MD17G1018600.v1.1

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001421
Physical Location & Seq
Reverse (-)
1454654 .. 1456778
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1018600.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGGCGAGGAAAGAAGAAGAGCCAAAACAGAGTCCGATAAACCCGAAAAACCAGCAAAGAGAACCAGACCCAGTTGAAGTCGAAATCAGTGGCACCAGCGGCAATGCGATTATATTCAGCCCCAGATTCAAATCCGTCGCCGCCATGGCCGGTTGGGACGAAGAGAGCCTTCTAGTCGCAAGCCTGGTGGTCGACGATACACCGGAGAGAGATTTTAAACACAAGAAACGCTCTGTCTTGAACTCCAAGACCCCACCCACCAATTCATCAAGAAGAAAGCGCAGAGCACCGAGAAGGAGCCCGATCTCAATCCCTGTAGCTGTCCTTGATCTTGATGATGCCGAAGAAACTGCGAAAGATGAGGGTGGGAAAGAGAAGAAGGTAGAACTGGAAATTTTTGCAAATAAAGATAAGGAAACAAGAGAAGATAAAATGGCTGAGAGCTCTGGTGGTTCTTGCCCGCCAAGTTCGAATCTTCCTTGCTTGGATAAGCTCAGAGAGGAACTTTCTTGCGCTATCTGCTTGGAGATTTGCTTTGAACCAAGTACTACTCCTTGTGGGCACAGTTTCTGTAAGAAGTGCTTGCGGTCCGCTGCTGATAAATGTGGGAAGAGGTGCCCTAAGTGCAGGCAGCTAATCAGCAATAGTAGATCCTGCACCGTCAATACGGTTCTTTGGAACACAATACAGCTTCTGTTTCCACAAGAAGTTGAAGCAAGGAAAGCTGTTGGAGCCTCAAATGGCAGAGAAACTGATCGTCAAAGCCCTGGAAAGGCGGTATATAACGCAAGGAATGCTCAAAGTAGAAGGGCATCGGGGGTATCAAGCACAAGCAGCAGCAGAGAAATGACCATGAGGAGAAGACGGGTAATACTAAGCCAAGATGAAGAGGATGAAGCGGAGCATCGAAACCAAGAACAAACTGCTGACAGAAGCATTCGGTTTTTAAGGGCATCAAGCAGAGAGATGTATTTGAGGAGTAGTAGTAGAAGGATCTCAAGCCAGGATGAGGATGCTGCATTAGTTCTGAGATTGCAGAGGGAGGAGTTTATGGAGGCTTTTAGGGGAACCCCTGATCAGTCCAGTAGTTCTTTGATTTCTTCGGCAAGAGCAAACATGAGAGCCCTGGCATCTAGAGCTACTAACCATCGTCTTACCCGACATAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-C3HC4_2

Protein Analysis

391

Amino Acids

43.7

Weight (kDa)

9.26

Isoelectric Point (pI)

