pycom17g01530

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001421
Physical Location & Seq
Reverse (-)
1021509 .. 1024020
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g01530.3

Sequence Viewer

Length: 1308 bp
ATGAAGGAAGACTTCCTCTTTTGCATTGAATTCCAGCCGTTGGAAATTCAAAACCAAACACAAGATTCAAAACATTTGACCGTTAGCAAAAGGACCAAAATCCCTTACTCGTTAGCCTCTTTCTTTCCTGGAATTCGATTCTTCCCTTCCCCTTCGGTCCACCCAAAGGCACAACCAAAAATGGCGAGGAAGGAAGAAGAGCCAAAACAGAGTCCAATAAACCCGAAAAATCAGCAAAGAGAATCAGACCTAGTTGAAGTCGAAATCTGTGGCACCAGCGGCAATGCGATTGCATTCAGCCCCAGATTCAAATCCGTCGCCGCCATGGCCGGTTGGGACGAAGAGAGCCTTCTAGTCGCAAGCCTGGTGGTCGACGATACGCCGGAGAGAGATTTTAAACACAAGAAACGCTCTCGCAGAGCACCGAGAAGGAGCCCGATCTCAATCTCTGTAGCTGTCCTTGATCTTGATGATGCCGAAGAAACTGCGAAAGACGAGAGTGGGAAAGAGAAGAAAGTGGAATTGGAAGTTGTTGCGAATAAAGATAAGGAAACAAGAGAAGATAAAATGGCTGAGAGCTCGGGTGGTTCTTGCCCGCCAAGTACGAATCTTCCTTGCTTGGATAAGCTCAGGGAAGAACTTTCTTGCGCTATCTGCTTGGATATTTGCTTTGAACCAAGTACTACTCCTTGCGGTCACAGTTTCTGTAAGAAGTGCTTGCGGTCTGCTGCTGATAAATGTGGGAAGAGGTGCCCCAAGTGCAGGCAGCTAATCAGCAATAGTAGATCCTGCACCGTCAACACGGTTCTGTGGAACACAATACAGCTTCTGTTTCCACAAGAAGTTGAAGCAAGGAAAGCTGTTGGAGCCTCAAATGGCAGAGAAACTGATCGTCAAAGCCCTGGAAAGGCGGTATATAACGCAAGGAATGCTTTAAGTAGAAGGGCATCAGGGGCATCAAGCACAAGCAGCAGCGGAGAAATGACCATGAGGAGAAGACGGGTAATACTAAGCCAAGATGAAGAGGTTGAAGCTGAGCATCGAAACCAAGAACAAACTGCTGATAGAAGCATTCGGTTTTTAAGGGCATCAAGAAGAGAGATGAATGTGAGGATGAGTAGTAGAAGGATCCCAAGCCAGGATGAGGATGCTGCATTAGCTCTAAGATTGCAGAGGGAGGAGTTTATTGAGGCTTTTAGGGGAACCCCTGATCAGTCCAGTAGTTCTTTGATTTCTTCGGCAAGAGCAAACTTGAGAGCCATGGCATCTAGAGCTACTAACCATCGTCTTACCCGACATATGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-C3HC4_2

Protein Analysis

436

Amino Acids

48.8

Weight (kDa)

9.09

Isoelectric Point (pI)

