYAG | 2 cut(s) | 1139, 1890 |
| BstSLI | GKGCMC | 1 cut(s) | 2242 |
| BstUI | CGCG | 1 cut(s) | 717 |
| BstV1I | GCAGC | 4 cut(s) | 172, 197, 1169, 1483 |
| BstV2I | GAAGAC | 2 cut(s) | 27, 1480 |
| BstX2I | RGATCY | 2 cut(s) | 556, 916 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 341 |
| BstYI | RGATCY | 2 cut(s) | 556, 916 |
| BstZ17I | GTATAC | 1 cut(s) | 1281 |
| Bsu36I | CCTNAGG | 1 cut(s) | 933 |
| BsuI | GTATCC | 2 cut(s) | 1278, 1687 |
| BsuRI | GGCC | 6 cut(s) | 8, 103, 340, 859, 1570, 1624 |
| BtgI | CCRYGG | 3 cut(s) | 334, 1042, 1803 |
| BtgZI | GCGATG | 1 cut(s) | 1831 |
| BtsCI | GGATG | 7 cut(s) | 292, 420, 1261, 1289, 1443, 1930, 2191 |
| BtsIMutI | CAGTG | 3 cut(s) | 854, 1091, 1262 |
| BveI | ACCTGC | 2 cut(s) | 1132, 2244 |
| Cac8I | GCNNGC | 2 cut(s) | 529, 1535 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2259 |
| CciI | TCATGA | 1 cut(s) | 483 |
| CfoI | GCGC | 4 cut(s) | 717, 1094, 1306, 2276 |
| Cfr13I | GGNCC | 6 cut(s) | 101, 331, 338, 371, 1568, 2256 |
| CseI | GACGC | 1 cut(s) | 1295 |
| Csp6I | GTAC | 6 cut(s) | 841, 869, 1011, 1800, 1808, 1928 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1756, 1791 |
| CviQI | GTAC | 6 cut(s) | 841, 869, 1011, 1800, 1808, 1928 |
| DdeI | CTNAG | 8 cut(s) | 12, 45, 568, 686, 846, 933, 1003, 1208 |
| DpnI | GATC | 8 cut(s) | 117, 273, 316, 516, 558, 639, 918, 939 |
| DpnII | GATC | 8 cut(s) | 115, 271, 314, 514, 556, 637, 916, 937 |
| DraIII | CACNNNGTG | 2 cut(s) | 849, 2246 |
| EaeI | YGGCCR | 1 cut(s) | 857 |
| Eam1104I | CTCTTC | 4 cut(s) | 192, 432, 882, 1905 |
| EarI | CTCTTC | 4 cut(s) | 192, 432, 882, 1905 |
| Eco130I | CCWWGG | 5 cut(s) | 334, 1042, 1433, 1803, 1959 |
| Eco31I | GGTCTC | 1 cut(s) | 1515 |
| Eco47I | GGWCC | 3 cut(s) | 331, 371, 2256 |
| Eco47III | AGCGCT | 2 cut(s) | 1093, 2275 |
| Eco57I | CTGAAG | 5 cut(s) | 1094, 1238, 1711, 1890, 2157 |
| Eco81I | CCTNAGG | 1 cut(s) | 933 |
| Eco91I | GGTNACC | 1 cut(s) | 903 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2256 |
| EcoO65I | GGTNACC | 1 cut(s) | 903 |
| EcoRI | GAATTC | 1 cut(s) | 1883 |
| EcoRII | CCWGG | 1 cut(s) | 340 |
| EcoT14I | CCWWGG | 5 cut(s) | 334, 1042, 1433, 1803, 1959 |
| ErhI | CCWWGG | 5 cut(s) | 334, 1042, 1433, 1803, 1959 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 131, 163, 189, 221 |
