FvH4_6g52470

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001421
Physical Location & Seq
Forward (+)
38628064 .. 38630216
2153 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g52470.t2

Sequence Viewer

Length: 1191 bp
ATGGTGAGAGAAGAAGAACCAAAACAGAGCCCAAGAACTCAACCCAGAGAATCAAACCCAATTGAGATCGGAAACAGCACAAGTGGCGGGACTAGCTCGTTGTTCAGCCCCAGATTCAAATCGGCGGCCGCCATAGCCGGTTGGGACGAAGAGAGTCTCTTGATGGCGAGCCTCATTGTCGACGACACGCCGGAGCAAGATACCAAGCAGAAGAAACGATCTGTTTTCAGATTTTTTGGGTGGTTCAGAAAGCGGAGAGTTCAGCGAAAGAGCCCGGTTTCGATCCCAGTGGGTGTTCTTGACCTTGATAGTGTAGAAACTACAAAAGAGGAGAGTGTGAAGGAGAAGGTAGAGCCTGAGATTGTTGTTGTGAATGAAGAAAAGAAAAGAAGGGAAAATGAGAAGAAGGGTGAGAGCTCTGGTGCTTCGTGCTCGAGCTCGAATCTTCCTTGCATAGATAAGCTCAGGGATGAGCTTTCTTGTGCTATCTGCTTGGAGATTTGCTTTGAACCCAGTACCACTCCTTGTGGACACAGTTTCTGTATGAAGTGCTTGCGATCTGCTGCGGATAAATGTGGGAAGAGGTGCCCCAAATGCAGGCAGCTAATGAGCAATGGAAGATCTTGCACCGTGAACACGGTTCTGTGGAACACAATTCAGCTTCTGTTTCCGCAAGAAGTTGAAACAAGGAAAGCAGCTGGAGCCTTGAATAGTCGTGAAGAAGCTGAGAGGAAAATCCCTGATAAGGCCTTTAGTAGTAGCAACTCAAGGAATCAAAGCAGAAGAGTATCAGGTAGAGATCTGGCTGCAAGGAGAAGAAGGGCAGTTTTAAGCCAAGATGAAGACGAGGATGATCCTGCGCCACTTGCTATAAGGTTACAGATGAGAGAAGCTCAGAATCAAAATCGGGATCCAGGAACCTTCACTCGACCAGCAAGGATATCAGCAGCTAGAGAAATTCAGCCTATCAGGAGAGTATCAAGCAGAGATGCAAATCCAACGAGTAATAGAAGTGTGCCAAACCAAGATGAGGATGCTGCATTAGCTCTGAGGTTACAGAGGGAGGAGTTTATGGAGGCTTTTAGGGGAACTACTGATCAGTCTAGTGCCTCTCTTTCGTCTGCTAGAGCAAACATCCGAGCCATGGCATCTAGAGCCACTAATCGTGGTGCTGCAACCCGATTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-C3HC4_2

Protein Analysis

397

Amino Acids

44.26

Weight (kDa)

9.32

Isoelectric Point (pI)

