Rroxscaffold_4G00294990
NAC Family

(NAC) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002549
Physical Location & Seq
Forward (+)
14887516 .. 14891985
4470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00294990.1

Sequence Viewer

Length: 2157 bp
ATGATGAGCAGCGCCTGCATCACACTTCCGAAGGGTCTCCGATTCCAGCCCTCCGACGAGGACCTGCTGAGTGATTACTTGGACAAGAAGAATCAGCGCAAGGATCTCGAAATCAAGGCCATCATCTCTGAAATCAAGGTGTTGGAGCACAAGCCTGGCGACTTAGCAGCCCTGGTACCAATGGTGAAATTTCTGGACAGGGAGTGGGAATTCACCATGGCAGACTCTCCGGACATGGAGCGGTACTTCTACAGTCCGCGAGTTTTTAAACACAGCAAAAGCAAAAGCACTCGGTCCAACAGGACCACGGAGGAAGGCTACTGGAAGAAGCAAGGCAAGGACCGCAGAATCACTCGGGCACGTTCTGATGAGCAGATCGGGGGGAAGAGGATCTTGACCTTCTACCTGCCGGACAAACAGAAGACTGACTGGGTTATCCATGAGTATTATGTCACTAAGCCGAATTCTGATGAGCAGATCGGGGACTTTGTTCTCTGCCGCTTGAAGAACAAGTCAGGCAAGTCTGATCATGATCAGCAGGATGATCCACTGTGCGATGGAGGTGAACCTAATAGTGGTAGCTGCAGTATGGTCTCCAATGTTGAAGATCAAGGATCCACAAAGCTGGGAACTGTTCTTCCGATTCCCAATGGCAATAATGATGAAGCTGAATATGGTGGCGGTAGCTGCAGTATGGCCTTTGATGTTGAAAATCAAGCATCCAAACAGCTGGAAAATGATCTTCCGATTCCCAATGGCAATGATAATGAAGCTGAACATGGAAGTAGCTGCCTCATCACCCCTGGTTTGGAAAATGAAGCTGCAGCATTTGGTACATTCAAGGAGCTGATAGATGCTGTTTGGGAATATGATGACAAGGAACAAGAAGTTAGAGGCTCGCAAACTGATCCATGTTATATTGATAAGAATGATCTTTCGATCTATGTCAAAGGTCAACCAGCTGATCGCTGCAATTCATCTGATTCTGATATGCTACTTGATGAGCCAGGACATCTGGCCTTACCCCTTTATCCACCTCCGCCACCCAATATGTTTGAAAAGCCACTTGATGAGCCAGAAAATCTGGCCTTACCCCTGACCAATGTTGAAGATCAAGGTTCCAAAGAGCTGGCAACTGTTCTTCCGATTCCCAATGGCAATAATGATGAAGCTGAACATGGTGGCGGTAGCTGCAGTATGGCCTTTGATGTTGAAAATCAAGCATCCAAACAGCTGGAAAATGATCTTCTGATTCGCAATGGTAATGATAATGAAGCTGAACATGGAAGTAGCTGCCTCATCACCCCTGGTTTGGAAAATGAAGCTGCAGCATTTGGTATGCTTACAGAAGAAGAAATAAACCAGACATTCAAGGAGCTGATAGATGCTGTTTGGGAATATGATGACAAGGAACAAGAAGTTAGAGGCTCGCAAACTGATCCATGTTATATTGATAAGAATGATCTTTCGATCTATGTCAAAGGTCAACCAGCTGATCGCTGCAATTCATCTGATTCTGATATGGTTGAAGAGCTACTTGATGAGCCAGGACATCTGGCCTTACCCCTTCATCCACCTCCGCCACCCAATATGTTTGAAAAGCCACTTGATGAGCCAGGAAATCTGGCCTTACCCCTTCATCCACCTCCATCACCTCAACTGCAGTCACCAATATACACAAAGCTGGGCAACACCAATGTCCAAAATGATGAATCTCGTAAGAGGAAATATTCATTTGGGGATAATGACCCTTTTCTTACGAAGAAGAATCATATTGATGAAGTAGATAGCAACTCTTCATCTAATTCTGAAAATCAAGCTGCGGATGCGATTCCAGAGGGATATTCTCAACCAGGAGAAAGTCTGGAATCTGATTCATTTCAGTCGCAGGAACTACCATCAATAAACAGAGAACCGGGAGATTTTCCCCATGACAATAATAGTGTTATTGAATGGGATGATTGGCCACCTATAACAGAAGGTTTTGACTCTTCTCTCGAGTTCTCAGATACATTTATTGATTGCTACCTCTGTGACGGAAACACCGAAGTCATCTCTGTATCGGTAAACGATTATTCTACATTAGCCATAGGAGAAACACCCAATGTAGCTAATCAACCTTTATTTATAGGAGAATATCAGTGGAGGCCACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