| FaqI | GGGAC | 3 cut(s) | 461, 533, 2003 |
| FauI | CCCGC | 2 cut(s) | 1687, 1830 |
| FauNDI | CATATG | 3 cut(s) | 379, 951, 2302 |
| FblI | GTMKAC | 2 cut(s) | 1280, 1753 |
| Fnu4HI | GCNGC | 6 cut(s) | 6, 59, 186, 211, 1158, 1472 |
| FokI | GGATG | 7 cut(s) | 299, 407, 1248, 1276, 1450, 1917, 2198 |
| Fsp4HI | GCNGC | 6 cut(s) | 6, 59, 186, 211, 1158, 1472 |
| FspAI | RTGCGCAY | 1 cut(s) | 1305 |
| FspBI | CTAG | 6 cut(s) | 810, 990, 1434, 1634, 1722, 2328 |
| FspI | TGCGCA | 1 cut(s) | 1305 |
| GlaI | GCGC | 4 cut(s) | 716, 1093, 1305, 2275 |
| GluI | GCNGC | 6 cut(s) | 6, 59, 186, 211, 1158, 1472 |
| GsuI | CTGGAG | 2 cut(s) | 363, 1782 |
| HaeII | RGCGCY | 2 cut(s) | 1095, 2277 |
| HaeIII | GGCC | 6 cut(s) | 8, 103, 340, 859, 1570, 1624 |
| HapII | CCGG | 2 cut(s) | 99, 1465 |
| HgaI | GACGC | 1 cut(s) | 1295 |
| HhaI | GCGC | 4 cut(s) | 717, 1094, 1306, 2276 |
| Hin6I | GCGC | 4 cut(s) | 715, 1092, 1304, 2274 |
| HinP1I | GCGC | 4 cut(s) | 715, 1092, 1304, 2274 |
| HincII | GTYRAC | 3 cut(s) | 122, 595, 2086 |
| HindII | GTYRAC | 3 cut(s) | 122, 595, 2086 |
| HindIII | AAGCTT | 1 cut(s) | 650 |
| HpaII | CCGG | 2 cut(s) | 99, 1465 |
| HphI | GGTGA | 2 cut(s) | 250, 1210 |
| Hpy99I | CGWCG | 1 cut(s) | 109 |
| HpyAV | CCTTC | 7 cut(s) | 1180, 1334, 1352, 1538, 1789, 1914, 2132 |
| HpyCH4III | ACNGT | 5 cut(s) | 607, 1387, 1509, 1894, 2293 |
| HpyCH4IV | ACGT | 2 cut(s) | 1018, 1378 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 261, 714, 1089, 1163, 1213, 1310, 1874, 2259 |
| HpyF3I | CTNAG | 8 cut(s) | 12, 45, 568, 686, 846, 933, 1003, 1208 |
| HpySE526I | ACGT | 2 cut(s) | 1018, 1378 |
| HspAI | GCGC | 4 cut(s) | 715, 1092, 1304, 2274 |
| KpnI | GGTACC | 2 cut(s) | 1803, 1811 |
| Kzo9I | GATC | 8 cut(s) | 115, 271, 314, 514, 556, 637, 916, 937 |
| LmnI | GCTCC | 4 cut(s) | 344, 1089, 2204, 2324 |
| Lsp1109I | GCAGC | 4 cut(s) | 172, 197, 1169, 1483 |
| LweI | GCATC | 5 cut(s) | 84, 277, 457, 1153, 1298 |
| MaeI | CTAG | 6 cut(s) | 810, 990, 1434, 1634, 1722, 2328 |
| MaeII | ACGT | 2 cut(s) | 1018, 1378 |
| MaeIII | GTNAC | 4 cut(s) | 442, 733, 903, 992 |
| MalI | GATC | 8 cut(s) | 117, 273, 316, 516, 558, 639, 918, 939 |
| MboI | GATC | 8 cut(s) | 115, 271, 314, 514, 556, 637, 916, 937 |
| MfeI | CAATTG | 4 cut(s) | 245, 510, 1781, 2176 |
| MflI | RGATCY | 2 cut(s) | 556, 916 |
| MhlI | GDGCHC | 3 cut(s) | 672, 2209, 2242 |
