Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001122

Physical Location & Seq

Genomic Coordinates Reverse (-)

5612264 .. 5614672

Gene Structure 2409 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1069300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1002 bp

ATGACTATCCTCATTGAGCAGCCTGAGCTAGGGTTACGAGTGGAGGAAAAGAAGATTATTTCTTCAGAGAACAATGGAGAAATGAATGATTTAGTATTAGATGGTGGGTTTGTGGTGCCCAAAGAGATCTCAAAAAATGATGGAACTGTGGCACAAGAAATTACAGGATCTAATGAGGCCTTCATTGCACTTGAAATCAATTCATTTGGCCAGTCGTTCAGGGATTATAATGCAGAAAGCGAAAGGCAGAAATCCGTGGAGGAATTCTACCGGTTGATCCACATTAATCAAACGCATGATTTCGTGAAGAGGATGAGGGAGGAGTACAGCAAGTTGAATCGAGTGGAAATGAGCATATGGGAATGTTGTGAGCTGCTCAATGATGTTATGGATGACAGTGACCCCGACTTGGATGAACCACAAATTGAGCACTTGCTGCAAACTGCTGAAGCCATTAGAAAAGACTATCCCAATGAAGATTGGTTGCACTTGACTGCTCTTATTCATGATCTCGGAAAAGTGCTTCTTCTTCCTAGCTTTGGAGAGCTTCCTCAATGGGCTGTTGTTGGAGACACACATCCTCTCGGGTGCGCTTTCGATGAATCCATTGTTCACCACAAGTATTTCAAGGAAAATCCAGATTACAATAATCCTTCCTACAACACCAAAAATGGGATTTACTCAAAAGGATGTGGACTAGACAATGTCATGATCTCATGGGGTCATGATGACTACATGTACTTGGTGGCCAAGGAAAACGGAACAACTTTGCCTCAGGCAGGATTGTTCATTATCCGGTATCACTCATTTTATCCTTTACATAGGGCAGGGGCATATACACACCTTATGAACAAAGAGGACGAAGAGAATCTAAAGTGGCTTCAAGTATTCAACAAATATGACCTCTACAGCAAGAGCAAAGTTCGAGTTGATGTCGAAAAAGTTAAGCCATATTATTTGTCCCTCATTGAGAAGTATTTCCCAGCAAAGCTCAGATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005975 GO:0005996 GO:0006082 GO:0006732 GO:0006766 GO:0006767 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009110 GO:0009987 GO:0016051 GO:0016053 GO:0016491 GO:0016701 GO:0019752 GO:0019852 GO:0019853 GO:0042364 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0046364 GO:0046394 GO:0050113 GO:0051186 GO:0051188 GO:0055114 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00053 ko00562 map00053 map00562

Pfam Domains

Protein Families

MIOX

Protein Analysis

334

Amino Acids

38.7

Weight (kDa)

5.01

Isoelectric Point (pI)

