Poly(A) polymerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1649 |
| Acc36I | ACCTGC | 1 cut(s) | 1544 |
| AccI | GTMKAC | 2 cut(s) | 830, 2031 |
| AccIII | TCCGGA | 1 cut(s) | 663 |
| AciI | CCGC | 5 cut(s) | 47, 199, 928, 2140, 2199 |
| AclWI | GGATC | 6 cut(s) | 11, 24, 267, 571, 1576, 1873 |
| AcoI | YGGCCR | 2 cut(s) | 196, 845 |
| AcsI | RAATTY | 2 cut(s) | 476, 630 |
| AcuI | CTGAAG | 1 cut(s) | 1702 |
| AcyI | GRCGYC | 1 cut(s) | 1646 |
| AdeI | CACNNNGTG | 1 cut(s) | 351 |
| AfaI | GTAC | 3 cut(s) | 1000, 1406, 2173 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 901, 2149, 2208 |
| AflIII | ACRYGT | 2 cut(s) | 1401, 1407 |
| AgsI | TTSAA | 9 cut(s) | 110, 443, 481, 685, 717, 870, 875, 1091, 1213 |
| AjiI | CACGTC | 1 cut(s) | 704 |
| AjnI | CCWGG | 6 cut(s) | 328, 411, 486, 1420, 1586, 1820 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 13, 45, 177, 209 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 876, 908 |
| Alw21I | GWGCWC | 5 cut(s) | 660, 1084, 1705, 1957, 2064 |
| Alw26I | GTCTC | 2 cut(s) | 994, 1218 |
| AlwI | GGATC | 6 cut(s) | 11, 24, 267, 571, 1576, 1873 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 452 |
| Ama87I | CYCGRG | 1 cut(s) | 921 |
| Aor13HI | TCCGGA | 1 cut(s) | 663 |
| AoxI | GGCC | 8 cut(s) | 196, 326, 359, 845, 1068, 1622, 1677, 1877 |
| ApeKI | GCWGC | 5 cut(s) | 173, 612, 1145, 1240, 1459 |
| ApoI | RAATTY | 2 cut(s) | 476, 630 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2024, 2056 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 189 |
| AspA2I | CCTAGG | 1 cut(s) | 2155 |
| AspLEI | GCGC | 1 cut(s) | 1301 |
| AsuC2I | CCSGG | 2 cut(s) | 35, 1192 |
| AsuHPI | GGTGA | 3 cut(s) | 238, 1965, 2015 |
| AsuII | TTCGAA | 1 cut(s) | 598 |
| AvaI | CYCGRG | 1 cut(s) | 921 |
| AvaII | GGWCC | 7 cut(s) | 89, 904, 925, 2137, 2152, 2196, 2211 |
| AvrII | CCTAGG | 1 cut(s) | 2155 |
| AxyI | CCTNAGG | 1 cut(s) | 1260 |
| BalI | TGGCCA | 1 cut(s) | 847 |
| BamHI | GGATCC | 1 cut(s) | 16 |
| BanII | GRGCYC | 1 cut(s) | 1957 |
| Bbv12I | GWGCWC | 5 cut(s) | 660, 1084, 1705, 1957, 2064 |
| BbvI | GCAGC | 5 cut(s) | 160, 599, 1157, 1227, 1471 |
| BccI | CCATC | 8 cut(s) | 259, 370, 1173, 1257, 1420, 1478, 1660, 1989 |
