MD02G1074500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002351
Physical Location & Seq
Forward (+)
5970721 .. 5973331
2611 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1074500.v1.1.491

Sequence Viewer

Length: 1599 bp
ATGGCTCTCGATCTAACCGGCTCCACAAATTCCCCAAATACACCAAACATCAAAACCCTAATCGGCAAAAACTACGACCTCAAGCTAACCCAATCAATCCAAAACCTTTTATCCGAAATTCCCAAGCAAAGCCCCAAATGCTCCGACTTTACTGAGGAATTCTACGAATTGATGCAAGCTCGAGTCGACCCGCCTCTCGAATCAACCTGGGTCTACGCGGCATTGGCCTTTCGCAGCCGGAATCATCCGAAAGACGACCCTTTGGACCGAGTATCGGCCACAGAGGACTTGTTTCAGCTGCTGTCGATGTGCTCTGCTTCTTGCGGTTCTTCCAAGAGTGTCGCGATGCTCGCTCCCGTGGTTCGCCAGGTGTACAATGTGTGTGTTGATTTGTTTAAGAGGGACTTGTCATTGAAGAGAGAGAAGAAAGTGATGAGAAAGGTTAGGGATTTTGTGGGAACGATTCTTGGCTATATAAATCTGTCTTCTTTCGAAGATTCGGGTGAGACTGACAGTTTGATTACGCCTTTTTCGGGCTTGATTCGTGTTTGGATGAACTCAAATGAGGGGTTGGAGTCCTTCTTGCCTTTAGTGAGTAGTGACATTTTTGGAGGGCTTAGTGAGGGATTTTGTGATGTGAATTACTTGGCTGGAGTTGTTATTGCGGAGGTGTTTTTGTTGAAACTCTGCTTGGATTTCAAAGTCGGGACTTCGGGGAAGGGGTTGGAGAAGGAGTTAAGCACTTGGGCTATCGGTTCCATTACAACGTTTGGGAACTGTTACCTTTTCGATGTACTTCTGAGGATGCTGCTGGAGAAGACTTTGCCTATTACCTCTCTATTGAGTTCTGAAGGTGAAGTTTTGTTGAGAAAAGTGCTGTATGATGCTATCGTATTGGTCGAGTATTCATTCCTCAACCCTGACCCTGAGAGAGCGATTTACGTACCTGCTGAGCTCATGAAAAGTATTGCCATGGAAAGATTGATTGTTGCTCATGAAGCAGTGGAGTATTTTAGGGAGCACGGGGATCAGAGGAGAGCCATCTCTTATGTAACTGCATTTTCTGGTTCACAGTTACCTTCTCAAATAATTAAATGGGTAAAGAAACAGATTTATGTGGATGATGGTGCAATCAAATCAGTGGGAACTTCGCCTAGAGCTCTTATAAAATGGCTTCTTAACCTTGAGGATCAGGGCTTAAAATTATTTGATGATAGCATTGTGAAGTTTCGTACCAAACTGGCTCTTGATATTTCCAAATCAGACTCCTTGCGGCCTGCTTCTAATTTGGAGGAACAGAAAGCGGATGATGATCTTCTTTTTTACATAGATAACAAGGGAGAAGATGAACATACAGATGAGGAAGATGAAAGGGTGAACGAATCCATTGCTGCCACACATATGATGTCATCATCGGAAAAAAGAGGAAGAAAGCGGAAAGGGAAAAAAGCTGAGAAGAAAGAGAAGATAAAGTTTGTGAAGTATGACCTTCGTCCTAATGCCGACTCAGCCGGTGGAAGGCCCTCACTTATCAACAATGATAGCATAAACAGTGGGAGTGAAGCTGAGAATCCACTTTCTGATGAGGATACAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

533

Amino Acids

59.51

Weight (kDa)

5.16

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1166
Acc36I ACCTGC 1 cut(s) 955
AccI GTMKAC 2 cut(s) 186, 213
AccII CGCG 2 cut(s) 218, 344
AciI CCGC 7 cut(s) 191, 218, 324, 665, 1273, 1304, 1435
