MD07G1024500.v1.1

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002251
Physical Location & Seq
Reverse (-)
2046172 .. 2049827
3656 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1024500.v1.1.491

Sequence Viewer

Length: 1764 bp
ATGGGTGCCGCAGAGCCTGTGGAGGAGAGAGAAAGAGAGCAGCTTGAGGAGAGAGAGGACTATGGGAGGAAGGAGGAGGAGGAGGATGTGAGAGATGTTGAGGAAGGGGAGGTGGGTTTTGAAGAACAGGCGGTGGCAGCTGAGAGAAACATTAATATTAATACTAATCATGGAAATAATAATATCAATCAGAATGAGAGTGAAGGAGATGTTCATATGTCAATGCTGCAGAGGCTGAATCCAACAAACCCTTTGAGAATTGTAATAAATAGCAAAACCAGAGCTGCAACTCCGCCGCCGCCGCCGGCTCGGTTCTCTAATATCAGAGGCTTTGGTGGTGGTCATGGTGGTGCTACTGCTACTCCTTCCCCTCCTCAGTTCGCCTCTCACACCAACACTCCCCAGCATTCTCAGCCTCGTTCTACACCAACCCCACAACCATCTTTTACAACACTGAATTCAAGAAAATACACCAACAAGATGTCTCTGTTTCTGTTTCTTCTGCACATGGTTGCAGCCATTGGGCTAGTCGGTTTCCTTCTGTTCAAGGGAGTTCAAGGGCTGATAGAAGCATCAGACAACAAGGTTAAAAGAGCCGAGAAACGAGTGTTGAAGTTTTTTCTACCACAAGTCGAAGCTGCATCTCTTTTAAGCATTGTTCTTGCATTTGCATGGCAAAAGGCAGTCAGGCTATGGCCTAAGTTTTTTATTCATTTCATACTATGGACCACTTTTGTCATGTCTCTATCTGCCGGAATTCTGCTAATTTGCTTCCAAAAGCCTCCCACCGATGGCGTTGCAGTCTGCTTTATTGCATTTGCGATAGGCAATGGCTTATACGCTTGTTGGATCACACAGCGGATAGGATTTTGTAGCAAGATATTGATCAAATCGCTCGAACCCGTATCAAAGTTCCCCGATTTGAATCAACCCACTTATTGGATGCTTGGGGTCGGATTTCTGTGGATGTCCCTGTTGATTCTAGCTGTGATAGGAGCCTTGAATTTCTACTTTCCTCCACTGATCATAATTTTGTTGGTCTTGAGCTTAGCTTGGATAGCTGAAGTAATGAGGAACGTTGCAAATATGACCGTCAGTAGGGTAATTTCTCTTTATTATCTCAGAGGAATGCAATCTAATACTCAATTTTGCTTCCAGAGAGCCTTGAGTAAAAATCTTGGAAGTGCTTGTTTGGGTTCACTCTTTGTACCTTCAATTGAAGCACTGAGAATTGTTGCTCGGGCTTTAAATTTACTCAAGGGAGAAGATGAGTTCATGTTTTCTTGTGCACATTGCTGTCTCAAAGTCATGGAGTGCATCTTCAGATATGGCAACGGCTGGGCCTTCATACAGATAGCAGTGTATGGGAAAGGTTTTGTGAGGGCATCACAAGACACCTGGGAGCTCTTTGAACGGCAAGAAATGGAACCAATTGTTGATTCCGACATTACCAGCTCAATCTGCTTCCTAACCGGCGTCTGCAGCGGCTCTATCTGTGTCATAATTGTGGCGGCTTGGACTGCTAGAGTGCACCAAGGTTTCACAGCCACCATTTCCCTCCTTACATTCCTCATTGGATACCTTATGACGAGGATAGCCATGGCACTACCACATGCTTGTGTAGGTAGTTACTTTGTCTGCTATGCAGAGAATCCAGACAACAGATTGTTTGACAACACAATCAAGGATCGCCTCGCTTTGATGAAATCTGGTCGAGATGTAGTCGTTCCCACGCCTCGAGTCCCCCGCCGGTTTGCAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

588

Amino Acids

65.58

Weight (kDa)