| MlsI | TGGCCA | 1 cut(s) | 859 |
| MluNI | TGGCCA | 1 cut(s) | 859 |
| MlyI | GAGTC | 5 cut(s) | 25, 43, 1679, 1850, 2033 |
| MmeI | TCCRAC | 3 cut(s) | 349, 1978, 2197 |
| Mox20I | TGGCCA | 1 cut(s) | 859 |
| MroXI | GAANNNNTTC | 1 cut(s) | 201 |
| MscI | TGGCCA | 1 cut(s) | 859 |
| MseI | TTAA | 3 cut(s) | 648, 1599, 2147 |
| MslI | CAYNNNNRTG | 2 cut(s) | 688, 2247 |
| Msp20I | TGGCCA | 1 cut(s) | 859 |
| MspCI | CTTAAG | 1 cut(s) | 647 |
| MspI | CCGG | 2 cut(s) | 99, 1465 |
| MspR9I | CCNGG | 2 cut(s) | 100, 342 |
| MunI | CAATTG | 4 cut(s) | 245, 510, 1781, 2176 |
| Mva1269I | GAATGC | 2 cut(s) | 1254, 2137 |
| MvaI | CCWGG | 1 cut(s) | 342 |
| MvnI | CGCG | 1 cut(s) | 717 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 261, 714, 1089, 1163, 1213, 1310, 1874, 2259 |
| NciI | CCSGG | 1 cut(s) | 100 |
| NcoI | CCATGG | 3 cut(s) | 334, 1042, 1803 |
| NdeI | CATATG | 3 cut(s) | 379, 951, 2302 |
| NdeII | GATC | 8 cut(s) | 115, 271, 314, 514, 556, 637, 916, 937 |
| NlaIV | GGNNCC | 5 cut(s) | 332, 918, 1801, 1809, 2171 |
| NmeAIII | GCCGAG | 3 cut(s) | 87, 1183, 1650 |
| NsbI | TGCGCA | 1 cut(s) | 1305 |
| NspI | RCATGY | 2 cut(s) | 1447, 1971 |
| NspV | TTCGAA | 1 cut(s) | 610 |
| OliI | CACNNNNGTG | 1 cut(s) | 688 |
| PagI | TCATGA | 1 cut(s) | 483 |
| PaqCI | CACCTGC | 1 cut(s) | 1132 |
| PciI | ACATGT | 1 cut(s) | 1443 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 38 |
| PctI | GAATGC | 2 cut(s) | 1254, 2137 |
| PdmI | GAANNNNTTC | 1 cut(s) | 201 |
| PfeI | GAWTC | 6 cut(s) | 155, 385, 575, 1199, 1859, 1955 |
| PkrI | GCNGC | 6 cut(s) | 7, 60, 187, 212, 1159, 1473 |
| PleI | GAGTC | 5 cut(s) | 24, 43, 1678, 1849, 2033 |
| PpsI | GAGTC | 5 cut(s) | 24, 43, 1678, 1849, 2033 |
| Ppu21I | YACGTR | 2 cut(s) | 1019, 1379 |
| PpuMI | RGGWCCY | 1 cut(s) | 2256 |
| PscI | ACATGT | 1 cut(s) | 1443 |
| PshBI | ATTAAT | 1 cut(s) | 1599 |
| Psp5II | RGGWCCY | 1 cut(s) | 2256 |
| Psp6I | CCWGG | 1 cut(s) | 340 |
| PspEI | GGTNACC | 1 cut(s) | 903 |
| PspGI | CCWGG | 1 cut(s) | 340 |
| PspN4I | GGNNCC | 5 cut(s) | 332, 918, 1801, 1809, 2171 |
| PspPI | GGNCC | 6 cut(s) | 101, 331, 338, 371, 1568, 2256 |
| PspPPI | RGGWCCY | 1 cut(s) | 2256 |
| PsrI | GAACNNNNNNTAC | 4 cut(s) | 888, 920, 977, 1009 |
| PstI | CTGCAG | 1 cut(s) | 1143 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2259 |
