MD07G1139200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001501
Physical Location & Seq
Forward (+)
20185414 .. 20189045
3632 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1139200.v1.1.491

Sequence Viewer

Length: 591 bp
ATGGAGGAAGAACCAAAGCCAGCCGAGTCCACAGCCACCGAAATCCTCCCAATCCCTCCGAGCTCCGCCGAAGTCCTCCCTCCACAGGCTCCTCCGTCTTCTCTTCCTCCCAAGTCCAAGAAGAGACCTCTTGAAAACGATGCTCATTTCTCCAACTCATCCACCCTCTACAAGATTCGCGCCGTCCTCAAAGAGGTCCGCCCTCATTTTCTCGAGGTCCTCGGGACCCCTGACTTTCGAACGTGCAAGTCATCTGAAGAAATTCAAGAATATGTTAAACTTCTGATGGAACTATGCAAACAGATGACGGCAGAAACAGTTTCGGCAACAAGAAGTAAGAATGTGCCAGGTGAAAAGCCCCAAGATACAAGGGCTTTTGCAAAGCCATCAGAAAATAAGTTTCCAACAGGTGTATCCACTGAAAAGCAGCGGGCTGAAGATGGTCAGACTCAAGGGACATATATTGTTGGTGGGTCTGCGTTCGGCTGGAATTTCATTACCTTTGTAGGCGAAGAACCAGTCTACTACGGAATAACAAAGGAGTCATTTCGAGCTAATGGCAGCAAAGAAACAGTTCAGACTATGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

21.7

Weight (kDa)

5.63

Isoelectric Point (pI)

