MD02G1300300.v1.1

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002251
Physical Location & Seq
Forward (+)
35505494 .. 35509564
4071 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1300300.v1.1.491

Sequence Viewer

Length: 1749 bp
ATGGGTGCCGCAGAGCCTGTGGAGGAGAGAGAAAGAGAGGAGCTTGAGGAGAGAGAGGAAATTGGGAGGAAGCAGGAGGCGGAGCAGGGGGTGAGAGATGTTGAGCAAGGGGAGATGGGTTTTGAAGAACAGGCGGTGGGAGCTCAGAGAAATATTAATATTAATCATGGAAATAATAATATTAATCAGAACGAGAGAGAAGGAGATGTTCATGTGTCAATGCTGCAGAGGCTGAATCCAACAAACCCTTTGAGAATTGTAATAAATAGCAAAACCAGAACTGCAACTCCGCCACCGCCGCCGTCACGGATCTCTAATATCAGAGGCGGTGCTACTGCTACTCCTACTCCTCCTTCTCAGTTCTCCTCTCACACTAACAGTCCCCAGCATTCTCAGCCTCGTTCTACACCAACCCCACAACCATCCATCACAACACTGAATTCCAGGAAATTCACCAACAAGATATGTTTGTTCCTGTTTATTTTTCACACGGTTGCCGCCATTGGGCTAGTCGGTTTTCTTCTATTCCGGGGAGTTAAGGGGCTGATAGAATCATCAGACAACAAGGTTAAAAGAGCCGAGAAACGAGTGTTGAAGTTTTTTCTACCACAAGTCGAAGCCGCATCTTTTTTAAGCATTACTCTTGCATTTGCATGGCAAAAGGCAGTGAGGCTATGGCCGATGTTTTTTGTTCACTTCATACTATGGACCACTTTTCTCATGTCTCTATCTGCCGGAATTCTGCTAATTTGCTTCCAAAAGCCTCCCACCAATGGCGTCGGAGTCTGTTTTATTGCCTTCGCAATCGGCAACGGCTTATATGCTTGTTGGATCACACATCGGATTGGATTTTGTAGCAAGATATTGATCAAATCGCTAGAACCAGTATCAAAGTTCCCCGATTTGAATCAACCGACTTATTGGATGCTTGGGGTCGGATTTCTGTGGATGTCCCTGTGGAATCTAGCTGTCATTGGAGCCCTGAATTTCTACTTTCCTCCATTGATCATAATTTTATTGGTCTTGAGCTTAGCTTGGACAACTGAAGTAATGAGGAACGTTGCAAATATAACCGTCAGTAGGGTAATTTCTCTGTATTATCTCAGAGGAATGCAGTCTAATACTCAGTTTTGCTTCCAAAGAGCCTTGAGTAAAAATCTTGGAAGTGCTTGTTTTGGATCTCTCTTTGTTCCTGCAATTGAAGCCCTGAGAATTGTTGCTCGGGGTTTAAATTTACTCGAGGGGGAAGATGAGTTCATGTTTTCTTGTGCACATTGCTGTCTCAGAGTCATGGAGGGCATCTTCCGATATGGCAACGGCTGGGCCTTCGTGCAGATAGCAGCCTACGGGAAGGGCTTCGTGAGGGCGTCGCAAGACACTTGGGAGCTCTTTGAACAGCAAGAAATGGAACAAATTGTTGATTCCGACATTACCAGCTCAATCTGCTTCCTAACGGGCGTCTGCAGTGGCTCTATCTGTGTCATAGTTGTGGCGGCTTGGACCGCTAGAGTGCACCAAAGTTTCACAGCCGCCATTTCCCTCCTTACATTCTTCATTGGATACCTTATGACGAGGATAGCCATGGCACTACCACATGCTTGTGTTGGTAGTTACTTTGTTTGCTACGCAGAGAATCCAGACAACAGATTGTTCGACAACACAATCAAGGATCGCCTCGCTTTGATGAATTCCGGTCGAGATGTAGCCGTTCCCACACCTCGAGTCCCCCGCCGGTTTGCAGCAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

583

Amino Acids

65.1

Weight (kDa)

8.85

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 263 - 547 1e-40 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 5