71.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4 PF00097 167 - 205 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 167 - 203 3.2e-06 RING-type zinc-finger
zf-C3HC4_2 PF13923 167 - 205 4.2e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 585
AccI GTMKAC 1 cut(s) 180
AciI CCGC 6 cut(s) 87, 125, 129, 253, 566, 671
AclWI GGATC 4 cut(s) 277, 848, 905, 918
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 1 cut(s) 957
AfaI GTAC 1 cut(s) 517
AfiI CCNNNNNNNGG 4 cut(s) 597, 745, 1030, 1144
AgsI TTSAA 4 cut(s) 118, 509, 683, 709
AjnI CCWGG 1 cut(s) 913
Alw21I GWGCWC 3 cut(s) 419, 434, 440
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 4 cut(s) 277, 848, 905, 918
AlwNI CAGNNNCTG 2 cut(s) 540, 664
Ama87I CYCGRG 1 cut(s) 433
AoxI GGCC 2 cut(s) 126, 747
ApeKI GCWGC 7 cut(s) 563, 601, 695, 806, 947, 1037, 1172
ApoI RAATTY 1 cut(s) 957
AspLEI GCGC 1 cut(s) 862
AsuC2I CCSGG 1 cut(s) 275
AsuHPI GGTGA 2 cut(s) 16, 422
AvaI CYCGRG 1 cut(s) 433
BaeGI GKGCMC 1 cut(s) 590
BamHI GGATCC 1 cut(s) 910
BanI GGYRCC 1 cut(s) 585
BanII GRGCYC 4 cut(s) 32, 275, 419, 440
BbsI GAAGAC 1 cut(s) 849
Bbv12I GWGCWC 3 cut(s) 419, 434, 440
BbvI GCAGC 7 cut(s) 550, 613, 707, 793, 959, 1024, 1159
BccI CCATC 1 cut(s) 157
BciT130I CCWGG 1 cut(s) 915
BclI TGATCA 1 cut(s) 1096
BcnI CCSGG 1 cut(s) 275
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 5 cut(s) 93, 951, 1104, 1125, 1152
BglII AGATCT 2 cut(s) 620, 799
BisI GCNGC 9 cut(s) 126, 129, 564, 602, 696, 807, 948, 1038, 1173
BlsI GCNGC 9 cut(s) 127, 130, 565, 603, 697, 808, 949, 1039, 1174
Bme1390I CCNGG 2 cut(s) 275, 915
BmeT110I CYCGRG 1 cut(s) 433
BmiI GGNNCC 4 cut(s) 587, 703, 912, 919
BmrFI CCNGG 2 cut(s) 275, 915
BmrI ACTGGG 2 cut(s) 281, 507
BmsI GCATC 3 cut(s) 979, 1024, 1157
BmuI ACTGGG 2 cut(s) 281, 507
BpiI GAAGAC 1 cut(s) 849
BplI GAGNNNNNCTC 2 cut(s) 877, 909
BpmI CTGGAG 1 cut(s) 720
Bpu10I CCTNAGC 1 cut(s) 464
BpuEI CTTGAG 1 cut(s) 751
BpuMI CCSGG 1 cut(s) 275
BsaBI GATNNNNATC 2 cut(s) 118, 993
BsaJI CCNNGG 1 cut(s) 1143
Bsc4I CCNNNNNNNGG 4 cut(s) 597, 745, 1030, 1144
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 2 cut(s) 287, 513
Bse3DI GCAATG 1 cut(s) 619
Bse8I GATNNNNATC 2 cut(s) 118, 993
BseBI CCWGG 1 cut(s) 915
BseDI CCNNGG 1 cut(s) 1143
BseGI GGATG 4 cut(s) 475, 856, 1039, 1134
BseJI GATNNNNATC 2 cut(s) 118, 993
BseLI CCNNNNNNNGG 4 cut(s) 597, 745, 1030, 1144
BseMI GCAATG 1 cut(s) 619
BseMII CTCAG 5 cut(s) 348, 478, 717, 908, 1040
BseNI ACTGG 2 cut(s) 287, 513
BseRI GAGGAG 2 cut(s) 344, 1079
