RLG00000019895

Poly(A) polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000312
Physical Location & Seq
Reverse (-)
58229254 .. 58234148
4895 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019895

Sequence Viewer

Length: 2100 bp
ATGGTGAGCGCAGACGGATTGAGTGCTTCCCCGCCGCCGCCGCCGCCGCAGGCGGTGTCGGCAAAGCAATTCGGGGTCACAAAGCCGATTTCGACGGCCGGGCCTACTGAAGCCGATACTCAGAGAACTTTAGAGTTGGAGAAGGATGGGCTTGATTTTATCCGGAAAGATTGTGGCATGCTTATTAAATTTCTAGCAGAAGCCATTATTAGTAATATTGTTAAGGATTGGGTAAAGCAACTTACTCAGTTGAGAGGATACACAGATCAAATGGTTGAGGACGCTAATGCTCTTATTTTTACATTTGGTTCCTATAGGCTTGGGGTACATGGCCCTGGGGCTGACATAGACACATTGTGTGTTGGGCCATCTTATGTCAACCGTGAGGAAGACTTCTTCTTTGTATTGCACAACATTCTGGCTGAAATGGAAGAAGTGACTGAACTGCAACCAGTTCCAGATGCCCATGTTCCCGTAATGAAGTTTAAGTTTGATGGAATATCAATCGACCTCCTTTATGCAAGTGTTTCTCTGTTGGTTGTACCAGAAGACCTGGACATTTCTGATGTATCTCTGTTGTACAACATCGATGAGCCTACTGTTCGAAGTCTTAATGGCTGCAGGGTTGCTGATCAAATTCTTAAGCTTGTTCCAAATGTTGAGCATTTCTGTACAACACTCCGATGCTTGAAGTTTTGGGCGAAAAGGCGTGGTGTTTATTCCAATGTTACTGGATTTCTTGGTGGTGTGAACTGGGCTCTCCTTGTTGCCCGAGTGTGTCAACTTTATCCTAATGCAGGTCCTAGCATGCTGGTTTCTCGATTTTTTAGAGTCTACACACAGTGGCGATGGCCAAATCCTGTTATGTTATGTGCTATTGAAGAGGATGAACTTGGTTTTAATGTTTGGGACCCACGCAAAAATCCTCGCGACCGTACTCATCATATGCCAATTATAACTCCTGCATACCCTTGTATGAACTCTAGCTACAATGTTTCTAAAAGTACCCTCCGTGTCATGATGGAGCAGTTTCAGCAGGGAAACAAGATTTGTGAGGAAATAGAGCTGAATAAAGCTAGGTGGTGTGCTCTGTTTGAACCATATTTGTTCTTTGAGAGCTACAAGAACTATCTCCAGGTCGACATCATTGCAGTTGATGCTGATGATCTGCGTGCTTGGAAAGGCTGGGTGGAATCTCGGTTGAGGCAACTGACTCTAATGATTGAGCGGGATACTTTGGAGATGTTACAATGTCACCCATATCCTCATGAATATGTTGATACGACCAAACAGTGTGCACATAGTGCTTTCTTTATGGGTTTGCAGAGGAAACAAGGTGAAAAGGTTCAGGGTCAGCAGTTCGATATACGAGGGACTGTTGATGAGTTCAAGCAGTCCATTGAGATGTACAATTTTTGGAAACCTGGGATGGAAATTTATGTTTCTCACGTTCGTAGAAGGCAACTTCCTTCTTATGTGTTTCCTGATGGTTGTAAACGACCTAGACCATCTAGAGCTGTGGCCCTGCGGCATGATAATGAAGTTAGTCCGACGGGTTCTGGTGAGAGACACCTGAAAAGGAAGAATGATCTTGGTGGGGGTTCGCCACCGAAACGACTATCTATTAGCCCTCAGCCCCAGGATTCAATATCTCCTGAAATTATCACTCACAGATCAGGAAGTGCATCTCCAGAGACTTCTGATAAATGCTTTAGTTTGGAGGTAGTTGATTCAAATAAAAAAACGTTGTCCGGTGAACTGGAGCCTGGAGATGCGACCAATTCCAGTGTTGTTACAAATGTTACAAGCATGAGCAGTAGTGAAGATGCTGGATTTGGATCAATGACTGGTAGCTGTGAGGGGAATACTAAAAGTGTTGAAGGAAGCAGTATTGGGAGTAACACTGCTTCTCTATGTGAGGCAGACTCTGAATCTCTTTCAGTTAACGGAGGTGAAAATGGCAACCTTGTGCAAAGTGGATCACAAGAAGGATTAGAGTTGAATGCTGCATTTGGGATGATACTTAATCCTAGCGAGGGAGTAAGATCGGAAGGTGTGCAGAAAGCAGTGATGAGGTTGGGTTTAACCTCAATGGCCTGA

