Rorug01G0289900
NAC Family

(NAC) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002549
Physical Location & Seq
Forward (+)
40547539 .. 40552287
4749 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0289900.1

Sequence Viewer

Length: 3651 bp
ATGGATTGTCTTTGTGCAATTGGTAACGGGGTTGTTGGAAAAATAGCCGAGTACACGGTTGAACCCTTTGGACGTCTAGTGGGACGCCAAGTGGGTTATCTAATCCACTACAACAAGAATCTTCAAACTCTAATCACTCAAGTGAAGAATTTGAGCGTCGCCAGAGATAGAGTAAAGCAACAGGTTGATGAAGCTGATGGAAGAGGTGAGAAAATCCATAATGATGTCCAAAAATGGCTCGAAGAAGTGGCTCAGATCATGAAAGACACAGAAGAATTCTTGAAGGGAAAATGCCAAGCAACTGGAGAGCCAAACCAAGTCTTGAAGGATGAAGGCCAAGCAAATGAAGAGCCAAACGAAGTCTTGAAAGATGAACGTCAAGGAAAGCTGAAGCGTCTCCATCAATTTTGTCCTAATCCGAGACTTCGTTATCAACTAAGCAGAAAGTCAACAAAGTTGGTGGAGGCTGTGGATGAACTCTACAACAGAAAAGACTTCTCTAGTGTTTCATACAGTGTTCGCCCACAAGAGGTATGTGTCATATCTACTGAAGATTATGAGGCATTTGATTCAAGGATCTCAACTTTGAATAAAATCATGGATGAATTGAGACGTCCCAGTGCCGACATCATTCTAGTGTATGGTATTGGGGGTGTGGGTAAGACCACACTAGTGGAAGAAGTTCTCAGGCAAGTTACGAAAGATAAGTTGTTTGATGATGCAGTCATGGTACGAGATGTGAAAAATCCAAACTTGGAAGCAATTCAAGAAGAAATTGCTGAAAAGTTAAGTCTTGAAGTTTTGTCAAACCAGACTATCTCCGTAAGAGCCAATAAAATATGTGAAAGAATAAAAAACAAGAAGACTCTTGTGATTTTGGATGATGTCTGGGAACAAATTAACTTGAAAACTCTCGGGCTTCCCCACATACCTACTTGTAAAATACTGCTGACCTCTAGAACTGGAAAGGTATTAACTGCTGACTACATTAAGCAGAGGGAGTTTCTGCTCGGCATTTTAGGCCAAGATGAAACTTGGCTTTTATTTGAGAAGACAGCAGGGGATGTTACTAAAGATTCCGCTATAAAAACCTTAGCAACTCAAGTAGCCCAAAGATGTGGAGGTTTACCTCTTTTGATTATCGCAGTAGCAAGAGCTTTAGAACATAGTTCTTTACCTATATGGGAATATGCCCTGACAAGTCTTCACAGGTTTGATGAGAAAGAAGACTTCACGGAACAAGCTTACTTGGGTCTTGAATGGAGTTACACTCGATTGAATGAGCAGCTTAAGCCATTGTTCTTGTTGTGTGGTACTACTATACAGGGGAACTCTATTTCTTTGACGAACTTATTGAAATATAGTATGGGCTTGGGTTTTTTTAAAGGTCTTTCAGTTGAAAAAGCTCGAAATGCATTGTACACACAAGTTGATAAACTTAAGAGTTCTTGTCTATTGCTAGACGGAGATGATAATACGTGTGTCAGAATGCATGATCTTTTACATGATGTTGCTATCCGGATTGCGACTAGAGATCAAGACGTCCTAGTACAAAATGGAGTTCATCAGTTGGAAGAATGGCCAGATCAAGAATCGTTTAAAAGGTGCAGTAAGATCTCCTGGTCCTGTTGCAATATCCCCAGTCTTCCTGAAATACCTTGGAAATGTCCTGAGCTAGAAATGTTTCGTTTGCATGGTAATGGTGAAAAAAATGATCACCATTCGAAAATTCCAAGCAATTTTTTTGAAGAAATGCCAAAACTCAAGGTGTTGGATATTTGCAATCTGCCTCTTATGTCACTACCTCCATCTCTTGAATTGCTGAAAACCAATCTTCAAACATTGTGTTTAGATGGATGCACGTTGGAAGACATAGCTCGAGTCGGAGAGCTAACCAACTTGGAAACTCTTAGCTTTGCTAACTCCAAGGTCAAAGAGTTGCCGAAAGAAATAGCGAAATTGACTCATCTGCGGTTGTTGGACTTGTCGGATTGCCCTGAGCTTGAAGTGATTTCACCTAATGTCATATCAAGTTTGACAAGGCTCGAAGACTTGAGAATGAAAAACAGTTTTGAAAAATGGGAGGCTGAGGGAGACATCAACGAAATAAAGAATGCTAGTCTTTTGGAGCTGAAGCAGTTATCTGGACTAACTGCATTAGAAATATATATTCCAGATGTTGATATTCTTCCACCAAACTTGTTCTCCAATATGCTGAAAAGATATCATATATATATTGGTGGTGTGTGGAAAAGCTGGTTGCCGTGGGAGAAAACTATGGCTGAAACGACCCTCAATACACTAAAGCTCGAGCTCACTCCCAGCAATCAATCGGATCAAGGGCTAAGACAGCTGATGATGAGATCTGAAGATTTGGCCTTGGATGTGATGGAAGGTGTTAATAATAGTTTGTATCAATTAGACAACGAAGGTTCTCTGCACCTGAAACGTCTCCAACTCCAAAAGAATGTTGATCTTACATATATCATTAACAAGAAGGTTGTGTTCCCCAACTTAACAACCTTGAGCATTCAAAATTGTGATCATTTGAGATTCTTGTTCTCATGTTCTATGGCTAAAAGTTTCTTACAGCTCAAACATCTTGAGATATCCGACTGCAACAGCATGGAAGAGGTAGTATCATCAACAACAGATTACAATGAAGAAAATATGAATAACATGTTCTGCAAGCTTGAAACTCTGAAACTGGAACGACTTCCATGCCTCGGTAGAATCTGCACAGGTACTCGTATTGAATTTCCGTCCTTGAAGAAGTTGGTTATATGCTTTTGTGAAAAATTGAAGGCATTCATCTGTGACCCTATGAGTGATAAAAGTACAAGAATCTGCAAAGAAATTGAAGAGAGGGAGTCAGAGGAGAGGCCAAGTGTTGAACATGAGAGTGTTGCAGAACGCTGCCTTTTTAACGACAAGGTAAGATTTCCTAGCTTGGAGGAATTGAGAATTTGGTCATTAGACGAGTTGCAGACAATATGGCACAATAACCAACTTGCTGATCAAGACTCTTTTTGCCGACTCAAACTAGTTGACGTTAGTTATTGCAAGAGTCTGATAAGTGTATTTCCGGCTAGTATCATGGGGAGATTGAATGCTCTGGCAAGATTAAACATAGTACAGTGCGGCTCACTAAAAGTGGTATTTGAAGTCGGAGGAATTAATGTTGAAGAGACACGTGTCAACACATCATCCCTTTATGACCAGTTGAAACCTTTGTACTGTCAAAAACTTGAATTCTTGAGGATATGGAACGCTGGCAGTTTGAAAAATATATTCCCAGCCTCTTTGGCCAGAGGACTTCAGCAGCTACGATATCTGGACGTGATGTCTTGTCGTGATCTGCTGGAAATAATTGGTGAGGAAGAGGGAGGAGTAGAATTACCAACGCCTCCTCAGTTTGTGTTCCCAAAGGCAACATATGTGTCATTTTGGTCTCTGCCCCAACTGAAGAGTTTTTATCCGGGAAAGCATACTTCCACGTGGCCATTACTCAAACAATTGAGAGTAATATTACGAAGCGGAATTAATGAGGTAGAAATTTCAATCTTTCAGGATAAACATGAGTCGGGCCCAGTAAAGTCTATCTTTCCAATTGACAAGCTCTCCTATCTGCTATTTCCTGTTGACGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

1216

Amino Acids

138.67

Weight (kDa)

5.62

Isoelectric Point (pI)

37.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_16 PF13191 192 - 266 7e-07 AAA ATPase domain
ATPase_2 PF01637 192 - 302 3.1e-06 ATPase domain predominantly from Archaea
NB-ARC PF00931 195 - 355 3.8e-28 NB-ARC domain
AAA_22 PF13401 207 - 296 7.5e-06 AAA domain