72.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 272, 750
AccB7I CCANNNNNTGG 1 cut(s) 40
AccI GTMKAC 1 cut(s) 372
AciI CCGC 7 cut(s) 279, 321, 596, 693, 721, 911, 975
AclWI GGATC 3 cut(s) 780, 1123, 1136
AcoI YGGCCR 1 cut(s) 327
AcsI RAATTY 3 cut(s) 29, 45, 132
AfaI GTAC 2 cut(s) 604, 682
AfiI CCNNNNNNNGG 6 cut(s) 40, 166, 762, 907, 1138, 1144
AgsI TTSAA 8 cut(s) 29, 50, 69, 257, 310, 674, 848, 1031
AjnI CCWGG 4 cut(s) 127, 363, 901, 1137
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AluBI AGCT 9 cut(s) 455, 579, 628, 769, 826, 860, 1034, 1160, 1274
AluI AGCT 9 cut(s) 455, 579, 628, 769, 826, 860, 1034, 1160, 1274
Alw21I GWGCWC 2 cut(s) 424, 581
AlwI GGATC 3 cut(s) 780, 1123, 1136
AlwNI CAGNNNCTG 2 cut(s) 705, 829
Ama87I CYCGRG 1 cut(s) 580
AoxI GGCC 1 cut(s) 327
ApeKI GCWGC 5 cut(s) 728, 766, 969, 972, 1151
ApoI RAATTY 3 cut(s) 29, 45, 132
AspLEI GCGC 1 cut(s) 650
AspS9I GGNCC 2 cut(s) 93, 157
AvaI CYCGRG 1 cut(s) 580
AvaII GGWCC 2 cut(s) 93, 157
BaeGI GKGCMC 1 cut(s) 755
BamHI GGATCC 1 cut(s) 1128
BanI GGYRCC 2 cut(s) 272, 750
BanII GRGCYC 2 cut(s) 437, 581
BbsI GAAGAC 2 cut(s) 15, 1003
Bbv12I GWGCWC 2 cut(s) 424, 581
BbvI GCAGC 5 cut(s) 715, 778, 981, 984, 1138
BccI CCATC 1 cut(s) 1290
BceAI ACGGC 1 cut(s) 22
BcgI CGANNNNNNTGC 2 cut(s) 467, 501
BciT130I CCWGG 4 cut(s) 129, 365, 903, 1139
BclI TGATCA 1 cut(s) 1210
BfaI CTAG 3 cut(s) 251, 353, 1269
BfmI CTRYAG 1 cut(s) 450
BglI GCCNNNNNGGC 1 cut(s) 326
BisI GCNGC 7 cut(s) 280, 321, 729, 767, 970, 973, 1152
BlpI GCTNAGC 1 cut(s) 1035
BlsI GCNGC 7 cut(s) 281, 322, 730, 768, 971, 974, 1153
BmcAI AGTACT 1 cut(s) 682
Bme1390I CCNGG 4 cut(s) 129, 365, 903, 1139
Bme18I GGWCC 2 cut(s) 93, 157
BmeT110I CYCGRG 1 cut(s) 580
BmgT120I GGNCC 2 cut(s) 93, 157
BmiI GGNNCC 6 cut(s) 274, 434, 752, 868, 1130, 1204
BmrFI CCNGG 4 cut(s) 129, 365, 903, 1139
BmsI GCATC 7 cut(s) 463, 956, 965, 1048, 1097, 1138, 1274
BpiI GAAGAC 2 cut(s) 15, 1003
Bpu10I CCTNAGC 1 cut(s) 629
Bpu1102I GCTNAGC 1 cut(s) 1035
BpuEI CTTGAG 1 cut(s) 1273
BsaBI GATNNNNATC 2 cut(s) 310, 443
BsaJI CCNNGG 3 cut(s) 324, 901, 1260
Bsc4I CCNNNNNNNGG 6 cut(s) 40, 166, 762, 907, 1138, 1144
Bse118I RCCGGY 1 cut(s) 329
Bse1I ACTGG 1 cut(s) 1218
Bse3DI GCAATG 1 cut(s) 289
Bse8I GATNNNNATC 2 cut(s) 310, 443
BseBI CCWGG 4 cut(s) 129, 365, 903, 1139
BseDI CCNNGG 3 cut(s) 324, 901, 1260
BseGI GGATG 3 cut(s) 1119, 1147, 1153
BseJI GATNNNNATC 2 cut(s) 310, 443
BseLI CCNNNNNNNGG 6 cut(s) 40, 166, 762, 907, 1138, 1144
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 3 cut(s) 564, 643, 1026
