pycom111g01480

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0001421
Physical Location & Seq
Reverse (-)
941819 .. 943572
1754 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01480.4

Sequence Viewer

Length: 1173 bp
ATGGCGAGGAAAGAAGAAGAGCCGAAACAGAGTCCAATAAACCCGAAAAACCAGCAAAGAAAATCAGGCCCAGTTGAAATCGAAACCAATGGCACCAGCGGCAATGCTATTATATTCAGCCCCAGATTCAAATCCGTGGCCGCCATGGCCGGTTGGGACGAAGAGAGCCTTCTAATTGCAAGCCTGGTGGTTGATGATACGCCGGACAGAGATTTTAAACACAAGAAACGCTCCGTCTTGGGCTCCAAGACCCCACCCACCAATTCATCAAGAAGAAAGCGCAGAGCTCAGAGAAGGAGCCCGATTTCAATCCCTGTAGCTGTTCTTGATCTTGATGATGCAGAAGAAACTGCAAGGGACGAGACGGAGAAAGAGAAGAAGATGAAGCAGAAAATTGCTGCGAATAAAGAGAAGGAAACGAGAGAAGATAAAATGGCTGAGAGCTCTGGTGGTTCTTGCCCGCCAAGGTCGGAACTTCCTTGCTTGGATAAGCTCAGGGAAGAACTTTCTTGCGCTATCTGCTTGGAGATTTGCTTTGAACCAAGTACTACTCCTTGTGGCCACAGTTTCTGTAAGAAATGCTTACGGTCCGCCGCTGATAAATGTGGGAAGAGGTGCCCCAAGTGCAGGCAGCTAATCAGCAATAGTAGATCCTGCACCGTCAACACGGTTCTTTGGAACACGATACAGCTTCTGTTTCCGCAAGAAGTTGAAGCAAGGAAAGCTGTTGGAGCCTCAAATGGTAGAGAAACTGCTCGTCAAAGCCCCGGAAAGTCTGTGTATAACACAAGGAATGTTCAAAGTAGAAGGGCATCGGGGGTATCAAGCAGCAGCAGCAGCAGAGAAATGACCATGAGGAGAAGACGGGTGATATTAACCCAAGATGAAGAGGACGAACCTGAGCATCGGAACCCAGAACAAGGCGCTGATAGAAGTATTCGGTTTATAAGGGCATCAAGCGGAGAGATGAATGTGAGGAGTAGTAGTAGAAGGATCCCAAGCCAAGACGAGGATGCTGCATTGGCTCTAAGATTGCAGAGAGAGGAGTTTATGGACGCTTTTAGGGGAACCCCTGATGAATCCAGTAGTTCTTGGATTTCTTCGGCGAGAGCAAACATGAGAGCCATGGCATCCAGAGCTACTAACCACCGTCTTACCCGACATATGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-C3HC4_2

Protein Analysis

391

Amino Acids

43.73

Weight (kDa)

9.52

Isoelectric Point (pI)

