pycom01g21180

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001501
Physical Location & Seq
Reverse (-)
19339702 .. 19340898
1197 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21180.2

Sequence Viewer

Length: 738 bp
ATGGAAGTTGCAAAACCAAAAGGTGTTGAGATCGCAAAGGGACACGTAACAGCAAGGCCACATGGCGCCCCACGACCACCGAGCTCCGCCCAACAAAGACCCAAACCCAAATCCACCACCACTTCCTCTCTTCCTCCTTGCCGCAAGAAACGAAAGCCCACCACCAATTTCAACATGGACCTCCACCACCACCACCACTCCCCACATTTCAAAATTCGCGCTCTCGTTCATCGGCTTCGTCCCCGATTTATTGAGGTTCTTCGAACACCGGATTTTCGGAATTGTAAAGCGGCTGATGAAATTCGAAAGGAACTGAATCTTTTGATGGAGCTACACAAACAGATGGCCAAGGATACCGGCACACAGGGGAAGTTTGTGCCAGAACATCAGCATCCGGCACGTGACAGCGAGCCTGCGAAAGAAAACCAAGATGGGAAAGGGCCGCAGCAGCTTCAACCAAGGTGCTCAGTTCAAAATTCTGTACAAGGTAACAGTACTCGACCGGCTCCTCTCAGCGCAAATAAGAAACCAGTTGATTGCAAACTACAGGGAAGCTACATTGTGGGCGGTTCAGCATTTGGTTGGAACTTCATCACATTTTCCGCAGACGGCACTAAACCAGTCTACTGTGGGGTGACGAAGGAGGCACATCGAAGCAGCAACGATCGATTGGGAGACGCTAATCTGGCCATAACGACCGGGTTTTCTGATAATTGTACACCGTCTATAGCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

246

Amino Acids

27.08

Weight (kDa)