BseNI ACTGG 1 cut(s) 1218
BseRI GAGGAG 2 cut(s) 1006, 1193
BseSI GKGCMC 1 cut(s) 755
BseXI GCAGC 5 cut(s) 715, 778, 981, 984, 1138
BsgI GTGCAG 2 cut(s) 775, 781
BshFI GGCC 1 cut(s) 329
BshNI GGYRCC 2 cut(s) 272, 750
BsiHKAI GWGCWC 2 cut(s) 424, 581
BsiHKCI CYCGRG 1 cut(s) 580
BsiSI CCGG 2 cut(s) 330, 383
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 6 cut(s) 40, 166, 762, 907, 1138, 1144
BsmFI GGGAC 1 cut(s) 350
BsmI GAATGC 3 cut(s) 293, 934, 1071
BsnI GGCC 1 cut(s) 329
BsoBI CYCGRG 1 cut(s) 580
Bsp1286I GDGCHC 4 cut(s) 424, 437, 581, 755
Bsp143I GATC 6 cut(s) 438, 463, 785, 889, 1128, 1210
Bsp1720I GCTNAGC 1 cut(s) 1035
Bsp19I CCATGG 2 cut(s) 324, 1260
BspACI CCGC 7 cut(s) 279, 321, 596, 693, 721, 911, 975
BspANI GGCC 1 cut(s) 329
BspCNI CTCAG 3 cut(s) 565, 642, 1027
BspLI GGNNCC 6 cut(s) 274, 434, 752, 868, 1130, 1204
BspPI GGATC 3 cut(s) 780, 1123, 1136
BspQI GCTCTTC 1 cut(s) 192
BspT107I GGYRCC 2 cut(s) 272, 750
BsrDI GCAATG 1 cut(s) 289
BsrFI RCCGGY 1 cut(s) 329
BsrI ACTGG 1 cut(s) 1218
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 3 cut(s) 324, 901, 1260
BssMI GATC 6 cut(s) 438, 463, 785, 889, 1128, 1210
BssT1I CCWWGG 2 cut(s) 324, 1260
Bst2UI CCWGG 4 cut(s) 129, 365, 903, 1139
Bst4CI ACNGT 4 cut(s) 82, 701, 796, 805
Bst6I CTCTTC 5 cut(s) 192, 336, 740, 1017, 1090
BstAPI GCANNNNNTGC 1 cut(s) 929
BstC8I GCNNGC 4 cut(s) 361, 596, 719, 764
BstDEI CTNAG 5 cut(s) 573, 629, 1010, 1035, 1163
BstDSI CCRYGG 2 cut(s) 324, 1260
BstF5I GGATG 3 cut(s) 1119, 1147, 1153
BstHHI GCGC 1 cut(s) 650
BstKTI GATC 6 cut(s) 441, 466, 788, 892, 1131, 1213
BstMBI GATC 6 cut(s) 438, 463, 785, 889, 1128, 1210
BstNI CCWGG 4 cut(s) 129, 365, 903, 1139
BstSCI CCNGG 4 cut(s) 127, 363, 901, 1137
BstSFI CTRYAG 1 cut(s) 450
BstSLI GKGCMC 1 cut(s) 755
BstV1I GCAGC 5 cut(s) 715, 778, 981, 984, 1138
BstV2I GAAGAC 2 cut(s) 15, 1003
BstX2I RGATCY 2 cut(s) 785, 1128
BstYI RGATCY 2 cut(s) 785, 1128
BsuRI GGCC 1 cut(s) 329
BtgI CCRYGG 2 cut(s) 324, 1260
BtsCI GGATG 3 cut(s) 1119, 1147, 1153
Cac8I GCNNGC 4 cut(s) 361, 596, 719, 764
CaiI CAGNNNCTG 2 cut(s) 705, 829
CfoI GCGC 1 cut(s) 650
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 2 cut(s) 93, 157
Csp6I GTAC 2 cut(s) 603, 681
CspCI CAANNNNNGTGG 2 cut(s) 348, 383
CviAII CATG 3 cut(s) 325, 988, 1261
CviQI GTAC 2 cut(s) 603, 681
DdeI CTNAG 5 cut(s) 573, 629, 1010, 1035, 1163
DpnI GATC 6 cut(s) 440, 465, 787, 891, 1130, 1212
DpnII GATC 6 cut(s) 438, 463, 785, 889, 1128, 1210
DraI TTTAAA 1 cut(s) 397