LRR_14 PF23598 579 - 912 4e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 811 - 874 1e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 816 - 936 1.6e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 981 - 1048 2.3e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 984 - 1086 1.8e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1078 - 1118 2e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 76, 616, 1545
AccIII TCCGGA 1 cut(s) 1518
AciI CCGC 4 cut(s) 1080, 1972, 3141, 3537
AclWI GGATC 2 cut(s) 584, 2343
AcoI YGGCCR 3 cut(s) 1580, 3306, 3500
AcsI RAATTY 7 cut(s) 148, 275, 1728, 2756, 2964, 3251, 3555
AcuI CTGAAG 6 cut(s) 410, 570, 2153, 2388, 3302, 3485
AcvI CACGTG 2 cut(s) 3194, 3498
AcyI GRCGYC 4 cut(s) 73, 85, 613, 1542
AfaI GTAC 9 cut(s) 53, 732, 1315, 1421, 1551, 2746, 2839, 3135, 3236
AfiI CCNNNNNNNGG 2 cut(s) 529, 2726
AflII CTTAAG 2 cut(s) 1289, 1439
AflIII ACRYGT 4 cut(s) 1478, 2679, 3191, 3193
AhdI GACNNNNNGTC 1 cut(s) 3343
AhlI ACTAGT 2 cut(s) 670, 3043
AjiI CACGTC 1 cut(s) 3340
AjnI CCWGG 1 cut(s) 1619
AleI CACNNNNGTG 2 cut(s) 140, 671
Alw21I GWGCWC 1 cut(s) 2316
Alw26I GTCTC 7 cut(s) 401, 415, 604, 2088, 2456, 3182, 3456
AlwI GGATC 2 cut(s) 584, 2343
Ama87I CYCGRG 3 cut(s) 914, 1878, 2309
Aor13HI TCCGGA 1 cut(s) 1518
AoxI GGCC 8 cut(s) 334, 1021, 1580, 2376, 2882, 3306, 3500, 3586
ApaI GGGCCC 1 cut(s) 3590
ApeKI GCWGC 3 cut(s) 1285, 2916, 3322
ApoI RAATTY 7 cut(s) 148, 275, 1728, 2756, 2964, 3251, 3555
AseI ATTAAT 2 cut(s) 3177, 3543
Asp700I GAANNNNTTC 5 cut(s) 681, 762, 1683, 2715, 2807
AspS9I GGNCC 3 cut(s) 1623, 3586, 3587
AsuC2I CCSGG 1 cut(s) 3480
AsuHPI GGTGA 5 cut(s) 218, 1709, 1715, 2007, 3386
AsuII TTCGAA 1 cut(s) 1724
AvaI CYCGRG 3 cut(s) 914, 1878, 2309
AvaII GGWCC 1 cut(s) 1623
BaeGI GKGCMC 1 cut(s) 3590
BalI TGGCCA 3 cut(s) 1582, 3308, 3502
BanII GRGCYC 2 cut(s) 2316, 3590
BbrPI CACGTG 2 cut(s) 3194, 3498
BbsI GAAGAC 7 cut(s) 869, 1058, 1196, 1233, 1637, 1875, 2055
Bbv12I GWGCWC 1 cut(s) 2316
BbvCI CCTCAGC 1 cut(s) 2088
BbvI GCAGC 3 cut(s) 1297, 2903, 3334
BccI CCATC 5 cut(s) 191, 408, 1816, 1847, 2383
BceAI ACGGC 1 cut(s) 2248
BcgI CGANNNNNNTGC 2 cut(s) 2703, 2737
BciT130I CCWGG 1 cut(s) 1621