8.65

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 269 - 553 5.3e-41 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 5
AccB7I CCANNNNNTGG 1 cut(s) 939
AclI AACGTT 1 cut(s) 1077
AclWI GGATC 2 cut(s) 857, 1695
AcsI RAATTY 4 cut(s) 457, 756, 1003, 1249
AcuI CTGAAG 2 cut(s) 1083, 1306
AcyI GRCGYC 1 cut(s) 1476
AfaI GTAC 1 cut(s) 1209
AfiI CCNNNNNNNGG 3 cut(s) 791, 939, 1098
AjnI CCWGG 1 cut(s) 1397
AjuI GAANNNNNNNTTGG 2 cut(s) 1423, 1455
Alw21I GWGCWC 3 cut(s) 1291, 1407, 1533
Alw26I GTCTC 3 cut(s) 489, 747, 1304
Alw44I GTGCAC 2 cut(s) 1287, 1529
AlwI GGATC 2 cut(s) 857, 1695
AlwNI CAGNNNCTG 2 cut(s) 17, 235
Ama87I CYCGRG 2 cut(s) 1239, 1737
AoxI GGCC 2 cut(s) 695, 1341
ApaLI GTGCAC 2 cut(s) 1287, 1529
ApeKI GCWGC 7 cut(s) 40, 137, 226, 284, 515, 638, 1482
ApoI RAATTY 4 cut(s) 457, 756, 1003, 1249
AseI ATTAAT 2 cut(s) 153, 159
AspS9I GGNCC 2 cut(s) 726, 1341
AvaI CYCGRG 2 cut(s) 1239, 1737
AvaII GGWCC 1 cut(s) 726
BaeGI GKGCMC 2 cut(s) 1291, 1533
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 1407
Bbv12I GWGCWC 3 cut(s) 1291, 1407, 1533
BbvI GCAGC 7 cut(s) 52, 149, 213, 271, 527, 625, 1494
BccI CCATC 2 cut(s) 448, 785
BceAI ACGGC 2 cut(s) 1351, 1430
BcgI CGANNNNNNTGC 2 cut(s) 779, 813
BciT130I CCWGG 1 cut(s) 1399
BciVI GTATCC 1 cut(s) 1571
BclI TGATCA 2 cut(s) 885, 1023
BcoDI GTCTC 3 cut(s) 489, 747, 1304
BfaI CTAG 3 cut(s) 527, 983, 1524
BfmI CTRYAG 2 cut(s) 227, 1480
BfuI GTATCC 1 cut(s) 1571
BlpI GCTNAGC 1 cut(s) 1048
Bme1390I CCNGG 1 cut(s) 1399
Bme18I GGWCC 1 cut(s) 726
BmeT110I CYCGRG 2 cut(s) 1239, 1737
BmgT120I GGNCC 2 cut(s) 726, 1341
BmiI GGNNCC 3 cut(s) 7, 997, 1428
BmrFI CCNGG 1 cut(s) 1399
BmsI GCATC 5 cut(s) 581, 650, 933, 1326, 1394
Bpu1102I GCTNAGC 1 cut(s) 1048
BpuEI CTTGAG 4 cut(s) 65, 1063, 1186, 1241
BsaBI GATNNNNATC 2 cut(s) 884, 924
BsaHI GRCGYC 1 cut(s) 1476
BsaJI CCNNGG 3 cut(s) 1398, 1534, 1599
BsaXI ACNNNNNCTCC 4 cut(s) 346, 376, 382, 412
Bsc4I CCNNNNNNNGG 3 cut(s) 791, 939, 1098
Bse118I RCCGGY 3 cut(s) 304, 1472, 1749
Bse3DI GCAATG 2 cut(s) 835, 1291
Bse8I GATNNNNATC 2 cut(s) 884, 924
BseBI CCWGG 1 cut(s) 1399
BseDI CCNNGG 3 cut(s) 1398, 1534, 1599
BseGI GGATG 3 cut(s) 91, 948, 972
BseJI GATNNNNATC 2 cut(s) 884, 924
BseLI CCNNNNNNNGG 3 cut(s) 791, 939, 1098
BseMI GCAATG 2 cut(s) 835, 1291
BseMII CTCAG 5 cut(s) 132, 389, 425, 1135, 1217
BseRI GAGGAG 6 cut(s) 38, 62, 89, 92, 95, 363
BseSI GKGCMC 2 cut(s) 1291, 1533
BseXI GCAGC 7 cut(s) 52, 149, 213, 271, 527, 625, 1494
