Rw1G002670

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002251
Physical Location & Seq
Reverse (-)
4796527 .. 4799675
3149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G002670.1

Sequence Viewer

Length: 1761 bp
ATGGGTGCCGCTGAACCCGAAGTGGTGGAGAGAGAAAGTGAAGAGGTTGAGGCGAGGGAGAGAGATGAGAGGGAGGAGGAGGAAGAGGAGGAGGAGAGGAGAGAGCTGGAAGTGAGAGATGTGGAGAAAGTTGAGGTGGGTTTTGAACAACAGAGCAATATTAATCATGATCAGAGTAGTCAGGGAGATGTTCATGTGTCAATGTTGCAGAGGCTTAATCTTACAAACCCTTTGAGAATTGCTATCAATACTAAAACCAGAAGCGCTGCTCCTCCTCCTCCTCCTCGTCGGTTCCAACAGGCCCAAAATGTCAGAGTTGGGACTACTCCATCTCCATCTCTATCTGATTTCTCTCAGTCTCACACAACTCCTTCTCACGCAACTCCTCCTCCTCCTCCTCAGCATTCTCAGCCTCGCTCTATTCCAACCCCTCAACCAGCGATCACAACACTGAACTCAAGAGCCTACACCAACAAGATATCTCTGTTCCTTTTCGTTGTGCACATGGTCGGCGCTATTGGGCTAGTTGGTTTTCTGACATTCAAGGGAGTTCAGGGTCTGATACAAGATTCAGGCAGTATTTCGAGAAAGGAGAAAAGGGTGCTAAAGTATTTTCTACCACAAGTGGAAGCTGCATCTCTTATGAGCATTACTCTTGCATTTGCATGGCAAAAGGCATTTAGGAAATGGCCTACATTTATGGTTCATTTCATACTATGGAACACTTTTGTCATGAGTTTATCTGCTGGAATCCTGCTAATTTGTTTCCAAAAGCCCACCACCACTGGCGTGGGGGTCTGTTTCATTGGCTTTGCCATAGGGAATAGCTTGTACGCTTGTTGGATCACGCCGCAGATAGGATTTTGTATCAGGATATTGATCAAGTCGCTCGAACCTGTATCAAAGTTCCGCGATTTGAATCGACCCACTTACTGGATGCTTGGGTTCGGATTTTTGTGGATGTCTCTATGGATTTTAGGAGTCATTGGAGCCTTGAATTTCTACTTTCCTCCACTAGTCATAATTGCATTGGTATTGAGCCTGATGTGGACAGCCGAAGTAATGAGGAATGTAGTTAATATAACTGTGAGTAGGGTGATTGCTCTATATTATCTCAGAGGAATGCAGTCCAATACTCAGTTTTGTTTCCAGAGAGCCTTGAGTAAAAATCTTGGAAGTGCTTGTTTTGGATCTCTGTTTGTGCCAGCAATTGAAGCGCTGAGGATTGTTGCTCGAGCTTTAAATCTACTTGAGGGAGAGGATGAGTTCATGTTTTCTTGTGCGCATTGCTGTCTCCATGTCATGCAGTGCATCTTCCGATATGGCAATGGCTGGGCCTTTGTTCAGATAGCAGCATATGGAAAAGATTTTGTGAGGGCATCACAAGAGACTTGGGAGCTCTTTGAAAGGCAAGAAATTCATCTCCTTGTTGATTCTGACATCACCAGCTCAATATGCTTCCTAACCGGCATCTGCAGTGGCTCTATGTGTGTCATAGTTGTGGCTGCTTGGACCGCCACAGTACGCCAAAGTTTCACGGCCACCATTTCCCTGCTTGCATTCTTCATTGGATACCTTATGACCAGGATCGCAATGGCACTACCACATGCCTGTGTAGGATGTTACTACGCCTGCTATGCAGAGAATCCAGACAACAGATTATTTGACAACACAATAAAGGATCGTCTTGCTCTGTTGAAATCTCGACCAGATGTACTCGTCCCCACACCTCGAGTCCCTCGCCGGTTCGCTGCAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

586

Amino Acids

65.96

Weight (kDa)

8.42

Isoelectric Point (pI)

49.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 267 - 551 3.2e-37 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1284