BclI TGATCA 3 cut(s) 1714, 2542, 3016
BcnI CCSGG 1 cut(s) 3480
BcoDI GTCTC 7 cut(s) 401, 415, 604, 2088, 2456, 3182, 3456
BcuI ACTAGT 2 cut(s) 670, 3043
BfrI CTTAAG 2 cut(s) 1289, 1439
BglI GCCNNNNNGGC 1 cut(s) 3305
BglII AGATCT 2 cut(s) 1614, 2363
BisI GCNGC 4 cut(s) 1286, 2917, 3142, 3323
BlsI GCNGC 4 cut(s) 1287, 2918, 3143, 3324
Bme1390I CCNGG 2 cut(s) 1621, 3480
Bme18I GGWCC 1 cut(s) 1623
BmeRI GACNNNNNGTC 1 cut(s) 3343
BmeT110I CYCGRG 3 cut(s) 914, 1878, 2309
BmgBI CACGTC 1 cut(s) 3340
BmgT120I GGNCC 3 cut(s) 1623, 3586, 3587
BmiI GGNNCC 1 cut(s) 3588
BmrFI CCNGG 2 cut(s) 1621, 3480
BmrI ACTGGG 3 cut(s) 612, 1635, 3584
BmsI GCATC 2 cut(s) 709, 1847
BmuI ACTGGG 3 cut(s) 612, 1635, 3584
BoxI GACNNNNGTC 1 cut(s) 3194
BpiI GAAGAC 7 cut(s) 869, 1058, 1196, 1233, 1637, 1875, 2055
BplI GAGNNNNNCTC 2 cut(s) 1255, 1287
BpmI CTGGAG 1 cut(s) 324
Bpu10I CCTNAGC 4 cut(s) 1093, 1671, 1998, 2088
Bpu14I TTCGAA 1 cut(s) 1724
BpuEI CTTGAG 7 cut(s) 123, 1086, 1748, 2074, 2545, 2624, 3277
BpuMI CCSGG 1 cut(s) 3480
BsaAI YACGTR 3 cut(s) 1479, 3194, 3498
BsaHI GRCGYC 4 cut(s) 73, 85, 613, 1542
BsaI GGTCTC 1 cut(s) 3456
BsaJI CCNNGG 5 cut(s) 1658, 1926, 2264, 2379, 2725
BsaWI WCCGGW 1 cut(s) 1518
BsaXI ACNNNNNCTCC 4 cut(s) 2189, 2219, 3605, 3635
Bsc4I CCNNNNNNNGG 2 cut(s) 529, 2726
Bse1I ACTGG 7 cut(s) 307, 618, 967, 1641, 2712, 3220, 3590
BseAI TCCGGA 1 cut(s) 1518
BseBI CCWGG 1 cut(s) 1621
BseDI CCNNGG 5 cut(s) 1658, 1926, 2264, 2379, 2725
BseGI GGATG 8 cut(s) 334, 478, 607, 886, 1069, 1862, 2389, 3206
BseLI CCNNNNNNNGG 2 cut(s) 529, 2726
BseMII CTCAG 6 cut(s) 266, 700, 1662, 1989, 2079, 3425
BseNI ACTGG 7 cut(s) 307, 618, 967, 1641, 2712, 3220, 3590
BseRI GAGGAG 3 cut(s) 2891, 3399, 3402
BseSI GKGCMC 1 cut(s) 3590
BseXI GCAGC 3 cut(s) 1297, 2903, 3334
BseYI CCCAGC 2 cut(s) 2321, 3295
BsgI GTGCAG 3 cut(s) 1627, 2423, 2722
BshFI GGCC 8 cut(s) 336, 1023, 1582, 2378, 2884, 3308, 3502, 3588
BsiHKAI GWGCWC 1 cut(s) 2316
BsiHKCI CYCGRG 3 cut(s) 914, 1878, 2309
BsiSI CCGG 3 cut(s) 1519, 3086, 3479
BslFI GGGAC 2 cut(s) 96, 600
BslI CCNNNNNNNGG 2 cut(s) 529, 2726
BsmAI GTCTC 7 cut(s) 401, 415, 604, 2088, 2456, 3182, 3456
BsmBI CGTCTC 3 cut(s) 401, 604, 2456
