RLG00000022247

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001421
Physical Location & Seq
Forward (+)
81919222 .. 81920930
1709 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022247

Sequence Viewer

Length: 1200 bp
ATGGTGAGAGAAGAACTGAAACAGAGCCCAAAAACTCAACCAAGAGAATCGAACCCAGTTGAGATCGGAAGTGGCCCAAGTGGGAACAGCTTGATGTTCAGTCCCAGATTCAAATCGGCGGCGGCCATGGCCGGTTGGGACGAAGAGTCTCTCCTGATCGCAAGCCTTGTCGTCGACGACACGCCGGAGCAAGATATTAAGCAGAAGAAACGATCTGTTTTCCGGTCCAAGACTCCACCCACCAATTCATCAAGAAGAAAGCGGAGAGTTCAGCGAAAGAGCCCGGTTTCGATCCCAGTGGCTGTTCTTGACCTTGATAGTGAAGAAACTACAAAAGAGGAGAGTGTGAAAGAGAAGGTAGAGCCTGAAATTGTTGTTGTGAATGAAGAAAAGAAAACAGGAGAAGATGGGAAGATGGGTGAGAGCTCTGGTGCTTCGTGCTCAAGCTCGAATCTTCCTTGCATGGATAAGCTTAGGGATGAGCTTTCTTGTGCTATCTGCTTGGAGATTTGCTTTGAACCAAGTACCACTCCTTGTGGACACAGCTTCTGTAAGAAATGCTTGCGATCGGCTGCTGATAAATGTGGGAAGAGGTGCCCCAAATGCAGGCAGCTAATGAGCAATGGAAGATCTTGCACCGTGAACACGGTTCTATGGAACACGATTCAGCTTCTGTTTCCTCAAGAAGTTGAAGCAAGGAAATCAGCTGGAGCCTTGAATAGTCGTGAAGAAGGTGAGCGGGAAATCCCTGATAAGGCCTTTAGTAGTAGCAACTCGAGGAATCAAAGCAGAAGAGTATCTGGCAGAGATGTGGCTGTAAGGAGAAGAAGGGTAGTGTTGAGCCAAGATGATGATGAGGACGATCCTGCACCACTAGCTATAAGGTTACAAATGAGAGAAGCTGAGAATCAAAATAGAGAACTAGGCAGCTTGGCTCGACCAGTAAGGATATCATCAGGCAGAGAAATTAGGCCTGTAAGGAGGGTATCAAGCAGAGATGCAAACCCGACGAGTAGTAGAAGGGTGCCAAACCAACATGAGGATGCTGCATTAGCTCTGAGATTACAGAGGGAGGAGTTTATGGAGGCTTTTAGGGGAACCAGTGATCAATCAAGTGCCTCTCTTTCGTTGGCTAGAGCAAACCTGAGAGCCATGGCATCTAGAGCCACTAACCGTGGTGCTGCAACCCGATTTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-C3HC4_2

Protein Analysis

400

Amino Acids

44.33

Weight (kDa)

9.31

Isoelectric Point (pI)

