FvH4_7g18260
NAC Family

(NAC) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002549
Physical Location & Seq
Forward (+)
15284840 .. 15288075
3236 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g18260.t2

Sequence Viewer

Length: 1314 bp
ATGAGCTACGGTGGCTTCTCACTTCCAAAGGGTTTCCGGTTCCAGCCCTCCGATGACGAGCTGCTGAGTCACTACTTGCAGAAGAAGAATGACCGCGAGGACCCCGAGATCACGGCCATCATCCCTGAGATTGATGTGTCCAAGCACGAGCCTCGCGACTTGCCCGCACTGGTGTTCACAAGGGCCGATTTTCTGGACAGGGAGTGGTTCACGATGCCGGACTCTCCGGACATGGAGTGGTACTTCTTCAGTCCTAGAGTTTTTAAGCACAGCAACAGCAAAAGCAAAAGCACTAAGATCAATAGGACTACTGATGAAGGATATTGGAAGAAGCAAGGCAACGATCGCAGGATCACTGGGGCCTGTTCTGATAAGCAGATAGGGGCGAGGAGGATCTTGACCTTCTACCTTTCGAATAAACAGAAGACTGACTGGGTTATTCACGAGTTTTATCTCACTAAGGCCGATTCTGATGAGCAGATCGGCGACTTTGTTCTATGCCGCTTGAAGGACAACCGCTTGAAGAAGAAGTCGGGCAAGCCTGATCATGATCGGCAGGATGCTCCCTTGTGCGATGAAGATCAAGCATTCAGTGAGCTGGGAAATGCTGTTGTGATTCCCAAAGGCAATGATGATGAAGCTAAAGCTGAACATGGGGGTAGCTGTAGTATGGCCTCAAATGTTGAAAGTCCCTGTGGCAATGATGATAACGCTGAACTTGGTGGTGGTAGCCGCCACATCGTCCCTGTTATGCTTACTGAAGCAGAAGAATACCAAGGATTCAATGCACTACAAGATGCTGTTTTTGGATCTAATAGCAATGAGCAAGAATTTGGAGGCTCACAAACTGGCCCTTGTTATGTAGATAAGAATGATCATTCGGTCTCCATTGAAGGTCAACCTGATTCTGAGATGGTTAAAGAGCTACTTCACAAGCCAGTAGATCTGGCCTTACCCCATCCACCTCCGTCAACTCAACTGCAGTCACTAATATACACAAAGTCGGGCACCAACAATTCCCTTAATGATGAATATCATAAGAGGAAAAATCCATTTGGGGATAGTGACCCTTTTCTTACGAAGAAGAATCATACTGATGAAGTAGATAGCAACACTTCATCTAACTCTGAAAATCAACCTGCAGATCCGATTCCAAAGGGATGTTCTCAACCAGGAGGAAATCTGGAATCTGATTTGTTTAGGTCACAGAAACCACCATCAATAATCAGAGAACCGAGAGATCTTCCCCATGACAATGATTTCATTGAATGGGTTGATTTGCCATCTATAACAGAACTTATAGAAGGCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

438

Amino Acids

49.13

Weight (kDa)

4.93

Isoelectric Point (pI)