BseYI CCCAGC 2 cut(s) 402, 1338
BsgI GTGCAG 1 cut(s) 488
BshFI GGCC 2 cut(s) 697, 1343
BshNI GGYRCC 1 cut(s) 5
BsiHKAI GWGCWC 3 cut(s) 1291, 1407, 1533
BsiHKCI CYCGRG 2 cut(s) 1239, 1737
BsiSI CCGG 4 cut(s) 305, 753, 1473, 1750
BslFI GGGAC 2 cut(s) 955, 1727
BslI CCNNNNNNNGG 3 cut(s) 791, 939, 1098
BsmAI GTCTC 3 cut(s) 489, 747, 1304
BsmFI GGGAC 2 cut(s) 955, 1727
BsmI GAATGC 2 cut(s) 406, 1134
BsnI GGCC 2 cut(s) 697, 1343
BsoBI CYCGRG 2 cut(s) 1239, 1737
Bsp1286I GDGCHC 3 cut(s) 1291, 1407, 1533
Bsp143I GATC 4 cut(s) 849, 885, 1023, 1687
Bsp1720I GCTNAGC 1 cut(s) 1048
Bsp19I CCATGG 1 cut(s) 1599
BspANI GGCC 2 cut(s) 697, 1343
BspCNI CTCAG 5 cut(s) 133, 388, 424, 1134, 1218
BspLI GGNNCC 3 cut(s) 7, 997, 1428
BspMAI CTGCAG 2 cut(s) 231, 1484
BspPI GGATC 2 cut(s) 857, 1695
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 2 cut(s) 835, 1291
BsrFI RCCGGY 3 cut(s) 304, 1472, 1749
BssAI RCCGGY 3 cut(s) 304, 1472, 1749
BssECI CCNNGG 3 cut(s) 1398, 1534, 1599
BssMI GATC 4 cut(s) 849, 885, 1023, 1687
BssNI GRCGYC 1 cut(s) 1476
BssT1I CCWWGG 2 cut(s) 1534, 1599
Bst2UI CCWGG 1 cut(s) 1399
Bst4CI ACNGT 1 cut(s) 1093
BstACI GRCGYC 1 cut(s) 1476
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 7 cut(s) 141, 375, 411, 699, 1048, 1121, 1226
BstDSI CCRYGG 1 cut(s) 1599
BstF5I GGATG 3 cut(s) 91, 948, 972
BstKTI GATC 4 cut(s) 852, 888, 1026, 1690
BstMAI GTCTC 3 cut(s) 489, 747, 1304
BstMBI GATC 4 cut(s) 849, 885, 1023, 1687
BstMWI GCNNNNNNNGC 8 cut(s) 137, 232, 301, 412, 1058, 1461, 1482, 1520
BstNI CCWGG 1 cut(s) 1399
BstNSI RCATGY 1 cut(s) 1616
BstSCI CCNGG 1 cut(s) 1397
BstSFI CTRYAG 2 cut(s) 227, 1480
BstSLI GKGCMC 2 cut(s) 1291, 1533
BstV1I GCAGC 7 cut(s) 52, 149, 213, 271, 527, 625, 1494
BsuI GTATCC 1 cut(s) 1571
BsuRI GGCC 2 cut(s) 697, 1343
BtgI CCRYGG 1 cut(s) 1599
BtsCI GGATG 3 cut(s) 91, 948, 972
BtsI GCAGTG 1 cut(s) 1365
BtsIMutI CAGTG 4 cut(s) 452, 1019, 1223, 1365
Cac8I GCNNGC 1 cut(s) 306
CaiI CAGNNNCTG 2 cut(s) 17, 235
Cfr10I RCCGGY 3 cut(s) 304, 1472, 1749
Cfr13I GGNCC 2 cut(s) 726, 1341
CseI GACGC 1 cut(s) 1465
Csp6I GTAC 1 cut(s) 1208
CviAII CATG 9 cut(s) 170, 344, 508, 672, 739, 1276, 1309, 1600, 1613
CviQI GTAC 1 cut(s) 1208
DdeI CTNAG 7 cut(s) 141, 375, 411, 699, 1048, 1121, 1226
DpnI GATC 4 cut(s) 851, 887, 1025, 1689
DpnII GATC 4 cut(s) 849, 885, 1023, 1687
DraI TTTAAA 1 cut(s) 1248
EciI GGCGGA 1 cut(s) 282
Ecl136II GAGCTC 1 cut(s) 1405
Eco130I CCWWGG 2 cut(s) 1534, 1599
Eco24I GRGCYC 1 cut(s) 1407