Protein Analysis

700

Amino Acids

77.74

Weight (kDa)

5.39

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_NTPase PF20750 25 - 217 3.7e-63 Poly(A) polymerase nucleotidyltransferase domain
NTP_transf_2 PF01909 83 - 172 5e-11 Nucleotidyltransferase domain
PAP_central PF04928 222 - 366 2.6e-69 Poly(A) polymerase central domain
PAP_RNA-bind PF04926 370 - 429 9.3e-11 Poly(A) polymerase predicted RNA binding domain
PAP_RNA-bind PF04926 427 - 502 6.1e-08 Poly(A) polymerase predicted RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000312)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850 AT4G32850
fragaria_vesca FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_2g35240 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471 FvH4_6g32471
malus_domestica MD08G1110400.v1.1 MD09G1202000.v1.1 MD09G1202100.v1.1 MD17G1182800.v1.1
prunus_persica Prupe.1G444600_v2.0.a1 Prupe.3G057600_v2.0.a1 Prupe.3G057600_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1 Prupe.3G057700_v2.0.a1
pyrus_communis pycom08g09190 pycom09g11890 pycom15g08420 pycom17g19200
rosa_chinensis RchiOBHm_Chr2g0140721 RchiOBHm_Chr2g0140741 RchiOBHm_Chr2g0140761 RchiOBHm_Chr6g0306691
rosa_laevigata RLG00000010773 RLG00000019895 RLG00000019896
rosa_multiflora Rmu_co8193342.1_g000001 Rmu_sc0003342.1_g000015 Rmu_sc0003342.1_g000021 Rmu_sc0003342.1_g000026 Rmu_sc0005201.1_g000002 Rmu_sc0017785.1_g000009
rosa_roxburghii Rroxscaffold_2G00104510 Rroxscaffold_2G00104540 Rroxscaffold_7G00161540
rosa_rugosa Rorug02G0364600 Rorug02G0364700 Rorug02G0364800 Rorug02G0364900 Rorug02G0365000 Rorug06G0351600 Rorug06G0351700
rosa_samantha Rh2AG416100 Rh2AG416200 Rh2AG416300 Rh2BG425700 Rh2BG425800 Rh2BG425900 Rh2CG402300 Rh2CG402400 Rh2CG402500 Rh2DG435600 Rh2DG435700 Rh2DG435800 Rh6AG464000 Rh6BG437000 Rh6CG478100 Rh6DG464900
rosa_wichuraiana Rw2G034050 Rw2G034060 Rw6G040420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 956
Acc36I ACCTGC 1 cut(s) 788
AccBSI CCGCTC 1 cut(s) 1228
AccI GTMKAC 2 cut(s) 834, 1140
AccII CGCG 1 cut(s) 930
AccIII TCCGGA 1 cut(s) 162
AciI CCGC 9 cut(s) 32, 35, 38, 41, 44, 47, 53, 1228, 1528
AclI AACGTT 1 cut(s) 1745
AclWI GGATC 2 cut(s) 1846, 1987
AcoI YGGCCR 2 cut(s) 96, 851
AcsI RAATTY 3 cut(s) 188, 636, 1434