78.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 947
AccB1I GGYRCC 2 cut(s) 92, 615
AciI CCGC 7 cut(s) 99, 141, 461, 591, 594, 701, 960
AclWI GGATC 3 cut(s) 645, 988, 1001
AcoI YGGCCR 3 cut(s) 138, 147, 559
AfaI GTAC 1 cut(s) 547
AfiI CCNNNNNNNGG 3 cut(s) 627, 920, 1009
AgsI TTSAA 6 cut(s) 77, 130, 309, 539, 713, 800
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 8 cut(s) 287, 320, 444, 493, 634, 691, 725, 1139
AluI AGCT 8 cut(s) 287, 320, 444, 493, 634, 691, 725, 1139
Alw21I GWGCWC 2 cut(s) 289, 446
Alw26I GTCTC 1 cut(s) 356
AlwI GGATC 3 cut(s) 645, 988, 1001
AlwNI CAGNNNCTG 2 cut(s) 570, 694
AoxI GGCC 4 cut(s) 67, 138, 147, 559
ApeKI GCWGC 7 cut(s) 398, 631, 828, 831, 834, 837, 1016
AspLEI GCGC 3 cut(s) 282, 515, 926
AspS9I GGNCC 2 cut(s) 68, 588
AsuC2I CCSGG 1 cut(s) 768
AsuHPI GGTGA 1 cut(s) 880
AvaII GGWCC 1 cut(s) 588
BaeGI GKGCMC 1 cut(s) 620
BalI TGGCCA 1 cut(s) 561
BamHI GGATCC 1 cut(s) 993
BanI GGYRCC 2 cut(s) 92, 615
BanII GRGCYC 4 cut(s) 245, 289, 302, 446
BbsI GAAGAC 1 cut(s) 868
Bbv12I GWGCWC 2 cut(s) 289, 446
BbvI GCAGC 7 cut(s) 385, 643, 840, 843, 846, 849, 1003
BcgI CGANNNNNNTGC 2 cut(s) 998, 1032
BciT130I CCWGG 1 cut(s) 185
BcnI CCSGG 1 cut(s) 768
BcoDI GTCTC 1 cut(s) 356
BfmI CTRYAG 1 cut(s) 315
BfoI RGCGCY 1 cut(s) 927
BglI GCCNNNNNGGC 1 cut(s) 146
BmcAI AGTACT 1 cut(s) 547
Bme1390I CCNGG 2 cut(s) 185, 768
Bme18I GGWCC 1 cut(s) 588
BmgT120I GGNCC 2 cut(s) 68, 588
BmiI GGNNCC 8 cut(s) 94, 244, 299, 617, 733, 911, 995, 1069
BmrFI CCNGG 2 cut(s) 185, 768
BmrI ACTGGG 1 cut(s) 65
BmsI GCATC 6 cut(s) 328, 821, 913, 962, 1003, 1139
BmuI ACTGGG 1 cut(s) 65
BpiI GAAGAC 1 cut(s) 868
Bpu10I CCTNAGC 2 cut(s) 494, 900
BpuMI CCSGG 1 cut(s) 768
BsaBI GATNNNNATC 2 cut(s) 130, 308
BsaJI CCNNGG 5 cut(s) 135, 144, 464, 766, 1125
BsaXI ACNNNNNCTCC 2 cut(s) 970, 1000
Bsc4I CCNNNNNNNGG 3 cut(s) 627, 920, 1009
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 2 cut(s) 71, 1083
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 2 cut(s) 130, 308
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 5 cut(s) 135, 144, 464, 766, 1125
BseGI GGATG 2 cut(s) 1018, 1130
BseJI GATNNNNATC 2 cut(s) 130, 308
BseLI CCNNNNNNNGG 3 cut(s) 627, 920, 1009
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 4 cut(s) 302, 429, 508, 891
BseNI ACTGG 2 cut(s) 71, 1083
BseRI GAGGAG 3 cut(s) 871, 991, 1058
BseSI GKGCMC 1 cut(s) 620
BseXI GCAGC 7 cut(s) 385, 643, 840, 843, 846, 849, 1003
BsgI GTGCAG 2 cut(s) 640, 646
BshFI GGCC 4 cut(s) 69, 140, 149, 561
BshNI GGYRCC 2 cut(s) 92, 615
BsiHKAI GWGCWC 2 cut(s) 289, 446
BsiSI CCGG 3 cut(s) 150, 203, 768
BslFI GGGAC 2 cut(s) 170, 371
BslI CCNNNNNNNGG 3 cut(s) 627, 920, 1009
BsmAI GTCTC 1 cut(s) 356
BsmBI CGTCTC 1 cut(s) 356
BsmFI GGGAC 2 cut(s) 170, 371
BsnI GGCC 4 cut(s) 69, 140, 149, 561
Bsp1286I GDGCHC 5 cut(s) 245, 289, 302, 446, 620
Bsp143I GATC 3 cut(s) 328, 650, 993
Bsp19I CCATGG 2 cut(s) 144, 1125
BspACI CCGC 7 cut(s) 99, 141, 461, 591, 594, 701, 960
BspANI GGCC 4 cut(s) 69, 140, 149, 561