BsmFI GGGAC 2 cut(s) 96, 600
BsmI GAATGC 5 cut(s) 1494, 2119, 2529, 2807, 3115
BsnI GGCC 8 cut(s) 336, 1023, 1582, 2378, 2884, 3308, 3502, 3588
Bso31I GGTCTC 1 cut(s) 3456
BsoBI CYCGRG 3 cut(s) 914, 1878, 2309
Bsp119I TTCGAA 1 cut(s) 1724
Bsp120I GGGCCC 1 cut(s) 3586
Bsp1286I GDGCHC 2 cut(s) 2316, 3590
Bsp13I TCCGGA 1 cut(s) 1518
Bsp1407I TGTACA 1 cut(s) 1419
BspACI CCGC 4 cut(s) 1080, 1972, 3141, 3537
BspANI GGCC 8 cut(s) 336, 1023, 1582, 2378, 2884, 3308, 3502, 3588
BspCNI CTCAG 6 cut(s) 265, 699, 1663, 1990, 2080, 3424
BspEI TCCGGA 1 cut(s) 1518
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 3588
BspPI GGATC 2 cut(s) 584, 2343
BspQI GCTCTTC 1 cut(s) 342
BspT104I TTCGAA 1 cut(s) 1724
BspTI CTTAAG 2 cut(s) 1289, 1439
BspTNI GGTCTC 1 cut(s) 3456
BsrGI TGTACA 1 cut(s) 1419
BsrI ACTGG 7 cut(s) 307, 618, 967, 1641, 2712, 3220, 3590
BssECI CCNNGG 5 cut(s) 1658, 1926, 2264, 2379, 2725
BssNI GRCGYC 4 cut(s) 73, 85, 613, 1542
BssT1I CCWWGG 3 cut(s) 1658, 1926, 2379
Bst2UI CCWGG 1 cut(s) 1621
Bst4CI ACNGT 5 cut(s) 58, 515, 2069, 3138, 3239
Bst6I CTCTTC 7 cut(s) 196, 342, 2625, 2856, 3180, 3375, 3461
BstACI GRCGYC 4 cut(s) 73, 85, 613, 1542
BstAFI CTTAAG 2 cut(s) 1289, 1439
BstAUI TGTACA 1 cut(s) 1419
BstBAI YACGTR 3 cut(s) 1479, 3194, 3498
BstBI TTCGAA 1 cut(s) 1724
BstC8I GCNNGC 2 cut(s) 2690, 3274
BstDSI CCRYGG 1 cut(s) 2264
BstF5I GGATG 8 cut(s) 334, 478, 607, 886, 1069, 1862, 2389, 3206
BstMAI GTCTC 7 cut(s) 401, 415, 604, 2088, 2456, 3182, 3456
BstMWI GCNNNNNNNGC 5 cut(s) 1020, 1291, 1412, 2350, 3305
BstNI CCWGG 1 cut(s) 1621
BstNSI RCATGY 1 cut(s) 2683
BstPAI GACNNNNGTC 1 cut(s) 3194
BstSCI CCNGG 2 cut(s) 1619, 3478
BstSLI GKGCMC 1 cut(s) 3590
BstV1I GCAGC 3 cut(s) 1297, 2903, 3334
BstV2I GAAGAC 7 cut(s) 869, 1058, 1196, 1233, 1637, 1875, 2055
BstX2I RGATCY 3 cut(s) 576, 1614, 2363
BstXI CCANNNNNNTGG 3 cut(s) 302, 673, 1118
BstYI RGATCY 3 cut(s) 576, 1614, 2363
BsuRI GGCC 8 cut(s) 336, 1023, 1582, 2378, 2884, 3308, 3502, 3588
BtgI CCRYGG 1 cut(s) 2264
BtrI CACGTC 1 cut(s) 3340
BtsCI GGATG 8 cut(s) 334, 478, 607, 886, 1069, 1862, 2389, 3206
BtsIMutI CAGTG 3 cut(s) 520, 625, 3143
Cac8I GCNNGC 2 cut(s) 2690, 3274