BseSI GKGCMC 1 cut(s) 590
BseX3I CGGCCG 1 cut(s) 126
BseXI GCAGC 7 cut(s) 550, 613, 707, 793, 959, 1024, 1159
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 2 cut(s) 128, 749
BshNI GGYRCC 1 cut(s) 585
BsiEI CGRYCG 1 cut(s) 129
BsiHKAI GWGCWC 3 cut(s) 419, 434, 440
BsiHKCI CYCGRG 1 cut(s) 433
BsiSI CCGG 3 cut(s) 138, 191, 275
BslFI GGGAC 2 cut(s) 103, 158
BslI CCNNNNNNNGG 4 cut(s) 597, 745, 1030, 1144
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 2 cut(s) 103, 158
BsnI GGCC 2 cut(s) 128, 749
BsoBI CYCGRG 1 cut(s) 433
Bsp1286I GDGCHC 6 cut(s) 32, 275, 419, 434, 440, 590
Bsp143I GATC 9 cut(s) 66, 218, 282, 557, 620, 799, 853, 910, 1096
Bsp19I CCATGG 1 cut(s) 1143
BspACI CCGC 6 cut(s) 87, 125, 129, 253, 566, 671
BspANI GGCC 2 cut(s) 128, 749
BspCNI CTCAG 5 cut(s) 349, 477, 718, 907, 1041
BspLI GGNNCC 4 cut(s) 587, 703, 912, 919
BspPI GGATC 4 cut(s) 277, 848, 905, 918
BspT107I GGYRCC 1 cut(s) 585
BsrDI GCAATG 1 cut(s) 619
BsrFI RCCGGY 1 cut(s) 137
BsrI ACTGG 2 cut(s) 287, 513
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 1 cut(s) 1143
BssMI GATC 9 cut(s) 66, 218, 282, 557, 620, 799, 853, 910, 1096
BssT1I CCWWGG 1 cut(s) 1143
Bst2UI CCWGG 1 cut(s) 915
Bst4CI ACNGT 3 cut(s) 536, 631, 640
Bst6I CTCTTC 3 cut(s) 144, 575, 778
BstC8I GCNNGC 3 cut(s) 169, 554, 599
BstDEI CTNAG 5 cut(s) 357, 464, 726, 894, 1049
BstDSI CCRYGG 1 cut(s) 1143
BstF5I GGATG 4 cut(s) 475, 856, 1039, 1134
BstHHI GCGC 1 cut(s) 862
BstKTI GATC 9 cut(s) 69, 221, 285, 560, 623, 802, 856, 913, 1099
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 9 cut(s) 66, 218, 282, 557, 620, 799, 853, 910, 1096
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 8 cut(s) 84, 93, 134, 594, 701, 866, 1043, 1154
BstNI CCWGG 1 cut(s) 915
BstSCI CCNGG 2 cut(s) 273, 913
BstSLI GKGCMC 1 cut(s) 590
BstV1I GCAGC 7 cut(s) 550, 613, 707, 793, 959, 1024, 1159
BstV2I GAAGAC 1 cut(s) 849
BstX2I RGATCY 3 cut(s) 620, 799, 910
BstYI RGATCY 3 cut(s) 620, 799, 910
BstZI CGGCCG 1 cut(s) 126
BsuRI GGCC 2 cut(s) 128, 749
BtgI CCRYGG 1 cut(s) 1143
BtsCI GGATG 4 cut(s) 475, 856, 1039, 1134
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 3 cut(s) 169, 554, 599
CaiI CAGNNNCTG 2 cut(s) 540, 664
CciNI GCGGCCGC 1 cut(s) 126
CfoI GCGC 1 cut(s) 862
Cfr10I RCCGGY 1 cut(s) 137
Csp6I GTAC 1 cut(s) 516
CviAII CATG 1 cut(s) 1144
CviQI GTAC 1 cut(s) 516
DdeI CTNAG 5 cut(s) 357, 464, 726, 894, 1049
DpnI GATC 9 cut(s) 68, 220, 284, 559, 622, 801, 855, 912, 1098