BspCNI CTCAG 4 cut(s) 301, 430, 507, 892
BspLI GGNNCC 8 cut(s) 94, 244, 299, 617, 733, 911, 995, 1069
BspPI GGATC 3 cut(s) 645, 988, 1001
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 2 cut(s) 92, 615
BsrDI GCAATG 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 2 cut(s) 71, 1083
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 5 cut(s) 135, 144, 464, 766, 1125
BssMI GATC 3 cut(s) 328, 650, 993
BssT1I CCWWGG 3 cut(s) 144, 464, 1125
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 5 cut(s) 566, 588, 661, 670, 1151
Bst6I CTCTTC 4 cut(s) 12, 156, 605, 882
BstC8I GCNNGC 3 cut(s) 181, 461, 629
BstDEI CTNAG 5 cut(s) 288, 438, 494, 900, 1028
BstDSI CCRYGG 3 cut(s) 135, 144, 1125
BstF5I GGATG 2 cut(s) 1018, 1130
BstH2I RGCGCY 1 cut(s) 927
BstHHI GCGC 3 cut(s) 282, 515, 926
BstKTI GATC 3 cut(s) 331, 653, 996
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 3 cut(s) 328, 650, 993
BstNI CCWGG 1 cut(s) 185
BstSCI CCNGG 2 cut(s) 183, 766
BstSFI CTRYAG 1 cut(s) 315
BstSLI GKGCMC 1 cut(s) 620
BstV1I GCAGC 7 cut(s) 385, 643, 840, 843, 846, 849, 1003
BstV2I GAAGAC 1 cut(s) 868
BstX2I RGATCY 2 cut(s) 650, 993
BstYI RGATCY 2 cut(s) 650, 993
BsuRI GGCC 4 cut(s) 69, 140, 149, 561
BtgI CCRYGG 3 cut(s) 135, 144, 1125
BtsCI GGATG 2 cut(s) 1018, 1130
Cac8I GCNNGC 3 cut(s) 181, 461, 629
CaiI CAGNNNCTG 2 cut(s) 570, 694
CfoI GCGC 3 cut(s) 282, 515, 926
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 68, 588
CpoI CGGWCCG 1 cut(s) 588
CseI GACGC 1 cut(s) 1064
Csp6I GTAC 1 cut(s) 546
CspCI CAANNNNNGTGG 2 cut(s) 168, 203
CspI CGGWCCG 1 cut(s) 588
CviAII CATG 4 cut(s) 145, 853, 1117, 1126
CviQI GTAC 1 cut(s) 546
DdeI CTNAG 5 cut(s) 288, 438, 494, 900, 1028
DpnI GATC 3 cut(s) 330, 652, 995
DpnII GATC 3 cut(s) 328, 650, 993
DraI TTTAAA 1 cut(s) 217
EaeI YGGCCR 3 cut(s) 138, 147, 559
Eam1104I CTCTTC 4 cut(s) 12, 156, 605, 882
EarI CTCTTC 4 cut(s) 12, 156, 605, 882
EciI GGCGGA 1 cut(s) 580
Ecl136II GAGCTC 2 cut(s) 287, 444
Eco130I CCWWGG 3 cut(s) 144, 464, 1125
Eco24I GRGCYC 4 cut(s) 245, 289, 302, 446
Eco47I GGWCC 1 cut(s) 588
Eco53kI GAGCTC 2 cut(s) 287, 444
EcoICRI GAGCTC 2 cut(s) 287, 444
EcoRII CCWGG 1 cut(s) 183
EcoT14I CCWWGG 3 cut(s) 144, 464, 1125
EcoT38I GRGCYC 4 cut(s) 245, 289, 302, 446
ErhI CCWWGG 3 cut(s) 144, 464, 1125
Esp3I CGTCTC 1 cut(s) 356
FaeI CATG 4 cut(s) 148, 856, 1120, 1129
FalI AAGNNNNNCTT 4 cut(s) 153, 185, 566, 598
FaqI GGGAC 2 cut(s) 170, 371
FatI CATG 4 cut(s) 144, 852, 1116, 1125
FauI CCCGC 1 cut(s) 468
FauNDI CATATG 1 cut(s) 1164
FokI GGATG 2 cut(s) 1025, 1117
FriOI GRGCYC 4 cut(s) 245, 289, 302, 446
GlaI GCGC 3 cut(s) 281, 514, 925
HaeII RGCGCY 1 cut(s) 927
HaeIII GGCC 4 cut(s) 69, 140, 149, 561
HapII CCGG 3 cut(s) 150, 203, 768
HgaI GACGC 1 cut(s) 1064
HhaI GCGC 3 cut(s) 282, 515, 926
Hin1II CATG 4 cut(s) 148, 856, 1120, 1129
Hin6I GCGC 3 cut(s) 280, 513, 924
HinP1I GCGC 3 cut(s) 280, 513, 924
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HinfI GANTC 3 cut(s) 31, 126, 1079
HpaII CCGG 3 cut(s) 150, 203, 768
