Rh1AG033300

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0002251
Physical Location & Seq
Reverse (-)
5938203 .. 5942066
3864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG033300.1

Sequence Viewer

Length: 1257 bp
ATGGTCGGCGCTGTTGGGCTAGTTGGTTTTCTGACATTCAAGGGAGTTCAGGGTCTGATACAAGGTTCAGGCAGTATTTCGAGAAAGGAGAAAAGGGTGCTAAAGTATTTTCTACCACAAGTGGAAGCTGCATCTCTTATGAGCATTACTCTTGCATTTGCATGGCAAAAGGCATTTAGGAAATGGCCTACATTTATGGTTCATTTCATACTATGGAACACTTTTGTCATGAGTTTATCTGCTGGAATCCTACTAATTTGTTTCCAAAAGCCCACCACTACTGGCGTGGGAGTCTGTTTCATTGGCTTTGCCATAGGGAATAGCTTGTACGCTTGTTGGATCACGCCGCGGATAGGATTTTGTATCAGGATATTGATCAAGTCGCTCGACCCTGTATCAAAGTTCCGTGATTTGAATCAACCCACTTACTGGATGCTTGGGTTCGGATTTTTGTGGATGTCTCTATGGATTTTAGGAGTCATTGGAGCCTTGAATTTCTACTTTCCTCCACTAGTCATAATTTCATTGGTATTGAGCCTGATGTGGACAGCCGAAGTAATGAGGAATGTAGTTAATATAACTGTGAGTAGGGTGATTGCTCTATATTATCTCAGAGGAATGCAGTCCAATACTCAGTTTTGTTTCCAGAGAGCCTTGAGTAAAAATCTTGGAAGTGCTTGTTTTGGATCTCTGTTTGTGCCAGCAATTGAAGCGCTGAGAATTGTTGCTCGAGCTTTAAATCTACTTGAGGGAGAGGATGAGTTCATGTTTTCTTGTGCGCATTGCTGTCTCCATGTCATGCAGTGCATCTTCCGATATGGCAATGGCTGGGCCTTTGTTCAGATAGCAGCATATGGAAAAGATTTTGTGAGGGCATCACAAGAGACTTGGGAGCTCTTTGAAAGGCAAGAAATTCATCTCCTTGTTGATTCTGACATCACCAGCTCAATATGCTTCCTAACCGGCATCTGCAGTGGCTCTATGTGTGTCATAGTTGTGGCTGCTTGGACCGCCACAGTACACCAAAGTTTCACGGCCACCATTTCCCTGCTTGCATTCTTCATTGGATACCTTATGACCAGGATCGCAATGGCACTACCACATGCCTGTGTAGGATGTTACTACGCATGCTATGCAGAGAATCCAGACAACAGATTATTTGATGACACAATAAAGGATCGTCTTGCTCTGTTGAAATCTCGACCAGATGTACTCGTCCCCACACCTCGAGTCCCTCGCCGGTTCGCAGCAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

418

Amino Acids

46.91

Weight (kDa)

9.12

Isoelectric Point (pI)