9.86

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 65
AccI GTMKAC 1 cut(s) 624
AccII CGCG 1 cut(s) 219
AciI CCGC 6 cut(s) 87, 142, 290, 443, 567, 603
AcoI YGGCCR 2 cut(s) 345, 687
AcsI RAATTY 3 cut(s) 213, 300, 475
AcvI CACGTG 1 cut(s) 401
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 3 cut(s) 483, 496, 718
AflIII ACRYGT 1 cut(s) 43
AgsI TTSAA 4 cut(s) 172, 211, 455, 473
AluBI AGCT 4 cut(s) 84, 331, 451, 555
AluI AGCT 4 cut(s) 84, 331, 451, 555
Alw21I GWGCWC 2 cut(s) 86, 467
Alw26I GTCTC 1 cut(s) 669
AoxI GGCC 4 cut(s) 56, 345, 440, 687
ApeKI GCWGC 3 cut(s) 445, 448, 657
ApoI RAATTY 3 cut(s) 213, 300, 475
AspLEI GCGC 3 cut(s) 68, 221, 518
AspS9I GGNCC 2 cut(s) 178, 440
AsuC2I CCSGG 1 cut(s) 700
AsuHPI GGTGA 1 cut(s) 646
AsuII TTCGAA 2 cut(s) 262, 304
AvaII GGWCC 1 cut(s) 178
BalI TGGCCA 2 cut(s) 347, 689
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 86
BbrPI CACGTG 1 cut(s) 401
Bbv12I GWGCWC 2 cut(s) 86, 467
BbvI GCAGC 3 cut(s) 457, 460, 669
BccI CCATC 3 cut(s) 319, 337, 425
BceAI ACGGC 1 cut(s) 625
BciVI GTATCC 1 cut(s) 346
BcnI CCSGG 1 cut(s) 700
BcoDI GTCTC 1 cut(s) 669
BfmI CTRYAG 2 cut(s) 545, 726
BfoI RGCGCY 1 cut(s) 69
BfuI GTATCC 1 cut(s) 346
BisI GCNGC 6 cut(s) 142, 291, 443, 446, 449, 658
BlsI GCNGC 6 cut(s) 143, 292, 444, 447, 450, 659
BmcAI AGTACT 1 cut(s) 496
Bme1390I CCNGG 1 cut(s) 700
Bme18I GGWCC 1 cut(s) 178
BmgT120I GGNCC 2 cut(s) 178, 440
BmiI GGNNCC 2 cut(s) 67, 507
BmrFI CCNGG 1 cut(s) 700
BmsI GCATC 1 cut(s) 400
Bpu14I TTCGAA 2 cut(s) 262, 304
BpuMI CCSGG 1 cut(s) 700
Bsa29I ATCGAT 1 cut(s) 667
BsaAI YACGTR 2 cut(s) 46, 401
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 2 cut(s) 348, 458
BsaWI WCCGGW 1 cut(s) 268
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bse118I RCCGGY 2 cut(s) 356, 502
Bse1I ACTGG 2 cut(s) 530, 620
BseCI ATCGAT 1 cut(s) 667
BseDI CCNNGG 2 cut(s) 348, 458
BseGI GGATG 1 cut(s) 391
BseMII CTCAG 2 cut(s) 480, 526
BseNI ACTGG 2 cut(s) 530, 620
BseRI GAGGAG 1 cut(s) 498
BseXI GCAGC 3 cut(s) 457, 460, 669
Bsh1236I CGCG 1 cut(s) 219
Bsh1285I CGRYCG 3 cut(s) 503, 667, 699
BshFI GGCC 4 cut(s) 58, 347, 442, 689
BshNI GGYRCC 1 cut(s) 65
BshVI ATCGAT 1 cut(s) 667
BsiEI CGRYCG 3 cut(s) 503, 667, 699
BsiHKAI GWGCWC 2 cut(s) 86, 467
BsiSI CCGG 5 cut(s) 269, 357, 395, 503, 699
BslFI GGGAC 2 cut(s) 54, 225
BsmAI GTCTC 1 cut(s) 669
BsmBI CGTCTC 1 cut(s) 669
BsmFI GGGAC 2 cut(s) 54, 225
BsnI GGCC 4 cut(s) 58, 347, 442, 689
Bsp119I TTCGAA 2 cut(s) 262, 304
Bsp1286I GDGCHC 2 cut(s) 86, 467
Bsp1407I TGTACA 2 cut(s) 481, 716
Bsp143I GATC 2 cut(s) 30, 664
BspACI CCGC 6 cut(s) 87, 142, 290, 443, 567, 603
BspANI GGCC 4 cut(s) 58, 347, 442, 689
BspCNI CTCAG 2 cut(s) 479, 525
BspDI ATCGAT 1 cut(s) 667
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 2 cut(s) 67, 507
BspT104I TTCGAA 2 cut(s) 262, 304
BspT107I GGYRCC 1 cut(s) 65