| BceAI | ACGGC | 3 cut(s) | 76, 625, 2062 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 578, 612, 2038, 2072 |
| BciT130I | CCWGG | 6 cut(s) | 330, 413, 488, 1422, 1588, 1822 |
| BciVI | GTATCC | 1 cut(s) | 1819 |
| BclI | TGATCA | 3 cut(s) | 205, 1600, 1774 |
| BcnI | CCSGG | 2 cut(s) | 35, 1192 |
| BcoDI | GTCTC | 2 cut(s) | 994, 1218 |
| BfaI | CTAG | 7 cut(s) | 798, 978, 1634, 1682, 1851, 2156, 2215 |
| BfmI | CTRYAG | 3 cut(s) | 613, 1241, 1551 |
| BfuAI | ACCTGC | 1 cut(s) | 1544 |
| BfuI | GTATCC | 1 cut(s) | 1819 |
| BglII | AGATCT | 2 cut(s) | 2145, 2204 |
| BisI | GCNGC | 7 cut(s) | 47, 174, 199, 613, 1146, 1241, 1460 |
| BlnI | CCTAGG | 1 cut(s) | 2155 |
| BlsI | GCNGC | 7 cut(s) | 48, 175, 200, 614, 1147, 1242, 1461 |
| BmcAI | AGTACT | 1 cut(s) | 1000 |
| Bme1390I | CCNGG | 8 cut(s) | 35, 330, 413, 488, 1192, 1422, 1588, 1822 |
| Bme18I | GGWCC | 7 cut(s) | 89, 904, 925, 2137, 2152, 2196, 2211 |
| BmeT110I | CYCGRG | 1 cut(s) | 921 |
| BmgBI | CACGTC | 1 cut(s) | 704 |
| BmrFI | CCNGG | 8 cut(s) | 35, 330, 413, 488, 1192, 1422, 1588, 1822 |
| BmrI | ACTGGG | 1 cut(s) | 1066 |
| BmsI | GCATC | 7 cut(s) | 35, 72, 265, 445, 1227, 1747, 2015 |
| BmuI | ACTGGG | 1 cut(s) | 1066 |
| BpmI | CTGGAG | 2 cut(s) | 351, 1773 |
| Bpu14I | TTCGAA | 1 cut(s) | 598 |
| BpuMI | CCSGG | 2 cut(s) | 35, 1192 |
| Bsa29I | ATCGAT | 1 cut(s) | 501 |
| BsaAI | YACGTR | 2 cut(s) | 1367, 1408 |
| BsaHI | GRCGYC | 1 cut(s) | 1646 |
| BsaWI | WCCGGW | 1 cut(s) | 663 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2087, 2117 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 901, 2149, 2208 |
| Bse1I | ACTGG | 4 cut(s) | 446, 730, 1072, 1756 |
| Bse21I | CCTNAGG | 1 cut(s) | 1260 |
| BseAI | TCCGGA | 1 cut(s) | 663 |
| BseBI | CCWGG | 6 cut(s) | 330, 413, 488, 1422, 1588, 1822 |
| BseCI | ATCGAT | 1 cut(s) | 501 |
| BseGI | GGATG | 6 cut(s) | 280, 421, 1184, 1242, 1249, 1431 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 901, 2149, 2208 |
| BseMII | CTCAG | 3 cut(s) | 688, 848, 1005 |
| BseNI | ACTGG | 4 cut(s) | 446, 730, 1072, 1756 |
| BseRI | GAGGAG | 1 cut(s) | 1968 |
| BseXI | GCAGC | 5 cut(s) | 160, 599, 1157, 1227, 1471 |
| BseYI | CCCAGC | 1 cut(s) | 222 |
| BshFI | GGCC | 8 cut(s) | 198, 328, 361, 847, 1070, 1624, 1679, 1879 |
| BshVI | ATCGAT | 1 cut(s) | 501 |
