pycom02g05890

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002351
Physical Location & Seq
Forward (+)
3972602 .. 3974732
2131 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g05890.4

Sequence Viewer

Length: 1599 bp
ATGGCTCTCGATCTAACCGGCTCCACAAATTCCCCAAATACAGCAAACATCCAAACCCTAATCGGCAAAAACTACGACCTCAAGCTAACCCAATCAATCCAAAACCTTTTATCCGAAATTCCCAAGCAAAGCCCCAAACTCTCCGACTTTACAGAGGAATTCTACGAATTGATGCAAGCTCGAGTCGACCCGCCTCTCGAATCAACCTGGGTCTACGCGGCATTGGCCTTTCGCAGCCGGAATCATCCGAAAGACGACCCTTTGGACCGTGTAACGGCCGCAGAGGACTTGTTTCAGCTGCTGTCGATGTGCTCAGCTTCTTGCGGTTCTTCAAAGAGTGTCGCGCTGCTCGCTCCGGTGGTTTGCCAGGTGTACAATGCGTGTGTTGATTTGTTTAAGAGGGACTTGTCATTGAAGAGAGAGAAGAAAGTGATGAGAAAGGTTAGGGATTTTGTGGGAACGATTCTTGGCTATATAAATCTGTGTTCTTTCGAAGATTCGGGTGAGAGTGACAGTTTGATTACGCCTTTTTATGGCTTGATTCGTGTTTGGATGAACTCAAATGAGGGGTTGGAGTCATTCTTGCCTTTAGTGAGTAGTGACATTTTTGGAGGGCTTAGTGAGGGAGAATGTGATGTGAGTTACTTGGCTGGAGTTGTTATTGCGGGGGTGTTTTTGTTGAAACTCTGCTTGGATTTCAAAGTCGGGACTTCGGGGAAGGAGTTGGAGAAGGAGTTAAGCACTTGGGCTATCGGTTCCATTACAAAGTTTGGGAACTGTTACCTTTTCGACGTCCTTCTGAGGATGCTGCTGGAGAAGACTTTGCCTTTTACCTCTCTATTGAGTTCTGAAGGTGAAGTTTTGTTGAGAAAAGTACTGTATGATGCTATCGTATTGGTCGAGTATTCATTCCTCAACCCTGACCCTGAGAGAGCGATTTACGTACCTGCTGAGCTCATGAAAAGTATTGCCATGGAAAGATTGATTGTTGCTCATGAAGCAGTGGAGTATTTTAGGGAGAACGGGGATCAGAGGAGAGCCGTCTCTTTTGTAACTGCATTTTCTGGTTCACAGTTACCTTCTCAAATAATTAAATGGGTAAAGAAACAGATTTATGTGGATGATGGTGCAATCAAATCATTGGGAACTTCGCCTAAAGCTCTTATAAAATGGCTTCTTAACCTTGAGGATCGGGGCTTAAATTTATTTGATGATAGCATTGCGAAGTTTCGTACCAAACTGGCTCTTGATATTTCTAAATCAGACACCTTGCGGCCTGCTTCTAATTTGGAGGAACAGAAAGTGGATGATGATCTTCTTTTTTACATAGATAACAAGGGAGAAGATGAACATAGAGATGAGGAAGATGAAAGGGTGAACGAATCCATAGCTGCCACGCATACAATGCCATCATCAGAAAAAAGAGGAAGAAAGCGGAAAGGGAAAAAAGCTGAGAAGAAAGAGAAGATAAAGTTTGTGAAGTATGACCTTCGTCCTAATGCCGACTCAGCTGGTGGGAGGCCCTCACTTATCAACAATGATAGCATAAACAGTGGGAGTGAAGCTGAGAATCCACCTTCTGATGAGGATACAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

533

Amino Acids

59.45

Weight (kDa)

5.08

Isoelectric Point (pI)

37.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1166
AatII GACGTC 1 cut(s) 795
Acc36I ACCTGC 1 cut(s) 955
AccI GTMKAC 2 cut(s) 186, 213
AccII CGCG 2 cut(s) 218, 344
AciI CCGC 7 cut(s) 191, 218, 279, 324, 665, 1273, 1435