718

Amino Acids

80.01

Weight (kDa)

4.35

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 10 - 150 1.2e-25 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 72, 414
Acc65I GGTACC 1 cut(s) 175
AccB1I GGYRCC 1 cut(s) 175
AccBSI CCGCTC 1 cut(s) 241
AccII CGCG 1 cut(s) 259
AccIII TCCGGA 1 cut(s) 229
AciI CCGC 9 cut(s) 241, 257, 343, 499, 681, 1040, 1185, 1580, 1823
AclWI GGATC 7 cut(s) 111, 398, 539, 609, 622, 902, 1433
AcoI YGGCCR 1 cut(s) 1964
AcsI RAATTY 3 cut(s) 188, 209, 463
AfaI GTAC 3 cut(s) 177, 245, 835
AfiI CCNNNNNNNGG 3 cut(s) 575, 808, 1312
AjnI CCWGG 8 cut(s) 154, 171, 802, 1006, 1306, 1546, 1615, 1852
AloI GAACNNNNNNTCC 2 cut(s) 622, 654
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 2 cut(s) 41, 598
AlwI GGATC 7 cut(s) 111, 398, 539, 609, 622, 902, 1433
AlwNI CAGNNNCTG 1 cut(s) 15
Ama87I CYCGRG 2 cut(s) 354, 1997
Aor13HI TCCGGA 1 cut(s) 229
AoxI GGCC 9 cut(s) 117, 696, 1017, 1086, 1200, 1557, 1626, 1964, 2147
ApoI RAATTY 3 cut(s) 188, 209, 463
Asp718I GGTACC 1 cut(s) 175
AspLEI GCGC 2 cut(s) 14, 99
AspS9I GGNCC 4 cut(s) 61, 294, 303, 340
AsuC2I CCSGG 1 cut(s) 1917
AsuHPI GGTGA 7 cut(s) 196, 205, 575, 790, 1294, 1644, 1659
AvaI CYCGRG 2 cut(s) 354, 1997
AvaII GGWCC 4 cut(s) 61, 294, 303, 340
BaeGI GKGCMC 1 cut(s) 361
BalI TGGCCA 1 cut(s) 1966
BamHI GGATCC 1 cut(s) 614
BanI GGYRCC 1 cut(s) 175
BbsI GAAGAC 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 150
BccI CCATC 4 cut(s) 128, 551, 1657, 1906
BcgI CGANNNNNNTGC 2 cut(s) 88, 122
BciT130I CCWGG 8 cut(s) 156, 173, 804, 1008, 1308, 1548, 1617, 1854
BclI TGATCA 2 cut(s) 526, 532
BcnI CCSGG 1 cut(s) 1917
BcoDI GTCTC 2 cut(s) 41, 598
BfmI CTRYAG 7 cut(s) 250, 583, 688, 822, 1192, 1326, 1661
BfoI RGCGCY 1 cut(s) 15
BfuAI ACCTGC 2 cut(s) 72, 414
Bme1390I CCNGG 9 cut(s) 156, 173, 804, 1008, 1308, 1548, 1617, 1854, 1917
Bme18I GGWCC 4 cut(s) 61, 294, 303, 340
