MD01G1057500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001501
Physical Location & Seq
Reverse (-)
16216287 .. 16219099
2813 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1057500.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGAAGAACCAAAGCCAGCCGAGTCCACAGCCACCGAAATCCTCCCAACCCTTCCGAGCTCCGCCGAAGTCCTCCCTCAACAACCTCCGCCGTCTTCTCTTCCTTCCAAGACGAAGAAGAGACCTCTCGAAAATGATGCCCATTTCTCCAACTCATCTACCCTCTACAAGGTTCGCGCCGTCCTCAAAGAGGTCCGTCCTCATTTTCTCGAGGTTCTCCGAACCCCTGACTTTCGAACTTGCAAGTCATCCGAAGAAATTCAAGAATACGTTAAACTTCTGATGGAACTATGCAAACAGATGACGGCAGAAACAGTTTCGGCAGCAAGAAGTAAGAATGTGCCAGGTGAAAAGCCCCAAGATACAAAGGCTTTTGTAAAACCATCAGAAAATAAGTTTCCAACTGGTGTATCCAGCGAGAAGCAGCAGGCTGAAGATGGTCAGACTCAAGGGACATGTATTGTCGGTGGGTCTGCTTTTGGCTGGAATTTCATTACCTTTGTAGGCGAAGAACCAGTCTACTATGGAATAACAAAGGAGTCATTTCGAGCTAATGGCAGCAAAGAAACAGTTCAGACTGATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

21.57

Weight (kDa)

5.62

Isoelectric Point (pI)