AclWI GGATC 2 cut(s) 1035, 1197
AcoI YGGCCR 1 cut(s) 276
AcsI RAATTY 4 cut(s) 28, 117, 158, 1201
AcuI CTGAAG 1 cut(s) 870
AcyI GRCGYC 1 cut(s) 792
AfaI GTAC 4 cut(s) 374, 876, 945, 1234
AfiI CCNNNNNNNGG 2 cut(s) 274, 533
AgsI TTSAA 4 cut(s) 333, 415, 682, 700
AjnI CCWGG 2 cut(s) 206, 366
AjuI GAANNNNNNNTTGG 2 cut(s) 674, 706
Alw21I GWGCWC 2 cut(s) 314, 957
Alw26I GTCTC 1 cut(s) 1048
AlwI GGATC 2 cut(s) 1035, 1197
AlwNI CAGNNNCTG 1 cut(s) 301
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 4 cut(s) 225, 276, 1274, 1520
ApeKI GCWGC 5 cut(s) 234, 298, 346, 808, 1391
ApoI RAATTY 4 cut(s) 28, 117, 158, 1201
AspLEI GCGC 1 cut(s) 346
AspS9I GGNCC 2 cut(s) 265, 1521
AsuHPI GGTGA 3 cut(s) 515, 866, 1387
AsuII TTCGAA 1 cut(s) 492
AvaI CYCGRG 1 cut(s) 180
AvaII GGWCC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 957
BbsI GAAGAC 1 cut(s) 824
Bbv12I GWGCWC 2 cut(s) 314, 957
BbvI GCAGC 5 cut(s) 246, 285, 333, 795, 1378
BccI CCATC 2 cut(s) 1118, 1417
BceAI ACGGC 2 cut(s) 291, 1025
BciT130I CCWGG 2 cut(s) 208, 368
BciVI GTATCC 1 cut(s) 1582
BcoDI GTCTC 1 cut(s) 1048
BfuAI ACCTGC 1 cut(s) 955
BfuI GTATCC 1 cut(s) 1582
BisI GCNGC 8 cut(s) 219, 235, 279, 299, 347, 809, 1274, 1392
BlpI GCTNAGC 2 cut(s) 313, 951
BlsI GCNGC 8 cut(s) 220, 236, 280, 300, 348, 810, 1275, 1393
BmcAI AGTACT 1 cut(s) 876
Bme1390I CCNGG 2 cut(s) 208, 368
Bme18I GGWCC 1 cut(s) 265
BmeT110I CYCGRG 1 cut(s) 180
BmgT120I GGNCC 2 cut(s) 265, 1521
BmiI GGNNCC 2 cut(s) 22, 757
BmrFI CCNGG 2 cut(s) 208, 368
BmsI GCATC 3 cut(s) 162, 795, 874
BoxI GACNNNNGTC 1 cut(s) 1491
BpiI GAAGAC 1 cut(s) 824
BpmI CTGGAG 2 cut(s) 672, 833
Bpu1102I GCTNAGC 2 cut(s) 313, 951
Bpu14I TTCGAA 1 cut(s) 492
BpuEI CTTGAG 2 cut(s) 65, 1205
BsaAI YACGTR 1 cut(s) 943
BsaBI GATNNNNATC 1 cut(s) 1311
BsaHI GRCGYC 1 cut(s) 792
BsaJI CCNNGG 2 cut(s) 207, 972
BsaWI WCCGGW 1 cut(s) 355
BsaXI ACNNNNNCTCC 2 cut(s) 719, 749
Bsc4I CCNNNNNNNGG 2 cut(s) 274, 533
Bse118I RCCGGY 1 cut(s) 17
Bse1I ACTGG 1 cut(s) 1245
Bse3DI GCAATG 1 cut(s) 1218
Bse8I GATNNNNATC 1 cut(s) 1311
BseBI CCWGG 2 cut(s) 208, 368
BseDI CCNNGG 2 cut(s) 207, 972
BseGI GGATG 6 cut(s) 48, 244, 558, 810, 1126, 1312
