Rh1DG037600

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0002251
Physical Location & Seq
Forward (+)
6058062 .. 6061961
3900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG037600.1

Sequence Viewer

Length: 561 bp
ATGGGTGCCGCTGAACACGTAGTGGTGGAGGGAGAAAGTGAAGAGGTTGAGGCGAGGGAGAGAGATAAGAGGGAGGAGAAGGAAGAGGAGGAGGAGAAAAGAGAGCTGGAAGTGAAAGATGTGGAGAAAGTTGAGGTGTTGGGTTTTGAACAACACAGCAATATTAATCATGATCAGAGTAGTCAGGGAGATGTTCATGTGTCAATGTTGCAGAGGCTTAATCCTACAAACCCTTTGAGAATTGCTATCAATGCTAAAACTCGTACTACTCCATCTCCATCTCTATCTGATTTCTCTCAGTCTCACACAACTCCTCCTCCTCAGCATTCTCAGCCTTGCTCTATTCCAACCCCTCAATGCATCTTCCGATATGGCAATGGCTGGGCCTTTGTTCAGATAGCAGCATATGGAAAAGATTTTGTGAGGGCATCACAAGAGACTTGGGAGCTCTTTGAAAGGCAAGGAATTCATCTCCTTGTTGATTCTGACATCACTAGCTCAATATGCTTCCTAACCGGCGTCTGCAGTGGCTCTATGTGTGTCATAGTTGTGGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

186

Amino Acids

20.78

Weight (kDa)

4.92

Isoelectric Point (pI)