CciI TCATGA 1 cut(s) 258
Cfr13I GGNCC 3 cut(s) 1623, 3586, 3587
CseI GACGC 3 cut(s) 93, 145, 383
Csp6I GTAC 9 cut(s) 52, 731, 1314, 1420, 1550, 2745, 2838, 3134, 3235
CspCI CAANNNNNGTGG 4 cut(s) 441, 476, 2181, 2216
CviQI GTAC 9 cut(s) 52, 731, 1314, 1420, 1550, 2745, 2838, 3134, 3235
DraI TTTAAA 2 cut(s) 1384, 1600
DriI GACNNNNNGTC 1 cut(s) 3343
EaeI YGGCCR 3 cut(s) 1580, 3306, 3500
Eam1104I CTCTTC 7 cut(s) 196, 342, 2625, 2856, 3180, 3375, 3461
Eam1105I GACNNNNNGTC 1 cut(s) 3343
EarI CTCTTC 7 cut(s) 196, 342, 2625, 2856, 3180, 3375, 3461
Ecl136II GAGCTC 1 cut(s) 2314
Eco130I CCWWGG 3 cut(s) 1658, 1926, 2379
Eco24I GRGCYC 2 cut(s) 2316, 3590
Eco31I GGTCTC 1 cut(s) 3456
Eco32I GATATC 3 cut(s) 2225, 2610, 3332
Eco47I GGWCC 1 cut(s) 1623
Eco53kI GAGCTC 1 cut(s) 2314
Eco57I CTGAAG 6 cut(s) 410, 570, 2153, 2388, 3302, 3485
Eco72I CACGTG 2 cut(s) 3194, 3498
Eco88I CYCGRG 3 cut(s) 914, 1878, 2309
EcoICRI GAGCTC 1 cut(s) 2314
EcoRI GAATTC 2 cut(s) 275, 3251
EcoRII CCWGG 1 cut(s) 1619
EcoRV GATATC 3 cut(s) 2225, 2610, 3332
EcoT14I CCWWGG 3 cut(s) 1658, 1926, 2379
EcoT22I ATGCAT 2 cut(s) 1417, 1494
EcoT38I GRGCYC 2 cut(s) 2316, 3590
ErhI CCWWGG 3 cut(s) 1658, 1926, 2379
Esp3I CGTCTC 3 cut(s) 401, 604, 2456
FalI AAGNNNNNCTT 2 cut(s) 3587, 3619
FaqI GGGAC 2 cut(s) 96, 600
FauNDI CATATG 1 cut(s) 3436
FbaI TGATCA 3 cut(s) 1714, 2542, 3016
Fnu4HI GCNGC 4 cut(s) 1286, 2917, 3142, 3323
FokI GGATG 8 cut(s) 341, 485, 614, 893, 1076, 1869, 2396, 3193
FriOI GRGCYC 2 cut(s) 2316, 3590
Fsp4HI GCNGC 4 cut(s) 1286, 2917, 3142, 3323
GluI GCNGC 4 cut(s) 1286, 2917, 3142, 3323
GsaI CCCAGC 2 cut(s) 2325, 3299
GsuI CTGGAG 1 cut(s) 324
HaeIII GGCC 8 cut(s) 336, 1023, 1582, 2378, 2884, 3308, 3502, 3588
HapII CCGG 3 cut(s) 1519, 3086, 3479
HgaI GACGC 3 cut(s) 93, 145, 383
Hin1I GRCGYC 4 cut(s) 73, 85, 613, 1542
HincII GTYRAC 4 cut(s) 450, 3049, 3199, 3643
HindII GTYRAC 4 cut(s) 450, 3049, 3199, 3643
HindIII AAGCTT 2 cut(s) 1242, 2690
HpaII CCGG 3 cut(s) 1519, 3086, 3479
HphI GGTGA 5 cut(s) 218, 1709, 1715, 2007, 3386
Hpy166II GTNNAC 7 cut(s) 54, 450, 1127, 1422, 3049, 3199, 3643
Hpy8I GTNNAC 7 cut(s) 54, 450, 1127, 1422, 3049, 3199, 3643