AclI AACGTT 1 cut(s) 1059
AclWI GGATC 4 cut(s) 317, 839, 1186, 1677
AcoI YGGCCR 1 cut(s) 677
AcsI RAATTY 6 cut(s) 439, 449, 738, 985, 1231, 1687
AcuI CTGAAG 1 cut(s) 1065
AcyI GRCGYC 3 cut(s) 777, 1367, 1458
AfiI CCNNNNNNNGG 3 cut(s) 504, 773, 1080
AgsI TTSAA 5 cut(s) 125, 595, 907, 1202, 1394
AjnI CCWGG 1 cut(s) 443
AloI GAACNNNNNNTCC 4 cut(s) 271, 303, 1238, 1270
AluBI AGCT 7 cut(s) 43, 143, 968, 1029, 1034, 1387, 1437
AluI AGCT 7 cut(s) 43, 143, 968, 1029, 1034, 1387, 1437
Alw21I GWGCWC 4 cut(s) 145, 1273, 1389, 1515
Alw26I GTCTC 2 cut(s) 729, 1286
Alw44I GTGCAC 2 cut(s) 1269, 1511
AlwI GGATC 4 cut(s) 317, 839, 1186, 1677
AlwNI CAGNNNCTG 2 cut(s) 17, 232
Ama87I CYCGRG 3 cut(s) 1221, 1238, 1719
AoxI GGCC 2 cut(s) 677, 1323
ApaLI GTGCAC 2 cut(s) 1269, 1511
ApeKI GCWGC 3 cut(s) 223, 1340, 1739
ApoI RAATTY 6 cut(s) 439, 449, 738, 985, 1231, 1687
AseI ATTAAT 3 cut(s) 156, 162, 183
Asp700I GAANNNNTTC 1 cut(s) 1355
AspS9I GGNCC 3 cut(s) 708, 1323, 1500
AsuC2I CCSGG 1 cut(s) 530
AsuHPI GGTGA 2 cut(s) 103, 445
AvaI CYCGRG 3 cut(s) 1221, 1238, 1719
AvaII GGWCC 2 cut(s) 708, 1500
BaeGI GKGCMC 2 cut(s) 1273, 1515
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 3 cut(s) 145, 982, 1389
Bbv12I GWGCWC 4 cut(s) 145, 1273, 1389, 1515
BbvI GCAGC 2 cut(s) 210, 1352
BccI CCATC 3 cut(s) 109, 430, 434
BceAI ACGGC 4 cut(s) 286, 829, 1333, 1691
BciT130I CCWGG 1 cut(s) 445
BciVI GTATCC 1 cut(s) 1553
BclI TGATCA 2 cut(s) 867, 1005
BcnI CCSGG 1 cut(s) 530
BcoDI GTCTC 2 cut(s) 729, 1286
BfaI CTAG 4 cut(s) 509, 878, 965, 1506
BfmI CTRYAG 2 cut(s) 224, 1462
BfuI GTATCC 1 cut(s) 1553
BisI GCNGC 9 cut(s) 9, 224, 299, 498, 621, 1341, 1494, 1530, 1740
BlpI GCTNAGC 1 cut(s) 1030
BlsI GCNGC 9 cut(s) 10, 225, 300, 499, 622, 1342, 1495, 1531, 1741
Bme1390I CCNGG 2 cut(s) 445, 530
Bme18I GGWCC 2 cut(s) 708, 1500
BmeT110I CYCGRG 3 cut(s) 1221, 1238, 1719
BmgT120I GGNCC 3 cut(s) 708, 1323, 1500
BmiI GGNNCC 2 cut(s) 7, 979
BmrFI CCNGG 2 cut(s) 445, 530
BmsI GCATC 3 cut(s) 632, 915, 1308
Bpu1102I GCTNAGC 1 cut(s) 1030
BpuEI CTTGAG 3 cut(s) 65, 1045, 1168
BpuMI CCSGG 1 cut(s) 530
BsaBI GATNNNNATC 2 cut(s) 866, 906
BsaHI GRCGYC 3 cut(s) 777, 1367, 1458
BsaJI CCNNGG 2 cut(s) 529, 1581