44.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 118 - 183 3.9e-11 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 5
AciI CCGC 1 cut(s) 9
AcsI RAATTY 1 cut(s) 465
AcyI GRCGYC 1 cut(s) 519
AdeI CACNNNGTG 1 cut(s) 22
AfaI GTAC 1 cut(s) 265
AflIII ACRYGT 1 cut(s) 16
AgsI TTSAA 2 cut(s) 149, 455
AluBI AGCT 3 cut(s) 106, 448, 498
AluI AGCT 3 cut(s) 106, 448, 498
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 2 cut(s) 306, 431
AoxI GGCC 1 cut(s) 384
ApeKI GCWGC 2 cut(s) 401, 554
ApoI RAATTY 1 cut(s) 465
AseI ATTAAT 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 384
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 450
Bbv12I GWGCWC 1 cut(s) 450
BbvCI CCTCAGC 1 cut(s) 321
BbvI GCAGC 2 cut(s) 413, 541
BccI CCATC 2 cut(s) 280, 286
BclI TGATCA 1 cut(s) 172
BcoDI GTCTC 2 cut(s) 306, 431
BfaI CTAG 1 cut(s) 495
BfmI CTRYAG 1 cut(s) 523
BisI GCNGC 3 cut(s) 9, 402, 555
BlsI GCNGC 3 cut(s) 10, 403, 556
BmgT120I GGNCC 1 cut(s) 384
BmiI GGNNCC 1 cut(s) 7
BmsI GCATC 2 cut(s) 369, 437
Bpu10I CCTNAGC 1 cut(s) 321
BsaAI YACGTR 1 cut(s) 19
BsaHI GRCGYC 1 cut(s) 519
BsaXI ACNNNNNCTCC 6 cut(s) 259, 289, 298, 301, 328, 331
Bse118I RCCGGY 1 cut(s) 515
Bse3DI GCAATG 1 cut(s) 382
BseMI GCAATG 1 cut(s) 382
BseMII CTCAG 3 cut(s) 311, 335, 344
BseRI GAGGAG 7 cut(s) 89, 101, 104, 107, 303, 306, 309
BseXI GCAGC 2 cut(s) 413, 541
BseYI CCCAGC 1 cut(s) 381
BshFI GGCC 1 cut(s) 386
BshNI GGYRCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 450
BsiSI CCGG 1 cut(s) 516
BsmAI GTCTC 2 cut(s) 306, 431
BsmI GAATGC 1 cut(s) 325
BsnI GGCC 1 cut(s) 386
Bsp1286I GDGCHC 1 cut(s) 450
Bsp143I GATC 1 cut(s) 172
BspACI CCGC 1 cut(s) 9
BspANI GGCC 1 cut(s) 386
BspCNI CTCAG 3 cut(s) 310, 334, 343
BspHI TCATGA 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 7
BspMAI CTGCAG 1 cut(s) 527
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 1 cut(s) 382
BsrFI RCCGGY 1 cut(s) 515
BssAI RCCGGY 1 cut(s) 515
BssMI GATC 1 cut(s) 172
BssNI GRCGYC 1 cut(s) 519
Bst6I CTCTTC 2 cut(s) 36, 78
BstACI GRCGYC 1 cut(s) 519
BstBAI YACGTR 1 cut(s) 19
BstDEI CTNAG 4 cut(s) 297, 321, 330, 558
BstKTI GATC 1 cut(s) 175
BstMAI GTCTC 2 cut(s) 306, 431
BstMBI GATC 1 cut(s) 172
BstMWI GCNNNNNNNGC 3 cut(s) 251, 331, 504
BstSFI CTRYAG 1 cut(s) 523
BstV1I GCAGC 2 cut(s) 413, 541
BsuRI GGCC 1 cut(s) 386
BtsI GCAGTG 1 cut(s) 532
BtsIMutI CAGTG 1 cut(s) 532
CciI TCATGA 1 cut(s) 169
Cfr10I RCCGGY 1 cut(s) 515
Cfr13I GGNCC 1 cut(s) 384
CseI GACGC 1 cut(s) 508
Csp6I GTAC 1 cut(s) 264
CviAII CATG 2 cut(s) 170, 197
CviJI RGCY 9 cut(s) 106, 217, 334, 381, 386, 448, 498, 531, 554
CviKI_1 RGCY 9 cut(s) 106, 217, 334, 381, 386, 448, 498, 531, 554
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 4 cut(s) 297, 321, 330, 558
DpnI GATC 1 cut(s) 174
DpnII GATC 1 cut(s) 172
DraIII CACNNNGTG 1 cut(s) 22
Eam1104I CTCTTC 2 cut(s) 36, 78
EarI CTCTTC 2 cut(s) 36, 78
Ecl136II GAGCTC 1 cut(s) 448
Eco24I GRGCYC 1 cut(s) 450
Eco53kI GAGCTC 1 cut(s) 448
EcoICRI GAGCTC 1 cut(s) 448
EcoRI GAATTC 1 cut(s) 465
EcoT22I ATGCAT 1 cut(s) 362
EcoT38I GRGCYC 1 cut(s) 450
FaeI CATG 2 cut(s) 173, 200
FaiI YATR 8 cut(s) 171, 198, 372, 406, 408, 505, 536, 545
FatI CATG 2 cut(s) 169, 196
FauNDI CATATG 1 cut(s) 406
FbaI TGATCA 1 cut(s) 172
Fnu4HI GCNGC 3 cut(s) 9, 402, 555
FriOI GRGCYC 1 cut(s) 450
Fsp4HI GCNGC 3 cut(s) 9, 402, 555
FspBI CTAG 1 cut(s) 495
GluI GCNGC 3 cut(s) 9, 402, 555
GsaI CCCAGC 1 cut(s) 385
HaeIII GGCC 1 cut(s) 386
HapII CCGG 1 cut(s) 516
HgaI GACGC 1 cut(s) 508
Hin1I GRCGYC 1 cut(s) 519
Hin1II CATG 2 cut(s) 173, 200
HinfI GANTC 1 cut(s) 482
HpaII CCGG 1 cut(s) 516
Hpy188I TCNGA 5 cut(s) 177, 289, 368, 396, 487
Hpy188III TCNNGA 1 cut(s) 170
HpyAV CCTTC 1 cut(s) 73
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 3 cut(s) 211, 360, 525
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 331, 504
HpyF3I CTNAG 4 cut(s) 297, 321, 330, 558
HpySE526I ACGT 1 cut(s) 18
Hsp92I GRCGYC 1 cut(s) 519
Hsp92II CATG 2 cut(s) 173, 200
Ksp22I TGATCA 1 cut(s) 172
Kzo9I GATC 1 cut(s) 172
LmnI GCTCC 1 cut(s) 445
LpnPI CCDG 4 cut(s) 92, 170, 367, 529
Lsp1109I GCAGC 2 cut(s) 413, 541
LweI GCATC 2 cut(s) 369, 437
MaeI CTAG 1 cut(s) 495
MaeII ACGT 1 cut(s) 18
MalI GATC 1 cut(s) 174
MboI GATC 1 cut(s) 172
MboII GAAGA 3 cut(s) 53, 95, 355
MhlI GDGCHC 1 cut(s) 450
MluCI AATT 2 cut(s) 240, 465
MmeI TCCRAC 1 cut(s) 371
Mph1103I ATGCAT 1 cut(s) 362
MseI TTAA 2 cut(s) 165, 219
MslI CAYNNNNRTG 1 cut(s) 548
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 1 cut(s) 516
Mva1269I GAATGC 1 cut(s) 325
MwoI GCNNNNNNNGC 3 cut(s) 251, 331, 504
NdeI CATATG 1 cut(s) 406
NdeII GATC 1 cut(s) 172
NlaIII CATG 2 cut(s) 173, 200
NlaIV GGNNCC 1 cut(s) 7
NsiI ATGCAT 1 cut(s) 362
PagI TCATGA 1 cut(s) 169
PctI GAATGC 1 cut(s) 325
PfeI GAWTC 1 cut(s) 482
PkrI GCNGC 3 cut(s) 10, 403, 556
Ppu21I YACGTR 1 cut(s) 19
PshBI ATTAAT 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 450
PspFI CCCAGC 1 cut(s) 381
PspN4I GGNNCC 1 cut(s) 7
PspPI GGNCC 1 cut(s) 384
PstI CTGCAG 1 cut(s) 527
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
RseI CAYNNNNRTG 1 cut(s) 548
SacI GAGCTC 1 cut(s) 450
SaqAI TTAA 2 cut(s) 165, 219
SatI GCNGC 3 cut(s) 9, 402, 555
Sau3AI GATC 1 cut(s) 172
Sau96I GGNCC 1 cut(s) 384
SduI GDGCHC 1 cut(s) 450
SetI ASST 6 cut(s) 21, 48, 108, 138, 450, 500
SfaNI GCATC 2 cut(s) 369, 437
SfcI CTRYAG 1 cut(s) 523
SmiMI CAYNNNNRTG 1 cut(s) 548
Sse9I AATT 2 cut(s) 240, 465
SsiI CCGC 1 cut(s) 9
SspI AATATT 1 cut(s) 163
SspMI CTAG 1 cut(s) 495
SstI GAGCTC 1 cut(s) 450
TaiI ACGT 1 cut(s) 21
TasI AATT 2 cut(s) 240, 465
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 1 cut(s) 482
Tru1I TTAA 2 cut(s) 165, 219
Tru9I TTAA 2 cut(s) 165, 219
TscAI CASTG 1 cut(s) 532
TseI GCWGC 2 cut(s) 401, 554
TspDTI ATGAA 2 cut(s) 185, 458
TspRI CASTG 1 cut(s) 532
VspI ATTAAT 1 cut(s) 165
XapI RAATTY 1 cut(s) 465
XspI CTAG 1 cut(s) 495
Zsp2I ATGCAT 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.