BseJI GATNNNNATC 1 cut(s) 1311
BseLI CCNNNNNNNGG 2 cut(s) 274, 533
BseMI GCAATG 1 cut(s) 1218
BseMII CTCAG 7 cut(s) 327, 791, 918, 942, 1443, 1521, 1557
BseNI ACTGG 1 cut(s) 1245
BseRI GAGGAG 1 cut(s) 1048
BseX3I CGGCCG 1 cut(s) 276
BseXI GCAGC 5 cut(s) 246, 285, 333, 795, 1378
Bsh1236I CGCG 2 cut(s) 218, 344
Bsh1285I CGRYCG 1 cut(s) 279
BshFI GGCC 4 cut(s) 227, 278, 1276, 1522
BsiEI CGRYCG 1 cut(s) 279
BsiHKAI GWGCWC 2 cut(s) 314, 957
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 3 cut(s) 18, 238, 356
BslFI GGGAC 2 cut(s) 416, 721
BslI CCNNNNNNNGG 2 cut(s) 274, 533
BsmAI GTCTC 1 cut(s) 1048
BsmBI CGTCTC 1 cut(s) 1048
BsmFI GGGAC 2 cut(s) 416, 721
BsnI GGCC 4 cut(s) 227, 278, 1276, 1522
BsoBI CYCGRG 1 cut(s) 180
Bsp119I TTCGAA 1 cut(s) 492
Bsp1286I GDGCHC 2 cut(s) 314, 957
Bsp1407I TGTACA 1 cut(s) 372
Bsp143I GATC 4 cut(s) 10, 1027, 1189, 1312
Bsp1720I GCTNAGC 2 cut(s) 313, 951
Bsp19I CCATGG 1 cut(s) 972
BspACI CCGC 7 cut(s) 191, 218, 279, 324, 665, 1273, 1435
BspANI GGCC 4 cut(s) 227, 278, 1276, 1522
BspCNI CTCAG 7 cut(s) 326, 792, 919, 943, 1444, 1520, 1558
BspFNI CGCG 2 cut(s) 218, 344
BspHI TCATGA 2 cut(s) 957, 994
BspLI GGNNCC 2 cut(s) 22, 757
BspMI ACCTGC 1 cut(s) 955
BspPI GGATC 2 cut(s) 1035, 1197
BspT104I TTCGAA 1 cut(s) 492
BsrDI GCAATG 1 cut(s) 1218
BsrFI RCCGGY 1 cut(s) 17
BsrGI TGTACA 1 cut(s) 372
BsrI ACTGG 1 cut(s) 1245
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 2 cut(s) 207, 972
BssMI GATC 4 cut(s) 10, 1027, 1189, 1312
BssNI GRCGYC 1 cut(s) 792
BssT1I CCWWGG 1 cut(s) 972
Bst2UI CCWGG 2 cut(s) 208, 368
Bst4CI ACNGT 6 cut(s) 269, 515, 779, 879, 1074, 1553
Bst6I CTCTTC 1 cut(s) 410
BstACI GRCGYC 1 cut(s) 792
BstAPI GCANNNNNTGC 1 cut(s) 1405
BstAUI TGTACA 1 cut(s) 372
BstBAI YACGTR 1 cut(s) 943
BstBI TTCGAA 1 cut(s) 492
BstC8I GCNNGC 3 cut(s) 177, 351, 1278
BstDEI CTNAG 8 cut(s) 313, 617, 800, 927, 951, 1452, 1507, 1566
BstDSI CCRYGG 1 cut(s) 972
BstF5I GGATG 6 cut(s) 48, 244, 558, 810, 1126, 1312
BstFNI CGCG 2 cut(s) 218, 344
BstHHI GCGC 1 cut(s) 346
BstKTI GATC 4 cut(s) 13, 1030, 1192, 1315
BstMAI GTCTC 1 cut(s) 1048
BstMBI GATC 4 cut(s) 10, 1027, 1189, 1312
BstMCI CGRYCG 1 cut(s) 279
