RchiOBHm_Chr3g0495071

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002251
Physical Location & Seq
Reverse (-)
44404594 .. 44405375
782 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45754

Sequence Viewer

Length: 603 bp
ATGTTGCAGAGGCTTAATCCTACAAACCCTTTGAGAATTGCTATCAATGCTAAGACCATAAGCGCTGCTCCTCCTCCTCCTTATCGGTTCCAACCGGCCCAAAATGTCAGAGTTGGTACTACTTCACCTTCATCTCTATCTGATTTCTCTCAGTCTCACACAACTCCTCCTCAGCATTCTCAGCCTCGCTCTAATCCAACCCCTCAATTTGATGTTTTATGGGTTTTCGCTTTCTCTGAATTTGTTTACCAGGAAGCGATTACAACACTAAACTCAAGAGCCTACACCAACAAGATATCTTTGTTCCTTTTCATTGTGCACATGGTGGCTGCTATTGGGCTAGGTGATTCTCTATATTATCTCAGAGGAATGCAGTCCAATACTCAGTTTTGTCTCCAGAGAGCCTGGAGTATAAGTCTTGGAAGTGCTTGGTTTGGATCTCTATTTGTGCTAGTAATTGAAGCACTGAGAATTGTTGCTCGGGCTTTAAATCTACTTGCGGGCAAGGATGAGTTTATGTTTTCTTGTGCGCATTGCTTTCTCAATGTCATACAGAGCATCTTTCGATATGGCGACGGCTGGGCCTTTGTTCAGGTACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

200

Amino Acids

22.4

Weight (kDa)

9.41

Isoelectric Point (pI)