BmeT110I CYCGRG 2 cut(s) 354, 1997
BmgT120I GGNCC 4 cut(s) 61, 294, 303, 340
BmiI GGNNCC 3 cut(s) 177, 616, 1120
BmrFI CCNGG 9 cut(s) 156, 173, 804, 1008, 1308, 1548, 1617, 1854, 1917
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 6 cut(s) 27, 728, 844, 1232, 1375, 1816
BmuI ACTGGG 1 cut(s) 439
BpiI GAAGAC 1 cut(s) 428
BpuMI CCSGG 1 cut(s) 1917
BsaBI GATNNNNATC 1 cut(s) 531
BsaI GGTCTC 2 cut(s) 41, 598
BsaJI CCNNGG 5 cut(s) 171, 216, 306, 802, 1306
BsaWI WCCGGW 1 cut(s) 229
Bsc4I CCNNNNNNNGG 3 cut(s) 575, 808, 1312
Bse1I ACTGG 2 cut(s) 326, 434
Bse3DI GCAATG 2 cut(s) 766, 1264
Bse8I GATNNNNATC 1 cut(s) 531
BseAI TCCGGA 1 cut(s) 229
BseBI CCWGG 8 cut(s) 156, 173, 804, 1008, 1308, 1548, 1617, 1854
BseDI CCNNGG 5 cut(s) 171, 216, 306, 802, 1306
BseGI GGATG 7 cut(s) 547, 719, 1223, 1570, 1639, 1831, 1963
BseJI GATNNNNATC 1 cut(s) 531
BseLI CCNNNNNNNGG 3 cut(s) 575, 808, 1312
BseMI GCAATG 2 cut(s) 766, 1264
BseMII CTCAG 2 cut(s) 59, 2019
BseNI ACTGG 2 cut(s) 326, 434
BseSI GKGCMC 1 cut(s) 361
BseYI CCCAGC 2 cut(s) 625, 1684
Bsh1236I CGCG 1 cut(s) 259
BshFI GGCC 9 cut(s) 119, 698, 1019, 1088, 1202, 1559, 1628, 1966, 2149
BshNI GGYRCC 1 cut(s) 175
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 2 cut(s) 354, 1997
BsiSI CCGG 3 cut(s) 230, 410, 1916
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 3 cut(s) 575, 808, 1312
BsmAI GTCTC 2 cut(s) 41, 598
BsmFI GGGAC 1 cut(s) 497
BsnI GGCC 9 cut(s) 119, 698, 1019, 1088, 1202, 1559, 1628, 1966, 2149
Bso31I GGTCTC 2 cut(s) 41, 598
BsoBI CYCGRG 2 cut(s) 354, 1997
Bsp1286I GDGCHC 2 cut(s) 150, 361
Bsp13I TCCGGA 1 cut(s) 229
Bsp19I CCATGG 1 cut(s) 216
BspACI CCGC 9 cut(s) 241, 257, 343, 499, 681, 1040, 1185, 1580, 1823
BspANI GGCC 9 cut(s) 119, 698, 1019, 1088, 1202, 1559, 1628, 1966, 2149
BspCNI CTCAG 2 cut(s) 60, 2018
BspEI TCCGGA 1 cut(s) 229
BspFNI CGCG 1 cut(s) 259
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 3 cut(s) 177, 616, 1120
BspMAI CTGCAG 6 cut(s) 587, 692, 826, 1196, 1330, 1665
BspMI ACCTGC 2 cut(s) 72, 414
BspPI GGATC 7 cut(s) 111, 398, 539, 609, 622, 902, 1433
BspQI GCTCTTC 1 cut(s) 1524
BspT107I GGYRCC 1 cut(s) 175
BspTNI GGTCTC 2 cut(s) 41, 598