BsaWI WCCGGW 1 cut(s) 1691
BsaXI ACNNNNNCTCC 6 cut(s) 271, 301, 325, 331, 355, 361
Bsc4I CCNNNNNNNGG 3 cut(s) 504, 773, 1080
Bse118I RCCGGY 1 cut(s) 1731
Bse1I ACTGG 1 cut(s) 884
Bse3DI GCAATG 1 cut(s) 1273
Bse8I GATNNNNATC 2 cut(s) 866, 906
BseBI CCWGG 1 cut(s) 445
BseDI CCNNGG 2 cut(s) 529, 1581
BseGI GGATG 3 cut(s) 422, 930, 954
BseJI GATNNNNATC 2 cut(s) 866, 906
BseLI CCNNNNNNNGG 3 cut(s) 504, 773, 1080
BseMI GCAATG 1 cut(s) 1273
BseMII CTCAG 7 cut(s) 158, 371, 407, 1117, 1139, 1199, 1297
BseNI ACTGG 1 cut(s) 884
BseRI GAGGAG 5 cut(s) 38, 53, 62, 339, 355
BseSI GKGCMC 2 cut(s) 1273, 1515
BseXI GCAGC 2 cut(s) 210, 1352
BseYI CCCAGC 2 cut(s) 384, 1320
BsgI GTGCAG 1 cut(s) 1352
Bsh1285I CGRYCG 1 cut(s) 1696
BshFI GGCC 2 cut(s) 679, 1325
BshNI GGYRCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 1696
BsiHKAI GWGCWC 4 cut(s) 145, 1273, 1389, 1515
BsiHKCI CYCGRG 3 cut(s) 1221, 1238, 1719
BsiSI CCGG 4 cut(s) 529, 735, 1692, 1732
BslFI GGGAC 3 cut(s) 366, 937, 1709
BslI CCNNNNNNNGG 3 cut(s) 504, 773, 1080
BsmAI GTCTC 2 cut(s) 729, 1286
BsmFI GGGAC 3 cut(s) 366, 937, 1709
BsmI GAATGC 2 cut(s) 388, 1116
BsnI GGCC 2 cut(s) 679, 1325
BsoBI CYCGRG 3 cut(s) 1221, 1238, 1719
Bsp1286I GDGCHC 5 cut(s) 145, 982, 1273, 1389, 1515
Bsp143I GATC 6 cut(s) 309, 831, 867, 1005, 1178, 1669
Bsp1720I GCTNAGC 1 cut(s) 1030
Bsp19I CCATGG 1 cut(s) 1581
BspANI GGCC 2 cut(s) 679, 1325
BspCNI CTCAG 7 cut(s) 157, 370, 406, 1116, 1138, 1200, 1296
BspLI GGNNCC 2 cut(s) 7, 979
BspMAI CTGCAG 2 cut(s) 228, 1466
BspPI GGATC 4 cut(s) 317, 839, 1186, 1677
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 1 cut(s) 1273
BsrFI RCCGGY 1 cut(s) 1731
BsrI ACTGG 1 cut(s) 884
BssAI RCCGGY 1 cut(s) 1731
BssECI CCNNGG 2 cut(s) 529, 1581
BssMI GATC 6 cut(s) 309, 831, 867, 1005, 1178, 1669
BssNI GRCGYC 3 cut(s) 777, 1367, 1458
BssT1I CCWWGG 1 cut(s) 1581
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 3 cut(s) 380, 493, 1075
BstACI GRCGYC 3 cut(s) 777, 1367, 1458
BstDEI CTNAG 8 cut(s) 144, 357, 393, 1030, 1103, 1125, 1208, 1283
BstDSI CCRYGG 1 cut(s) 1581
BstF5I GGATG 3 cut(s) 422, 930, 954
BstKTI GATC 6 cut(s) 312, 834, 870, 1008, 1181, 1672
BstMAI GTCTC 2 cut(s) 729, 1286