41.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 105 - 199 5.5e-09 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 531
Acc65I GGTACC 1 cut(s) 595
AccB1I GGYRCC 1 cut(s) 595
AciI CCGC 1 cut(s) 500
AclWI GGATC 1 cut(s) 445
AcsI RAATTY 1 cut(s) 239
AdeI CACNNNGTG 1 cut(s) 325
AfaI GTAC 2 cut(s) 118, 597
AfeI AGCGCT 1 cut(s) 64
AgsI TTSAA 1 cut(s) 461
AjnI CCWGG 2 cut(s) 249, 404
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 2 cut(s) 159, 398
Alw44I GTGCAC 1 cut(s) 317
AlwI GGATC 1 cut(s) 445
Ama87I CYCGRG 1 cut(s) 480
Aor51HI AGCGCT 1 cut(s) 64
AoxI GGCC 2 cut(s) 96, 582
ApaLI GTGCAC 1 cut(s) 317
ApeKI GCWGC 2 cut(s) 65, 329
ApoI RAATTY 1 cut(s) 239
Asp718I GGTACC 1 cut(s) 595
AspLEI GCGC 2 cut(s) 65, 532
AspS9I GGNCC 2 cut(s) 97, 582
AsuHPI GGTGA 2 cut(s) 117, 356
AvaI CYCGRG 1 cut(s) 480
BaeGI GKGCMC 1 cut(s) 321
BanI GGYRCC 1 cut(s) 595
Bbv12I GWGCWC 1 cut(s) 321
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 2 cut(s) 52, 316
BceAI ACGGC 1 cut(s) 592
BciT130I CCWGG 2 cut(s) 251, 406
BcoDI GTCTC 2 cut(s) 159, 398
BfaI CTAG 2 cut(s) 341, 452
BfoI RGCGCY 1 cut(s) 66
BisI GCNGC 2 cut(s) 66, 330
BlsI GCNGC 2 cut(s) 67, 331
Bme1390I CCNGG 2 cut(s) 251, 406
BmeT110I CYCGRG 1 cut(s) 480
BmgT120I GGNCC 2 cut(s) 97, 582
BmiI GGNNCC 2 cut(s) 89, 597
BmrFI CCNGG 2 cut(s) 251, 406
BmsI GCATC 1 cut(s) 567
BpmI CTGGAG 2 cut(s) 380, 427
Bpu10I CCTNAGC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 259
BsaXI ACNNNNNCTCC 4 cut(s) 151, 181, 400, 430
Bse118I RCCGGY 1 cut(s) 94
Bse3DI GCAATG 1 cut(s) 532
BseBI CCWGG 2 cut(s) 251, 406
BseGI GGATG 1 cut(s) 514
BseMI GCAATG 1 cut(s) 532
BseMII CTCAG 6 cut(s) 164, 185, 194, 376, 398, 458
BseRI GAGGAG 5 cut(s) 60, 63, 66, 156, 159
BseSI GKGCMC 1 cut(s) 321
BseXI GCAGC 2 cut(s) 52, 316
BseYI CCCAGC 1 cut(s) 579
BshFI GGCC 2 cut(s) 98, 584
BshNI GGYRCC 1 cut(s) 595
BsiHKAI GWGCWC 1 cut(s) 321
BsiHKCI CYCGRG 1 cut(s) 480
BsiSI CCGG 1 cut(s) 95
BsmAI GTCTC 2 cut(s) 159, 398
BsmI GAATGC 2 cut(s) 175, 375
BsnI GGCC 2 cut(s) 98, 584
BsoBI CYCGRG 1 cut(s) 480
Bsp1286I GDGCHC 1 cut(s) 321
Bsp143I GATC 1 cut(s) 437
BspACI CCGC 1 cut(s) 500
BspANI GGCC 2 cut(s) 98, 584
BspCNI CTCAG 6 cut(s) 163, 184, 193, 375, 397, 459
BspLI GGNNCC 2 cut(s) 89, 597
BspPI GGATC 1 cut(s) 445
BspT107I GGYRCC 1 cut(s) 595
BsrDI GCAATG 1 cut(s) 532
BsrFI RCCGGY 1 cut(s) 94
BssAI RCCGGY 1 cut(s) 94
BssMI GATC 1 cut(s) 437
Bst2UI CCWGG 2 cut(s) 251, 406
BstC8I GCNNGC 1 cut(s) 502
BstDEI CTNAG 7 cut(s) 51, 150, 171, 180, 362, 384, 467
BstF5I GGATG 1 cut(s) 514
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 2 cut(s) 65, 532
BstKTI GATC 1 cut(s) 440
BstMAI GTCTC 2 cut(s) 159, 398
BstMBI GATC 1 cut(s) 437
BstMWI GCNNNNNNNGC 2 cut(s) 47, 181
BstNI CCWGG 2 cut(s) 251, 406
BstSCI CCNGG 2 cut(s) 249, 404
BstSLI GKGCMC 1 cut(s) 321
BstV1I GCAGC 2 cut(s) 52, 316
BstX2I RGATCY 1 cut(s) 437
BstYI RGATCY 1 cut(s) 437
BsuRI GGCC 2 cut(s) 98, 584
BtsCI GGATG 1 cut(s) 514
BtsIMutI CAGTG 1 cut(s) 464
Cac8I GCNNGC 1 cut(s) 502
CfoI GCGC 2 cut(s) 65, 532
Cfr10I RCCGGY 1 cut(s) 94
Cfr13I GGNCC 2 cut(s) 97, 582
Csp6I GTAC 2 cut(s) 117, 596
CviAII CATG 1 cut(s) 322
CviQI GTAC 2 cut(s) 117, 596
DdeI CTNAG 7 cut(s) 51, 150, 171, 180, 362, 384, 467
