Rh2DG212500

D-aminoacyl-tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002251
Physical Location & Seq
Forward (+)
20226762 .. 20235854
9093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG212500.1

Sequence Viewer

Length: 642 bp
ATGTGCGAGGTCTTCTGCGACGAGCTCCCTAAAACCACCAAGGCAGTACAGATGATTGCTGGAAGAGGCAGGATGCAGCTGAAGAAAGATGATGTCTGGGTGGGCCGTCTAATTTCGAGTTATTTGTTACCAATGGAAGATCCGGGCCAGTCCAAGACAGAAACAAATACAAAAGATATCGGTGGAACTTGGAAACAATCAATCAAAGTAGCATTTGAGGCTACGCAATCGGCTTTCCCTGTCTCACACAACTCCTCCTCAGCATTCTCAGCCTCGCACTATTCCAACCCCTCAGGCAGCAATTACAACACTGAACTCAAGAGCCTACACCAACAAGATATCTTTGTTCCTTTTCATTGTGCACATGCAATTGAAGCACTGAGAATTGTTGCTCAGGCTTTAAATCTACTTGAGGGCGAGGATGGGTTTATGTTTTCTTCTGCGCATTGCTTTCTCAATGTCATGCAGTGCATCTTTCGATATGGCGATGGCTGGGCCTTTGTTCAGATATATATTCCAACAAACCCTTCTGGTGCTGAAACACTACCACCAGGAATTGTTGTTCCACAAACTGATTTGTATATGCACAGATTATGGGGTAAACCCAGTGAGGATCTAAAAACCAGCCAAAGTACTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_deacylase

Protein Analysis

213

Amino Acids

23.73

Weight (kDa)