44.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MIOX	PF05153	84 - 333	1.3e-125	Myo-inositol oxygenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001122)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19800 AT4G26260 AT4G26260 AT5G56640 AT5G56640
fragaria_vesca	FvH4_1g06630 FvH4_1g06630 FvH4_5g01690 FvH4_5g20650
malus_domestica	MD02G1069300.v1.1 MD12G1147200.v1.1 MD15G1201000.v1.1
prunus_persica	Prupe.2G089300_v2.0.a1 Prupe.5G054000_v2.0.a1 Prupe.5G054100_v2.0.a1 Prupe.7G216300_v2.0.a1
pyrus_communis	pycom15g17840
rosa_chinensis	RchiOBHm_Chr2g0092361 RchiOBHm_Chr7g0199031 RchiOBHm_Chr7g0206031 RchiOBHm_Chr7g0206141 RchiOBHm_Chr7g0206211
rosa_laevigata	RLG00000003333 RLG00000003336 RLG00000003821 RLG00000003822 RLG00000016303
rosa_multiflora	Rmu_sc0000076.1_g000016 Rmu_sc0003289.1_g000008 Rmu_sc0004084.1_g000023 Rmu_sc0004084.1_g000036 Rmu_sc0007328.1_g000002 Rmu_sc0010737.1_g000001
rosa_roxburghii	Rroxscaffold_2G00149200 Rroxscaffold_3G00251770 Rroxscaffold_3G00251780 Rroxscaffold_3G00251820 Rroxscaffold_3G00257200
rosa_rugosa	Rorug02G0025300.1 Rorug07G0052200 Rorug07G0094600 Rorug07G0094700 Rorug07G0094800
rosa_samantha	Rh2AG071300 Rh7AG175300 Rh7AG226800 Rh7BG178700 Rh7BG223000 Rh7CG184500 Rh7CG240800 Rh7DG178300 Rh7DG232900
rosa_wichuraiana	Rw0G012930 Rw2G006110 Rw7G015380 Rw7G019500 Rw7G019530 Rw7G019580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	228
AccB1I	GGYRCC	1 cut(s)	115
AclWI	GGATC	2 cut(s)	175, 271
AcoI	YGGCCR	2 cut(s)	208, 747
AcsI	RAATTY	1 cut(s)	263
AcuI	CTGAAG	2 cut(s)	48, 468
AfaI	GTAC	2 cut(s)	326, 740
AfiI	CCNNNNNNNGG	5 cut(s)	29, 409, 539, 672, 779
AflIII	ACRYGT	1 cut(s)	735
AgeI	ACCGGT	1 cut(s)	270
AgsI	TTSAA	5 cut(s)	194, 337, 628, 884, 892
AluBI	AGCT	5 cut(s)	28, 373, 537, 547, 991
AluI	AGCT	5 cut(s)	28, 373, 537, 547, 991
Alw21I	GWGCWC	1 cut(s)	432
Alw26I	GTCTC	1 cut(s)	564
AlwI	GGATC	2 cut(s)	175, 271
Ama87I	CYCGRG	1 cut(s)	584
AoxI	GGCC	3 cut(s)	177, 208, 747
ApeKI	GCWGC	3 cut(s)	19, 373, 436
ApoI	RAATTY	1 cut(s)	263
AseI	ATTAAT	1 cut(s)	285
AsiGI	ACCGGT	1 cut(s)	270
Asp700I	GAANNNNTTC	1 cut(s)	977
AspLEI	GCGC	1 cut(s)	593
AsuHPI	GGTGA	1 cut(s)	605
AvaI	CYCGRG	1 cut(s)	584
AxyI	CCTNAGG	1 cut(s)	774
BaeGI	GKGCMC	1 cut(s)	120
BalI	TGGCCA	2 cut(s)	210, 749