BstMWI GCNNNNNNNGC 5 cut(s) 224, 350, 998, 1405, 1508
BstNI CCWGG 2 cut(s) 208, 368
BstPAI GACNNNNGTC 1 cut(s) 1491
BstSCI CCNGG 2 cut(s) 206, 366
BstSNI TACGTA 1 cut(s) 943
BstUI CGCG 2 cut(s) 218, 344
BstV1I GCAGC 5 cut(s) 246, 285, 333, 795, 1378
BstV2I GAAGAC 1 cut(s) 824
BstZI CGGCCG 1 cut(s) 276
BsuI GTATCC 1 cut(s) 1582
BsuRI GGCC 4 cut(s) 227, 278, 1276, 1522
BtgI CCRYGG 1 cut(s) 972
BtsCI GGATG 6 cut(s) 48, 244, 558, 810, 1126, 1312
BtsI GCAGTG 1 cut(s) 1008
BtsIMutI CAGTG 2 cut(s) 1008, 1558
BveI ACCTGC 1 cut(s) 955
Cac8I GCNNGC 3 cut(s) 177, 351, 1278
CaiI CAGNNNCTG 1 cut(s) 301
CciI TCATGA 2 cut(s) 957, 994
CfoI GCGC 1 cut(s) 346
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 2 cut(s) 265, 1521
Csp6I GTAC 4 cut(s) 373, 875, 944, 1233
CviAII CATG 3 cut(s) 958, 973, 995
CviQI GTAC 4 cut(s) 373, 875, 944, 1233
DdeI CTNAG 8 cut(s) 313, 617, 800, 927, 951, 1452, 1507, 1566
DpnI GATC 4 cut(s) 12, 1029, 1191, 1314
DpnII GATC 4 cut(s) 10, 1027, 1189, 1312
EaeI YGGCCR 1 cut(s) 276
EagI CGGCCG 1 cut(s) 276
Eam1104I CTCTTC 1 cut(s) 410
EarI CTCTTC 1 cut(s) 410
Ecl136II GAGCTC 1 cut(s) 955
EclXI CGGCCG 1 cut(s) 276
Eco105I TACGTA 1 cut(s) 943
Eco130I CCWWGG 1 cut(s) 972
Eco24I GRGCYC 1 cut(s) 957
Eco47I GGWCC 1 cut(s) 265
Eco52I CGGCCG 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 955
Eco57I CTGAAG 1 cut(s) 870
Eco88I CYCGRG 1 cut(s) 180
EcoICRI GAGCTC 1 cut(s) 955
EcoO109I RGGNCCY 1 cut(s) 1521
EcoRI GAATTC 1 cut(s) 158
EcoRII CCWGG 2 cut(s) 206, 366
EcoT14I CCWWGG 1 cut(s) 972
EcoT38I GRGCYC 1 cut(s) 957
ErhI CCWWGG 1 cut(s) 972
Esp3I CGTCTC 1 cut(s) 1048
FaeI CATG 3 cut(s) 961, 976, 998
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FaqI GGGAC 2 cut(s) 416, 721
FatI CATG 3 cut(s) 957, 972, 994
FauI CCCGC 2 cut(s) 198, 658
FblI GTMKAC 2 cut(s) 186, 213
Fnu4HI GCNGC 8 cut(s) 219, 235, 279, 299, 347, 809, 1274, 1392
FokI GGATG 6 cut(s) 35, 231, 565, 817, 1133, 1319
FriOI GRGCYC 1 cut(s) 957
Fsp4HI GCNGC 8 cut(s) 219, 235, 279, 299, 347, 809, 1274, 1392
GlaI GCGC 1 cut(s) 345
GluI GCNGC 8 cut(s) 219, 235, 279, 299, 347, 809, 1274, 1392
GsuI CTGGAG 2 cut(s) 672, 833
HaeIII GGCC 4 cut(s) 227, 278, 1276, 1522