54.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 519
AccII CGCG 1 cut(s) 177
AciI CCGC 2 cut(s) 63, 89
AcsI RAATTY 2 cut(s) 258, 487
AcuI CTGAAG 1 cut(s) 453
AfiI CCNNNNNNNGG 2 cut(s) 169, 190
AflIII ACRYGT 1 cut(s) 455
AgsI TTSAA 1 cut(s) 263
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 2 cut(s) 60, 551
AluI AGCT 2 cut(s) 60, 551
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 115
Ama87I CYCGRG 1 cut(s) 209
ApeKI GCWGC 3 cut(s) 323, 424, 558
ApoI RAATTY 2 cut(s) 258, 487
ArsI GACNNNNNNTTYG 2 cut(s) 501, 533
Asp700I GAANNNNTTC 2 cut(s) 258, 570
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 193
AsuHPI GGTGA 1 cut(s) 359
AsuII TTCGAA 1 cut(s) 235
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 1 cut(s) 193
BaeI ACNNNNGTAYC 2 cut(s) 393, 426
BanII GRGCYC 1 cut(s) 62
BbsI GAAGAC 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 62
BbvI GCAGC 3 cut(s) 335, 436, 570
BccI CCATC 3 cut(s) 277, 391, 431
BceAI ACGGC 3 cut(s) 76, 164, 321
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BciT130I CCWGG 1 cut(s) 345
BciVI GTATCC 1 cut(s) 421
BcoDI GTCTC 1 cut(s) 115
BfuI GTATCC 1 cut(s) 421
BisI GCNGC 3 cut(s) 324, 425, 559
BlsI GCNGC 3 cut(s) 325, 426, 560
Bme1390I CCNGG 1 cut(s) 345
Bme18I GGWCC 1 cut(s) 193
BmeT110I CYCGRG 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 193
BmrFI CCNGG 1 cut(s) 345
BmsI GCATC 1 cut(s) 127
BpiI GAAGAC 1 cut(s) 87
Bpu14I TTCGAA 1 cut(s) 235
BpuEI CTTGAG 1 cut(s) 432
BsaI GGTCTC 1 cut(s) 115
Bsc4I CCNNNNNNNGG 2 cut(s) 169, 190
Bse1I ACTGG 2 cut(s) 409, 515
BseBI CCWGG 1 cut(s) 345
BseGI GGATG 1 cut(s) 248
BseLI CCNNNNNNNGG 2 cut(s) 169, 190
BseNI ACTGG 2 cut(s) 409, 515
BseXI GCAGC 3 cut(s) 335, 436, 570
Bsh1236I CGCG 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 62
BsiHKCI CYCGRG 1 cut(s) 209
BslFI GGGAC 1 cut(s) 466
BslI CCNNNNNNNGG 2 cut(s) 169, 190
BsmAI GTCTC 1 cut(s) 115
BsmFI GGGAC 1 cut(s) 466
Bso31I GGTCTC 1 cut(s) 115
BsoBI CYCGRG 1 cut(s) 209
Bsp119I TTCGAA 1 cut(s) 235
Bsp1286I GDGCHC 1 cut(s) 62
BspACI CCGC 2 cut(s) 63, 89
BspFNI CGCG 1 cut(s) 177
BspT104I TTCGAA 1 cut(s) 235
BspTNI GGTCTC 1 cut(s) 115
BsrI ACTGG 2 cut(s) 409, 515
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 2 cut(s) 316, 571
Bst6I CTCTTC 2 cut(s) 105, 113
BstBI TTCGAA 1 cut(s) 235
BstC8I GCNNGC 2 cut(s) 18, 429
BstENI CCTNNNNNAGG 2 cut(s) 167, 188
BstF5I GGATG 1 cut(s) 248
BstFNI CGCG 1 cut(s) 177
BstHHI GCGC 1 cut(s) 179
BstMAI GTCTC 1 cut(s) 115
BstNI CCWGG 1 cut(s) 345
BstNSI RCATGY 1 cut(s) 459
BstSCI CCNGG 1 cut(s) 343
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 3 cut(s) 335, 436, 570
BstV2I GAAGAC 1 cut(s) 87
BsuI GTATCC 1 cut(s) 421
BtsCI GGATG 1 cut(s) 248
Cac8I GCNNGC 2 cut(s) 18, 429
CfoI GCGC 1 cut(s) 179
Cfr13I GGNCC 1 cut(s) 193
CviAII CATG 1 cut(s) 456
CviJI RGCY 9 cut(s) 16, 20, 32, 60, 355, 371, 431, 483, 551
CviKI_1 RGCY 9 cut(s) 16, 20, 32, 60, 355, 371, 431, 483, 551
Eam1104I CTCTTC 2 cut(s) 105, 113
EarI CTCTTC 2 cut(s) 105, 113
EciI GGCGGA 2 cut(s) 52, 78
Ecl136II GAGCTC 1 cut(s) 60
Eco24I GRGCYC 1 cut(s) 62
Eco31I GGTCTC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 193
Eco53kI GAGCTC 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 453
Eco88I CYCGRG 1 cut(s) 209
EcoICRI GAGCTC 1 cut(s) 60
EcoNI CCTNNNNNAGG 2 cut(s) 167, 188
EcoRII CCWGG 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 62
FaeI CATG 1 cut(s) 459
FaiI YATR 3 cut(s) 292, 457, 525
FaqI GGGAC 1 cut(s) 466
FatI CATG 1 cut(s) 455
FblI GTMKAC 1 cut(s) 519
Fnu4HI GCNGC 3 cut(s) 324, 425, 559
FokI GGATG 1 cut(s) 235
FriOI GRGCYC 1 cut(s) 62
Fsp4HI GCNGC 3 cut(s) 324, 425, 559
GlaI GCGC 1 cut(s) 178
GluI GCNGC 3 cut(s) 324, 425, 559
HhaI GCGC 1 cut(s) 179
Hin1II CATG 1 cut(s) 459
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HinfI GANTC 3 cut(s) 23, 445, 539
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 2 cut(s) 27, 520
Hpy188I TCNGA 7 cut(s) 57, 221, 253, 282, 388, 444, 576
Hpy188III TCNNGA 3 cut(s) 128, 209, 263
Hpy8I GTNNAC 2 cut(s) 27, 520
HpyAV CCTTC 2 cut(s) 62, 114
HpyCH4III ACNGT 2 cut(s) 316, 571
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 2 cut(s) 243, 294
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 1 cut(s) 459
HspAI GCGC 1 cut(s) 177
LmnI GCTCC 1 cut(s) 65
LpnPI CCDG 9 cut(s) 30, 240, 330, 357, 390, 413, 427, 469, 528
Lsp1109I GCAGC 3 cut(s) 335, 436, 570
LweI GCATC 1 cut(s) 127
MaeII ACGT 1 cut(s) 270
MboII GAAGA 8 cut(s) 17, 87, 92, 127, 130, 266, 446, 521
MhlI GDGCHC 1 cut(s) 62
MluCI AATT 2 cut(s) 258, 487
MlyI GAGTC 3 cut(s) 32, 439, 548
MmeI TCCRAC 2 cut(s) 174, 425
MroXI GAANNNNTTC 2 cut(s) 258, 570
MseI TTAA 1 cut(s) 273
MspR9I CCNGG 1 cut(s) 345
MvaI CCWGG 1 cut(s) 345
MvnI CGCG 1 cut(s) 177
NlaIII CATG 1 cut(s) 459
NmeAIII GCCGAG 1 cut(s) 46
NspI RCATGY 1 cut(s) 459
NspV TTCGAA 1 cut(s) 235
PaeR7I CTCGAG 1 cut(s) 209
PciI ACATGT 1 cut(s) 455
PdmI GAANNNNTTC 2 cut(s) 258, 570
PkrI GCNGC 3 cut(s) 325, 426, 560
PleI GAGTC 3 cut(s) 31, 439, 547
PpsI GAGTC 3 cut(s) 31, 439, 547
PscI ACATGT 1 cut(s) 455
Psp124BI GAGCTC 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspPI GGNCC 1 cut(s) 193
SacI GAGCTC 1 cut(s) 62
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 3 cut(s) 324, 425, 559
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 3 cut(s) 32, 439, 548
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 1 cut(s) 62
SfaNI GCATC 1 cut(s) 127
Sfr274I CTCGAG 1 cut(s) 209
SfuI TTCGAA 1 cut(s) 235
SinI GGWCC 1 cut(s) 193
SlaI CTCGAG 1 cut(s) 209
SmlI CTYRAG 2 cut(s) 209, 447
SmoI CTYRAG 2 cut(s) 209, 447
Sse9I AATT 2 cut(s) 258, 487
SsiI CCGC 2 cut(s) 63, 89
SstI GAGCTC 1 cut(s) 62
StyD4I CCNGG 1 cut(s) 343
TaaI ACNGT 2 cut(s) 316, 571
TaiI ACGT 1 cut(s) 273
TaqI TCGA 4 cut(s) 129, 210, 235, 547
TasI AATT 2 cut(s) 258, 487
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TseI GCWGC 3 cut(s) 323, 424, 558
TspDTI ATGAA 1 cut(s) 481
TspGWI ACGGA 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 193
XagI CCTNNNNNAGG 2 cut(s) 167, 188
XapI RAATTY 2 cut(s) 258, 487
XceI RCATGY 1 cut(s) 459
XhoI CTCGAG 1 cut(s) 209
XmiI GTMKAC 1 cut(s) 519
XmnI GAANNNNTTC 2 cut(s) 258, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.