BsrFI RCCGGY 2 cut(s) 356, 502
BsrGI TGTACA 2 cut(s) 481, 716
BsrI ACTGG 2 cut(s) 530, 620
BssAI RCCGGY 2 cut(s) 356, 502
BssECI CCNNGG 2 cut(s) 348, 458
BssMI GATC 2 cut(s) 30, 664
BssNI GRCGYC 1 cut(s) 66
BssT1I CCWWGG 2 cut(s) 348, 458
Bst4CI ACNGT 3 cut(s) 494, 629, 723
Bst6I CTCTTC 1 cut(s) 135
BstACI GRCGYC 1 cut(s) 66
BstAUI TGTACA 2 cut(s) 481, 716
BstBAI YACGTR 2 cut(s) 46, 401
BstBI TTCGAA 2 cut(s) 262, 304
BstC8I GCNNGC 2 cut(s) 410, 414
BstDEI CTNAG 2 cut(s) 466, 512
BstF5I GGATG 1 cut(s) 391
BstFNI CGCG 1 cut(s) 219
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 3 cut(s) 68, 221, 518
BstKTI GATC 2 cut(s) 33, 667
BstMAI GTCTC 1 cut(s) 669
BstMBI GATC 2 cut(s) 30, 664
BstMCI CGRYCG 3 cut(s) 503, 667, 699
BstMWI GCNNNNNNNGC 2 cut(s) 448, 686
BstSCI CCNGG 1 cut(s) 698
BstSFI CTRYAG 2 cut(s) 545, 726
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 3 cut(s) 457, 460, 669
Bsu15I ATCGAT 1 cut(s) 667
BsuI GTATCC 1 cut(s) 346
BsuRI GGCC 4 cut(s) 58, 347, 442, 689
BsuTUI ATCGAT 1 cut(s) 667
BtsCI GGATG 1 cut(s) 391
Cac8I GCNNGC 2 cut(s) 410, 414
CfoI GCGC 3 cut(s) 68, 221, 518
Cfr10I RCCGGY 2 cut(s) 356, 502
Cfr13I GGNCC 2 cut(s) 178, 440
ClaI ATCGAT 1 cut(s) 667
CseI GACGC 1 cut(s) 686
Csp6I GTAC 3 cut(s) 482, 495, 717
CviAII CATG 2 cut(s) 62, 175
CviQI GTAC 3 cut(s) 482, 495, 717
DdeI CTNAG 2 cut(s) 466, 512
DinI GGCGCC 1 cut(s) 67
DpnI GATC 2 cut(s) 32, 666
DpnII GATC 2 cut(s) 30, 664
EaeI YGGCCR 2 cut(s) 345, 687
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
EciI GGCGGA 1 cut(s) 76
Ecl136II GAGCTC 1 cut(s) 84
Eco130I CCWWGG 2 cut(s) 348, 458
Eco24I GRGCYC 1 cut(s) 86
Eco47I GGWCC 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 84
Eco72I CACGTG 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 84
EcoT14I CCWWGG 2 cut(s) 348, 458
EcoT38I GRGCYC 1 cut(s) 86
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
ErhI CCWWGG 2 cut(s) 348, 458
Esp3I CGTCTC 1 cut(s) 669
FaeI CATG 2 cut(s) 65, 178
FaiI YATR 4 cut(s) 63, 176, 692, 728
FaqI GGGAC 2 cut(s) 54, 225
FatI CATG 2 cut(s) 61, 174
FblI GTMKAC 1 cut(s) 624
Fnu4HI GCNGC 6 cut(s) 142, 291, 443, 446, 449, 658
FokI GGATG 1 cut(s) 378
FriOI GRGCYC 1 cut(s) 86
Fsp4HI GCNGC 6 cut(s) 142, 291, 443, 446, 449, 658
GlaI GCGC 3 cut(s) 67, 220, 517
GluI GCNGC 6 cut(s) 142, 291, 443, 446, 449, 658
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 4 cut(s) 58, 347, 442, 689
HapII CCGG 5 cut(s) 269, 357, 395, 503, 699
HgaI GACGC 1 cut(s) 686
HhaI GCGC 3 cut(s) 68, 221, 518
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 2 cut(s) 65, 178
Hin6I GCGC 3 cut(s) 66, 219, 516
HinP1I GCGC 3 cut(s) 66, 219, 516
HinfI GANTC 1 cut(s) 316
HpaII CCGG 5 cut(s) 269, 357, 395, 503, 699
HphI GGTGA 1 cut(s) 646
Hpy166II GTNNAC 2 cut(s) 625, 719
Hpy188I TCNGA 2 cut(s) 279, 709
Hpy8I GTNNAC 2 cut(s) 625, 719
HpyAV CCTTC 1 cut(s) 634