BanI	GGYRCC	1 cut(s)	115
Bbv12I	GWGCWC	1 cut(s)	432
BbvI	GCAGC	3 cut(s)	31, 360, 423
BccI	CCATC	3 cut(s)	95, 134, 990
BcoDI	GTCTC	1 cut(s)	564
BfaI	CTAG	3 cut(s)	29, 534, 698
BfmI	CTRYAG	1 cut(s)	907
BglII	AGATCT	1 cut(s)	126
BisI	GCNGC	3 cut(s)	20, 374, 437
BlsI	GCNGC	3 cut(s)	21, 375, 438
BmeT110I	CYCGRG	1 cut(s)	584
BmiI	GGNNCC	1 cut(s)	117
Bpu10I	CCTNAGC	1 cut(s)	24
BsaJI	CCNNGG	2 cut(s)	255, 750
BsaWI	WCCGGW	2 cut(s)	270, 795
Bsc4I	CCNNNNNNNGG	5 cut(s)	29, 409, 539, 672, 779
Bse118I	RCCGGY	1 cut(s)	270
Bse1I	ACTGG	1 cut(s)	211
Bse21I	CCTNAGG	1 cut(s)	774
Bse3DI	GCAATG	1 cut(s)	183
BseDI	CCNNGG	2 cut(s)	255, 750
BseGI	GGATG	5 cut(s)	318, 397, 418, 577, 695
BseLI	CCNNNNNNNGG	5 cut(s)	29, 409, 539, 672, 779
BseMI	GCAATG	1 cut(s)	183
BseMII	CTCAG	2 cut(s)	15, 788
BseNI	ACTGG	1 cut(s)	211
BseRI	GAGGAG	1 cut(s)	335
BseSI	GKGCMC	1 cut(s)	120
BseXI	GCAGC	3 cut(s)	31, 360, 423
BseYI	CCCAGC	1 cut(s)	982
BshFI	GGCC	3 cut(s)	179, 210, 749
BshNI	GGYRCC	1 cut(s)	115
BshTI	ACCGGT	1 cut(s)	270
BsiHKAI	GWGCWC	1 cut(s)	432
BsiHKCI	CYCGRG	1 cut(s)	584
BsiSI	CCGG	2 cut(s)	271, 796
BslFI	GGGAC	1 cut(s)	946
BslI	CCNNNNNNNGG	5 cut(s)	29, 409, 539, 672, 779
BsmAI	GTCTC	1 cut(s)	564
BsmFI	GGGAC	1 cut(s)	946
BsnI	GGCC	3 cut(s)	179, 210, 749
BsoBI	CYCGRG	1 cut(s)	584
Bsp1286I	GDGCHC	2 cut(s)	120, 432
Bsp143I	GATC	5 cut(s)	126, 167, 276, 508, 711
BspANI	GGCC	3 cut(s)	179, 210, 749
BspCNI	CTCAG	2 cut(s)	16, 787
BspHI	TCATGA	3 cut(s)	505, 708, 724
BspLI	GGNNCC	1 cut(s)	117
BspPI	GGATC	2 cut(s)	175, 271
BspT107I	GGYRCC	1 cut(s)	115
BsrDI	GCAATG	1 cut(s)	183
BsrFI	RCCGGY	1 cut(s)	270
BsrI	ACTGG	1 cut(s)	211
BssAI	RCCGGY	1 cut(s)	270
BssECI	CCNNGG	2 cut(s)	255, 750
BssMI	GATC	5 cut(s)	126, 167, 276, 508, 711
BssT1I	CCWWGG	1 cut(s)	750
Bst4CI	ACNGT	2 cut(s)	148, 398
Bst6I	CTCTTC	2 cut(s)	302, 858
BstAPI	GCANNNNNTGC	1 cut(s)	436
BstDEI	CTNAG	3 cut(s)	24, 774, 992
BstDSI	CCRYGG	1 cut(s)	255
BstENI	CCTNNNNNAGG	2 cut(s)	27, 777
BstF5I	GGATG	5 cut(s)	318, 397, 418, 577, 695
BstHHI	GCGC	1 cut(s)	593
BstKTI	GATC	5 cut(s)	129, 170, 279, 511, 714
BstMAI	GTCTC	1 cut(s)	564
BstMBI	GATC	5 cut(s)	126, 167, 276, 508, 711
BstMWI	GCNNNNNNNGC	3 cut(s)	25, 185, 436
BstNSI	RCATGY	1 cut(s)	739
BstSFI	CTRYAG	1 cut(s)	907
BstSLI	GKGCMC	1 cut(s)	120