DpnII GATC 9 cut(s) 66, 218, 282, 557, 620, 799, 853, 910, 1096
EaeI YGGCCR 1 cut(s) 126
EagI CGGCCG 1 cut(s) 126
Eam1104I CTCTTC 3 cut(s) 144, 575, 778
EarI CTCTTC 3 cut(s) 144, 575, 778
Ecl136II GAGCTC 2 cut(s) 417, 438
EclXI CGGCCG 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 1143
Eco147I AGGCCT 1 cut(s) 749
Eco24I GRGCYC 4 cut(s) 32, 275, 419, 440
Eco32I GATATC 1 cut(s) 942
Eco52I CGGCCG 1 cut(s) 126
Eco53kI GAGCTC 2 cut(s) 417, 438
Eco88I CYCGRG 1 cut(s) 433
EcoICRI GAGCTC 2 cut(s) 417, 438
EcoRII CCWGG 1 cut(s) 913
EcoRV GATATC 1 cut(s) 942
EcoT14I CCWWGG 1 cut(s) 1143
EcoT38I GRGCYC 4 cut(s) 32, 275, 419, 440
ErhI CCWWGG 1 cut(s) 1143
FaeI CATG 1 cut(s) 1147
FaiI YATR 7 cut(s) 134, 455, 545, 872, 1073, 1145, 1187
FaqI GGGAC 2 cut(s) 103, 158
FatI CATG 1 cut(s) 1143
FauI CCCGC 1 cut(s) 80
FbaI TGATCA 1 cut(s) 1096
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 9 cut(s) 126, 129, 564, 602, 696, 807, 948, 1038, 1173
FokI GGATG 4 cut(s) 482, 863, 1046, 1121
FriOI GRGCYC 4 cut(s) 32, 275, 419, 440
Fsp4HI GCNGC 9 cut(s) 126, 129, 564, 602, 696, 807, 948, 1038, 1173
FspBI CTAG 5 cut(s) 93, 951, 1104, 1125, 1152
GlaI GCGC 1 cut(s) 861
GluI GCNGC 9 cut(s) 126, 129, 564, 602, 696, 807, 948, 1038, 1173
GsuI CTGGAG 1 cut(s) 720
HaeIII GGCC 2 cut(s) 128, 749
HapII CCGG 3 cut(s) 138, 191, 275
HhaI GCGC 1 cut(s) 862
Hin1II CATG 1 cut(s) 1147
Hin6I GCGC 1 cut(s) 860
HinP1I GCGC 1 cut(s) 860
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 6 cut(s) 50, 114, 154, 442, 772, 898
HpaII CCGG 3 cut(s) 138, 191, 275
HphI GGTGA 2 cut(s) 16, 422
Hpy166II GTNNAC 3 cut(s) 181, 530, 634
Hpy188I TCNGA 6 cut(s) 71, 230, 248, 897, 1050, 1139
Hpy188III TCNNGA 6 cut(s) 160, 299, 716, 908, 970, 1152
Hpy8I GTNNAC 3 cut(s) 181, 530, 634
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 6 cut(s) 334, 340, 384, 400, 813, 931
HpyCH4III ACNGT 3 cut(s) 536, 631, 640
HpyCH4V TGCA 7 cut(s) 453, 597, 627, 809, 992, 1040, 1175
HpyF10VI GCNNNNNNNGC 8 cut(s) 84, 93, 134, 594, 701, 866, 1043, 1154
HpyF3I CTNAG 5 cut(s) 357, 464, 726, 894, 1049
Hsp92II CATG 1 cut(s) 1147
HspAI GCGC 1 cut(s) 860
Ksp22I TGATCA 1 cut(s) 1096
Kzo9I GATC 9 cut(s) 66, 218, 282, 557, 620, 799, 853, 910, 1096
LmnI GCTCC 2 cut(s) 193, 701
Lsp1109I GCAGC 7 cut(s) 550, 613, 707, 793, 959, 1024, 1159
LweI GCATC 3 cut(s) 979, 1024, 1157
MaeI CTAG 5 cut(s) 93, 951, 1104, 1125, 1152
MaeIII GTNAC 2 cut(s) 876, 1053
MalI GATC 9 cut(s) 68, 220, 284, 559, 622, 801, 855, 912, 1098
MboI GATC 9 cut(s) 66, 218, 282, 557, 620, 799, 853, 910, 1096