AccB1I GGYRCC 1 cut(s) 5
AccB7I CCANNNNNTGG 1 cut(s) 933
AccII CGCG 1 cut(s) 912
AciI CCGC 4 cut(s) 9, 851, 910, 1515
AclWI GGATC 4 cut(s) 851, 1198, 1595, 1689
AcoI YGGCCR 1 cut(s) 1539
AcsI RAATTY 2 cut(s) 997, 1416
AfaI GTAC 3 cut(s) 833, 1524, 1716
AfeI AGCGCT 2 cut(s) 265, 1218
AfiI CCNNNNNNNGG 2 cut(s) 857, 933
AgsI TTSAA 7 cut(s) 146, 544, 919, 997, 1214, 1406, 1699
AhlI ACTAGT 1 cut(s) 1015
AjnI CCWGG 1 cut(s) 1583
AleI CACNNNNGTG 1 cut(s) 788
AluBI AGCT 6 cut(s) 106, 632, 828, 1238, 1399, 1449
AluI AGCT 6 cut(s) 106, 632, 828, 1238, 1399, 1449
Alw21I GWGCWC 2 cut(s) 504, 1401
Alw26I GTCTC 4 cut(s) 363, 969, 1298, 1382
Alw44I GTGCAC 1 cut(s) 500
AlwI GGATC 4 cut(s) 851, 1198, 1595, 1689
AlwNI CAGNNNCTG 1 cut(s) 559
Ama87I CYCGRG 2 cut(s) 1233, 1731
Aor51HI AGCGCT 2 cut(s) 265, 1218
AoxI GGCC 4 cut(s) 300, 689, 1335, 1539
ApaLI GTGCAC 1 cut(s) 500
ApeKI GCWGC 5 cut(s) 266, 632, 1352, 1505, 1751
ApoI RAATTY 2 cut(s) 997, 1416
AseI ATTAAT 1 cut(s) 162
AspLEI GCGC 4 cut(s) 266, 515, 1219, 1285
AspS9I GGNCC 3 cut(s) 301, 1335, 1512
AsuHPI GGTGA 2 cut(s) 1108, 1435
AvaI CYCGRG 2 cut(s) 1233, 1731
AvaII GGWCC 1 cut(s) 1512
BaeGI GKGCMC 1 cut(s) 504
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 1401
Bbv12I GWGCWC 2 cut(s) 504, 1401
BbvCI CCTCAGC 2 cut(s) 399, 1220
BbvI GCAGC 5 cut(s) 253, 619, 1364, 1492, 1738
BccI CCATC 2 cut(s) 337, 343
BceAI ACGGC 1 cut(s) 1554
BciT130I CCWGG 1 cut(s) 1585
BciVI GTATCC 1 cut(s) 1565
BclI TGATCA 2 cut(s) 169, 879
BcoDI GTCTC 4 cut(s) 363, 969, 1298, 1382
BcuI ACTAGT 1 cut(s) 1015
BfaI CTAG 2 cut(s) 524, 1016
BfmI CTRYAG 1 cut(s) 1474
BfoI RGCGCY 3 cut(s) 267, 516, 1220
BfuI GTATCC 1 cut(s) 1565
BisI GCNGC 7 cut(s) 9, 267, 633, 851, 1353, 1506, 1752
BlsI GCNGC 7 cut(s) 10, 268, 634, 852, 1354, 1507, 1753
Bme1390I CCNGG 1 cut(s) 1585
Bme18I GGWCC 1 cut(s) 1512
BmeT110I CYCGRG 2 cut(s) 1233, 1731
BmgT120I GGNCC 3 cut(s) 301, 1335, 1512
BmiI GGNNCC 3 cut(s) 7, 293, 991
BmrFI CCNGG 1 cut(s) 1585
BmsI GCATC 5 cut(s) 644, 927, 1320, 1388, 1479
BplI GAGNNNNNCTC 2 cut(s) 637, 669
Bpu10I CCTNAGC 2 cut(s) 399, 1220
BpuEI CTTGAG 3 cut(s) 442, 1180, 1271
BsaBI GATNNNNATC 2 cut(s) 878, 918