BstV1I	GCAGC	3 cut(s)	31, 360, 423
BstX2I	RGATCY	2 cut(s)	126, 167
BstYI	RGATCY	2 cut(s)	126, 167
Bsu36I	CCTNAGG	1 cut(s)	774
BsuRI	GGCC	3 cut(s)	179, 210, 749
BtgI	CCRYGG	1 cut(s)	255
BtsCI	GGATG	5 cut(s)	318, 397, 418, 577, 695
BtsIMutI	CAGTG	1 cut(s)	403
CciI	TCATGA	3 cut(s)	505, 708, 724
CfoI	GCGC	1 cut(s)	593
Cfr10I	RCCGGY	1 cut(s)	270
Csp6I	GTAC	2 cut(s)	325, 739
CspAI	ACCGGT	1 cut(s)	270
CviAII	CATG	6 cut(s)	296, 506, 709, 717, 725, 736
CviQI	GTAC	2 cut(s)	325, 739
DdeI	CTNAG	3 cut(s)	24, 774, 992
DpnI	GATC	5 cut(s)	128, 169, 278, 510, 713
DpnII	GATC	5 cut(s)	126, 167, 276, 508, 711
EaeI	YGGCCR	2 cut(s)	208, 747
Eam1104I	CTCTTC	2 cut(s)	302, 858
EarI	CTCTTC	2 cut(s)	302, 858
Eco130I	CCWWGG	1 cut(s)	750
Eco147I	AGGCCT	1 cut(s)	179
Eco57I	CTGAAG	2 cut(s)	48, 468
Eco81I	CCTNAGG	1 cut(s)	774
Eco88I	CYCGRG	1 cut(s)	584
EcoNI	CCTNNNNNAGG	2 cut(s)	27, 777
EcoRI	GAATTC	1 cut(s)	263
EcoT14I	CCWWGG	1 cut(s)	750
ErhI	CCWWGG	1 cut(s)	750
FaeI	CATG	6 cut(s)	299, 509, 712, 720, 728, 739
FalI	AAGNNNNNCTT	2 cut(s)	510, 542
FaqI	GGGAC	1 cut(s)	946
FatI	CATG	6 cut(s)	295, 505, 708, 716, 724, 735
FauNDI	CATATG	1 cut(s)	356
Fnu4HI	GCNGC	3 cut(s)	20, 374, 437
FokI	GGATG	5 cut(s)	325, 404, 425, 564, 702
Fsp4HI	GCNGC	3 cut(s)	20, 374, 437
FspBI	CTAG	3 cut(s)	29, 534, 698
GlaI	GCGC	1 cut(s)	592
GluI	GCNGC	3 cut(s)	20, 374, 437
GsaI	CCCAGC	1 cut(s)	986
HaeIII	GGCC	3 cut(s)	179, 210, 749
HapII	CCGG	2 cut(s)	271, 796
HhaI	GCGC	1 cut(s)	593
Hin1II	CATG	6 cut(s)	299, 509, 712, 720, 728, 739
Hin6I	GCGC	1 cut(s)	591
HinP1I	GCGC	1 cut(s)	591
HinfI	GANTC	3 cut(s)	337, 602, 868
HpaII	CCGG	2 cut(s)	271, 796
HphI	GGTGA	1 cut(s)	605
Hpy166II	GTNNAC	2 cut(s)	613, 695
Hpy188I	TCNGA	3 cut(s)	67, 515, 995
Hpy188III	TCNNGA	5 cut(s)	304, 506, 638, 709, 725
Hpy8I	GTNNAC	2 cut(s)	613, 695
HpyAV	CCTTC	2 cut(s)	190, 663
HpyCH4III	ACNGT	2 cut(s)	148, 398
HpyCH4V	TGCA	4 cut(s)	188, 233, 439, 487
HpyF10VI	GCNNNNNNNGC	3 cut(s)	25, 185, 436
HpyF3I	CTNAG	3 cut(s)	24, 774, 992
Hsp92II	CATG	6 cut(s)	299, 509, 712, 720, 728, 739
HspAI	GCGC	1 cut(s)	591
Kzo9I	GATC	5 cut(s)	126, 167, 276, 508, 711
Lsp1109I	GCAGC	3 cut(s)	31, 360, 423
MaeI	CTAG	3 cut(s)	29, 534, 698
MaeIII	GTNAC	2 cut(s)	33, 398
MalI	GATC	5 cut(s)	128, 169, 278, 510, 713