73.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4 PF00097 164 - 202 3.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 164 - 200 1.1e-06 RING-type zinc-finger
zf-C3HC4_2 PF13923 164 - 202 9.9e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 594, 1024
AccBSI CCGCTC 1 cut(s) 739
AccI GTMKAC 1 cut(s) 174
AciI CCGC 4 cut(s) 119, 122, 262, 739
AclWI GGATC 2 cut(s) 286, 857
AcoI YGGCCR 2 cut(s) 123, 129
AfaI GTAC 1 cut(s) 526
AfiI CCNNNNNNNGG 3 cut(s) 606, 754, 1039
AgsI TTSAA 4 cut(s) 112, 518, 692, 718
AhdI GACNNNNNGTC 1 cut(s) 145
Alw21I GWGCWC 2 cut(s) 428, 443
Alw26I GTCTC 1 cut(s) 153
AlwI GGATC 2 cut(s) 286, 857
AlwNI CAGNNNCTG 3 cut(s) 302, 549, 673
Ama87I CYCGRG 1 cut(s) 775
AoxI GGCC 5 cut(s) 73, 123, 129, 756, 971
ApeKI GCWGC 5 cut(s) 572, 610, 927, 1046, 1181
AspS9I GGNCC 2 cut(s) 74, 225
AsuC2I CCSGG 1 cut(s) 284
AsuHPI GGTGA 3 cut(s) 16, 431, 746
AvaI CYCGRG 1 cut(s) 775
AvaII GGWCC 1 cut(s) 225
BaeGI GKGCMC 1 cut(s) 599
BanI GGYRCC 2 cut(s) 594, 1024
BanII GRGCYC 3 cut(s) 29, 284, 428
Bbv12I GWGCWC 2 cut(s) 428, 443
BbvI GCAGC 5 cut(s) 559, 622, 939, 1033, 1168
BccI CCATC 2 cut(s) 401, 409
BclI TGATCA 1 cut(s) 1105
BcnI CCSGG 1 cut(s) 284
BcoDI GTCTC 1 cut(s) 153
BfaI CTAG 4 cut(s) 875, 923, 1134, 1161
BglII AGATCT 1 cut(s) 629
BisI GCNGC 7 cut(s) 120, 123, 573, 611, 928, 1047, 1182
BlsI GCNGC 7 cut(s) 121, 124, 574, 612, 929, 1048, 1183
Bme1390I CCNGG 1 cut(s) 284
Bme18I GGWCC 1 cut(s) 225
BmeRI GACNNNNNGTC 1 cut(s) 145
BmeT110I CYCGRG 1 cut(s) 775
BmgT120I GGNCC 2 cut(s) 74, 225
BmiI GGNNCC 4 cut(s) 596, 712, 1026, 1099
BmrFI CCNGG 1 cut(s) 284
BmrI ACTGGG 2 cut(s) 50, 290
BmsI GCATC 3 cut(s) 988, 1033, 1166
BmuI ACTGGG 2 cut(s) 50, 290
BpmI CTGGAG 1 cut(s) 729
Bpu10I CCTNAGC 1 cut(s) 473
BpuEI CTTGAG 2 cut(s) 427, 666
BpuMI CCSGG 1 cut(s) 284
BsaBI GATNNNNATC 1 cut(s) 112
BsaJI CCNNGG 3 cut(s) 126, 1152, 1174
BsaWI WCCGGW 1 cut(s) 222
Bsc4I CCNNNNNNNGG 3 cut(s) 606, 754, 1039
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 4 cut(s) 56, 296, 941, 1101
Bse3DI GCAATG 1 cut(s) 628
Bse8I GATNNNNATC 1 cut(s) 112
BseDI CCNNGG 3 cut(s) 126, 1152, 1174
BseGI GGATG 2 cut(s) 484, 1048
BseJI GATNNNNATC 1 cut(s) 112
BseLI CCNNNNNNNGG 3 cut(s) 606, 754, 1039
BseMI GCAATG 1 cut(s) 628
BseMII CTCAG 3 cut(s) 894, 1049, 1136
BseNI ACTGG 4 cut(s) 56, 296, 941, 1101
BseRI GAGGAG 2 cut(s) 353, 1088
BseSI GKGCMC 1 cut(s) 599
BseXI GCAGC 5 cut(s) 559, 622, 939, 1033, 1168