34.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 99 - 383 2.9e-38 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 780
AccB7I CCANNNNNTGG 1 cut(s) 429
AccII CGCG 1 cut(s) 349
AciI CCGC 3 cut(s) 347, 349, 1011
AclWI GGATC 4 cut(s) 347, 694, 1091, 1185
AcoI YGGCCR 1 cut(s) 1035
AcsI RAATTY 2 cut(s) 493, 912
AfaI GTAC 3 cut(s) 329, 1020, 1212
AfeI AGCGCT 1 cut(s) 714
AfiI CCNNNNNNNGG 2 cut(s) 353, 429
AgsI TTSAA 6 cut(s) 40, 415, 493, 710, 902, 1195
AhlI ACTAGT 1 cut(s) 511
AjnI CCWGG 1 cut(s) 1079
AluBI AGCT 5 cut(s) 128, 324, 734, 895, 945
AluI AGCT 5 cut(s) 128, 324, 734, 895, 945
Alw21I GWGCWC 1 cut(s) 897
Alw26I GTCTC 3 cut(s) 465, 794, 878
AlwI GGATC 4 cut(s) 347, 694, 1091, 1185
AlwNI CAGNNNCTG 1 cut(s) 55
Ama87I CYCGRG 2 cut(s) 729, 1227
Aor51HI AGCGCT 1 cut(s) 714
AoxI GGCC 3 cut(s) 185, 831, 1035
ApeKI GCWGC 4 cut(s) 128, 848, 1001, 1247
ApoI RAATTY 2 cut(s) 493, 912
AspLEI GCGC 3 cut(s) 11, 715, 781
AspS9I GGNCC 2 cut(s) 831, 1008
AsuHPI GGTGA 2 cut(s) 604, 931
AvaI CYCGRG 2 cut(s) 729, 1227
AvaII GGWCC 1 cut(s) 1008
BanII GRGCYC 1 cut(s) 897
Bbv12I GWGCWC 1 cut(s) 897
BbvI GCAGC 3 cut(s) 115, 860, 988
BceAI ACGGC 1 cut(s) 1050
BciT130I CCWGG 1 cut(s) 1081
BciVI GTATCC 1 cut(s) 1061
BclI TGATCA 1 cut(s) 375
BcoDI GTCTC 3 cut(s) 465, 794, 878
BcuI ACTAGT 1 cut(s) 511
BfaI CTAG 2 cut(s) 20, 512
BfmI CTRYAG 1 cut(s) 970
BfoI RGCGCY 2 cut(s) 12, 716
BfuI GTATCC 1 cut(s) 1061
BisI GCNGC 5 cut(s) 129, 347, 849, 1002, 1248
BlsI GCNGC 5 cut(s) 130, 348, 850, 1003, 1249
Bme1390I CCNGG 1 cut(s) 1081
Bme18I GGWCC 1 cut(s) 1008
BmeT110I CYCGRG 2 cut(s) 729, 1227
BmgT120I GGNCC 2 cut(s) 831, 1008
BmiI GGNNCC 1 cut(s) 487
BmrFI CCNGG 1 cut(s) 1081
BmsI GCATC 5 cut(s) 140, 423, 816, 884, 975
BplI GAGNNNNNCTC 2 cut(s) 133, 165
BpuEI CTTGAG 2 cut(s) 676, 767
BsaBI GATNNNNATC 2 cut(s) 374, 414
BsaJI CCNNGG 1 cut(s) 347
Bsc4I CCNNNNNNNGG 2 cut(s) 353, 429
Bse118I RCCGGY 2 cut(s) 962, 1239
Bse1I ACTGG 2 cut(s) 286, 434
Bse3DI GCAATG 3 cut(s) 781, 829, 1095
Bse8I GATNNNNATC 2 cut(s) 374, 414
BseBI CCWGG 1 cut(s) 1081
BseDI CCNNGG 1 cut(s) 347
BseGI GGATG 4 cut(s) 438, 462, 763, 1121
BseJI GATNNNNATC 2 cut(s) 374, 414
BseLI CCNNNNNNNGG 2 cut(s) 353, 429
BseMI GCAATG 3 cut(s) 781, 829, 1095
BseMII CTCAG 3 cut(s) 625, 647, 707
BseNI ACTGG 2 cut(s) 286, 434
BseXI GCAGC 3 cut(s) 115, 860, 988
BseYI CCCAGC 1 cut(s) 828
Bsh1236I CGCG 1 cut(s) 349
BshFI GGCC 3 cut(s) 187, 833, 1037
BsiHKAI GWGCWC 1 cut(s) 897