BsaXI ACNNNNNCTCC 4 cut(s) 316, 346, 373, 403
Bsc4I CCNNNNNNNGG 2 cut(s) 857, 933
Bse118I RCCGGY 2 cut(s) 1466, 1743
Bse1I ACTGG 2 cut(s) 790, 938
Bse3DI GCAATG 3 cut(s) 1285, 1333, 1599
Bse8I GATNNNNATC 2 cut(s) 878, 918
BseBI CCWGG 1 cut(s) 1585
BseGI GGATG 4 cut(s) 942, 966, 1267, 1625
BseJI GATNNNNATC 2 cut(s) 878, 918
BseLI CCNNNNNNNGG 2 cut(s) 857, 933
BseMI GCAATG 3 cut(s) 1285, 1333, 1599
BseMII CTCAG 6 cut(s) 368, 413, 422, 1129, 1151, 1211
BseNI ACTGG 2 cut(s) 790, 938
BseSI GKGCMC 1 cut(s) 504
BseXI GCAGC 5 cut(s) 253, 619, 1364, 1492, 1738
BseYI CCCAGC 1 cut(s) 1332
Bsh1236I CGCG 1 cut(s) 912
BshFI GGCC 4 cut(s) 302, 691, 1337, 1541
BshNI GGYRCC 1 cut(s) 5
BsiHKAI GWGCWC 2 cut(s) 504, 1401
BsiHKCI CYCGRG 2 cut(s) 1233, 1731
BsiSI CCGG 2 cut(s) 1467, 1744
BslFI GGGAC 3 cut(s) 334, 1706, 1721
BslI CCNNNNNNNGG 2 cut(s) 857, 933
BsmAI GTCTC 4 cut(s) 363, 969, 1298, 1382
BsmFI GGGAC 3 cut(s) 334, 1706, 1721
BsmI GAATGC 3 cut(s) 403, 1128, 1559
BsnI GGCC 4 cut(s) 302, 691, 1337, 1541
BsoBI CYCGRG 2 cut(s) 1233, 1731
Bsp1286I GDGCHC 2 cut(s) 504, 1401
Bsp143I GATC 7 cut(s) 169, 441, 843, 879, 1190, 1587, 1681
BspACI CCGC 4 cut(s) 9, 851, 910, 1515
BspANI GGCC 4 cut(s) 302, 691, 1337, 1541
BspCNI CTCAG 6 cut(s) 367, 412, 421, 1128, 1150, 1212
BspFNI CGCG 1 cut(s) 912
BspHI TCATGA 2 cut(s) 166, 732
BspLI GGNNCC 3 cut(s) 7, 293, 991
BspMAI CTGCAG 1 cut(s) 1478
BspPI GGATC 4 cut(s) 851, 1198, 1595, 1689
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 3 cut(s) 1285, 1333, 1599
BsrFI RCCGGY 2 cut(s) 1466, 1743
BsrI ACTGG 2 cut(s) 790, 938
BssAI RCCGGY 2 cut(s) 1466, 1743
BssMI GATC 7 cut(s) 169, 441, 843, 879, 1190, 1587, 1681
Bst2UI CCWGG 1 cut(s) 1585
Bst4CI ACNGT 2 cut(s) 1087, 1522
Bst6I CTCTTC 2 cut(s) 36, 78
BstC8I GCNNGC 3 cut(s) 1206, 1557, 1633
BstDEI CTNAG 6 cut(s) 354, 399, 408, 1115, 1137, 1220
BstF5I GGATG 4 cut(s) 942, 966, 1267, 1625
BstFNI CGCG 1 cut(s) 912
BstH2I RGCGCY 3 cut(s) 267, 516, 1220
BstHHI GCGC 4 cut(s) 266, 515, 1219, 1285
BstKTI GATC 7 cut(s) 172, 444, 846, 882, 1193, 1590, 1684
BstMAI GTCTC 4 cut(s) 363, 969, 1298, 1382
BstMBI GATC 7 cut(s) 169, 441, 843, 879, 1190, 1587, 1681
BstMWI GCNNNNNNNGC 5 cut(s) 409, 1214, 1455, 1514, 1637