BsgI GTGCAG 1 cut(s) 852
Bsh1285I CGRYCG 1 cut(s) 569
BshFI GGCC 5 cut(s) 75, 125, 131, 758, 973
BshNI GGYRCC 2 cut(s) 594, 1024
BsiEI CGRYCG 1 cut(s) 569
BsiHKAI GWGCWC 2 cut(s) 428, 443
BsiHKCI CYCGRG 1 cut(s) 775
BsiSI CCGG 4 cut(s) 132, 185, 223, 284
BslFI GGGAC 2 cut(s) 87, 152
BslI CCNNNNNNNGG 3 cut(s) 606, 754, 1039
BsmAI GTCTC 1 cut(s) 153
BsmFI GGGAC 2 cut(s) 87, 152
BsnI GGCC 5 cut(s) 75, 125, 131, 758, 973
BsoBI CYCGRG 1 cut(s) 775
Bsp1286I GDGCHC 5 cut(s) 29, 284, 428, 443, 599
Bsp143I GATC 8 cut(s) 63, 156, 212, 291, 566, 629, 862, 1105
Bsp19I CCATGG 2 cut(s) 126, 1152
BspACI CCGC 4 cut(s) 119, 122, 262, 739
BspANI GGCC 5 cut(s) 75, 125, 131, 758, 973
BspCNI CTCAG 3 cut(s) 895, 1050, 1137
BspLI GGNNCC 4 cut(s) 596, 712, 1026, 1099
BspPI GGATC 2 cut(s) 286, 857
BspT107I GGYRCC 2 cut(s) 594, 1024
BsrBI CCGCTC 1 cut(s) 739
BsrDI GCAATG 1 cut(s) 628
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 4 cut(s) 56, 296, 941, 1101
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 3 cut(s) 126, 1152, 1174
BssMI GATC 8 cut(s) 63, 156, 212, 291, 566, 629, 862, 1105
BssT1I CCWWGG 2 cut(s) 126, 1152
Bst4CI ACNGT 3 cut(s) 640, 649, 1175
Bst6I CTCTTC 3 cut(s) 138, 584, 787
BstC8I GCNNGC 3 cut(s) 163, 563, 608
BstDEI CTNAG 4 cut(s) 473, 903, 1058, 1145
BstDSI CCRYGG 3 cut(s) 126, 1152, 1174
BstF5I GGATG 2 cut(s) 484, 1048
BstKTI GATC 8 cut(s) 66, 159, 215, 294, 569, 632, 865, 1108
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 8 cut(s) 63, 156, 212, 291, 566, 629, 862, 1105
BstMCI CGRYCG 1 cut(s) 569
BstMWI GCNNNNNNNGC 5 cut(s) 128, 603, 875, 1052, 1163
BstSCI CCNGG 1 cut(s) 282
BstSLI GKGCMC 1 cut(s) 599
BstV1I GCAGC 5 cut(s) 559, 622, 939, 1033, 1168
BstX2I RGATCY 1 cut(s) 629
BstYI RGATCY 1 cut(s) 629
BsuRI GGCC 5 cut(s) 75, 125, 131, 758, 973
BtgI CCRYGG 3 cut(s) 126, 1152, 1174
BtsCI GGATG 2 cut(s) 484, 1048
BtsIMutI CAGTG 2 cut(s) 303, 1108
Cac8I GCNNGC 3 cut(s) 163, 563, 608
CaiI CAGNNNCTG 3 cut(s) 302, 549, 673
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 74, 225
Csp6I GTAC 1 cut(s) 525
CviAII CATG 4 cut(s) 127, 463, 1037, 1153
CviQI GTAC 1 cut(s) 525
DdeI CTNAG 4 cut(s) 473, 903, 1058, 1145
DpnI GATC 8 cut(s) 65, 158, 214, 293, 568, 631, 864, 1107
DpnII GATC 8 cut(s) 63, 156, 212, 291, 566, 629, 862, 1105
DriI GACNNNNNGTC 1 cut(s) 145
EaeI YGGCCR 2 cut(s) 123, 129
Eam1104I CTCTTC 3 cut(s) 138, 584, 787