| PsuI | RGATCY | 2 cut(s) | 556, 916 |
| RsaI | GTAC | 6 cut(s) | 842, 870, 1012, 1801, 1809, 1929 |
| RsaNI | GTAC | 6 cut(s) | 841, 869, 1011, 1800, 1808, 1928 |
| RseI | CAYNNNNRTG | 2 cut(s) | 688, 2247 |
| SaqAI | TTAA | 3 cut(s) | 648, 1599, 2147 |
| SatI | GCNGC | 6 cut(s) | 6, 59, 186, 211, 1158, 1472 |
| Sau3AI | GATC | 8 cut(s) | 115, 271, 314, 514, 556, 637, 916, 937 |
| Sau96I | GGNCC | 6 cut(s) | 101, 331, 338, 371, 1568, 2256 |
| ScaI | AGTACT | 1 cut(s) | 1012 |
| SchI | GAGTC | 5 cut(s) | 25, 43, 1679, 1850, 2033 |
| ScrFI | CCNGG | 2 cut(s) | 100, 342 |
| SduI | GDGCHC | 3 cut(s) | 672, 2209, 2242 |
| SfaNI | GCATC | 5 cut(s) | 84, 277, 457, 1153, 1298 |
| SfcI | CTRYAG | 2 cut(s) | 1139, 1890 |
| SfuI | TTCGAA | 1 cut(s) | 610 |
| SinI | GGWCC | 3 cut(s) | 331, 371, 2256 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 688, 2247 |
| SmlI | CTYRAG | 2 cut(s) | 137, 647 |
| SmoI | CTYRAG | 2 cut(s) | 137, 647 |
| SsiI | CCGC | 5 cut(s) | 5, 59, 717, 1694, 1837 |
| SspMI | CTAG | 6 cut(s) | 810, 990, 1434, 1634, 1722, 2328 |
| StyD4I | CCNGG | 2 cut(s) | 98, 340 |
| StyI | CCWWGG | 5 cut(s) | 334, 1042, 1433, 1803, 1959 |
| TaaI | ACNGT | 5 cut(s) | 607, 1387, 1509, 1894, 2293 |
| TaiI | ACGT | 2 cut(s) | 1021, 1381 |
| TaqI | TCGA | 5 cut(s) | 132, 388, 610, 1119, 1513 |
| TatI | WGTACW | 3 cut(s) | 840, 868, 1010 |
| TauI | GCSGC | 2 cut(s) | 8, 61 |
| TfiI | GAWTC | 6 cut(s) | 155, 385, 575, 1199, 1859, 1955 |
| Tru1I | TTAA | 3 cut(s) | 648, 1599, 2147 |
| Tru9I | TTAA | 3 cut(s) | 648, 1599, 2147 |
| TscAI | CASTG | 3 cut(s) | 854, 1091, 1262 |
| TseI | GCWGC | 4 cut(s) | 185, 210, 1157, 1471 |
| TspDTI | ATGAA | 9 cut(s) | 500, 588, 909, 935, 998, 1557, 1569, 1872, 2187 |
| TspGWI | ACGGA | 2 cut(s) | 1254, 1923 |
| TspRI | CASTG | 3 cut(s) | 854, 1091, 1262 |
| Vha464I | CTTAAG | 1 cut(s) | 647 |
| VneI | GTGCAC | 1 cut(s) | 2238 |
| VpaK11BI | GGWCC | 3 cut(s) | 331, 371, 2256 |
| VspI | ATTAAT | 1 cut(s) | 1599 |
| XapI | RAATTY | 3 cut(s) | 488, 642, 1883 |
| XceI | RCATGY | 2 cut(s) | 1447, 1971 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1810 |
| XmaJI | CCTAGG | 1 cut(s) | 1433 |
| XmiI | GTMKAC | 2 cut(s) | 1280, 1753 |
| XmnI | GAANNNNTTC | 1 cut(s) | 201 |
| XspI | CTAG | 6 cut(s) | 810, 990, 1434, 1634, 1722, 2328 |
| ZrmI | AGTACT | 1 cut(s) | 1012 |