AcuI CTGAAG 1 cut(s) 129
AdeI CACNNNGTG 3 cut(s) 357, 843, 1304
AfaI GTAC 7 cut(s) 327, 543, 581, 673, 937, 1006, 1409
AfiI CCNNNNNNNGG 2 cut(s) 797, 2036
AflII CTTAAG 1 cut(s) 641
AgsI TTSAA 8 cut(s) 691, 881, 1097, 1390, 1647, 1734, 1880, 2002
AjnI CCWGG 6 cut(s) 334, 552, 1134, 1423, 1638, 1765
AluBI AGCT 7 cut(s) 646, 987, 1066, 1076, 1119, 1517, 1854
AluI AGCT 7 cut(s) 646, 987, 1066, 1076, 1119, 1517, 1854
Alw21I GWGCWC 2 cut(s) 1090, 1300
Alw26I GTCTC 2 cut(s) 1561, 1688
Alw44I GTGCAC 1 cut(s) 1296
AlwI GGATC 2 cut(s) 1846, 1987
AlwNI CAGNNNCTG 1 cut(s) 1928
Ama87I CYCGRG 1 cut(s) 771
Aor13HI TCCGGA 1 cut(s) 162
AoxI GGCC 7 cut(s) 96, 101, 331, 365, 851, 1521, 2094
ApaLI GTGCAC 1 cut(s) 1296
ApeKI GCWGC 2 cut(s) 618, 2006
ApoI RAATTY 3 cut(s) 188, 636, 1434
ArsI GACNNNNNNTTYG 2 cut(s) 383, 415
Asp700I GAANNNNTTC 1 cut(s) 1344
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 6 cut(s) 101, 332, 365, 800, 910, 1522
AsuC2I CCSGG 1 cut(s) 100
AsuHPI GGTGA 6 cut(s) 16, 1247, 1349, 1574, 1766, 1964
AsuII TTCGAA 1 cut(s) 604
AvaI CYCGRG 1 cut(s) 771
AvaII GGWCC 2 cut(s) 800, 910
BaeGI GKGCMC 1 cut(s) 1300
BalI TGGCCA 1 cut(s) 853
BanII GRGCYC 1 cut(s) 760
BarI GAAGNNNNNNTAC 2 cut(s) 1891, 1923
BbsI GAAGAC 2 cut(s) 396, 555
Bbv12I GWGCWC 2 cut(s) 1090, 1300
BbvCI CCTCAGC 1 cut(s) 1632
BbvI GCAGC 2 cut(s) 605, 1993
BccI CCATC 8 cut(s) 140, 376, 488, 843, 1015, 1423, 1481, 1516
BceAI ACGGC 1 cut(s) 111
BcgI CGANNNNNNTGC 2 cut(s) 1130, 1164
BciT130I CCWGG 6 cut(s) 336, 554, 1136, 1425, 1640, 1767
BciVI GTATCC 2 cut(s) 251, 1225
BclI TGATCA 1 cut(s) 631
BcnI CCSGG 1 cut(s) 100
BcoDI GTCTC 2 cut(s) 1561, 1688
BfaI CTAG 7 cut(s) 194, 804, 984, 1077, 1503, 1512, 2031
BfmI CTRYAG 2 cut(s) 313, 619
BfrI CTTAAG 1 cut(s) 641
BfuAI ACCTGC 1 cut(s) 788
BfuI GTATCC 2 cut(s) 251, 1225
BisI GCNGC 8 cut(s) 35, 38, 41, 44, 47, 619, 1529, 2007
BlsI GCNGC 8 cut(s) 36, 39, 42, 45, 48, 620, 1530, 2008
Bme1390I CCNGG 7 cut(s) 100, 336, 554, 1136, 1425, 1640, 1767
Bme18I GGWCC 2 cut(s) 800, 910
BmeT110I CYCGRG 1 cut(s) 771
BmgT120I GGNCC 6 cut(s) 101, 332, 365, 800, 910, 1522
BmiI GGNNCC 4 cut(s) 310, 911, 912, 1764
BmrFI CCNGG 7 cut(s) 100, 336, 554, 1136, 1425, 1640, 1767
BmrI ACTGGG 1 cut(s) 763
BmsI GCATC 6 cut(s) 451, 674, 1147, 1694, 1762, 1816
BmuI ACTGGG 1 cut(s) 763