BsiHKCI CYCGRG 2 cut(s) 729, 1227
BsiSI CCGG 2 cut(s) 963, 1240
BslFI GGGAC 2 cut(s) 1202, 1217
BslI CCNNNNNNNGG 2 cut(s) 353, 429
BsmAI GTCTC 3 cut(s) 465, 794, 878
BsmFI GGGAC 2 cut(s) 1202, 1217
BsmI GAATGC 2 cut(s) 624, 1055
BsnI GGCC 3 cut(s) 187, 833, 1037
BsoBI CYCGRG 2 cut(s) 729, 1227
Bsp1286I GDGCHC 1 cut(s) 897
Bsp143I GATC 5 cut(s) 339, 375, 686, 1083, 1177
BspACI CCGC 3 cut(s) 347, 349, 1011
BspANI GGCC 3 cut(s) 187, 833, 1037
BspCNI CTCAG 3 cut(s) 624, 646, 708
BspFNI CGCG 1 cut(s) 349
BspHI TCATGA 1 cut(s) 228
BspLI GGNNCC 1 cut(s) 487
BspMAI CTGCAG 1 cut(s) 974
BspPI GGATC 4 cut(s) 347, 694, 1091, 1185
BsrDI GCAATG 3 cut(s) 781, 829, 1095
BsrFI RCCGGY 2 cut(s) 962, 1239
BsrI ACTGG 2 cut(s) 286, 434
BssAI RCCGGY 2 cut(s) 962, 1239
BssECI CCNNGG 1 cut(s) 347
BssMI GATC 5 cut(s) 339, 375, 686, 1083, 1177
Bst2UI CCWGG 1 cut(s) 1081
Bst4CI ACNGT 2 cut(s) 583, 1018
BstAPI GCANNNNNTGC 1 cut(s) 1133
BstC8I GCNNGC 3 cut(s) 702, 1053, 1129
BstDEI CTNAG 3 cut(s) 611, 633, 716
BstDSI CCRYGG 1 cut(s) 347
BstF5I GGATG 4 cut(s) 438, 462, 763, 1121
BstFNI CGCG 1 cut(s) 349
BstH2I RGCGCY 2 cut(s) 12, 716
BstHHI GCGC 3 cut(s) 11, 715, 781
BstKTI GATC 5 cut(s) 342, 378, 689, 1086, 1180
BstMAI GTCTC 3 cut(s) 465, 794, 878
BstMBI GATC 5 cut(s) 339, 375, 686, 1083, 1177
BstMWI GCNNNNNNNGC 4 cut(s) 710, 951, 1010, 1133
BstNI CCWGG 1 cut(s) 1081
BstNSI RCATGY 2 cut(s) 1106, 1131
BstSCI CCNGG 1 cut(s) 1079
BstSFI CTRYAG 1 cut(s) 970
BstUI CGCG 1 cut(s) 349
BstV1I GCAGC 3 cut(s) 115, 860, 988
BstX2I RGATCY 1 cut(s) 686
BstYI RGATCY 1 cut(s) 686
BsuI GTATCC 1 cut(s) 1061
BsuRI GGCC 3 cut(s) 187, 833, 1037
BtgI CCRYGG 1 cut(s) 347
BtsCI GGATG 4 cut(s) 438, 462, 763, 1121
BtsI GCAGTG 2 cut(s) 809, 979
BtsIMutI CAGTG 2 cut(s) 809, 979
Cac8I GCNNGC 3 cut(s) 702, 1053, 1129
CaiI CAGNNNCTG 1 cut(s) 55
CciI TCATGA 1 cut(s) 228
CfoI GCGC 3 cut(s) 11, 715, 781
Cfr10I RCCGGY 2 cut(s) 962, 1239
Cfr13I GGNCC 2 cut(s) 831, 1008
Cfr42I CCGCGG 1 cut(s) 350
Csp6I GTAC 3 cut(s) 328, 1019, 1211
CviAII CATG 7 cut(s) 162, 229, 766, 794, 799, 1103, 1128
CviQI GTAC 3 cut(s) 328, 1019, 1211
DdeI CTNAG 3 cut(s) 611, 633, 716
DpnI GATC 5 cut(s) 341, 377, 688, 1085, 1179
DpnII GATC 5 cut(s) 339, 375, 686, 1083, 1177
DraI TTTAAA 1 cut(s) 738
EaeI YGGCCR 1 cut(s) 1035
Ecl136II GAGCTC 1 cut(s) 895
Eco24I GRGCYC 1 cut(s) 897
Eco47I GGWCC 1 cut(s) 1008
Eco47III AGCGCT 1 cut(s) 714
Eco53kI GAGCTC 1 cut(s) 895
Eco88I CYCGRG 2 cut(s) 729, 1227
EcoICRI GAGCTC 1 cut(s) 895