BsrBI CCGCTC 1 cut(s) 241
BsrDI GCAATG 2 cut(s) 766, 1264
BsrI ACTGG 2 cut(s) 326, 434
BssECI CCNNGG 5 cut(s) 171, 216, 306, 802, 1306
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 8 cut(s) 156, 173, 804, 1008, 1308, 1548, 1617, 1854
Bst4CI ACNGT 5 cut(s) 254, 552, 634, 1138, 2154
Bst6I CTCTTC 4 cut(s) 380, 1524, 1801, 1996
BstAPI GCANNNNNTGC 1 cut(s) 15
BstC8I GCNNGC 4 cut(s) 16, 899, 1131, 1430
BstDEI CTNAG 4 cut(s) 68, 163, 456, 2005
BstDSI CCRYGG 2 cut(s) 216, 306
BstF5I GGATG 7 cut(s) 547, 719, 1223, 1570, 1639, 1831, 1963
BstFNI CGCG 1 cut(s) 259
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 2 cut(s) 14, 99
BstMAI GTCTC 2 cut(s) 41, 598
BstMWI GCNNNNNNNGC 5 cut(s) 15, 342, 687, 1191, 1826
BstNI CCWGG 8 cut(s) 156, 173, 804, 1008, 1308, 1548, 1617, 1854
BstSCI CCNGG 9 cut(s) 154, 171, 802, 1006, 1306, 1546, 1615, 1852, 1915
BstSFI CTRYAG 7 cut(s) 250, 583, 688, 822, 1192, 1326, 1661
BstSLI GKGCMC 1 cut(s) 361
BstUI CGCG 1 cut(s) 259
BstV2I GAAGAC 1 cut(s) 428
BstX2I RGATCY 3 cut(s) 103, 390, 614
BstXI CCANNNNNNTGG 4 cut(s) 625, 730, 1129, 1234
BstYI RGATCY 3 cut(s) 103, 390, 614
BsuRI GGCC 9 cut(s) 119, 698, 1019, 1088, 1202, 1559, 1628, 1966, 2149
BtgI CCRYGG 2 cut(s) 216, 306
BtgZI GCGATG 1 cut(s) 570
BtsCI GGATG 7 cut(s) 547, 719, 1223, 1570, 1639, 1831, 1963
BtsIMutI CAGTG 2 cut(s) 548, 2147
BveI ACCTGC 2 cut(s) 72, 414
Cac8I GCNNGC 4 cut(s) 16, 899, 1131, 1430
CaiI CAGNNNCTG 1 cut(s) 15
CciI TCATGA 1 cut(s) 529
CfoI GCGC 2 cut(s) 14, 99
Cfr13I GGNCC 4 cut(s) 61, 294, 303, 340
Csp6I GTAC 3 cut(s) 176, 244, 834
CviQI GTAC 3 cut(s) 176, 244, 834
DdeI CTNAG 4 cut(s) 68, 163, 456, 2005
DraI TTTAAA 1 cut(s) 268
EaeI YGGCCR 1 cut(s) 1964
Eam1104I CTCTTC 4 cut(s) 380, 1524, 1801, 1996
EarI CTCTTC 4 cut(s) 380, 1524, 1801, 1996
EciI GGCGGA 2 cut(s) 1029, 1569
Eco130I CCWWGG 1 cut(s) 216
Eco31I GGTCTC 2 cut(s) 41, 598
Eco47I GGWCC 4 cut(s) 61, 294, 303, 340
Eco88I CYCGRG 2 cut(s) 354, 1997
EcoO109I RGGNCCY 1 cut(s) 61
EcoRI GAATTC 2 cut(s) 209, 463
EcoRII CCWGG 8 cut(s) 154, 171, 802, 1006, 1306, 1546, 1615, 1852
EcoT14I CCWWGG 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 216
FalI AAGNNNNNCTT 4 cut(s) 377, 409, 1521, 1553