BpiI GAAGAC 2 cut(s) 396, 555
BplI GAGNNNNNCTC 2 cut(s) 1910, 1942
BpmI CTGGAG 4 cut(s) 1118, 1674, 1781, 1788
Bpu10I CCTNAGC 1 cut(s) 1632
Bpu14I TTCGAA 1 cut(s) 604
BpuMI CCSGG 1 cut(s) 100
Bsa29I ATCGAT 1 cut(s) 588
BsaBI GATNNNNATC 1 cut(s) 1837
BsaJI CCNNGG 4 cut(s) 334, 335, 1424, 1638
BsaWI WCCGGW 2 cut(s) 162, 1751
Bsc4I CCNNNNNNNGG 2 cut(s) 797, 2036
Bse1I ACTGG 6 cut(s) 452, 736, 758, 1764, 1785, 1852
Bse3DI GCAATG 1 cut(s) 1146
Bse8I GATNNNNATC 1 cut(s) 1837
BseAI TCCGGA 1 cut(s) 162
BseBI CCWGG 6 cut(s) 336, 554, 1136, 1425, 1640, 1767
BseCI ATCGAT 1 cut(s) 588
BseDI CCNNGG 4 cut(s) 334, 335, 1424, 1638
BseGI GGATG 4 cut(s) 151, 892, 1434, 2022
BseJI GATNNNNATC 1 cut(s) 1837
BseLI CCNNNNNNNGG 2 cut(s) 797, 2036
BseMI GCAATG 1 cut(s) 1146
BseMII CTCAG 3 cut(s) 134, 260, 1646
BseNI ACTGG 6 cut(s) 452, 736, 758, 1764, 1785, 1852
BseSI GKGCMC 1 cut(s) 1300
BseX3I CGGCCG 1 cut(s) 96
BseXI GCAGC 2 cut(s) 605, 1993
BseYI CCCAGC 1 cut(s) 1185
BsgI GTGCAG 1 cut(s) 2078
Bsh1236I CGCG 1 cut(s) 930
Bsh1285I CGRYCG 2 cut(s) 99, 934
BshFI GGCC 7 cut(s) 98, 103, 333, 367, 853, 1523, 2096
BshVI ATCGAT 1 cut(s) 588
BsiEI CGRYCG 2 cut(s) 99, 934
BsiHKAI GWGCWC 2 cut(s) 1090, 1300
BsiHKCI CYCGRG 1 cut(s) 771
BsiSI CCGG 3 cut(s) 99, 163, 1752
BslFI GGGAC 2 cut(s) 923, 1387
BslI CCNNNNNNNGG 2 cut(s) 797, 2036
BsmAI GTCTC 2 cut(s) 1561, 1688
BsmFI GGGAC 2 cut(s) 923, 1387
BsmI GAATGC 1 cut(s) 2008
BsnI GGCC 7 cut(s) 98, 103, 333, 367, 853, 1523, 2096
BsoBI CYCGRG 1 cut(s) 771
Bsp119I TTCGAA 1 cut(s) 604
Bsp1286I GDGCHC 3 cut(s) 760, 1090, 1300
Bsp13I TCCGGA 1 cut(s) 162
Bsp1407I TGTACA 3 cut(s) 579, 671, 1407
Bsp143I GATC 8 cut(s) 265, 631, 1165, 1588, 1673, 1838, 1979, 2045
Bsp68I TCGCGA 1 cut(s) 930
BspACI CCGC 9 cut(s) 32, 35, 38, 41, 44, 47, 53, 1228, 1528
BspANI GGCC 7 cut(s) 98, 103, 333, 367, 853, 1523, 2096
BspCNI CTCAG 3 cut(s) 133, 259, 1645
BspDI ATCGAT 1 cut(s) 588
BspEI TCCGGA 1 cut(s) 162
BspFNI CGCG 1 cut(s) 930
BspHI TCATGA 2 cut(s) 1017, 1267
BspLI GGNNCC 4 cut(s) 310, 911, 912, 1764
BspMAI CTGCAG 1 cut(s) 623
BspMI ACCTGC 1 cut(s) 788
BspPI GGATC 2 cut(s) 1846, 1987
BspT104I TTCGAA 1 cut(s) 604
BspTI CTTAAG 1 cut(s) 641
BsrBI CCGCTC 1 cut(s) 1228
BsrDI GCAATG 1 cut(s) 1146
BsrGI TGTACA 3 cut(s) 579, 671, 1407