5.95

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 174 - 210 7e-06 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 444
AclWI GGATC 2 cut(s) 134, 621
AcuI CTGAAG 1 cut(s) 101
AfaI GTAC 2 cut(s) 48, 634
AgsI TTSAA 1 cut(s) 374
AjnI CCWGG 1 cut(s) 550
AjuI GAANNNNNNNTTGG 2 cut(s) 511, 543
AloI GAACNNNNNNTCC 2 cut(s) 546, 578
AluBI AGCT 2 cut(s) 25, 79
AluI AGCT 2 cut(s) 25, 79
Alw21I GWGCWC 2 cut(s) 27, 364
Alw26I GTCTC 1 cut(s) 247
Alw44I GTGCAC 1 cut(s) 360
AlwI GGATC 2 cut(s) 134, 621
AoxI GGCC 3 cut(s) 103, 145, 495
ApaLI GTGCAC 1 cut(s) 360
ApeKI GCWGC 2 cut(s) 76, 297
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 3 cut(s) 103, 145, 495
AsuC2I CCSGG 1 cut(s) 144
AxyI CCTNAGG 1 cut(s) 292
BaeGI GKGCMC 1 cut(s) 364
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 4
Bbv12I GWGCWC 2 cut(s) 27, 364
BbvCI CCTCAGC 1 cut(s) 259
BbvI GCAGC 2 cut(s) 88, 309
BccI CCATC 2 cut(s) 416, 482
BceAI ACGGC 1 cut(s) 90
BciT130I CCWGG 1 cut(s) 552
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 247
BisI GCNGC 2 cut(s) 77, 298
BlsI GCNGC 2 cut(s) 78, 299
BmcAI AGTACT 1 cut(s) 634
Bme1390I CCNGG 2 cut(s) 144, 552
BmgT120I GGNCC 3 cut(s) 103, 145, 495
BmrFI CCNGG 2 cut(s) 144, 552
BmrI ACTGGG 1 cut(s) 600
BmsI GCATC 2 cut(s) 63, 480
BmuI ACTGGG 1 cut(s) 600
BpiI GAAGAC 1 cut(s) 4
Bpu10I CCTNAGC 2 cut(s) 259, 393
BpuEI CTTGAG 2 cut(s) 302, 431
BpuMI CCSGG 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 39
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bse1I ACTGG 2 cut(s) 148, 606
Bse21I CCTNAGG 1 cut(s) 292
Bse3DI GCAATG 1 cut(s) 445
BseBI CCWGG 1 cut(s) 552
BseDI CCNNGG 1 cut(s) 39
BseGI GGATG 2 cut(s) 78, 427
BseMI GCAATG 1 cut(s) 445
BseMII CTCAG 5 cut(s) 273, 282, 306, 371, 407
BseNI ACTGG 2 cut(s) 148, 606
BseRI GAGGAG 2 cut(s) 244, 247
BseSI GKGCMC 1 cut(s) 364
BseXI GCAGC 2 cut(s) 88, 309
BseYI CCCAGC 1 cut(s) 492
BshFI GGCC 3 cut(s) 105, 147, 497
BsiHKAI GWGCWC 2 cut(s) 27, 364
BsiSI CCGG 1 cut(s) 143
BsmAI GTCTC 1 cut(s) 247
BsmI GAATGC 1 cut(s) 263
BsnI GGCC 3 cut(s) 105, 147, 497
Bsp1286I GDGCHC 2 cut(s) 27, 364
Bsp143I GATC 2 cut(s) 139, 613
BspANI GGCC 3 cut(s) 105, 147, 497
BspCNI CTCAG 5 cut(s) 272, 281, 305, 372, 406
BspPI GGATC 2 cut(s) 134, 621
BsrDI GCAATG 1 cut(s) 445
BsrI ACTGG 2 cut(s) 148, 606
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 2 cut(s) 139, 613
BssT1I CCWWGG 1 cut(s) 39
Bst2UI CCWGG 1 cut(s) 552
Bst6I CTCTTC 1 cut(s) 58
BstDEI CTNAG 5 cut(s) 259, 268, 292, 380, 393
BstF5I GGATG 2 cut(s) 78, 427
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 2 cut(s) 142, 616
BstMAI GTCTC 1 cut(s) 247
BstMBI GATC 2 cut(s) 139, 613
BstMWI GCNNNNNNNGC 3 cut(s) 218, 269, 374
BstNI CCWGG 1 cut(s) 552
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 2 cut(s) 142, 550
BstSLI GKGCMC 1 cut(s) 364
BstV1I GCAGC 2 cut(s) 88, 309
BstV2I GAAGAC 1 cut(s) 4
BstX2I RGATCY 2 cut(s) 139, 613
BstYI RGATCY 2 cut(s) 139, 613
Bsu36I CCTNAGG 1 cut(s) 292
BsuRI GGCC 3 cut(s) 105, 147, 497
BtgZI GCGATG 1 cut(s) 501
BtsCI GGATG 2 cut(s) 78, 427
BtsI GCAGTG 1 cut(s) 473
BtsIMutI CAGTG 4 cut(s) 309, 377, 473, 613
CfoI GCGC 1 cut(s) 445
Cfr13I GGNCC 3 cut(s) 103, 145, 495
Csp6I GTAC 2 cut(s) 47, 633
CviAII CATG 2 cut(s) 365, 463
CviQI GTAC 2 cut(s) 47, 633
DdeI CTNAG 5 cut(s) 259, 268, 292, 380, 393
DpnI GATC 2 cut(s) 141, 615
DpnII GATC 2 cut(s) 139, 613