BstNI CCWGG 1 cut(s) 1585
BstNSI RCATGY 1 cut(s) 1610
BstSCI CCNGG 1 cut(s) 1583
BstSFI CTRYAG 1 cut(s) 1474
BstSLI GKGCMC 1 cut(s) 504
BstUI CGCG 1 cut(s) 912
BstV1I GCAGC 5 cut(s) 253, 619, 1364, 1492, 1738
BstX2I RGATCY 1 cut(s) 1190
BstXI CCANNNNNNTGG 1 cut(s) 790
BstYI RGATCY 1 cut(s) 1190
BsuI GTATCC 1 cut(s) 1565
BsuRI GGCC 4 cut(s) 302, 691, 1337, 1541
BtsCI GGATG 4 cut(s) 942, 966, 1267, 1625
BtsI GCAGTG 2 cut(s) 1313, 1483
BtsIMutI CAGTG 4 cut(s) 449, 783, 1313, 1483
Cac8I GCNNGC 3 cut(s) 1206, 1557, 1633
CaiI CAGNNNCTG 1 cut(s) 559
CciI TCATGA 2 cut(s) 166, 732
CfoI GCGC 4 cut(s) 266, 515, 1219, 1285
Cfr10I RCCGGY 2 cut(s) 1466, 1743
Cfr13I GGNCC 3 cut(s) 301, 1335, 1512
Csp6I GTAC 3 cut(s) 832, 1523, 1715
CviAII CATG 9 cut(s) 167, 194, 505, 666, 733, 1270, 1298, 1303, 1607
CviQI GTAC 3 cut(s) 832, 1523, 1715
DdeI CTNAG 6 cut(s) 354, 399, 408, 1115, 1137, 1220
DpnI GATC 7 cut(s) 171, 443, 845, 881, 1192, 1589, 1683
DpnII GATC 7 cut(s) 169, 441, 843, 879, 1190, 1587, 1681
DraI TTTAAA 1 cut(s) 1242
EaeI YGGCCR 1 cut(s) 1539
Eam1104I CTCTTC 2 cut(s) 36, 78
EarI CTCTTC 2 cut(s) 36, 78
Ecl136II GAGCTC 1 cut(s) 1399
Eco24I GRGCYC 1 cut(s) 1401
Eco32I GATATC 1 cut(s) 480
Eco47I GGWCC 1 cut(s) 1512
Eco47III AGCGCT 2 cut(s) 265, 1218
Eco53kI GAGCTC 1 cut(s) 1399
Eco88I CYCGRG 2 cut(s) 1233, 1731
EcoICRI GAGCTC 1 cut(s) 1399
EcoRII CCWGG 1 cut(s) 1583
EcoRV GATATC 1 cut(s) 480
EcoT38I GRGCYC 1 cut(s) 1401
FaeI CATG 9 cut(s) 170, 197, 508, 669, 736, 1273, 1301, 1306, 1610
FaqI GGGAC 3 cut(s) 334, 1706, 1721
FatI CATG 9 cut(s) 166, 193, 504, 665, 732, 1269, 1297, 1302, 1606
FauNDI CATATG 1 cut(s) 1357
FbaI TGATCA 2 cut(s) 169, 879
Fnu4HI GCNGC 7 cut(s) 9, 267, 633, 851, 1353, 1506, 1752
FokI GGATG 4 cut(s) 949, 973, 1274, 1632
FriOI GRGCYC 1 cut(s) 1401
Fsp4HI GCNGC 7 cut(s) 9, 267, 633, 851, 1353, 1506, 1752
FspAI RTGCGCAY 1 cut(s) 1284
FspBI CTAG 2 cut(s) 524, 1016
FspI TGCGCA 1 cut(s) 1284
GlaI GCGC 4 cut(s) 265, 514, 1218, 1284
GluI GCNGC 7 cut(s) 9, 267, 633, 851, 1353, 1506, 1752
GsaI CCCAGC 1 cut(s) 1336
HaeII RGCGCY 3 cut(s) 267, 516, 1220
HaeIII GGCC 4 cut(s) 302, 691, 1337, 1541
HapII CCGG 2 cut(s) 1467, 1744