| BsiHKAI | GWGCWC | 5 cut(s) | 660, 1084, 1705, 1957, 2064 |
| BsiHKCI | CYCGRG | 1 cut(s) | 921 |
| BsiSI | CCGG | 4 cut(s) | 35, 664, 1192, 1453 |
| BslFI | GGGAC | 5 cut(s) | 75, 449, 629, 917, 938 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 901, 2149, 2208 |
| BsmAI | GTCTC | 2 cut(s) | 994, 1218 |
| BsmFI | GGGAC | 5 cut(s) | 75, 449, 629, 917, 938 |
| BsmI | GAATGC | 2 cut(s) | 286, 1909 |
| BsnI | GGCC | 8 cut(s) | 198, 328, 361, 847, 1070, 1624, 1679, 1879 |
| BsoBI | CYCGRG | 1 cut(s) | 921 |
| Bsp119I | TTCGAA | 1 cut(s) | 598 |
| Bsp1286I | GDGCHC | 5 cut(s) | 660, 1084, 1705, 1957, 2064 |
| Bsp13I | TCCGGA | 1 cut(s) | 663 |
| Bsp19I | CCATGG | 1 cut(s) | 1030 |
| BspACI | CCGC | 5 cut(s) | 47, 199, 928, 2140, 2199 |
| BspANI | GGCC | 8 cut(s) | 198, 328, 361, 847, 1070, 1624, 1679, 1879 |
| BspCNI | CTCAG | 3 cut(s) | 687, 847, 1004 |
| BspDI | ATCGAT | 1 cut(s) | 501 |
| BspEI | TCCGGA | 1 cut(s) | 663 |
| BspHI | TCATGA | 1 cut(s) | 471 |
| BspMAI | CTGCAG | 3 cut(s) | 617, 1245, 1555 |
| BspMI | ACCTGC | 1 cut(s) | 1544 |
| BspPI | GGATC | 6 cut(s) | 11, 24, 267, 571, 1576, 1873 |
| BspT104I | TTCGAA | 1 cut(s) | 598 |
| BsrI | ACTGG | 4 cut(s) | 446, 730, 1072, 1756 |
| BssNAI | GTATAC | 1 cut(s) | 831 |
| BssNI | GRCGYC | 1 cut(s) | 1646 |
| BssT1I | CCWWGG | 3 cut(s) | 733, 1030, 2155 |
| Bst1107I | GTATAC | 1 cut(s) | 831 |
| Bst2UI | CCWGG | 6 cut(s) | 330, 413, 488, 1422, 1588, 1822 |
| Bst4CI | ACNGT | 6 cut(s) | 595, 1375, 1797, 1813, 1831, 1885 |
| Bst6I | CTCTTC | 6 cut(s) | 173, 180, 241, 420, 870, 1106 |
| BstACI | GRCGYC | 1 cut(s) | 1646 |
| BstBAI | YACGTR | 2 cut(s) | 1367, 1408 |
| BstBI | TTCGAA | 1 cut(s) | 598 |
| BstC8I | GCNNGC | 4 cut(s) | 517, 776, 1626, 1957 |
| BstDEI | CTNAG | 6 cut(s) | 674, 757, 834, 991, 1260, 2074 |
| BstDSI | CCRYGG | 1 cut(s) | 1030 |
| BstF5I | GGATG | 6 cut(s) | 280, 421, 1184, 1242, 1249, 1431 |
| BstHHI | GCGC | 1 cut(s) | 1301 |
| BstMAI | GTCTC | 2 cut(s) | 994, 1218 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 52, 1296 |
| BstNI | CCWGG | 6 cut(s) | 330, 413, 488, 1422, 1588, 1822 |
| BstNSI | RCATGY | 3 cut(s) | 805, 1405, 1517 |
| BstSCI | CCNGG | 8 cut(s) | 33, 328, 411, 486, 1190, 1420, 1586, 1820 |
| BstSFI | CTRYAG | 3 cut(s) | 613, 1241, 1551 |