DraI TTTAAA 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 58
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 27
Eco32I GATATC 2 cut(s) 178, 340
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 101
Eco81I CCTNAGG 1 cut(s) 292
EcoICRI GAGCTC 1 cut(s) 25
EcoRII CCWGG 1 cut(s) 550
EcoRV GATATC 2 cut(s) 178, 340
EcoT14I CCWWGG 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 2 cut(s) 368, 466
FaiI YATR 9 cut(s) 366, 431, 464, 483, 511, 513, 582, 584, 595
FatI CATG 2 cut(s) 364, 462
Fnu4HI GCNGC 2 cut(s) 77, 298
FokI GGATG 2 cut(s) 85, 434
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 2 cut(s) 77, 298
FspI TGCGCA 1 cut(s) 444
GlaI GCGC 1 cut(s) 444
GluI GCNGC 2 cut(s) 77, 298
GsaI CCCAGC 1 cut(s) 496
HaeIII GGCC 3 cut(s) 105, 147, 497
HapII CCGG 1 cut(s) 143
HhaI GCGC 1 cut(s) 445
Hin1II CATG 2 cut(s) 368, 466
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HpaII CCGG 1 cut(s) 143
Hpy166II GTNNAC 2 cut(s) 362, 602
Hpy188I TCNGA 1 cut(s) 507
Hpy188III TCNNGA 1 cut(s) 319
Hpy8I GTNNAC 2 cut(s) 362, 602
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 1 cut(s) 537
HpyCH4V TGCA 6 cut(s) 76, 362, 368, 466, 471, 586
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 269, 374
HpyF3I CTNAG 5 cut(s) 259, 268, 292, 380, 393
Hsp92II CATG 2 cut(s) 368, 466
HspAI GCGC 1 cut(s) 443
Kzo9I GATC 2 cut(s) 139, 613
LmnI GCTCC 1 cut(s) 30
Lsp1109I GCAGC 2 cut(s) 88, 309
LweI GCATC 2 cut(s) 63, 480
MaeIII GTNAC 1 cut(s) 126
MalI GATC 2 cut(s) 141, 615
MboI GATC 2 cut(s) 139, 613
MboII GAAGA 5 cut(s) 4, 75, 94, 149, 429
MfeI CAATTG 1 cut(s) 369
MflI RGATCY 2 cut(s) 139, 613
MhlI GDGCHC 2 cut(s) 27, 364
MluCI AATT 5 cut(s) 111, 301, 369, 384, 555
MmeI TCCRAC 2 cut(s) 309, 542
MnlI CCTC 9 cut(s) 59, 211, 265, 268, 283, 301, 406, 412, 604
MseI TTAA 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 79
MspI CCGG 1 cut(s) 143
MspR9I CCNGG 2 cut(s) 144, 552
MunI CAATTG 1 cut(s) 369
Mva1269I GAATGC 1 cut(s) 263
MvaI CCWGG 1 cut(s) 552
MwoI GCNNNNNNNGC 3 cut(s) 218, 269, 374
NciI CCSGG 1 cut(s) 144
NdeII GATC 2 cut(s) 139, 613
NlaIII CATG 2 cut(s) 368, 466
NsbI TGCGCA 1 cut(s) 444
NspI RCATGY 1 cut(s) 368
PctI GAATGC 1 cut(s) 263
PkrI GCNGC 2 cut(s) 78, 299
Psp124BI GAGCTC 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 550
PspFI CCCAGC 1 cut(s) 492
PspGI CCWGG 1 cut(s) 550
PspPI GGNCC 3 cut(s) 103, 145, 495
PsuI RGATCY 2 cut(s) 139, 613
PvuII CAGCTG 1 cut(s) 79
RsaI GTAC 2 cut(s) 48, 634
RsaNI GTAC 2 cut(s) 47, 633
SacI GAGCTC 1 cut(s) 27
SaqAI TTAA 1 cut(s) 401
SatI GCNGC 2 cut(s) 77, 298
Sau3AI GATC 2 cut(s) 139, 613
Sau96I GGNCC 3 cut(s) 103, 145, 495
ScaI AGTACT 1 cut(s) 634
ScrFI CCNGG 2 cut(s) 144, 552
SduI GDGCHC 2 cut(s) 27, 364
SetI ASST 3 cut(s) 12, 27, 81
SfaNI GCATC 2 cut(s) 63, 480
SmlI CTYRAG 2 cut(s) 317, 410
SmoI CTYRAG 2 cut(s) 317, 410
Sse9I AATT 5 cut(s) 111, 301, 369, 384, 555
SstI GAGCTC 1 cut(s) 27
StyD4I CCNGG 2 cut(s) 142, 550
StyI CCWWGG 1 cut(s) 39
TaqI TCGA 2 cut(s) 116, 478
TasI AATT 5 cut(s) 111, 301, 369, 384, 555
TatI WGTACW 2 cut(s) 46, 632
Tru1I TTAA 1 cut(s) 401
Tru9I TTAA 1 cut(s) 401
TscAI CASTG 4 cut(s) 316, 384, 473, 613
TseI GCWGC 2 cut(s) 76, 297
TspDTI ATGAA 1 cut(s) 344
TspRI CASTG 4 cut(s) 316, 384, 473, 613
VneI GTGCAC 1 cut(s) 360
XceI RCATGY 1 cut(s) 368
ZrmI AGTACT 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.