79.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4 PF00097 171 - 209 3.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 171 - 209 9.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 171 - 207 1.2e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 92, 615
AccI GTMKAC 1 cut(s) 192
AciI CCGC 7 cut(s) 99, 141, 461, 586, 591, 776, 899
AclWI GGATC 2 cut(s) 645, 1001
AcoI YGGCCR 1 cut(s) 147
AcsI RAATTY 1 cut(s) 393
AfaI GTAC 1 cut(s) 547
AfiI CCNNNNNNNGG 2 cut(s) 772, 1009
AgsI TTSAA 5 cut(s) 77, 130, 241, 539, 713
AjnI CCWGG 4 cut(s) 183, 766, 1002, 1125
AluBI AGCT 7 cut(s) 320, 444, 493, 634, 691, 725, 1139
AluI AGCT 7 cut(s) 320, 444, 493, 634, 691, 725, 1139
Alw21I GWGCWC 2 cut(s) 289, 446
AlwI GGATC 2 cut(s) 645, 1001
AlwNI CAGNNNCTG 2 cut(s) 570, 694
AoxI GGCC 1 cut(s) 147
ApeKI GCWGC 5 cut(s) 593, 631, 834, 837, 1016
ApoI RAATTY 1 cut(s) 393
AspLEI GCGC 2 cut(s) 282, 515
AspS9I GGNCC 1 cut(s) 588
AsuII TTCGAA 1 cut(s) 470
AvaII GGWCC 1 cut(s) 588
BaeGI GKGCMC 2 cut(s) 564, 620
BanI GGYRCC 2 cut(s) 92, 615
BanII GRGCYC 3 cut(s) 302, 446, 1126
BbsI GAAGAC 1 cut(s) 868
Bbv12I GWGCWC 2 cut(s) 289, 446
BbvI GCAGC 5 cut(s) 580, 643, 846, 849, 1003
BccI CCATC 1 cut(s) 1155
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 4 cut(s) 185, 768, 1004, 1127
BclI TGATCA 1 cut(s) 1075
BfaI CTAG 2 cut(s) 173, 1134
BfmI CTRYAG 1 cut(s) 315
BglI GCCNNNNNGGC 1 cut(s) 146
BisI GCNGC 7 cut(s) 100, 141, 594, 632, 835, 838, 1017
BlsI GCNGC 7 cut(s) 101, 142, 595, 633, 836, 839, 1018
BmcAI AGTACT 1 cut(s) 547
Bme1390I CCNGG 4 cut(s) 185, 768, 1004, 1127
Bme18I GGWCC 1 cut(s) 588
BmgT120I GGNCC 1 cut(s) 588
BmiI GGNNCC 5 cut(s) 94, 299, 617, 733, 1069
BmrFI CCNGG 4 cut(s) 185, 768, 1004, 1127
BmrI ACTGGG 1 cut(s) 65
BmsI GCATC 6 cut(s) 328, 821, 913, 962, 1003, 1139
BmuI ACTGGG 1 cut(s) 65
BpiI GAAGAC 1 cut(s) 868
Bpu14I TTCGAA 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 982
BsaBI GATNNNNATC 2 cut(s) 130, 308
BsaJI CCNNGG 3 cut(s) 144, 766, 1125
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 970, 1000
Bsc4I CCNNNNNNNGG 2 cut(s) 772, 1009
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 3 cut(s) 71, 393, 1083
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 2 cut(s) 130, 308
BseBI CCWGG 4 cut(s) 185, 768, 1004, 1127
BseDI CCNNGG 3 cut(s) 144, 766, 1125
BseGI GGATG 3 cut(s) 898, 1012, 1018
BseJI GATNNNNATC 2 cut(s) 130, 308
BseLI CCNNNNNNNGG 2 cut(s) 772, 1009
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 3 cut(s) 429, 508, 1019
BseNI ACTGG 3 cut(s) 71, 393, 1083
BseRI GAGGAG 3 cut(s) 871, 991, 1058
BseSI GKGCMC 2 cut(s) 564, 620
BseXI GCAGC 5 cut(s) 580, 643, 846, 849, 1003
BsgI GTGCAG 2 cut(s) 640, 646
BshFI GGCC 1 cut(s) 149
BshNI GGYRCC 2 cut(s) 92, 615
BsiHKAI GWGCWC 2 cut(s) 289, 446
BsiSI CCGG 2 cut(s) 150, 203
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 2 cut(s) 772, 1009
BsmFI GGGAC 1 cut(s) 170
BsmI GAATGC 2 cut(s) 799, 936
BsnI GGCC 1 cut(s) 149
Bsp119I TTCGAA 1 cut(s) 470
Bsp1286I GDGCHC 6 cut(s) 289, 302, 446, 564, 620, 1126
Bsp143I GATC 6 cut(s) 303, 328, 650, 754, 993, 1075
Bsp19I CCATGG 1 cut(s) 144
BspACI CCGC 7 cut(s) 99, 141, 461, 586, 591, 776, 899
BspANI GGCC 1 cut(s) 149
BspCNI CTCAG 3 cut(s) 430, 507, 1020
BspLI GGNNCC 5 cut(s) 94, 299, 617, 733, 1069
BspPI GGATC 2 cut(s) 645, 1001