51.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 522
AccII CGCG 1 cut(s) 180
AciI CCGC 3 cut(s) 66, 199, 430
AcsI RAATTY 2 cut(s) 261, 490
AcuI CTGAAG 2 cut(s) 276, 456
AfiI CCNNNNNNNGG 2 cut(s) 85, 193
AgsI TTSAA 2 cut(s) 134, 266
AjnI CCWGG 1 cut(s) 346
AluBI AGCT 2 cut(s) 63, 554
AluI AGCT 2 cut(s) 63, 554
Alw21I GWGCWC 1 cut(s) 65
Alw26I GTCTC 1 cut(s) 118
Ama87I CYCGRG 2 cut(s) 212, 221
ApeKI GCWGC 2 cut(s) 427, 561
ApoI RAATTY 2 cut(s) 261, 490
ArsI GACNNNNNNTTYG 2 cut(s) 504, 536
Asp700I GAANNNNTTC 2 cut(s) 261, 573
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 3 cut(s) 196, 217, 225
AsuHPI GGTGA 1 cut(s) 362
AsuII TTCGAA 1 cut(s) 238
AvaI CYCGRG 2 cut(s) 212, 221
AvaII GGWCC 3 cut(s) 196, 217, 225
BaeI ACNNNNGTAYC 2 cut(s) 396, 429
BanII GRGCYC 1 cut(s) 65
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 65
BbvI GCAGC 2 cut(s) 439, 573
BccI CCATC 3 cut(s) 280, 394, 434
BceAI ACGGC 2 cut(s) 167, 324
BciT130I CCWGG 1 cut(s) 348
BciVI GTATCC 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 118
BfuI GTATCC 1 cut(s) 424
BisI GCNGC 2 cut(s) 428, 562
BlsI GCNGC 2 cut(s) 429, 563
Bme1390I CCNGG 1 cut(s) 348
Bme18I GGWCC 3 cut(s) 196, 217, 225
BmeT110I CYCGRG 2 cut(s) 212, 221
BmgT120I GGNCC 3 cut(s) 196, 217, 225
BmiI GGNNCC 3 cut(s) 90, 226, 227
BmrFI CCNGG 1 cut(s) 348
BmsI GCATC 1 cut(s) 130
BpiI GAAGAC 1 cut(s) 90
Bpu14I TTCGAA 1 cut(s) 238
BpuEI CTTGAG 1 cut(s) 435
BsaI GGTCTC 1 cut(s) 118
BsaJI CCNNGG 1 cut(s) 220
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 193
Bse1I ACTGG 1 cut(s) 518
BseBI CCWGG 1 cut(s) 348
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 1 cut(s) 158
BseLI CCNNNNNNNGG 2 cut(s) 85, 193
BseNI ACTGG 1 cut(s) 518
BseRI GAGGAG 1 cut(s) 81
BseXI GCAGC 2 cut(s) 439, 573
Bsh1236I CGCG 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 65
BsiHKCI CYCGRG 2 cut(s) 212, 221
BslFI GGGAC 2 cut(s) 238, 469
BslI CCNNNNNNNGG 2 cut(s) 85, 193
BsmAI GTCTC 1 cut(s) 118
BsmFI GGGAC 2 cut(s) 238, 469
Bso31I GGTCTC 1 cut(s) 118
BsoBI CYCGRG 2 cut(s) 212, 221
Bsp119I TTCGAA 1 cut(s) 238
Bsp1286I GDGCHC 1 cut(s) 65
BspACI CCGC 3 cut(s) 66, 199, 430
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 3 cut(s) 90, 226, 227
BspT104I TTCGAA 1 cut(s) 238
BspTNI GGTCTC 1 cut(s) 118
BsrI ACTGG 1 cut(s) 518
BssECI CCNNGG 1 cut(s) 220
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 2 cut(s) 319, 574
Bst6I CTCTTC 2 cut(s) 108, 116
BstBI TTCGAA 1 cut(s) 238
BstC8I GCNNGC 2 cut(s) 21, 432
BstENI CCTNNNNNAGG 1 cut(s) 191
BstF5I GGATG 1 cut(s) 158
BstFNI CGCG 1 cut(s) 180
BstHHI GCGC 1 cut(s) 182
BstMAI GTCTC 1 cut(s) 118
BstNI CCWGG 1 cut(s) 348
BstSCI CCNGG 1 cut(s) 346
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 2 cut(s) 439, 573
BstV2I GAAGAC 1 cut(s) 90
BsuI GTATCC 1 cut(s) 424
BtsCI GGATG 1 cut(s) 158
BtsIMutI CAGTG 1 cut(s) 417
Cac8I GCNNGC 2 cut(s) 21, 432
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 3 cut(s) 196, 217, 225
Eam1104I CTCTTC 2 cut(s) 108, 116
EarI CTCTTC 2 cut(s) 108, 116
EciI GGCGGA 2 cut(s) 55, 188
Ecl136II GAGCTC 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 65
Eco31I GGTCTC 1 cut(s) 118
Eco47I GGWCC 3 cut(s) 196, 217, 225