AclI AACGTT 1 cut(s) 767
AclWI GGATC 2 cut(s) 1035, 1197
AcoI YGGCCR 1 cut(s) 276
AcsI RAATTY 3 cut(s) 28, 117, 158
AcuI CTGAAG 1 cut(s) 870
AfaI GTAC 4 cut(s) 374, 795, 945, 1234
AfiI CCNNNNNNNGG 3 cut(s) 274, 533, 1518
AgsI TTSAA 3 cut(s) 415, 682, 700
AjnI CCWGG 2 cut(s) 206, 366
AjuI GAANNNNNNNTTGG 2 cut(s) 674, 706
AluBI AGCT 7 cut(s) 85, 179, 298, 955, 1160, 1451, 1565
AluI AGCT 7 cut(s) 85, 179, 298, 955, 1160, 1451, 1565
Alw21I GWGCWC 4 cut(s) 314, 957, 1023, 1162
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 2 cut(s) 1035, 1197
AlwNI CAGNNNCTG 1 cut(s) 301
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 4 cut(s) 225, 276, 1274, 1520
ApeKI GCWGC 4 cut(s) 234, 298, 808, 1391
ApoI RAATTY 3 cut(s) 28, 117, 158
ArsI GACNNNNNNTTYG 2 cut(s) 499, 531
AspS9I GGNCC 2 cut(s) 265, 1521
AsuHPI GGTGA 3 cut(s) 515, 866, 1387
AsuII TTCGAA 1 cut(s) 492
AvaI CYCGRG 1 cut(s) 180
AvaII GGWCC 1 cut(s) 265
BanII GRGCYC 2 cut(s) 957, 1162
BbsI GAAGAC 2 cut(s) 477, 824
Bbv12I GWGCWC 4 cut(s) 314, 957, 1023, 1162
BbvI GCAGC 4 cut(s) 246, 285, 795, 1378
BccI CCATC 2 cut(s) 1049, 1118
BcgI CGANNNNNNTGC 2 cut(s) 1370, 1404
BciT130I CCWGG 2 cut(s) 208, 368
BciVI GTATCC 1 cut(s) 1582
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 1 cut(s) 1155
BfuAI ACCTGC 1 cut(s) 955
BfuI GTATCC 1 cut(s) 1582
BisI GCNGC 6 cut(s) 219, 235, 299, 809, 1274, 1392
BlpI GCTNAGC 1 cut(s) 951
BlsI GCNGC 6 cut(s) 220, 236, 300, 810, 1275, 1393
Bme1390I CCNGG 2 cut(s) 208, 368
Bme18I GGWCC 1 cut(s) 265
BmeT110I CYCGRG 1 cut(s) 180
BmgT120I GGNCC 2 cut(s) 265, 1521
BmiI GGNNCC 2 cut(s) 22, 757
BmrFI CCNGG 2 cut(s) 208, 368
BmsI GCATC 4 cut(s) 162, 336, 795, 874
BoxI GACNNNNGTC 1 cut(s) 1491
BpiI GAAGAC 2 cut(s) 477, 824
BpmI CTGGAG 2 cut(s) 672, 833
Bpu1102I GCTNAGC 1 cut(s) 951
Bpu14I TTCGAA 1 cut(s) 492
BpuEI CTTGAG 2 cut(s) 65, 1205
BsaAI YACGTR 1 cut(s) 943
BsaBI GATNNNNATC 1 cut(s) 1311
BsaJI CCNNGG 3 cut(s) 207, 357, 972
BsaXI ACNNNNNCTCC 2 cut(s) 719, 749
Bsc4I CCNNNNNNNGG 3 cut(s) 274, 533, 1518
Bse118I RCCGGY 2 cut(s) 17, 1511
Bse1I ACTGG 1 cut(s) 1245
Bse3DI GCAATG 1 cut(s) 1386