EcoRII CCWGG 1 cut(s) 1079
EcoT38I GRGCYC 1 cut(s) 897
FaeI CATG 7 cut(s) 165, 232, 769, 797, 802, 1106, 1131
FaqI GGGAC 2 cut(s) 1202, 1217
FatI CATG 7 cut(s) 161, 228, 765, 793, 798, 1102, 1127
FauNDI CATATG 1 cut(s) 853
FbaI TGATCA 1 cut(s) 375
Fnu4HI GCNGC 5 cut(s) 129, 347, 849, 1002, 1248
FokI GGATG 4 cut(s) 445, 469, 770, 1128
FriOI GRGCYC 1 cut(s) 897
Fsp4HI GCNGC 5 cut(s) 129, 347, 849, 1002, 1248
FspAI RTGCGCAY 1 cut(s) 780
FspBI CTAG 2 cut(s) 20, 512
FspI TGCGCA 1 cut(s) 780
GlaI GCGC 3 cut(s) 10, 714, 780
GluI GCNGC 5 cut(s) 129, 347, 849, 1002, 1248
GsaI CCCAGC 1 cut(s) 832
HaeII RGCGCY 2 cut(s) 12, 716
HaeIII GGCC 3 cut(s) 187, 833, 1037
HapII CCGG 2 cut(s) 963, 1240
HhaI GCGC 3 cut(s) 11, 715, 781
Hin1II CATG 7 cut(s) 165, 232, 769, 797, 802, 1106, 1131
Hin6I GCGC 3 cut(s) 9, 713, 779
HinP1I GCGC 3 cut(s) 9, 713, 779
HinfI GANTC 7 cut(s) 246, 291, 415, 477, 929, 1141, 1230
HpaII CCGG 2 cut(s) 963, 1240
HphI GGTGA 2 cut(s) 604, 931
Hpy166II GTNNAC 2 cut(s) 546, 1021
Hpy188I TCNGA 7 cut(s) 33, 57, 446, 614, 815, 843, 934
Hpy188III TCNNGA 6 cut(s) 81, 229, 367, 646, 1145, 1200
Hpy8I GTNNAC 2 cut(s) 546, 1021
HpyCH4III ACNGT 2 cut(s) 583, 1018
HpyCH4V TGCA 9 cut(s) 131, 155, 161, 622, 802, 807, 972, 1055, 1136
HpyF10VI GCNNNNNNNGC 4 cut(s) 710, 951, 1010, 1133
HpyF3I CTNAG 3 cut(s) 611, 633, 716
Hsp92II CATG 7 cut(s) 165, 232, 769, 797, 802, 1106, 1131
HspAI GCGC 3 cut(s) 9, 713, 779
Ksp22I TGATCA 1 cut(s) 375
KspI CCGCGG 1 cut(s) 350
Kzo9I GATC 5 cut(s) 339, 375, 686, 1083, 1177
LmnI GCTCC 2 cut(s) 485, 892
Lsp1109I GCAGC 3 cut(s) 115, 860, 988
LweI GCATC 5 cut(s) 140, 423, 816, 884, 975
MaeI CTAG 2 cut(s) 20, 512
MaeIII GTNAC 1 cut(s) 1118
MalI GATC 5 cut(s) 341, 377, 688, 1085, 1179
MboI GATC 5 cut(s) 339, 375, 686, 1083, 1177
MboII GAAGA 2 cut(s) 802, 1051
MfeI CAATTG 1 cut(s) 705
MflI RGATCY 1 cut(s) 686
MhlI GDGCHC 1 cut(s) 897
MluCI AATT 6 cut(s) 255, 493, 519, 705, 720, 912
MlyI GAGTC 3 cut(s) 300, 486, 1239
MmeI TCCRAC 1 cut(s) 317
MnlI CCTC 8 cut(s) 516, 555, 608, 742, 748, 864, 1236, 1245
MseI TTAA 2 cut(s) 573, 737
MslI CAYNNNNRTG 3 cut(s) 160, 995, 1107
MspA1I CMGCKG 1 cut(s) 349
MspI CCGG 2 cut(s) 963, 1240
MspR9I CCNGG 1 cut(s) 1081
MunI CAATTG 1 cut(s) 705
Mva1269I GAATGC 2 cut(s) 624, 1055
MvaI CCWGG 1 cut(s) 1081
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 4 cut(s) 710, 951, 1010, 1133
NdeI CATATG 1 cut(s) 853
NdeII GATC 5 cut(s) 339, 375, 686, 1083, 1177