| BstV1I | GCAGC | 5 cut(s) | 160, 599, 1157, 1227, 1471 |
| BstX2I | RGATCY | 6 cut(s) | 16, 563, 1568, 1865, 2145, 2204 |
| BstYI | RGATCY | 6 cut(s) | 16, 563, 1568, 1865, 2145, 2204 |
| BstZ17I | GTATAC | 1 cut(s) | 831 |
| Bsu15I | ATCGAT | 1 cut(s) | 501 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1260 |
| BsuI | GTATCC | 1 cut(s) | 1819 |
| BsuRI | GGCC | 8 cut(s) | 198, 328, 361, 847, 1070, 1624, 1679, 1879 |
| BsuTUI | ATCGAT | 1 cut(s) | 501 |
| BtgI | CCRYGG | 1 cut(s) | 1030 |
| BtgZI | GCGATG | 2 cut(s) | 1987, 2008 |
| BtrI | CACGTC | 1 cut(s) | 704 |
| BtsCI | GGATG | 6 cut(s) | 280, 421, 1184, 1242, 1249, 1431 |
| BtsI | GCAGTG | 2 cut(s) | 1250, 1294 |
| BtsIMutI | CAGTG | 5 cut(s) | 842, 1079, 1250, 1294, 1890 |
| BveI | ACCTGC | 1 cut(s) | 1544 |
| Cac8I | GCNNGC | 4 cut(s) | 517, 776, 1626, 1957 |
| CaiI | CAGNNNCTG | 1 cut(s) | 452 |
| CciI | TCATGA | 1 cut(s) | 471 |
| CfoI | GCGC | 1 cut(s) | 1301 |
| ClaI | ATCGAT | 1 cut(s) | 501 |
| CseI | GACGC | 1 cut(s) | 1484 |
| Csp6I | GTAC | 3 cut(s) | 999, 1405, 2172 |
| CviQI | GTAC | 3 cut(s) | 999, 1405, 2172 |
| DdeI | CTNAG | 6 cut(s) | 674, 757, 834, 991, 1260, 2074 |
| DraIII | CACNNNGTG | 1 cut(s) | 351 |
| EaeI | YGGCCR | 2 cut(s) | 196, 845 |
| Eam1104I | CTCTTC | 6 cut(s) | 173, 180, 241, 420, 870, 1106 |
| EarI | CTCTTC | 6 cut(s) | 173, 180, 241, 420, 870, 1106 |
| EciI | GGCGGA | 2 cut(s) | 2129, 2188 |
| Ecl136II | GAGCTC | 1 cut(s) | 1955 |
| Eco130I | CCWWGG | 3 cut(s) | 733, 1030, 2155 |
| Eco24I | GRGCYC | 1 cut(s) | 1957 |
| Eco32I | GATATC | 2 cut(s) | 409, 553 |
| Eco47I | GGWCC | 7 cut(s) | 89, 904, 925, 2137, 2152, 2196, 2211 |
| Eco53kI | GAGCTC | 1 cut(s) | 1955 |
| Eco57I | CTGAAG | 1 cut(s) | 1702 |
| Eco81I | CCTNAGG | 1 cut(s) | 1260 |
| Eco88I | CYCGRG | 1 cut(s) | 921 |
| EcoICRI | GAGCTC | 1 cut(s) | 1955 |
| EcoO109I | RGGNCCY | 4 cut(s) | 89, 904, 2152, 2211 |
| EcoRII | CCWGG | 6 cut(s) | 328, 411, 486, 1420, 1586, 1820 |
| EcoRV | GATATC | 2 cut(s) | 409, 553 |
| EcoT14I | CCWWGG | 3 cut(s) | 733, 1030, 2155 |
| EcoT22I | ATGCAT | 1 cut(s) | 28 |
| EcoT38I | GRGCYC | 1 cut(s) | 1957 |
| ErhI | CCWWGG | 3 cut(s) | 733, 1030, 2155 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 62, 94, 177, 209 |