49.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 9 - 151 1.4e-29 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1147
AccB1I GGYRCC 1 cut(s) 1007
AccII CGCG 2 cut(s) 96, 156
AccIII TCCGGA 1 cut(s) 226
AciI CCGC 5 cut(s) 94, 165, 502, 517, 733
AclWI GGATC 4 cut(s) 359, 401, 817, 1139
AcoI YGGCCR 1 cut(s) 114
AcsI RAATTY 1 cut(s) 830
AcuI CTGAAG 2 cut(s) 232, 780
AfaI GTAC 1 cut(s) 242
AfiI CCNNNNNNNGG 1 cut(s) 508
AgsI TTSAA 6 cut(s) 508, 523, 686, 784, 893, 1268
AjnI CCWGG 1 cut(s) 1171
AjuI GAANNNNNNNTTGG 2 cut(s) 1147, 1179
AluBI AGCT 7 cut(s) 6, 61, 598, 641, 647, 663, 925
AluI AGCT 7 cut(s) 6, 61, 598, 641, 647, 663, 925
Alw26I GTCTC 1 cut(s) 889
AlwI GGATC 4 cut(s) 359, 401, 817, 1139
Ama87I CYCGRG 1 cut(s) 104
Aor13HI TCCGGA 1 cut(s) 226
AoxI GGCC 7 cut(s) 114, 183, 360, 462, 672, 850, 948
ApeKI GCWGC 1 cut(s) 61
ApoI RAATTY 1 cut(s) 830
AspS9I GGNCC 4 cut(s) 100, 183, 360, 851
AsuII TTCGAA 1 cut(s) 413
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 100
BaeGI GKGCMC 1 cut(s) 1010
BanI GGYRCC 1 cut(s) 1007
BauI CACGAG 2 cut(s) 146, 443
BbsI GAAGAC 1 cut(s) 431
BbvI GCAGC 1 cut(s) 48
BccI CCATC 5 cut(s) 125, 907, 966, 1225, 1291
BceAI ACGGC 1 cut(s) 129
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BciT130I CCWGG 1 cut(s) 1173
BclI TGATCA 2 cut(s) 544, 874
BcoDI GTCTC 1 cut(s) 889
BfaI CTAG 1 cut(s) 255
BfmI CTRYAG 3 cut(s) 664, 980, 1140
BfuAI ACCTGC 1 cut(s) 1147
BglII AGATCT 2 cut(s) 943, 1240
BisI GCNGC 3 cut(s) 62, 502, 733
BlsI GCNGC 3 cut(s) 63, 503, 734
Bme1390I CCNGG 1 cut(s) 1173
Bme18I GGWCC 1 cut(s) 100
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 4 cut(s) 100, 183, 360, 851
BmiI GGNNCC 4 cut(s) 41, 102, 361, 1009
BmrFI CCNGG 1 cut(s) 1173
BmrI ACTGGG 2 cut(s) 366, 442
BmsI GCATC 3 cut(s) 204, 550, 787
BmuI ACTGGG 2 cut(s) 366, 442
BpiI GAAGAC 1 cut(s) 431
Bpu14I TTCGAA 1 cut(s) 413
BsaBI GATNNNNATC 3 cut(s) 549, 579, 1032
BsaI GGTCTC 1 cut(s) 889
BsaJI CCNNGG 1 cut(s) 775
BsaWI WCCGGW 2 cut(s) 36, 226
Bsc4I CCNNNNNNNGG 1 cut(s) 508
Bse1I ACTGG 5 cut(s) 174, 361, 437, 853, 938
Bse3DI GCAATG 3 cut(s) 634, 706, 826
Bse8I GATNNNNATC 3 cut(s) 549, 579, 1032
BseAI TCCGGA 1 cut(s) 226
BseBI CCWGG 1 cut(s) 1173
BseDI CCNNGG 1 cut(s) 775
BseGI GGATG 4 cut(s) 120, 565, 958, 1166
BseJI GATNNNNATC 3 cut(s) 549, 579, 1032
BseLI CCNNNNNNNGG 1 cut(s) 508
BseMI GCAATG 3 cut(s) 634, 706, 826
BseMII CTCAG 3 cut(s) 56, 117, 900
BseNI ACTGG 5 cut(s) 174, 361, 437, 853, 938
BseRI GAGGAG 1 cut(s) 403
BseSI GKGCMC 1 cut(s) 1010
BseXI GCAGC 1 cut(s) 48
BseYI CCCAGC 1 cut(s) 598
Bsh1236I CGCG 2 cut(s) 96, 156
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 7 cut(s) 116, 185, 362, 464, 674, 852, 950
BshNI GGYRCC 1 cut(s) 1007
BsiEI CGRYCG 1 cut(s) 346
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 3 cut(s) 37, 218, 227
BslFI GGGAC 2 cut(s) 675, 728
BslI CCNNNNNNNGG 1 cut(s) 508
BsmAI GTCTC 1 cut(s) 889
BsmFI GGGAC 2 cut(s) 675, 728
BsmI GAATGC 1 cut(s) 587
BsnI GGCC 7 cut(s) 116, 185, 362, 464, 674, 852, 950