BsrI ACTGG 6 cut(s) 452, 736, 758, 1764, 1785, 1852
BssECI CCNNGG 4 cut(s) 334, 335, 1424, 1638
BssMI GATC 8 cut(s) 265, 631, 1165, 1588, 1673, 1838, 1979, 2045
Bst2UI CCWGG 6 cut(s) 336, 554, 1136, 1425, 1640, 1767
Bst4CI ACNGT 6 cut(s) 383, 601, 843, 935, 1293, 1378
Bst6I CTCTTC 1 cut(s) 876
BstAFI CTTAAG 1 cut(s) 641
BstAPI GCANNNNNTGC 2 cut(s) 1157, 1304
BstAUI TGTACA 3 cut(s) 579, 671, 1407
BstBI TTCGAA 1 cut(s) 604
BstC8I GCNNGC 4 cut(s) 51, 179, 809, 1173
BstDEI CTNAG 3 cut(s) 120, 246, 1632
BstENI CCTNNNNNAGG 1 cut(s) 795
BstF5I GGATG 4 cut(s) 151, 892, 1434, 2022
BstFNI CGCG 1 cut(s) 930
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 8 cut(s) 268, 634, 1168, 1591, 1676, 1841, 1982, 2048
BstMAI GTCTC 2 cut(s) 1561, 1688
BstMBI GATC 8 cut(s) 265, 631, 1165, 1588, 1673, 1838, 1979, 2045
BstMCI CGRYCG 2 cut(s) 99, 934
BstMWI GCNNNNNNNGC 7 cut(s) 40, 43, 46, 59, 1033, 1157, 1304
BstNI CCWGG 6 cut(s) 336, 554, 1136, 1425, 1640, 1767
BstNSI RCATGY 2 cut(s) 181, 811
BstSCI CCNGG 7 cut(s) 98, 334, 552, 1134, 1423, 1638, 1765
BstSFI CTRYAG 2 cut(s) 313, 619
BstSLI GKGCMC 1 cut(s) 1300
BstUI CGCG 1 cut(s) 930
BstV1I GCAGC 2 cut(s) 605, 1993
BstV2I GAAGAC 2 cut(s) 396, 555
BstZI CGGCCG 1 cut(s) 96
Bsu15I ATCGAT 1 cut(s) 588
BsuI GTATCC 2 cut(s) 251, 1225
BsuRI GGCC 7 cut(s) 98, 103, 333, 367, 853, 1523, 2096
BsuTUI ATCGAT 1 cut(s) 588
BtgZI GCGATG 1 cut(s) 862
BtsCI GGATG 4 cut(s) 151, 892, 1434, 2022
BtsI GCAGTG 2 cut(s) 1902, 2073
BtsIMutI CAGTG 5 cut(s) 848, 1298, 1792, 1902, 2073
BtuMI TCGCGA 1 cut(s) 930
BveI ACCTGC 1 cut(s) 788
Cac8I GCNNGC 4 cut(s) 51, 179, 809, 1173
CaiI CAGNNNCTG 1 cut(s) 1928
CciI TCATGA 2 cut(s) 1017, 1267
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 6 cut(s) 101, 332, 365, 800, 910, 1522
ClaI ATCGAT 1 cut(s) 588
CseI GACGC 1 cut(s) 290
Csp6I GTAC 7 cut(s) 326, 542, 580, 672, 936, 1005, 1408
CviAII CATG 8 cut(s) 178, 329, 467, 808, 1018, 1268, 1532, 1810
CviQI GTAC 7 cut(s) 326, 542, 580, 672, 936, 1005, 1408
DdeI CTNAG 3 cut(s) 120, 246, 1632
DpnI GATC 8 cut(s) 267, 633, 1167, 1590, 1675, 1840, 1981, 2047
DpnII GATC 8 cut(s) 265, 631, 1165, 1588, 1673, 1838, 1979, 2045
DraIII CACNNNGTG 3 cut(s) 357, 843, 1304
EaeI YGGCCR 2 cut(s) 96, 851
EagI CGGCCG 1 cut(s) 96
Eam1104I CTCTTC 1 cut(s) 876
EarI CTCTTC 1 cut(s) 876
EclXI CGGCCG 1 cut(s) 96