Bse8I GATNNNNATC 1 cut(s) 1311
BseBI CCWGG 2 cut(s) 208, 368
BseDI CCNNGG 3 cut(s) 207, 357, 972
BseGI GGATG 5 cut(s) 244, 558, 810, 1126, 1312
BseJI GATNNNNATC 1 cut(s) 1311
BseLI CCNNNNNNNGG 3 cut(s) 274, 533, 1518
BseMI GCAATG 1 cut(s) 1386
BseMII CTCAG 7 cut(s) 144, 791, 918, 942, 1443, 1521, 1557
BseNI ACTGG 1 cut(s) 1245
BseRI GAGGAG 1 cut(s) 1048
BseXI GCAGC 4 cut(s) 246, 285, 795, 1378
Bsh1236I CGCG 2 cut(s) 218, 344
BshFI GGCC 4 cut(s) 227, 278, 1276, 1522
BsiHKAI GWGCWC 4 cut(s) 314, 957, 1023, 1162
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 3 cut(s) 18, 238, 1512
BslFI GGGAC 2 cut(s) 416, 721
BslI CCNNNNNNNGG 3 cut(s) 274, 533, 1518
BsmAI GTCTC 1 cut(s) 500
BsmFI GGGAC 2 cut(s) 416, 721
BsnI GGCC 4 cut(s) 227, 278, 1276, 1522
BsoBI CYCGRG 1 cut(s) 180
Bsp119I TTCGAA 1 cut(s) 492
Bsp1286I GDGCHC 4 cut(s) 314, 957, 1023, 1162
Bsp1407I TGTACA 1 cut(s) 372
Bsp143I GATC 4 cut(s) 10, 1027, 1189, 1312
Bsp1720I GCTNAGC 1 cut(s) 951
Bsp19I CCATGG 1 cut(s) 972
Bsp68I TCGCGA 1 cut(s) 344
BspACI CCGC 7 cut(s) 191, 218, 324, 665, 1273, 1304, 1435
BspANI GGCC 4 cut(s) 227, 278, 1276, 1522
BspCNI CTCAG 7 cut(s) 145, 792, 919, 943, 1444, 1520, 1558
BspFNI CGCG 2 cut(s) 218, 344
BspHI TCATGA 2 cut(s) 957, 994
BspLI GGNNCC 2 cut(s) 22, 757
BspMI ACCTGC 1 cut(s) 955
BspPI GGATC 2 cut(s) 1035, 1197
BspT104I TTCGAA 1 cut(s) 492
BsrDI GCAATG 1 cut(s) 1386
BsrFI RCCGGY 2 cut(s) 17, 1511
BsrGI TGTACA 1 cut(s) 372
BsrI ACTGG 1 cut(s) 1245
BssAI RCCGGY 2 cut(s) 17, 1511
BssECI CCNNGG 3 cut(s) 207, 357, 972
BssMI GATC 4 cut(s) 10, 1027, 1189, 1312
BssT1I CCWWGG 1 cut(s) 972
Bst2UI CCWGG 2 cut(s) 208, 368
Bst4CI ACNGT 4 cut(s) 515, 779, 1074, 1553
Bst6I CTCTTC 1 cut(s) 410
BstAUI TGTACA 1 cut(s) 372
BstBAI YACGTR 1 cut(s) 943
BstBI TTCGAA 1 cut(s) 492
BstC8I GCNNGC 3 cut(s) 177, 351, 1278
BstDEI CTNAG 8 cut(s) 153, 617, 800, 927, 951, 1452, 1507, 1566
BstDSI CCRYGG 2 cut(s) 357, 972
BstF5I GGATG 5 cut(s) 244, 558, 810, 1126, 1312
BstFNI CGCG 2 cut(s) 218, 344
BstKTI GATC 4 cut(s) 13, 1030, 1192, 1315
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 4 cut(s) 10, 1027, 1189, 1312