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 470
BspT107I GGYRCC 2 cut(s) 92, 615
BsrDI GCAATG 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 3 cut(s) 71, 393, 1083
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 3 cut(s) 144, 766, 1125
BssMI GATC 6 cut(s) 303, 328, 650, 754, 993, 1075
BssT1I CCWWGG 1 cut(s) 144
Bst2UI CCWGG 4 cut(s) 185, 768, 1004, 1127
Bst4CI ACNGT 3 cut(s) 566, 661, 670
Bst6I CTCTTC 4 cut(s) 12, 156, 605, 882
BstAPI GCANNNNNTGC 1 cut(s) 794
BstBI TTCGAA 1 cut(s) 470
BstC8I GCNNGC 4 cut(s) 181, 461, 584, 629
BstDEI CTNAG 5 cut(s) 438, 494, 621, 875, 1028
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 3 cut(s) 898, 1012, 1018
BstHHI GCGC 2 cut(s) 282, 515
BstKTI GATC 6 cut(s) 306, 331, 653, 757, 996, 1078
BstMBI GATC 6 cut(s) 303, 328, 650, 754, 993, 1075
BstMWI GCNNNNNNNGC 9 cut(s) 99, 146, 519, 624, 722, 731, 794, 834, 1136
BstNI CCWGG 4 cut(s) 185, 768, 1004, 1127
BstSCI CCNGG 4 cut(s) 183, 766, 1002, 1125
BstSFI CTRYAG 1 cut(s) 315
BstSLI GKGCMC 2 cut(s) 564, 620
BstV1I GCAGC 5 cut(s) 580, 643, 846, 849, 1003
BstV2I GAAGAC 1 cut(s) 868
BstX2I RGATCY 2 cut(s) 650, 993
BstYI RGATCY 2 cut(s) 650, 993
BsuRI GGCC 1 cut(s) 149
BtgI CCRYGG 1 cut(s) 144
BtsCI GGATG 3 cut(s) 898, 1012, 1018
BtsIMutI CAGTG 1 cut(s) 94
Cac8I GCNNGC 4 cut(s) 181, 461, 584, 629
CaiI CAGNNNCTG 2 cut(s) 570, 694
CfoI GCGC 2 cut(s) 282, 515
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 588
CpoI CGGWCCG 1 cut(s) 588
Csp6I GTAC 1 cut(s) 546
CspCI CAANNNNNGTGG 2 cut(s) 168, 203
CspI CGGWCCG 1 cut(s) 588
CviAII CATG 3 cut(s) 145, 853, 1117
CviQI GTAC 1 cut(s) 546
DdeI CTNAG 5 cut(s) 438, 494, 621, 875, 1028
DpnI GATC 6 cut(s) 305, 330, 652, 756, 995, 1077
DpnII GATC 6 cut(s) 303, 328, 650, 754, 993, 1075
DraI TTTAAA 1 cut(s) 217
EaeI YGGCCR 1 cut(s) 147
Eam1104I CTCTTC 4 cut(s) 12, 156, 605, 882
EarI CTCTTC 4 cut(s) 12, 156, 605, 882
Ecl136II GAGCTC 1 cut(s) 444
Eco130I CCWWGG 1 cut(s) 144
Eco24I GRGCYC 3 cut(s) 302, 446, 1126
Eco47I GGWCC 1 cut(s) 588
Eco53kI GAGCTC 1 cut(s) 444
EcoICRI GAGCTC 1 cut(s) 444
EcoRII CCWGG 4 cut(s) 183, 766, 1002, 1125
EcoT14I CCWWGG 1 cut(s) 144
EcoT38I GRGCYC 3 cut(s) 302, 446, 1126
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 3 cut(s) 148, 856, 1120
FaiI YATR 8 cut(s) 113, 146, 781, 783, 854, 1052, 1118, 1164
FalI AAGNNNNNCTT 4 cut(s) 153, 185, 566, 598
FaqI GGGAC 1 cut(s) 170
FatI CATG 3 cut(s) 144, 852, 1116
FauI CCCGC 1 cut(s) 468
FbaI TGATCA 1 cut(s) 1075
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 7 cut(s) 100, 141, 594, 632, 835, 838, 1017
FokI GGATG 3 cut(s) 905, 1019, 1025
FriOI GRGCYC 3 cut(s) 302, 446, 1126
Fsp4HI GCNGC 7 cut(s) 100, 141, 594, 632, 835, 838, 1017
FspBI CTAG 2 cut(s) 173, 1134
GlaI GCGC 2 cut(s) 281, 514
GluI GCNGC 7 cut(s) 100, 141, 594, 632, 835, 838, 1017
HaeIII GGCC 1 cut(s) 149
HapII CCGG 2 cut(s) 150, 203
HhaI GCGC 2 cut(s) 282, 515
Hin1II CATG 3 cut(s) 148, 856, 1120
Hin6I GCGC 2 cut(s) 280, 513
HinP1I GCGC 2 cut(s) 280, 513
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HinfI GANTC 3 cut(s) 31, 126, 472
HpaII CCGG 2 cut(s) 150, 203
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 3 cut(s) 36, 497, 1029
Hpy188III TCNNGA 4 cut(s) 238, 270, 332, 1134
Hpy8I GTNNAC 1 cut(s) 193
Hpy99I CGWCG 2 cut(s) 140, 197