BstMBI GATC 6 cut(s) 309, 831, 867, 1005, 1178, 1669
BstMCI CGRYCG 1 cut(s) 1696
BstMWI GCNNNNNNNGC 7 cut(s) 140, 229, 298, 394, 1202, 1443, 1502
BstNI CCWGG 1 cut(s) 445
BstNSI RCATGY 1 cut(s) 1598
BstSCI CCNGG 2 cut(s) 443, 528
BstSFI CTRYAG 2 cut(s) 224, 1462
BstSLI GKGCMC 2 cut(s) 1273, 1515
BstV1I GCAGC 2 cut(s) 210, 1352
BstX2I RGATCY 2 cut(s) 309, 1178
BstYI RGATCY 2 cut(s) 309, 1178
BsuI GTATCC 1 cut(s) 1553
BsuRI GGCC 2 cut(s) 679, 1325
BtgI CCRYGG 1 cut(s) 1581
BtsCI GGATG 3 cut(s) 422, 930, 954
BtsI GCAGTG 2 cut(s) 672, 1471
BtsIMutI CAGTG 3 cut(s) 434, 672, 1471
CaiI CAGNNNCTG 2 cut(s) 17, 232
Cfr10I RCCGGY 1 cut(s) 1731
Cfr13I GGNCC 3 cut(s) 708, 1323, 1500
CseI GACGC 3 cut(s) 766, 1356, 1447
CviAII CATG 8 cut(s) 167, 212, 654, 721, 1258, 1291, 1582, 1595
DdeI CTNAG 8 cut(s) 144, 357, 393, 1030, 1103, 1125, 1208, 1283
DpnI GATC 6 cut(s) 311, 833, 869, 1007, 1180, 1671
DpnII GATC 6 cut(s) 309, 831, 867, 1005, 1178, 1669
DraI TTTAAA 1 cut(s) 1230
EaeI YGGCCR 1 cut(s) 677
EciI GGCGGA 2 cut(s) 95, 279
Ecl136II GAGCTC 2 cut(s) 143, 1387
Eco130I CCWWGG 1 cut(s) 1581
Eco24I GRGCYC 3 cut(s) 145, 982, 1389
Eco47I GGWCC 2 cut(s) 708, 1500
Eco53kI GAGCTC 2 cut(s) 143, 1387
Eco57I CTGAAG 1 cut(s) 1065
Eco88I CYCGRG 3 cut(s) 1221, 1238, 1719
EcoICRI GAGCTC 2 cut(s) 143, 1387
EcoRI GAATTC 3 cut(s) 439, 738, 1687
EcoRII CCWGG 1 cut(s) 443
EcoT14I CCWWGG 1 cut(s) 1581
EcoT38I GRGCYC 3 cut(s) 145, 982, 1389
ErhI CCWWGG 1 cut(s) 1581
FaeI CATG 8 cut(s) 170, 215, 657, 724, 1261, 1294, 1585, 1598
FaqI GGGAC 3 cut(s) 366, 937, 1709
FatI CATG 8 cut(s) 166, 211, 653, 720, 1257, 1290, 1581, 1594
FauI CCCGC 1 cut(s) 1736
FbaI TGATCA 2 cut(s) 867, 1005
Fnu4HI GCNGC 9 cut(s) 9, 224, 299, 498, 621, 1341, 1494, 1530, 1740
FokI GGATG 3 cut(s) 409, 937, 961
FriOI GRGCYC 3 cut(s) 145, 982, 1389
Fsp4HI GCNGC 9 cut(s) 9, 224, 299, 498, 621, 1341, 1494, 1530, 1740
FspBI CTAG 4 cut(s) 509, 878, 965, 1506
GluI GCNGC 9 cut(s) 9, 224, 299, 498, 621, 1341, 1494, 1530, 1740
GsaI CCCAGC 2 cut(s) 388, 1324
HaeIII GGCC 2 cut(s) 679, 1325
HapII CCGG 4 cut(s) 529, 735, 1692, 1732
HgaI GACGC 3 cut(s) 766, 1356, 1447
Hin1I GRCGYC 3 cut(s) 777, 1367, 1458
Hin1II CATG 8 cut(s) 170, 215, 657, 724, 1261, 1294, 1585, 1598