HapII CCGG 3 cut(s) 18, 238, 356
HhaI GCGC 1 cut(s) 346
Hin1I GRCGYC 1 cut(s) 792
Hin1II CATG 3 cut(s) 961, 976, 998
Hin6I GCGC 1 cut(s) 344
HinP1I GCGC 1 cut(s) 344
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HpaII CCGG 3 cut(s) 18, 238, 356
HphI GGTGA 3 cut(s) 515, 866, 1387
Hpy166II GTNNAC 5 cut(s) 187, 214, 373, 1070, 1378
Hpy188I TCNGA 9 cut(s) 115, 145, 249, 801, 850, 1032, 1264, 1417, 1582
Hpy188III TCNNGA 6 cut(s) 8, 197, 706, 958, 995, 1247
Hpy8I GTNNAC 5 cut(s) 187, 214, 373, 1070, 1378
Hpy99I CGWCG 1 cut(s) 794
HpyAV CCTTC 7 cut(s) 712, 724, 806, 845, 1089, 1499, 1587
HpyCH4III ACNGT 6 cut(s) 269, 515, 779, 879, 1074, 1553
HpyCH4IV ACGT 2 cut(s) 792, 942
HpyCH4V TGCA 3 cut(s) 175, 1058, 1130
HpyF10VI GCNNNNNNNGC 5 cut(s) 224, 350, 998, 1405, 1508
HpyF3I CTNAG 8 cut(s) 313, 617, 800, 927, 951, 1452, 1507, 1566
HpySE526I ACGT 2 cut(s) 792, 942
Hsp92I GRCGYC 1 cut(s) 792
Hsp92II CATG 3 cut(s) 961, 976, 998
HspAI GCGC 1 cut(s) 344
Kzo9I GATC 4 cut(s) 10, 1027, 1189, 1312
LmnI GCTCC 2 cut(s) 26, 358
Lsp1109I GCAGC 5 cut(s) 246, 285, 333, 795, 1378
LweI GCATC 3 cut(s) 162, 795, 874
MaeII ACGT 2 cut(s) 792, 942
MaeIII GTNAC 7 cut(s) 271, 509, 599, 641, 779, 1051, 1074
MalI GATC 4 cut(s) 12, 1029, 1191, 1314
MboI GATC 4 cut(s) 10, 1027, 1189, 1312
MhlI GDGCHC 2 cut(s) 314, 957
MluCI AATT 7 cut(s) 28, 117, 158, 167, 1089, 1201, 1285
MlyI GAGTC 3 cut(s) 192, 584, 1499
MmeI TCCRAC 3 cut(s) 168, 552, 705
MseI TTAA 5 cut(s) 396, 737, 1092, 1179, 1199
MslI CAYNNNNRTG 1 cut(s) 1356
MspA1I CMGCKG 2 cut(s) 298, 1511
MspI CCGG 3 cut(s) 18, 238, 356
MspR9I CCNGG 2 cut(s) 208, 368
MvaI CCWGG 2 cut(s) 208, 368
MvnI CGCG 2 cut(s) 218, 344
MwoI GCNNNNNNNGC 5 cut(s) 224, 350, 998, 1405, 1508
NcoI CCATGG 1 cut(s) 972
NdeII GATC 4 cut(s) 10, 1027, 1189, 1312
NlaIII CATG 3 cut(s) 961, 976, 998
NlaIV GGNNCC 2 cut(s) 22, 757
NmuCI GTSAC 2 cut(s) 509, 599
NspV TTCGAA 1 cut(s) 492
PaeR7I CTCGAG 1 cut(s) 180
PagI TCATGA 2 cut(s) 957, 994
PcsI WCGNNNNNNNCGW 1 cut(s) 897
PfeI GAWTC 7 cut(s) 200, 241, 463, 497, 541, 1382, 1570
PkrI GCNGC 8 cut(s) 220, 236, 280, 300, 348, 810, 1275, 1393
PleI GAGTC 3 cut(s) 191, 583, 1499