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 7 cut(s) 277, 319, 326, 2387, 2423, 2491, 2797
HpyCH4III ACNGT 5 cut(s) 58, 515, 2069, 3138, 3239
HpyF10VI GCNNNNNNNGC 5 cut(s) 1020, 1291, 1412, 2350, 3305
Hsp92I GRCGYC 4 cut(s) 73, 85, 613, 1542
Kpn2I TCCGGA 1 cut(s) 1518
Ksp22I TGATCA 3 cut(s) 1714, 2542, 3016
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 1 cut(s) 2128
Lsp1109I GCAGC 3 cut(s) 1297, 2903, 3334
LweI GCATC 2 cut(s) 709, 1847
MaeIII GTNAC 6 cut(s) 23, 694, 1066, 1265, 1797, 2816
MfeI CAATTG 3 cut(s) 18, 3515, 3609
MflI RGATCY 3 cut(s) 576, 1614, 2363
MhlI GDGCHC 2 cut(s) 2316, 3590
MlsI TGGCCA 3 cut(s) 1582, 3308, 3502
MluNI TGGCCA 3 cut(s) 1582, 3308, 3502
MlyI GAGTC 8 cut(s) 859, 1890, 1957, 2879, 3017, 3030, 3076, 3590
Mox20I TGGCCA 3 cut(s) 1582, 3308, 3502
Mph1103I ATGCAT 2 cut(s) 1417, 1494
MroI TCCGGA 1 cut(s) 1518
MroXI GAANNNNTTC 5 cut(s) 681, 762, 1683, 2715, 2807
MscI TGGCCA 3 cut(s) 1582, 3308, 3502
MslI CAYNNNNRTG 6 cut(s) 140, 222, 635, 671, 1698, 2901
Msp20I TGGCCA 3 cut(s) 1582, 3308, 3502
MspA1I CMGCKG 1 cut(s) 2353
MspCI CTTAAG 2 cut(s) 1289, 1439
MspI CCGG 3 cut(s) 1519, 3086, 3479
MspR9I CCNGG 2 cut(s) 1621, 3480
MunI CAATTG 3 cut(s) 18, 3515, 3609
Mva1269I GAATGC 5 cut(s) 1494, 2119, 2529, 2807, 3115
MvaI CCWGG 1 cut(s) 1621
MwoI GCNNNNNNNGC 5 cut(s) 1020, 1291, 1412, 2350, 3305
NciI CCSGG 1 cut(s) 3480
NdeI CATATG 1 cut(s) 3436
NlaIV GGNNCC 1 cut(s) 3588
NmeAIII GCCGAG 2 cut(s) 73, 990
NmuCI GTSAC 2 cut(s) 1797, 2816
NsiI ATGCAT 2 cut(s) 1417, 1494
NspI RCATGY 1 cut(s) 2683
NspV TTCGAA 1 cut(s) 1724
OliI CACNNNNGTG 2 cut(s) 140, 671
PaeR7I CTCGAG 2 cut(s) 1878, 2309
PagI TCATGA 1 cut(s) 258
PciI ACATGT 1 cut(s) 2679
PciSI GCTCTTC 1 cut(s) 342
PctI GAATGC 5 cut(s) 1494, 2119, 2529, 2807, 3115
PdmI GAANNNNTTC 5 cut(s) 681, 762, 1683, 2715, 2807
PfeI GAWTC 7 cut(s) 118, 569, 1076, 1592, 2553, 2733, 2844
PfoI TCCNGGA 1 cut(s) 3478
PkrI GCNGC 4 cut(s) 1287, 2918, 3143, 3324
PleI GAGTC 8 cut(s) 859, 1889, 1957, 2878, 3017, 3030, 3075, 3589
PmaCI CACGTG 2 cut(s) 3194, 3498
PmlI CACGTG 2 cut(s) 3194, 3498
PpsI GAGTC 8 cut(s) 859, 1889, 1957, 2878, 3017, 3030, 3075, 3589
Ppu21I YACGTR 3 cut(s) 1479, 3194, 3498