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 5 cut(s) 192, 336, 740, 1017, 1090
EarI CTCTTC 5 cut(s) 192, 336, 740, 1017, 1090
Ecl136II GAGCTC 1 cut(s) 579
Eco130I CCWWGG 2 cut(s) 324, 1260
Eco24I GRGCYC 2 cut(s) 437, 581
Eco47I GGWCC 2 cut(s) 93, 157
Eco53kI GAGCTC 1 cut(s) 579
Eco88I CYCGRG 1 cut(s) 580
EcoICRI GAGCTC 1 cut(s) 579
EcoRI GAATTC 2 cut(s) 29, 132
EcoRII CCWGG 4 cut(s) 127, 363, 901, 1137
EcoT14I CCWWGG 2 cut(s) 324, 1260
EcoT38I GRGCYC 2 cut(s) 437, 581
ErhI CCWWGG 2 cut(s) 324, 1260
FaeI CATG 3 cut(s) 328, 991, 1264
FaiI YATR 7 cut(s) 326, 916, 918, 989, 1262, 1299, 1301
FalI AAGNNNNNCTT 7 cut(s) 28, 333, 365, 701, 733, 916, 948
FaqI GGGAC 1 cut(s) 350
FatI CATG 3 cut(s) 324, 987, 1260
FauI CCCGC 1 cut(s) 603
FauNDI CATATG 1 cut(s) 1299
FbaI TGATCA 1 cut(s) 1210
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 7 cut(s) 280, 321, 729, 767, 970, 973, 1152
FokI GGATG 3 cut(s) 1126, 1154, 1160
FriOI GRGCYC 2 cut(s) 437, 581
Fsp4HI GCNGC 7 cut(s) 280, 321, 729, 767, 970, 973, 1152
FspBI CTAG 3 cut(s) 251, 353, 1269
GlaI GCGC 1 cut(s) 649
GluI GCNGC 7 cut(s) 280, 321, 729, 767, 970, 973, 1152
HaeIII GGCC 1 cut(s) 329
HapII CCGG 2 cut(s) 330, 383
HhaI GCGC 1 cut(s) 650
Hin1II CATG 3 cut(s) 328, 991, 1264
Hin6I GCGC 1 cut(s) 648
HinP1I GCGC 1 cut(s) 648
HincII GTYRAC 2 cut(s) 373, 799
HindII GTYRAC 2 cut(s) 373, 799
HinfI GANTC 6 cut(s) 65, 138, 211, 242, 306, 607
HpaII CCGG 2 cut(s) 330, 383
Hpy166II GTNNAC 3 cut(s) 160, 373, 799
Hpy188I TCNGA 1 cut(s) 247
Hpy188III TCNNGA 3 cut(s) 467, 1092, 1269
Hpy8I GTNNAC 3 cut(s) 160, 373, 799
Hpy99I CGWCG 2 cut(s) 320, 377
HpyAV CCTTC 7 cut(s) 156, 162, 184, 359, 423, 936, 1119
HpyCH4III ACNGT 4 cut(s) 82, 701, 796, 805
HpyCH4V TGCA 6 cut(s) 24, 293, 762, 792, 1154, 1171
HpyF3I CTNAG 5 cut(s) 573, 629, 1010, 1035, 1163
Hsp92II CATG 3 cut(s) 328, 991, 1264
HspAI GCGC 1 cut(s) 648
Ksp22I TGATCA 1 cut(s) 1210
Kzo9I GATC 6 cut(s) 438, 463, 785, 889, 1128, 1210
LguI GCTCTTC 1 cut(s) 192
LmnI GCTCC 2 cut(s) 432, 866
Lsp1109I GCAGC 5 cut(s) 715, 778, 981, 984, 1138
LweI GCATC 7 cut(s) 463, 956, 965, 1048, 1097, 1138, 1274
MaeI CTAG 3 cut(s) 251, 353, 1269
MaeIII GTNAC 1 cut(s) 695
MalI GATC 6 cut(s) 440, 465, 787, 891, 1130, 1212
MboI GATC 6 cut(s) 438, 463, 785, 889, 1128, 1210
MflI RGATCY 2 cut(s) 785, 1128
MhlI GDGCHC 4 cut(s) 424, 437, 581, 755
MluCI AATT 4 cut(s) 29, 45, 132, 521
MlyI GAGTC 1 cut(s) 220
MmeI TCCRAC 2 cut(s) 21, 844
MseI TTAA 3 cut(s) 396, 935, 1082
MspA1I CMGCKG 2 cut(s) 279, 975