MfeI CAATTG 1 cut(s) 60
MflI RGATCY 3 cut(s) 620, 799, 910
MhlI GDGCHC 6 cut(s) 32, 275, 419, 434, 440, 590
MluCI AATT 3 cut(s) 60, 654, 957
MlyI GAGTC 1 cut(s) 163
MmeI TCCRAC 1 cut(s) 1022
MseI TTAA 1 cut(s) 830
MspA1I CMGCKG 1 cut(s) 698
MspI CCGG 3 cut(s) 138, 191, 275
MspR9I CCNGG 2 cut(s) 275, 915
MunI CAATTG 1 cut(s) 60
MvaI CCWGG 1 cut(s) 915
MwoI GCNNNNNNNGC 8 cut(s) 84, 93, 134, 594, 701, 866, 1043, 1154
NciI CCSGG 1 cut(s) 275
NcoI CCATGG 1 cut(s) 1143
NdeII GATC 9 cut(s) 66, 218, 282, 557, 620, 799, 853, 910, 1096
NlaIII CATG 1 cut(s) 1147
NlaIV GGNNCC 4 cut(s) 587, 703, 912, 919
NotI GCGGCCGC 1 cut(s) 126
PaeR7I CTCGAG 1 cut(s) 433
PceI AGGCCT 1 cut(s) 749
PfeI GAWTC 5 cut(s) 50, 114, 442, 772, 898
PfoI TCCNGGA 1 cut(s) 913
PkrI GCNGC 9 cut(s) 127, 130, 565, 603, 697, 808, 949, 1039, 1174
PleI GAGTC 1 cut(s) 162
PpsI GAGTC 1 cut(s) 162
Psp124BI GAGCTC 2 cut(s) 419, 440
Psp6I CCWGG 1 cut(s) 913
PspGI CCWGG 1 cut(s) 913
PspN4I GGNNCC 4 cut(s) 587, 703, 912, 919
PspXI VCTCGAGB 1 cut(s) 433
PstNI CAGNNNCTG 2 cut(s) 540, 664
PsuI RGATCY 3 cut(s) 620, 799, 910
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 1 cut(s) 517
RsaNI GTAC 1 cut(s) 516
SacI GAGCTC 2 cut(s) 419, 440
SalI GTCGAC 1 cut(s) 179
SaqAI TTAA 1 cut(s) 830
SatI GCNGC 9 cut(s) 126, 129, 564, 602, 696, 807, 948, 1038, 1173
Sau3AI GATC 9 cut(s) 66, 218, 282, 557, 620, 799, 853, 910, 1096
SchI GAGTC 1 cut(s) 163
ScrFI CCNGG 2 cut(s) 275, 915
SduI GDGCHC 6 cut(s) 32, 275, 419, 434, 440, 590
SfaNI GCATC 3 cut(s) 979, 1024, 1157
Sfr274I CTCGAG 1 cut(s) 433
SlaI CTCGAG 1 cut(s) 433
SmlI CTYRAG 2 cut(s) 433, 766
SmoI CTYRAG 2 cut(s) 433, 766
Sse9I AATT 3 cut(s) 60, 654, 957
SseBI AGGCCT 1 cut(s) 749
SsiI CCGC 6 cut(s) 87, 125, 129, 253, 566, 671
SspMI CTAG 5 cut(s) 93, 951, 1104, 1125, 1152
SstI GAGCTC 2 cut(s) 419, 440
StuI AGGCCT 1 cut(s) 749
StyD4I CCNGG 2 cut(s) 273, 913
StyI CCWWGG 1 cut(s) 1143
TaaI ACNGT 3 cut(s) 536, 631, 640
TaqI TCGA 5 cut(s) 180, 281, 434, 440, 928
TasI AATT 3 cut(s) 60, 654, 957
TauI GCSGC 2 cut(s) 128, 131
TfiI GAWTC 5 cut(s) 50, 114, 442, 772, 898
Tru1I TTAA 1 cut(s) 830
Tru9I TTAA 1 cut(s) 830
TscAI CASTG 1 cut(s) 294
TseI GCWGC 7 cut(s) 563, 601, 695, 806, 947, 1037, 1172
TspDTI ATGAA 3 cut(s) 390, 560, 855
TspRI CASTG 1 cut(s) 294
XapI RAATTY 1 cut(s) 957
XbaI TCTAGA 1 cut(s) 1151
XhoI CTCGAG 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 5 cut(s) 93, 951, 1104, 1125, 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.