HpyAV CCTTC 5 cut(s) 179, 288, 373, 801, 984
HpyCH4III ACNGT 3 cut(s) 566, 661, 670
HpyCH4V TGCA 5 cut(s) 401, 627, 657, 1019, 1036
HpyF10VI GCNNNNNNNGC 9 cut(s) 99, 146, 519, 624, 722, 731, 794, 834, 1136
HpyF3I CTNAG 5 cut(s) 438, 494, 621, 875, 1028
Hsp92II CATG 3 cut(s) 148, 856, 1120
HspAI GCGC 2 cut(s) 280, 513
Ksp22I TGATCA 1 cut(s) 1075
Kzo9I GATC 6 cut(s) 303, 328, 650, 754, 993, 1075
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 3 cut(s) 297, 731, 901
Lsp1109I GCAGC 5 cut(s) 580, 643, 846, 849, 1003
LweI GCATC 6 cut(s) 328, 821, 913, 962, 1003, 1139
MaeI CTAG 2 cut(s) 173, 1134
MalI GATC 6 cut(s) 305, 330, 652, 756, 995, 1077
MboI GATC 6 cut(s) 303, 328, 650, 754, 993, 1075
MflI RGATCY 2 cut(s) 650, 993
MhlI GDGCHC 6 cut(s) 289, 302, 446, 564, 620, 1126
MluCI AATT 2 cut(s) 262, 393
MlyI GAGTC 1 cut(s) 40
MmeI TCCRAC 1 cut(s) 709
MseI TTAA 2 cut(s) 216, 947
MspA1I CMGCKG 2 cut(s) 99, 593
MspI CCGG 2 cut(s) 150, 203
MspR9I CCNGG 4 cut(s) 185, 768, 1004, 1127
Mva1269I GAATGC 2 cut(s) 799, 936
MvaI CCWGG 4 cut(s) 185, 768, 1004, 1127
MwoI GCNNNNNNNGC 9 cut(s) 99, 146, 519, 624, 722, 731, 794, 834, 1136
NcoI CCATGG 1 cut(s) 144
NdeII GATC 6 cut(s) 303, 328, 650, 754, 993, 1075
NlaIII CATG 3 cut(s) 148, 856, 1120
NlaIV GGNNCC 5 cut(s) 94, 299, 617, 733, 1069
NspV TTCGAA 1 cut(s) 470
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 1156
PctI GAATGC 2 cut(s) 799, 936
PfeI GAWTC 2 cut(s) 126, 472
PkrI GCNGC 7 cut(s) 101, 142, 595, 633, 836, 839, 1018
PleI GAGTC 1 cut(s) 39
PpsI GAGTC 1 cut(s) 39
Psp124BI GAGCTC 1 cut(s) 446
Psp6I CCWGG 4 cut(s) 183, 766, 1002, 1125
PspGI CCWGG 4 cut(s) 183, 766, 1002, 1125
PspN4I GGNNCC 5 cut(s) 94, 299, 617, 733, 1069
PspPI GGNCC 1 cut(s) 588
PstNI CAGNNNCTG 2 cut(s) 570, 694
PsuI RGATCY 2 cut(s) 650, 993
RsaI GTAC 1 cut(s) 547
RsaNI GTAC 1 cut(s) 546
Rsr2I CGGWCCG 1 cut(s) 588
RsrII CGGWCCG 1 cut(s) 588
SacI GAGCTC 1 cut(s) 446
SalI GTCGAC 1 cut(s) 191
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 216, 947
SatI GCNGC 7 cut(s) 100, 141, 594, 632, 835, 838, 1017
Sau3AI GATC 6 cut(s) 303, 328, 650, 754, 993, 1075
Sau96I GGNCC 1 cut(s) 588
ScaI AGTACT 1 cut(s) 547
SchI GAGTC 1 cut(s) 40
ScrFI CCNGG 4 cut(s) 185, 768, 1004, 1127
SduI GDGCHC 6 cut(s) 289, 302, 446, 564, 620, 1126
SetI ASST 9 cut(s) 322, 384, 446, 495, 617, 636, 693, 727, 1141
SfaNI GCATC 6 cut(s) 328, 821, 913, 962, 1003, 1139
SfcI CTRYAG 1 cut(s) 315
SfuI TTCGAA 1 cut(s) 470
SinI GGWCC 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 997
SmoI CTYRAG 1 cut(s) 997
Sse9I AATT 2 cut(s) 262, 393
SsiI CCGC 7 cut(s) 99, 141, 461, 586, 591, 776, 899
SspMI CTAG 2 cut(s) 173, 1134
SstI GAGCTC 1 cut(s) 446
StyD4I CCNGG 4 cut(s) 183, 766, 1002, 1125
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 3 cut(s) 566, 661, 670
TaqI TCGA 4 cut(s) 81, 192, 470, 907
TasI AATT 2 cut(s) 262, 393
TatI WGTACW 1 cut(s) 545
TauI GCSGC 2 cut(s) 102, 143
TfiI GAWTC 2 cut(s) 126, 472
Tru1I TTAA 2 cut(s) 216, 947
Tru9I TTAA 2 cut(s) 216, 947
TscAI CASTG 1 cut(s) 94
TseI GCWGC 5 cut(s) 593, 631, 834, 837, 1016
TspDTI ATGAA 3 cut(s) 255, 900, 909
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 588
XapI RAATTY 1 cut(s) 393
XbaI TCTAGA 1 cut(s) 1133
XmiI GTMKAC 1 cut(s) 192
XspI CTAG 2 cut(s) 173, 1134
ZrmI AGTACT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.