Eam1105I GACNNNNNGTC 1 cut(s) 145
EarI CTCTTC 3 cut(s) 138, 584, 787
Ecl136II GAGCTC 1 cut(s) 426
Eco130I CCWWGG 2 cut(s) 126, 1152
Eco147I AGGCCT 2 cut(s) 758, 973
Eco24I GRGCYC 3 cut(s) 29, 284, 428
Eco32I GATATC 1 cut(s) 951
Eco47I GGWCC 1 cut(s) 225
Eco53kI GAGCTC 1 cut(s) 426
Eco88I CYCGRG 1 cut(s) 775
EcoICRI GAGCTC 1 cut(s) 426
EcoRV GATATC 1 cut(s) 951
EcoT14I CCWWGG 2 cut(s) 126, 1152
EcoT38I GRGCYC 3 cut(s) 29, 284, 428
ErhI CCWWGG 2 cut(s) 126, 1152
FaeI CATG 4 cut(s) 130, 466, 1040, 1156
FaiI YATR 8 cut(s) 128, 464, 655, 881, 1038, 1082, 1154, 1196
FalI AAGNNNNNCTT 2 cut(s) 545, 577
FaqI GGGAC 2 cut(s) 87, 152
FatI CATG 4 cut(s) 126, 462, 1036, 1152
FauI CCCGC 1 cut(s) 732
FbaI TGATCA 1 cut(s) 1105
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 7 cut(s) 120, 123, 573, 611, 928, 1047, 1182
FokI GGATG 2 cut(s) 491, 1055
FriOI GRGCYC 3 cut(s) 29, 284, 428
Fsp4HI GCNGC 7 cut(s) 120, 123, 573, 611, 928, 1047, 1182
FspBI CTAG 4 cut(s) 875, 923, 1134, 1161
GluI GCNGC 7 cut(s) 120, 123, 573, 611, 928, 1047, 1182
GsuI CTGGAG 1 cut(s) 729
HaeIII GGCC 5 cut(s) 75, 125, 131, 758, 973
HapII CCGG 4 cut(s) 132, 185, 223, 284
Hin1II CATG 4 cut(s) 130, 466, 1040, 1156
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HindIII AAGCTT 1 cut(s) 470
HinfI GANTC 8 cut(s) 47, 108, 146, 232, 451, 664, 781, 907
HpaII CCGG 4 cut(s) 132, 185, 223, 284
HphI GGTGA 3 cut(s) 16, 431, 746
Hpy166II GTNNAC 3 cut(s) 175, 539, 643
Hpy188I TCNGA 2 cut(s) 68, 1059
Hpy188III TCNNGA 6 cut(s) 154, 252, 308, 683, 725, 1161
Hpy8I GTNNAC 3 cut(s) 175, 539, 643
Hpy99I CGWCG 3 cut(s) 176, 179, 1012
HpyAV CCTTC 4 cut(s) 349, 725, 822, 1014
HpyCH4III ACNGT 3 cut(s) 640, 649, 1175
HpyCH4V TGCA 7 cut(s) 462, 606, 636, 869, 1001, 1049, 1184
HpyF10VI GCNNNNNNNGC 5 cut(s) 128, 603, 875, 1052, 1163
HpyF3I CTNAG 4 cut(s) 473, 903, 1058, 1145
Hsp92II CATG 4 cut(s) 130, 466, 1040, 1156
Ksp22I TGATCA 1 cut(s) 1105
Kzo9I GATC 8 cut(s) 63, 156, 212, 291, 566, 629, 862, 1105
LmnI GCTCC 2 cut(s) 187, 710
Lsp1109I GCAGC 5 cut(s) 559, 622, 939, 1033, 1168
LweI GCATC 3 cut(s) 988, 1033, 1166
MaeI CTAG 4 cut(s) 875, 923, 1134, 1161
MaeIII GTNAC 1 cut(s) 885
MalI GATC 8 cut(s) 65, 158, 214, 293, 568, 631, 864, 1107
MbiI CCGCTC 1 cut(s) 739
MboI GATC 8 cut(s) 63, 156, 212, 291, 566, 629, 862, 1105
MflI RGATCY 1 cut(s) 629
MhlI GDGCHC 5 cut(s) 29, 284, 428, 443, 599
MluCI AATT 3 cut(s) 244, 369, 966
MlyI GAGTC 2 cut(s) 155, 226