BstMWI GCNNNNNNNGC 5 cut(s) 138, 224, 350, 998, 1508
BstNI CCWGG 2 cut(s) 208, 368
BstPAI GACNNNNGTC 1 cut(s) 1491
BstSCI CCNGG 2 cut(s) 206, 366
BstSNI TACGTA 1 cut(s) 943
BstUI CGCG 2 cut(s) 218, 344
BstV1I GCAGC 4 cut(s) 246, 285, 795, 1378
BstV2I GAAGAC 2 cut(s) 477, 824
BsuI GTATCC 1 cut(s) 1582
BsuRI GGCC 4 cut(s) 227, 278, 1276, 1522
BtgI CCRYGG 2 cut(s) 357, 972
BtgZI GCGATG 1 cut(s) 359
BtsCI GGATG 5 cut(s) 244, 558, 810, 1126, 1312
BtsI GCAGTG 1 cut(s) 1008
BtsIMutI CAGTG 3 cut(s) 1008, 1146, 1558
BtuMI TCGCGA 1 cut(s) 344
BveI ACCTGC 1 cut(s) 955
Cac8I GCNNGC 3 cut(s) 177, 351, 1278
CaiI CAGNNNCTG 1 cut(s) 301
CciI TCATGA 2 cut(s) 957, 994
Cfr10I RCCGGY 2 cut(s) 17, 1511
Cfr13I GGNCC 2 cut(s) 265, 1521
Csp6I GTAC 4 cut(s) 373, 794, 944, 1233
CviAII CATG 3 cut(s) 958, 973, 995
CviQI GTAC 4 cut(s) 373, 794, 944, 1233
DdeI CTNAG 8 cut(s) 153, 617, 800, 927, 951, 1452, 1507, 1566
DpnI GATC 4 cut(s) 12, 1029, 1191, 1314
DpnII GATC 4 cut(s) 10, 1027, 1189, 1312
EaeI YGGCCR 1 cut(s) 276
Eam1104I CTCTTC 1 cut(s) 410
EarI CTCTTC 1 cut(s) 410
Ecl136II GAGCTC 2 cut(s) 955, 1160
Eco105I TACGTA 1 cut(s) 943
Eco130I CCWWGG 1 cut(s) 972
Eco24I GRGCYC 2 cut(s) 957, 1162
Eco47I GGWCC 1 cut(s) 265
Eco53kI GAGCTC 2 cut(s) 955, 1160
Eco57I CTGAAG 1 cut(s) 870
Eco88I CYCGRG 1 cut(s) 180
EcoICRI GAGCTC 2 cut(s) 955, 1160
EcoO109I RGGNCCY 1 cut(s) 1521
EcoRI GAATTC 1 cut(s) 158
EcoRII CCWGG 2 cut(s) 206, 366
EcoT14I CCWWGG 1 cut(s) 972
EcoT38I GRGCYC 2 cut(s) 957, 1162
ErhI CCWWGG 1 cut(s) 972
FaeI CATG 3 cut(s) 961, 976, 998
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FaqI GGGAC 2 cut(s) 416, 721
FatI CATG 3 cut(s) 957, 972, 994
FauI CCCGC 1 cut(s) 198
FauNDI CATATG 1 cut(s) 1401
FblI GTMKAC 2 cut(s) 186, 213
Fnu4HI GCNGC 6 cut(s) 219, 235, 299, 809, 1274, 1392
FokI GGATG 5 cut(s) 231, 565, 817, 1133, 1319
FriOI GRGCYC 2 cut(s) 957, 1162
Fsp4HI GCNGC 6 cut(s) 219, 235, 299, 809, 1274, 1392
FspBI CTAG 1 cut(s) 1155
GluI GCNGC 6 cut(s) 219, 235, 299, 809, 1274, 1392
GsuI CTGGAG 2 cut(s) 672, 833
HaeIII GGCC 4 cut(s) 227, 278, 1276, 1522
HapII CCGG 3 cut(s) 18, 238, 1512