Eco47I GGWCC 1 cut(s) 726
Eco53kI GAGCTC 1 cut(s) 1405
Eco57I CTGAAG 2 cut(s) 1083, 1306
Eco88I CYCGRG 2 cut(s) 1239, 1737
EcoICRI GAGCTC 1 cut(s) 1405
EcoRI GAATTC 2 cut(s) 457, 756
EcoRII CCWGG 1 cut(s) 1397
EcoT14I CCWWGG 2 cut(s) 1534, 1599
EcoT38I GRGCYC 1 cut(s) 1407
ErhI CCWWGG 2 cut(s) 1534, 1599
FaeI CATG 9 cut(s) 173, 347, 511, 675, 742, 1279, 1312, 1603, 1616
FaqI GGGAC 2 cut(s) 955, 1727
FatI CATG 9 cut(s) 169, 343, 507, 671, 738, 1275, 1308, 1599, 1612
FauI CCCGC 1 cut(s) 1754
FauNDI CATATG 1 cut(s) 216
FbaI TGATCA 2 cut(s) 885, 1023
FokI GGATG 3 cut(s) 98, 955, 979
FriOI GRGCYC 1 cut(s) 1407
FspBI CTAG 3 cut(s) 527, 983, 1524
GsaI CCCAGC 2 cut(s) 406, 1342
HaeIII GGCC 2 cut(s) 697, 1343
HapII CCGG 4 cut(s) 305, 753, 1473, 1750
HgaI GACGC 1 cut(s) 1465
Hin1I GRCGYC 1 cut(s) 1476
Hin1II CATG 9 cut(s) 173, 347, 511, 675, 742, 1279, 1312, 1603, 1616
HinfI GANTC 6 cut(s) 238, 925, 979, 1439, 1651, 1740
HpaII CCGG 4 cut(s) 305, 753, 1473, 1750
Hpy166II GTNNAC 3 cut(s) 1199, 1289, 1531
Hpy188I TCNGA 7 cut(s) 192, 326, 577, 956, 1124, 1325, 1444
Hpy188III TCNNGA 5 cut(s) 462, 1042, 1156, 1655, 1715
Hpy8I GTNNAC 3 cut(s) 1199, 1289, 1531
HpyAV CCTTC 7 cut(s) 64, 98, 197, 375, 548, 1221, 1354
HpyCH4III ACNGT 1 cut(s) 1093
HpyCH4IV ACGT 1 cut(s) 1077
HpyF10VI GCNNNNNNNGC 8 cut(s) 137, 232, 301, 412, 1058, 1461, 1482, 1520
HpyF3I CTNAG 7 cut(s) 141, 375, 411, 699, 1048, 1121, 1226
HpySE526I ACGT 1 cut(s) 1077
Hsp92I GRCGYC 1 cut(s) 1476
Hsp92II CATG 9 cut(s) 173, 347, 511, 675, 742, 1279, 1312, 1603, 1616
KroI GCCGGC 1 cut(s) 304
KroNI GCCGGC 1 cut(s) 306
Ksp22I TGATCA 2 cut(s) 885, 1023
Kzo9I GATC 4 cut(s) 849, 885, 1023, 1687
LmnI GCTCC 2 cut(s) 995, 1402
Lsp1109I GCAGC 7 cut(s) 52, 149, 213, 271, 527, 625, 1494
LweI GCATC 5 cut(s) 581, 650, 933, 1326, 1394
MaeI CTAG 3 cut(s) 527, 983, 1524
MaeII ACGT 1 cut(s) 1077
MaeIII GTNAC 1 cut(s) 1628
MalI GATC 4 cut(s) 851, 887, 1025, 1689
MboI GATC 4 cut(s) 849, 885, 1023, 1687
MboII GAAGA 4 cut(s) 134, 491, 1277, 1312
MfeI CAATTG 2 cut(s) 1215, 1431
MhlI GDGCHC 3 cut(s) 1291, 1407, 1533
MlyI GAGTC 1 cut(s) 1749
MmeI TCCRAC 4 cut(s) 266, 827, 934, 1467
MroNI GCCGGC 1 cut(s) 304
MseI TTAA 5 cut(s) 153, 159, 588, 650, 1247
MslI CAYNNNNRTG 4 cut(s) 348, 670, 1505, 1617
MspA1I CMGCKG 3 cut(s) 140, 859, 1485
MspI CCGG 4 cut(s) 305, 753, 1473, 1750
MspR9I CCNGG 1 cut(s) 1399
MunI CAATTG 2 cut(s) 1215, 1431
Mva1269I GAATGC 2 cut(s) 406, 1134
MvaI CCWGG 1 cut(s) 1399