PscI ACATGT 1 cut(s) 2679
PshAI GACNNNNGTC 1 cut(s) 3194
PshBI ATTAAT 2 cut(s) 3177, 3543
Psp124BI GAGCTC 1 cut(s) 2316
Psp6I CCWGG 1 cut(s) 1619
PspCI CACGTG 2 cut(s) 3194, 3498
PspFI CCCAGC 2 cut(s) 2321, 3295
PspGI CCWGG 1 cut(s) 1619
PspN4I GGNNCC 1 cut(s) 3588
PspOMI GGGCCC 1 cut(s) 3586
PspPI GGNCC 3 cut(s) 1623, 3586, 3587
PspXI VCTCGAGB 2 cut(s) 1878, 2309
PsuI RGATCY 3 cut(s) 576, 1614, 2363
PvuII CAGCTG 1 cut(s) 2353
RsaI GTAC 9 cut(s) 53, 732, 1315, 1421, 1551, 2746, 2839, 3135, 3236
RsaNI GTAC 9 cut(s) 52, 731, 1314, 1420, 1550, 2745, 2838, 3134, 3235
RseI CAYNNNNRTG 6 cut(s) 140, 222, 635, 671, 1698, 2901
SacI GAGCTC 1 cut(s) 2316
SapI GCTCTTC 1 cut(s) 342
SatI GCNGC 4 cut(s) 1286, 2917, 3142, 3323
Sau96I GGNCC 3 cut(s) 1623, 3586, 3587
SchI GAGTC 8 cut(s) 859, 1890, 1957, 2879, 3017, 3030, 3076, 3590
ScrFI CCNGG 2 cut(s) 1621, 3480
SduI GDGCHC 2 cut(s) 2316, 3590
SfaNI GCATC 2 cut(s) 709, 1847
Sfr274I CTCGAG 2 cut(s) 1878, 2309
SfuI TTCGAA 1 cut(s) 1724
SinI GGWCC 1 cut(s) 1623
SlaI CTCGAG 2 cut(s) 1878, 2309
SmiMI CAYNNNNRTG 6 cut(s) 140, 222, 635, 671, 1698, 2901
SpeI ACTAGT 2 cut(s) 670, 3043
SsiI CCGC 4 cut(s) 1080, 1972, 3141, 3537
SspI AATATT 1 cut(s) 3528
SstI GAGCTC 1 cut(s) 2316
StyD4I CCNGG 2 cut(s) 1619, 3478
StyI CCWWGG 3 cut(s) 1658, 1926, 2379
TaaI ACNGT 5 cut(s) 58, 515, 2069, 3138, 3239
TaqI TCGA 7 cut(s) 240, 1273, 1408, 1724, 1879, 2046, 2310
TatI WGTACW 6 cut(s) 51, 1419, 1549, 2837, 3133, 3234
TauI GCSGC 1 cut(s) 3144
TfiI GAWTC 7 cut(s) 118, 569, 1076, 1592, 2553, 2733, 2844
TscAI CASTG 3 cut(s) 520, 625, 3143
TseFI GTSAC 2 cut(s) 1797, 2816
TseI GCWGC 3 cut(s) 1285, 2916, 3322
Tsp45I GTSAC 2 cut(s) 1797, 2816
TspGWI ACGGA 4 cut(s) 811, 1250, 1479, 2751
TspRI CASTG 3 cut(s) 520, 625, 3143
Vha464I CTTAAG 2 cut(s) 1289, 1439
VpaK11BI GGWCC 1 cut(s) 1623
VspI ATTAAT 2 cut(s) 3177, 3543
XapI RAATTY 7 cut(s) 148, 275, 1728, 2756, 2964, 3251, 3555
XbaI TCTAGA 1 cut(s) 956
XceI RCATGY 1 cut(s) 2683
XhoI CTCGAG 2 cut(s) 1878, 2309
XmnI GAANNNNTTC 5 cut(s) 681, 762, 1683, 2715, 2807
ZraI GACGTC 3 cut(s) 74, 614, 1543
Zsp2I ATGCAT 2 cut(s) 1417, 1494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.