Hin1II CATG 3 cut(s) 961, 976, 998
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HpaII CCGG 3 cut(s) 18, 238, 1512
HphI GGTGA 3 cut(s) 515, 866, 1387
Hpy166II GTNNAC 5 cut(s) 187, 214, 373, 1070, 1378
Hpy188I TCNGA 9 cut(s) 115, 145, 249, 801, 850, 1032, 1264, 1417, 1582
Hpy188III TCNNGA 7 cut(s) 8, 197, 343, 706, 958, 995, 1247
Hpy8I GTNNAC 5 cut(s) 187, 214, 373, 1070, 1378
HpyAV CCTTC 7 cut(s) 589, 712, 724, 845, 1089, 1499, 1512
HpyCH4III ACNGT 4 cut(s) 515, 779, 1074, 1553
HpyCH4IV ACGT 2 cut(s) 767, 942
HpyCH4V TGCA 3 cut(s) 175, 1058, 1130
HpyF10VI GCNNNNNNNGC 5 cut(s) 138, 224, 350, 998, 1508
HpyF3I CTNAG 8 cut(s) 153, 617, 800, 927, 951, 1452, 1507, 1566
HpySE526I ACGT 2 cut(s) 767, 942
Hsp92II CATG 3 cut(s) 961, 976, 998
Kzo9I GATC 4 cut(s) 10, 1027, 1189, 1312
LmnI GCTCC 4 cut(s) 26, 146, 358, 1018
Lsp1109I GCAGC 4 cut(s) 246, 285, 795, 1378
LweI GCATC 4 cut(s) 162, 336, 795, 874
MaeI CTAG 1 cut(s) 1155
MaeII ACGT 2 cut(s) 767, 942
MaeIII GTNAC 4 cut(s) 599, 779, 1051, 1074
MalI GATC 4 cut(s) 12, 1029, 1191, 1314
MboI GATC 4 cut(s) 10, 1027, 1189, 1312
MhlI GDGCHC 4 cut(s) 314, 957, 1023, 1162
MluCI AATT 8 cut(s) 28, 117, 158, 167, 640, 1089, 1202, 1285
MlyI GAGTC 4 cut(s) 192, 584, 1259, 1499
MmeI TCCRAC 3 cut(s) 168, 552, 705
MseI TTAA 5 cut(s) 396, 737, 1092, 1179, 1199
MslI CAYNNNNRTG 2 cut(s) 1356, 1400
MspA1I CMGCKG 1 cut(s) 298
MspI CCGG 3 cut(s) 18, 238, 1512
MspR9I CCNGG 2 cut(s) 208, 368
MvaI CCWGG 2 cut(s) 208, 368
MvnI CGCG 2 cut(s) 218, 344
MwoI GCNNNNNNNGC 5 cut(s) 138, 224, 350, 998, 1508
NcoI CCATGG 1 cut(s) 972
NdeI CATATG 1 cut(s) 1401
NdeII GATC 4 cut(s) 10, 1027, 1189, 1312
NlaIII CATG 3 cut(s) 961, 976, 998
NlaIV GGNNCC 2 cut(s) 22, 757
NmuCI GTSAC 1 cut(s) 599
NruI TCGCGA 1 cut(s) 344
NspV TTCGAA 1 cut(s) 492
PaeR7I CTCGAG 1 cut(s) 180
PagI TCATGA 2 cut(s) 957, 994
PcsI WCGNNNNNNNCGW 1 cut(s) 897
PfeI GAWTC 7 cut(s) 200, 241, 463, 497, 541, 1382, 1570
PkrI GCNGC 6 cut(s) 220, 236, 300, 810, 1275, 1393
PleI GAGTC 4 cut(s) 191, 583, 1259, 1499
PpsI GAGTC 4 cut(s) 191, 583, 1259, 1499
Ppu21I YACGTR 1 cut(s) 943
PshAI GACNNNNGTC 1 cut(s) 1491
PsiI TTATAA 1 cut(s) 1166