NlaIII CATG 7 cut(s) 165, 232, 769, 797, 802, 1106, 1131
NlaIV GGNNCC 1 cut(s) 487
NsbI TGCGCA 1 cut(s) 780
NspI RCATGY 2 cut(s) 1106, 1131
PaeI GCATGC 1 cut(s) 1131
PaeR7I CTCGAG 2 cut(s) 729, 1227
PagI TCATGA 1 cut(s) 228
PctI GAATGC 2 cut(s) 624, 1055
PfeI GAWTC 4 cut(s) 246, 415, 929, 1141
PflMI CCANNNNNTGG 1 cut(s) 429
PkrI GCNGC 5 cut(s) 130, 348, 850, 1003, 1249
PleI GAGTC 3 cut(s) 299, 485, 1238
PpsI GAGTC 3 cut(s) 299, 485, 1238
Psp124BI GAGCTC 1 cut(s) 897
Psp6I CCWGG 1 cut(s) 1079
PspFI CCCAGC 1 cut(s) 828
PspGI CCWGG 1 cut(s) 1079
PspN4I GGNNCC 1 cut(s) 487
PspPI GGNCC 2 cut(s) 831, 1008
PspXI VCTCGAGB 2 cut(s) 729, 1227
PstI CTGCAG 1 cut(s) 974
PstNI CAGNNNCTG 1 cut(s) 55
PsuI RGATCY 1 cut(s) 686
RsaI GTAC 3 cut(s) 329, 1020, 1212
RsaNI GTAC 3 cut(s) 328, 1019, 1211
RseI CAYNNNNRTG 3 cut(s) 160, 995, 1107
SacI GAGCTC 1 cut(s) 897
SacII CCGCGG 1 cut(s) 350
SaqAI TTAA 2 cut(s) 573, 737
SatI GCNGC 5 cut(s) 129, 347, 849, 1002, 1248
Sau3AI GATC 5 cut(s) 339, 375, 686, 1083, 1177
Sau96I GGNCC 2 cut(s) 831, 1008
SchI GAGTC 3 cut(s) 300, 486, 1239
ScrFI CCNGG 1 cut(s) 1081
SduI GDGCHC 1 cut(s) 897
SetI ASST 9 cut(s) 67, 130, 326, 736, 897, 947, 1074, 1228, 1256
SfaNI GCATC 5 cut(s) 140, 423, 816, 884, 975
SfcI CTRYAG 1 cut(s) 970
Sfr274I CTCGAG 2 cut(s) 729, 1227
Sfr303I CCGCGG 1 cut(s) 350
SgrBI CCGCGG 1 cut(s) 350
SinI GGWCC 1 cut(s) 1008
SlaI CTCGAG 2 cut(s) 729, 1227
SmiMI CAYNNNNRTG 3 cut(s) 160, 995, 1107
SmlI CTYRAG 4 cut(s) 655, 729, 746, 1227
SmoI CTYRAG 4 cut(s) 655, 729, 746, 1227
SpeI ACTAGT 1 cut(s) 511
SphI GCATGC 1 cut(s) 1131
Sse9I AATT 6 cut(s) 255, 493, 519, 705, 720, 912
SsiI CCGC 3 cut(s) 347, 349, 1011
SspMI CTAG 2 cut(s) 20, 512
SstI GAGCTC 1 cut(s) 897
StyD4I CCNGG 1 cut(s) 1079
TaaI ACNGT 2 cut(s) 583, 1018
TaqI TCGA 5 cut(s) 80, 387, 730, 1201, 1228
TasI AATT 6 cut(s) 255, 493, 519, 705, 720, 912
TatI WGTACW 2 cut(s) 1018, 1210
TauI GCSGC 1 cut(s) 349
TfiI GAWTC 4 cut(s) 246, 415, 929, 1141
Tru1I TTAA 2 cut(s) 573, 737
Tru9I TTAA 2 cut(s) 573, 737
TscAI CASTG 2 cut(s) 809, 979
TseI GCWGC 4 cut(s) 128, 848, 1001, 1247
TspDTI ATGAA 7 cut(s) 191, 196, 289, 513, 754, 905, 1051
TspGWI ACGGA 1 cut(s) 395
TspRI CASTG 2 cut(s) 809, 979
Van91I CCANNNNNTGG 1 cut(s) 429
VpaK11BI GGWCC 1 cut(s) 1008
XapI RAATTY 2 cut(s) 493, 912
XceI RCATGY 2 cut(s) 1106, 1131
XcmI CCANNNNNNNNNTGG 2 cut(s) 283, 1087
XhoI CTCGAG 2 cut(s) 729, 1227
XspI CTAG 2 cut(s) 20, 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.