Eco24I GRGCYC 1 cut(s) 760
Eco47I GGWCC 2 cut(s) 800, 910
Eco52I CGGCCG 1 cut(s) 96
Eco57I CTGAAG 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 771
EcoNI CCTNNNNNAGG 1 cut(s) 795
EcoO109I RGGNCCY 2 cut(s) 800, 910
EcoRII CCWGG 6 cut(s) 334, 552, 1134, 1423, 1638, 1765
EcoT38I GRGCYC 1 cut(s) 760
FaeI CATG 8 cut(s) 181, 332, 470, 811, 1021, 1271, 1535, 1813
FaqI GGGAC 2 cut(s) 923, 1387
FatI CATG 8 cut(s) 177, 328, 466, 807, 1017, 1267, 1531, 1809
FauI CCCGC 2 cut(s) 39, 1221
FauNDI CATATG 1 cut(s) 945
FbaI TGATCA 1 cut(s) 631
FblI GTMKAC 2 cut(s) 834, 1140
Fnu4HI GCNGC 8 cut(s) 35, 38, 41, 44, 47, 619, 1529, 2007
FokI GGATG 4 cut(s) 158, 899, 1441, 2029
FriOI GRGCYC 1 cut(s) 760
Fsp4HI GCNGC 8 cut(s) 35, 38, 41, 44, 47, 619, 1529, 2007
FspBI CTAG 7 cut(s) 194, 804, 984, 1077, 1503, 1512, 2031
GlaI GCGC 1 cut(s) 10
GluI GCNGC 8 cut(s) 35, 38, 41, 44, 47, 619, 1529, 2007
GsaI CCCAGC 1 cut(s) 1189
GsuI CTGGAG 4 cut(s) 1118, 1674, 1781, 1788
HaeIII GGCC 7 cut(s) 98, 103, 333, 367, 853, 1523, 2096
HapII CCGG 3 cut(s) 99, 163, 1752
HgaI GACGC 1 cut(s) 290
HhaI GCGC 1 cut(s) 11
Hin1II CATG 8 cut(s) 181, 332, 470, 811, 1021, 1271, 1535, 1813
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HincII GTYRAC 4 cut(s) 379, 782, 1141, 1945
HindII GTYRAC 4 cut(s) 379, 782, 1141, 1945
HindIII AAGCTT 1 cut(s) 644
HinfI GANTC 7 cut(s) 831, 1193, 1213, 1643, 1730, 1925, 1931
HpaI GTTAAC 1 cut(s) 1945
HpaII CCGG 3 cut(s) 99, 163, 1752
HphI GGTGA 6 cut(s) 16, 1247, 1349, 1574, 1766, 1964
Hpy166II GTNNAC 9 cut(s) 379, 751, 782, 835, 1141, 1298, 1496, 1757, 1945
Hpy188I TCNGA 7 cut(s) 123, 565, 683, 1551, 1702, 1930, 2050
Hpy8I GTNNAC 9 cut(s) 379, 751, 782, 835, 1141, 1298, 1496, 1757, 1945
Hpy99I CGWCG 2 cut(s) 97, 1555
HpyAV CCTTC 6 cut(s) 136, 1452, 1479, 1874, 1982, 2045
HpyCH4III ACNGT 6 cut(s) 383, 601, 843, 935, 1293, 1378
HpyCH4IV ACGT 2 cut(s) 1449, 1745
HpyF10VI GCNNNNNNNGC 7 cut(s) 40, 43, 46, 59, 1033, 1157, 1304
HpyF3I CTNAG 3 cut(s) 120, 246, 1632
HpySE526I ACGT 2 cut(s) 1449, 1745
Hsp92II CATG 8 cut(s) 181, 332, 470, 811, 1021, 1271, 1535, 1813
HspAI GCGC 1 cut(s) 9
KflI GGGWCCC 1 cut(s) 910
Kpn2I TCCGGA 1 cut(s) 162
Ksp22I TGATCA 1 cut(s) 631
KspAI GTTAAC 1 cut(s) 1945
Kzo9I GATC 8 cut(s) 265, 631, 1165, 1588, 1673, 1838, 1979, 2045