Bso31I GGTCTC 1 cut(s) 889
BsoBI CYCGRG 1 cut(s) 104
Bsp119I TTCGAA 1 cut(s) 413
Bsp1286I GDGCHC 1 cut(s) 1010
Bsp13I TCCGGA 1 cut(s) 226
Bsp68I TCGCGA 1 cut(s) 156
BspACI CCGC 5 cut(s) 94, 165, 502, 517, 733
BspANI GGCC 7 cut(s) 116, 185, 362, 464, 674, 852, 950
BspCNI CTCAG 3 cut(s) 57, 118, 901
BspEI TCCGGA 1 cut(s) 226
BspFNI CGCG 2 cut(s) 96, 156
BspHI TCATGA 1 cut(s) 547
BspLI GGNNCC 4 cut(s) 41, 102, 361, 1009
BspMAI CTGCAG 2 cut(s) 984, 1144
BspMI ACCTGC 1 cut(s) 1147
BspPI GGATC 4 cut(s) 359, 401, 817, 1139
BspT104I TTCGAA 1 cut(s) 413
BspT107I GGYRCC 1 cut(s) 1007
BspTNI GGTCTC 1 cut(s) 889
BsrDI GCAATG 3 cut(s) 634, 706, 826
BsrI ACTGG 5 cut(s) 174, 361, 437, 853, 938
BssECI CCNNGG 1 cut(s) 775
BssSI CACGAG 2 cut(s) 146, 443
BssT1I CCWWGG 1 cut(s) 775
Bst2BI CACGAG 2 cut(s) 146, 443
Bst2UI CCWGG 1 cut(s) 1173
Bst4CI ACNGT 1 cut(s) 11
BstBI TTCGAA 1 cut(s) 413
BstC8I GCNNGC 2 cut(s) 165, 539
BstDEI CTNAG 5 cut(s) 65, 126, 294, 459, 909
BstF5I GGATG 4 cut(s) 120, 565, 958, 1166
BstFNI CGCG 2 cut(s) 96, 156
BstMAI GTCTC 1 cut(s) 889
BstMCI CGRYCG 1 cut(s) 346
BstMWI GCNNNNNNNGC 2 cut(s) 12, 345
BstNI CCWGG 1 cut(s) 1173
BstSCI CCNGG 1 cut(s) 1171
BstSFI CTRYAG 3 cut(s) 664, 980, 1140
BstSLI GKGCMC 1 cut(s) 1010
BstUI CGCG 2 cut(s) 96, 156
BstV1I GCAGC 1 cut(s) 48
BstV2I GAAGAC 1 cut(s) 431
BstX2I RGATCY 5 cut(s) 393, 809, 943, 1144, 1240
BstYI RGATCY 5 cut(s) 393, 809, 943, 1144, 1240
BsuRI GGCC 7 cut(s) 116, 185, 362, 464, 674, 852, 950
BtgZI GCGATG 1 cut(s) 588
BtsCI GGATG 4 cut(s) 120, 565, 958, 1166
BtsIMutI CAGTG 3 cut(s) 167, 354, 598
BtuMI TCGCGA 1 cut(s) 156
BveI ACCTGC 1 cut(s) 1147
Cac8I GCNNGC 2 cut(s) 165, 539
CciI TCATGA 1 cut(s) 547
Cfr13I GGNCC 4 cut(s) 100, 183, 360, 851
Csp6I GTAC 1 cut(s) 241
CviAII CATG 4 cut(s) 232, 548, 653, 1250
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 5 cut(s) 65, 126, 294, 459, 909
EaeI YGGCCR 1 cut(s) 114
Eco130I CCWWGG 1 cut(s) 775
Eco31I GGTCTC 1 cut(s) 889
Eco47I GGWCC 1 cut(s) 100
Eco57I CTGAAG 2 cut(s) 232, 780
Eco88I CYCGRG 1 cut(s) 104
EcoO109I RGGNCCY 2 cut(s) 100, 360
EcoRII CCWGG 1 cut(s) 1171
EcoT14I CCWWGG 1 cut(s) 775
ErhI CCWWGG 1 cut(s) 775
FaeI CATG 4 cut(s) 235, 551, 656, 1253
FalI AAGNNNNNCTT 2 cut(s) 912, 944
FaqI GGGAC 2 cut(s) 675, 728
FatI CATG 4 cut(s) 231, 547, 652, 1249
FauI CCCGC 1 cut(s) 172
FbaI TGATCA 2 cut(s) 544, 874
Fnu4HI GCNGC 3 cut(s) 62, 502, 733
FokI GGATG 4 cut(s) 107, 572, 945, 1173
Fsp4HI GCNGC 3 cut(s) 62, 502, 733
FspBI CTAG 1 cut(s) 255
GluI GCNGC 3 cut(s) 62, 502, 733
GsaI CCCAGC 1 cut(s) 602
HaeIII GGCC 7 cut(s) 116, 185, 362, 464, 674, 852, 950
HapII CCGG 3 cut(s) 37, 218, 227
Hin1II CATG 4 cut(s) 235, 551, 656, 1253
HincII GTYRAC 2 cut(s) 899, 972
HindII GTYRAC 2 cut(s) 899, 972
HinfI GANTC 9 cut(s) 67, 221, 467, 616, 780, 905, 1087, 1150, 1187
HpaII CCGG 3 cut(s) 37, 218, 227
Hpy166II GTNNAC 4 cut(s) 177, 210, 899, 972
Hpy188I TCNGA 8 cut(s) 52, 370, 472, 910, 1129, 1149, 1192, 1229
Hpy188III TCNNGA 8 cut(s) 155, 194, 211, 227, 397, 443, 548, 1184