FaqI GGGAC 1 cut(s) 497
FbaI TGATCA 2 cut(s) 526, 532
FokI GGATG 7 cut(s) 554, 706, 1210, 1557, 1626, 1838, 1970
GlaI GCGC 2 cut(s) 13, 98
GsaI CCCAGC 2 cut(s) 629, 1688
HaeII RGCGCY 1 cut(s) 15
HaeIII GGCC 9 cut(s) 119, 698, 1019, 1088, 1202, 1559, 1628, 1966, 2149
HapII CCGG 3 cut(s) 230, 410, 1916
HhaI GCGC 2 cut(s) 14, 99
Hin6I GCGC 2 cut(s) 12, 97
HinP1I GCGC 2 cut(s) 12, 97
HincII GTYRAC 2 cut(s) 956, 1487
HindII GTYRAC 2 cut(s) 956, 1487
HpaII CCGG 3 cut(s) 230, 410, 1916
HphI GGTGA 7 cut(s) 196, 205, 575, 790, 1294, 1644, 1659
Hpy166II GTNNAC 4 cut(s) 566, 956, 1487, 2068
Hpy188III TCNNGA 8 cut(s) 107, 194, 230, 394, 530, 1835, 1865, 1997
Hpy8I GTNNAC 4 cut(s) 566, 956, 1487, 2068
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 6 cut(s) 25, 308, 409, 1577, 1646, 1973
HpyCH4III ACNGT 5 cut(s) 254, 552, 634, 1138, 2154
HpyCH4IV ACGT 1 cut(s) 361
HpyCH4V TGCA 9 cut(s) 18, 585, 690, 824, 972, 1194, 1328, 1503, 1663
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 342, 687, 1191, 1826
HpyF3I CTNAG 4 cut(s) 68, 163, 456, 2005
HpySE526I ACGT 1 cut(s) 361
HspAI GCGC 2 cut(s) 12, 97
Kpn2I TCCGGA 1 cut(s) 229
KpnI GGTACC 1 cut(s) 179
Ksp22I TGATCA 2 cut(s) 526, 532
LguI GCTCTTC 1 cut(s) 1524
LmnI GCTCC 4 cut(s) 145, 238, 844, 1375
LweI GCATC 6 cut(s) 27, 728, 844, 1232, 1375, 1816
MaeII ACGT 1 cut(s) 361
MaeIII GTNAC 3 cut(s) 451, 1665, 2033
MbiI CCGCTC 1 cut(s) 241
MflI RGATCY 3 cut(s) 103, 390, 614
MhlI GDGCHC 2 cut(s) 150, 361
MlsI TGGCCA 1 cut(s) 1966
MluCI AATT 6 cut(s) 188, 209, 463, 973, 1504, 1804
MluNI TGGCCA 1 cut(s) 1966
MlyI GAGTC 2 cut(s) 218, 1982
MmeI TCCRAC 3 cut(s) 78, 123, 321
Mox20I TGGCCA 1 cut(s) 1966
MroI TCCGGA 1 cut(s) 229
MscI TGGCCA 1 cut(s) 1966
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 1 cut(s) 1776
Msp20I TGGCCA 1 cut(s) 1966
MspA1I CMGCKG 4 cut(s) 730, 962, 1234, 1493
MspI CCGG 3 cut(s) 230, 410, 1916
MspR9I CCNGG 9 cut(s) 156, 173, 804, 1008, 1308, 1548, 1617, 1854, 1917
MvaI CCWGG 8 cut(s) 156, 173, 804, 1008, 1308, 1548, 1617, 1854
MvnI CGCG 1 cut(s) 259
MwoI GCNNNNNNNGC 5 cut(s) 15, 342, 687, 1191, 1826
NciI CCSGG 1 cut(s) 1917
NcoI CCATGG 1 cut(s) 216