LmnI GCTCC 2 cut(s) 1024, 1762
Lsp1109I GCAGC 2 cut(s) 605, 1993
LweI GCATC 6 cut(s) 451, 674, 1147, 1694, 1762, 1816
MaeI CTAG 7 cut(s) 194, 804, 984, 1077, 1503, 1512, 2031
MaeII ACGT 2 cut(s) 1449, 1745
MaeIII GTNAC 8 cut(s) 76, 436, 727, 1245, 1253, 1792, 1801, 1898
MalI GATC 8 cut(s) 267, 633, 1167, 1590, 1675, 1840, 1981, 2047
MbiI CCGCTC 1 cut(s) 1228
MboI GATC 8 cut(s) 265, 631, 1165, 1588, 1673, 1838, 1979, 2045
MboII GAAGA 7 cut(s) 388, 401, 443, 560, 893, 1594, 1835
MhlI GDGCHC 3 cut(s) 760, 1090, 1300
MlsI TGGCCA 1 cut(s) 853
MluCI AATT 8 cut(s) 68, 188, 636, 951, 1411, 1434, 1659, 1780
MluNI TGGCCA 1 cut(s) 853
MlyI GAGTC 3 cut(s) 840, 1207, 1919
MmeI TCCRAC 2 cut(s) 117, 1574
Mox20I TGGCCA 1 cut(s) 853
MroI TCCGGA 1 cut(s) 162
MroXI GAANNNNTTC 1 cut(s) 1344
MscI TGGCCA 1 cut(s) 853
MseI TTAA 9 cut(s) 186, 222, 486, 612, 642, 900, 1944, 2025, 2084
MslI CAYNNNNRTG 4 cut(s) 682, 1272, 1403, 1536
Msp20I TGGCCA 1 cut(s) 853
MspCI CTTAAG 1 cut(s) 641
MspI CCGG 3 cut(s) 99, 163, 1752
MspR9I CCNGG 7 cut(s) 100, 336, 554, 1136, 1425, 1640, 1767
Mva1269I GAATGC 1 cut(s) 2008
MvaI CCWGG 6 cut(s) 336, 554, 1136, 1425, 1640, 1767
MvnI CGCG 1 cut(s) 930
MwoI GCNNNNNNNGC 7 cut(s) 40, 43, 46, 59, 1033, 1157, 1304
NciI CCSGG 1 cut(s) 100
NdeI CATATG 1 cut(s) 945
NdeII GATC 8 cut(s) 265, 631, 1165, 1588, 1673, 1838, 1979, 2045
NlaIII CATG 8 cut(s) 181, 332, 470, 811, 1021, 1271, 1535, 1813
NlaIV GGNNCC 4 cut(s) 310, 911, 912, 1764
NmuCI GTSAC 3 cut(s) 76, 436, 1253
NruI TCGCGA 1 cut(s) 930
NspI RCATGY 2 cut(s) 181, 811
NspV TTCGAA 1 cut(s) 604
PaeI GCATGC 2 cut(s) 181, 811
PagI TCATGA 2 cut(s) 1017, 1267
PasI CCCWGGG 1 cut(s) 335
PctI GAATGC 1 cut(s) 2008
PdmI GAANNNNTTC 1 cut(s) 1344
PfeI GAWTC 4 cut(s) 1193, 1643, 1730, 1931
PkrI GCNGC 8 cut(s) 36, 39, 42, 45, 48, 620, 1530, 2008
PleI GAGTC 3 cut(s) 839, 1207, 1919
PpsI GAGTC 3 cut(s) 839, 1207, 1919
PpuMI RGGWCCY 2 cut(s) 800, 910
PsiI TTATAA 1 cut(s) 956
Psp1406I AACGTT 1 cut(s) 1745
Psp5II RGGWCCY 2 cut(s) 800, 910
Psp6I CCWGG 6 cut(s) 334, 552, 1134, 1423, 1638, 1765
PspFI CCCAGC 1 cut(s) 1185
PspGI CCWGG 6 cut(s) 334, 552, 1134, 1423, 1638, 1765
PspN4I GGNNCC 4 cut(s) 310, 911, 912, 1764
PspPI GGNCC 6 cut(s) 101, 332, 365, 800, 910, 1522
PspPPI RGGWCCY 2 cut(s) 800, 910
PsrI GAACNNNNNNTAC 2 cut(s) 971, 1003