MboI	GATC	5 cut(s)	126, 167, 276, 508, 711
MboII	GAAGA	7 cut(s)	54, 64, 319, 488, 518, 521, 875
MflI	RGATCY	2 cut(s)	126, 167
MhlI	GDGCHC	2 cut(s)	120, 432
MlsI	TGGCCA	2 cut(s)	210, 749
MluCI	AATT	4 cut(s)	159, 199, 263, 423
MluNI	TGGCCA	2 cut(s)	210, 749
MmeI	TCCRAC	1 cut(s)	547
Mox20I	TGGCCA	2 cut(s)	210, 749
MroXI	GAANNNNTTC	1 cut(s)	977
MscI	TGGCCA	2 cut(s)	210, 749
MseI	TTAA	2 cut(s)	285, 945
Msp20I	TGGCCA	2 cut(s)	210, 749
MspI	CCGG	2 cut(s)	271, 796
MwoI	GCNNNNNNNGC	3 cut(s)	25, 185, 436
NdeI	CATATG	1 cut(s)	356
NdeII	GATC	5 cut(s)	126, 167, 276, 508, 711
NlaIII	CATG	6 cut(s)	299, 509, 712, 720, 728, 739
NlaIV	GGNNCC	1 cut(s)	117
NmuCI	GTSAC	1 cut(s)	398
NspI	RCATGY	1 cut(s)	739
PagI	TCATGA	3 cut(s)	505, 708, 724
PceI	AGGCCT	1 cut(s)	179
PciI	ACATGT	1 cut(s)	735
PdmI	GAANNNNTTC	1 cut(s)	977
PfeI	GAWTC	3 cut(s)	337, 602, 868
PflFI	GACNNNGTC	1 cut(s)	704
PinAI	ACCGGT	1 cut(s)	270
PkrI	GCNGC	3 cut(s)	21, 375, 438
PscI	ACATGT	1 cut(s)	735
PshBI	ATTAAT	1 cut(s)	285
PsiI	TTATAA	1 cut(s)	228
PspFI	CCCAGC	1 cut(s)	982
PspN4I	GGNNCC	1 cut(s)	117
PsuI	RGATCY	2 cut(s)	126, 167
PsyI	GACNNNGTC	1 cut(s)	704
RsaI	GTAC	2 cut(s)	326, 740
RsaNI	GTAC	2 cut(s)	325, 739
SaqAI	TTAA	2 cut(s)	285, 945
SatI	GCNGC	3 cut(s)	20, 374, 437
Sau3AI	GATC	5 cut(s)	126, 167, 276, 508, 711
SduI	GDGCHC	2 cut(s)	120, 432
SetI	ASST	7 cut(s)	30, 375, 539, 549, 846, 906, 993
SfcI	CTRYAG	1 cut(s)	907
Sse9I	AATT	4 cut(s)	159, 199, 263, 423
SseBI	AGGCCT	1 cut(s)	179
SspMI	CTAG	3 cut(s)	29, 534, 698
StuI	AGGCCT	1 cut(s)	179
StyI	CCWWGG	1 cut(s)	750
TaaI	ACNGT	2 cut(s)	148, 398
TaqI	TCGA	4 cut(s)	340, 597, 925, 936
TasI	AATT	4 cut(s)	159, 199, 263, 423
TatI	WGTACW	2 cut(s)	324, 738
TfiI	GAWTC	3 cut(s)	337, 602, 868
Tru1I	TTAA	2 cut(s)	285, 945
Tru9I	TTAA	2 cut(s)	285, 945
TscAI	CASTG	1 cut(s)	403
TseFI	GTSAC	1 cut(s)	398
TseI	GCWGC	3 cut(s)	19, 373, 436
Tsp45I	GTSAC	1 cut(s)	398
TspDTI	ATGAA	9 cut(s)	98, 172, 192, 429, 489, 494, 615, 778, 863
TspGWI	ACGGA	2 cut(s)	244, 774
TspRI	CASTG	1 cut(s)	403
Tth111I	GACNNNGTC	1 cut(s)	704
VspI	ATTAAT	1 cut(s)	285
XagI	CCTNNNNNAGG	2 cut(s)	27, 777
XapI	RAATTY	1 cut(s)	263
XceI	RCATGY	1 cut(s)	739
XmnI	GAANNNNTTC	1 cut(s)	977
XspI	CTAG	3 cut(s)	29, 534, 698

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.