NlaIV GGNNCC 3 cut(s) 177, 616, 1120
NmuCI GTSAC 3 cut(s) 451, 1665, 2033
PaeR7I CTCGAG 1 cut(s) 1997
PagI TCATGA 1 cut(s) 529
PciSI GCTCTTC 1 cut(s) 1524
PcsI WCGNNNNNNNCGW 1 cut(s) 2043
PleI GAGTC 2 cut(s) 218, 1982
PpsI GAGTC 2 cut(s) 218, 1982
PpuMI RGGWCCY 1 cut(s) 61
Psp5II RGGWCCY 1 cut(s) 61
Psp6I CCWGG 8 cut(s) 154, 171, 802, 1006, 1306, 1546, 1615, 1852
PspFI CCCAGC 2 cut(s) 625, 1684
PspGI CCWGG 8 cut(s) 154, 171, 802, 1006, 1306, 1546, 1615, 1852
PspN4I GGNNCC 3 cut(s) 177, 616, 1120
PspPI GGNCC 4 cut(s) 61, 294, 303, 340
PspPPI RGGWCCY 1 cut(s) 61
PstI CTGCAG 6 cut(s) 587, 692, 826, 1196, 1330, 1665
PstNI CAGNNNCTG 1 cut(s) 15
PsuI RGATCY 3 cut(s) 103, 390, 614
PvuII CAGCTG 4 cut(s) 730, 962, 1234, 1493
RsaI GTAC 3 cut(s) 177, 245, 835
RsaNI GTAC 3 cut(s) 176, 244, 834
RseI CAYNNNNRTG 1 cut(s) 1776
SapI GCTCTTC 1 cut(s) 1524
SaqAI TTAA 1 cut(s) 267
Sau96I GGNCC 4 cut(s) 61, 294, 303, 340
SchI GAGTC 2 cut(s) 218, 1982
ScrFI CCNGG 9 cut(s) 156, 173, 804, 1008, 1308, 1548, 1617, 1854, 1917
SduI GDGCHC 2 cut(s) 150, 361
SfaNI GCATC 6 cut(s) 27, 728, 844, 1232, 1375, 1816
SfcI CTRYAG 7 cut(s) 250, 583, 688, 822, 1192, 1326, 1661
Sfr274I CTCGAG 1 cut(s) 1997
SinI GGWCC 4 cut(s) 61, 294, 303, 340
SlaI CTCGAG 1 cut(s) 1997
SmiMI CAYNNNNRTG 1 cut(s) 1776
SmlI CTYRAG 1 cut(s) 1997
SmoI CTYRAG 1 cut(s) 1997
Sse9I AATT 6 cut(s) 188, 209, 463, 973, 1504, 1804
SsiI CCGC 9 cut(s) 241, 257, 343, 499, 681, 1040, 1185, 1580, 1823
SspI AATATT 1 cut(s) 1730
StyD4I CCNGG 9 cut(s) 154, 171, 802, 1006, 1306, 1546, 1615, 1852, 1915
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 5 cut(s) 254, 552, 634, 1138, 2154
TaiI ACGT 1 cut(s) 364
TaqI TCGA 4 cut(s) 108, 938, 1469, 1998
TaqII GACCGA 1 cut(s) 282
TasI AATT 6 cut(s) 188, 209, 463, 973, 1504, 1804
TauI GCSGC 1 cut(s) 501
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 2 cut(s) 555, 2147
TseFI GTSAC 3 cut(s) 451, 1665, 2033
Tsp45I GTSAC 3 cut(s) 451, 1665, 2033
TspGWI ACGGA 2 cut(s) 323, 2052
TspRI CASTG 2 cut(s) 555, 2147
VpaK11BI GGWCC 4 cut(s) 61, 294, 303, 340
XapI RAATTY 3 cut(s) 188, 209, 463
XhoI CTCGAG 1 cut(s) 1997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.