HpyCH4III ACNGT 3 cut(s) 494, 629, 723
HpyCH4IV ACGT 2 cut(s) 45, 400
HpyCH4V TGCA 2 cut(s) 11, 540
HpyF10VI GCNNNNNNNGC 2 cut(s) 448, 686
HpyF3I CTNAG 2 cut(s) 466, 512
HpySE526I ACGT 2 cut(s) 45, 400
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 2 cut(s) 65, 178
HspAI GCGC 3 cut(s) 66, 219, 516
KasI GGCGCC 1 cut(s) 65
Kzo9I GATC 2 cut(s) 30, 664
LmnI GCTCC 3 cut(s) 89, 328, 511
Lsp1109I GCAGC 3 cut(s) 457, 460, 669
LweI GCATC 1 cut(s) 400
MaeII ACGT 2 cut(s) 45, 400
MaeIII GTNAC 4 cut(s) 46, 401, 488, 634
MalI GATC 2 cut(s) 32, 666
MboI GATC 2 cut(s) 30, 664
MboII GAAGA 2 cut(s) 122, 251
MhlI GDGCHC 2 cut(s) 86, 467
MlsI TGGCCA 2 cut(s) 347, 689
MluCI AATT 6 cut(s) 166, 213, 280, 300, 475, 712
MluNI TGGCCA 2 cut(s) 347, 689
Mly113I GGCGCC 1 cut(s) 66
MmeI TCCRAC 1 cut(s) 563
MnlI CCTC 6 cut(s) 136, 144, 191, 247, 519, 637
Mox20I TGGCCA 2 cut(s) 347, 689
MscI TGGCCA 2 cut(s) 347, 689
Msp20I TGGCCA 2 cut(s) 347, 689
MspI CCGG 5 cut(s) 269, 357, 395, 503, 699
MspR9I CCNGG 1 cut(s) 700
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 2 cut(s) 448, 686
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 1 cut(s) 700
NdeII GATC 2 cut(s) 30, 664
NlaIII CATG 2 cut(s) 65, 178
NlaIV GGNNCC 2 cut(s) 67, 507
NmuCI GTSAC 2 cut(s) 401, 634
NspV TTCGAA 2 cut(s) 262, 304
PfeI GAWTC 1 cut(s) 316
PkrI GCNGC 6 cut(s) 143, 292, 444, 447, 450, 659
Ple19I CGATCG 1 cut(s) 667
PluTI GGCGCC 1 cut(s) 69
PmaCI CACGTG 1 cut(s) 401
PmlI CACGTG 1 cut(s) 401
Ppu21I YACGTR 2 cut(s) 46, 401
Psp124BI GAGCTC 1 cut(s) 86
PspCI CACGTG 1 cut(s) 401
PspN4I GGNNCC 2 cut(s) 67, 507
PspPI GGNCC 2 cut(s) 178, 440
PvuI CGATCG 1 cut(s) 667
RsaI GTAC 3 cut(s) 483, 496, 718
RsaNI GTAC 3 cut(s) 482, 495, 717
SacI GAGCTC 1 cut(s) 86
SatI GCNGC 6 cut(s) 142, 291, 443, 446, 449, 658
Sau3AI GATC 2 cut(s) 30, 664
Sau96I GGNCC 2 cut(s) 178, 440
ScaI AGTACT 1 cut(s) 496
ScrFI CCNGG 1 cut(s) 700
SduI GDGCHC 2 cut(s) 86, 467
SfaNI GCATC 1 cut(s) 400
SfcI CTRYAG 2 cut(s) 545, 726
SfoI GGCGCC 1 cut(s) 67
SfuI TTCGAA 2 cut(s) 262, 304
SinI GGWCC 1 cut(s) 178
Sse9I AATT 6 cut(s) 166, 213, 280, 300, 475, 712
SsiI CCGC 6 cut(s) 87, 142, 290, 443, 567, 603
SspDI GGCGCC 1 cut(s) 65
SstI GAGCTC 1 cut(s) 86
StyD4I CCNGG 1 cut(s) 698
StyI CCWWGG 2 cut(s) 348, 458
TaaI ACNGT 3 cut(s) 494, 629, 723
TaiI ACGT 2 cut(s) 48, 403
TaqI TCGA 5 cut(s) 262, 304, 499, 652, 667
TasI AATT 6 cut(s) 166, 213, 280, 300, 475, 712
TatI WGTACW 3 cut(s) 481, 494, 716
TauI GCSGC 3 cut(s) 144, 293, 445
TfiI GAWTC 1 cut(s) 316
TseFI GTSAC 2 cut(s) 401, 634
TseI GCWGC 3 cut(s) 445, 448, 657
Tsp45I GTSAC 2 cut(s) 401, 634
TspDTI ATGAA 3 cut(s) 218, 312, 580
VpaK11BI GGWCC 1 cut(s) 178
XapI RAATTY 3 cut(s) 213, 300, 475
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XmiI GTMKAC 1 cut(s) 624
ZrmI AGTACT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.