HhaI GCGC 4 cut(s) 266, 515, 1219, 1285
Hin1II CATG 9 cut(s) 170, 197, 508, 669, 736, 1273, 1301, 1306, 1610
Hin6I GCGC 4 cut(s) 264, 513, 1217, 1283
HinP1I GCGC 4 cut(s) 264, 513, 1217, 1283
HinfI GANTC 7 cut(s) 569, 750, 919, 981, 1433, 1645, 1734
HpaII CCGG 2 cut(s) 1467, 1744
HphI GGTGA 2 cut(s) 1108, 1435
Hpy166II GTNNAC 2 cut(s) 502, 1050
Hpy188III TCNNGA 8 cut(s) 167, 459, 585, 733, 871, 1150, 1649, 1704
Hpy8I GTNNAC 2 cut(s) 502, 1050
Hpy99I CGWCG 1 cut(s) 291
HpyAV CCTTC 1 cut(s) 381
HpyCH4III ACNGT 2 cut(s) 1087, 1522
HpyF10VI GCNNNNNNNGC 5 cut(s) 409, 1214, 1455, 1514, 1637
HpyF3I CTNAG 6 cut(s) 354, 399, 408, 1115, 1137, 1220
Hsp92II CATG 9 cut(s) 170, 197, 508, 669, 736, 1273, 1301, 1306, 1610
HspAI GCGC 4 cut(s) 264, 513, 1217, 1283
Ksp22I TGATCA 2 cut(s) 169, 879
Kzo9I GATC 7 cut(s) 169, 441, 843, 879, 1190, 1587, 1681
LmnI GCTCC 3 cut(s) 274, 989, 1396
Lsp1109I GCAGC 5 cut(s) 253, 619, 1364, 1492, 1738
LweI GCATC 5 cut(s) 644, 927, 1320, 1388, 1479
MaeI CTAG 2 cut(s) 524, 1016
MaeIII GTNAC 1 cut(s) 1622
MalI GATC 7 cut(s) 171, 443, 845, 881, 1192, 1589, 1683
MboI GATC 7 cut(s) 169, 441, 843, 879, 1190, 1587, 1681
MboII GAAGA 4 cut(s) 53, 95, 1306, 1555
MfeI CAATTG 1 cut(s) 1209
MflI RGATCY 1 cut(s) 1190
MhlI GDGCHC 2 cut(s) 504, 1401
MluCI AATT 6 cut(s) 237, 759, 997, 1023, 1209, 1416
MlyI GAGTC 2 cut(s) 990, 1743
MmeI TCCRAC 3 cut(s) 319, 449, 821
MseI TTAA 4 cut(s) 162, 216, 1077, 1241
MslI CAYNNNNRTG 4 cut(s) 664, 788, 1499, 1611
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 2 cut(s) 1467, 1744
MspR9I CCNGG 1 cut(s) 1585
MunI CAATTG 1 cut(s) 1209
Mva1269I GAATGC 3 cut(s) 403, 1128, 1559
MvaI CCWGG 1 cut(s) 1585
MvnI CGCG 1 cut(s) 912
MwoI GCNNNNNNNGC 5 cut(s) 409, 1214, 1455, 1514, 1637
NdeI CATATG 1 cut(s) 1357
NdeII GATC 7 cut(s) 169, 441, 843, 879, 1190, 1587, 1681
NlaIII CATG 9 cut(s) 170, 197, 508, 669, 736, 1273, 1301, 1306, 1610
NlaIV GGNNCC 3 cut(s) 7, 293, 991
NsbI TGCGCA 1 cut(s) 1284
NspI RCATGY 1 cut(s) 1610
OliI CACNNNNGTG 1 cut(s) 788
PaeR7I CTCGAG 2 cut(s) 1233, 1731
PagI TCATGA 2 cut(s) 166, 732
PctI GAATGC 3 cut(s) 403, 1128, 1559
PfeI GAWTC 5 cut(s) 569, 750, 919, 1433, 1645
PflMI CCANNNNNTGG 1 cut(s) 933