HphI GGTGA 1 cut(s) 880
Hpy166II GTNNAC 1 cut(s) 664
Hpy188I TCNGA 3 cut(s) 291, 472, 909
Hpy188III TCNNGA 4 cut(s) 270, 326, 332, 1134
Hpy8I GTNNAC 1 cut(s) 664
HpyAV CCTTC 5 cut(s) 179, 288, 406, 801, 984
HpyCH4III ACNGT 5 cut(s) 566, 588, 661, 670, 1151
HpyCH4V TGCA 7 cut(s) 179, 341, 353, 627, 657, 1019, 1036
HpyF3I CTNAG 5 cut(s) 288, 438, 494, 900, 1028
Hsp92II CATG 4 cut(s) 148, 856, 1120, 1129
HspAI GCGC 3 cut(s) 280, 513, 924
Kzo9I GATC 3 cut(s) 328, 650, 993
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 4 cut(s) 236, 248, 297, 731
Lsp1109I GCAGC 7 cut(s) 385, 643, 840, 843, 846, 849, 1003
LweI GCATC 6 cut(s) 328, 821, 913, 962, 1003, 1139
MalI GATC 3 cut(s) 330, 652, 995
MboI GATC 3 cut(s) 328, 650, 993
MflI RGATCY 2 cut(s) 650, 993
MhlI GDGCHC 5 cut(s) 245, 289, 302, 446, 620
MlsI TGGCCA 1 cut(s) 561
MluCI AATT 3 cut(s) 174, 262, 393
MluNI TGGCCA 1 cut(s) 561
MlyI GAGTC 1 cut(s) 40
MmeI TCCRAC 2 cut(s) 450, 709
MnlI CCTC 7 cut(s) 606, 745, 849, 883, 969, 1003, 1036
Mox20I TGGCCA 1 cut(s) 561
MscI TGGCCA 1 cut(s) 561
MseI TTAA 2 cut(s) 216, 875
Msp20I TGGCCA 1 cut(s) 561
MspA1I CMGCKG 2 cut(s) 99, 596
MspI CCGG 3 cut(s) 150, 203, 768
MspR9I CCNGG 2 cut(s) 185, 768
MvaI CCWGG 1 cut(s) 185
NciI CCSGG 1 cut(s) 768
NcoI CCATGG 2 cut(s) 144, 1125
NdeI CATATG 1 cut(s) 1164
NdeII GATC 3 cut(s) 328, 650, 993
NlaIII CATG 4 cut(s) 148, 856, 1120, 1129
NlaIV GGNNCC 8 cut(s) 94, 244, 299, 617, 733, 911, 995, 1069
PciSI GCTCTTC 1 cut(s) 12
PfeI GAWTC 2 cut(s) 126, 1079
PleI GAGTC 1 cut(s) 39
PpsI GAGTC 1 cut(s) 39
PsiI TTATAA 1 cut(s) 947
Psp124BI GAGCTC 2 cut(s) 289, 446
Psp6I CCWGG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 8 cut(s) 94, 244, 299, 617, 733, 911, 995, 1069
PspPI GGNCC 2 cut(s) 68, 588
PstNI CAGNNNCTG 2 cut(s) 570, 694
PsuI RGATCY 2 cut(s) 650, 993
RsaI GTAC 1 cut(s) 547
RsaNI GTAC 1 cut(s) 546
Rsr2I CGGWCCG 1 cut(s) 588
RsrII CGGWCCG 1 cut(s) 588
SacI GAGCTC 2 cut(s) 289, 446
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 216, 875
Sau3AI GATC 3 cut(s) 328, 650, 993
Sau96I GGNCC 2 cut(s) 68, 588
ScaI AGTACT 1 cut(s) 547
SchI GAGTC 1 cut(s) 40
ScrFI CCNGG 2 cut(s) 185, 768
SduI GDGCHC 5 cut(s) 245, 289, 302, 446, 620
SfaNI GCATC 6 cut(s) 328, 821, 913, 962, 1003, 1139
SfcI CTRYAG 1 cut(s) 315
SfiI GGCCNNNNNGGCC 1 cut(s) 146
SinI GGWCC 1 cut(s) 588
Sse9I AATT 3 cut(s) 174, 262, 393
SsiI CCGC 7 cut(s) 99, 141, 461, 591, 594, 701, 960
SstI GAGCTC 2 cut(s) 289, 446
StyD4I CCNGG 2 cut(s) 183, 766
StyI CCWWGG 3 cut(s) 144, 464, 1125
TaaI ACNGT 5 cut(s) 566, 588, 661, 670, 1151
TaqI TCGA 1 cut(s) 81
TasI AATT 3 cut(s) 174, 262, 393
TatI WGTACW 1 cut(s) 545
TauI GCSGC 3 cut(s) 102, 143, 596
TfiI GAWTC 2 cut(s) 126, 1079
Tru1I TTAA 2 cut(s) 216, 875
Tru9I TTAA 2 cut(s) 216, 875
TseI GCWGC 7 cut(s) 398, 631, 828, 831, 834, 837, 1016
TspDTI ATGAA 5 cut(s) 255, 398, 900, 983, 1092
TspGWI ACGGA 3 cut(s) 124, 223, 380
VpaK11BI GGWCC 1 cut(s) 588
ZrmI AGTACT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.