| FaqI | GGGAC | 5 cut(s) | 75, 449, 629, 917, 938 |
| FauNDI | CATATG | 4 cut(s) | 367, 939, 2132, 2191 |
| FbaI | TGATCA | 3 cut(s) | 205, 1600, 1774 |
| FblI | GTMKAC | 2 cut(s) | 830, 2031 |
| Fnu4HI | GCNGC | 7 cut(s) | 47, 174, 199, 613, 1146, 1241, 1460 |
| FokI | GGATG | 6 cut(s) | 287, 428, 1191, 1236, 1249, 1438 |
| FriOI | GRGCYC | 1 cut(s) | 1957 |
| Fsp4HI | GCNGC | 7 cut(s) | 47, 174, 199, 613, 1146, 1241, 1460 |
| FspBI | CTAG | 7 cut(s) | 798, 978, 1634, 1682, 1851, 2156, 2215 |
| GlaI | GCGC | 1 cut(s) | 1300 |
| GluI | GCNGC | 7 cut(s) | 47, 174, 199, 613, 1146, 1241, 1460 |
| GsaI | CCCAGC | 1 cut(s) | 226 |
| GsuI | CTGGAG | 2 cut(s) | 351, 1773 |
| HaeIII | GGCC | 8 cut(s) | 198, 328, 361, 847, 1070, 1624, 1679, 1879 |
| HapII | CCGG | 4 cut(s) | 35, 664, 1192, 1453 |
| HgaI | GACGC | 1 cut(s) | 1484 |
| HhaI | GCGC | 1 cut(s) | 1301 |
| Hin1I | GRCGYC | 1 cut(s) | 1646 |
| Hin6I | GCGC | 1 cut(s) | 1299 |
| HinP1I | GCGC | 1 cut(s) | 1299 |
| HincII | GTYRAC | 3 cut(s) | 1273, 2032, 2092 |
| HindII | GTYRAC | 3 cut(s) | 1273, 2032, 2092 |
| HpaII | CCGG | 4 cut(s) | 35, 664, 1192, 1453 |
| HphI | GGTGA | 3 cut(s) | 238, 1965, 2015 |
| Hpy166II | GTNNAC | 6 cut(s) | 831, 1135, 1273, 1658, 2032, 2092 |
| Hpy8I | GTNNAC | 6 cut(s) | 831, 1135, 1273, 1658, 2032, 2092 |
| Hpy99I | CGWCG | 5 cut(s) | 2033, 2048, 2111, 2123, 2182 |
| HpyAV | CCTTC | 4 cut(s) | 941, 1168, 1340, 1385 |
| HpyCH4III | ACNGT | 6 cut(s) | 595, 1375, 1797, 1813, 1831, 1885 |
| HpyCH4IV | ACGT | 7 cut(s) | 703, 1169, 1275, 1366, 1407, 1437, 1646 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 52, 1296 |
| HpyF3I | CTNAG | 6 cut(s) | 674, 757, 834, 991, 1260, 2074 |
| HpySE526I | ACGT | 7 cut(s) | 703, 1169, 1275, 1366, 1407, 1437, 1646 |
| Hsp92I | GRCGYC | 1 cut(s) | 1646 |
| HspAI | GCGC | 1 cut(s) | 1299 |
| KflI | GGGWCCC | 2 cut(s) | 89, 904 |
| Kpn2I | TCCGGA | 1 cut(s) | 663 |
| Ksp22I | TGATCA | 3 cut(s) | 205, 1600, 1774 |
| LmnI | GCTCC | 2 cut(s) | 332, 1700 |
| Lsp1109I | GCAGC | 5 cut(s) | 160, 599, 1157, 1227, 1471 |
| LweI | GCATC | 7 cut(s) | 35, 72, 265, 445, 1227, 1747, 2015 |
| MaeI | CTAG | 7 cut(s) | 798, 978, 1634, 1682, 1851, 2156, 2215 |
| MaeII | ACGT | 7 cut(s) | 703, 1169, 1275, 1366, 1407, 1437, 1646 |