MwoI GCNNNNNNNGC 8 cut(s) 137, 232, 301, 412, 1058, 1461, 1482, 1520
NaeI GCCGGC 1 cut(s) 306
NcoI CCATGG 1 cut(s) 1599
NdeI CATATG 1 cut(s) 216
NdeII GATC 4 cut(s) 849, 885, 1023, 1687
NgoMIV GCCGGC 1 cut(s) 304
NlaIII CATG 9 cut(s) 173, 347, 511, 675, 742, 1279, 1312, 1603, 1616
NlaIV GGNNCC 3 cut(s) 7, 997, 1428
NmeAIII GCCGAG 1 cut(s) 622
NspI RCATGY 1 cut(s) 1616
PaeR7I CTCGAG 1 cut(s) 1737
PctI GAATGC 2 cut(s) 406, 1134
PdiI GCCGGC 1 cut(s) 306
PfeI GAWTC 5 cut(s) 238, 925, 979, 1439, 1651
PflMI CCANNNNNTGG 1 cut(s) 939
PleI GAGTC 1 cut(s) 1748
PpsI GAGTC 1 cut(s) 1748
PshBI ATTAAT 2 cut(s) 153, 159
Psp124BI GAGCTC 1 cut(s) 1407
Psp1406I AACGTT 1 cut(s) 1077
Psp6I CCWGG 1 cut(s) 1397
PspFI CCCAGC 2 cut(s) 402, 1338
PspGI CCWGG 1 cut(s) 1397
PspN4I GGNNCC 3 cut(s) 7, 997, 1428
PspPI GGNCC 2 cut(s) 726, 1341
PspXI VCTCGAGB 1 cut(s) 1737
PstI CTGCAG 2 cut(s) 231, 1484
PstNI CAGNNNCTG 2 cut(s) 17, 235
PvuII CAGCTG 1 cut(s) 140
RsaI GTAC 1 cut(s) 1209
RsaNI GTAC 1 cut(s) 1208
RseI CAYNNNNRTG 4 cut(s) 348, 670, 1505, 1617
SacI GAGCTC 1 cut(s) 1407
SaqAI TTAA 5 cut(s) 153, 159, 588, 650, 1247
Sau3AI GATC 4 cut(s) 849, 885, 1023, 1687
Sau96I GGNCC 2 cut(s) 726, 1341
SchI GAGTC 1 cut(s) 1749
ScrFI CCNGG 1 cut(s) 1399
SduI GDGCHC 3 cut(s) 1291, 1407, 1533
SfaNI GCATC 5 cut(s) 581, 650, 933, 1326, 1394
SfcI CTRYAG 2 cut(s) 227, 1480
Sfr274I CTCGAG 1 cut(s) 1737
SinI GGWCC 1 cut(s) 726
SlaI CTCGAG 1 cut(s) 1737
SmiMI CAYNNNNRTG 4 cut(s) 348, 670, 1505, 1617
SmlI CTYRAG 5 cut(s) 44, 1042, 1165, 1256, 1737
SmoI CTYRAG 5 cut(s) 44, 1042, 1165, 1256, 1737
SspI AATATT 1 cut(s) 157
SspMI CTAG 3 cut(s) 527, 983, 1524
SstI GAGCTC 1 cut(s) 1407
StyD4I CCNGG 1 cut(s) 1397
StyI CCWWGG 2 cut(s) 1534, 1599
TaaI ACNGT 1 cut(s) 1093
TaiI ACGT 1 cut(s) 1080
TaqI TCGA 4 cut(s) 633, 897, 1714, 1738
TauI GCSGC 6 cut(s) 11, 298, 301, 304, 1488, 1514
TfiI GAWTC 5 cut(s) 238, 925, 979, 1439, 1651
Tru1I TTAA 5 cut(s) 153, 159, 588, 650, 1247
Tru9I TTAA 5 cut(s) 153, 159, 588, 650, 1247
TscAI CASTG 4 cut(s) 459, 1026, 1230, 1365
TseI GCWGC 7 cut(s) 40, 137, 226, 284, 515, 638, 1482
TspDTI ATGAA 6 cut(s) 203, 701, 706, 1264, 1336, 1718
TspRI CASTG 4 cut(s) 459, 1026, 1230, 1365
Van91I CCANNNNNTGG 1 cut(s) 939
VneI GTGCAC 2 cut(s) 1287, 1529
VpaK11BI GGWCC 1 cut(s) 726
VspI ATTAAT 2 cut(s) 153, 159
XapI RAATTY 4 cut(s) 457, 756, 1003, 1249
XceI RCATGY 1 cut(s) 1616
XhoI CTCGAG 1 cut(s) 1737
XspI CTAG 3 cut(s) 527, 983, 1524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.