DpnI GATC 1 cut(s) 439
DpnII GATC 1 cut(s) 437
DraI TTTAAA 1 cut(s) 489
DraIII CACNNNGTG 1 cut(s) 325
Eco32I GATATC 1 cut(s) 297
Eco47III AGCGCT 1 cut(s) 64
Eco88I CYCGRG 1 cut(s) 480
EcoRII CCWGG 2 cut(s) 249, 404
EcoRV GATATC 1 cut(s) 297
FaeI CATG 1 cut(s) 325
FaiI YATR 8 cut(s) 59, 220, 323, 355, 413, 518, 551, 570
FatI CATG 1 cut(s) 321
FauI CCCGC 1 cut(s) 493
Fnu4HI GCNGC 2 cut(s) 66, 330
FokI GGATG 1 cut(s) 521
Fsp4HI GCNGC 2 cut(s) 66, 330
FspAI RTGCGCAY 1 cut(s) 531
FspBI CTAG 2 cut(s) 341, 452
FspI TGCGCA 1 cut(s) 531
GlaI GCGC 2 cut(s) 64, 531
GluI GCNGC 2 cut(s) 66, 330
GsaI CCCAGC 1 cut(s) 583
GsuI CTGGAG 2 cut(s) 380, 427
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 2 cut(s) 98, 584
HapII CCGG 1 cut(s) 95
HhaI GCGC 2 cut(s) 65, 532
Hin1II CATG 1 cut(s) 325
Hin6I GCGC 2 cut(s) 63, 530
HinP1I GCGC 2 cut(s) 63, 530
HinfI GANTC 1 cut(s) 347
HpaII CCGG 1 cut(s) 95
HphI GGTGA 2 cut(s) 117, 356
Hpy166II GTNNAC 2 cut(s) 247, 319
Hpy188I TCNGA 4 cut(s) 110, 142, 238, 365
Hpy188III TCNNGA 2 cut(s) 276, 397
Hpy8I GTNNAC 2 cut(s) 247, 319
Hpy99I CGWCG 1 cut(s) 578
HpyAV CCTTC 1 cut(s) 138
HpyCH4V TGCA 3 cut(s) 7, 319, 373
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 181
HpyF3I CTNAG 7 cut(s) 51, 150, 171, 180, 362, 384, 467
Hsp92II CATG 1 cut(s) 325
HspAI GCGC 2 cut(s) 63, 530
KpnI GGTACC 1 cut(s) 599
Kzo9I GATC 1 cut(s) 437
LmnI GCTCC 1 cut(s) 73
LpnPI CCDG 8 cut(s) 108, 236, 263, 391, 410, 418, 565, 578
Lsp1109I GCAGC 2 cut(s) 52, 316
LweI GCATC 1 cut(s) 567
MaeI CTAG 2 cut(s) 341, 452
MalI GATC 1 cut(s) 439
MboI GATC 1 cut(s) 437
MflI RGATCY 1 cut(s) 437
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 5 cut(s) 36, 206, 239, 456, 471
MmeI TCCRAC 2 cut(s) 115, 221
MnlI CCTC 9 cut(s) 3, 81, 84, 87, 177, 180, 195, 213, 359
MseI TTAA 3 cut(s) 15, 488, 601
MspI CCGG 1 cut(s) 95
MspR9I CCNGG 2 cut(s) 251, 406
Mva1269I GAATGC 2 cut(s) 175, 375
MvaI CCWGG 2 cut(s) 251, 406
MwoI GCNNNNNNNGC 2 cut(s) 47, 181
NdeII GATC 1 cut(s) 437
NlaIII CATG 1 cut(s) 325
NlaIV GGNNCC 2 cut(s) 89, 597
NsbI TGCGCA 1 cut(s) 531
PctI GAATGC 2 cut(s) 175, 375
PfeI GAWTC 1 cut(s) 347
PkrI GCNGC 2 cut(s) 67, 331
Psp6I CCWGG 2 cut(s) 249, 404
PspFI CCCAGC 1 cut(s) 579
PspGI CCWGG 2 cut(s) 249, 404
PspN4I GGNNCC 2 cut(s) 89, 597
PspPI GGNCC 2 cut(s) 97, 582
PsuI RGATCY 1 cut(s) 437
RsaI GTAC 2 cut(s) 118, 597
RsaNI GTAC 2 cut(s) 117, 596
SaqAI TTAA 3 cut(s) 15, 488, 601
SatI GCNGC 2 cut(s) 66, 330
Sau3AI GATC 1 cut(s) 437
Sau96I GGNCC 2 cut(s) 97, 582
ScrFI CCNGG 2 cut(s) 251, 406
SduI GDGCHC 1 cut(s) 321
SetI ASST 4 cut(s) 130, 346, 597, 601
SfaNI GCATC 1 cut(s) 567
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
Sse9I AATT 5 cut(s) 36, 206, 239, 456, 471
SsiI CCGC 1 cut(s) 500
SspMI CTAG 2 cut(s) 341, 452
StyD4I CCNGG 2 cut(s) 249, 404
TaqI TCGA 1 cut(s) 565
TasI AATT 5 cut(s) 36, 206, 239, 456, 471
TfiI GAWTC 1 cut(s) 347
Tru1I TTAA 3 cut(s) 15, 488, 601
Tru9I TTAA 3 cut(s) 15, 488, 601
TscAI CASTG 1 cut(s) 471
TseI GCWGC 2 cut(s) 65, 329
TspDTI ATGAA 2 cut(s) 120, 301
TspRI CASTG 1 cut(s) 471
VneI GTGCAC 1 cut(s) 317
XapI RAATTY 1 cut(s) 239
XspI CTAG 2 cut(s) 341, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.