Psp124BI GAGCTC 2 cut(s) 957, 1162
Psp1406I AACGTT 1 cut(s) 767
Psp6I CCWGG 2 cut(s) 206, 366
PspGI CCWGG 2 cut(s) 206, 366
PspN4I GGNNCC 2 cut(s) 22, 757
PspPI GGNCC 2 cut(s) 265, 1521
PspXI VCTCGAGB 1 cut(s) 180
PstNI CAGNNNCTG 1 cut(s) 301
PvuII CAGCTG 1 cut(s) 298
RruI TCGCGA 1 cut(s) 344
RsaI GTAC 4 cut(s) 374, 795, 945, 1234
RsaNI GTAC 4 cut(s) 373, 794, 944, 1233
RseI CAYNNNNRTG 2 cut(s) 1356, 1400
SacI GAGCTC 2 cut(s) 957, 1162
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 5 cut(s) 396, 737, 1092, 1179, 1199
SatI GCNGC 6 cut(s) 219, 235, 299, 809, 1274, 1392
Sau3AI GATC 4 cut(s) 10, 1027, 1189, 1312
Sau96I GGNCC 2 cut(s) 265, 1521
SchI GAGTC 4 cut(s) 192, 584, 1259, 1499
ScrFI CCNGG 2 cut(s) 208, 368
SduI GDGCHC 4 cut(s) 314, 957, 1023, 1162
SfaNI GCATC 4 cut(s) 162, 336, 795, 874
Sfr274I CTCGAG 1 cut(s) 180
SfuI TTCGAA 1 cut(s) 492
SinI GGWCC 1 cut(s) 265
SlaI CTCGAG 1 cut(s) 180
SmiMI CAYNNNNRTG 2 cut(s) 1356, 1400
SmlI CTYRAG 3 cut(s) 80, 180, 1184
SmoI CTYRAG 3 cut(s) 80, 180, 1184
SnaBI TACGTA 1 cut(s) 943
Sse9I AATT 8 cut(s) 28, 117, 158, 167, 640, 1089, 1202, 1285
SsiI CCGC 7 cut(s) 191, 218, 324, 665, 1273, 1304, 1435
SspMI CTAG 1 cut(s) 1155
SstI GAGCTC 2 cut(s) 957, 1162
StyD4I CCNGG 2 cut(s) 206, 366
StyI CCWWGG 1 cut(s) 972
TaaI ACNGT 4 cut(s) 515, 779, 1074, 1553
TaiI ACGT 2 cut(s) 770, 945
TaqI TCGA 8 cut(s) 9, 181, 186, 198, 305, 492, 789, 900
TaqII GACCGA 1 cut(s) 282
TasI AATT 8 cut(s) 28, 117, 158, 167, 640, 1089, 1202, 1285
TatI WGTACW 2 cut(s) 372, 793
TauI GCSGC 2 cut(s) 221, 1276
TfiI GAWTC 7 cut(s) 200, 241, 463, 497, 541, 1382, 1570
Tru1I TTAA 5 cut(s) 396, 737, 1092, 1179, 1199
Tru9I TTAA 5 cut(s) 396, 737, 1092, 1179, 1199
TscAI CASTG 3 cut(s) 1008, 1146, 1558
TseFI GTSAC 1 cut(s) 599
TseI GCWGC 4 cut(s) 234, 298, 808, 1391
Tsp45I GTSAC 1 cut(s) 599
TspDTI ATGAA 6 cut(s) 569, 897, 974, 1011, 1362, 1383
TspRI CASTG 3 cut(s) 1008, 1146, 1558
VpaK11BI GGWCC 1 cut(s) 265
XapI RAATTY 3 cut(s) 28, 117, 158
XhoI CTCGAG 1 cut(s) 180
XmiI GTMKAC 2 cut(s) 186, 213
XspI CTAG 1 cut(s) 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.