PstI CTGCAG 1 cut(s) 623
PstNI CAGNNNCTG 1 cut(s) 1928
RruI TCGCGA 1 cut(s) 930
RsaI GTAC 7 cut(s) 327, 543, 581, 673, 937, 1006, 1409
RsaNI GTAC 7 cut(s) 326, 542, 580, 672, 936, 1005, 1408
RseI CAYNNNNRTG 4 cut(s) 682, 1272, 1403, 1536
SalI GTCGAC 1 cut(s) 1139
SaqAI TTAA 9 cut(s) 186, 222, 486, 612, 642, 900, 1944, 2025, 2084
SatI GCNGC 8 cut(s) 35, 38, 41, 44, 47, 619, 1529, 2007
Sau3AI GATC 8 cut(s) 265, 631, 1165, 1588, 1673, 1838, 1979, 2045
Sau96I GGNCC 6 cut(s) 101, 332, 365, 800, 910, 1522
SchI GAGTC 3 cut(s) 840, 1207, 1919
ScrFI CCNGG 7 cut(s) 100, 336, 554, 1136, 1425, 1640, 1767
SduI GDGCHC 3 cut(s) 760, 1090, 1300
SfaNI GCATC 6 cut(s) 451, 674, 1147, 1694, 1762, 1816
SfcI CTRYAG 2 cut(s) 313, 619
SfuI TTCGAA 1 cut(s) 604
SinI GGWCC 2 cut(s) 800, 910
SmiMI CAYNNNNRTG 4 cut(s) 682, 1272, 1403, 1536
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
SphI GCATGC 2 cut(s) 181, 811
Sse9I AATT 8 cut(s) 68, 188, 636, 951, 1411, 1434, 1659, 1780
SsiI CCGC 9 cut(s) 32, 35, 38, 41, 44, 47, 53, 1228, 1528
SspI AATATT 1 cut(s) 217
SspMI CTAG 7 cut(s) 194, 804, 984, 1077, 1503, 1512, 2031
StyD4I CCNGG 7 cut(s) 98, 334, 552, 1134, 1423, 1638, 1765
TaaI ACNGT 6 cut(s) 383, 601, 843, 935, 1293, 1378
TaiI ACGT 2 cut(s) 1452, 1748
TaqI TCGA 7 cut(s) 92, 507, 588, 604, 820, 1140, 1362
TasI AATT 8 cut(s) 68, 188, 636, 951, 1411, 1434, 1659, 1780
TatI WGTACW 3 cut(s) 579, 671, 1407
TauI GCSGC 6 cut(s) 37, 40, 43, 46, 49, 1531
TfiI GAWTC 4 cut(s) 1193, 1643, 1730, 1931
Tru1I TTAA 9 cut(s) 186, 222, 486, 612, 642, 900, 1944, 2025, 2084
Tru9I TTAA 9 cut(s) 186, 222, 486, 612, 642, 900, 1944, 2025, 2084
TscAI CASTG 5 cut(s) 848, 1298, 1792, 1909, 2073
TseFI GTSAC 3 cut(s) 76, 436, 1253
TseI GCWGC 2 cut(s) 618, 2006
Tsp45I GTSAC 3 cut(s) 76, 436, 1253
TspDTI ATGAA 5 cut(s) 494, 903, 992, 1284, 1554
TspGWI ACGGA 3 cut(s) 30, 1001, 1962
TspRI CASTG 5 cut(s) 848, 1298, 1792, 1909, 2073
Vha464I CTTAAG 1 cut(s) 641
VneI GTGCAC 1 cut(s) 1296
VpaK11BI GGWCC 2 cut(s) 800, 910
XagI CCTNNNNNAGG 1 cut(s) 795
XapI RAATTY 3 cut(s) 188, 636, 1434
XbaI TCTAGA 1 cut(s) 1511
XceI RCATGY 2 cut(s) 181, 811
XmiI GTMKAC 2 cut(s) 834, 1140
XmnI GAANNNNTTC 1 cut(s) 1344
XspI CTAG 7 cut(s) 194, 804, 984, 1077, 1503, 1512, 2031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.