Eco53kI GAGCTC 1 cut(s) 63
Eco57I CTGAAG 2 cut(s) 276, 456
Eco88I CYCGRG 2 cut(s) 212, 221
EcoICRI GAGCTC 1 cut(s) 63
EcoNI CCTNNNNNAGG 1 cut(s) 191
EcoO109I RGGNCCY 2 cut(s) 217, 225
EcoRII CCWGG 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 65
FaiI YATR 5 cut(s) 273, 295, 460, 462, 584
FaqI GGGAC 2 cut(s) 238, 469
FauI CCCGC 1 cut(s) 423
FblI GTMKAC 1 cut(s) 522
Fnu4HI GCNGC 2 cut(s) 428, 562
FokI GGATG 1 cut(s) 145
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 2 cut(s) 428, 562
GlaI GCGC 1 cut(s) 181
GluI GCNGC 2 cut(s) 428, 562
HhaI GCGC 1 cut(s) 182
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HinfI GANTC 4 cut(s) 26, 175, 448, 542
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 2 cut(s) 30, 523
Hpy188I TCNGA 6 cut(s) 60, 256, 285, 391, 447, 579
Hpy188III TCNNGA 4 cut(s) 131, 212, 223, 266
Hpy8I GTNNAC 2 cut(s) 30, 523
HpyCH4III ACNGT 2 cut(s) 319, 574
HpyCH4IV ACGT 1 cut(s) 242
HpyCH4V TGCA 3 cut(s) 246, 297, 380
HpySE526I ACGT 1 cut(s) 242
HspAI GCGC 1 cut(s) 180
KflI GGGWCCC 1 cut(s) 225
LmnI GCTCC 2 cut(s) 68, 94
LpnPI CCDG 8 cut(s) 33, 71, 243, 333, 360, 393, 472, 531
Lsp1109I GCAGC 2 cut(s) 439, 573
LweI GCATC 1 cut(s) 130
MaeII ACGT 1 cut(s) 242
MboII GAAGA 7 cut(s) 20, 90, 95, 133, 269, 449, 524
MhlI GDGCHC 1 cut(s) 65
MluCI AATT 2 cut(s) 261, 490
MlyI GAGTC 3 cut(s) 35, 442, 551
MmeI TCCRAC 2 cut(s) 177, 428
MroXI GAANNNNTTC 2 cut(s) 261, 573
MseI TTAA 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 430
MspR9I CCNGG 1 cut(s) 348
MvaI CCWGG 1 cut(s) 348
MvnI CGCG 1 cut(s) 180
NlaIV GGNNCC 3 cut(s) 90, 226, 227
NmeAIII GCCGAG 1 cut(s) 49
NspV TTCGAA 1 cut(s) 238
PaeR7I CTCGAG 1 cut(s) 212
PdmI GAANNNNTTC 2 cut(s) 261, 573
PfeI GAWTC 1 cut(s) 175
PkrI GCNGC 2 cut(s) 429, 563
PleI GAGTC 3 cut(s) 34, 442, 550
PpsI GAGTC 3 cut(s) 34, 442, 550
PpuMI RGGWCCY 2 cut(s) 217, 225
Psp124BI GAGCTC 1 cut(s) 65
Psp5II RGGWCCY 2 cut(s) 217, 225
Psp6I CCWGG 1 cut(s) 346
PspGI CCWGG 1 cut(s) 346
PspN4I GGNNCC 3 cut(s) 90, 226, 227
PspPI GGNCC 3 cut(s) 196, 217, 225
PspPPI RGGWCCY 2 cut(s) 217, 225
SacI GAGCTC 1 cut(s) 65
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 2 cut(s) 428, 562
Sau96I GGNCC 3 cut(s) 196, 217, 225
SchI GAGTC 3 cut(s) 35, 442, 551
ScrFI CCNGG 1 cut(s) 348
SduI GDGCHC 1 cut(s) 65
SetI ASST 9 cut(s) 65, 130, 198, 219, 245, 352, 412, 503, 556
SfaNI GCATC 1 cut(s) 130
Sfr274I CTCGAG 1 cut(s) 212
SfuI TTCGAA 1 cut(s) 238
SinI GGWCC 3 cut(s) 196, 217, 225
SlaI CTCGAG 1 cut(s) 212
SmlI CTYRAG 2 cut(s) 212, 450
SmoI CTYRAG 2 cut(s) 212, 450
Sse9I AATT 2 cut(s) 261, 490
SsiI CCGC 3 cut(s) 66, 199, 430
SstI GAGCTC 1 cut(s) 65
StyD4I CCNGG 1 cut(s) 346
TaaI ACNGT 2 cut(s) 319, 574
TaiI ACGT 1 cut(s) 245
TaqI TCGA 3 cut(s) 213, 238, 550
TasI AATT 2 cut(s) 261, 490
TfiI GAWTC 1 cut(s) 175
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 1 cut(s) 424
TseI GCWGC 2 cut(s) 427, 561
TspDTI ATGAA 1 cut(s) 484
TspGWI ACGGA 2 cut(s) 84, 543
TspRI CASTG 1 cut(s) 424
VpaK11BI GGWCC 3 cut(s) 196, 217, 225
XagI CCTNNNNNAGG 1 cut(s) 191
XapI RAATTY 2 cut(s) 261, 490
XhoI CTCGAG 1 cut(s) 212
XmiI GTMKAC 1 cut(s) 522
XmnI GAANNNNTTC 2 cut(s) 261, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.