Hpy8I GTNNAC 4 cut(s) 177, 210, 899, 972
HpyAV CCTTC 5 cut(s) 311, 412, 502, 887, 1298
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4V TGCA 4 cut(s) 79, 788, 982, 1142
HpyF10VI GCNNNNNNNGC 2 cut(s) 12, 345
HpyF3I CTNAG 5 cut(s) 65, 126, 294, 459, 909
Hsp92II CATG 4 cut(s) 235, 551, 656, 1253
Kpn2I TCCGGA 1 cut(s) 226
Ksp22I TGATCA 2 cut(s) 544, 874
LmnI GCTCC 1 cut(s) 568
Lsp1109I GCAGC 1 cut(s) 48
LweI GCATC 3 cut(s) 204, 550, 787
MaeI CTAG 1 cut(s) 255
MaeIII GTNAC 4 cut(s) 68, 984, 1064, 1203
MflI RGATCY 5 cut(s) 393, 809, 943, 1144, 1240
MhlI GDGCHC 1 cut(s) 1010
MluCI AATT 2 cut(s) 830, 1015
MlyI GAGTC 2 cut(s) 76, 215
MroI TCCGGA 1 cut(s) 226
MseI TTAA 3 cut(s) 264, 918, 1023
MslI CAYNNNNRTG 2 cut(s) 1095, 1254
MspI CCGG 3 cut(s) 37, 218, 227
MspR9I CCNGG 1 cut(s) 1173
Mva1269I GAATGC 1 cut(s) 587
MvaI CCWGG 1 cut(s) 1173
MvnI CGCG 2 cut(s) 96, 156
MwoI GCNNNNNNNGC 2 cut(s) 12, 345
NlaIII CATG 4 cut(s) 235, 551, 656, 1253
NlaIV GGNNCC 4 cut(s) 41, 102, 361, 1009
NmuCI GTSAC 4 cut(s) 68, 984, 1064, 1203
NruI TCGCGA 1 cut(s) 156
NspV TTCGAA 1 cut(s) 413
PagI TCATGA 1 cut(s) 547
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PctI GAATGC 1 cut(s) 587
PfeI GAWTC 7 cut(s) 467, 616, 780, 905, 1087, 1150, 1187
PkrI GCNGC 3 cut(s) 63, 503, 734
Ple19I CGATCG 1 cut(s) 346
PleI GAGTC 2 cut(s) 75, 215
PpsI GAGTC 2 cut(s) 75, 215
PpuMI RGGWCCY 1 cut(s) 100
Psp5II RGGWCCY 1 cut(s) 100
Psp6I CCWGG 1 cut(s) 1171
PspFI CCCAGC 1 cut(s) 598
PspGI CCWGG 1 cut(s) 1171
PspN4I GGNNCC 4 cut(s) 41, 102, 361, 1009
PspPI GGNCC 4 cut(s) 100, 183, 360, 851
PspPPI RGGWCCY 1 cut(s) 100
PstI CTGCAG 2 cut(s) 984, 1144
PsuI RGATCY 5 cut(s) 393, 809, 943, 1144, 1240
PvuI CGATCG 1 cut(s) 346
RruI TCGCGA 1 cut(s) 156
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
RseI CAYNNNNRTG 2 cut(s) 1095, 1254
SaqAI TTAA 3 cut(s) 264, 918, 1023
SatI GCNGC 3 cut(s) 62, 502, 733
Sau96I GGNCC 4 cut(s) 100, 183, 360, 851
SchI GAGTC 2 cut(s) 76, 215
ScrFI CCNGG 1 cut(s) 1173
SduI GDGCHC 1 cut(s) 1010
SfaNI GCATC 3 cut(s) 204, 550, 787
SfcI CTRYAG 3 cut(s) 664, 980, 1140
SfuI TTCGAA 1 cut(s) 413
SinI GGWCC 1 cut(s) 100
SmiMI CAYNNNNRTG 2 cut(s) 1095, 1254
Sse9I AATT 2 cut(s) 830, 1015
SsiI CCGC 5 cut(s) 94, 165, 502, 517, 733
SspMI CTAG 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 1171
StyI CCWWGG 1 cut(s) 775
TaaI ACNGT 1 cut(s) 11
TaqI TCGA 1 cut(s) 413
TaqII GACCGA 1 cut(s) 871
TasI AATT 2 cut(s) 830, 1015
TauI GCSGC 2 cut(s) 504, 735
TfiI GAWTC 7 cut(s) 467, 616, 780, 905, 1087, 1150, 1187
Tru1I TTAA 3 cut(s) 264, 918, 1023
Tru9I TTAA 3 cut(s) 264, 918, 1023
TscAI CASTG 3 cut(s) 174, 361, 598
TseFI GTSAC 4 cut(s) 68, 984, 1064, 1203
TseI GCWGC 1 cut(s) 61
Tsp45I GTSAC 4 cut(s) 68, 984, 1064, 1203
TspDTI ATGAA 7 cut(s) 330, 591, 651, 1044, 1107, 1113, 1252
TspGWI ACGGA 1 cut(s) 957
TspRI CASTG 3 cut(s) 174, 361, 598
VpaK11BI GGWCC 1 cut(s) 100
XapI RAATTY 1 cut(s) 830
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.