| MaeIII | GTNAC | 5 cut(s) | 430, 721, 980, 1276, 1897 |
| MfeI | CAATTG | 3 cut(s) | 233, 870, 1661 |
| MflI | RGATCY | 6 cut(s) | 16, 563, 1568, 1865, 2145, 2204 |
| MhlI | GDGCHC | 5 cut(s) | 660, 1084, 1705, 1957, 2064 |
| MlsI | TGGCCA | 1 cut(s) | 847 |
| MluNI | TGGCCA | 1 cut(s) | 847 |
| MlyI | GAGTC | 1 cut(s) | 1679 |
| MmeI | TCCRAC | 2 cut(s) | 1771, 2130 |
| Mox20I | TGGCCA | 1 cut(s) | 847 |
| Mph1103I | ATGCAT | 1 cut(s) | 28 |
| MroI | TCCGGA | 1 cut(s) | 663 |
| MroXI | GAANNNNTTC | 1 cut(s) | 189 |
| MscI | TGGCCA | 1 cut(s) | 847 |
| MseI | TTAA | 1 cut(s) | 636 |
| MslI | CAYNNNNRTG | 2 cut(s) | 676, 1406 |
| Msp20I | TGGCCA | 1 cut(s) | 847 |
| MspA1I | CMGCKG | 1 cut(s) | 1148 |
| MspI | CCGG | 4 cut(s) | 35, 664, 1192, 1453 |
| MspR9I | CCNGG | 8 cut(s) | 35, 330, 413, 488, 1192, 1422, 1588, 1822 |
| MunI | CAATTG | 3 cut(s) | 233, 870, 1661 |
| Mva1269I | GAATGC | 2 cut(s) | 286, 1909 |
| MvaI | CCWGG | 6 cut(s) | 330, 413, 488, 1422, 1588, 1822 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 52, 1296 |
| NciI | CCSGG | 2 cut(s) | 35, 1192 |
| NcoI | CCATGG | 1 cut(s) | 1030 |
| NdeI | CATATG | 4 cut(s) | 367, 939, 2132, 2191 |
| NsiI | ATGCAT | 1 cut(s) | 28 |
| NspI | RCATGY | 3 cut(s) | 805, 1405, 1517 |
| NspV | TTCGAA | 1 cut(s) | 598 |
| PagI | TCATGA | 1 cut(s) | 471 |
| PciI | ACATGT | 1 cut(s) | 1401 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2115 |
| PctI | GAATGC | 2 cut(s) | 286, 1909 |
| PdmI | GAANNNNTTC | 1 cut(s) | 189 |
| PfeI | GAWTC | 9 cut(s) | 143, 373, 729, 1187, 1547, 1859, 1930, 1970, 2020 |
| PfoI | TCCNGGA | 1 cut(s) | 411 |
| PkrI | GCNGC | 7 cut(s) | 48, 175, 200, 614, 1147, 1242, 1461 |
| PleI | GAGTC | 1 cut(s) | 1678 |
| PpsI | GAGTC | 1 cut(s) | 1678 |
| Ppu21I | YACGTR | 2 cut(s) | 1367, 1408 |
| PpuMI | RGGWCCY | 4 cut(s) | 89, 904, 2152, 2211 |
| PscI | ACATGT | 1 cut(s) | 1401 |
| Psp124BI | GAGCTC | 1 cut(s) | 1957 |
| Psp5II | RGGWCCY | 4 cut(s) | 89, 904, 2152, 2211 |
| Psp6I | CCWGG | 6 cut(s) | 328, 411, 486, 1420, 1586, 1820 |
| PspFI | CCCAGC | 1 cut(s) | 222 |
| PspGI | CCWGG | 6 cut(s) | 328, 411, 486, 1420, 1586, 1820 |
| PspPPI | RGGWCCY | 4 cut(s) | 89, 904, 2152, 2211 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 965, 997 |