HinfI GANTC 9 cut(s) 235, 551, 783, 907, 961, 1287, 1421, 1633, 1722
HpaII CCGG 4 cut(s) 529, 735, 1692, 1732
HphI GGTGA 2 cut(s) 103, 445
Hpy166II GTNNAC 3 cut(s) 694, 1271, 1513
Hpy188III TCNNGA 4 cut(s) 1024, 1360, 1637, 1697
Hpy8I GTNNAC 3 cut(s) 694, 1271, 1513
Hpy99I CGWCG 2 cut(s) 782, 1372
HpyAV CCTTC 5 cut(s) 194, 363, 808, 1336, 1345
HpyCH4III ACNGT 3 cut(s) 380, 493, 1075
HpyCH4IV ACGT 1 cut(s) 1059
HpyF10VI GCNNNNNNNGC 7 cut(s) 140, 229, 298, 394, 1202, 1443, 1502
HpyF3I CTNAG 8 cut(s) 144, 357, 393, 1030, 1103, 1125, 1208, 1283
HpySE526I ACGT 1 cut(s) 1059
Hsp92I GRCGYC 3 cut(s) 777, 1367, 1458
Hsp92II CATG 8 cut(s) 170, 215, 657, 724, 1261, 1294, 1585, 1598
Ksp22I TGATCA 2 cut(s) 867, 1005
Kzo9I GATC 6 cut(s) 309, 831, 867, 1005, 1178, 1669
LmnI GCTCC 5 cut(s) 40, 82, 140, 977, 1384
Lsp1109I GCAGC 2 cut(s) 210, 1352
LweI GCATC 3 cut(s) 632, 915, 1308
MaeI CTAG 4 cut(s) 509, 878, 965, 1506
MaeII ACGT 1 cut(s) 1059
MaeIII GTNAC 2 cut(s) 303, 1610
MalI GATC 6 cut(s) 311, 833, 869, 1007, 1180, 1671
MboI GATC 6 cut(s) 309, 831, 867, 1005, 1178, 1669
MboII GAAGA 5 cut(s) 137, 512, 1259, 1294, 1543
MfeI CAATTG 1 cut(s) 1197
MflI RGATCY 2 cut(s) 309, 1178
MhlI GDGCHC 5 cut(s) 145, 982, 1273, 1389, 1515
MlyI GAGTC 3 cut(s) 792, 1296, 1731
MmeI TCCRAC 5 cut(s) 263, 760, 809, 916, 1449
MroXI GAANNNNTTC 1 cut(s) 1355
MseI TTAA 7 cut(s) 156, 162, 183, 537, 570, 632, 1229
MslI CAYNNNNRTG 3 cut(s) 652, 1487, 1599
MspI CCGG 4 cut(s) 529, 735, 1692, 1732
MspR9I CCNGG 2 cut(s) 445, 530
MunI CAATTG 1 cut(s) 1197
Mva1269I GAATGC 2 cut(s) 388, 1116
MvaI CCWGG 1 cut(s) 445
MwoI GCNNNNNNNGC 7 cut(s) 140, 229, 298, 394, 1202, 1443, 1502
NciI CCSGG 1 cut(s) 530
NcoI CCATGG 1 cut(s) 1581
NdeII GATC 6 cut(s) 309, 831, 867, 1005, 1178, 1669
NlaIII CATG 8 cut(s) 170, 215, 657, 724, 1261, 1294, 1585, 1598
NlaIV GGNNCC 2 cut(s) 7, 979
NmeAIII GCCGAG 1 cut(s) 604
NmuCI GTSAC 1 cut(s) 303
NspI RCATGY 1 cut(s) 1598
PaeR7I CTCGAG 2 cut(s) 1238, 1719
PctI GAATGC 2 cut(s) 388, 1116
PdmI GAANNNNTTC 1 cut(s) 1355
PfeI GAWTC 6 cut(s) 235, 551, 907, 961, 1421, 1633
PfoI TCCNGGA 1 cut(s) 443
PkrI GCNGC 9 cut(s) 10, 225, 300, 499, 622, 1342, 1495, 1531, 1741