PkrI GCNGC 7 cut(s) 10, 268, 634, 852, 1354, 1507, 1753
PleI GAGTC 2 cut(s) 989, 1742
PpsI GAGTC 2 cut(s) 989, 1742
PshBI ATTAAT 1 cut(s) 162
Psp124BI GAGCTC 1 cut(s) 1401
Psp6I CCWGG 1 cut(s) 1583
PspFI CCCAGC 1 cut(s) 1332
PspGI CCWGG 1 cut(s) 1583
PspN4I GGNNCC 3 cut(s) 7, 293, 991
PspPI GGNCC 3 cut(s) 301, 1335, 1512
PspXI VCTCGAGB 2 cut(s) 1233, 1731
PstI CTGCAG 1 cut(s) 1478
PstNI CAGNNNCTG 1 cut(s) 559
PsuI RGATCY 1 cut(s) 1190
RsaI GTAC 3 cut(s) 833, 1524, 1716
RsaNI GTAC 3 cut(s) 832, 1523, 1715
RseI CAYNNNNRTG 4 cut(s) 664, 788, 1499, 1611
SacI GAGCTC 1 cut(s) 1401
SaqAI TTAA 4 cut(s) 162, 216, 1077, 1241
SatI GCNGC 7 cut(s) 9, 267, 633, 851, 1353, 1506, 1752
Sau3AI GATC 7 cut(s) 169, 441, 843, 879, 1190, 1587, 1681
Sau96I GGNCC 3 cut(s) 301, 1335, 1512
SchI GAGTC 2 cut(s) 990, 1743
ScrFI CCNGG 1 cut(s) 1585
SduI GDGCHC 2 cut(s) 504, 1401
SfaNI GCATC 5 cut(s) 644, 927, 1320, 1388, 1479
SfcI CTRYAG 1 cut(s) 1474
Sfr274I CTCGAG 2 cut(s) 1233, 1731
SinI GGWCC 1 cut(s) 1512
SlaI CTCGAG 2 cut(s) 1233, 1731
SmiMI CAYNNNNRTG 4 cut(s) 664, 788, 1499, 1611
SmlI CTYRAG 5 cut(s) 457, 1159, 1233, 1250, 1731
SmoI CTYRAG 5 cut(s) 457, 1159, 1233, 1250, 1731
SpeI ACTAGT 1 cut(s) 1015
Sse9I AATT 6 cut(s) 237, 759, 997, 1023, 1209, 1416
SsiI CCGC 4 cut(s) 9, 851, 910, 1515
SspI AATATT 1 cut(s) 160
SspMI CTAG 2 cut(s) 524, 1016
SstI GAGCTC 1 cut(s) 1401
StyD4I CCNGG 1 cut(s) 1583
TaaI ACNGT 2 cut(s) 1087, 1522
TaqI TCGA 6 cut(s) 584, 891, 922, 1234, 1705, 1732
TasI AATT 6 cut(s) 237, 759, 997, 1023, 1209, 1416
TatI WGTACW 1 cut(s) 1714
TauI GCSGC 2 cut(s) 11, 853
TfiI GAWTC 5 cut(s) 569, 750, 919, 1433, 1645
Tru1I TTAA 4 cut(s) 162, 216, 1077, 1241
Tru9I TTAA 4 cut(s) 162, 216, 1077, 1241
TscAI CASTG 4 cut(s) 456, 790, 1313, 1483
TseI GCWGC 5 cut(s) 266, 632, 1352, 1505, 1751
TspDTI ATGAA 7 cut(s) 182, 695, 700, 793, 1258, 1409, 1555
TspRI CASTG 4 cut(s) 456, 790, 1313, 1483
Van91I CCANNNNNTGG 1 cut(s) 933
VneI GTGCAC 1 cut(s) 500
VpaK11BI GGWCC 1 cut(s) 1512
VspI ATTAAT 1 cut(s) 162
XapI RAATTY 2 cut(s) 997, 1416
XceI RCATGY 1 cut(s) 1610
XcmI CCANNNNNNNNNTGG 2 cut(s) 787, 1591
XhoI CTCGAG 2 cut(s) 1233, 1731
XspI CTAG 2 cut(s) 524, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.