MseI TTAA 1 cut(s) 198
MslI CAYNNNNRTG 1 cut(s) 1041
MspA1I CMGCKG 1 cut(s) 707
MspI CCGG 4 cut(s) 132, 185, 223, 284
MspR9I CCNGG 1 cut(s) 284
MwoI GCNNNNNNNGC 5 cut(s) 128, 603, 875, 1052, 1163
NciI CCSGG 1 cut(s) 284
NcoI CCATGG 2 cut(s) 126, 1152
NdeII GATC 8 cut(s) 63, 156, 212, 291, 566, 629, 862, 1105
NlaIII CATG 4 cut(s) 130, 466, 1040, 1156
NlaIV GGNNCC 4 cut(s) 596, 712, 1026, 1099
PaeR7I CTCGAG 1 cut(s) 775
PceI AGGCCT 2 cut(s) 758, 973
PfeI GAWTC 6 cut(s) 47, 108, 451, 664, 781, 907
PkrI GCNGC 7 cut(s) 121, 124, 574, 612, 929, 1048, 1183
Ple19I CGATCG 1 cut(s) 569
PleI GAGTC 2 cut(s) 154, 226
PpsI GAGTC 2 cut(s) 154, 226
Psp124BI GAGCTC 1 cut(s) 428
PspN4I GGNNCC 4 cut(s) 596, 712, 1026, 1099
PspPI GGNCC 2 cut(s) 74, 225
PspXI VCTCGAGB 1 cut(s) 775
PstNI CAGNNNCTG 3 cut(s) 302, 549, 673
PsuI RGATCY 1 cut(s) 629
PvuI CGATCG 1 cut(s) 569
PvuII CAGCTG 1 cut(s) 707
RsaI GTAC 1 cut(s) 526
RsaNI GTAC 1 cut(s) 525
RseI CAYNNNNRTG 1 cut(s) 1041
SacI GAGCTC 1 cut(s) 428
SalI GTCGAC 1 cut(s) 173
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 7 cut(s) 120, 123, 573, 611, 928, 1047, 1182
Sau3AI GATC 8 cut(s) 63, 156, 212, 291, 566, 629, 862, 1105
Sau96I GGNCC 2 cut(s) 74, 225
SchI GAGTC 2 cut(s) 155, 226
ScrFI CCNGG 1 cut(s) 284
SduI GDGCHC 5 cut(s) 29, 284, 428, 443, 599
SfaNI GCATC 3 cut(s) 988, 1033, 1166
Sfr274I CTCGAG 1 cut(s) 775
SgrDI CGTCGACG 1 cut(s) 173
SinI GGWCC 1 cut(s) 225
SlaI CTCGAG 1 cut(s) 775
SmiMI CAYNNNNRTG 1 cut(s) 1041
SmlI CTYRAG 3 cut(s) 442, 681, 775
SmoI CTYRAG 3 cut(s) 442, 681, 775
Sse9I AATT 3 cut(s) 244, 369, 966
SseBI AGGCCT 2 cut(s) 758, 973
SsiI CCGC 4 cut(s) 119, 122, 262, 739
SspMI CTAG 4 cut(s) 875, 923, 1134, 1161
SstI GAGCTC 1 cut(s) 428
StuI AGGCCT 2 cut(s) 758, 973
StyD4I CCNGG 1 cut(s) 282
StyI CCWWGG 2 cut(s) 126, 1152
TaaI ACNGT 3 cut(s) 640, 649, 1175
TaqI TCGA 6 cut(s) 50, 174, 290, 449, 776, 937
TasI AATT 3 cut(s) 244, 369, 966
TauI GCSGC 2 cut(s) 122, 125
TfiI GAWTC 6 cut(s) 47, 108, 451, 664, 781, 907
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TscAI CASTG 2 cut(s) 303, 1108
TseI GCWGC 5 cut(s) 572, 610, 927, 1046, 1181
TspDTI ATGAA 2 cut(s) 237, 399
TspRI CASTG 2 cut(s) 303, 1108
VpaK11BI GGWCC 1 cut(s) 225
XbaI TCTAGA 1 cut(s) 1160
XhoI CTCGAG 1 cut(s) 775
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 4 cut(s) 875, 923, 1134, 1161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.