PleI GAGTC 3 cut(s) 791, 1295, 1730
PpsI GAGTC 3 cut(s) 791, 1295, 1730
PshBI ATTAAT 3 cut(s) 156, 162, 183
Psp124BI GAGCTC 2 cut(s) 145, 1389
Psp1406I AACGTT 1 cut(s) 1059
Psp6I CCWGG 1 cut(s) 443
PspFI CCCAGC 2 cut(s) 384, 1320
PspGI CCWGG 1 cut(s) 443
PspN4I GGNNCC 2 cut(s) 7, 979
PspPI GGNCC 3 cut(s) 708, 1323, 1500
PspXI VCTCGAGB 2 cut(s) 1238, 1719
PstI CTGCAG 2 cut(s) 228, 1466
PstNI CAGNNNCTG 2 cut(s) 17, 232
PsuI RGATCY 2 cut(s) 309, 1178
RseI CAYNNNNRTG 3 cut(s) 652, 1487, 1599
SacI GAGCTC 2 cut(s) 145, 1389
SaqAI TTAA 7 cut(s) 156, 162, 183, 537, 570, 632, 1229
SatI GCNGC 9 cut(s) 9, 224, 299, 498, 621, 1341, 1494, 1530, 1740
Sau3AI GATC 6 cut(s) 309, 831, 867, 1005, 1178, 1669
Sau96I GGNCC 3 cut(s) 708, 1323, 1500
SchI GAGTC 3 cut(s) 792, 1296, 1731
ScrFI CCNGG 2 cut(s) 445, 530
SduI GDGCHC 5 cut(s) 145, 982, 1273, 1389, 1515
SfaNI GCATC 3 cut(s) 632, 915, 1308
SfcI CTRYAG 2 cut(s) 224, 1462
Sfr274I CTCGAG 2 cut(s) 1238, 1719
SinI GGWCC 2 cut(s) 708, 1500
SlaI CTCGAG 2 cut(s) 1238, 1719
SmiMI CAYNNNNRTG 3 cut(s) 652, 1487, 1599
SmlI CTYRAG 5 cut(s) 44, 1024, 1147, 1238, 1719
SmoI CTYRAG 5 cut(s) 44, 1024, 1147, 1238, 1719
SspI AATATT 3 cut(s) 154, 160, 181
SspMI CTAG 4 cut(s) 509, 878, 965, 1506
SstI GAGCTC 2 cut(s) 145, 1389
StyD4I CCNGG 2 cut(s) 443, 528
StyI CCWWGG 1 cut(s) 1581
TaaI ACNGT 3 cut(s) 380, 493, 1075
TaiI ACGT 1 cut(s) 1062
TaqI TCGA 5 cut(s) 615, 1239, 1653, 1696, 1720
TauI GCSGC 6 cut(s) 11, 301, 500, 623, 1496, 1532
TfiI GAWTC 6 cut(s) 235, 551, 907, 961, 1421, 1633
Tru1I TTAA 7 cut(s) 156, 162, 183, 537, 570, 632, 1229
Tru9I TTAA 7 cut(s) 156, 162, 183, 537, 570, 632, 1229
TscAI CASTG 3 cut(s) 441, 672, 1471
TseFI GTSAC 1 cut(s) 303
TseI GCWGC 3 cut(s) 223, 1340, 1739
Tsp45I GTSAC 1 cut(s) 303
TspDTI ATGAA 5 cut(s) 200, 688, 1246, 1543, 1700
TspGWI ACGGA 1 cut(s) 322
TspRI CASTG 3 cut(s) 441, 672, 1471
VneI GTGCAC 2 cut(s) 1269, 1511
VpaK11BI GGWCC 2 cut(s) 708, 1500
VspI ATTAAT 3 cut(s) 156, 162, 183
XapI RAATTY 6 cut(s) 439, 449, 738, 985, 1231, 1687
XceI RCATGY 1 cut(s) 1598
XhoI CTCGAG 2 cut(s) 1238, 1719
XmnI GAANNNNTTC 1 cut(s) 1355
XspI CTAG 4 cut(s) 509, 878, 965, 1506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.