| PstI | CTGCAG | 3 cut(s) | 617, 1245, 1555 |
| PstNI | CAGNNNCTG | 1 cut(s) | 452 |
| PsuI | RGATCY | 6 cut(s) | 16, 563, 1568, 1865, 2145, 2204 |
| PvuII | CAGCTG | 1 cut(s) | 1148 |
| RsaI | GTAC | 3 cut(s) | 1000, 1406, 2173 |
| RsaNI | GTAC | 3 cut(s) | 999, 1405, 2172 |
| RseI | CAYNNNNRTG | 2 cut(s) | 676, 1406 |
| SacI | GAGCTC | 1 cut(s) | 1957 |
| SalI | GTCGAC | 1 cut(s) | 2030 |
| SaqAI | TTAA | 1 cut(s) | 636 |
| SatI | GCNGC | 7 cut(s) | 47, 174, 199, 613, 1146, 1241, 1460 |
| ScaI | AGTACT | 1 cut(s) | 1000 |
| SchI | GAGTC | 1 cut(s) | 1679 |
| ScrFI | CCNGG | 8 cut(s) | 35, 330, 413, 488, 1192, 1422, 1588, 1822 |
| SduI | GDGCHC | 5 cut(s) | 660, 1084, 1705, 1957, 2064 |
| SfaNI | GCATC | 7 cut(s) | 35, 72, 265, 445, 1227, 1747, 2015 |
| SfcI | CTRYAG | 3 cut(s) | 613, 1241, 1551 |
| SfuI | TTCGAA | 1 cut(s) | 598 |
| SinI | GGWCC | 7 cut(s) | 89, 904, 925, 2137, 2152, 2196, 2211 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 676, 1406 |
| SsiI | CCGC | 5 cut(s) | 47, 199, 928, 2140, 2199 |
| SspMI | CTAG | 7 cut(s) | 798, 978, 1634, 1682, 1851, 2156, 2215 |
| SstI | GAGCTC | 1 cut(s) | 1957 |
| StyD4I | CCNGG | 8 cut(s) | 33, 328, 411, 486, 1190, 1420, 1586, 1820 |
| StyI | CCWWGG | 3 cut(s) | 733, 1030, 2155 |
| TaaI | ACNGT | 6 cut(s) | 595, 1375, 1797, 1813, 1831, 1885 |
| TaiI | ACGT | 7 cut(s) | 706, 1172, 1278, 1369, 1410, 1440, 1649 |
| TatI | WGTACW | 1 cut(s) | 998 |
| TauI | GCSGC | 2 cut(s) | 49, 201 |
| TfiI | GAWTC | 9 cut(s) | 143, 373, 729, 1187, 1547, 1859, 1930, 1970, 2020 |
| Tru1I | TTAA | 1 cut(s) | 636 |
| Tru9I | TTAA | 1 cut(s) | 636 |
| TscAI | CASTG | 5 cut(s) | 842, 1079, 1250, 1301, 1890 |
| TseI | GCWGC | 5 cut(s) | 173, 612, 1145, 1240, 1459 |
| TspGWI | ACGGA | 1 cut(s) | 272 |
| TspRI | CASTG | 5 cut(s) | 842, 1079, 1250, 1301, 1890 |
| VpaK11BI | GGWCC | 7 cut(s) | 89, 904, 925, 2137, 2152, 2196, 2211 |
| XapI | RAATTY | 2 cut(s) | 476, 630 |
| XceI | RCATGY | 3 cut(s) | 805, 1405, 1517 |
| XmaJI | CCTAGG | 1 cut(s) | 2155 |
| XmiI | GTMKAC | 2 cut(s) | 830, 2031 |
| XmnI | GAANNNNTTC | 1 cut(s) | 189 |
| XspI | CTAG | 7 cut(s) | 798, 978, 1634, 1682, 1851, 2156, 2215 |
| ZraI | GACGTC | 1 cut(s) | 1647 |
| ZrmI | AGTACT | 1 cut(s) | 1000 |
| Zsp2I | ATGCAT | 1 cut(s) | 28 |