PpsI GAGTC 3 cut(s) 191, 583, 1499
Ppu21I YACGTR 1 cut(s) 943
PshAI GACNNNNGTC 1 cut(s) 1491
PsiI TTATAA 1 cut(s) 1166
Psp124BI GAGCTC 1 cut(s) 957
Psp6I CCWGG 2 cut(s) 206, 366
PspGI CCWGG 2 cut(s) 206, 366
PspN4I GGNNCC 2 cut(s) 22, 757
PspPI GGNCC 2 cut(s) 265, 1521
PspXI VCTCGAGB 1 cut(s) 180
PstNI CAGNNNCTG 1 cut(s) 301
PvuII CAGCTG 2 cut(s) 298, 1511
RsaI GTAC 4 cut(s) 374, 876, 945, 1234
RsaNI GTAC 4 cut(s) 373, 875, 944, 1233
RseI CAYNNNNRTG 1 cut(s) 1356
SacI GAGCTC 1 cut(s) 957
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 5 cut(s) 396, 737, 1092, 1179, 1199
SatI GCNGC 8 cut(s) 219, 235, 279, 299, 347, 809, 1274, 1392
Sau3AI GATC 4 cut(s) 10, 1027, 1189, 1312
Sau96I GGNCC 2 cut(s) 265, 1521
ScaI AGTACT 1 cut(s) 876
SchI GAGTC 3 cut(s) 192, 584, 1499
ScrFI CCNGG 2 cut(s) 208, 368
SduI GDGCHC 2 cut(s) 314, 957
SfaNI GCATC 3 cut(s) 162, 795, 874
Sfr274I CTCGAG 1 cut(s) 180
SfuI TTCGAA 1 cut(s) 492
SinI GGWCC 1 cut(s) 265
SlaI CTCGAG 1 cut(s) 180
SmiMI CAYNNNNRTG 1 cut(s) 1356
SmlI CTYRAG 3 cut(s) 80, 180, 1184
SmoI CTYRAG 3 cut(s) 80, 180, 1184
SnaBI TACGTA 1 cut(s) 943
Sse9I AATT 7 cut(s) 28, 117, 158, 167, 1089, 1201, 1285
SsiI CCGC 7 cut(s) 191, 218, 279, 324, 665, 1273, 1435
SstI GAGCTC 1 cut(s) 957
StyD4I CCNGG 2 cut(s) 206, 366
StyI CCWWGG 1 cut(s) 972
TaaI ACNGT 6 cut(s) 269, 515, 779, 879, 1074, 1553
TaiI ACGT 2 cut(s) 795, 945
TaqI TCGA 8 cut(s) 9, 181, 186, 198, 305, 492, 789, 900
TasI AATT 7 cut(s) 28, 117, 158, 167, 1089, 1201, 1285
TatI WGTACW 2 cut(s) 372, 874
TauI GCSGC 3 cut(s) 221, 281, 1276
TfiI GAWTC 7 cut(s) 200, 241, 463, 497, 541, 1382, 1570
Tru1I TTAA 5 cut(s) 396, 737, 1092, 1179, 1199
Tru9I TTAA 5 cut(s) 396, 737, 1092, 1179, 1199
TscAI CASTG 2 cut(s) 1008, 1558
TseFI GTSAC 2 cut(s) 509, 599
TseI GCWGC 5 cut(s) 234, 298, 346, 808, 1391
Tsp45I GTSAC 2 cut(s) 509, 599
TspDTI ATGAA 6 cut(s) 569, 897, 974, 1011, 1362, 1383
TspRI CASTG 2 cut(s) 1008, 1558
VpaK11BI GGWCC 1 cut(s) 265
XapI RAATTY 4 cut(s) 28, 117, 158, 1201
XhoI CTCGAG 1 cut(s) 180
XmiI GTMKAC 2 cut(s) 186, 213
ZraI GACGTC 1 cut(s) 793
ZrmI AGTACT 1 cut(s) 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.