MspI CCGG 2 cut(s) 330, 383
MspR9I CCNGG 4 cut(s) 129, 365, 903, 1139
Mva1269I GAATGC 3 cut(s) 293, 934, 1071
MvaI CCWGG 4 cut(s) 129, 365, 903, 1139
NcoI CCATGG 2 cut(s) 324, 1260
NdeI CATATG 1 cut(s) 1299
NdeII GATC 6 cut(s) 438, 463, 785, 889, 1128, 1210
NlaIII CATG 3 cut(s) 328, 991, 1264
NlaIV GGNNCC 6 cut(s) 274, 434, 752, 868, 1130, 1204
NmuCI GTSAC 1 cut(s) 695
PciSI GCTCTTC 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 1291
PctI GAATGC 3 cut(s) 293, 934, 1071
PfeI GAWTC 5 cut(s) 65, 138, 242, 306, 607
PflMI CCANNNNNTGG 1 cut(s) 40
PfoI TCCNGGA 1 cut(s) 127
PkrI GCNGC 7 cut(s) 281, 322, 730, 768, 971, 974, 1153
PleI GAGTC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 219
Psp124BI GAGCTC 1 cut(s) 581
Psp6I CCWGG 4 cut(s) 127, 363, 901, 1137
PspGI CCWGG 4 cut(s) 127, 363, 901, 1137
PspN4I GGNNCC 6 cut(s) 274, 434, 752, 868, 1130, 1204
PspPI GGNCC 2 cut(s) 93, 157
PstNI CAGNNNCTG 2 cut(s) 705, 829
PsuI RGATCY 2 cut(s) 785, 1128
RsaI GTAC 2 cut(s) 604, 682
RsaNI GTAC 2 cut(s) 603, 681
SacI GAGCTC 1 cut(s) 581
SalI GTCGAC 1 cut(s) 371
SapI GCTCTTC 1 cut(s) 192
SaqAI TTAA 3 cut(s) 396, 935, 1082
SatI GCNGC 7 cut(s) 280, 321, 729, 767, 970, 973, 1152
Sau3AI GATC 6 cut(s) 438, 463, 785, 889, 1128, 1210
Sau96I GGNCC 2 cut(s) 93, 157
ScaI AGTACT 1 cut(s) 682
SchI GAGTC 1 cut(s) 220
ScrFI CCNGG 4 cut(s) 129, 365, 903, 1139
SduI GDGCHC 4 cut(s) 424, 437, 581, 755
SfaNI GCATC 7 cut(s) 463, 956, 965, 1048, 1097, 1138, 1274
SfcI CTRYAG 1 cut(s) 450
SinI GGWCC 2 cut(s) 93, 157
SmlI CTYRAG 1 cut(s) 1252
SmoI CTYRAG 1 cut(s) 1252
Sse9I AATT 4 cut(s) 29, 45, 132, 521
SsiI CCGC 7 cut(s) 279, 321, 596, 693, 721, 911, 975
SspMI CTAG 3 cut(s) 251, 353, 1269
SstI GAGCTC 1 cut(s) 581
StyD4I CCNGG 4 cut(s) 127, 363, 901, 1137
StyI CCWWGG 2 cut(s) 324, 1260
TaaI ACNGT 4 cut(s) 82, 701, 796, 805
TaqI TCGA 4 cut(s) 136, 261, 372, 1042
TaqII GACCGA 1 cut(s) 145
TasI AATT 4 cut(s) 29, 45, 132, 521
TatI WGTACW 1 cut(s) 680
TauI GCSGC 2 cut(s) 282, 323
TfiI GAWTC 5 cut(s) 65, 138, 242, 306, 607
Tru1I TTAA 3 cut(s) 396, 935, 1082
Tru9I TTAA 3 cut(s) 396, 935, 1082
TseFI GTSAC 1 cut(s) 695
TseI GCWGC 5 cut(s) 728, 766, 969, 972, 1151
Tsp45I GTSAC 1 cut(s) 695
TspDTI ATGAA 3 cut(s) 17, 1035, 1118
TspGWI ACGGA 1 cut(s) 304
Van91I CCANNNNNTGG 1 cut(s) 40
VpaK11BI GGWCC 2 cut(s) 93, 157
XapI RAATTY 3 cut(s) 29, 45, 132
XbaI TCTAGA 1 cut(s) 1268
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 3 cut(s) 251, 353, 1269
ZrmI AGTACT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.